AT3G17470

Region found in RelA / SpoT proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012431
Physical Location & Seq
Forward (+)
5979814 .. 5982145
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G17470.1

Sequence Viewer

Length: 1752 bp
ATGTCTGTGATTCGTCCTTCTCCGATTCCAATTCCGAGATGCAGATCTCAGGTACTGCATCGTCGTCTTTATTCGATTCAATTGATTCAGCGACGACGTCGACGGTGGAATCCGAGGTCGGAGGTGGAGGATACTGCGATTGAATCGACGGCTCGGTCTCCGGAGGCAGCGGGAGGGAAAATGGTGGTGGAGCTTGTTGGAGCTTTCAACGAAGTAACGGAGAGGATGAATTCGGTTTGGTTGTCGACGTCTTCGTCTAGGTTACTCTTTAAAGCTCTCAAGCTCTCGATTCCGATTTTACAGTCTCTTCCTCTTGCCTCTGATGGTCGCTCTCCTCTTTCTAAGGCTCTCTCACTCTCTATCATTCTCGCAGATCTCCAGATGGATGCAGAAGTTATCTCGGCGAGTATATTGAGTGAGGTGGTGGACGCGAATGCGATATCTATATATGAAGTTAGAGACCATATTGGTACTGGAACTGCTCATCTGCTTCATGAGATTTTTCGTGTGAAGAATATTCCTTTTAAAGTTGATGTGTTAGACGATGAAACTGCTGCTTCATTGAGAAAGTTCTATTTAACCTACTATGACATCAGAGCTGTGATTATGGATTTGGTTTCAAAGCTTGATGAGATGAGGCATTTGGATCACTTGCCAAGGTATCGCCAGCAGATTCTCTCTCTTGAAGTGTTGAAGATTTATTCTCCTTTAGCTCATGCGGTGGGGGCTAATCACCTGTCACTTGAGCTCGAGGACATCTCTTTCCGTTATCTTTTTCCGTGTTCGTATATATACTTAGATTCATGGTTAAGAGGTCACGAGAACGGGTCTAAACCGTTGATAGACGTGTACAAAGAGCAGCTTCATCGGTCTTTGAAGGATGATTTGGTTTTGGCTGAAATGGTGAATGATGTATATATTAAAGGTCGGTATAAAAGTCGGTACAGCATGATGAAGAAGCTCCTAAGAGATGGGCGTAAACCGGAAGAAGTGAACGATGTGCTTGGTCTACGGGTCATATTAATGCCTAATTCTGTAGTAAACGATGTTGAAGTTGGCGAAAAAGCCTGTTACAGAACAAGTGAGATTATCCGGTCTTTGTGGAAGGAGATTCCGCATAGAACAAAAGACTACATTGCTAGGCCAAAGGAAAATGGGTATAGGAGTTTACACATGGCAGTTGATGTTAGCGACAGCGATCAGATAAGACCTTTGATGGAGATACAAATACGGACAATGGATATGGATGGATCCGCTAATGCTGGAACAGCATCACATTCTTTGTATAAAGGCGGTCTAACCGACCCAAAAGAGGCGAAGCGGCTTAAAGCGATAATGTTGGCAGCAGCGGATCTTGCAGCGATTCGTCTCAAAGATATCTCATCAAATAAACACCAAAGCTTCAAGACAACAACGAACCAGAGAGACAGAGTGTTTTGCCTTCTGGACAAAAACGGGGACGGAATGATCAGCATAGAGGAACTAATGGAAGTGATGGAAGAGCTTGGAGCTCCAGGTGAAGACGCAGAGGAGATGATGCAGCTTCTTGACTCTAACAGCGATGGATCTCTTAGTTCAGACGAATTCGATACGTTTCAGAAACAAGTAGAGTTCATGCGTAAATGGGAAGATAGAGATAACGAGTACAAGAGTCTTTTAGACGAGAAGCTTCATGATCTTCCACATCAAGATACCACAGGTTTGATACAGTTATACAATAAAGAGCTTGAGGATCGTCTCTCAACCCATTAA

Protein Analysis

583

Amino Acids

66.58

Weight (kDa)

5.99

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 105 - 242 7.5e-22 HD domain
RelA_SpoT PF04607 309 - 431 1.7e-31 Region found in RelA / SpoT proteins
EF-hand_1 PF00036 476 - 501 8.5e-06 EF hand domain
EF-hand_7 PF13499 477 - 529 4.4e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17470 AT3G17470 AT3G17470
fragaria_vesca FvH4_4g21520 FvH4_4g21520 FvH4_4g21520
malus_domestica MD13G1139600.v1.1
prunus_persica Prupe.1G105100_v2.0.a1
pyrus_communis pycom13g12150
rosa_chinensis RchiOBHm_Chr4g0427541
rosa_laevigata RLG00000007228
rosa_multiflora Rmu_sc0009627.1_g000004
rosa_roxburghii Rroxscaffold_5G00369190
rosa_rugosa Rorug04G0218400
rosa_samantha Rh4AG275000 Rh4BG280800 Rh4CG296100 Rh4DG278200
rosa_wichuraiana Rw4G023840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 154, 253
AatII GACGTC 2 cut(s) 100, 251
AccI GTMKAC 3 cut(s) 100, 245, 1009
AccII CGCG 1 cut(s) 431
AccIII TCCGGA 1 cut(s) 160
AciI CCGC 7 cut(s) 170, 719, 1115, 1254, 1293, 1321, 1349
AclWI GGATC 6 cut(s) 654, 1245, 1258, 1359, 1573, 1740
AcsI RAATTY 2 cut(s) 229, 1583
AcyI GRCGYC 2 cut(s) 97, 248
AfaI GTAC 5 cut(s) 54, 472, 851, 944, 1646
AfiI CCNNNNNNNGG 1 cut(s) 1312
AflIII ACRYGT 1 cut(s) 846
AgsI TTSAA 9 cut(s) 80, 143, 208, 621, 686, 694, 877, 1052, 1405
AjiI CACGTC 1 cut(s) 847
AjnI CCWGG 1 cut(s) 1513
AjuI GAANNNNNNNTTGG 2 cut(s) 869, 901
Alw21I GWGCWC 2 cut(s) 750, 1513
Alw26I GTCTC 6 cut(s) 162, 309, 453, 1373, 1419, 1742
AlwI GGATC 6 cut(s) 654, 1245, 1258, 1359, 1573, 1740
AlwNI CAGNNNCTG 1 cut(s) 55
Ama87I CYCGRG 1 cut(s) 749
Aor13HI TCCGGA 1 cut(s) 160
AoxI GGCC 1 cut(s) 1142
ApeKI GCWGC 7 cut(s) 167, 554, 859, 1343, 1346, 1358, 1540
ApoI RAATTY 2 cut(s) 229, 1583
AseI ATTAAT 1 cut(s) 1022
AsuHPI GGTGA 3 cut(s) 725, 916, 1529
AvaI CYCGRG 1 cut(s) 749
BamHI GGATCC 1 cut(s) 1250
BanII GRGCYC 2 cut(s) 750, 1513
BauI CACGAG 1 cut(s) 818
BbsI GAAGAC 2 cut(s) 243, 1527
Bbv12I GWGCWC 2 cut(s) 750, 1513
BbvI GCAGC 7 cut(s) 179, 541, 871, 1355, 1358, 1370, 1552
BccI CCATC 7 cut(s) 317, 376, 965, 1210, 1241, 1489, 1556
BceAI ACGGC 1 cut(s) 165
BcgI CGANNNNNNTGC 4 cut(s) 533, 567, 848, 882
BciT130I CCWGG 1 cut(s) 1515
BciVI GTATCC 1 cut(s) 124
BclI TGATCA 1 cut(s) 1467
BcoDI GTCTC 6 cut(s) 162, 309, 453, 1373, 1419, 1742
BfaI CTAG 2 cut(s) 258, 1140
BfmI CTRYAG 1 cut(s) 1035
BfuI GTATCC 1 cut(s) 124
BglII AGATCT 2 cut(s) 44, 373
BisI GCNGC 8 cut(s) 168, 555, 860, 1322, 1344, 1347, 1359, 1541
BlsI GCNGC 8 cut(s) 169, 556, 861, 1323, 1345, 1348, 1360, 1542
Bme1390I CCNGG 1 cut(s) 1515
BmeT110I CYCGRG 1 cut(s) 749
BmgBI CACGTC 1 cut(s) 847
BmiI GGNNCC 1 cut(s) 1252
BmrFI CCNGG 1 cut(s) 1515
BmsI GCATC 5 cut(s) 29, 67, 376, 1280, 1527
BpiI GAAGAC 2 cut(s) 243, 1527
BplI GAGNNNNNCTC 2 cut(s) 743, 775
BpmI CTGGAG 2 cut(s) 362, 1497
BpuEI CTTGAG 3 cut(s) 263, 764, 1748
BsaBI GATNNNNATC 1 cut(s) 43
BsaHI GRCGYC 2 cut(s) 97, 248
BsaI GGTCTC 2 cut(s) 162, 453
BsaJI CCNNGG 2 cut(s) 113, 656
BsaWI WCCGGW 3 cut(s) 160, 982, 1092
Bsc4I CCNNNNNNNGG 1 cut(s) 1312
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 1134
Bse8I GATNNNNATC 1 cut(s) 43
BseAI TCCGGA 1 cut(s) 160
BseBI CCWGG 1 cut(s) 1515
BseDI CCNNGG 2 cut(s) 113, 656
BseGI GGATG 4 cut(s) 231, 391, 886, 1252
BseJI GATNNNNATC 1 cut(s) 43
BseLI CCNNNNNNNGG 1 cut(s) 1312
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 2 cut(s) 324, 1544
BseXI GCAGC 7 cut(s) 179, 541, 871, 1355, 1358, 1370, 1552
Bsh1236I CGCG 1 cut(s) 431
BshFI GGCC 1 cut(s) 1144
BsiHKAI GWGCWC 2 cut(s) 750, 1513
BsiHKCI CYCGRG 1 cut(s) 749
BsiSI CCGG 3 cut(s) 161, 983, 1093
BslFI GGGAC 1 cut(s) 1472
BslI CCNNNNNNNGG 1 cut(s) 1312
BsmAI GTCTC 6 cut(s) 162, 309, 453, 1373, 1419, 1742
BsmBI CGTCTC 2 cut(s) 1373, 1742
BsmFI GGGAC 1 cut(s) 1472
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 1 cut(s) 1144
Bso31I GGTCTC 2 cut(s) 162, 453
BsoBI CYCGRG 1 cut(s) 749
Bsp1286I GDGCHC 2 cut(s) 750, 1513
Bsp13I TCCGGA 1 cut(s) 160
Bsp1407I TGTACA 1 cut(s) 849
BspACI CCGC 7 cut(s) 170, 719, 1115, 1254, 1293, 1321, 1349
BspANI GGCC 1 cut(s) 1144
BspCNI CTCAG 1 cut(s) 61
BspEI TCCGGA 1 cut(s) 160
BspFNI CGCG 1 cut(s) 431
BspHI TCATGA 2 cut(s) 493, 1672
BspLI GGNNCC 1 cut(s) 1252
BspPI GGATC 6 cut(s) 654, 1245, 1258, 1359, 1573, 1740
BspQI GCTCTTC 1 cut(s) 1494
BspTNI GGTCTC 2 cut(s) 162, 453
BsrDI GCAATG 1 cut(s) 1134
BsrGI TGTACA 1 cut(s) 849
BsrI ACTGG 1 cut(s) 478
BssECI CCNNGG 2 cut(s) 113, 656
BssNI GRCGYC 2 cut(s) 97, 248
BssSI CACGAG 1 cut(s) 818
BssT1I CCWWGG 1 cut(s) 656
Bst2BI CACGAG 1 cut(s) 818
Bst2UI CCWGG 1 cut(s) 1515
Bst4CI ACNGT 4 cut(s) 105, 303, 837, 1710
Bst6I CTCTTC 2 cut(s) 312, 1494
BstACI GRCGYC 2 cut(s) 97, 248
BstAUI TGTACA 1 cut(s) 849
BstC8I GCNNGC 1 cut(s) 668
BstDEI CTNAG 5 cut(s) 48, 342, 796, 965, 1571
BstF5I GGATG 4 cut(s) 231, 391, 886, 1252
BstFNI CGCG 1 cut(s) 431
BstMAI GTCTC 6 cut(s) 162, 309, 453, 1373, 1419, 1742
BstMWI GCNNNNNNNGC 3 cut(s) 725, 1268, 1355
BstNI CCWGG 1 cut(s) 1515
BstSCI CCNGG 1 cut(s) 1513
BstSFI CTRYAG 1 cut(s) 1035
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 7 cut(s) 179, 541, 871, 1355, 1358, 1370, 1552
BstV2I GAAGAC 2 cut(s) 243, 1527
BstX2I RGATCY 5 cut(s) 44, 373, 1250, 1351, 1565
BstYI RGATCY 5 cut(s) 44, 373, 1250, 1351, 1565
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 1 cut(s) 1144
BtgZI GCGATG 1 cut(s) 1575
BtrI CACGTC 1 cut(s) 847
BtsCI GGATG 4 cut(s) 231, 391, 886, 1252
Cac8I GCNNGC 1 cut(s) 668
CaiI CAGNNNCTG 1 cut(s) 55
CciI TCATGA 2 cut(s) 493, 1672
CseI GACGC 2 cut(s) 437, 1532
Csp6I GTAC 5 cut(s) 53, 471, 850, 943, 1645
CviAII CATG 7 cut(s) 494, 716, 804, 949, 1174, 1615, 1673
CviQI GTAC 5 cut(s) 53, 471, 850, 943, 1645
DdeI CTNAG 5 cut(s) 48, 342, 796, 965, 1571
DraI TTTAAA 2 cut(s) 271, 526
DrdI GACNNNNNNGTC 2 cut(s) 154, 253
DseDI GACNNNNNNGTC 2 cut(s) 154, 253
Eam1104I CTCTTC 2 cut(s) 312, 1494
EarI CTCTTC 2 cut(s) 312, 1494
Ecl136II GAGCTC 2 cut(s) 748, 1511
Eco130I CCWWGG 1 cut(s) 656
Eco24I GRGCYC 2 cut(s) 750, 1513
Eco31I GGTCTC 2 cut(s) 162, 453
Eco32I GATATC 2 cut(s) 441, 1378
Eco53kI GAGCTC 2 cut(s) 748, 1511
Eco88I CYCGRG 1 cut(s) 749
EcoICRI GAGCTC 2 cut(s) 748, 1511
EcoRI GAATTC 2 cut(s) 229, 1583
EcoRII CCWGG 1 cut(s) 1513
EcoRV GATATC 2 cut(s) 441, 1378
EcoT14I CCWWGG 1 cut(s) 656
EcoT38I GRGCYC 2 cut(s) 750, 1513
ErhI CCWWGG 1 cut(s) 656
Esp3I CGTCTC 2 cut(s) 1373, 1742
FaeI CATG 7 cut(s) 497, 719, 807, 952, 1177, 1618, 1676
FalI AAGNNNNNCTT 2 cut(s) 846, 878
FaqI GGGAC 1 cut(s) 1472
FatI CATG 7 cut(s) 493, 715, 803, 948, 1173, 1614, 1672
FauI CCCGC 1 cut(s) 163
FbaI TGATCA 1 cut(s) 1467
FblI GTMKAC 3 cut(s) 100, 245, 1009
Fnu4HI GCNGC 8 cut(s) 168, 555, 860, 1322, 1344, 1347, 1359, 1541
FokI GGATG 4 cut(s) 238, 398, 893, 1259
FriOI GRGCYC 2 cut(s) 750, 1513
Fsp4HI GCNGC 8 cut(s) 168, 555, 860, 1322, 1344, 1347, 1359, 1541
FspBI CTAG 2 cut(s) 258, 1140
GluI GCNGC 8 cut(s) 168, 555, 860, 1322, 1344, 1347, 1359, 1541
GsuI CTGGAG 2 cut(s) 362, 1497
HaeIII GGCC 1 cut(s) 1144
HapII CCGG 3 cut(s) 161, 983, 1093
HgaI GACGC 2 cut(s) 437, 1532
Hin1I GRCGYC 2 cut(s) 97, 248
Hin1II CATG 7 cut(s) 497, 719, 807, 952, 1177, 1618, 1676
HincII GTYRAC 2 cut(s) 101, 246
HindII GTYRAC 2 cut(s) 101, 246
HindIII AAGCTT 3 cut(s) 623, 1399, 1667
HpaII CCGG 3 cut(s) 161, 983, 1093
HphI GGTGA 3 cut(s) 725, 916, 1529
Hpy166II GTNNAC 9 cut(s) 101, 246, 427, 850, 980, 994, 1010, 1042, 1169
Hpy8I GTNNAC 9 cut(s) 101, 246, 427, 850, 980, 994, 1010, 1042, 1169
Hpy99I CGWCG 7 cut(s) 66, 96, 99, 102, 105, 151, 250
HpyAV CCTTC 4 cut(s) 27, 871, 1099, 1451
HpyCH4III ACNGT 4 cut(s) 105, 303, 837, 1710
HpyCH4IV ACGT 4 cut(s) 97, 248, 846, 1592
HpyCH4V TGCA 5 cut(s) 42, 58, 389, 1358, 1540
HpyF10VI GCNNNNNNNGC 3 cut(s) 725, 1268, 1355
HpyF3I CTNAG 5 cut(s) 48, 342, 796, 965, 1571
HpySE526I ACGT 4 cut(s) 97, 248, 846, 1592
Hsp92I GRCGYC 2 cut(s) 97, 248
Hsp92II CATG 7 cut(s) 497, 719, 807, 952, 1177, 1618, 1676
Kpn2I TCCGGA 1 cut(s) 160
Ksp22I TGATCA 1 cut(s) 1467
LguI GCTCTTC 1 cut(s) 1494
LmnI GCTCC 5 cut(s) 190, 200, 966, 1508, 1516
Lsp1109I GCAGC 7 cut(s) 179, 541, 871, 1355, 1358, 1370, 1552
LweI GCATC 5 cut(s) 29, 67, 376, 1280, 1527
MaeI CTAG 2 cut(s) 258, 1140
MaeII ACGT 4 cut(s) 97, 248, 846, 1592
MaeIII GTNAC 5 cut(s) 214, 261, 738, 815, 1070
MfeI CAATTG 1 cut(s) 80
MflI RGATCY 5 cut(s) 44, 373, 1250, 1351, 1565
MhlI GDGCHC 2 cut(s) 750, 1513
MluCI AATT 5 cut(s) 30, 80, 229, 1030, 1583
MlyI GAGTC 2 cut(s) 1544, 1660
MmeI TCCRAC 2 cut(s) 99, 178
MroI TCCGGA 1 cut(s) 160
MseI TTAA 8 cut(s) 270, 525, 578, 809, 921, 1022, 1326, 1750
MslI CAYNNNNRTG 1 cut(s) 1022
MspA1I CMGCKG 2 cut(s) 170, 1349
MspI CCGG 3 cut(s) 161, 983, 1093
MspR9I CCNGG 1 cut(s) 1515
MunI CAATTG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 1 cut(s) 1515
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 3 cut(s) 725, 1268, 1355
NlaIII CATG 7 cut(s) 497, 719, 807, 952, 1177, 1618, 1676
NlaIV GGNNCC 1 cut(s) 1252
NmeAIII GCCGAG 1 cut(s) 380
NmuCI GTSAC 2 cut(s) 738, 815
PaeR7I CTCGAG 1 cut(s) 749
PagI TCATGA 2 cut(s) 493, 1672
PciSI GCTCTTC 1 cut(s) 1494
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PctI GAATGC 1 cut(s) 439
PflFI GACNNNGTC 1 cut(s) 96
PkrI GCNGC 8 cut(s) 169, 556, 861, 1323, 1345, 1348, 1360, 1542
PleI GAGTC 2 cut(s) 1544, 1659
PpsI GAGTC 2 cut(s) 1544, 1659
PshBI ATTAAT 1 cut(s) 1022
Psp124BI GAGCTC 2 cut(s) 750, 1513
Psp6I CCWGG 1 cut(s) 1513
PspGI CCWGG 1 cut(s) 1513
PspN4I GGNNCC 1 cut(s) 1252
PspXI VCTCGAGB 1 cut(s) 749
PstNI CAGNNNCTG 1 cut(s) 55
PsuI RGATCY 5 cut(s) 44, 373, 1250, 1351, 1565
PsyI GACNNNGTC 1 cut(s) 96
RsaI GTAC 5 cut(s) 54, 472, 851, 944, 1646
RsaNI GTAC 5 cut(s) 53, 471, 850, 943, 1645
RseI CAYNNNNRTG 1 cut(s) 1022
SacI GAGCTC 2 cut(s) 750, 1513
SalI GTCGAC 2 cut(s) 99, 244
SapI GCTCTTC 1 cut(s) 1494
SaqAI TTAA 8 cut(s) 270, 525, 578, 809, 921, 1022, 1326, 1750
SatI GCNGC 8 cut(s) 168, 555, 860, 1322, 1344, 1347, 1359, 1541
SchI GAGTC 2 cut(s) 1544, 1660
ScrFI CCNGG 1 cut(s) 1515
SduI GDGCHC 2 cut(s) 750, 1513
SfaNI GCATC 5 cut(s) 29, 67, 376, 1280, 1527
SfcI CTRYAG 1 cut(s) 1035
Sfr274I CTCGAG 1 cut(s) 749
SgrDI CGTCGACG 1 cut(s) 99
SlaI CTCGAG 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 1022
SmlI CTYRAG 4 cut(s) 278, 743, 749, 1727
SmoI CTYRAG 4 cut(s) 278, 743, 749, 1727
Sse9I AATT 5 cut(s) 30, 80, 229, 1030, 1583
SsiI CCGC 7 cut(s) 170, 719, 1115, 1254, 1293, 1321, 1349
SspI AATATT 1 cut(s) 517
SspMI CTAG 2 cut(s) 258, 1140
SstI GAGCTC 2 cut(s) 750, 1513
StyD4I CCNGG 1 cut(s) 1513
StyI CCWWGG 1 cut(s) 656
TaaI ACNGT 4 cut(s) 105, 303, 837, 1710
TaiI ACGT 4 cut(s) 100, 251, 849, 1595
TaqI TCGA 7 cut(s) 74, 100, 146, 245, 287, 750, 1587
TaqII GACCGA 2 cut(s) 144, 858
TasI AATT 5 cut(s) 30, 80, 229, 1030, 1583
TatI WGTACW 2 cut(s) 849, 1644
TauI GCSGC 1 cut(s) 1324
Tru1I TTAA 8 cut(s) 270, 525, 578, 809, 921, 1022, 1326, 1750
Tru9I TTAA 8 cut(s) 270, 525, 578, 809, 921, 1022, 1326, 1750
TseFI GTSAC 2 cut(s) 738, 815
TseI GCWGC 7 cut(s) 167, 554, 859, 1343, 1346, 1358, 1540
Tsp45I GTSAC 2 cut(s) 738, 815
TspGWI ACGGA 5 cut(s) 233, 755, 768, 1246, 1476
Tth111I GACNNNGTC 1 cut(s) 96
VspI ATTAAT 1 cut(s) 1022
XapI RAATTY 2 cut(s) 229, 1583
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
XhoI CTCGAG 1 cut(s) 749
XmiI GTMKAC 3 cut(s) 100, 245, 1009
XspI CTAG 2 cut(s) 258, 1140
ZraI GACGTC 2 cut(s) 98, 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.