RchiOBHm_Chr4g0427541

Region found in RelA / SpoT proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012431
Physical Location & Seq
Forward (+)
52773469 .. 52775972
2504 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39646

Sequence Viewer

Length: 1737 bp
ATGGAGCTCTACTCTGCTCACCACCACTATCTCCTTCGTCATCAGGACCCAATACCCAGAGTACTCCTCCGCACTGTACGGGTATTCCACAGAACTCTGCCTCCTCAGAGATGTCACCGGCGGATTCGGCGGCGGTGTCTCTCAACAACGACGGCGGCCCTGATGGAACAGACGGGAGGGAAAATGGTGGTGGAGCTGGTTGGAGCTTTCAACGAGCTCACAGAAAGGATGGGCATGGTCTCCAGCAGCGGGTCCCACTTACTCTTCAAAGCTCTCAAGCTCTCGATTCCCTTACTCCGCGCCTTGCCTGTAACCCCGGACGGTCGTTCTTCACTCTCCAAGGCCTTGTCTTTTGCCTTAATTCTCGCAGACCTTCAGATGGATGCGGAGGTGATTTCAGCTGGGATGCTCAGACCAGTTCTGGAGGCCGGAGCGATTTCGATACTCGAGGTTCGAAACCAGATCGGTACCGGCACTGCTCATTTGTTACACGAGAGCTTGCGTGTGATGAAAATCCCAACTAAAGTTGATGTCTTGGATGATGATAGTGCTGCTGCGTTGAGAAAATTCTGCTTGACATATTATGATATGAGGGCTGTGATTTTGGACTTGGTTCTCAGGCTTGATATAATGAGGCACCTCGATCATCTTCCGAGGTATCAGCAGCAGTTGGTAGCTCTGGAGGTGATGAAGATTCATGCACCTTTAGCTCATGCTGTTGGGGCTAATTGGTTGTCATTGGAGCTTGAGGATTTGTCATTTCAGTACTTGTTTCCGAATTCGTATTTGTATGTGGATACATGGTTGAGGAGTCATGAGACTGGAAGTAGGCCTTTGGTGGATGTGTACAAGGAGCAGCTTTTGCATTCGTTGAAAGAGGATCCCTTGTTGGCGGGGCTGGTTGTGGATGTGTCAGTGAATGGGAGATATAAGAGTCGGTATAGCACCATGAAGAAGCTGTTGAAGGATGGCAGGAAGCCGGAACAGGTAAATGACGTGCTGGGGTTGCGAGTCATATTGGAGCCGAGGTCTGGAGAAGATGAGGCAGAAATGGGGGAAAGAGCATGTTATAGGACTCGTGAAGTTGTTCAGTCATTGTGGAAAGAGATGCCTCACAGGACAAAGGATTACATTGCTAGGCCTAAGGCGAATGGGTATAGGAGTTTGCATATGGCAGTTGATGTGAGTGACGGTGGCAAGAGTAGACCTTTAATGGAAATACAAATTAGGACTCAGGAGATGGATATGTTGGCAGATGCTGGGACAGCATCCCACTCTTTGTACAAGAGTGGTCTTACAGACCCTGAAGAGGCAAAGCGGCTCAAGGCCATAATGATGGCTGCGGCTGAACTTGCAGCATTTCGCCTTAAAGATCTTCCATCGGCCAATCATGAAGGCATAGAGACTGAGCAAAGTAAGAGTGATAGAGTGTTCCGCCTTCTTGACAAGAATGGTGATGGAAGAATCAATATTGAGGAACTTAAGGAAGTGATGGAAGAGCTCGGTGCTCCAGGGGAAGATGCATGCGAGATGATGCAACTCCTTGATGCTAATAGCGATGGTTCTCTTAGCTCAGATGAATTTGATTTGTTTCAGAAACAGGTCGAGTTTATGCGTAATTTTGAGGACAGAGATGAGCAGTACAAAACCGAATTGAATGAGAAACTTCAGATTGCAGAAAACAACAGCTTGATCCAGGTATATGCTGAAGATCTTAGCGGCAGCATTGCAAACTAG

Protein Analysis

578

Amino Acids

65.39

Weight (kDa)

5.99

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 92 - 241 6.4e-24 HD domain
RelA_SpoT PF04607 308 - 431 5.2e-31 Region found in RelA / SpoT proteins
EF-hand_1 PF00036 475 - 500 2.7e-07 EF hand domain
EF-hand_7 PF13499 476 - 528 6.6e-10 EF-hand domain pair
EF-hand_6 PF13405 476 - 502 5.8e-06 EF-hand domain
EF-hand_5 PF13202 477 - 498 2e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17470 AT3G17470 AT3G17470
fragaria_vesca FvH4_4g21520 FvH4_4g21520 FvH4_4g21520
malus_domestica MD13G1139600.v1.1
prunus_persica Prupe.1G105100_v2.0.a1
pyrus_communis pycom13g12150
rosa_chinensis RchiOBHm_Chr4g0427541
rosa_laevigata RLG00000007228
rosa_multiflora Rmu_sc0009627.1_g000004
rosa_roxburghii Rroxscaffold_5G00369190
rosa_rugosa Rorug04G0218400
rosa_samantha Rh4AG275000 Rh4BG280800 Rh4CG296100 Rh4DG278200
rosa_wichuraiana Rw4G023840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 2 cut(s) 467, 636
AccI GTMKAC 1 cut(s) 1204
AccII CGCG 1 cut(s) 300
AclWI GGATC 3 cut(s) 875, 888, 1687
AcoI YGGCCR 1 cut(s) 1383
AcsI RAATTY 3 cut(s) 566, 778, 1580
AcuI CTGAAG 4 cut(s) 359, 1326, 1652, 1728
AfaI GTAC 7 cut(s) 63, 78, 469, 767, 848, 1283, 1643
AfiI CCNNNNNNNGG 4 cut(s) 249, 379, 1031, 1309
AflII CTTAAG 1 cut(s) 1481
AgsI TTSAA 5 cut(s) 211, 268, 874, 964, 1657
AjiI CACGTC 1 cut(s) 997
AjnI CCWGG 2 cut(s) 1510, 1695
AloI GAACNNNNNNTCC 2 cut(s) 85, 117
Alw21I GWGCWC 4 cut(s) 9, 219, 1503, 1510
Alw26I GTCTC 4 cut(s) 143, 244, 812, 1397
AlwI GGATC 3 cut(s) 875, 888, 1687
AlwNI CAGNNNCTG 2 cut(s) 1259, 1304
Ama87I CYCGRG 1 cut(s) 446
AoxI GGCC 7 cut(s) 156, 342, 426, 830, 1139, 1326, 1383
ApeKI GCWGC 8 cut(s) 246, 551, 554, 664, 856, 1340, 1355, 1722
ApoI RAATTY 3 cut(s) 566, 778, 1580
Asp700I GAANNNNTTC 1 cut(s) 1086
Asp718I GGTACC 1 cut(s) 467
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 3 cut(s) 46, 157, 252
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 5 cut(s) 11, 107, 403, 697, 1466
AsuII TTCGAA 1 cut(s) 454
AvaI CYCGRG 1 cut(s) 446
AvaII GGWCC 2 cut(s) 46, 252
AxyI CCTNAGG 1 cut(s) 1143
BamHI GGATCC 1 cut(s) 880
BanI GGYRCC 2 cut(s) 467, 636
BanII GRGCYC 3 cut(s) 9, 219, 1503
BauI CACGAG 2 cut(s) 491, 1077
Bbv12I GWGCWC 4 cut(s) 9, 219, 1503, 1510
BbvI GCAGC 7 cut(s) 258, 538, 541, 676, 868, 1327, 1367
BceAI ACGGC 1 cut(s) 168
BciT130I CCWGG 2 cut(s) 1512, 1697
BciVI GTATCC 1 cut(s) 790
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 4 cut(s) 143, 244, 812, 1397
BfaI CTAG 2 cut(s) 1137, 1735
BfrI CTTAAG 1 cut(s) 1481
BfuI GTATCC 1 cut(s) 790
BglII AGATCT 2 cut(s) 1372, 1711
BmcAI AGTACT 2 cut(s) 63, 767
Bme1390I CCNGG 3 cut(s) 317, 1512, 1697
Bme18I GGWCC 2 cut(s) 46, 252
BmeT110I CYCGRG 1 cut(s) 446
BmgBI CACGTC 1 cut(s) 997
BmgT120I GGNCC 3 cut(s) 46, 157, 252
BmiI GGNNCC 7 cut(s) 48, 253, 254, 469, 638, 882, 1023
BmrFI CCNGG 3 cut(s) 317, 1512, 1697
BmsI GCATC 8 cut(s) 373, 396, 1098, 1246, 1277, 1510, 1524, 1537
BplI GAGNNNNNCTC 3 cut(s) 28, 51, 83
BpmI CTGGAG 5 cut(s) 226, 443, 701, 1053, 1494
Bpu14I TTCGAA 1 cut(s) 454
BpuEI CTTGAG 3 cut(s) 260, 767, 1307
BpuMI CCSGG 1 cut(s) 317
BsaBI GATNNNNATC 1 cut(s) 512
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 5 cut(s) 315, 339, 653, 1025, 1511
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 1013, 1043
Bsc4I CCNNNNNNNGG 4 cut(s) 249, 379, 1031, 1309
Bse118I RCCGGY 2 cut(s) 117, 470
Bse1I ACTGG 2 cut(s) 416, 826
Bse21I CCTNAGG 1 cut(s) 1143
Bse3DI GCAATG 2 cut(s) 1131, 1725
Bse8I GATNNNNATC 1 cut(s) 512
BseBI CCWGG 2 cut(s) 1512, 1697
BseDI CCNNGG 5 cut(s) 315, 339, 653, 1025, 1511
BseGI GGATG 8 cut(s) 234, 388, 411, 544, 847, 913, 973, 1268
BseJI GATNNNNATC 1 cut(s) 512
BseLI CCNNNNNNNGG 4 cut(s) 249, 379, 1031, 1309
BseMI GCAATG 2 cut(s) 1131, 1725
BseMII CTCAG 6 cut(s) 119, 424, 631, 1247, 1398, 1587
BseNI ACTGG 2 cut(s) 416, 826
BseRI GAGGAG 3 cut(s) 56, 93, 823
BseXI GCAGC 7 cut(s) 258, 538, 541, 676, 868, 1327, 1367
BseYI CCCAGC 3 cut(s) 401, 1000, 1259
Bsh1236I CGCG 1 cut(s) 300
Bsh1285I CGRYCG 1 cut(s) 325
BshFI GGCC 7 cut(s) 158, 344, 428, 832, 1141, 1328, 1385
BshNI GGYRCC 2 cut(s) 467, 636
BsiEI CGRYCG 1 cut(s) 325
BsiHKAI GWGCWC 4 cut(s) 9, 219, 1503, 1510
BsiHKCI CYCGRG 1 cut(s) 446
BsiSI CCGG 5 cut(s) 118, 317, 429, 471, 980
BslFI GGGAC 2 cut(s) 238, 1276
BslI CCNNNNNNNGG 4 cut(s) 249, 379, 1031, 1309
BsmAI GTCTC 4 cut(s) 143, 244, 812, 1397
BsmFI GGGAC 2 cut(s) 238, 1276
BsmI GAATGC 1 cut(s) 865
BsnI GGCC 7 cut(s) 158, 344, 428, 832, 1141, 1328, 1385
Bso31I GGTCTC 1 cut(s) 244
BsoBI CYCGRG 1 cut(s) 446
Bsp119I TTCGAA 1 cut(s) 454
Bsp1286I GDGCHC 4 cut(s) 9, 219, 1503, 1510
Bsp1407I TGTACA 2 cut(s) 846, 1281
Bsp143I GATC 6 cut(s) 462, 643, 880, 1372, 1692, 1711
BspANI GGCC 7 cut(s) 158, 344, 428, 832, 1141, 1328, 1385
BspCNI CTCAG 6 cut(s) 118, 423, 630, 1246, 1399, 1586
BspFNI CGCG 1 cut(s) 300
BspHI TCATGA 2 cut(s) 814, 1390
BspLI GGNNCC 7 cut(s) 48, 253, 254, 469, 638, 882, 1023
BspPI GGATC 3 cut(s) 875, 888, 1687
BspQI GCTCTTC 1 cut(s) 1491
BspT104I TTCGAA 1 cut(s) 454
BspT107I GGYRCC 2 cut(s) 467, 636
BspTI CTTAAG 1 cut(s) 1481
BspTNI GGTCTC 1 cut(s) 244
BsrDI GCAATG 2 cut(s) 1131, 1725
BsrFI RCCGGY 2 cut(s) 117, 470
BsrGI TGTACA 2 cut(s) 846, 1281
BsrI ACTGG 2 cut(s) 416, 826
BssAI RCCGGY 2 cut(s) 117, 470
BssECI CCNNGG 5 cut(s) 315, 339, 653, 1025, 1511
BssMI GATC 6 cut(s) 462, 643, 880, 1372, 1692, 1711
BssSI CACGAG 2 cut(s) 491, 1077
BssT1I CCWWGG 1 cut(s) 339
Bst2BI CACGAG 2 cut(s) 491, 1077
Bst2UI CCWGG 2 cut(s) 1512, 1697
Bst4CI ACNGT 3 cut(s) 76, 323, 1193
Bst6I CTCTTC 3 cut(s) 269, 1302, 1491
BstAFI CTTAAG 1 cut(s) 1481
BstAPI GCANNNNNTGC 1 cut(s) 862
BstAUI TGTACA 2 cut(s) 846, 1281
BstBI TTCGAA 1 cut(s) 454
BstC8I GCNNGC 2 cut(s) 500, 1525
BstDEI CTNAG 9 cut(s) 105, 410, 617, 1143, 1233, 1407, 1568, 1573, 1715
BstF5I GGATG 8 cut(s) 234, 388, 411, 544, 847, 913, 973, 1268
BstFNI CGCG 1 cut(s) 300
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 6 cut(s) 465, 646, 883, 1375, 1695, 1714
BstMAI GTCTC 4 cut(s) 143, 244, 812, 1397
BstMBI GATC 6 cut(s) 462, 643, 880, 1372, 1692, 1711
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 7 cut(s) 127, 707, 722, 862, 1006, 1265, 1352
BstNI CCWGG 2 cut(s) 1512, 1697
BstNSI RCATGY 2 cut(s) 1068, 1527
BstSCI CCNGG 3 cut(s) 315, 1510, 1695
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 7 cut(s) 258, 538, 541, 676, 868, 1327, 1367
BstX2I RGATCY 3 cut(s) 880, 1372, 1711
BstXI CCANNNNNNTGG 1 cut(s) 1336
BstYI RGATCY 3 cut(s) 880, 1372, 1711
Bsu36I CCTNAGG 1 cut(s) 1143
BsuI GTATCC 1 cut(s) 790
BsuRI GGCC 7 cut(s) 158, 344, 428, 832, 1141, 1328, 1385
BtgZI GCGATG 1 cut(s) 1572
BtrI CACGTC 1 cut(s) 997
BtsCI GGATG 8 cut(s) 234, 388, 411, 544, 847, 913, 973, 1268
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 3 cut(s) 72, 474, 921
Cac8I GCNNGC 2 cut(s) 500, 1525
CaiI CAGNNNCTG 2 cut(s) 1259, 1304
CciI TCATGA 2 cut(s) 814, 1390
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 2 cut(s) 117, 470
Cfr13I GGNCC 3 cut(s) 46, 157, 252
Csp6I GTAC 7 cut(s) 62, 77, 468, 766, 847, 1282, 1642
CviAII CATG 9 cut(s) 235, 698, 713, 801, 815, 949, 1065, 1391, 1524
CviQI GTAC 7 cut(s) 62, 77, 468, 766, 847, 1282, 1642
DdeI CTNAG 9 cut(s) 105, 410, 617, 1143, 1233, 1407, 1568, 1573, 1715
DpnI GATC 6 cut(s) 464, 645, 882, 1374, 1694, 1713
DpnII GATC 6 cut(s) 462, 643, 880, 1372, 1692, 1711
EaeI YGGCCR 1 cut(s) 1383
Eam1104I CTCTTC 3 cut(s) 269, 1302, 1491
EarI CTCTTC 3 cut(s) 269, 1302, 1491
EciI GGCGGA 2 cut(s) 136, 1424
Ecl136II GAGCTC 3 cut(s) 7, 217, 1501
Eco130I CCWWGG 1 cut(s) 339
Eco147I AGGCCT 3 cut(s) 344, 832, 1141
Eco24I GRGCYC 3 cut(s) 9, 219, 1503
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 46, 252
Eco53kI GAGCTC 3 cut(s) 7, 217, 1501
Eco57I CTGAAG 4 cut(s) 359, 1326, 1652, 1728
Eco81I CCTNAGG 1 cut(s) 1143
Eco88I CYCGRG 1 cut(s) 446
EcoICRI GAGCTC 3 cut(s) 7, 217, 1501
EcoO109I RGGNCCY 2 cut(s) 46, 252
EcoRI GAATTC 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 1510, 1695
EcoT14I CCWWGG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 1525
EcoT38I GRGCYC 3 cut(s) 9, 219, 1503
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 9 cut(s) 238, 701, 716, 804, 818, 952, 1068, 1394, 1527
FalI AAGNNNNNCTT 2 cut(s) 817, 849
FaqI GGGAC 2 cut(s) 238, 1276
FatI CATG 9 cut(s) 234, 697, 712, 800, 814, 948, 1064, 1390, 1523
FauI CCCGC 2 cut(s) 242, 886
FauNDI CATATG 1 cut(s) 1170
FblI GTMKAC 1 cut(s) 1204
FokI GGATG 8 cut(s) 241, 395, 418, 551, 854, 920, 980, 1255
FriOI GRGCYC 3 cut(s) 9, 219, 1503
FspBI CTAG 2 cut(s) 1137, 1735
GlaI GCGC 1 cut(s) 301
GsaI CCCAGC 3 cut(s) 405, 1004, 1263
GsuI CTGGAG 5 cut(s) 226, 443, 701, 1053, 1494
HaeIII GGCC 7 cut(s) 158, 344, 428, 832, 1141, 1328, 1385
HapII CCGG 5 cut(s) 118, 317, 429, 471, 980
HhaI GCGC 1 cut(s) 302
Hin1II CATG 9 cut(s) 238, 701, 716, 804, 818, 952, 1068, 1394, 1527
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HinfI GANTC 9 cut(s) 124, 286, 694, 811, 934, 1011, 1075, 1231, 1464
HpaII CCGG 5 cut(s) 118, 317, 429, 471, 980
HphI GGTGA 5 cut(s) 11, 107, 403, 697, 1466
Hpy166II GTNNAC 2 cut(s) 847, 1205
Hpy188I TCNGA 8 cut(s) 108, 378, 413, 654, 777, 1576, 1596, 1671
Hpy8I GTNNAC 2 cut(s) 847, 1205
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 5 cut(s) 44, 383, 958, 1388, 1448
HpyCH4III ACNGT 3 cut(s) 76, 323, 1193
HpyCH4IV ACGT 1 cut(s) 996
HpyCH4V TGCA 8 cut(s) 701, 865, 1168, 1355, 1523, 1537, 1676, 1730
HpyF10VI GCNNNNNNNGC 7 cut(s) 127, 707, 722, 862, 1006, 1265, 1352
HpyF3I CTNAG 9 cut(s) 105, 410, 617, 1143, 1233, 1407, 1568, 1573, 1715
HpySE526I ACGT 1 cut(s) 996
Hsp92II CATG 9 cut(s) 238, 701, 716, 804, 818, 952, 1068, 1394, 1527
HspAI GCGC 1 cut(s) 300
KflI GGGWCCC 1 cut(s) 252
KpnI GGTACC 1 cut(s) 471
Kzo9I GATC 6 cut(s) 462, 643, 880, 1372, 1692, 1711
LguI GCTCTTC 1 cut(s) 1491
LmnI GCTCC 8 cut(s) 4, 193, 203, 431, 742, 853, 1021, 1513
Lsp1109I GCAGC 7 cut(s) 258, 538, 541, 676, 868, 1327, 1367
LweI GCATC 8 cut(s) 373, 396, 1098, 1246, 1277, 1510, 1524, 1537
MaeI CTAG 2 cut(s) 1137, 1735
MaeII ACGT 1 cut(s) 996
MaeIII GTNAC 4 cut(s) 113, 310, 486, 1187
MalI GATC 6 cut(s) 464, 645, 882, 1374, 1694, 1713
MboI GATC 6 cut(s) 462, 643, 880, 1372, 1692, 1711
MflI RGATCY 3 cut(s) 880, 1372, 1711
MhlI GDGCHC 4 cut(s) 9, 219, 1503, 1510
MluCI AATT 8 cut(s) 360, 566, 727, 778, 1224, 1580, 1618, 1652
MlyI GAGTC 5 cut(s) 820, 943, 1020, 1069, 1225
MmeI TCCRAC 1 cut(s) 181
Mph1103I ATGCAT 1 cut(s) 1525
MroXI GAANNNNTTC 1 cut(s) 1086
MseI TTAA 4 cut(s) 359, 1211, 1368, 1482
MslI CAYNNNNRTG 1 cut(s) 1334
MspA1I CMGCKG 2 cut(s) 249, 401
MspCI CTTAAG 1 cut(s) 1481
MspI CCGG 5 cut(s) 118, 317, 429, 471, 980
MspR9I CCNGG 3 cut(s) 317, 1512, 1697
Mva1269I GAATGC 1 cut(s) 865
MvaI CCWGG 2 cut(s) 1512, 1697
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 7 cut(s) 127, 707, 722, 862, 1006, 1265, 1352
NciI CCSGG 1 cut(s) 317
NdeI CATATG 1 cut(s) 1170
NdeII GATC 6 cut(s) 462, 643, 880, 1372, 1692, 1711
NlaIII CATG 9 cut(s) 238, 701, 716, 804, 818, 952, 1068, 1394, 1527
NlaIV GGNNCC 7 cut(s) 48, 253, 254, 469, 638, 882, 1023
NmeAIII GCCGAG 1 cut(s) 1050
NmuCI GTSAC 2 cut(s) 113, 1187
NsiI ATGCAT 1 cut(s) 1525
NspI RCATGY 2 cut(s) 1068, 1527
NspV TTCGAA 1 cut(s) 454
PaeI GCATGC 1 cut(s) 1527
PaeR7I CTCGAG 1 cut(s) 446
PagI TCATGA 2 cut(s) 814, 1390
PceI AGGCCT 3 cut(s) 344, 832, 1141
PciSI GCTCTTC 1 cut(s) 1491
PctI GAATGC 1 cut(s) 865
PdmI GAANNNNTTC 1 cut(s) 1086
PfeI GAWTC 4 cut(s) 124, 286, 694, 1464
PleI GAGTC 5 cut(s) 819, 942, 1019, 1069, 1225
PpsI GAGTC 5 cut(s) 819, 942, 1019, 1069, 1225
PpuMI RGGWCCY 2 cut(s) 46, 252
Psp124BI GAGCTC 3 cut(s) 9, 219, 1503
Psp5II RGGWCCY 2 cut(s) 46, 252
Psp6I CCWGG 2 cut(s) 1510, 1695
PspFI CCCAGC 3 cut(s) 401, 1000, 1259
PspGI CCWGG 2 cut(s) 1510, 1695
PspN4I GGNNCC 7 cut(s) 48, 253, 254, 469, 638, 882, 1023
PspPI GGNCC 3 cut(s) 46, 157, 252
PspPPI RGGWCCY 2 cut(s) 46, 252
PspXI VCTCGAGB 1 cut(s) 446
PstNI CAGNNNCTG 2 cut(s) 1259, 1304
PsuI RGATCY 3 cut(s) 880, 1372, 1711
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 7 cut(s) 63, 78, 469, 767, 848, 1283, 1643
RsaNI GTAC 7 cut(s) 62, 77, 468, 766, 847, 1282, 1642
RseI CAYNNNNRTG 1 cut(s) 1334
SacI GAGCTC 3 cut(s) 9, 219, 1503
SapI GCTCTTC 1 cut(s) 1491
SaqAI TTAA 4 cut(s) 359, 1211, 1368, 1482
Sau3AI GATC 6 cut(s) 462, 643, 880, 1372, 1692, 1711
Sau96I GGNCC 3 cut(s) 46, 157, 252
ScaI AGTACT 2 cut(s) 63, 767
SchI GAGTC 5 cut(s) 820, 943, 1020, 1069, 1225
ScrFI CCNGG 3 cut(s) 317, 1512, 1697
SduI GDGCHC 4 cut(s) 9, 219, 1503, 1510
SfaNI GCATC 8 cut(s) 373, 396, 1098, 1246, 1277, 1510, 1524, 1537
Sfr274I CTCGAG 1 cut(s) 446
SfuI TTCGAA 1 cut(s) 454
SgrAI CRCCGGYG 1 cut(s) 117
SinI GGWCC 2 cut(s) 46, 252
SlaI CTCGAG 1 cut(s) 446
SmiMI CAYNNNNRTG 1 cut(s) 1334
SmlI CTYRAG 5 cut(s) 275, 446, 746, 1322, 1481
SmoI CTYRAG 5 cut(s) 275, 446, 746, 1322, 1481
SphI GCATGC 1 cut(s) 1527
Sse9I AATT 8 cut(s) 360, 566, 727, 778, 1224, 1580, 1618, 1652
SseBI AGGCCT 3 cut(s) 344, 832, 1141
SspI AATATT 1 cut(s) 1471
SspMI CTAG 2 cut(s) 1137, 1735
SstI GAGCTC 3 cut(s) 9, 219, 1503
StuI AGGCCT 3 cut(s) 344, 832, 1141
StyD4I CCNGG 3 cut(s) 315, 1510, 1695
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 3 cut(s) 76, 323, 1193
TaiI ACGT 1 cut(s) 999
TaqI TCGA 6 cut(s) 284, 440, 447, 454, 642, 1605
TasI AATT 8 cut(s) 360, 566, 727, 778, 1224, 1580, 1618, 1652
TatI WGTACW 5 cut(s) 61, 765, 846, 1281, 1641
TauI GCSGC 5 cut(s) 133, 158, 1321, 1346, 1722
TfiI GAWTC 4 cut(s) 124, 286, 694, 1464
Tru1I TTAA 4 cut(s) 359, 1211, 1368, 1482
Tru9I TTAA 4 cut(s) 359, 1211, 1368, 1482
TscAI CASTG 3 cut(s) 79, 481, 921
TseFI GTSAC 2 cut(s) 113, 1187
TseI GCWGC 8 cut(s) 246, 551, 554, 664, 856, 1340, 1355, 1722
Tsp45I GTSAC 2 cut(s) 113, 1187
TspDTI ATGAA 6 cut(s) 524, 686, 704, 965, 1407, 1593
TspRI CASTG 3 cut(s) 79, 481, 921
Vha464I CTTAAG 1 cut(s) 1481
VpaK11BI GGWCC 2 cut(s) 46, 252
XapI RAATTY 3 cut(s) 566, 778, 1580
XceI RCATGY 2 cut(s) 1068, 1527
XhoI CTCGAG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 1204
XmnI GAANNNNTTC 1 cut(s) 1086
XspI CTAG 2 cut(s) 1137, 1735
ZrmI AGTACT 2 cut(s) 63, 767
Zsp2I ATGCAT 1 cut(s) 1525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.