FvH4_4g21520

Region found in RelA / SpoT proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012431
Physical Location & Seq
Forward (+)
24572370 .. 24575224
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21520.t3

Sequence Viewer

Length: 1740 bp
ATGGAGCTCTACTCCGCTCACCACCACCACCATCTTCTCCGTCATCAGGACCCAATACCCAGATTGCTCCTCCGTACCCTACCCATCTTCCACAGCACCCTACCTCCACAGAGATGCCACCGTCAGCTTCGACGACGGCGCTCCTCAAAAACGACGGCGGCTCTGCTGGAACAGACGGGCGGGAAAATGGTGGTGGAGCTGGTCGGAGCTTTCAACGAGCTCACAGAAAGAATGGGCATGCTCTCCAGCAGCGGGTCCCACCTACTCTTCAAAGCTCTCAAGCTCTCCATCCCCTTGCTCTACGCCTTGCCTATAACCCCGGACGGCCGCTCCTCACTCTCCAAGGCCTTGTCTTTCGCCTTAATTCTCGCTGACCTCCAGATGGACGCGGAGGTGATTTCAGCTGGGATGCTGAGACCGGTTTTGGAAGCCGGAGCGATTTCGATACAGGAGGTGAGGGACCAGATTGGTACCGGAACGGCTCACTTGTTACACGAGAGCTTGCGTGTGATGAAAGTTCCTTCCAAGGTTGATGTGTTGGATGATGACAGTGCAGCTGCATTGAGGAAGTTTTGCTTGACGTATTATGACACGAGGGCTGTGATTCTGGACTTGGTTTTGCGGCTCGATATGATGAGGCATCTTGGTTATCTTCCGAGGTATCAGCAGCATTTGGTTGCTTTGGAGGTTATGAAGATTCATGCACCTTTAGCTCATGCTGTTGGGGCCAACTGGTTGTCGTTGGAGCTTGAGGATTTGTCGTTTCAGTACTTGTTTCCGAATTCGTATTTGTATGTGGATGAGTGGTTGAGGAGTCATGAGAGTGGAAGTAGGCCGTTGGTGGATGTGTACAAGGAGCAACTTTTGCAGTCGTTGAGAGAGGATCCTTTGTTGGCGGGGCTGATTGTAGGTGTTTCGGTGTATGGTAGGTATAAGAGTCGGTATAGCACCATGAAGAAGCTGTTGAAGGATGGGAGGAAGCCGGAGCAGGTGAATGATGTGTTGGGGTTGAGGGTTATATTGGAGCCGAAGTCTGGAGAAGATGAGGCCAAAATGGGGGAAAGAGCTTGTTACAGGACTCGTGAAGTGGTTCGGTCATTGTGGAAAGAGATGCCTCACAGGACAAAGGATTACATTGCTAGGCCTAAGGCGAATGGGTATAGGAGTTTGCATATGGCAGTTGATGTGAGTGACAGTGGCAAGAGTAGGCCTTTAATGGAAATACAAATTAGGACTCAGGAGATGGACATGTTGGCGGATGCTGGCACTGCATCTCACTCATTGTACAAGAGTGGTCTTACAAACCCAGAAGAGGCAAAGCGGCTCAAGGCCATAATGATGGCTGCAGCTGAACTTGCAGCATTTCGCCTTAAAGATCTTCCGTCAGCCAATCATGGAGGCATTGAGACTGAGCAAAGTAAGAGTGATAGAGTGTTCCGCCTTCTTGACAAGAATGGTGATGGAAGAATCAACTTTGAGGAACTTAAGGAAGTTATGGAAGAGCTCGGTGCCCCAGGGGAAGATGCATGCGAAATGATGCAACTGCTTGATGCTAATAGCGATGGTTCTCTTAGCTCAGACGAATTTGATTTGTTTCAGAAACAGGTCGAGTTTATGCGTAATTTTGTGGACAGAGATGAGCAGTACAAGACAGAATTGAATGAGAAACTTCAGATTGCAGAAAACAACAGCTTGATCCAGGTATATGCTGAAGATCTTAGTGGCACCATTGCAAACTAA

Protein Analysis

580

Amino Acids

65.42

Weight (kDa)

6.31

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 106 - 242 1.6e-22 HD domain
RelA_SpoT PF04607 309 - 432 2e-31 Region found in RelA / SpoT proteins
EF-hand_1 PF00036 476 - 501 5.4e-08 EF hand domain
EF-hand_7 PF13499 477 - 529 1e-09 EF-hand domain pair
EF-hand_6 PF13405 477 - 503 3.2e-06 EF-hand domain
EF-hand_5 PF13202 478 - 499 9.9e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17470 AT3G17470 AT3G17470
fragaria_vesca FvH4_4g21520 FvH4_4g21520 FvH4_4g21520
malus_domestica MD13G1139600.v1.1
prunus_persica Prupe.1G105100_v2.0.a1
pyrus_communis pycom13g12150
rosa_chinensis RchiOBHm_Chr4g0427541
rosa_laevigata RLG00000007228
rosa_multiflora Rmu_sc0009627.1_g000004
rosa_roxburghii Rroxscaffold_5G00369190
rosa_rugosa Rorug04G0218400
rosa_samantha Rh4AG275000 Rh4BG280800 Rh4CG296100 Rh4DG278200
rosa_wichuraiana Rw4G023840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 979
Acc36I ACCTGC 1 cut(s) 979
Acc65I GGTACC 1 cut(s) 470
AccB1I GGYRCC 3 cut(s) 470, 1508, 1724
AccBSI CCGCTC 2 cut(s) 17, 330
AccII CGCG 1 cut(s) 389
AclWI GGATC 3 cut(s) 878, 891, 1690
AcoI YGGCCR 1 cut(s) 325
AcsI RAATTY 2 cut(s) 781, 1583
AcuI CTGAAG 2 cut(s) 1655, 1731
AfaI GTAC 6 cut(s) 76, 472, 770, 851, 1286, 1646
AfiI CCNNNNNNNGG 6 cut(s) 46, 252, 382, 1034, 1056, 1312
AflII CTTAAG 1 cut(s) 1484
AflIII ACRYGT 1 cut(s) 1248
AgeI ACCGGT 1 cut(s) 418
AgsI TTSAA 4 cut(s) 214, 271, 967, 1660
AjnI CCWGG 2 cut(s) 1513, 1698
AjuI GAANNNNNNNTTGG 2 cut(s) 986, 1018
Alw21I GWGCWC 3 cut(s) 9, 222, 1506
Alw26I GTCTC 2 cut(s) 409, 1400
AlwI GGATC 3 cut(s) 878, 891, 1690
AoxI GGCC 8 cut(s) 325, 345, 726, 833, 1047, 1142, 1208, 1329
ApeKI GCWGC 7 cut(s) 249, 554, 557, 667, 1343, 1346, 1358
ApoI RAATTY 2 cut(s) 781, 1583
AsiGI ACCGGT 1 cut(s) 418
Asp700I GAANNNNTTC 1 cut(s) 1089
Asp718I GGTACC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 4 cut(s) 49, 255, 460, 726
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 5 cut(s) 11, 406, 466, 1003, 1469
AvaII GGWCC 3 cut(s) 49, 255, 460
AxyI CCTNAGG 1 cut(s) 1146
BaeGI GKGCMC 1 cut(s) 1513
BamHI GGATCC 1 cut(s) 883
BanI GGYRCC 3 cut(s) 470, 1508, 1724
BanII GRGCYC 3 cut(s) 9, 222, 1506
BauI CACGAG 3 cut(s) 494, 592, 1080
Bbv12I GWGCWC 3 cut(s) 9, 222, 1506
BbvI GCAGC 7 cut(s) 261, 544, 566, 679, 1330, 1358, 1370
BccI CCATC 9 cut(s) 39, 92, 296, 376, 965, 1237, 1333, 1454, 1556
BceAI ACGGC 5 cut(s) 152, 171, 340, 495, 820
BciT130I CCWGG 2 cut(s) 1515, 1700
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 409, 1400
BfaI CTAG 1 cut(s) 1140
BfmI CTRYAG 1 cut(s) 1344
BfoI RGCGCY 1 cut(s) 142
BfrI CTTAAG 1 cut(s) 1484
BfuAI ACCTGC 1 cut(s) 979
BglII AGATCT 2 cut(s) 1375, 1714
BmcAI AGTACT 1 cut(s) 770
Bme1390I CCNGG 3 cut(s) 320, 1515, 1700
Bme18I GGWCC 3 cut(s) 49, 255, 460
BmgT120I GGNCC 4 cut(s) 49, 255, 460, 726
BmrFI CCNGG 3 cut(s) 320, 1515, 1700
BmsI GCATC 9 cut(s) 104, 399, 649, 1101, 1249, 1280, 1513, 1527, 1540
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 3 cut(s) 229, 362, 1056
BpuEI CTTGAG 3 cut(s) 263, 770, 1310
BpuMI CCSGG 1 cut(s) 320
BsaI GGTCTC 1 cut(s) 409
BsaJI CCNNGG 6 cut(s) 318, 342, 525, 656, 1513, 1514
BsaWI WCCGGW 2 cut(s) 418, 473
BsaXI ACNNNNNCTCC 6 cut(s) 88, 118, 314, 344, 1016, 1046
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 252, 382, 1034, 1056, 1312
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 1 cut(s) 737
Bse21I CCTNAGG 1 cut(s) 1146
Bse3DI GCAATG 2 cut(s) 1134, 1728
BseBI CCWGG 2 cut(s) 1515, 1700
BseDI CCNNGG 6 cut(s) 318, 342, 525, 656, 1513, 1514
BseGI GGATG 7 cut(s) 288, 414, 547, 805, 850, 976, 1264
BseLI CCNNNNNNNGG 6 cut(s) 46, 252, 382, 1034, 1056, 1312
BseMI GCAATG 2 cut(s) 1134, 1728
BseMII CTCAG 4 cut(s) 404, 1250, 1401, 1590
BseNI ACTGG 1 cut(s) 737
BseRI GAGGAG 4 cut(s) 59, 133, 322, 826
BseSI GKGCMC 1 cut(s) 1513
BseX3I CGGCCG 1 cut(s) 325
BseXI GCAGC 7 cut(s) 261, 544, 566, 679, 1330, 1358, 1370
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 573
Bsh1236I CGCG 1 cut(s) 389
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 8 cut(s) 327, 347, 728, 835, 1049, 1144, 1210, 1331
BshNI GGYRCC 3 cut(s) 470, 1508, 1724
BshTI ACCGGT 1 cut(s) 418
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 3 cut(s) 9, 222, 1506
BsiSI CCGG 5 cut(s) 320, 419, 432, 474, 983
BslFI GGGAC 2 cut(s) 241, 473
BslI CCNNNNNNNGG 6 cut(s) 46, 252, 382, 1034, 1056, 1312
BsmAI GTCTC 2 cut(s) 409, 1400
BsmFI GGGAC 2 cut(s) 241, 473
BsnI GGCC 8 cut(s) 327, 347, 728, 835, 1049, 1144, 1210, 1331
Bso31I GGTCTC 1 cut(s) 409
Bsp1286I GDGCHC 4 cut(s) 9, 222, 1506, 1513
Bsp1407I TGTACA 2 cut(s) 849, 1284
Bsp143I GATC 4 cut(s) 883, 1375, 1695, 1714
BspANI GGCC 8 cut(s) 327, 347, 728, 835, 1049, 1144, 1210, 1331
BspCNI CTCAG 4 cut(s) 405, 1249, 1402, 1589
BspFNI CGCG 1 cut(s) 389
BspHI TCATGA 1 cut(s) 817
BspMAI CTGCAG 1 cut(s) 1348
BspMI ACCTGC 1 cut(s) 979
BspPI GGATC 3 cut(s) 878, 891, 1690
BspQI GCTCTTC 1 cut(s) 1494
BspT107I GGYRCC 3 cut(s) 470, 1508, 1724
BspTI CTTAAG 1 cut(s) 1484
BspTNI GGTCTC 1 cut(s) 409
BsrBI CCGCTC 2 cut(s) 17, 330
BsrDI GCAATG 2 cut(s) 1134, 1728
BsrFI RCCGGY 1 cut(s) 418
BsrGI TGTACA 2 cut(s) 849, 1284
BsrI ACTGG 1 cut(s) 737
BssAI RCCGGY 1 cut(s) 418
BssECI CCNNGG 6 cut(s) 318, 342, 525, 656, 1513, 1514
BssMI GATC 4 cut(s) 883, 1375, 1695, 1714
BssSI CACGAG 3 cut(s) 494, 592, 1080
BssT1I CCWWGG 2 cut(s) 342, 525
Bst2BI CACGAG 3 cut(s) 494, 592, 1080
Bst2UI CCWGG 2 cut(s) 1515, 1700
Bst4CI ACNGT 3 cut(s) 122, 551, 1196
Bst6I CTCTTC 3 cut(s) 272, 1305, 1494
BstAFI CTTAAG 1 cut(s) 1484
BstAPI GCANNNNNTGC 1 cut(s) 865
BstAUI TGTACA 2 cut(s) 849, 1284
BstC8I GCNNGC 4 cut(s) 239, 503, 1264, 1528
BstDEI CTNAG 7 cut(s) 413, 1146, 1236, 1410, 1571, 1576, 1718
BstF5I GGATG 7 cut(s) 288, 414, 547, 805, 850, 976, 1264
BstFNI CGCG 1 cut(s) 389
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 4 cut(s) 886, 1378, 1698, 1717
BstMAI GTCTC 2 cut(s) 409, 1400
BstMBI GATC 4 cut(s) 883, 1375, 1695, 1714
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 5 cut(s) 710, 725, 865, 1268, 1355
BstNI CCWGG 2 cut(s) 1515, 1700
BstNSI RCATGY 3 cut(s) 241, 1252, 1530
BstSCI CCNGG 3 cut(s) 318, 1513, 1698
BstSFI CTRYAG 1 cut(s) 1344
BstSLI GKGCMC 1 cut(s) 1513
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 7 cut(s) 261, 544, 566, 679, 1330, 1358, 1370
BstX2I RGATCY 3 cut(s) 883, 1375, 1714
BstXI CCANNNNNNTGG 1 cut(s) 1339
BstYI RGATCY 3 cut(s) 883, 1375, 1714
BstZI CGGCCG 1 cut(s) 325
Bsu36I CCTNAGG 1 cut(s) 1146
BsuRI GGCC 8 cut(s) 327, 347, 728, 835, 1049, 1144, 1210, 1331
BtgZI GCGATG 1 cut(s) 1575
BtsCI GGATG 7 cut(s) 288, 414, 547, 805, 850, 976, 1264
BtsI GCAGTG 1 cut(s) 1266
BtsIMutI CAGTG 3 cut(s) 556, 1201, 1266
BveI ACCTGC 1 cut(s) 979
Cac8I GCNNGC 4 cut(s) 239, 503, 1264, 1528
CciI TCATGA 1 cut(s) 817
CfoI GCGC 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 4 cut(s) 49, 255, 460, 726
CseI GACGC 1 cut(s) 395
Csp6I GTAC 6 cut(s) 75, 471, 769, 850, 1285, 1645
CspAI ACCGGT 1 cut(s) 418
CviAII CATG 8 cut(s) 238, 701, 716, 818, 952, 1249, 1394, 1527
CviQI GTAC 6 cut(s) 75, 471, 769, 850, 1285, 1645
DdeI CTNAG 7 cut(s) 413, 1146, 1236, 1410, 1571, 1576, 1718
DpnI GATC 4 cut(s) 885, 1377, 1697, 1716
DpnII GATC 4 cut(s) 883, 1375, 1695, 1714
EaeI YGGCCR 1 cut(s) 325
EagI CGGCCG 1 cut(s) 325
Eam1104I CTCTTC 3 cut(s) 272, 1305, 1494
EarI CTCTTC 3 cut(s) 272, 1305, 1494
EciI GGCGGA 2 cut(s) 1271, 1427
Ecl136II GAGCTC 3 cut(s) 7, 220, 1504
EclXI CGGCCG 1 cut(s) 325
Eco130I CCWWGG 2 cut(s) 342, 525
Eco147I AGGCCT 3 cut(s) 347, 1144, 1210
Eco24I GRGCYC 3 cut(s) 9, 222, 1506
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 3 cut(s) 49, 255, 460
Eco52I CGGCCG 1 cut(s) 325
Eco53kI GAGCTC 3 cut(s) 7, 220, 1504
Eco57I CTGAAG 2 cut(s) 1655, 1731
Eco81I CCTNAGG 1 cut(s) 1146
EcoICRI GAGCTC 3 cut(s) 7, 220, 1504
EcoO109I RGGNCCY 2 cut(s) 49, 255
EcoRI GAATTC 1 cut(s) 781
EcoRII CCWGG 2 cut(s) 1513, 1698
EcoT14I CCWWGG 2 cut(s) 342, 525
EcoT22I ATGCAT 1 cut(s) 1528
EcoT38I GRGCYC 3 cut(s) 9, 222, 1506
ErhI CCWWGG 2 cut(s) 342, 525
FaeI CATG 8 cut(s) 241, 704, 719, 821, 955, 1252, 1397, 1530
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 2 cut(s) 241, 473
FatI CATG 8 cut(s) 237, 700, 715, 817, 951, 1248, 1393, 1526
FauI CCCGC 3 cut(s) 173, 245, 889
FauNDI CATATG 1 cut(s) 1173
FokI GGATG 7 cut(s) 275, 421, 554, 812, 857, 983, 1271
FriOI GRGCYC 3 cut(s) 9, 222, 1506
FspBI CTAG 1 cut(s) 1140
GlaI GCGC 1 cut(s) 140
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 3 cut(s) 229, 362, 1056
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 8 cut(s) 327, 347, 728, 835, 1049, 1144, 1210, 1331
HapII CCGG 5 cut(s) 320, 419, 432, 474, 983
HgaI GACGC 1 cut(s) 395
HhaI GCGC 1 cut(s) 141
Hin1II CATG 8 cut(s) 241, 704, 719, 821, 955, 1252, 1397, 1530
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HinfI GANTC 7 cut(s) 604, 697, 814, 937, 1078, 1234, 1467
HpaII CCGG 5 cut(s) 320, 419, 432, 474, 983
HphI GGTGA 5 cut(s) 11, 406, 466, 1003, 1469
Hpy166II GTNNAC 2 cut(s) 850, 1630
Hpy188I TCNGA 6 cut(s) 206, 657, 780, 1579, 1599, 1674
Hpy188III TCNNGA 8 cut(s) 47, 379, 608, 818, 1035, 1082, 1238, 1445
Hpy8I GTNNAC 2 cut(s) 850, 1630
Hpy99I CGWCG 3 cut(s) 135, 138, 157
HpyAV CCTTC 3 cut(s) 531, 961, 1451
HpyCH4III ACNGT 3 cut(s) 122, 551, 1196
HpyCH4IV ACGT 1 cut(s) 581
HpyF10VI GCNNNNNNNGC 5 cut(s) 710, 725, 865, 1268, 1355
HpyF3I CTNAG 7 cut(s) 413, 1146, 1236, 1410, 1571, 1576, 1718
HpySE526I ACGT 1 cut(s) 581
Hsp92II CATG 8 cut(s) 241, 704, 719, 821, 955, 1252, 1397, 1530
HspAI GCGC 1 cut(s) 139
KflI GGGWCCC 1 cut(s) 255
KpnI GGTACC 1 cut(s) 474
Kzo9I GATC 4 cut(s) 883, 1375, 1695, 1714
LguI GCTCTTC 1 cut(s) 1494
Lsp1109I GCAGC 7 cut(s) 261, 544, 566, 679, 1330, 1358, 1370
LweI GCATC 9 cut(s) 104, 399, 649, 1101, 1249, 1280, 1513, 1527, 1540
MaeI CTAG 1 cut(s) 1140
MaeII ACGT 1 cut(s) 581
MaeIII GTNAC 3 cut(s) 489, 1070, 1190
MalI GATC 4 cut(s) 885, 1377, 1697, 1716
MbiI CCGCTC 2 cut(s) 17, 330
MboI GATC 4 cut(s) 883, 1375, 1695, 1714
MflI RGATCY 3 cut(s) 883, 1375, 1714
MhlI GDGCHC 4 cut(s) 9, 222, 1506, 1513
MluCI AATT 6 cut(s) 363, 781, 1227, 1583, 1621, 1655
MlyI GAGTC 4 cut(s) 823, 946, 1072, 1228
MmeI TCCRAC 3 cut(s) 184, 519, 723
Mph1103I ATGCAT 1 cut(s) 1528
MroXI GAANNNNTTC 1 cut(s) 1089
MseI TTAA 4 cut(s) 362, 1214, 1371, 1485
MslI CAYNNNNRTG 3 cut(s) 112, 822, 1337
MspA1I CMGCKG 4 cut(s) 252, 404, 557, 1349
MspCI CTTAAG 1 cut(s) 1484
MspI CCGG 5 cut(s) 320, 419, 432, 474, 983
MspR9I CCNGG 3 cut(s) 320, 1515, 1700
MvaI CCWGG 2 cut(s) 1515, 1700
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 5 cut(s) 710, 725, 865, 1268, 1355
NciI CCSGG 1 cut(s) 320
NdeI CATATG 1 cut(s) 1173
NdeII GATC 4 cut(s) 883, 1375, 1695, 1714
NlaIII CATG 8 cut(s) 241, 704, 719, 821, 955, 1252, 1397, 1530
NmuCI GTSAC 1 cut(s) 1190
NsiI ATGCAT 1 cut(s) 1528
NspI RCATGY 3 cut(s) 241, 1252, 1530
PaeI GCATGC 2 cut(s) 241, 1530
PagI TCATGA 1 cut(s) 817
PaqCI CACCTGC 1 cut(s) 979
PasI CCCWGGG 1 cut(s) 1514
PceI AGGCCT 3 cut(s) 347, 1144, 1210
PciI ACATGT 1 cut(s) 1248
PciSI GCTCTTC 1 cut(s) 1494
PdmI GAANNNNTTC 1 cut(s) 1089
PfeI GAWTC 3 cut(s) 604, 697, 1467
PinAI ACCGGT 1 cut(s) 418
PleI GAGTC 4 cut(s) 822, 945, 1072, 1228
PpsI GAGTC 4 cut(s) 822, 945, 1072, 1228
PpuMI RGGWCCY 2 cut(s) 49, 255
PscI ACATGT 1 cut(s) 1248
Psp124BI GAGCTC 3 cut(s) 9, 222, 1506
Psp5II RGGWCCY 2 cut(s) 49, 255
Psp6I CCWGG 2 cut(s) 1513, 1698
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 2 cut(s) 1513, 1698
PspPI GGNCC 4 cut(s) 49, 255, 460, 726
PspPPI RGGWCCY 2 cut(s) 49, 255
PstI CTGCAG 1 cut(s) 1348
PsuI RGATCY 3 cut(s) 883, 1375, 1714
PvuII CAGCTG 3 cut(s) 404, 557, 1349
RsaI GTAC 6 cut(s) 76, 472, 770, 851, 1286, 1646
RsaNI GTAC 6 cut(s) 75, 471, 769, 850, 1285, 1645
RseI CAYNNNNRTG 3 cut(s) 112, 822, 1337
SacI GAGCTC 3 cut(s) 9, 222, 1506
SapI GCTCTTC 1 cut(s) 1494
SaqAI TTAA 4 cut(s) 362, 1214, 1371, 1485
Sau3AI GATC 4 cut(s) 883, 1375, 1695, 1714
Sau96I GGNCC 4 cut(s) 49, 255, 460, 726
ScaI AGTACT 1 cut(s) 770
SchI GAGTC 4 cut(s) 823, 946, 1072, 1228
ScrFI CCNGG 3 cut(s) 320, 1515, 1700
SduI GDGCHC 4 cut(s) 9, 222, 1506, 1513
SfaNI GCATC 9 cut(s) 104, 399, 649, 1101, 1249, 1280, 1513, 1527, 1540
SfcI CTRYAG 1 cut(s) 1344
SinI GGWCC 3 cut(s) 49, 255, 460
SmiMI CAYNNNNRTG 3 cut(s) 112, 822, 1337
SmlI CTYRAG 4 cut(s) 278, 749, 1325, 1484
SmoI CTYRAG 4 cut(s) 278, 749, 1325, 1484
SphI GCATGC 2 cut(s) 241, 1530
Sse9I AATT 6 cut(s) 363, 781, 1227, 1583, 1621, 1655
SseBI AGGCCT 3 cut(s) 347, 1144, 1210
SspMI CTAG 1 cut(s) 1140
SstI GAGCTC 3 cut(s) 9, 222, 1506
StuI AGGCCT 3 cut(s) 347, 1144, 1210
StyD4I CCNGG 3 cut(s) 318, 1513, 1698
StyI CCWWGG 2 cut(s) 342, 525
TaaI ACNGT 3 cut(s) 122, 551, 1196
TaiI ACGT 1 cut(s) 584
TaqI TCGA 4 cut(s) 130, 443, 627, 1608
TaqII GACCGA 1 cut(s) 1083
TasI AATT 6 cut(s) 363, 781, 1227, 1583, 1621, 1655
TatI WGTACW 4 cut(s) 768, 849, 1284, 1644
TauI GCSGC 4 cut(s) 161, 330, 625, 1324
TfiI GAWTC 3 cut(s) 604, 697, 1467
Tru1I TTAA 4 cut(s) 362, 1214, 1371, 1485
Tru9I TTAA 4 cut(s) 362, 1214, 1371, 1485
TscAI CASTG 3 cut(s) 556, 1201, 1273
TseFI GTSAC 1 cut(s) 1190
TseI GCWGC 7 cut(s) 249, 554, 557, 667, 1343, 1346, 1358
Tsp45I GTSAC 1 cut(s) 1190
TspDTI ATGAA 4 cut(s) 527, 689, 707, 968
TspGWI ACGGA 3 cut(s) 29, 62, 1371
TspRI CASTG 3 cut(s) 556, 1201, 1273
Vha464I CTTAAG 1 cut(s) 1484
VpaK11BI GGWCC 3 cut(s) 49, 255, 460
XapI RAATTY 2 cut(s) 781, 1583
XceI RCATGY 3 cut(s) 241, 1252, 1530
XmnI GAANNNNTTC 1 cut(s) 1089
XspI CTAG 1 cut(s) 1140
ZrmI AGTACT 1 cut(s) 770
Zsp2I ATGCAT 1 cut(s) 1528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.