Rh4CG296100

Region found in RelA / SpoT proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0012431
Physical Location & Seq
Forward (+)
55532677 .. 55534755
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG296100.1

Sequence Viewer

Length: 1725 bp
ATGGAGCTCTACTCTGCTCACCACCACTATCTCCTTCGTCGTCAGGACCCAATACCCAGATTACTCCTCCGCACTGTACGGGTATTCCACAGCACTCTGCCTCCTCAGAGATGTCACCGGCGGTGTCTCCCAATAACGACGGCGGCCCTGATGGAACAGACGGGAGGGAAAATGGTGGTGGAGCTGGTTGGAGCTTTCAACGAGCTCACAGAAAGGATGGGTATGCTCTCCAGCAGCGGGTCCCACTTACTCTTCAAAGCTCTCAAGCTCTCGATTCCCTTACTCCGCGCCTTGCCTATAACCCCGGACGGTCGTTCTTCACTCTCCAAGGCCTTGTCTTTTGCCTTAATACTCGCAGACCTTCAGATGGATGCGGAGGTGATTTCAGCTGGGATGCTCAGACCAGTTCTGGAGGCCGGAGCGATTTCGATACTCGAGGTTCGAAACCAGATCGGTACCGGCACTGCTCATTTGTTACACGAGAGCTTGCGTGTGATGAAAATCCCAACTAAAGTTGATGTCTTGGATGATGATAGTGCTGCTGCGTTGAGAAAATTTTGCTTGACATATTATGATATGAGGGCTGTGATTTTGGACTTGGTTCTCCGGCTTGATATAATGAGGCACCTCGATTATCTTCCGAGGTATCAGCAGCAGTTGGTAGCTCTGGAGGTGATGAAGATTCATGCACCTTTAGCTCATGCTGTTGGGGCTAATTGGTTGTCATTGGAGCTTGAGGATTTGTCATTTCAGTACTTGTTTCCGAATTCGTATTTGTATGTGGATACATGGTTGAGGAGTCATGAGACTGGAAGTAGGCCTTTGGTGGATGTGTACAAGGAGCAGCTTTTGCATTCGTTGAAAGAGGATCCCTTGTTGGCGGGGCTGGTTGTGGATGTGTCAGTGAATGGGAGATATAAGAGTCGGTATAGCACCATGAAGAAGCTGTTGAAGGATGGTAGGAAGCCGGAACAGGTAAATGACGTGCTGGGGTTGCGAGTCATATTGGAGCCGAGGTCTGGAGAAGATGAGGCAGAAATGGGGGAAAGAGCATGTTATAGGACTCGTCAAGTTGTTCAGTCATTGTGGAAAGAGATGCCTCACAGGACAAAGGATTACATTGCTAGGCCTAAAGCGAATGGGTATAGGAGTTTGCATATGGCAGTTGATGTGAGTGACGGTGGCAAGAGTAGACCTTTAATGGAAATACAAATAAGGACTCAGGAGATGGATATGTTGGCAGATGCTGGGACAGCATCCCACTCTTTGTACAAGAGTGGTCTTACAGACCCTGAAGAGGCAAAGCGGCTCAAGGCCATAATGATGGCTGCGGCTGAACTTGCAGCATTTCGCCTTAAAGATCTTCCATCGGCCAATCATGAAGGCATAGAGACTGAGCAAAATAAGAGTGATAGAGTGTTCCGCCTTCTTGACAAGAATGGTGATGGAAGAATCAATATTGAGGAACTTAAGGAAGTGATGGAAGAGCTCGGTGCTCCAGGGGAAGATGCATGCGAGATGATGCAACTCCTTGATGCTAATAGCGATGGTTCTCTTAGCTCAGATGAATTTGATTTGTTTCAGAAACAGGTCGAGTTCATGCGTAATTTTGAGGACAGAGATGATCAGTACAAAACCGAATTGAATGAGAAACTTCAGATTGCAGAAAACAACAGCTTGATCCAGGTATATGCTGAAGATCTTAGCGGCAGCATTGCAAACTAG

Protein Analysis

574

Amino Acids

64.86

Weight (kDa)

5.78

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 100 - 237 4.4e-23 HD domain
RelA_SpoT PF04607 304 - 427 6.4e-31 Region found in RelA / SpoT proteins
EF-hand_1 PF00036 471 - 496 2.6e-07 EF hand domain
EF-hand_7 PF13499 472 - 524 8.4e-10 EF-hand domain pair
EF-hand_6 PF13405 472 - 498 5.8e-06 EF-hand domain
EF-hand_5 PF13202 473 - 494 2e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17470 AT3G17470 AT3G17470
fragaria_vesca FvH4_4g21520 FvH4_4g21520 FvH4_4g21520
malus_domestica MD13G1139600.v1.1
prunus_persica Prupe.1G105100_v2.0.a1
pyrus_communis pycom13g12150
rosa_chinensis RchiOBHm_Chr4g0427541
rosa_laevigata RLG00000007228
rosa_multiflora Rmu_sc0009627.1_g000004
rosa_roxburghii Rroxscaffold_5G00369190
rosa_rugosa Rorug04G0218400
rosa_samantha Rh4AG275000 Rh4BG280800 Rh4CG296100 Rh4DG278200
rosa_wichuraiana Rw4G023840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 455
AccB1I GGYRCC 2 cut(s) 455, 624
AccI GTMKAC 1 cut(s) 1192
AccII CGCG 1 cut(s) 288
AclWI GGATC 3 cut(s) 863, 876, 1675
AcoI YGGCCR 1 cut(s) 1371
AcsI RAATTY 3 cut(s) 554, 766, 1568
AcuI CTGAAG 4 cut(s) 347, 1314, 1640, 1716
AfaI GTAC 6 cut(s) 78, 457, 755, 836, 1271, 1631
AfiI CCNNNNNNNGG 4 cut(s) 237, 367, 1019, 1297
AflII CTTAAG 1 cut(s) 1469
AgsI TTSAA 5 cut(s) 199, 256, 862, 952, 1645
AjiI CACGTC 1 cut(s) 985
AjnI CCWGG 2 cut(s) 1498, 1683
Alw21I GWGCWC 4 cut(s) 9, 207, 1491, 1498
Alw26I GTCTC 3 cut(s) 131, 800, 1385
AlwI GGATC 3 cut(s) 863, 876, 1675
AlwNI CAGNNNCTG 2 cut(s) 1247, 1292
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 7 cut(s) 144, 330, 414, 818, 1127, 1314, 1371
ApeKI GCWGC 8 cut(s) 234, 539, 542, 652, 844, 1328, 1343, 1710
ApoI RAATTY 3 cut(s) 554, 766, 1568
Asp718I GGTACC 1 cut(s) 455
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 3 cut(s) 46, 145, 240
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 5 cut(s) 11, 107, 391, 685, 1454
AsuII TTCGAA 1 cut(s) 442
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 2 cut(s) 46, 240
BamHI GGATCC 1 cut(s) 868
BanI GGYRCC 2 cut(s) 455, 624
BanII GRGCYC 3 cut(s) 9, 207, 1491
BauI CACGAG 1 cut(s) 479
Bbv12I GWGCWC 4 cut(s) 9, 207, 1491, 1498
BbvI GCAGC 7 cut(s) 246, 526, 529, 664, 856, 1315, 1355
BceAI ACGGC 1 cut(s) 156
BciT130I CCWGG 2 cut(s) 1500, 1685
BciVI GTATCC 1 cut(s) 778
BclI TGATCA 1 cut(s) 1624
BcnI CCSGG 1 cut(s) 305
BcoDI GTCTC 3 cut(s) 131, 800, 1385
BfaI CTAG 2 cut(s) 1125, 1723
BfrI CTTAAG 1 cut(s) 1469
BfuI GTATCC 1 cut(s) 778
BglII AGATCT 2 cut(s) 1360, 1699
BmcAI AGTACT 1 cut(s) 755
Bme1390I CCNGG 3 cut(s) 305, 1500, 1685
Bme18I GGWCC 2 cut(s) 46, 240
BmeT110I CYCGRG 1 cut(s) 434
BmgBI CACGTC 1 cut(s) 985
BmgT120I GGNCC 3 cut(s) 46, 145, 240
BmiI GGNNCC 7 cut(s) 48, 241, 242, 457, 626, 870, 1011
BmrFI CCNGG 3 cut(s) 305, 1500, 1685
BmsI GCATC 8 cut(s) 361, 384, 1086, 1234, 1265, 1498, 1512, 1525
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 5 cut(s) 214, 431, 689, 1041, 1482
Bpu14I TTCGAA 1 cut(s) 442
BpuEI CTTGAG 3 cut(s) 248, 755, 1295
BpuMI CCSGG 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 500
BsaJI CCNNGG 5 cut(s) 303, 327, 641, 1013, 1499
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 1001, 1031
Bsc4I CCNNNNNNNGG 4 cut(s) 237, 367, 1019, 1297
Bse118I RCCGGY 2 cut(s) 117, 458
Bse1I ACTGG 2 cut(s) 404, 814
Bse3DI GCAATG 2 cut(s) 1119, 1713
Bse8I GATNNNNATC 1 cut(s) 500
BseBI CCWGG 2 cut(s) 1500, 1685
BseDI CCNNGG 5 cut(s) 303, 327, 641, 1013, 1499
BseGI GGATG 8 cut(s) 222, 376, 399, 532, 835, 901, 961, 1256
BseJI GATNNNNATC 1 cut(s) 500
BseLI CCNNNNNNNGG 4 cut(s) 237, 367, 1019, 1297
BseMI GCAATG 2 cut(s) 1119, 1713
BseMII CTCAG 5 cut(s) 119, 412, 1235, 1386, 1575
BseNI ACTGG 2 cut(s) 404, 814
BseRI GAGGAG 3 cut(s) 56, 93, 811
BseXI GCAGC 7 cut(s) 246, 526, 529, 664, 856, 1315, 1355
BseYI CCCAGC 3 cut(s) 389, 988, 1247
Bsh1236I CGCG 1 cut(s) 288
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 7 cut(s) 146, 332, 416, 820, 1129, 1316, 1373
BshNI GGYRCC 2 cut(s) 455, 624
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 4 cut(s) 9, 207, 1491, 1498
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 6 cut(s) 118, 305, 417, 459, 607, 968
BslFI GGGAC 2 cut(s) 226, 1264
BslI CCNNNNNNNGG 4 cut(s) 237, 367, 1019, 1297
BsmAI GTCTC 3 cut(s) 131, 800, 1385
BsmFI GGGAC 2 cut(s) 226, 1264
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 7 cut(s) 146, 332, 416, 820, 1129, 1316, 1373
BsoBI CYCGRG 1 cut(s) 434
Bsp119I TTCGAA 1 cut(s) 442
Bsp1286I GDGCHC 4 cut(s) 9, 207, 1491, 1498
Bsp1407I TGTACA 2 cut(s) 834, 1269
Bsp143I GATC 6 cut(s) 450, 868, 1360, 1624, 1680, 1699
BspANI GGCC 7 cut(s) 146, 332, 416, 820, 1129, 1316, 1373
BspCNI CTCAG 5 cut(s) 118, 411, 1234, 1387, 1574
BspFNI CGCG 1 cut(s) 288
BspHI TCATGA 2 cut(s) 802, 1378
BspLI GGNNCC 7 cut(s) 48, 241, 242, 457, 626, 870, 1011
BspPI GGATC 3 cut(s) 863, 876, 1675
BspQI GCTCTTC 1 cut(s) 1479
BspT104I TTCGAA 1 cut(s) 442
BspT107I GGYRCC 2 cut(s) 455, 624
BspTI CTTAAG 1 cut(s) 1469
BsrDI GCAATG 2 cut(s) 1119, 1713
BsrFI RCCGGY 2 cut(s) 117, 458
BsrGI TGTACA 2 cut(s) 834, 1269
BsrI ACTGG 2 cut(s) 404, 814
BssAI RCCGGY 2 cut(s) 117, 458
BssECI CCNNGG 5 cut(s) 303, 327, 641, 1013, 1499
BssMI GATC 6 cut(s) 450, 868, 1360, 1624, 1680, 1699
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 2 cut(s) 1500, 1685
Bst4CI ACNGT 3 cut(s) 76, 311, 1181
Bst6I CTCTTC 3 cut(s) 257, 1290, 1479
BstAFI CTTAAG 1 cut(s) 1469
BstAPI GCANNNNNTGC 1 cut(s) 850
BstAUI TGTACA 2 cut(s) 834, 1269
BstBI TTCGAA 1 cut(s) 442
BstC8I GCNNGC 2 cut(s) 488, 1513
BstDEI CTNAG 7 cut(s) 105, 398, 1221, 1395, 1556, 1561, 1703
BstF5I GGATG 8 cut(s) 222, 376, 399, 532, 835, 901, 961, 1256
BstFNI CGCG 1 cut(s) 288
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 6 cut(s) 453, 871, 1363, 1627, 1683, 1702
BstMAI GTCTC 3 cut(s) 131, 800, 1385
BstMBI GATC 6 cut(s) 450, 868, 1360, 1624, 1680, 1699
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 6 cut(s) 695, 710, 850, 994, 1253, 1340
BstNI CCWGG 2 cut(s) 1500, 1685
BstNSI RCATGY 2 cut(s) 1056, 1515
BstSCI CCNGG 3 cut(s) 303, 1498, 1683
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 7 cut(s) 246, 526, 529, 664, 856, 1315, 1355
BstX2I RGATCY 3 cut(s) 868, 1360, 1699
BstXI CCANNNNNNTGG 1 cut(s) 1324
BstYI RGATCY 3 cut(s) 868, 1360, 1699
BsuI GTATCC 1 cut(s) 778
BsuRI GGCC 7 cut(s) 146, 332, 416, 820, 1129, 1316, 1373
BtgZI GCGATG 1 cut(s) 1560
BtrI CACGTC 1 cut(s) 985
BtsCI GGATG 8 cut(s) 222, 376, 399, 532, 835, 901, 961, 1256
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 3 cut(s) 72, 462, 909
Cac8I GCNNGC 2 cut(s) 488, 1513
CaiI CAGNNNCTG 2 cut(s) 1247, 1292
CciI TCATGA 2 cut(s) 802, 1378
CfoI GCGC 1 cut(s) 290
Cfr10I RCCGGY 2 cut(s) 117, 458
Cfr13I GGNCC 3 cut(s) 46, 145, 240
Csp6I GTAC 6 cut(s) 77, 456, 754, 835, 1270, 1630
CviAII CATG 9 cut(s) 686, 701, 789, 803, 937, 1053, 1379, 1512, 1600
CviQI GTAC 6 cut(s) 77, 456, 754, 835, 1270, 1630
DdeI CTNAG 7 cut(s) 105, 398, 1221, 1395, 1556, 1561, 1703
DpnI GATC 6 cut(s) 452, 870, 1362, 1626, 1682, 1701
DpnII GATC 6 cut(s) 450, 868, 1360, 1624, 1680, 1699
EaeI YGGCCR 1 cut(s) 1371
Eam1104I CTCTTC 3 cut(s) 257, 1290, 1479
EarI CTCTTC 3 cut(s) 257, 1290, 1479
EciI GGCGGA 1 cut(s) 1412
Ecl136II GAGCTC 3 cut(s) 7, 205, 1489
Eco130I CCWWGG 1 cut(s) 327
Eco147I AGGCCT 3 cut(s) 332, 820, 1129
Eco24I GRGCYC 3 cut(s) 9, 207, 1491
Eco47I GGWCC 2 cut(s) 46, 240
Eco53kI GAGCTC 3 cut(s) 7, 205, 1489
Eco57I CTGAAG 4 cut(s) 347, 1314, 1640, 1716
Eco88I CYCGRG 1 cut(s) 434
EcoICRI GAGCTC 3 cut(s) 7, 205, 1489
EcoO109I RGGNCCY 2 cut(s) 46, 240
EcoRI GAATTC 1 cut(s) 766
EcoRII CCWGG 2 cut(s) 1498, 1683
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 1 cut(s) 1513
EcoT38I GRGCYC 3 cut(s) 9, 207, 1491
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 9 cut(s) 689, 704, 792, 806, 940, 1056, 1382, 1515, 1603
FalI AAGNNNNNCTT 2 cut(s) 805, 837
FaqI GGGAC 2 cut(s) 226, 1264
FatI CATG 9 cut(s) 685, 700, 788, 802, 936, 1052, 1378, 1511, 1599
FauI CCCGC 2 cut(s) 230, 874
FauNDI CATATG 1 cut(s) 1158
FbaI TGATCA 1 cut(s) 1624
FblI GTMKAC 1 cut(s) 1192
FokI GGATG 8 cut(s) 229, 383, 406, 539, 842, 908, 968, 1243
FriOI GRGCYC 3 cut(s) 9, 207, 1491
FspBI CTAG 2 cut(s) 1125, 1723
GlaI GCGC 1 cut(s) 289
GsaI CCCAGC 3 cut(s) 393, 992, 1251
GsuI CTGGAG 5 cut(s) 214, 431, 689, 1041, 1482
HaeIII GGCC 7 cut(s) 146, 332, 416, 820, 1129, 1316, 1373
HapII CCGG 6 cut(s) 118, 305, 417, 459, 607, 968
HhaI GCGC 1 cut(s) 290
Hin1II CATG 9 cut(s) 689, 704, 792, 806, 940, 1056, 1382, 1515, 1603
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HinfI GANTC 8 cut(s) 274, 682, 799, 922, 999, 1063, 1219, 1452
HpaII CCGG 6 cut(s) 118, 305, 417, 459, 607, 968
HphI GGTGA 5 cut(s) 11, 107, 391, 685, 1454
Hpy166II GTNNAC 2 cut(s) 835, 1193
Hpy188I TCNGA 8 cut(s) 108, 366, 401, 642, 765, 1564, 1584, 1659
Hpy188III TCNNGA 9 cut(s) 44, 271, 410, 668, 803, 1020, 1223, 1379, 1430
Hpy8I GTNNAC 2 cut(s) 835, 1193
Hpy99I CGWCG 2 cut(s) 42, 142
HpyAV CCTTC 5 cut(s) 44, 371, 946, 1376, 1436
HpyCH4III ACNGT 3 cut(s) 76, 311, 1181
HpyCH4IV ACGT 1 cut(s) 984
HpyCH4V TGCA 8 cut(s) 689, 853, 1156, 1343, 1511, 1525, 1664, 1718
HpyF10VI GCNNNNNNNGC 6 cut(s) 695, 710, 850, 994, 1253, 1340
HpyF3I CTNAG 7 cut(s) 105, 398, 1221, 1395, 1556, 1561, 1703
HpySE526I ACGT 1 cut(s) 984
Hsp92II CATG 9 cut(s) 689, 704, 792, 806, 940, 1056, 1382, 1515, 1603
HspAI GCGC 1 cut(s) 288
KflI GGGWCCC 1 cut(s) 240
KpnI GGTACC 1 cut(s) 459
Ksp22I TGATCA 1 cut(s) 1624
Kzo9I GATC 6 cut(s) 450, 868, 1360, 1624, 1680, 1699
LguI GCTCTTC 1 cut(s) 1479
LmnI GCTCC 8 cut(s) 4, 181, 191, 419, 730, 841, 1009, 1501
Lsp1109I GCAGC 7 cut(s) 246, 526, 529, 664, 856, 1315, 1355
LweI GCATC 8 cut(s) 361, 384, 1086, 1234, 1265, 1498, 1512, 1525
MaeI CTAG 2 cut(s) 1125, 1723
MaeII ACGT 1 cut(s) 984
MaeIII GTNAC 3 cut(s) 113, 474, 1175
MalI GATC 6 cut(s) 452, 870, 1362, 1626, 1682, 1701
MboI GATC 6 cut(s) 450, 868, 1360, 1624, 1680, 1699
MflI RGATCY 3 cut(s) 868, 1360, 1699
MhlI GDGCHC 4 cut(s) 9, 207, 1491, 1498
MluCI AATT 6 cut(s) 554, 715, 766, 1568, 1606, 1640
MlyI GAGTC 5 cut(s) 808, 931, 1008, 1057, 1213
MmeI TCCRAC 1 cut(s) 169
Mph1103I ATGCAT 1 cut(s) 1513
MseI TTAA 4 cut(s) 347, 1199, 1356, 1470
MslI CAYNNNNRTG 1 cut(s) 1322
MspA1I CMGCKG 2 cut(s) 237, 389
MspCI CTTAAG 1 cut(s) 1469
MspI CCGG 6 cut(s) 118, 305, 417, 459, 607, 968
MspR9I CCNGG 3 cut(s) 305, 1500, 1685
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 2 cut(s) 1500, 1685
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 6 cut(s) 695, 710, 850, 994, 1253, 1340
NciI CCSGG 1 cut(s) 305
NdeI CATATG 1 cut(s) 1158
NdeII GATC 6 cut(s) 450, 868, 1360, 1624, 1680, 1699
NlaIII CATG 9 cut(s) 689, 704, 792, 806, 940, 1056, 1382, 1515, 1603
NlaIV GGNNCC 7 cut(s) 48, 241, 242, 457, 626, 870, 1011
NmeAIII GCCGAG 1 cut(s) 1038
NmuCI GTSAC 2 cut(s) 113, 1175
NsiI ATGCAT 1 cut(s) 1513
NspI RCATGY 2 cut(s) 1056, 1515
NspV TTCGAA 1 cut(s) 442
PaeI GCATGC 1 cut(s) 1515
PaeR7I CTCGAG 1 cut(s) 434
PagI TCATGA 2 cut(s) 802, 1378
PceI AGGCCT 3 cut(s) 332, 820, 1129
PciSI GCTCTTC 1 cut(s) 1479
PctI GAATGC 1 cut(s) 853
PfeI GAWTC 3 cut(s) 274, 682, 1452
PleI GAGTC 5 cut(s) 807, 930, 1007, 1057, 1213
PpsI GAGTC 5 cut(s) 807, 930, 1007, 1057, 1213
PpuMI RGGWCCY 2 cut(s) 46, 240
Psp124BI GAGCTC 3 cut(s) 9, 207, 1491
Psp5II RGGWCCY 2 cut(s) 46, 240
Psp6I CCWGG 2 cut(s) 1498, 1683
PspFI CCCAGC 3 cut(s) 389, 988, 1247
PspGI CCWGG 2 cut(s) 1498, 1683
PspN4I GGNNCC 7 cut(s) 48, 241, 242, 457, 626, 870, 1011
PspPI GGNCC 3 cut(s) 46, 145, 240
PspPPI RGGWCCY 2 cut(s) 46, 240
PspXI VCTCGAGB 1 cut(s) 434
PstNI CAGNNNCTG 2 cut(s) 1247, 1292
PsuI RGATCY 3 cut(s) 868, 1360, 1699
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 6 cut(s) 78, 457, 755, 836, 1271, 1631
RsaNI GTAC 6 cut(s) 77, 456, 754, 835, 1270, 1630
RseI CAYNNNNRTG 1 cut(s) 1322
SacI GAGCTC 3 cut(s) 9, 207, 1491
SapI GCTCTTC 1 cut(s) 1479
SaqAI TTAA 4 cut(s) 347, 1199, 1356, 1470
Sau3AI GATC 6 cut(s) 450, 868, 1360, 1624, 1680, 1699
Sau96I GGNCC 3 cut(s) 46, 145, 240
ScaI AGTACT 1 cut(s) 755
SchI GAGTC 5 cut(s) 808, 931, 1008, 1057, 1213
ScrFI CCNGG 3 cut(s) 305, 1500, 1685
SduI GDGCHC 4 cut(s) 9, 207, 1491, 1498
SfaNI GCATC 8 cut(s) 361, 384, 1086, 1234, 1265, 1498, 1512, 1525
Sfr274I CTCGAG 1 cut(s) 434
SfuI TTCGAA 1 cut(s) 442
SgrAI CRCCGGYG 1 cut(s) 117
SinI GGWCC 2 cut(s) 46, 240
SlaI CTCGAG 1 cut(s) 434
SmiMI CAYNNNNRTG 1 cut(s) 1322
SmlI CTYRAG 5 cut(s) 263, 434, 734, 1310, 1469
SmoI CTYRAG 5 cut(s) 263, 434, 734, 1310, 1469
SphI GCATGC 1 cut(s) 1515
Sse9I AATT 6 cut(s) 554, 715, 766, 1568, 1606, 1640
SseBI AGGCCT 3 cut(s) 332, 820, 1129
SspI AATATT 1 cut(s) 1459
SspMI CTAG 2 cut(s) 1125, 1723
SstI GAGCTC 3 cut(s) 9, 207, 1491
StuI AGGCCT 3 cut(s) 332, 820, 1129
StyD4I CCNGG 3 cut(s) 303, 1498, 1683
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 3 cut(s) 76, 311, 1181
TaiI ACGT 1 cut(s) 987
TaqI TCGA 6 cut(s) 272, 428, 435, 442, 630, 1593
TasI AATT 6 cut(s) 554, 715, 766, 1568, 1606, 1640
TatI WGTACW 4 cut(s) 753, 834, 1269, 1629
TauI GCSGC 4 cut(s) 146, 1309, 1334, 1710
TfiI GAWTC 3 cut(s) 274, 682, 1452
Tru1I TTAA 4 cut(s) 347, 1199, 1356, 1470
Tru9I TTAA 4 cut(s) 347, 1199, 1356, 1470
TscAI CASTG 3 cut(s) 79, 469, 909
TseFI GTSAC 2 cut(s) 113, 1175
TseI GCWGC 8 cut(s) 234, 539, 542, 652, 844, 1328, 1343, 1710
Tsp45I GTSAC 2 cut(s) 113, 1175
TspDTI ATGAA 7 cut(s) 512, 674, 692, 953, 1395, 1581, 1588
TspRI CASTG 3 cut(s) 79, 469, 909
Vha464I CTTAAG 1 cut(s) 1469
VpaK11BI GGWCC 2 cut(s) 46, 240
XapI RAATTY 3 cut(s) 554, 766, 1568
XceI RCATGY 2 cut(s) 1056, 1515
XhoI CTCGAG 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 1192
XspI CTAG 2 cut(s) 1125, 1723
ZrmI AGTACT 1 cut(s) 755
Zsp2I ATGCAT 1 cut(s) 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.