AT3G18970

Pentatricopeptide repeat domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016670
Physical Location & Seq
Reverse (-)
6543318 .. 6545432
2115 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G18970.1

Sequence Viewer

Length: 1419 bp
ATGAGCTCTGTTTTTCCAGGACCAAGATTTTTGTCCTTGTTGCAACAGAATTCGAAAACCCTAATTCAAGCTAAGCAAATCCATGCTCAATTAGTCATCAATGGCTGCCATGATAATTCTCTATTTGGCAAACTCATAGGCCATTACTGTTCAAAGCCATCAACCGAAAGCAGCTCAAAGCTTGCTCATTTACTAGTGTTTCCTCGATTTGGTCATCCTGATAAGTTTCTCTTCAACACATTGCTTAAATGTAGCAAACCAGAGGACTCGATTCGGATTTTTGCCAATTATGCATCAAAAAGTTCGTTGCTTTATCTCAATGAGCGCACTTTTGTCTTCGTCCTTGGCGCTTGTGCTAGGTCAGCTTCGTCGTCGGCGTTACGGGTTGGTAGAATTGTTCATGGGATGGTGAAAAAGCTTGGCTTTCTATATGAATCTGAATTGATTGGAACCACTTTACTCCATTTCTACGCTAAGAATGGAGATTTACGTTATGCAAGGAAGGTGTTTGACGAAATGCCTGAGAGAACCTCTGTTACATGGAACGCAATGATAGGTGGATATTGTTCACATAAGGATAAAGGCAATCACAATGCGAGAAAGGCTATGGTTTTGTTTAGGAGATTCTCTTGCTGTGGTAGTGGAGTTAGACCTACTGATACAACCATGGTTTGTGTTCTTTCAGCCATTTCTCAGACGGGTTTGCTCGAAATTGGTTCTCTCGTTCATGGTTACATAGAAAAGCTCGGGTTCACACCCGAAGTTGATGTCTTTATCGGTACTGCACTTGTCGACATGTACTCAAAATGCGGCTGCTTGAACAACGCATTCTCCGTGTTCGAGCTAATGAAAGTGAAAAATGTCTTTACATGGACTTCAATGGCAACGGGGCTAGCTCTCAACGGTAGAGGAAACGAAACACCGAATCTTCTAAACCGAATGGCGGAATCTGGGATCAAACCAAACGAAATAACTTTCACTAGCTTGCTTTCGGCTTATCGTCATATAGGTCTTGTTGAAGAAGGAATCGAGCTGTTCAAGAGCATGAAGACAAGATTTGGTGTCACACCGGTTATCGAACACTATGGTTGCATAGTAGATCTTCTTGGTAAGGCAGGGCGGATACAAGAGGCTTATCAGTTTATATTAGCTATGCCTATAAAGCCTGACGCTATCTTGTTAAGAAGCCTCTGCAATGCCTGCAGCATTTATGGAGAGACTGTAATGGGTGAAGAGATCGGAAAGGCTCTTCTCGAGATAGAACGAGAGGACGAGAAGCTTTCGGGTTCTGAGTGCGAAGATTATGTCGCTTTATCGAATGTGTTAGCTCATAAAGGGAAATGGGTAGAGGTAGAGAAGCTGAGGAAGGAGATGAAAGAAAGAAGAATCAAAACAAGACCTGGTTACAGTTTTGTTTAA

Protein Analysis

472

Amino Acids

52.56

Weight (kDa)

9.04

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 286 - 333 1e-08 PPR repeat family
E_motif PF20431 409 - 472 2.2e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016670)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18970
fragaria_vesca FvH4_6g02930
malus_domestica MD04G1224500.v1.1 MD12G1241200.v1.1
prunus_persica Prupe.6G343200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451131
rosa_laevigata RLG00000025687
rosa_multiflora Rmu_sc0039775.1_g000001 Rmu_ssc0000461.1_g000009
rosa_roxburghii Rroxscaffold_6G00428600
rosa_rugosa Rorug02G0631800
rosa_samantha Rh3AG034600 Rh3DG034600
rosa_wichuraiana Rw3G002520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 792
AciI CCGC 3 cut(s) 810, 944, 1120
AclWI GGATC 1 cut(s) 962
AcsI RAATTY 1 cut(s) 49
AfaI GTAC 2 cut(s) 781, 800
AfiI CCNNNNNNNGG 2 cut(s) 209, 943
AflIII ACRYGT 1 cut(s) 795
AgeI ACCGGT 1 cut(s) 1069
AgsI TTSAA 7 cut(s) 68, 153, 235, 820, 879, 1019, 1039
AhlI ACTAGT 1 cut(s) 193
AjnI CCWGG 2 cut(s) 16, 1399
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 1211
AlwI GGATC 1 cut(s) 962
Ama87I CYCGRG 2 cut(s) 746, 1253
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 4 cut(s) 105, 171, 813, 1203
ApoI RAATTY 1 cut(s) 49
ArsI GACNNNNNNTTYG 4 cut(s) 753, 785, 1290, 1322
AsiGI ACCGGT 1 cut(s) 1069
AspLEI GCGC 2 cut(s) 327, 350
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 2 cut(s) 421, 1241
AsuII TTCGAA 1 cut(s) 53
AsuNHI GCTAGC 1 cut(s) 892
AvaI CYCGRG 2 cut(s) 746, 1253
AvaII GGWCC 1 cut(s) 20
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 2 cut(s) 328, 1055
Bbv12I GWGCWC 1 cut(s) 8
BbvCI CCTCAGC 1 cut(s) 1361
BbvI GCAGC 4 cut(s) 92, 183, 800, 1215
BccI CCATC 2 cut(s) 166, 400
BciT130I CCWGG 2 cut(s) 18, 1401
BciVI GTATCC 1 cut(s) 1116
BcoDI GTCTC 1 cut(s) 1211
BcuI ACTAGT 1 cut(s) 193
BfaI CTAG 4 cut(s) 194, 357, 893, 981
BfmI CTRYAG 1 cut(s) 1201
BfoI RGCGCY 1 cut(s) 351
BfuI GTATCC 1 cut(s) 1116
BglII AGATCT 1 cut(s) 1099
BisI GCNGC 5 cut(s) 106, 172, 811, 814, 1204
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 5 cut(s) 107, 173, 812, 815, 1205
Bme1390I CCNGG 2 cut(s) 18, 1401
Bme18I GGWCC 1 cut(s) 20
BmeT110I CYCGRG 2 cut(s) 746, 1253
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 1 cut(s) 451
BmrFI CCNGG 2 cut(s) 18, 1401
BmsI GCATC 1 cut(s) 302
BmtI GCTAGC 1 cut(s) 896
BpiI GAAGAC 2 cut(s) 328, 1055
BplI GAGNNNNNCTC 2 cut(s) 515, 547
Bpu10I CCTNAGC 1 cut(s) 1361
Bpu1102I GCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 343, 666
BsaWI WCCGGW 1 cut(s) 1069
BsaXI ACNNNNNCTCC 4 cut(s) 815, 845, 1206, 1236
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 943
Bse118I RCCGGY 1 cut(s) 1069
Bse3DI GCAATG 3 cut(s) 239, 555, 1201
BseBI CCWGG 2 cut(s) 18, 1401
BseDI CCNNGG 2 cut(s) 343, 666
BseGI GGATG 2 cut(s) 214, 411
BseLI CCNNNNNNNGG 2 cut(s) 209, 943
BseMI GCAATG 3 cut(s) 239, 555, 1201
BseMII CTCAG 4 cut(s) 513, 707, 1281, 1352
BseXI GCAGC 4 cut(s) 92, 183, 800, 1215
BsgI GTGCAG 1 cut(s) 768
BshFI GGCC 1 cut(s) 141
BshTI ACCGGT 1 cut(s) 1069
BsiHKAI GWGCWC 1 cut(s) 8
BsiHKCI CYCGRG 2 cut(s) 746, 1253
BsiSI CCGG 1 cut(s) 1070
BslI CCNNNNNNNGG 2 cut(s) 209, 943
BsmAI GTCTC 1 cut(s) 1211
BsmI GAATGC 1 cut(s) 827
BsnI GGCC 1 cut(s) 141
BsoBI CYCGRG 2 cut(s) 746, 1253
Bsp119I TTCGAA 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 3 cut(s) 954, 1099, 1236
Bsp1720I GCTNAGC 1 cut(s) 72
Bsp19I CCATGG 1 cut(s) 666
BspACI CCGC 3 cut(s) 810, 944, 1120
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 4 cut(s) 514, 706, 1282, 1353
BspLI GGNNCC 1 cut(s) 451
BspMAI CTGCAG 1 cut(s) 1205
BspOI GCTAGC 1 cut(s) 896
BspPI GGATC 1 cut(s) 962
BspQI GCTCTTC 1 cut(s) 1254
BspT104I TTCGAA 1 cut(s) 53
BsrDI GCAATG 3 cut(s) 239, 555, 1201
BsrFI RCCGGY 1 cut(s) 1069
BssAI RCCGGY 1 cut(s) 1069
BssECI CCNNGG 2 cut(s) 343, 666
BssMI GATC 3 cut(s) 954, 1099, 1236
BssT1I CCWWGG 2 cut(s) 343, 666
Bst2UI CCWGG 2 cut(s) 18, 1401
Bst4CI ACNGT 4 cut(s) 149, 905, 1222, 1409
Bst6I CTCTTC 3 cut(s) 236, 1227, 1254
BstAPI GCANNNNNTGC 1 cut(s) 1200
BstBI TTCGAA 1 cut(s) 53
BstC8I GCNNGC 4 cut(s) 183, 894, 986, 1201
BstDEI CTNAG 6 cut(s) 72, 474, 522, 693, 1290, 1361
BstDSI CCRYGG 1 cut(s) 666
BstF5I GGATG 2 cut(s) 214, 411
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 2 cut(s) 327, 350
BstKTI GATC 3 cut(s) 957, 1102, 1239
BstMAI GTCTC 1 cut(s) 1211
BstMBI GATC 3 cut(s) 954, 1099, 1236
BstMWI GCNNNNNNNGC 5 cut(s) 290, 362, 602, 1162, 1200
BstNI CCWGG 2 cut(s) 18, 1401
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 2 cut(s) 16, 1399
BstSFI CTRYAG 1 cut(s) 1201
BstV1I GCAGC 4 cut(s) 92, 183, 800, 1215
BstV2I GAAGAC 2 cut(s) 328, 1055
BstX2I RGATCY 1 cut(s) 1099
BstYI RGATCY 1 cut(s) 1099
BsuI GTATCC 1 cut(s) 1116
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 666
BtsCI GGATG 2 cut(s) 214, 411
Cac8I GCNNGC 4 cut(s) 183, 894, 986, 1201
CfoI GCGC 2 cut(s) 327, 350
Cfr10I RCCGGY 1 cut(s) 1069
Cfr13I GGNCC 1 cut(s) 20
CseI GACGC 1 cut(s) 1178
CsiI ACCWGGT 1 cut(s) 1399
Csp6I GTAC 2 cut(s) 780, 799
CspAI ACCGGT 1 cut(s) 1069
CviAII CATG 9 cut(s) 83, 110, 401, 540, 667, 728, 796, 870, 1045
CviQI GTAC 2 cut(s) 780, 799
DdeI CTNAG 6 cut(s) 72, 474, 522, 693, 1290, 1361
DpnI GATC 3 cut(s) 956, 1101, 1238
DpnII GATC 3 cut(s) 954, 1099, 1236
Eam1104I CTCTTC 3 cut(s) 236, 1227, 1254
EarI CTCTTC 3 cut(s) 236, 1227, 1254
EciI GGCGGA 2 cut(s) 959, 1135
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 2 cut(s) 343, 666
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 6
Eco88I CYCGRG 2 cut(s) 746, 1253
EcoICRI GAGCTC 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 16, 1399
EcoT14I CCWWGG 2 cut(s) 343, 666
EcoT22I ATGCAT 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 8
ErhI CCWWGG 2 cut(s) 343, 666
FaeI CATG 9 cut(s) 86, 113, 404, 543, 670, 731, 799, 873, 1048
FalI AAGNNNNNCTT 4 cut(s) 215, 247, 407, 439
FatI CATG 9 cut(s) 82, 109, 400, 539, 666, 727, 795, 869, 1044
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 5 cut(s) 106, 172, 811, 814, 1204
FokI GGATG 2 cut(s) 201, 418
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 5 cut(s) 106, 172, 811, 814, 1204
FspBI CTAG 4 cut(s) 194, 357, 893, 981
GlaI GCGC 2 cut(s) 326, 349
GluI GCNGC 5 cut(s) 106, 172, 811, 814, 1204
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 1 cut(s) 141
HapII CCGG 1 cut(s) 1070
HgaI GACGC 1 cut(s) 1178
HhaI GCGC 2 cut(s) 327, 350
Hin1II CATG 9 cut(s) 86, 113, 404, 543, 670, 731, 799, 873, 1048
Hin6I GCGC 2 cut(s) 325, 348
HinP1I GCGC 2 cut(s) 325, 348
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HindIII AAGCTT 3 cut(s) 179, 416, 1277
HinfI GANTC 8 cut(s) 266, 271, 434, 624, 925, 947, 1026, 1386
HpaII CCGG 1 cut(s) 1070
HphI GGTGA 2 cut(s) 421, 1241
Hpy166II GTNNAC 3 cut(s) 569, 753, 793
Hpy188I TCNGA 5 cut(s) 276, 439, 696, 1241, 1291
Hpy188III TCNNGA 4 cut(s) 218, 1039, 1253, 1255
Hpy8I GTNNAC 3 cut(s) 569, 753, 793
Hpy99I CGWCG 2 cut(s) 373, 376
HpyAV CCTTC 3 cut(s) 496, 1016, 1360
HpyCH4III ACNGT 4 cut(s) 149, 905, 1222, 1409
HpyCH4IV ACGT 1 cut(s) 490
HpyCH4V TGCA 7 cut(s) 43, 293, 497, 785, 1092, 1194, 1203
HpyF10VI GCNNNNNNNGC 5 cut(s) 290, 362, 602, 1162, 1200
HpyF3I CTNAG 6 cut(s) 72, 474, 522, 693, 1290, 1361
HpySE526I ACGT 1 cut(s) 490
Hsp92II CATG 9 cut(s) 86, 113, 404, 543, 670, 731, 799, 873, 1048
HspAI GCGC 2 cut(s) 325, 348
Kzo9I GATC 3 cut(s) 954, 1099, 1236
LguI GCTCTTC 1 cut(s) 1254
Lsp1109I GCAGC 4 cut(s) 92, 183, 800, 1215
LweI GCATC 1 cut(s) 302
MabI ACCWGGT 1 cut(s) 1399
MaeI CTAG 4 cut(s) 194, 357, 893, 981
MaeII ACGT 1 cut(s) 490
MaeIII GTNAC 5 cut(s) 378, 535, 731, 1063, 1403
MalI GATC 3 cut(s) 956, 1101, 1238
MboI GATC 3 cut(s) 954, 1099, 1236
MflI RGATCY 1 cut(s) 1099
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 8 cut(s) 49, 63, 89, 115, 286, 393, 440, 711
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 9 cut(s) 213, 256, 541, 902, 1123, 1199, 1261, 1342, 1356
Mph1103I ATGCAT 1 cut(s) 295
MseI TTAA 3 cut(s) 246, 1181, 1417
MspI CCGG 1 cut(s) 1070
MspR9I CCNGG 2 cut(s) 18, 1401
Mva1269I GAATGC 1 cut(s) 827
MvaI CCWGG 2 cut(s) 18, 1401
MwoI GCNNNNNNNGC 5 cut(s) 290, 362, 602, 1162, 1200
NcoI CCATGG 1 cut(s) 666
NdeII GATC 3 cut(s) 954, 1099, 1236
NheI GCTAGC 1 cut(s) 892
NlaIII CATG 9 cut(s) 86, 113, 404, 543, 670, 731, 799, 873, 1048
NlaIV GGNNCC 1 cut(s) 451
NmuCI GTSAC 1 cut(s) 1063
NsiI ATGCAT 1 cut(s) 295
NspI RCATGY 1 cut(s) 799
NspV TTCGAA 1 cut(s) 53
PaeR7I CTCGAG 1 cut(s) 1253
PciI ACATGT 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 1254
PcsI WCGNNNNNNNCGW 2 cut(s) 374, 831
PctI GAATGC 1 cut(s) 827
PfeI GAWTC 7 cut(s) 271, 434, 624, 925, 947, 1026, 1386
PfoI TCCNGGA 1 cut(s) 16
PinAI ACCGGT 1 cut(s) 1069
PkrI GCNGC 5 cut(s) 107, 173, 812, 815, 1205
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PscI ACATGT 1 cut(s) 795
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 2 cut(s) 16, 1399
PspGI CCWGG 2 cut(s) 16, 1399
PspN4I GGNNCC 1 cut(s) 451
PspPI GGNCC 1 cut(s) 20
PsrI GAACNNNNNNTAC 2 cut(s) 520, 552
PstI CTGCAG 1 cut(s) 1205
PsuI RGATCY 1 cut(s) 1099
RsaI GTAC 2 cut(s) 781, 800
RsaNI GTAC 2 cut(s) 780, 799
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 1 cut(s) 791
SapI GCTCTTC 1 cut(s) 1254
SaqAI TTAA 3 cut(s) 246, 1181, 1417
SatI GCNGC 5 cut(s) 106, 172, 811, 814, 1204
Sau3AI GATC 3 cut(s) 954, 1099, 1236
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 2 cut(s) 18, 1401
SduI GDGCHC 1 cut(s) 8
SexAI ACCWGGT 1 cut(s) 1399
SfaNI GCATC 1 cut(s) 302
SfcI CTRYAG 1 cut(s) 1201
Sfr274I CTCGAG 1 cut(s) 1253
SfuI TTCGAA 1 cut(s) 53
SinI GGWCC 1 cut(s) 20
SlaI CTCGAG 1 cut(s) 1253
SmlI CTYRAG 1 cut(s) 1253
SmoI CTYRAG 1 cut(s) 1253
SpeI ACTAGT 1 cut(s) 193
Sse9I AATT 8 cut(s) 49, 63, 89, 115, 286, 393, 440, 711
SsiI CCGC 3 cut(s) 810, 944, 1120
SspMI CTAG 4 cut(s) 194, 357, 893, 981
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 16, 1399
StyI CCWWGG 2 cut(s) 343, 666
TaaI ACNGT 4 cut(s) 149, 905, 1222, 1409
TaiI ACGT 1 cut(s) 493
TasI AATT 8 cut(s) 49, 63, 89, 115, 286, 393, 440, 711
TatI WGTACW 1 cut(s) 798
TauI GCSGC 1 cut(s) 813
TfiI GAWTC 7 cut(s) 271, 434, 624, 925, 947, 1026, 1386
Tru1I TTAA 3 cut(s) 246, 1181, 1417
Tru9I TTAA 3 cut(s) 246, 1181, 1417
TseFI GTSAC 1 cut(s) 1063
TseI GCWGC 4 cut(s) 105, 171, 813, 1203
Tsp45I GTSAC 1 cut(s) 1063
TspDTI ATGAA 6 cut(s) 389, 447, 716, 863, 1061, 1388
TspGWI ACGGA 1 cut(s) 823
VpaK11BI GGWCC 1 cut(s) 20
XapI RAATTY 1 cut(s) 49
XceI RCATGY 1 cut(s) 799
XhoI CTCGAG 1 cut(s) 1253
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 4 cut(s) 194, 357, 893, 981
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.