Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0016670

Physical Location & Seq

Genomic Coordinates Reverse (-)

31307771 .. 31309283

Gene Structure 1513 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1241200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGCACCATCTCCCAAGAGCCAGAGCTCTCTCTCTCCTAAACCTAAACCTAAACCTAAAATCCACGCACCAACTCAAAAAAATCCATGCCCAATTAATCACCAATGGCCTCAAATCTCCCTCCCTCCATGCCAAACTCATCCAGCAATACTCTGCCTTATCATCCCCAAAAAGCACCAACCTTTATGCCCGTTTGGTATTCCAACACTTTCAAGAACCAAACTTGTTCCTCCTAAACACTTTGATCAGATGCTCCCAACCCAGAGATTCACTTCTTGTTTTTGCCAATTGGGTTTCTAAATCAACCTTAGTTTTTGATGATTCTACTTACATTTTTGTTCTTGGAGCTTGTGCTAGATTGCCTTCAGTTCCAACGTTGTTACTAGGCAGACAGATACATGGTCGGATTGTGAAACATGATGTCGTATCAAATATTTCGGTGCCAACAACGTTAATACATTATTATGGTAGTAATGAAGATGTTGTTTCGGCACGGAATGTGTTTGATGAAATGCCTGTGAGAAGTTGTGTTACGTGGAACGCAATGATCACGGGGTATTGTTCGCAAAGAGTGAGTGCCCGCGATGCGTTGGTGTTGTTTCGGGACATGTTGGATGATGTTTGTGGGGTGAAACCTACGGATACTACAATGGTTTGTGTTCTTTCTGCGGCGTCTCAGCTAGGTGTGTTGGAAACGGGGGCTTGTGTACATGGTTATGTGGAGAAGACAATGTGTGTTCCTGACAATGATGTGTTTATGGGGACTGGCCTTGTTGATATGTACTCGAAATGCGGATGTGTTGATAGCGCTTTCTCCGTTTTCAAGCGAATGAAAGAGAAGAATGTATTGACTTGGACAGCAATGGCGACCGGGCTAGCCATTCACGGCAAGGGAAATGAAGCATTGGAGCTTTTGGATGTAATGCAGGGTTCTGGAATAAAACCCAATGCAGTGACTTTCACCGGCTTGCTTTCGGCTTGTTGCCATTCAGGTCTCGTTGAAGAAGGCCTCCATTTGTTCCATTTGATGAAGAGCAAGTTTGATGTCGTGCCTCGGATGCAACATTATGGCTGCATTGTCGACATGCTTAGCCGCGGAGGGCACTTGAAAGAAGCCTATGAGTTTATAGTAGGGATGCCAGTTGAACCTGATGCCGTCTTGTGGAGGAGTTTGCTAAGTGCTTGCAATCTCCATGGGGATGTTGCAATGGGAGAGAAGGTGGGGAAGAAGCTGCTCTGTGTACAGTTCACACAGAGTTACGTGGATTCAACTCCAAAGAGTGAGGACTATGTAGCATTGTCGAACGTTTATGCTTTTGCTGAAAGGTGGGAGGATGTGGAGATGGTAAGACAGGAAATGAAGGTTAAGGGAATCGAAAACAAAGCCGGTTGGAGTTCGGTTCAAACTTCAAGCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

473

Amino Acids

52.23

Weight (kDa)

7.88

Isoelectric Point (pI)

33.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	176 - 223	4.4e-06	PPR repeat family
PPR_2	PF13041	280 - 329	1e-11	PPR repeat family
PPR_1	PF12854	312 - 344	3.7e-06	PPR repeat
PPR	PF01535	318 - 346	1e-05	PPR repeat
E_motif	PF20431	427 - 468	4.1e-10	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18970
fragaria_vesca	FvH4_6g02930
malus_domestica	MD04G1224500.v1.1 MD12G1241200.v1.1
prunus_persica	Prupe.6G343200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0451131
rosa_laevigata	RLG00000025687
rosa_multiflora	Rmu_sc0039775.1_g000001 Rmu_ssc0000461.1_g000009
rosa_roxburghii	Rroxscaffold_6G00428600
rosa_rugosa	Rorug02G0631800
rosa_samantha	Rh3AG034600 Rh3DG034600
rosa_wichuraiana	Rw3G002520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	439
AccI	GTMKAC	1 cut(s)	1080
AccII	CGCG	2 cut(s)	582, 1095
AciI	CCGC	5 cut(s)	580, 668, 792, 1093, 1095
AclI	AACGTT	3 cut(s)	374, 449, 1305
AcuI	CTGAAG	1 cut(s)	348
AcyI	GRCGYC	1 cut(s)	671
AfaI	GTAC	3 cut(s)	708, 782, 1242
AfeI	AGCGCT	1 cut(s)	808
AfiI	CCNNNNNNNGG	1 cut(s)	1161
AflIII	ACRYGT	1 cut(s)	606
AgsI	TTSAA	8 cut(s)	212, 823, 1001, 1108, 1145, 1269, 1403, 1410
AluBI	AGCT	5 cut(s)	26, 347, 679, 910, 1231
AluI	AGCT	5 cut(s)	26, 347, 679, 910, 1231
Alw21I	GWGCWC	1 cut(s)	28
Alw26I	GTCTC	2 cut(s)	678, 998
Aor51HI	AGCGCT	1 cut(s)	808
AoxI	GGCC	3 cut(s)	106, 766, 1006
ApeKI	GCWGC	2 cut(s)	1071, 1231
AseI	ATTAAT	1 cut(s)	95
AspLEI	GCGC	1 cut(s)	809
AsuC2I	CCSGG	1 cut(s)	871
AsuHPI	GGTGA	3 cut(s)	91, 640, 952
AsuNHI	GCTAGC	1 cut(s)	874
BaeGI	GKGCMC	2 cut(s)	580, 1104
BanI	GGYRCC	1 cut(s)	439
BanII	GRGCYC	1 cut(s)	28
BarI	GAAGNNNNNNTAC	2 cut(s)	514, 546
BbsI	GAAGAC	1 cut(s)	731
Bbv12I	GWGCWC	1 cut(s)	28
BbvI	GCAGC	2 cut(s)	1058, 1218
BccI	CCATC	2 cut(s)	15, 1336
BceAI	ACGGC	2 cut(s)	901, 1139
BciVI	GTATCC	1 cut(s)	634
BclI	TGATCA	2 cut(s)	243, 546
BcnI	CCSGG	1 cut(s)	871
BcoDI	GTCTC	2 cut(s)	678, 998
BfaI	CTAG	4 cut(s)	354, 383, 680, 875
BfoI	RGCGCY	1 cut(s)	810
BfuI	GTATCC	1 cut(s)	634
BisI	GCNGC	4 cut(s)	669, 1072, 1093, 1232
BlpI	GCTNAGC	1 cut(s)	1088
BlsI	GCNGC	4 cut(s)	670, 1073, 1094, 1233
Bme1390I	CCNGG	1 cut(s)	871
BmiI	GGNNCC	1 cut(s)	441
BmrFI	CCNGG	1 cut(s)	871
BmsI	GCATC	5 cut(s)	239, 574, 1047, 1125, 1141
BmtI	GCTAGC	1 cut(s)	878
BpiI	GAAGAC	1 cut(s)	731
Bpu1102I	GCTNAGC	1 cut(s)	1088
BpuMI	CCSGG	1 cut(s)	871
BsaAI	YACGTR	2 cut(s)	534, 1261
BsaHI	GRCGYC	1 cut(s)	671
BsaI	GGTCTC	1 cut(s)	998
BsaJI	CCNNGG	3 cut(s)	1052, 1093, 1192
BsaXI	ACNNNNNCTCC	2 cut(s)	1203, 1233
Bsc4I	CCNNNNNNNGG	1 cut(s)	1161
Bse118I	RCCGGY	2 cut(s)	962, 1385
Bse1I	ACTGG	2 cut(s)	769, 1139
Bse3DI	GCAATG	3 cut(s)	549, 867, 1212
BseDI	CCNNGG	3 cut(s)	1052, 1093, 1192
BseGI	GGATG	9 cut(s)	138, 161, 619, 800, 922, 1062, 1140, 1204, 1339
BseLI	CCNNNNNNNGG	1 cut(s)	1161
BseMI	GCAATG	3 cut(s)	549, 867, 1212
BseMII	CTCAG	1 cut(s)	689
BseNI	ACTGG	2 cut(s)	769, 1139
BseRI	GAGGAG	1 cut(s)	1180
BseSI	GKGCMC	2 cut(s)	580, 1104
BseXI	GCAGC	2 cut(s)	1058, 1218
Bsh1236I	CGCG	2 cut(s)	582, 1095
Bsh1285I	CGRYCG	1 cut(s)	870
BshFI	GGCC	3 cut(s)	108, 768, 1008
BshNI	GGYRCC	1 cut(s)	439
BsiEI	CGRYCG	1 cut(s)	870
BsiHKAI	GWGCWC	1 cut(s)	28
BsiSI	CCGG	3 cut(s)	870, 963, 1386
BslFI	GGGAC	2 cut(s)	617, 775
BslI	CCNNNNNNNGG	1 cut(s)	1161
BsmAI	GTCTC	2 cut(s)	678, 998
BsmBI	CGTCTC	1 cut(s)	678
BsmFI	GGGAC	2 cut(s)	617, 775
BsnI	GGCC	3 cut(s)	108, 768, 1008
Bso31I	GGTCTC	1 cut(s)	998
Bsp1286I	GDGCHC	3 cut(s)	28, 580, 1104
Bsp1407I	TGTACA	2 cut(s)	706, 1240
Bsp143I	GATC	2 cut(s)	243, 546
Bsp1720I	GCTNAGC	1 cut(s)	1088
Bsp19I	CCATGG	1 cut(s)	1192
BspACI	CCGC	5 cut(s)	580, 668, 792, 1093, 1095
BspANI	GGCC	3 cut(s)	108, 768, 1008
BspCNI	CTCAG	1 cut(s)	688
BspFNI	CGCG	2 cut(s)	582, 1095
BspLI	GGNNCC	1 cut(s)	441
BspOI	GCTAGC	1 cut(s)	878
BspQI	GCTCTTC	1 cut(s)	1025
BspT107I	GGYRCC	1 cut(s)	439
BspTNI	GGTCTC	1 cut(s)	998
BsrDI	GCAATG	3 cut(s)	549, 867, 1212
BsrFI	RCCGGY	2 cut(s)	962, 1385
BsrGI	TGTACA	2 cut(s)	706, 1240
BsrI	ACTGG	2 cut(s)	769, 1139
BssAI	RCCGGY	2 cut(s)	962, 1385
BssECI	CCNNGG	3 cut(s)	1052, 1093, 1192
BssMI	GATC	2 cut(s)	243, 546
BssNI	GRCGYC	1 cut(s)	671
BssT1I	CCWWGG	1 cut(s)	1192
Bst4CI	ACNGT	1 cut(s)	1245
Bst6I	CTCTTC	1 cut(s)	1025
BstACI	GRCGYC	1 cut(s)	671
BstAUI	TGTACA	2 cut(s)	706, 1240
BstBAI	YACGTR	2 cut(s)	534, 1261
BstC8I	GCNNGC	4 cut(s)	580, 876, 968, 1183
BstDEI	CTNAG	4 cut(s)	307, 675, 1088, 1175
BstDSI	CCRYGG	2 cut(s)	1093, 1192
BstF5I	GGATG	9 cut(s)	138, 161, 619, 800, 922, 1062, 1140, 1204, 1339
BstFNI	CGCG	2 cut(s)	582, 1095
BstH2I	RGCGCY	1 cut(s)	810
BstHHI	GCGC	1 cut(s)	809
BstKTI	GATC	2 cut(s)	246, 549
BstMAI	GTCTC	2 cut(s)	678, 998
BstMBI	GATC	2 cut(s)	243, 546
BstMCI	CGRYCG	1 cut(s)	870
BstMWI	GCNNNNNNNGC	2 cut(s)	584, 1057
BstNSI	RCATGY	2 cut(s)	610, 1087
BstSCI	CCNGG	1 cut(s)	869
BstSLI	GKGCMC	2 cut(s)	580, 1104
BstUI	CGCG	2 cut(s)	582, 1095
BstV1I	GCAGC	2 cut(s)	1058, 1218
BstV2I	GAAGAC	1 cut(s)	731
BsuI	GTATCC	1 cut(s)	634
BsuRI	GGCC	3 cut(s)	108, 768, 1008
BtgI	CCRYGG	2 cut(s)	1093, 1192
BtgZI	GCGATG	1 cut(s)	597
BtsCI	GGATG	9 cut(s)	138, 161, 619, 800, 922, 1062, 1140, 1204, 1339
BtsI	GCAGTG	1 cut(s)	957
BtsIMutI	CAGTG	1 cut(s)	957
Cac8I	GCNNGC	4 cut(s)	580, 876, 968, 1183
CfoI	GCGC	1 cut(s)	809
Cfr10I	RCCGGY	2 cut(s)	962, 1385
Cfr42I	CCGCGG	1 cut(s)	1096
CseI	GACGC	1 cut(s)	660
Csp6I	GTAC	3 cut(s)	707, 781, 1241
CviAII	CATG	8 cut(s)	86, 128, 398, 416, 607, 710, 1084, 1193
CviQI	GTAC	3 cut(s)	707, 781, 1241
DdeI	CTNAG	4 cut(s)	307, 675, 1088, 1175
DpnI	GATC	2 cut(s)	245, 548
DpnII	GATC	2 cut(s)	243, 546
Eam1104I	CTCTTC	1 cut(s)	1025
EarI	CTCTTC	1 cut(s)	1025
Ecl136II	GAGCTC	1 cut(s)	26
Eco130I	CCWWGG	1 cut(s)	1192
Eco147I	AGGCCT	1 cut(s)	1008
Eco24I	GRGCYC	1 cut(s)	28
Eco31I	GGTCTC	1 cut(s)	998
Eco47III	AGCGCT	1 cut(s)	808
Eco53kI	GAGCTC	1 cut(s)	26
Eco57I	CTGAAG	1 cut(s)	348
EcoICRI	GAGCTC	1 cut(s)	26
EcoT14I	CCWWGG	1 cut(s)	1192
EcoT38I	GRGCYC	1 cut(s)	28
ErhI	CCWWGG	1 cut(s)	1192
Esp3I	CGTCTC	1 cut(s)	678
FaeI	CATG	8 cut(s)	89, 131, 401, 419, 610, 713, 1087, 1196
FaqI	GGGAC	2 cut(s)	617, 775
FatI	CATG	8 cut(s)	85, 127, 397, 415, 606, 709, 1083, 1192
FauI	CCCGC	1 cut(s)	587
FbaI	TGATCA	2 cut(s)	243, 546
FblI	GTMKAC	1 cut(s)	1080
Fnu4HI	GCNGC	4 cut(s)	669, 1072, 1093, 1232
FokI	GGATG	9 cut(s)	125, 148, 626, 807, 929, 1069, 1147, 1211, 1346
FriOI	GRGCYC	1 cut(s)	28
Fsp4HI	GCNGC	4 cut(s)	669, 1072, 1093, 1232
FspBI	CTAG	4 cut(s)	354, 383, 680, 875
GlaI	GCGC	1 cut(s)	808
GluI	GCNGC	4 cut(s)	669, 1072, 1093, 1232
HaeII	RGCGCY	1 cut(s)	810
HaeIII	GGCC	3 cut(s)	108, 768, 1008
HapII	CCGG	3 cut(s)	870, 963, 1386
HgaI	GACGC	1 cut(s)	660
HhaI	GCGC	1 cut(s)	809
Hin1I	GRCGYC	1 cut(s)	671
Hin1II	CATG	8 cut(s)	89, 131, 401, 419, 610, 713, 1087, 1196
Hin6I	GCGC	1 cut(s)	807
HinP1I	GCGC	1 cut(s)	807
HincII	GTYRAC	1 cut(s)	1081
HindII	GTYRAC	1 cut(s)	1081
HinfI	GANTC	4 cut(s)	266, 320, 1265, 1371
HpaII	CCGG	3 cut(s)	870, 963, 1386
HphI	GGTGA	3 cut(s)	91, 640, 952
Hpy166II	GTNNAC	4 cut(s)	707, 1081, 1241, 1248
Hpy188I	TCNGA	3 cut(s)	248, 405, 1056
Hpy188III	TCNNGA	4 cut(s)	212, 602, 740, 933
Hpy8I	GTNNAC	4 cut(s)	707, 1081, 1241, 1248
HpyAV	CCTTC	4 cut(s)	372, 998, 1210, 1354
HpyCH4III	ACNGT	1 cut(s)	1245
HpyCH4IV	ACGT	5 cut(s)	374, 449, 533, 1260, 1305
HpyCH4V	TGCA	7 cut(s)	4, 925, 950, 1060, 1074, 1185, 1205
HpyF10VI	GCNNNNNNNGC	2 cut(s)	584, 1057
HpyF3I	CTNAG	4 cut(s)	307, 675, 1088, 1175
HpySE526I	ACGT	5 cut(s)	374, 449, 533, 1260, 1305
Hsp92I	GRCGYC	1 cut(s)	671
Hsp92II	CATG	8 cut(s)	89, 131, 401, 419, 610, 713, 1087, 1196
HspAI	GCGC	1 cut(s)	807
Ksp22I	TGATCA	2 cut(s)	243, 546
KspI	CCGCGG	1 cut(s)	1096
Kzo9I	GATC	2 cut(s)	243, 546
LguI	GCTCTTC	1 cut(s)	1025
LmnI	GCTCC	3 cut(s)	257, 344, 907
Lsp1109I	GCAGC	2 cut(s)	1058, 1218
LweI	GCATC	5 cut(s)	239, 574, 1047, 1125, 1141
MaeI	CTAG	4 cut(s)	354, 383, 680, 875
MaeII	ACGT	5 cut(s)	374, 449, 533, 1260, 1305
MaeIII	GTNAC	4 cut(s)	378, 529, 952, 1256
MalI	GATC	2 cut(s)	245, 548
MboI	GATC	2 cut(s)	243, 546
MboII	GAAGA	6 cut(s)	488, 736, 850, 1013, 1042, 1237
MfeI	CAATTG	1 cut(s)	286
MhlI	GDGCHC	3 cut(s)	28, 580, 1104
MluCI	AATT	2 cut(s)	92, 286
MmeI	TCCRAC	6 cut(s)	226, 383, 395, 591, 669, 1370
MseI	TTAA	3 cut(s)	95, 452, 1365
MslI	CAYNNNNRTG	3 cut(s)	462, 714, 1197
MspA1I	CMGCKG	1 cut(s)	1095
MspI	CCGG	3 cut(s)	870, 963, 1386
MspR9I	CCNGG	1 cut(s)	871
MunI	CAATTG	1 cut(s)	286
MvnI	CGCG	2 cut(s)	582, 1095
MwoI	GCNNNNNNNGC	2 cut(s)	584, 1057
NciI	CCSGG	1 cut(s)	871
NcoI	CCATGG	1 cut(s)	1192
NdeII	GATC	2 cut(s)	243, 546
NheI	GCTAGC	1 cut(s)	874
NlaIII	CATG	8 cut(s)	89, 131, 401, 419, 610, 713, 1087, 1196
NlaIV	GGNNCC	1 cut(s)	441
NmuCI	GTSAC	1 cut(s)	952
NspI	RCATGY	2 cut(s)	610, 1087
PceI	AGGCCT	1 cut(s)	1008
PciI	ACATGT	1 cut(s)	606
PciSI	GCTCTTC	1 cut(s)	1025
PfeI	GAWTC	4 cut(s)	266, 320, 1265, 1371
PkrI	GCNGC	4 cut(s)	670, 1073, 1094, 1233
Ppu21I	YACGTR	2 cut(s)	534, 1261
PscI	ACATGT	1 cut(s)	606
PshBI	ATTAAT	1 cut(s)	95
Psp124BI	GAGCTC	1 cut(s)	28
Psp1406I	AACGTT	3 cut(s)	374, 449, 1305
PspN4I	GGNNCC	1 cut(s)	441
RsaI	GTAC	3 cut(s)	708, 782, 1242
RsaNI	GTAC	3 cut(s)	707, 781, 1241
RseI	CAYNNNNRTG	3 cut(s)	462, 714, 1197
SacI	GAGCTC	1 cut(s)	28
SacII	CCGCGG	1 cut(s)	1096
SalI	GTCGAC	1 cut(s)	1079
SapI	GCTCTTC	1 cut(s)	1025
SaqAI	TTAA	3 cut(s)	95, 452, 1365
SatI	GCNGC	4 cut(s)	669, 1072, 1093, 1232
Sau3AI	GATC	2 cut(s)	243, 546
ScrFI	CCNGG	1 cut(s)	871
SduI	GDGCHC	3 cut(s)	28, 580, 1104
SfaNI	GCATC	5 cut(s)	239, 574, 1047, 1125, 1141
Sfr303I	CCGCGG	1 cut(s)	1096
SgrBI	CCGCGG	1 cut(s)	1096
SmiMI	CAYNNNNRTG	3 cut(s)	462, 714, 1197
Sse9I	AATT	2 cut(s)	92, 286
SseBI	AGGCCT	1 cut(s)	1008
SsiI	CCGC	5 cut(s)	580, 668, 792, 1093, 1095
SspI	AATATT	1 cut(s)	433
SspMI	CTAG	4 cut(s)	354, 383, 680, 875
SstI	GAGCTC	1 cut(s)	28
StuI	AGGCCT	1 cut(s)	1008
StyD4I	CCNGG	1 cut(s)	869
StyI	CCWWGG	1 cut(s)	1192
TaaI	ACNGT	1 cut(s)	1245
TaiI	ACGT	5 cut(s)	377, 452, 536, 1263, 1308
TaqI	TCGA	4 cut(s)	785, 1080, 1301, 1374
TasI	AATT	2 cut(s)	92, 286
TatI	WGTACW	3 cut(s)	706, 780, 1240
TauI	GCSGC	2 cut(s)	671, 1095
TfiI	GAWTC	4 cut(s)	266, 320, 1265, 1371
Tru1I	TTAA	3 cut(s)	95, 452, 1365
Tru9I	TTAA	3 cut(s)	95, 452, 1365
TscAI	CASTG	1 cut(s)	957
TseFI	GTSAC	1 cut(s)	952
TseI	GCWGC	2 cut(s)	1071, 1231
Tsp45I	GTSAC	1 cut(s)	952
TspDTI	ATGAA	6 cut(s)	489, 522, 845, 912, 1043, 1373
TspGWI	ACGGA	3 cut(s)	508, 653, 805
TspRI	CASTG	1 cut(s)	957
VspI	ATTAAT	1 cut(s)	95
XceI	RCATGY	2 cut(s)	610, 1087
XmiI	GTMKAC	1 cut(s)	1080
XspI	CTAG	4 cut(s)	354, 383, 680, 875

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.