AT3G25545

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016649
Physical Location & Seq
Forward (+)
9276845 .. 9278691
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25545.1

Sequence Viewer

Length: 804 bp
ATGGAAGATGTACTTACCGAGAATCCTCCACCTTCAAGATTCTTCCAAGAAGATCTTAACAACTTTGTTCAGCCACCTGAATCCCTTCCATCTCCTTTCATCATATTCTCAAACCCGAAACCTGAACTTCCTCTCCGACCATCTCTACTTATCATAGCCATATCTTCACCTTCCCTCTATATTTTCCATAGTTTACCTTCAAAGACTCTCCTTGGAAGTCTCATCATGCCTGAAGTTCCGTTTTCTGGAAACACCATGGAACCTTCTTTAGAAGACAAATCCTGCAACATATATTCCTTGAGTAGTGCTGATGATAAAAACTTGACCCTCCTCGTCTCTGTTCAGCTTCCTGTATCTCCTGAACGGTCTAACCTAGTCTCGAGATTGCTCATTGCCAAAGACATTATCCCCGAGCGGGTGATAATCTTGGATTCCATTCAGAGCCGTAACTTCAGAGGTAGACTCTCACCAGACGAAACATTGGCAGCCAAACTCGAGACATCTGCTGAGAAGAAAGCAACAACTCACACCGGTTTAGTGAATCTCGATTACTTCCCATCAGGGAGTGTAATCGACGGTCTGAGTGCTTCACTTTTGAGCCGGTGCCAGCTAAAGAACATAAGAGGAACATTGGTGATCTCATGGCCTGAGTTTGGTCCTTCAGTGATAAGATTCGCCGGAGCTCTGCTGAAGAAAATTGTCCCGAGTTTGGATGTTGCGAGTGTGAACAAAGATCTGGAGAAGAATTCTTCAAGAACTGGTCTTAAAAAGGATACTTGGCTTGACTCTGATCTATATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC1

Protein Analysis

267

Amino Acids

29.39

Weight (kDa)

5.55

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016649)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25545
fragaria_vesca FvH4_4g28700
malus_domestica MD13G1053100.v1.1 MD16G1052100.v1.1
rosa_chinensis RchiOBHm_Chr4g0436821
rosa_laevigata RLG00000006494
rosa_multiflora Rmu_sc0001837.1_g000014
rosa_roxburghii Rroxscaffold_5G00377890
rosa_rugosa Rorug04G0296500
rosa_samantha Rh4AG350800 Rh4BG359800 Rh4CG374100 Rh4DG353700
rosa_wichuraiana Rw4G030700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 603
AccBSI CCGCTC 1 cut(s) 415
AccI GTMKAC 1 cut(s) 460
AciI CCGC 1 cut(s) 415
AcsI RAATTY 1 cut(s) 745
AcuI CTGAAG 4 cut(s) 252, 436, 645, 710
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 4 cut(s) 245, 415, 653, 709
AgeI ACCGGT 1 cut(s) 530
AgsI TTSAA 3 cut(s) 36, 201, 753
AloI GAACNNNNNNTCC 2 cut(s) 117, 149
AluBI AGCT 3 cut(s) 346, 610, 683
AluI AGCT 3 cut(s) 346, 610, 683
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 4 cut(s) 224, 340, 382, 491
Ama87I CYCGRG 4 cut(s) 379, 410, 494, 703
AoxI GGCC 1 cut(s) 644
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 1 cut(s) 745
AsiGI ACCGGT 1 cut(s) 530
Asp700I GAANNNNTTC 1 cut(s) 84
AspS9I GGNCC 1 cut(s) 656
AsuHPI GGTGA 4 cut(s) 159, 430, 459, 646
AvaI CYCGRG 4 cut(s) 379, 410, 494, 703
AvaII GGWCC 1 cut(s) 656
BanI GGYRCC 1 cut(s) 603
BanII GRGCYC 1 cut(s) 685
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 1 cut(s) 497
BccI CCATC 3 cut(s) 97, 148, 565
BceAI ACGGC 1 cut(s) 429
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BciVI GTATCC 1 cut(s) 766
BcoDI GTCTC 4 cut(s) 224, 340, 382, 491
BfaI CTAG 1 cut(s) 374
BfuI GTATCC 1 cut(s) 766
BglII AGATCT 2 cut(s) 52, 733
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
Bme18I GGWCC 1 cut(s) 656
BmeT110I CYCGRG 4 cut(s) 379, 410, 494, 703
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 2 cut(s) 261, 605
BpiI GAAGAC 1 cut(s) 279
BplI GAGNNNNNCTC 2 cut(s) 447, 479
BpmI CTGGAG 1 cut(s) 758
BpuEI CTTGAG 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 211, 255
BsaWI WCCGGW 1 cut(s) 530
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 415, 653, 709
Bse118I RCCGGY 2 cut(s) 530, 600
Bse1I ACTGG 1 cut(s) 763
Bse3DI GCAATG 1 cut(s) 390
BseDI CCNNGG 2 cut(s) 211, 255
BseGI GGATG 1 cut(s) 718
BseLI CCNNNNNNNGG 4 cut(s) 245, 415, 653, 709
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 3 cut(s) 498, 572, 639
BseNI ACTGG 1 cut(s) 763
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 1 cut(s) 497
BshFI GGCC 1 cut(s) 646
BshNI GGYRCC 1 cut(s) 603
BshTI ACCGGT 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 685
BsiHKCI CYCGRG 4 cut(s) 379, 410, 494, 703
BsiSI CCGG 3 cut(s) 531, 601, 678
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 4 cut(s) 245, 415, 653, 709
BsmAI GTCTC 4 cut(s) 224, 340, 382, 491
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 1 cut(s) 686
BsnI GGCC 1 cut(s) 646
BsoBI CYCGRG 4 cut(s) 379, 410, 494, 703
Bsp1286I GDGCHC 1 cut(s) 685
Bsp143I GATC 4 cut(s) 52, 636, 733, 790
Bsp19I CCATGG 1 cut(s) 255
BspACI CCGC 1 cut(s) 415
BspANI GGCC 1 cut(s) 646
BspCNI CTCAG 3 cut(s) 499, 573, 640
BspLI GGNNCC 2 cut(s) 261, 605
BspT107I GGYRCC 1 cut(s) 603
BsrBI CCGCTC 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 2 cut(s) 530, 600
BsrI ACTGG 1 cut(s) 763
BssAI RCCGGY 2 cut(s) 530, 600
BssECI CCNNGG 2 cut(s) 211, 255
BssMI GATC 4 cut(s) 52, 636, 733, 790
BssT1I CCWWGG 2 cut(s) 211, 255
Bst4CI ACNGT 2 cut(s) 366, 578
BstC8I GCNNGC 1 cut(s) 608
BstDEI CTNAG 3 cut(s) 507, 581, 648
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 1 cut(s) 718
BstKTI GATC 4 cut(s) 55, 639, 736, 793
BstMAI GTCTC 4 cut(s) 224, 340, 382, 491
BstMBI GATC 4 cut(s) 52, 636, 733, 790
BstV1I GCAGC 1 cut(s) 497
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 2 cut(s) 52, 733
BstYI RGATCY 2 cut(s) 52, 733
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 1 cut(s) 646
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 1 cut(s) 718
BtsIMutI CAGTG 1 cut(s) 669
Cac8I GCNNGC 1 cut(s) 608
Cfr10I RCCGGY 2 cut(s) 530, 600
Cfr13I GGNCC 1 cut(s) 656
Csp6I GTAC 1 cut(s) 11
CspAI ACCGGT 1 cut(s) 530
CviAII CATG 4 cut(s) 226, 256, 642, 801
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 3 cut(s) 507, 581, 648
DpnI GATC 4 cut(s) 54, 638, 735, 792
DpnII GATC 4 cut(s) 52, 636, 733, 790
Ecl136II GAGCTC 1 cut(s) 683
Eco130I CCWWGG 2 cut(s) 211, 255
Eco24I GRGCYC 1 cut(s) 685
Eco47I GGWCC 1 cut(s) 656
Eco53kI GAGCTC 1 cut(s) 683
Eco57I CTGAAG 4 cut(s) 252, 436, 645, 710
Eco88I CYCGRG 4 cut(s) 379, 410, 494, 703
EcoICRI GAGCTC 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 745
EcoT14I CCWWGG 2 cut(s) 211, 255
EcoT38I GRGCYC 1 cut(s) 685
ErhI CCWWGG 2 cut(s) 211, 255
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 4 cut(s) 229, 259, 645, 804
FalI AAGNNNNNCTT 3 cut(s) 29, 39, 71
FaqI GGGAC 1 cut(s) 686
FatI CATG 4 cut(s) 225, 255, 641, 800
FauI CCCGC 1 cut(s) 408
FblI GTMKAC 1 cut(s) 460
Fnu4HI GCNGC 1 cut(s) 486
FokI GGATG 1 cut(s) 725
FriOI GRGCYC 1 cut(s) 685
Fsp4HI GCNGC 1 cut(s) 486
FspBI CTAG 1 cut(s) 374
GluI GCNGC 1 cut(s) 486
GsuI CTGGAG 1 cut(s) 758
HaeIII GGCC 1 cut(s) 646
HapII CCGG 3 cut(s) 531, 601, 678
Hin1II CATG 4 cut(s) 229, 259, 645, 804
HinfI GANTC 9 cut(s) 22, 39, 80, 205, 431, 462, 541, 672, 785
HpaII CCGG 3 cut(s) 531, 601, 678
HphI GGTGA 4 cut(s) 159, 430, 459, 646
Hpy166II GTNNAC 3 cut(s) 194, 461, 727
Hpy188I TCNGA 5 cut(s) 137, 441, 455, 582, 790
Hpy8I GTNNAC 3 cut(s) 194, 461, 727
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 6 cut(s) 42, 95, 180, 207, 273, 669
HpyCH4III ACNGT 2 cut(s) 366, 578
HpyCH4V TGCA 1 cut(s) 285
HpyF3I CTNAG 3 cut(s) 507, 581, 648
Hsp92II CATG 4 cut(s) 229, 259, 645, 804
Kzo9I GATC 4 cut(s) 52, 636, 733, 790
LmnI GCTCC 1 cut(s) 680
Lsp1109I GCAGC 1 cut(s) 497
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 446
MalI GATC 4 cut(s) 54, 638, 735, 792
MbiI CCGCTC 1 cut(s) 415
MboI GATC 4 cut(s) 52, 636, 733, 790
MboII GAAGA 9 cut(s) 17, 34, 62, 156, 284, 523, 703, 741, 754
MflI RGATCY 2 cut(s) 52, 733
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 2 cut(s) 696, 745
MlyI GAGTC 3 cut(s) 199, 456, 779
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 7 cut(s) 36, 141, 185, 338, 341, 449, 617
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 2 cut(s) 57, 765
MspI CCGG 3 cut(s) 531, 601, 678
NcoI CCATGG 1 cut(s) 255
NdeII GATC 4 cut(s) 52, 636, 733, 790
NlaIII CATG 4 cut(s) 229, 259, 645, 804
NlaIV GGNNCC 2 cut(s) 261, 605
PaeR7I CTCGAG 2 cut(s) 379, 494
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 6 cut(s) 22, 39, 80, 431, 541, 672
PinAI ACCGGT 1 cut(s) 530
PkrI GCNGC 1 cut(s) 487
PleI GAGTC 3 cut(s) 199, 456, 779
PpsI GAGTC 3 cut(s) 199, 456, 779
Psp124BI GAGCTC 1 cut(s) 685
PspN4I GGNNCC 2 cut(s) 261, 605
PspPI GGNCC 1 cut(s) 656
PsuI RGATCY 2 cut(s) 52, 733
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
SacI GAGCTC 1 cut(s) 685
SaqAI TTAA 2 cut(s) 57, 765
SatI GCNGC 1 cut(s) 486
Sau3AI GATC 4 cut(s) 52, 636, 733, 790
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 3 cut(s) 199, 456, 779
SduI GDGCHC 1 cut(s) 685
Sfr274I CTCGAG 2 cut(s) 379, 494
SinI GGWCC 1 cut(s) 656
SlaI CTCGAG 2 cut(s) 379, 494
SmlI CTYRAG 3 cut(s) 298, 379, 494
SmoI CTYRAG 3 cut(s) 298, 379, 494
Sse9I AATT 2 cut(s) 696, 745
SsiI CCGC 1 cut(s) 415
SspMI CTAG 1 cut(s) 374
SstI GAGCTC 1 cut(s) 685
StyI CCWWGG 2 cut(s) 211, 255
TaaI ACNGT 2 cut(s) 366, 578
TaqI TCGA 4 cut(s) 380, 495, 546, 573
TasI AATT 2 cut(s) 696, 745
TatI WGTACW 1 cut(s) 10
TfiI GAWTC 6 cut(s) 22, 39, 80, 431, 541, 672
Tru1I TTAA 2 cut(s) 57, 765
Tru9I TTAA 2 cut(s) 57, 765
TscAI CASTG 1 cut(s) 669
TseI GCWGC 1 cut(s) 485
TspDTI ATGAA 1 cut(s) 88
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 1 cut(s) 669
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 1 cut(s) 745
XhoI CTCGAG 2 cut(s) 379, 494
XmiI GTMKAC 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.