MD13G1053100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016649
Physical Location & Seq
Reverse (-)
3760817 .. 3761620
804 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1053100.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGAAGATGTACTGACTGAGATTCCGCCACCATCGAGGTTCTTCCAGGAAGATCTCAACAGCTTCACACCTCCGTCGCCGCCCCTTCCCTCTCCCTTCCTCCTGTTCTCTCAAAACCCTGACGGAGCGGCTGCAGAGTTCCCTATCCGCCCCTCTCTCCTCATCCTCGCCCTATCCTCCCCATCCCTTCACCTCTTCCACAGCGTCTCCTCCAAATCCCTAATCGGAACCCTAATCCTCCCGGAAACCCCCTTCTCCGGCAACTCAATCGACCCCTCTCTCACAGACAAGTCCTGCAACATCTACTCCCTCCCGAACCCCCAAAACCCTGAATCCCCAACCCTTCTCGTCTCCTTCCAGTGCCCAATCGCCGCCGAGAGATGCCACGCCGTGGCCAAGCTGCTCACTTCTCATCAGATCGTCCCCCAGAGGATCCTGATTCTCGACTCGGTCCAGACCCTGAATTTCCGCGGCAAGCTCTCGAAGGACGAGGCGGCGGCGTTCAAGCTGGAGACCACCGAGGAAAGAAAGGCGCCGTCTTTGAAGCTGGAGTATTTCCCGTCGGGCAGCGTCGTGGATGGGCTGGGCGCGGCGCTGTTGGCGGCGTGCGAAATCAAGAACATCAGGGCGGCGCTCTGCGTGACGTGGCCGCAATTCGGAGCTCCGGTGCTGTCCCTGATCGGGTCCCACATCTTCCGTGCCTTTGATTTGGAACTGAGCATTGATTCTGATTGCAAGGATTATGTGATTCTAGGGCAAGATAAGGATCACCATCATCCTTTTGATTCTGATTTGTATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC1

Protein Analysis

268

Amino Acids

29.16

Weight (kDa)

5.1

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016649)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25545
fragaria_vesca FvH4_4g28700
malus_domestica MD13G1053100.v1.1 MD16G1052100.v1.1
rosa_chinensis RchiOBHm_Chr4g0436821
rosa_laevigata RLG00000006494
rosa_multiflora Rmu_sc0001837.1_g000014
rosa_roxburghii Rroxscaffold_5G00377890
rosa_rugosa Rorug04G0296500
rosa_samantha Rh4AG350800 Rh4BG359800 Rh4CG374100 Rh4DG353700
rosa_wichuraiana Rw4G030700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 532
AccB7I CCANNNNNTGG 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 128
AccI GTMKAC 1 cut(s) 797
AccII CGCG 2 cut(s) 471, 590
AclWI GGATC 3 cut(s) 427, 440, 774
AcoI YGGCCR 2 cut(s) 393, 647
AcsI RAATTY 1 cut(s) 463
AcyI GRCGYC 1 cut(s) 533
AdeI CACNNNGTG 1 cut(s) 391
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 4 cut(s) 257, 391, 656, 681
AgsI TTSAA 2 cut(s) 505, 544
AjiI CACGTC 1 cut(s) 645
AjnI CCWGG 1 cut(s) 45
AluBI AGCT 6 cut(s) 63, 400, 478, 508, 547, 662
AluI AGCT 6 cut(s) 63, 400, 478, 508, 547, 662
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 3 cut(s) 211, 355, 506
AlwI GGATC 3 cut(s) 427, 440, 774
AlwNI CAGNNNCTG 1 cut(s) 460
AoxI GGCC 2 cut(s) 393, 647
ApeKI GCWGC 3 cut(s) 131, 400, 567
ApoI RAATTY 1 cut(s) 463
AspLEI GCGC 4 cut(s) 535, 590, 595, 634
AspS9I GGNCC 2 cut(s) 451, 684
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 2 cut(s) 182, 761
AvaII GGWCC 2 cut(s) 451, 684
BaeGI GKGCMC 1 cut(s) 365
BalI TGGCCA 1 cut(s) 395
BamHI GGATCC 1 cut(s) 432
BanI GGYRCC 1 cut(s) 532
BanII GRGCYC 1 cut(s) 664
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 3 cut(s) 118, 387, 579
BccI CCATC 4 cut(s) 40, 190, 572, 780
BceAI ACGGC 2 cut(s) 374, 520
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 1 cut(s) 47
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 3 cut(s) 211, 355, 506
BfaI CTAG 1 cut(s) 752
BfmI CTRYAG 1 cut(s) 132
BfoI RGCGCY 3 cut(s) 536, 596, 635
BglII AGATCT 1 cut(s) 52
Bme1390I CCNGG 2 cut(s) 47, 242
Bme18I GGWCC 2 cut(s) 451, 684
BmgBI CACGTC 1 cut(s) 645
BmgT120I GGNCC 2 cut(s) 451, 684
BmiI GGNNCC 5 cut(s) 229, 434, 534, 685, 686
BmrFI CCNGG 2 cut(s) 47, 242
BmsI GCATC 1 cut(s) 371
BpmI CTGGAG 2 cut(s) 530, 569
BpuMI CCSGG 1 cut(s) 242
BsaBI GATNNNNATC 2 cut(s) 765, 771
BsaHI GRCGYC 1 cut(s) 533
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 3 cut(s) 390, 469, 519
BsaWI WCCGGW 1 cut(s) 664
BsaXI ACNNNNNCTCC 6 cut(s) 191, 221, 290, 320, 651, 681
Bsc4I CCNNNNNNNGG 4 cut(s) 257, 391, 656, 681
Bse1I ACTGG 1 cut(s) 358
Bse8I GATNNNNATC 2 cut(s) 765, 771
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 3 cut(s) 390, 469, 519
BseGI GGATG 4 cut(s) 162, 182, 583, 775
BseJI GATNNNNATC 2 cut(s) 765, 771
BseLI CCNNNNNNNGG 4 cut(s) 257, 391, 656, 681
BseMII CTCAG 2 cut(s) 9, 707
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 2 cut(s) 149, 199
BseSI GKGCMC 1 cut(s) 365
BseXI GCAGC 3 cut(s) 118, 387, 579
BseYI CCCAGC 1 cut(s) 583
Bsh1236I CGCG 2 cut(s) 471, 590
BshFI GGCC 2 cut(s) 395, 649
BshNI GGYRCC 1 cut(s) 532
BsiHKAI GWGCWC 1 cut(s) 664
BsiSI CCGG 3 cut(s) 242, 258, 665
BslFI GGGAC 3 cut(s) 407, 658, 670
BslI CCNNNNNNNGG 4 cut(s) 257, 391, 656, 681
BsmAI GTCTC 3 cut(s) 211, 355, 506
BsmBI CGTCTC 2 cut(s) 211, 355
BsmFI GGGAC 3 cut(s) 407, 658, 670
BsnI GGCC 2 cut(s) 395, 649
Bso31I GGTCTC 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 365, 664
Bsp143I GATC 5 cut(s) 52, 417, 432, 678, 766
BspANI GGCC 2 cut(s) 395, 649
BspCNI CTCAG 2 cut(s) 10, 708
BspFNI CGCG 2 cut(s) 471, 590
BspLI GGNNCC 5 cut(s) 229, 434, 534, 685, 686
BspMAI CTGCAG 1 cut(s) 136
BspPI GGATC 3 cut(s) 427, 440, 774
BspT107I GGYRCC 1 cut(s) 532
BspTNI GGTCTC 1 cut(s) 506
BsrBI CCGCTC 1 cut(s) 128
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 3 cut(s) 390, 469, 519
BssMI GATC 5 cut(s) 52, 417, 432, 678, 766
BssNAI GTATAC 1 cut(s) 798
BssNI GRCGYC 1 cut(s) 533
Bst1107I GTATAC 1 cut(s) 798
Bst2UI CCWGG 1 cut(s) 47
Bst6I CTCTTC 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 533
BstC8I GCNNGC 2 cut(s) 476, 607
BstDEI CTNAG 2 cut(s) 18, 716
BstDSI CCRYGG 2 cut(s) 390, 469
BstF5I GGATG 4 cut(s) 162, 182, 583, 775
BstFNI CGCG 2 cut(s) 471, 590
BstH2I RGCGCY 3 cut(s) 536, 596, 635
BstHHI GCGC 4 cut(s) 535, 590, 595, 634
BstKTI GATC 5 cut(s) 55, 420, 435, 681, 769
BstMAI GTCTC 3 cut(s) 211, 355, 506
BstMBI GATC 5 cut(s) 52, 417, 432, 678, 766
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNI CCWGG 1 cut(s) 47
BstSCI CCNGG 2 cut(s) 45, 240
BstSFI CTRYAG 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 365
BstUI CGCG 2 cut(s) 471, 590
BstV1I GCAGC 3 cut(s) 118, 387, 579
BstX2I RGATCY 2 cut(s) 52, 432
BstYI RGATCY 2 cut(s) 52, 432
BstZ17I GTATAC 1 cut(s) 798
BsuRI GGCC 2 cut(s) 395, 649
BtgI CCRYGG 2 cut(s) 390, 469
BtrI CACGTC 1 cut(s) 645
BtsCI GGATG 4 cut(s) 162, 182, 583, 775
BtsIMutI CAGTG 1 cut(s) 365
Cac8I GCNNGC 2 cut(s) 476, 607
CaiI CAGNNNCTG 1 cut(s) 460
CfoI GCGC 4 cut(s) 535, 590, 595, 634
Cfr13I GGNCC 2 cut(s) 451, 684
Cfr42I CCGCGG 1 cut(s) 472
CseI GACGC 2 cut(s) 193, 559
Csp6I GTAC 1 cut(s) 11
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 2 cut(s) 18, 716
DinI GGCGCC 1 cut(s) 534
DpnI GATC 5 cut(s) 54, 419, 434, 680, 768
DpnII GATC 5 cut(s) 52, 417, 432, 678, 766
DraIII CACNNNGTG 1 cut(s) 391
EaeI YGGCCR 2 cut(s) 393, 647
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
EciI GGCGGA 2 cut(s) 15, 137
Ecl136II GAGCTC 1 cut(s) 662
Eco24I GRGCYC 1 cut(s) 664
Eco31I GGTCTC 1 cut(s) 506
Eco47I GGWCC 2 cut(s) 451, 684
Eco53kI GAGCTC 1 cut(s) 662
EcoICRI GAGCTC 1 cut(s) 662
EcoO109I RGGNCCY 1 cut(s) 684
EcoRII CCWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 664
EgeI GGCGCC 1 cut(s) 534
EheI GGCGCC 1 cut(s) 534
Esp3I CGTCTC 2 cut(s) 211, 355
FaiI YATR 2 cut(s) 744, 798
FaqI GGGAC 3 cut(s) 407, 658, 670
FblI GTMKAC 1 cut(s) 797
FokI GGATG 4 cut(s) 149, 169, 590, 762
FriOI GRGCYC 1 cut(s) 664
FspBI CTAG 1 cut(s) 752
GlaI GCGC 4 cut(s) 534, 589, 594, 633
GsaI CCCAGC 1 cut(s) 587
GsuI CTGGAG 2 cut(s) 530, 569
HaeII RGCGCY 3 cut(s) 536, 596, 635
HaeIII GGCC 2 cut(s) 395, 649
HapII CCGG 3 cut(s) 242, 258, 665
HgaI GACGC 2 cut(s) 193, 559
HhaI GCGC 4 cut(s) 535, 590, 595, 634
Hin1I GRCGYC 1 cut(s) 533
Hin6I GCGC 4 cut(s) 533, 588, 593, 632
HinP1I GCGC 4 cut(s) 533, 588, 593, 632
HinfI GANTC 7 cut(s) 22, 332, 439, 446, 725, 748, 785
HpaII CCGG 3 cut(s) 242, 258, 665
HphI GGTGA 2 cut(s) 182, 761
Hpy166II GTNNAC 1 cut(s) 798
Hpy188I TCNGA 5 cut(s) 227, 417, 659, 730, 790
Hpy188III TCNNGA 6 cut(s) 313, 436, 443, 454, 481, 616
Hpy8I GTNNAC 1 cut(s) 798
Hpy99I CGWCG 3 cut(s) 79, 565, 575
HpyAV CCTTC 7 cut(s) 95, 106, 197, 262, 353, 364, 478
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 3 cut(s) 134, 297, 735
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpyF3I CTNAG 2 cut(s) 18, 716
HpySE526I ACGT 1 cut(s) 644
Hsp92I GRCGYC 1 cut(s) 533
HspAI GCGC 4 cut(s) 533, 588, 593, 632
KasI GGCGCC 1 cut(s) 532
KflI GGGWCCC 1 cut(s) 684
KspI CCGCGG 1 cut(s) 472
Kzo9I GATC 5 cut(s) 52, 417, 432, 678, 766
LmnI GCTCC 3 cut(s) 125, 659, 667
Lsp1109I GCAGC 3 cut(s) 118, 387, 579
LweI GCATC 1 cut(s) 371
MaeI CTAG 1 cut(s) 752
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 1 cut(s) 640
MalI GATC 5 cut(s) 54, 419, 434, 680, 768
MbiI CCGCTC 1 cut(s) 128
MboI GATC 5 cut(s) 52, 417, 432, 678, 766
MboII GAAGA 5 cut(s) 17, 34, 62, 187, 685
MflI RGATCY 2 cut(s) 52, 432
MhlI GDGCHC 2 cut(s) 365, 664
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 2 cut(s) 463, 653
MluNI TGGCCA 1 cut(s) 395
Mly113I GGCGCC 1 cut(s) 533
MlyI GAGTC 1 cut(s) 440
Mox20I TGGCCA 1 cut(s) 395
MscI TGGCCA 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 471
MspI CCGG 3 cut(s) 242, 258, 665
MspR9I CCNGG 2 cut(s) 47, 242
MteI GCGCNGCGC 1 cut(s) 591
MvaI CCWGG 1 cut(s) 47
MvnI CGCG 2 cut(s) 471, 590
MwoI GCNNNNNNNGC 1 cut(s) 599
NarI GGCGCC 1 cut(s) 533
NciI CCSGG 1 cut(s) 242
NdeII GATC 5 cut(s) 52, 417, 432, 678, 766
NlaIV GGNNCC 5 cut(s) 229, 434, 534, 685, 686
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 1 cut(s) 640
PfeI GAWTC 6 cut(s) 22, 332, 439, 725, 748, 785
PflFI GACNNNGTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 391
PfoI TCCNGGA 2 cut(s) 45, 240
PleI GAGTC 1 cut(s) 440
PluTI GGCGCC 1 cut(s) 536
PpsI GAGTC 1 cut(s) 440
PpuMI RGGWCCY 1 cut(s) 684
Psp124BI GAGCTC 1 cut(s) 664
Psp5II RGGWCCY 1 cut(s) 684
Psp6I CCWGG 1 cut(s) 45
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 5 cut(s) 229, 434, 534, 685, 686
PspPI GGNCC 2 cut(s) 451, 684
PspPPI RGGWCCY 1 cut(s) 684
PstI CTGCAG 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 460
PsuI RGATCY 2 cut(s) 52, 432
PsyI GACNNNGTC 1 cut(s) 449
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
SacI GAGCTC 1 cut(s) 664
SacII CCGCGG 1 cut(s) 472
Sau3AI GATC 5 cut(s) 52, 417, 432, 678, 766
Sau96I GGNCC 2 cut(s) 451, 684
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 2 cut(s) 47, 242
SduI GDGCHC 2 cut(s) 365, 664
SfaNI GCATC 1 cut(s) 371
SfcI CTRYAG 1 cut(s) 132
SfoI GGCGCC 1 cut(s) 534
Sfr303I CCGCGG 1 cut(s) 472
SgrBI CCGCGG 1 cut(s) 472
SinI GGWCC 2 cut(s) 451, 684
Sse9I AATT 2 cut(s) 463, 653
SspDI GGCGCC 1 cut(s) 532
SspMI CTAG 1 cut(s) 752
SstI GAGCTC 1 cut(s) 664
StyD4I CCNGG 2 cut(s) 45, 240
TaiI ACGT 1 cut(s) 647
TaqI TCGA 4 cut(s) 35, 270, 444, 482
TaqII GACCGA 1 cut(s) 439
TasI AATT 2 cut(s) 463, 653
TatI WGTACW 1 cut(s) 10
TfiI GAWTC 6 cut(s) 22, 332, 439, 725, 748, 785
TscAI CASTG 1 cut(s) 365
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 3 cut(s) 131, 400, 567
Tsp45I GTSAC 1 cut(s) 640
TspGWI ACGGA 3 cut(s) 63, 138, 686
TspRI CASTG 1 cut(s) 365
Tth111I GACNNNGTC 1 cut(s) 449
Van91I CCANNNNNTGG 1 cut(s) 391
VpaK11BI GGWCC 2 cut(s) 451, 684
XapI RAATTY 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 797
XspI CTAG 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.