Rh4AG350800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016649
Physical Location & Seq
Reverse (-)
65919647 .. 65921297
1651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG350800.1

Sequence Viewer

Length: 780 bp
ATGGAAGACGTCTTGACGGAGGTTCCGCCACCTTCTCGGTTCTTCCAGGAAGATCTGAACAACTTCACGCCTCCGTCACCGCCCATTCCCTCTCCTTTCCTTGTTTTCCCAACCGCCGCCGACCTCGACCTTCCCCTCCGCCCCTCTCTCCTCATCATCGCCATCTCCTCCCCCTCCCTCCACCTCTTCCACACCGCGGCCTCCAAAACCCTCATCGGAACCCTAATCCTCCCGGAAACCTCCTTCGCCGGAAACTCGGTCCAGCCCTCTCTCAAGGACAAGTCCTGCAACCTCTACTCCCTCGACGGCGACCGCCAGACCCTCCTCGTCTCGGTCCAGTGCCCTATCGCCGCCGATCGGTGCCACGCCGTCGCCGAGCTGCTGGTTTCTCAGCACATCGTTCCGGAGAGGGTTCTGATCCTGGATTCCGTTCAGAATCGGAACTTCCGGGGCAAGCTTTCCAGGGACGAGGCGCTGGCGTTCAAGCTCGAAACAACGTCGGAGAGGAAATCGAAATCGAAATCTTTGAAATTGGAGTATTTGCCTTCGGCGAGTGTGGTGGATGGGTTGGGTGCTGCTCTGTTGGCCAAGTGTGAAATCAACAAGATCAAGGCGGTGCTTTGTGTCACCTGGCCGGAGTTTGGTGCCGCCGCGCTGGAGCTGATCAAGTTTAGGGTTTTGCCTGCTCTGGATTTGGGTCTGAGCTTCAAGTCTGATGCTGATGATTATGGAAGTTTGGGACAAGATAAGGATCCTTTTGATTCTGATTTGTATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC1

Protein Analysis

259

Amino Acids

28.21

Weight (kDa)

5.16

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAC1 PF16094 88 - 208 3.5e-06 Proteasome assembly chaperone 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016649)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25545
fragaria_vesca FvH4_4g28700
malus_domestica MD13G1053100.v1.1 MD16G1052100.v1.1
rosa_chinensis RchiOBHm_Chr4g0436821
rosa_laevigata RLG00000006494
rosa_multiflora Rmu_sc0001837.1_g000014
rosa_roxburghii Rroxscaffold_5G00377890
rosa_rugosa Rorug04G0296500
rosa_samantha Rh4AG350800 Rh4BG359800 Rh4CG374100 Rh4DG353700
rosa_wichuraiana Rw4G030700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 12
AccB1I GGYRCC 2 cut(s) 360, 644
AccII CGCG 2 cut(s) 197, 653
AccIII TCCGGA 1 cut(s) 403
AclWI GGATC 3 cut(s) 412, 746, 759
AcoI YGGCCR 2 cut(s) 585, 632
AcyI GRCGYC 1 cut(s) 9
AfiI CCNNNNNNNGG 4 cut(s) 196, 331, 357, 641
AgsI TTSAA 3 cut(s) 484, 529, 709
AjnI CCWGG 4 cut(s) 45, 420, 461, 629
AluBI AGCT 5 cut(s) 379, 457, 487, 661, 705
AluI AGCT 5 cut(s) 379, 457, 487, 661, 705
Alw26I GTCTC 1 cut(s) 334
AlwI GGATC 3 cut(s) 412, 746, 759
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 3 cut(s) 198, 585, 632
ApeKI GCWGC 2 cut(s) 379, 575
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 2 cut(s) 475, 655
AspS9I GGNCC 2 cut(s) 259, 334
AsuC2I CCSGG 2 cut(s) 233, 449
AsuHPI GGTGA 2 cut(s) 69, 619
AvaII GGWCC 2 cut(s) 259, 334
BaeGI GKGCMC 1 cut(s) 344
BalI TGGCCA 1 cut(s) 587
BamHI GGATCC 1 cut(s) 751
BanI GGYRCC 2 cut(s) 360, 644
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 366, 562
BccI CCATC 2 cut(s) 170, 557
BceAI ACGGC 2 cut(s) 322, 353
BciT130I CCWGG 4 cut(s) 47, 422, 463, 631
BclI TGATCA 1 cut(s) 663
BcnI CCSGG 2 cut(s) 233, 449
BcoDI GTCTC 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 476
BglII AGATCT 1 cut(s) 52
BisI GCNGC 7 cut(s) 117, 198, 351, 380, 576, 648, 651
BlsI GCNGC 7 cut(s) 118, 199, 352, 381, 577, 649, 652
Bme1390I CCNGG 6 cut(s) 47, 233, 422, 449, 463, 631
Bme18I GGWCC 2 cut(s) 259, 334
BmgT120I GGNCC 2 cut(s) 259, 334
BmiI GGNNCC 5 cut(s) 24, 220, 362, 646, 753
BmrFI CCNGG 6 cut(s) 47, 233, 422, 449, 463, 631
BmsI GCATC 1 cut(s) 706
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 677
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 2 cut(s) 233, 449
BsaBI GATNNNNATC 1 cut(s) 750
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 195, 448, 462
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 331, 357, 641
Bse1I ACTGG 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 750
BseAI TCCGGA 1 cut(s) 403
BseBI CCWGG 4 cut(s) 47, 422, 463, 631
BseDI CCNNGG 3 cut(s) 195, 448, 462
BseGI GGATG 1 cut(s) 568
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 4 cut(s) 196, 331, 357, 641
BseMII CTCAG 2 cut(s) 404, 692
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 3 cut(s) 140, 157, 314
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 2 cut(s) 366, 562
Bsh1236I CGCG 2 cut(s) 197, 653
Bsh1285I CGRYCG 2 cut(s) 313, 358
BshFI GGCC 3 cut(s) 200, 587, 634
BshNI GGYRCC 2 cut(s) 360, 644
BsiEI CGRYCG 2 cut(s) 313, 358
BsiSI CCGG 5 cut(s) 233, 249, 404, 448, 635
BslFI GGGAC 2 cut(s) 479, 753
BslI CCNNNNNNNGG 4 cut(s) 196, 331, 357, 641
BsmAI GTCTC 1 cut(s) 334
BsmBI CGTCTC 1 cut(s) 334
BsmFI GGGAC 2 cut(s) 479, 753
BsnI GGCC 3 cut(s) 200, 587, 634
Bsp1286I GDGCHC 1 cut(s) 344
Bsp13I TCCGGA 1 cut(s) 403
Bsp143I GATC 6 cut(s) 52, 355, 417, 606, 663, 751
BspANI GGCC 3 cut(s) 200, 587, 634
BspCNI CTCAG 2 cut(s) 403, 693
BspEI TCCGGA 1 cut(s) 403
BspFNI CGCG 2 cut(s) 197, 653
BspLI GGNNCC 5 cut(s) 24, 220, 362, 646, 753
BspPI GGATC 3 cut(s) 412, 746, 759
BspT107I GGYRCC 2 cut(s) 360, 644
BsrI ACTGG 1 cut(s) 337
BssECI CCNNGG 3 cut(s) 195, 448, 462
BssMI GATC 6 cut(s) 52, 355, 417, 606, 663, 751
BssNI GRCGYC 1 cut(s) 9
Bst2UI CCWGG 4 cut(s) 47, 422, 463, 631
Bst6I CTCTTC 1 cut(s) 191
BstACI GRCGYC 1 cut(s) 9
BstC8I GCNNGC 3 cut(s) 455, 477, 684
BstDEI CTNAG 2 cut(s) 390, 701
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 568
BstFNI CGCG 2 cut(s) 197, 653
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 2 cut(s) 475, 655
BstKTI GATC 6 cut(s) 55, 358, 420, 609, 666, 754
BstMAI GTCTC 1 cut(s) 334
BstMBI GATC 6 cut(s) 52, 355, 417, 606, 663, 751
BstMCI CGRYCG 2 cut(s) 313, 358
BstMWI GCNNNNNNNGC 1 cut(s) 584
BstNI CCWGG 4 cut(s) 47, 422, 463, 631
BstSCI CCNGG 6 cut(s) 45, 231, 420, 447, 461, 629
BstSLI GKGCMC 1 cut(s) 344
BstUI CGCG 2 cut(s) 197, 653
BstV1I GCAGC 2 cut(s) 366, 562
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 2 cut(s) 52, 751
BstYI RGATCY 2 cut(s) 52, 751
BsuRI GGCC 3 cut(s) 200, 587, 634
BtgI CCRYGG 1 cut(s) 195
BtgZI GCGATG 1 cut(s) 142
BtsCI GGATG 1 cut(s) 568
BtsIMutI CAGTG 1 cut(s) 344
Cac8I GCNNGC 3 cut(s) 455, 477, 684
CfoI GCGC 2 cut(s) 475, 655
Cfr13I GGNCC 2 cut(s) 259, 334
Cfr42I CCGCGG 1 cut(s) 198
CviJI RGCY 9 cut(s) 200, 265, 379, 457, 487, 587, 634, 661, 705
CviKI_1 RGCY 9 cut(s) 200, 265, 379, 457, 487, 587, 634, 661, 705
DdeI CTNAG 2 cut(s) 390, 701
DpnI GATC 6 cut(s) 54, 357, 419, 608, 665, 753
DpnII GATC 6 cut(s) 52, 355, 417, 606, 663, 751
EaeI YGGCCR 2 cut(s) 585, 632
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
EciI GGCGGA 2 cut(s) 15, 128
Eco47I GGWCC 2 cut(s) 259, 334
EcoRII CCWGG 4 cut(s) 45, 420, 461, 629
Esp3I CGTCTC 1 cut(s) 334
FaiI YATR 1 cut(s) 729
FaqI GGGAC 2 cut(s) 479, 753
FbaI TGATCA 1 cut(s) 663
Fnu4HI GCNGC 7 cut(s) 117, 198, 351, 380, 576, 648, 651
FokI GGATG 1 cut(s) 575
Fsp4HI GCNGC 7 cut(s) 117, 198, 351, 380, 576, 648, 651
GlaI GCGC 2 cut(s) 474, 654
GluI GCNGC 7 cut(s) 117, 198, 351, 380, 576, 648, 651
GsuI CTGGAG 1 cut(s) 677
HaeII RGCGCY 1 cut(s) 476
HaeIII GGCC 3 cut(s) 200, 587, 634
HapII CCGG 5 cut(s) 233, 249, 404, 448, 635
HhaI GCGC 2 cut(s) 475, 655
Hin1I GRCGYC 1 cut(s) 9
Hin6I GCGC 2 cut(s) 473, 653
HinP1I GCGC 2 cut(s) 473, 653
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 3 cut(s) 425, 436, 761
HpaII CCGG 5 cut(s) 233, 249, 404, 448, 635
HphI GGTGA 2 cut(s) 69, 619
Hpy188I TCNGA 9 cut(s) 57, 218, 417, 435, 441, 502, 702, 715, 766
Hpy188III TCNNGA 4 cut(s) 13, 404, 689, 777
Hpy99I CGWCG 3 cut(s) 308, 374, 502
HpyAV CCTTC 4 cut(s) 42, 140, 253, 555
HpyCH4IV ACGT 2 cut(s) 9, 497
HpyCH4V TGCA 1 cut(s) 288
HpyF10VI GCNNNNNNNGC 1 cut(s) 584
HpyF3I CTNAG 2 cut(s) 390, 701
HpySE526I ACGT 2 cut(s) 9, 497
Hsp92I GRCGYC 1 cut(s) 9
HspAI GCGC 2 cut(s) 473, 653
Kpn2I TCCGGA 1 cut(s) 403
Ksp22I TGATCA 1 cut(s) 663
KspI CCGCGG 1 cut(s) 198
Kzo9I GATC 6 cut(s) 52, 355, 417, 606, 663, 751
LmnI GCTCC 1 cut(s) 658
Lsp1109I GCAGC 2 cut(s) 366, 562
LweI GCATC 1 cut(s) 706
MaeII ACGT 2 cut(s) 9, 497
MaeIII GTNAC 2 cut(s) 75, 625
MalI GATC 6 cut(s) 54, 357, 419, 608, 665, 753
MboI GATC 6 cut(s) 52, 355, 417, 606, 663, 751
MboII GAAGA 4 cut(s) 17, 34, 62, 178
MflI RGATCY 2 cut(s) 52, 751
MhlI GDGCHC 1 cut(s) 344
MlsI TGGCCA 1 cut(s) 587
MluCI AATT 1 cut(s) 530
MluNI TGGCCA 1 cut(s) 587
MmeI TCCRAC 1 cut(s) 480
Mox20I TGGCCA 1 cut(s) 587
MroI TCCGGA 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 62
MscI TGGCCA 1 cut(s) 587
Msp20I TGGCCA 1 cut(s) 587
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 5 cut(s) 233, 249, 404, 448, 635
MspR9I CCNGG 6 cut(s) 47, 233, 422, 449, 463, 631
MvaI CCWGG 4 cut(s) 47, 422, 463, 631
MvnI CGCG 2 cut(s) 197, 653
MwoI GCNNNNNNNGC 1 cut(s) 584
NciI CCSGG 2 cut(s) 233, 449
NdeII GATC 6 cut(s) 52, 355, 417, 606, 663, 751
NlaIV GGNNCC 5 cut(s) 24, 220, 362, 646, 753
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 75, 625
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 3 cut(s) 425, 436, 761
PfoI TCCNGGA 3 cut(s) 45, 231, 420
PkrI GCNGC 7 cut(s) 118, 199, 352, 381, 577, 649, 652
Ple19I CGATCG 1 cut(s) 358
Psp6I CCWGG 4 cut(s) 45, 420, 461, 629
PspGI CCWGG 4 cut(s) 45, 420, 461, 629
PspN4I GGNNCC 5 cut(s) 24, 220, 362, 646, 753
PspPI GGNCC 2 cut(s) 259, 334
PsuI RGATCY 2 cut(s) 52, 751
PvuI CGATCG 1 cut(s) 358
SacII CCGCGG 1 cut(s) 198
SatI GCNGC 7 cut(s) 117, 198, 351, 380, 576, 648, 651
Sau3AI GATC 6 cut(s) 52, 355, 417, 606, 663, 751
Sau96I GGNCC 2 cut(s) 259, 334
ScrFI CCNGG 6 cut(s) 47, 233, 422, 449, 463, 631
SduI GDGCHC 1 cut(s) 344
SfaNI GCATC 1 cut(s) 706
Sfr303I CCGCGG 1 cut(s) 198
SgrBI CCGCGG 1 cut(s) 198
SinI GGWCC 2 cut(s) 259, 334
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 1 cut(s) 530
StyD4I CCNGG 6 cut(s) 45, 231, 420, 447, 461, 629
TaiI ACGT 2 cut(s) 12, 500
TaqI TCGA 5 cut(s) 126, 303, 489, 512, 518
TaqII GACCGA 2 cut(s) 247, 322
TasI AATT 1 cut(s) 530
TauI GCSGC 5 cut(s) 119, 200, 353, 650, 653
TfiI GAWTC 3 cut(s) 425, 436, 761
TscAI CASTG 1 cut(s) 344
TseFI GTSAC 2 cut(s) 75, 625
TseI GCWGC 2 cut(s) 379, 575
Tsp45I GTSAC 2 cut(s) 75, 625
TspGWI ACGGA 3 cut(s) 32, 63, 418
TspRI CASTG 1 cut(s) 344
VpaK11BI GGWCC 2 cut(s) 259, 334
XmnI GAANNNNTTC 1 cut(s) 62
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.