MD16G1052100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016649
Physical Location & Seq
Reverse (-)
3723011 .. 3723814
804 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1052100.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGAAGATGTACTGACTGAGATTCCGCCGCCATCGAGGTTCTTTCAGGAAGATCTCAACAACTTCACACCTCCGTCACCGCCCCTTCCCTCTCCCTTCCTCTTGTTCTCTCAAAATCCTAATGGAGCGGCCGCAGAGATGCCTCTCCGCCCCTCTCTCCTCATCGTTGCCCTATCCTCCCCATCCCTCCACCTCTTCCACAACGTATCCTACAAAACCCTGATCGGAACCCTAATCCTCCCGGAAACCCCCTTCTCCGGCAACTCAATCGACCCCTCTCTCAAAGACAAGTCCTGCAACATCTACTCCCTCCCAAACCCCCAAACCCCTGAATCCCCAACCCTCCTCGTTTCCTTCCAGTGCCCAATCGGCGCTGAGAGATGCCACGCCGTGGCCAAGCTGCTCACTTCTCACCAGATCGTCCCCCAGAGGGTCCTGATTCTCGACTCGGTCCAGACCCTCAACTTCCGAGGCAGGCTTTCGAAGGACGAGGCGGCAGCGTTCAAGCTGGAGACCACCGCGGAAAGAAAGGCGCCGTCTTTGAAGCTGGAATATTTCCCCTCGGCCAGCGTCGTGGATGGGCTGGGCGCGGCGCTGTTGGCGGCGTGCGAAATCAAGAACATCAGGGCGGCGCTCTGCGTGACGTGGCCGCAACTCGGAGCTTTGGTGCTGTCCCTGATTAGGTCCCACATCCTCCCAGCCTTTGATTTGGAATCGAGCATTGATTCTGGTTGTGAGGATTATGTGATTTTAGGGCAAGATAAGGATCACGGTCATCCTTTTGATTCCGATATGTATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAC1

Protein Analysis

268

Amino Acids

29.09

Weight (kDa)

5.21

Isoelectric Point (pI)

58.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016649)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25545
fragaria_vesca FvH4_4g28700
malus_domestica MD13G1053100.v1.1 MD16G1052100.v1.1
rosa_chinensis RchiOBHm_Chr4g0436821
rosa_laevigata RLG00000006494
rosa_multiflora Rmu_sc0001837.1_g000014
rosa_roxburghii Rroxscaffold_5G00377890
rosa_rugosa Rorug04G0296500
rosa_samantha Rh4AG350800 Rh4BG359800 Rh4CG374100 Rh4DG353700
rosa_wichuraiana Rw4G030700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 532
AccB7I CCANNNNNTGG 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 128
AccI GTMKAC 1 cut(s) 797
AccII CGCG 2 cut(s) 521, 590
AclWI GGATC 1 cut(s) 774
AcoI YGGCCR 4 cut(s) 129, 393, 564, 647
AcyI GRCGYC 1 cut(s) 533
AdeI CACNNNGTG 1 cut(s) 391
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 5 cut(s) 257, 391, 430, 656, 681
AgsI TTSAA 2 cut(s) 505, 544
AjiI CACGTC 1 cut(s) 645
AluBI AGCT 4 cut(s) 400, 508, 547, 662
AluI AGCT 4 cut(s) 400, 508, 547, 662
Alw26I GTCTC 1 cut(s) 506
AlwI GGATC 1 cut(s) 774
AoxI GGCC 4 cut(s) 129, 393, 564, 647
ApeKI GCWGC 2 cut(s) 400, 497
AspLEI GCGC 5 cut(s) 374, 535, 590, 595, 634
AspS9I GGNCC 3 cut(s) 433, 451, 684
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 2 cut(s) 69, 404
AsuII TTCGAA 1 cut(s) 482
AvaII GGWCC 3 cut(s) 433, 451, 684
BaeGI GKGCMC 1 cut(s) 365
BalI TGGCCA 1 cut(s) 395
BanI GGYRCC 1 cut(s) 532
BbvI GCAGC 2 cut(s) 387, 509
BccI CCATC 3 cut(s) 40, 190, 572
BceAI ACGGC 2 cut(s) 374, 520
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciVI GTATCC 1 cut(s) 217
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 506
BfoI RGCGCY 4 cut(s) 375, 536, 596, 635
BfuI GTATCC 1 cut(s) 217
BglI GCCNNNNNGGC 1 cut(s) 369
BglII AGATCT 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 3 cut(s) 433, 451, 684
BmgBI CACGTC 1 cut(s) 645
BmgT120I GGNCC 3 cut(s) 433, 451, 684
BmiI GGNNCC 4 cut(s) 229, 434, 534, 686
BmrFI CCNGG 1 cut(s) 242
BmsI GCATC 2 cut(s) 129, 371
BpmI CTGGAG 1 cut(s) 530
Bpu14I TTCGAA 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 242
BsaBI GATNNNNATC 1 cut(s) 765
BsaHI GRCGYC 1 cut(s) 533
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 4 cut(s) 390, 469, 519, 561
BsaXI ACNNNNNCTCC 4 cut(s) 290, 320, 651, 681
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 391, 430, 656, 681
Bse1I ACTGG 1 cut(s) 358
Bse8I GATNNNNATC 1 cut(s) 765
BseDI CCNNGG 4 cut(s) 390, 469, 519, 561
BseGI GGATG 4 cut(s) 182, 583, 690, 775
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 5 cut(s) 257, 391, 430, 656, 681
BseMII CTCAG 2 cut(s) 9, 366
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 2 cut(s) 149, 335
BseSI GKGCMC 1 cut(s) 365
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 2 cut(s) 387, 509
BseYI CCCAGC 2 cut(s) 583, 697
Bsh1236I CGCG 2 cut(s) 521, 590
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 131, 395, 566, 649
BshNI GGYRCC 1 cut(s) 532
BsiEI CGRYCG 1 cut(s) 132
BsiSI CCGG 2 cut(s) 242, 258
BslFI GGGAC 3 cut(s) 407, 658, 670
BslI CCNNNNNNNGG 5 cut(s) 257, 391, 430, 656, 681
BsmAI GTCTC 1 cut(s) 506
BsmFI GGGAC 3 cut(s) 407, 658, 670
BsnI GGCC 4 cut(s) 131, 395, 566, 649
Bso31I GGTCTC 1 cut(s) 506
Bsp119I TTCGAA 1 cut(s) 482
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 4 cut(s) 52, 222, 417, 766
BspANI GGCC 4 cut(s) 131, 395, 566, 649
BspCNI CTCAG 2 cut(s) 10, 367
BspFNI CGCG 2 cut(s) 521, 590
BspLI GGNNCC 4 cut(s) 229, 434, 534, 686
BspPI GGATC 1 cut(s) 774
BspT104I TTCGAA 1 cut(s) 482
BspT107I GGYRCC 1 cut(s) 532
BspTNI GGTCTC 1 cut(s) 506
BsrBI CCGCTC 1 cut(s) 128
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 4 cut(s) 390, 469, 519, 561
BssMI GATC 4 cut(s) 52, 222, 417, 766
BssNAI GTATAC 1 cut(s) 798
BssNI GRCGYC 1 cut(s) 533
Bst1107I GTATAC 1 cut(s) 798
Bst4CI ACNGT 1 cut(s) 773
Bst6I CTCTTC 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 533
BstBI TTCGAA 1 cut(s) 482
BstC8I GCNNGC 3 cut(s) 476, 568, 607
BstDEI CTNAG 2 cut(s) 18, 375
BstDSI CCRYGG 2 cut(s) 390, 519
BstF5I GGATG 4 cut(s) 182, 583, 690, 775
BstFNI CGCG 2 cut(s) 521, 590
BstH2I RGCGCY 4 cut(s) 375, 536, 596, 635
BstHHI GCGC 5 cut(s) 374, 535, 590, 595, 634
BstKTI GATC 4 cut(s) 55, 225, 420, 769
BstMAI GTCTC 1 cut(s) 506
BstMBI GATC 4 cut(s) 52, 222, 417, 766
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 369, 599
BstSCI CCNGG 1 cut(s) 240
BstSLI GKGCMC 1 cut(s) 365
BstUI CGCG 2 cut(s) 521, 590
BstV1I GCAGC 2 cut(s) 387, 509
BstX2I RGATCY 1 cut(s) 52
BstXI CCANNNNNNTGG 1 cut(s) 574
BstYI RGATCY 1 cut(s) 52
BstZ17I GTATAC 1 cut(s) 798
BstZI CGGCCG 1 cut(s) 129
BsuI GTATCC 1 cut(s) 217
BsuRI GGCC 4 cut(s) 131, 395, 566, 649
BtgI CCRYGG 2 cut(s) 390, 519
BtrI CACGTC 1 cut(s) 645
BtsCI GGATG 4 cut(s) 182, 583, 690, 775
BtsIMutI CAGTG 1 cut(s) 365
Cac8I GCNNGC 3 cut(s) 476, 568, 607
CciNI GCGGCCGC 1 cut(s) 129
CfoI GCGC 5 cut(s) 374, 535, 590, 595, 634
Cfr13I GGNCC 3 cut(s) 433, 451, 684
Cfr42I CCGCGG 1 cut(s) 522
CseI GACGC 1 cut(s) 559
Csp6I GTAC 1 cut(s) 11
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 2 cut(s) 18, 375
DinI GGCGCC 1 cut(s) 534
DpnI GATC 4 cut(s) 54, 224, 419, 768
DpnII GATC 4 cut(s) 52, 222, 417, 766
DraIII CACNNNGTG 1 cut(s) 391
EaeI YGGCCR 4 cut(s) 129, 393, 564, 647
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
EciI GGCGGA 2 cut(s) 15, 137
EclXI CGGCCG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 506
Eco47I GGWCC 3 cut(s) 433, 451, 684
Eco52I CGGCCG 1 cut(s) 129
EcoO109I RGGNCCY 2 cut(s) 433, 684
EgeI GGCGCC 1 cut(s) 534
EheI GGCGCC 1 cut(s) 534
FaiI YATR 3 cut(s) 744, 794, 798
FaqI GGGAC 3 cut(s) 407, 658, 670
FblI GTMKAC 1 cut(s) 797
FokI GGATG 4 cut(s) 169, 590, 677, 762
GlaI GCGC 5 cut(s) 373, 534, 589, 594, 633
GsaI CCCAGC 2 cut(s) 587, 701
GsuI CTGGAG 1 cut(s) 530
HaeII RGCGCY 4 cut(s) 375, 536, 596, 635
HaeIII GGCC 4 cut(s) 131, 395, 566, 649
HapII CCGG 2 cut(s) 242, 258
HgaI GACGC 1 cut(s) 559
HhaI GCGC 5 cut(s) 374, 535, 590, 595, 634
Hin1I GRCGYC 1 cut(s) 533
Hin6I GCGC 5 cut(s) 372, 533, 588, 593, 632
HinP1I GCGC 5 cut(s) 372, 533, 588, 593, 632
HinfI GANTC 7 cut(s) 22, 332, 439, 446, 713, 725, 785
HpaII CCGG 2 cut(s) 242, 258
HphI GGTGA 2 cut(s) 69, 404
Hpy166II GTNNAC 1 cut(s) 798
Hpy188I TCNGA 4 cut(s) 227, 470, 659, 790
Hpy188III TCNNGA 5 cut(s) 47, 436, 443, 454, 616
Hpy8I GTNNAC 1 cut(s) 798
Hpy99I CGWCG 1 cut(s) 575
HpyAV CCTTC 5 cut(s) 95, 106, 262, 364, 478
HpyCH4III ACNGT 1 cut(s) 773
HpyCH4IV ACGT 2 cut(s) 204, 644
HpyCH4V TGCA 1 cut(s) 297
HpyF10VI GCNNNNNNNGC 2 cut(s) 369, 599
HpyF3I CTNAG 2 cut(s) 18, 375
HpySE526I ACGT 2 cut(s) 204, 644
Hsp92I GRCGYC 1 cut(s) 533
HspAI GCGC 5 cut(s) 372, 533, 588, 593, 632
KasI GGCGCC 1 cut(s) 532
KspI CCGCGG 1 cut(s) 522
Kzo9I GATC 4 cut(s) 52, 222, 417, 766
LmnI GCTCC 2 cut(s) 125, 659
Lsp1109I GCAGC 2 cut(s) 387, 509
LweI GCATC 2 cut(s) 129, 371
MaeII ACGT 2 cut(s) 204, 644
MaeIII GTNAC 2 cut(s) 75, 640
MalI GATC 4 cut(s) 54, 224, 419, 768
MbiI CCGCTC 1 cut(s) 128
MboI GATC 4 cut(s) 52, 222, 417, 766
MboII GAAGA 3 cut(s) 17, 62, 187
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 365
MlsI TGGCCA 1 cut(s) 395
MluNI TGGCCA 1 cut(s) 395
Mly113I GGCGCC 1 cut(s) 533
MlyI GAGTC 1 cut(s) 440
Mox20I TGGCCA 1 cut(s) 395
MscI TGGCCA 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 2 cut(s) 242, 258
MspR9I CCNGG 1 cut(s) 242
MteI GCGCNGCGC 1 cut(s) 591
MvnI CGCG 2 cut(s) 521, 590
MwoI GCNNNNNNNGC 2 cut(s) 369, 599
NarI GGCGCC 1 cut(s) 533
NciI CCSGG 1 cut(s) 242
NdeII GATC 4 cut(s) 52, 222, 417, 766
NlaIV GGNNCC 4 cut(s) 229, 434, 534, 686
NmeAIII GCCGAG 1 cut(s) 542
NmuCI GTSAC 2 cut(s) 75, 640
NotI GCGGCCGC 1 cut(s) 129
NspV TTCGAA 1 cut(s) 482
PfeI GAWTC 6 cut(s) 22, 332, 439, 713, 725, 785
PflFI GACNNNGTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 240
PleI GAGTC 1 cut(s) 440
PluTI GGCGCC 1 cut(s) 536
PpsI GAGTC 1 cut(s) 440
PpuMI RGGWCCY 2 cut(s) 433, 684
Psp5II RGGWCCY 2 cut(s) 433, 684
PspFI CCCAGC 2 cut(s) 583, 697
PspN4I GGNNCC 4 cut(s) 229, 434, 534, 686
PspPI GGNCC 3 cut(s) 433, 451, 684
PspPPI RGGWCCY 2 cut(s) 433, 684
PsuI RGATCY 1 cut(s) 52
PsyI GACNNNGTC 1 cut(s) 449
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
SacII CCGCGG 1 cut(s) 522
Sau3AI GATC 4 cut(s) 52, 222, 417, 766
Sau96I GGNCC 3 cut(s) 433, 451, 684
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 1 cut(s) 365
SfaNI GCATC 2 cut(s) 129, 371
SfoI GGCGCC 1 cut(s) 534
Sfr303I CCGCGG 1 cut(s) 522
SfuI TTCGAA 1 cut(s) 482
SgrBI CCGCGG 1 cut(s) 522
SinI GGWCC 3 cut(s) 433, 451, 684
SspDI GGCGCC 1 cut(s) 532
SspI AATATT 1 cut(s) 554
StyD4I CCNGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 773
TaiI ACGT 2 cut(s) 207, 647
TaqI TCGA 5 cut(s) 35, 270, 444, 482, 716
TaqII GACCGA 1 cut(s) 439
TatI WGTACW 1 cut(s) 10
TauI GCSGC 8 cut(s) 31, 131, 134, 497, 593, 605, 632, 652
TfiI GAWTC 6 cut(s) 22, 332, 439, 713, 725, 785
TscAI CASTG 1 cut(s) 365
TseFI GTSAC 2 cut(s) 75, 640
TseI GCWGC 2 cut(s) 400, 497
Tsp45I GTSAC 2 cut(s) 75, 640
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 1 cut(s) 365
Tth111I GACNNNGTC 1 cut(s) 449
Van91I CCANNNNNTGG 1 cut(s) 391
VpaK11BI GGWCC 3 cut(s) 433, 451, 684
XmiI GTMKAC 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.