AT3G26410

tRNA (guanine(10)-N2)-methyltransferase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014379
Physical Location & Seq
Reverse (-)
9669415 .. 9671348
1934 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G26410.1

Sequence Viewer

Length: 1434 bp
ATGTGGTTTCTGTGTGTCTTCTATCACAGGCTATTAGACTTCAGAAAGCCAGAAGTGGAAGCTTTAGCTGAACTCTTCGGAGAAGAGATTGCAGAGAATGAGTCTCTCCAATGGCGTCTACCTGAAAATCACCATAACGATACTCCATTCCATTTCGTTCAACTTTCATCTGAAGAAATCGCTCGTAACATTGCTAAACGAAGCATATTGGTGAAAGGAATGTATGAGCTTTGGGGTGAAGGAACTTGTTATGAAGAGCTTAAAGACTCGATTCAAAGCTACCCTGATTCTCGAAAGCTTCCATTTCTCACTTCTGATTCAACTTTTAGAATCTCTGTTGAAACTTTTGGAAAGGCTTTAACTTTTGACGAGCAGAAAGATCGAATCCAGTCGTTTACTTATATACCATTTGAGGGTCGTGTTAACTTGAAGAACCCAGATCATAACTTTTTTCTAATGGAAATGATTGAATCTGAAGAGAACAATGGACTTCAACCTATTCTACAGAGAAGGATATTTTTTGGGAGGGAGGTTGGTTTTGCTGATAGGAAGCTTTTACCTACATTTCAGTTGAAATCTCGTACTTACCTTGGTCCAACTGCTATGGATGCTGAAATGGCTTTCTTAATGGCTAATCAAGCGAAAGCTACTTCGGGAAAACTTGTGTATGATCCTTTTGTTGGTACTGGGAGCATTCTTGTTTCTGCTGCACGCTTTGGCGCAATGACAATGGGTGCAGATATCGATATCAGAGTAGTGCGTGATGGACGTGGTCCAGATTGTAATGTTTGGAGCAATTTCAAACAGTATGGATTACCAATGCCGGTAGCTTTACTTAGAATGGATAATAACGTTCCTCCTTGGCGATCGGGGCTAAAGGAGATCTTTGATGCAATTATCTGCGATCCACCTTACGGGGTTCGTGCTGGTGGACGTAAATCCGGTGGTAGGAAAATCCTTAGAGGAACAGTGGATCCTTACACTGTCCCTGATGACAAAAGAACAGATCACATTCCATCCACTGGTGCATATAGTCTAGTGGAGTGTGTTCATGATCTACTTCATCTTGCTGCAAGAATGTTGGTGATGAAAGGCAGGCTCGTCTTTTTCTTCCCGGTTTTGAGAGATGAGACTGGCAGCGAGGTTAAGTTCCCGGAGCACCCGTGTTTCAAGTTAGTAGCTGTATCTGAACAGATCCTGAGCTCGCGGTACAGTCGGGTTCTGCTAACGATGGTGAAAGTAGAGCCTTATAGTGAAGAGGTTGAAGAAGCTGCTCGTTTAATGCATTTAGAGTTTAGAGAGAATCATCTTAAGTGGTTAGAGGATGGTAATATTCACTCCTCTATATTCAAACCCATTGATTCTTCTCAGATTGACACTGATTCCAAAGCCTTCAAAGATCCAAAACCTAAGTATAGAGGGAAGTATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0020

Protein Analysis

477

Amino Acids

54.64

Weight (kDa)

6.22

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmrp11_N PF25904 3 - 183 4e-33 Tmrp11 THUMP N-terminal domain
UPF0020 PF01170 193 - 314 3.2e-11 RMKL-like, methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26410
fragaria_vesca FvH4_6g05370
malus_domestica MD12G1223100.v1.1
prunus_persica Prupe.6G329700_v2.0.a1
pyrus_communis pycom12g20900
rosa_chinensis RchiOBHm_Chr3g0454151
rosa_laevigata RLG00000025478
rosa_multiflora Rmu_sc0004479.1_g000008 Rmu_sc0018584.1_g000001
rosa_roxburghii Rroxscaffold_165G00436930 Rroxscaffold_6G00425430
rosa_rugosa Rorug02G0653500
rosa_samantha Rh3AG056400 Rh3BG057800 Rh3CG057100 Rh3DG058300
rosa_wichuraiana Rw3G004330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1022
AccI GTMKAC 1 cut(s) 118
AccII CGCG 1 cut(s) 1207
AciI CCGC 1 cut(s) 1207
AclI AACGTT 1 cut(s) 852
AclWI GGATC 6 cut(s) 665, 899, 968, 981, 1189, 1394
AcuI CTGAAG 3 cut(s) 25, 192, 495
AcyI GRCGYC 1 cut(s) 115
AfaI GTAC 3 cut(s) 583, 685, 1211
AfiI CCNNNNNNNGG 5 cut(s) 413, 680, 914, 948, 1022
AflII CTTAAG 1 cut(s) 1310
AjiI CACGTC 1 cut(s) 770
AloI GAACNNNNNNTCC 2 cut(s) 958, 990
Alw21I GWGCWC 2 cut(s) 1161, 1205
Alw26I GTCTC 2 cut(s) 108, 1124
AlwI GGATC 6 cut(s) 665, 899, 968, 981, 1189, 1394
AlwNI CAGNNNCTG 1 cut(s) 1198
ApeKI GCWGC 4 cut(s) 707, 1070, 1137, 1271
AspLEI GCGC 1 cut(s) 722
AspS9I GGNCC 2 cut(s) 593, 773
AsuC2I CCSGG 2 cut(s) 1115, 1154
AsuHPI GGTGA 5 cut(s) 122, 223, 248, 1096, 1246
AvaII GGWCC 2 cut(s) 593, 773
BamHI GGATCC 1 cut(s) 973
BanII GRGCYC 1 cut(s) 1205
BbsI GAAGAC 1 cut(s) 10
Bbv12I GWGCWC 2 cut(s) 1161, 1205
BbvI GCAGC 4 cut(s) 694, 1057, 1149, 1258
BccI CCATC 4 cut(s) 758, 1024, 1225, 1319
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BcnI CCSGG 2 cut(s) 1115, 1154
BcoDI GTCTC 2 cut(s) 108, 1124
BfaI CTAG 1 cut(s) 1037
BfmI CTRYAG 1 cut(s) 503
BfrI CTTAAG 1 cut(s) 1310
BglII AGATCT 1 cut(s) 882
BisI GCNGC 4 cut(s) 708, 1071, 1138, 1272
BlsI GCNGC 4 cut(s) 709, 1072, 1139, 1273
Bme1390I CCNGG 2 cut(s) 1115, 1154
Bme18I GGWCC 2 cut(s) 593, 773
BmgBI CACGTC 1 cut(s) 770
BmgT120I GGNCC 2 cut(s) 593, 773
BmiI GGNNCC 1 cut(s) 975
BmrFI CCNGG 2 cut(s) 1115, 1154
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 2 cut(s) 598, 880
BmuI ACTGGG 1 cut(s) 696
BpiI GAAGAC 1 cut(s) 10
Bpu10I CCTNAGC 1 cut(s) 1199
BpuMI CCSGG 2 cut(s) 1115, 1154
Bsa29I ATCGAT 1 cut(s) 744
BsaHI GRCGYC 1 cut(s) 115
BsaJI CCNNGG 2 cut(s) 589, 860
BsaWI WCCGGW 1 cut(s) 941
BsaXI ACNNNNNCTCC 2 cut(s) 1148, 1178
Bsc4I CCNNNNNNNGG 5 cut(s) 413, 680, 914, 948, 1022
Bse118I RCCGGY 1 cut(s) 823
Bse1I ACTGG 4 cut(s) 388, 691, 1027, 1138
Bse3DI GCAATG 2 cut(s) 189, 729
BseCI ATCGAT 1 cut(s) 744
BseDI CCNNGG 2 cut(s) 589, 860
BseGI GGATG 3 cut(s) 613, 1016, 1330
BseLI CCNNNNNNNGG 5 cut(s) 413, 680, 914, 948, 1022
BseMI GCAATG 2 cut(s) 189, 729
BseMII CTCAG 2 cut(s) 1190, 1382
BseNI ACTGG 4 cut(s) 388, 691, 1027, 1138
BseRI GAGGAG 1 cut(s) 1330
BseXI GCAGC 4 cut(s) 694, 1057, 1149, 1258
BsgI GTGCAG 2 cut(s) 693, 756
Bsh1236I CGCG 1 cut(s) 1207
Bsh1285I CGRYCG 1 cut(s) 869
BshVI ATCGAT 1 cut(s) 744
BsiEI CGRYCG 1 cut(s) 869
BsiHKAI GWGCWC 2 cut(s) 1161, 1205
BsiSI CCGG 4 cut(s) 824, 942, 1115, 1154
BslFI GGGAC 1 cut(s) 971
BslI CCNNNNNNNGG 5 cut(s) 413, 680, 914, 948, 1022
BsmAI GTCTC 2 cut(s) 108, 1124
BsmFI GGGAC 1 cut(s) 971
BsmI GAATGC 1 cut(s) 693
Bsp1286I GDGCHC 2 cut(s) 1161, 1205
BspACI CCGC 1 cut(s) 1207
BspCNI CTCAG 2 cut(s) 1191, 1381
BspDI ATCGAT 1 cut(s) 744
BspFNI CGCG 1 cut(s) 1207
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 1 cut(s) 975
BspPI GGATC 6 cut(s) 665, 899, 968, 981, 1189, 1394
BspQI GCTCTTC 1 cut(s) 249
BspTI CTTAAG 1 cut(s) 1310
BsrDI GCAATG 2 cut(s) 189, 729
BsrFI RCCGGY 1 cut(s) 823
BsrI ACTGG 4 cut(s) 388, 691, 1027, 1138
BssAI RCCGGY 1 cut(s) 823
BssECI CCNNGG 2 cut(s) 589, 860
BssNI GRCGYC 1 cut(s) 115
BssT1I CCWWGG 2 cut(s) 589, 860
Bst4CI ACNGT 4 cut(s) 807, 970, 985, 1214
Bst6I CTCTTC 5 cut(s) 78, 80, 249, 471, 1251
BstACI GRCGYC 1 cut(s) 115
BstAFI CTTAAG 1 cut(s) 1310
BstC8I GCNNGC 3 cut(s) 712, 1097, 1205
BstDEI CTNAG 5 cut(s) 836, 959, 1199, 1368, 1410
BstF5I GGATG 3 cut(s) 613, 1016, 1330
BstFNI CGCG 1 cut(s) 1207
BstHHI GCGC 1 cut(s) 722
BstMAI GTCTC 2 cut(s) 108, 1124
BstMCI CGRYCG 1 cut(s) 869
BstMWI GCNNNNNNNGC 4 cut(s) 608, 617, 638, 871
BstSCI CCNGG 2 cut(s) 1113, 1152
BstSFI CTRYAG 1 cut(s) 503
BstUI CGCG 1 cut(s) 1207
BstV1I GCAGC 4 cut(s) 694, 1057, 1149, 1258
BstV2I GAAGAC 1 cut(s) 10
BstX2I RGATCY 4 cut(s) 882, 973, 1194, 1399
BstYI RGATCY 4 cut(s) 882, 973, 1194, 1399
Bsu15I ATCGAT 1 cut(s) 744
BsuTUI ATCGAT 1 cut(s) 744
BtrI CACGTC 1 cut(s) 770
BtsCI GGATG 3 cut(s) 613, 1016, 1330
BtsIMutI CAGTG 4 cut(s) 975, 981, 1020, 1377
Cac8I GCNNGC 3 cut(s) 712, 1097, 1205
CaiI CAGNNNCTG 1 cut(s) 1198
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 1 cut(s) 722
Cfr10I RCCGGY 1 cut(s) 823
Cfr13I GGNCC 2 cut(s) 593, 773
ClaI ATCGAT 1 cut(s) 744
CseI GACGC 1 cut(s) 104
Csp6I GTAC 3 cut(s) 582, 684, 1210
CviAII CATG 1 cut(s) 1052
CviQI GTAC 3 cut(s) 582, 684, 1210
DdeI CTNAG 5 cut(s) 836, 959, 1199, 1368, 1410
Eam1104I CTCTTC 5 cut(s) 78, 80, 249, 471, 1251
EarI CTCTTC 5 cut(s) 78, 80, 249, 471, 1251
Ecl136II GAGCTC 1 cut(s) 1203
Eco130I CCWWGG 2 cut(s) 589, 860
Eco24I GRGCYC 1 cut(s) 1205
Eco32I GATATC 2 cut(s) 742, 748
Eco47I GGWCC 2 cut(s) 593, 773
Eco53kI GAGCTC 1 cut(s) 1203
Eco57I CTGAAG 3 cut(s) 25, 192, 495
EcoICRI GAGCTC 1 cut(s) 1203
EcoRV GATATC 2 cut(s) 742, 748
EcoT14I CCWWGG 2 cut(s) 589, 860
EcoT22I ATGCAT 1 cut(s) 1287
EcoT38I GRGCYC 1 cut(s) 1205
ErhI CCWWGG 2 cut(s) 589, 860
FaeI CATG 1 cut(s) 1055
FalI AAGNNNNNCTT 2 cut(s) 869, 901
FaqI GGGAC 1 cut(s) 971
FatI CATG 1 cut(s) 1051
FblI GTMKAC 1 cut(s) 118
Fnu4HI GCNGC 4 cut(s) 708, 1071, 1138, 1272
FokI GGATG 3 cut(s) 620, 1003, 1337
FriOI GRGCYC 1 cut(s) 1205
Fsp4HI GCNGC 4 cut(s) 708, 1071, 1138, 1272
FspBI CTAG 1 cut(s) 1037
GlaI GCGC 1 cut(s) 721
GluI GCNGC 4 cut(s) 708, 1071, 1138, 1272
HapII CCGG 4 cut(s) 824, 942, 1115, 1154
HgaI GACGC 1 cut(s) 104
HhaI GCGC 1 cut(s) 722
Hin1I GRCGYC 1 cut(s) 115
Hin1II CATG 1 cut(s) 1055
Hin6I GCGC 1 cut(s) 720
HinP1I GCGC 1 cut(s) 720
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 3 cut(s) 60, 296, 551
HpaI GTTAAC 1 cut(s) 424
HpaII CCGG 4 cut(s) 824, 942, 1115, 1154
HphI GGTGA 5 cut(s) 122, 223, 248, 1096, 1246
Hpy166II GTNNAC 4 cut(s) 119, 396, 424, 932
Hpy188I TCNGA 8 cut(s) 44, 80, 172, 316, 475, 752, 1189, 1371
Hpy188III TCNNGA 5 cut(s) 291, 654, 776, 1052, 1198
Hpy8I GTNNAC 4 cut(s) 119, 396, 424, 932
HpyAV CCTTC 3 cut(s) 233, 504, 1402
HpyCH4III ACNGT 4 cut(s) 807, 970, 985, 1214
HpyCH4IV ACGT 3 cut(s) 769, 852, 934
HpyCH4V TGCA 7 cut(s) 92, 710, 737, 893, 1028, 1073, 1285
HpyF10VI GCNNNNNNNGC 4 cut(s) 608, 617, 638, 871
HpyF3I CTNAG 5 cut(s) 836, 959, 1199, 1368, 1410
HpySE526I ACGT 3 cut(s) 769, 852, 934
Hsp92I GRCGYC 1 cut(s) 115
Hsp92II CATG 1 cut(s) 1055
HspAI GCGC 1 cut(s) 720
KspAI GTTAAC 1 cut(s) 424
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 3 cut(s) 690, 792, 1156
Lsp1109I GCAGC 4 cut(s) 694, 1057, 1149, 1258
LweI GCATC 2 cut(s) 598, 880
MaeI CTAG 1 cut(s) 1037
MaeII ACGT 3 cut(s) 769, 852, 934
MaeIII GTNAC 1 cut(s) 185
MflI RGATCY 4 cut(s) 882, 973, 1194, 1399
MhlI GDGCHC 2 cut(s) 1161, 1205
MluCI AATT 2 cut(s) 796, 894
MlyI GAGTC 2 cut(s) 110, 260
MmeI TCCRAC 1 cut(s) 620
Mph1103I ATGCAT 1 cut(s) 1287
MseI TTAA 7 cut(s) 261, 359, 423, 626, 1146, 1280, 1311
MslI CAYNNNNRTG 1 cut(s) 209
MspCI CTTAAG 1 cut(s) 1310
MspI CCGG 4 cut(s) 824, 942, 1115, 1154
MspR9I CCNGG 2 cut(s) 1115, 1154
Mva1269I GAATGC 1 cut(s) 693
MvnI CGCG 1 cut(s) 1207
MwoI GCNNNNNNNGC 4 cut(s) 608, 617, 638, 871
NciI CCSGG 2 cut(s) 1115, 1154
NlaIII CATG 1 cut(s) 1055
NlaIV GGNNCC 1 cut(s) 975
NsiI ATGCAT 1 cut(s) 1287
PagI TCATGA 1 cut(s) 1051
PciSI GCTCTTC 1 cut(s) 249
PctI GAATGC 1 cut(s) 693
PfeI GAWTC 9 cut(s) 271, 287, 317, 330, 384, 470, 1303, 1361, 1382
PflFI GACNNNGTC 1 cut(s) 771
PflMI CCANNNNNTGG 1 cut(s) 1022
PfoI TCCNGGA 1 cut(s) 1152
PkrI GCNGC 4 cut(s) 709, 1072, 1139, 1273
Ple19I CGATCG 1 cut(s) 869
PleI GAGTC 2 cut(s) 109, 260
PpsI GAGTC 2 cut(s) 109, 260
Psp124BI GAGCTC 1 cut(s) 1205
Psp1406I AACGTT 1 cut(s) 852
PspN4I GGNNCC 1 cut(s) 975
PspPI GGNCC 2 cut(s) 593, 773
PstNI CAGNNNCTG 1 cut(s) 1198
PsuI RGATCY 4 cut(s) 882, 973, 1194, 1399
PsyI GACNNNGTC 1 cut(s) 771
PvuI CGATCG 1 cut(s) 869
RsaI GTAC 3 cut(s) 583, 685, 1211
RsaNI GTAC 3 cut(s) 582, 684, 1210
RseI CAYNNNNRTG 1 cut(s) 209
SacI GAGCTC 1 cut(s) 1205
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 7 cut(s) 261, 359, 423, 626, 1146, 1280, 1311
SatI GCNGC 4 cut(s) 708, 1071, 1138, 1272
Sau96I GGNCC 2 cut(s) 593, 773
SchI GAGTC 2 cut(s) 110, 260
ScrFI CCNGG 2 cut(s) 1115, 1154
SduI GDGCHC 2 cut(s) 1161, 1205
SfaNI GCATC 2 cut(s) 598, 880
SfcI CTRYAG 1 cut(s) 503
SinI GGWCC 2 cut(s) 593, 773
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 1310
SmoI CTYRAG 1 cut(s) 1310
Sse9I AATT 2 cut(s) 796, 894
SsiI CCGC 1 cut(s) 1207
SspI AATATT 1 cut(s) 1333
SspMI CTAG 1 cut(s) 1037
SstI GAGCTC 1 cut(s) 1205
StyD4I CCNGG 2 cut(s) 1113, 1152
StyI CCWWGG 2 cut(s) 589, 860
TaaI ACNGT 4 cut(s) 807, 970, 985, 1214
TaiI ACGT 3 cut(s) 772, 855, 937
TaqI TCGA 4 cut(s) 269, 292, 382, 744
TasI AATT 2 cut(s) 796, 894
TfiI GAWTC 9 cut(s) 271, 287, 317, 330, 384, 470, 1303, 1361, 1382
Tru1I TTAA 7 cut(s) 261, 359, 423, 626, 1146, 1280, 1311
Tru9I TTAA 7 cut(s) 261, 359, 423, 626, 1146, 1280, 1311
TscAI CASTG 4 cut(s) 975, 988, 1027, 1384
TseI GCWGC 4 cut(s) 707, 1070, 1137, 1271
TspDTI ATGAA 5 cut(s) 156, 267, 1040, 1052, 1103
TspRI CASTG 4 cut(s) 975, 988, 1027, 1384
Tth111I GACNNNGTC 1 cut(s) 771
Van91I CCANNNNNTGG 1 cut(s) 1022
Vha464I CTTAAG 1 cut(s) 1310
VpaK11BI GGWCC 2 cut(s) 593, 773
XmiI GTMKAC 1 cut(s) 118
XspI CTAG 1 cut(s) 1037
Zsp2I ATGCAT 1 cut(s) 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.