MD12G1223100.v1.1

tRNA (Guanine(10)-N2)-methyltransferase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014379
Physical Location & Seq
Reverse (-)
29994152 .. 29997704
3553 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1223100.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGTGGTATCTGTGCATGTTCTACCACCGACTGTTAGACTACCGGAAAGCCGAGGTGGAATCTTTAGCCCAACTATTCGCCAACGACGAAGAAGATCAGTACAAAACGCAGCGTTTGAAATGGCGGCTGCCGCTCCACCACCACCCGGATTCCCCTTTCCATTTCGTCGACCTCCCTTCCGAAGACGTCGCCAGGAACGTCGCCAATCGAAGTATACTTGTGAAGGGAATGTATGAGCTTTGGGGAGAAGGGGGTAGCTATGAGGAGCTGGAAGAGGCTATTAGAAGTTATCCGGATGAGCGGAAGTTGCCGTACCTAGAGCCTGATAGTACTTTCAAGATTAATGTTGACACATTTGGTAAGGTCATCAGCCTCCAGGAGCAAACACAACGGATTCATGGACTTAGCTACATCCCTTTCAAGGGTCGAGTTAATTTGAAAAGTCCAGAGCACAAGTTCTGGCTTATGGAAACTGATGATTATGGAGTTAACAATGGTCTTCCGCCAATTGTCCAAAGAAGAATCTTTTTCGGTCGCGAAATTGGTGGTGCTGATCGAAAGCTTCTACCAACATACCAGTTGAAAAGCCGTACTTATCTTGGCCCAACAGCCATGGATGCAGAAATTGCTTTCTTGATGGCCAACCAAGCATTGGTCACGCCAGGGAAGCTTGTATACGACCCTTTTGTTGGCACCGGGAGCATTCTTATAGCTGCTGCTCATTTTGGAGCCATGACAATGGGTGCAGATATTGATATCAGGGTGGTACGAGATGGCCGTGGCCCTGATTGTAATGTCTGGAGTAATTTCAAGCAGTACGGATTACCAATGCCAATTGCTTTGTTAAGGGCAGATAACAATCTACCTCCTTGGCGTACTGGATTGAAAGAGGTCTTTGATGCCATAATTTGTGACCCTCCTTATGGTGTTCGTGCTGGGGGACGCAAATCTGGTGGCCGGAAGTTGATGAAAGGGGTTGTCGGCCCTTACACCGTTCCTGATGACAAGAGGACAGACCACATACCATCAACTGCATCTTACAGCTTGGTCGAGTGTGTGCACGACTTGCTTGACCTAGCCGGCAGGATGCTTGTAATGGGTGGGAGGCTTGTGTTCTTCTACCCTGTAGTGAGAGAAGATGATAATGTCAATAACTCTTTCCCAGAGCACCCGTGTTTCCAACTGATTGCTACTTCAGAGCAGATACTGAGCTCACGTTACAGTAGAGTACTACTGACCATGGTTAAGACAAGTCAATACACCGAAGAAATTGCAGAAGAAGCCAGATTAAAACACAAAGACTTCAAGGAGAACCATCTGAAGTGGTTGGAAGATGGAAACCTTCATTCCGCAGTTTTCAGTCCTTCTGATCTTCAAGTGAATGGTGCTGCTGATGCCAAAACCAGTAAAGAACCAAAGCCAAAATACAGAGGCAAGTATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0020

Protein Analysis

482

Amino Acids

54.7

Weight (kDa)

6.72

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmrp11_N PF25904 2 - 186 3.9e-29 Tmrp11 THUMP N-terminal domain
UPF0020 PF01170 196 - 320 6.8e-13 RMKL-like, methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26410
fragaria_vesca FvH4_6g05370
malus_domestica MD12G1223100.v1.1
prunus_persica Prupe.6G329700_v2.0.a1
pyrus_communis pycom12g20900
rosa_chinensis RchiOBHm_Chr3g0454151
rosa_laevigata RLG00000025478
rosa_multiflora Rmu_sc0004479.1_g000008 Rmu_sc0018584.1_g000001
rosa_roxburghii Rroxscaffold_165G00436930 Rroxscaffold_6G00425430
rosa_rugosa Rorug02G0653500
rosa_samantha Rh3AG056400 Rh3BG057800 Rh3CG057100 Rh3DG058300
rosa_wichuraiana Rw3G004330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AccB1I GGYRCC 1 cut(s) 692
AccB7I CCANNNNNTGG 1 cut(s) 652
AccBSI CCGCTC 2 cut(s) 133, 301
AccI GTMKAC 3 cut(s) 168, 214, 675
AccII CGCG 1 cut(s) 537
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 5 cut(s) 124, 131, 301, 503, 1350
AcoI YGGCCR 3 cut(s) 639, 775, 955
AcuI CTGAAG 2 cut(s) 1179, 1340
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 8 cut(s) 101, 314, 331, 592, 768, 818, 877, 1230
AfiI CCNNNNNNNGG 6 cut(s) 145, 421, 422, 652, 689, 923
AgsI TTSAA 9 cut(s) 118, 337, 421, 439, 583, 811, 886, 1306, 1376
AjnI CCWGG 3 cut(s) 191, 375, 661
AluBI AGCT 9 cut(s) 238, 258, 268, 408, 562, 670, 713, 1044, 1212
AluI AGCT 9 cut(s) 238, 258, 268, 408, 562, 670, 713, 1044, 1212
Alw21I GWGCWC 4 cut(s) 453, 1062, 1170, 1214
Alw44I GTGCAC 1 cut(s) 1058
AlwNI CAGNNNCTG 1 cut(s) 1207
Aor13HI TCCGGA 1 cut(s) 292
AoxI GGCC 6 cut(s) 601, 639, 775, 781, 955, 982
ApaLI GTGCAC 1 cut(s) 1058
ApeKI GCWGC 5 cut(s) 109, 127, 713, 716, 1388
AseI ATTAAT 1 cut(s) 342
AspS9I GGNCC 3 cut(s) 602, 782, 983
AsuC2I CCSGG 2 cut(s) 146, 697
BaeGI GKGCMC 1 cut(s) 1062
BalI TGGCCA 1 cut(s) 641
BanI GGYRCC 1 cut(s) 692
BanII GRGCYC 1 cut(s) 1214
BarI GAAGNNNNNNTAC 4 cut(s) 296, 328, 659, 691
BbsI GAAGAC 2 cut(s) 189, 491
Bbv12I GWGCWC 4 cut(s) 453, 1062, 1170, 1214
BbvI GCAGC 5 cut(s) 114, 121, 700, 703, 1375
BccI CCATC 5 cut(s) 631, 767, 1033, 1323, 1328
BceAI ACGGC 3 cut(s) 295, 573, 762
BcgI CGANNNNNNTGC 2 cut(s) 1253, 1287
BciT130I CCWGG 3 cut(s) 193, 377, 663
BcnI CCSGG 2 cut(s) 146, 697
BfaI CTAG 2 cut(s) 317, 1076
BfmI CTRYAG 1 cut(s) 1125
BisI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
BlsI GCNGC 7 cut(s) 111, 126, 129, 132, 715, 718, 1390
BmcAI AGTACT 2 cut(s) 331, 1230
Bme1390I CCNGG 5 cut(s) 146, 193, 377, 663, 697
BmgT120I GGNCC 3 cut(s) 602, 782, 983
BmiI GGNNCC 2 cut(s) 694, 730
BmrFI CCNGG 5 cut(s) 146, 193, 377, 663, 697
BmsI GCATC 5 cut(s) 607, 889, 1043, 1077, 1384
BpiI GAAGAC 2 cut(s) 189, 491
BpmI CTGGAG 2 cut(s) 359, 820
BpuMI CCSGG 2 cut(s) 146, 697
BsaBI GATNNNNATC 1 cut(s) 858
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 6 cut(s) 51, 612, 662, 778, 869, 1239
BsaWI WCCGGW 2 cut(s) 42, 292
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 421, 422, 652, 689, 923
Bse118I RCCGGY 1 cut(s) 1079
Bse1I ACTGG 3 cut(s) 577, 883, 1404
Bse8I GATNNNNATC 1 cut(s) 858
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 3 cut(s) 193, 377, 663
BseDI CCNNGG 6 cut(s) 51, 612, 662, 778, 869, 1239
BseGI GGATG 4 cut(s) 301, 411, 622, 1092
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 6 cut(s) 145, 421, 422, 652, 689, 923
BseMII CTCAG 1 cut(s) 1199
BseNI ACTGG 3 cut(s) 577, 883, 1404
BseRI GAGGAG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 1062
BseXI GCAGC 5 cut(s) 114, 121, 700, 703, 1375
BseYI CCCAGC 1 cut(s) 935
BsgI GTGCAG 1 cut(s) 765
Bsh1236I CGCG 1 cut(s) 537
Bsh1285I CGRYCG 1 cut(s) 535
BshFI GGCC 6 cut(s) 603, 641, 777, 783, 957, 984
BshNI GGYRCC 1 cut(s) 692
BsiEI CGRYCG 1 cut(s) 535
BsiHKAI GWGCWC 4 cut(s) 453, 1062, 1170, 1214
BsiSI CCGG 6 cut(s) 43, 146, 293, 696, 958, 1080
BslFI GGGAC 1 cut(s) 954
BslI CCNNNNNNNGG 6 cut(s) 145, 421, 422, 652, 689, 923
BsmFI GGGAC 1 cut(s) 954
BsmI GAATGC 1 cut(s) 702
BsnI GGCC 6 cut(s) 603, 641, 777, 783, 957, 984
Bsp1286I GDGCHC 4 cut(s) 453, 1062, 1170, 1214
Bsp13I TCCGGA 1 cut(s) 292
Bsp143I GATC 3 cut(s) 94, 553, 1369
Bsp19I CCATGG 2 cut(s) 612, 1239
Bsp68I TCGCGA 1 cut(s) 537
BspACI CCGC 5 cut(s) 124, 131, 301, 503, 1350
BspANI GGCC 6 cut(s) 603, 641, 777, 783, 957, 984
BspCNI CTCAG 1 cut(s) 1200
BspEI TCCGGA 1 cut(s) 292
BspFNI CGCG 1 cut(s) 537
BspLI GGNNCC 2 cut(s) 694, 730
BspT107I GGYRCC 1 cut(s) 692
BsrBI CCGCTC 2 cut(s) 133, 301
BsrFI RCCGGY 1 cut(s) 1079
BsrI ACTGG 3 cut(s) 577, 883, 1404
BssAI RCCGGY 1 cut(s) 1079
BssECI CCNNGG 6 cut(s) 51, 612, 662, 778, 869, 1239
BssMI GATC 3 cut(s) 94, 553, 1369
BssNAI GTATAC 2 cut(s) 215, 676
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 3 cut(s) 612, 869, 1239
Bst1107I GTATAC 2 cut(s) 215, 676
Bst2UI CCWGG 3 cut(s) 193, 377, 663
Bst4CI ACNGT 3 cut(s) 33, 994, 1223
Bst6I CTCTTC 1 cut(s) 267
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 2 cut(s) 626, 1066
BstC8I GCNNGC 1 cut(s) 1081
BstDEI CTNAG 2 cut(s) 404, 1208
BstDSI CCRYGG 3 cut(s) 612, 778, 1239
BstF5I GGATG 4 cut(s) 301, 411, 622, 1092
BstFNI CGCG 1 cut(s) 537
BstKTI GATC 3 cut(s) 97, 556, 1372
BstMBI GATC 3 cut(s) 94, 553, 1369
BstMCI CGRYCG 1 cut(s) 535
BstNI CCWGG 3 cut(s) 193, 377, 663
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 5 cut(s) 144, 191, 375, 661, 695
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 1062
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 5 cut(s) 114, 121, 700, 703, 1375
BstV2I GAAGAC 2 cut(s) 189, 491
BstXI CCANNNNNNTGG 1 cut(s) 739
BstZ17I GTATAC 2 cut(s) 215, 676
BsuRI GGCC 6 cut(s) 603, 641, 777, 783, 957, 984
BtgI CCRYGG 3 cut(s) 612, 778, 1239
BtsCI GGATG 4 cut(s) 301, 411, 622, 1092
BtuMI TCGCGA 1 cut(s) 537
Cac8I GCNNGC 1 cut(s) 1081
CaiI CAGNNNCTG 1 cut(s) 1207
Cfr10I RCCGGY 1 cut(s) 1079
Cfr13I GGNCC 3 cut(s) 602, 782, 983
CseI GACGC 1 cut(s) 951
Csp6I GTAC 8 cut(s) 100, 313, 330, 591, 767, 817, 876, 1229
CspCI CAANNNNNGTGG 2 cut(s) 934, 969
CviAII CATG 5 cut(s) 16, 398, 613, 733, 1240
CviQI GTAC 8 cut(s) 100, 313, 330, 591, 767, 817, 876, 1229
DdeI CTNAG 2 cut(s) 404, 1208
DpnI GATC 3 cut(s) 96, 555, 1371
DpnII GATC 3 cut(s) 94, 553, 1369
EaeI YGGCCR 3 cut(s) 639, 775, 955
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
EciI GGCGGA 1 cut(s) 492
Ecl136II GAGCTC 1 cut(s) 1212
Eco130I CCWWGG 3 cut(s) 612, 869, 1239
Eco24I GRGCYC 1 cut(s) 1214
Eco32I GATATC 1 cut(s) 757
Eco53kI GAGCTC 1 cut(s) 1212
Eco57I CTGAAG 2 cut(s) 1179, 1340
EcoICRI GAGCTC 1 cut(s) 1212
EcoRII CCWGG 3 cut(s) 191, 375, 661
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 3 cut(s) 612, 869, 1239
EcoT38I GRGCYC 1 cut(s) 1214
ErhI CCWWGG 3 cut(s) 612, 869, 1239
FaeI CATG 5 cut(s) 19, 401, 616, 736, 1243
FalI AAGNNNNNCTT 2 cut(s) 577, 609
FaqI GGGAC 1 cut(s) 954
FatI CATG 5 cut(s) 15, 397, 612, 732, 1239
FblI GTMKAC 3 cut(s) 168, 214, 675
Fnu4HI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
FokI GGATG 4 cut(s) 308, 398, 629, 1099
FriOI GRGCYC 1 cut(s) 1214
Fsp4HI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
FspBI CTAG 2 cut(s) 317, 1076
GluI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
GsaI CCCAGC 1 cut(s) 939
GsuI CTGGAG 2 cut(s) 359, 820
HaeIII GGCC 6 cut(s) 603, 641, 777, 783, 957, 984
HapII CCGG 6 cut(s) 43, 146, 293, 696, 958, 1080
HgaI GACGC 1 cut(s) 951
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 5 cut(s) 19, 401, 616, 736, 1243
HincII GTYRAC 3 cut(s) 169, 349, 490
HindII GTYRAC 3 cut(s) 169, 349, 490
HindIII AAGCTT 2 cut(s) 560, 668
HinfI GANTC 4 cut(s) 59, 149, 394, 522
HpaI GTTAAC 1 cut(s) 490
HpaII CCGG 6 cut(s) 43, 146, 293, 696, 958, 1080
Hpy166II GTNNAC 6 cut(s) 169, 215, 349, 490, 676, 1060
Hpy188I TCNGA 4 cut(s) 181, 1198, 1320, 1369
Hpy188III TCNNGA 7 cut(s) 293, 337, 446, 536, 634, 799, 998
Hpy8I GTNNAC 6 cut(s) 169, 215, 349, 490, 676, 1060
Hpy99I CGWCG 4 cut(s) 89, 170, 191, 203
HpyAV CCTTC 5 cut(s) 186, 217, 242, 1352, 1374
HpyCH4III ACNGT 3 cut(s) 33, 994, 1223
HpyCH4IV ACGT 3 cut(s) 186, 198, 1216
HpyCH4V TGCA 6 cut(s) 15, 620, 746, 1034, 1060, 1274
HpyF3I CTNAG 2 cut(s) 404, 1208
HpySE526I ACGT 3 cut(s) 186, 198, 1216
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 5 cut(s) 19, 401, 616, 736, 1243
Kpn2I TCCGGA 1 cut(s) 292
KroI GCCGGC 1 cut(s) 1079
KroNI GCCGGC 1 cut(s) 1081
KspAI GTTAAC 1 cut(s) 490
Kzo9I GATC 3 cut(s) 94, 553, 1369
LmnI GCTCC 5 cut(s) 138, 265, 379, 699, 728
Lsp1109I GCAGC 5 cut(s) 114, 121, 700, 703, 1375
LweI GCATC 5 cut(s) 607, 889, 1043, 1077, 1384
MaeI CTAG 2 cut(s) 317, 1076
MaeII ACGT 3 cut(s) 186, 198, 1216
MaeIII GTNAC 3 cut(s) 655, 911, 1217
MalI GATC 3 cut(s) 96, 555, 1371
MbiI CCGCTC 2 cut(s) 133, 301
MboI GATC 3 cut(s) 94, 553, 1369
MfeI CAATTG 2 cut(s) 507, 834
MhlI GDGCHC 4 cut(s) 453, 1062, 1170, 1214
MlsI TGGCCA 1 cut(s) 641
MluCI AATT 8 cut(s) 433, 507, 540, 624, 805, 834, 906, 1269
MluNI TGGCCA 1 cut(s) 641
MmeI TCCRAC 2 cut(s) 1204, 1308
Mox20I TGGCCA 1 cut(s) 641
MroI TCCGGA 1 cut(s) 292
MroNI GCCGGC 1 cut(s) 1079
MscI TGGCCA 1 cut(s) 641
MseI TTAA 6 cut(s) 342, 432, 489, 845, 1245, 1289
MslI CAYNNNNRTG 1 cut(s) 737
Msp20I TGGCCA 1 cut(s) 641
MspI CCGG 6 cut(s) 43, 146, 293, 696, 958, 1080
MspR9I CCNGG 5 cut(s) 146, 193, 377, 663, 697
MunI CAATTG 2 cut(s) 507, 834
Mva1269I GAATGC 1 cut(s) 702
MvaI CCWGG 3 cut(s) 193, 377, 663
MvnI CGCG 1 cut(s) 537
NaeI GCCGGC 1 cut(s) 1081
NciI CCSGG 2 cut(s) 146, 697
NcoI CCATGG 2 cut(s) 612, 1239
NdeII GATC 3 cut(s) 94, 553, 1369
NgoMIV GCCGGC 1 cut(s) 1079
NlaIII CATG 5 cut(s) 19, 401, 616, 736, 1243
NlaIV GGNNCC 2 cut(s) 694, 730
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 655, 911
NruI TCGCGA 1 cut(s) 537
NspI RCATGY 1 cut(s) 19
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 195, 775
PctI GAATGC 1 cut(s) 702
PdiI GCCGGC 1 cut(s) 1081
PfeI GAWTC 4 cut(s) 59, 149, 394, 522
PflMI CCANNNNNTGG 1 cut(s) 652
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 7 cut(s) 111, 126, 129, 132, 715, 718, 1390
PshBI ATTAAT 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 1214
Psp6I CCWGG 3 cut(s) 191, 375, 661
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 3 cut(s) 191, 375, 661
PspN4I GGNNCC 2 cut(s) 694, 730
PspPI GGNCC 3 cut(s) 602, 782, 983
PstNI CAGNNNCTG 1 cut(s) 1207
RruI TCGCGA 1 cut(s) 537
RsaI GTAC 8 cut(s) 101, 314, 331, 592, 768, 818, 877, 1230
RsaNI GTAC 8 cut(s) 100, 313, 330, 591, 767, 817, 876, 1229
RseI CAYNNNNRTG 1 cut(s) 737
SacI GAGCTC 1 cut(s) 1214
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 6 cut(s) 342, 432, 489, 845, 1245, 1289
SatI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
Sau3AI GATC 3 cut(s) 94, 553, 1369
Sau96I GGNCC 3 cut(s) 602, 782, 983
ScaI AGTACT 2 cut(s) 331, 1230
ScrFI CCNGG 5 cut(s) 146, 193, 377, 663, 697
SduI GDGCHC 4 cut(s) 453, 1062, 1170, 1214
SfaNI GCATC 5 cut(s) 607, 889, 1043, 1077, 1384
SfcI CTRYAG 1 cut(s) 1125
SmiMI CAYNNNNRTG 1 cut(s) 737
Sse9I AATT 8 cut(s) 433, 507, 540, 624, 805, 834, 906, 1269
SsiI CCGC 5 cut(s) 124, 131, 301, 503, 1350
SspMI CTAG 2 cut(s) 317, 1076
SstI GAGCTC 1 cut(s) 1214
StyD4I CCNGG 5 cut(s) 144, 191, 375, 661, 695
StyI CCWWGG 3 cut(s) 612, 869, 1239
TaaI ACNGT 3 cut(s) 33, 994, 1223
TaiI ACGT 3 cut(s) 189, 201, 1219
TaqI TCGA 5 cut(s) 168, 208, 427, 556, 1050
TaqII GACCGA 1 cut(s) 521
TasI AATT 8 cut(s) 433, 507, 540, 624, 805, 834, 906, 1269
TatI WGTACW 3 cut(s) 99, 329, 1228
TauI GCSGC 2 cut(s) 127, 133
TfiI GAWTC 4 cut(s) 59, 149, 394, 522
Tru1I TTAA 6 cut(s) 342, 432, 489, 845, 1245, 1289
Tru9I TTAA 6 cut(s) 342, 432, 489, 845, 1245, 1289
TseFI GTSAC 2 cut(s) 655, 911
TseI GCWGC 5 cut(s) 109, 127, 713, 716, 1388
Tsp45I GTSAC 2 cut(s) 655, 911
TspDTI ATGAA 3 cut(s) 386, 983, 1334
TspGWI ACGGA 2 cut(s) 406, 834
Van91I CCANNNNNTGG 1 cut(s) 652
VneI GTGCAC 1 cut(s) 1058
VspI ATTAAT 1 cut(s) 342
XceI RCATGY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XmiI GTMKAC 3 cut(s) 168, 214, 675
XspI CTAG 2 cut(s) 317, 1076
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 2 cut(s) 331, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.