pycom12g20900

tRNA (Guanine(10)-N2)-methyltransferase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014379
Physical Location & Seq
Reverse (-)
22241991 .. 22245187
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20900.3

Sequence Viewer

Length: 1446 bp
ATGTGGTATCTGTGCATGTTCTACCACCGACTGTTAGACTACCGGAAAGCCGAGGTGGAATCTTTAGCCCAACTCTTCGCCAACGACGAAGAAGATCAGTACAAAACGCAGCGTTTGAAATGGCGGCTGCCGCTCCACCACCATCCGGATTCCCCTTTCCATTTCGTCGACCTCCCTTCCGAAGACGTCGCCAGGAACGTGGCCAATCGAAGTATACTTGTGAAGGGAATGTATGAGCTTTGGGGAGAAGGGGGTAGCTATGAGGAGCTGGAAGAGGCTATTAGAAGTTATCCGGATGAGCGGAAGTTGCCGTACCTAGAGCCTGATAGTACTTTCAAGATTAATGTTGACACATTTGGTAAGGTCATCAGCCTCCAGGAGCAAACGCAACGGATTCATGGACTTAGCTACATCCCTTTCAAGGGTCGAGTTAATTTGAAAAGTCCAGAGCACAAGTTCTGGCTTATGGAAACTGATGATTATGGAGTTAACAATGGTCTTCCGCCAATTGTCCAAAGAAGAATCTTTTTCGGTCGCGAAATTGGTGGTGCTGATAGAAAGCTTCTACCAACATACCAGTTGAAAAGCCGTACTTATCTTGGCCCAACAGCCATGGATGCAGAAATTGCTTTCTTGATGGCCAACCAAGCATTGGTCACGCCAGGGAAGCTTGTATACGACCCTTTTGTTGGCACCGGGAGCATTCTTATAGCTGCTGCTCATTTTGGAGCCGTGACAATGGGTGCAGATATTGATATCAGGGTGGTACGAGATGGCCGTGGCCCTGATTGTAATGTCTGGAGTAATTTCAAGCAGTACGGTTTACCAATGCCAATTGCTTTGTTAAGGGCAGATAACAACCTACCTCCTTGGCGTACTGGATTGAAAGAGATCTTTGATGCCATAATTTGTGACCCTCCATATGGTGTTCGTGCTGGGGGACGCAAATCTGGTGGCCGGAAGTTGATGAAAGGGGTTGTCGGCCCTTACACCGTTCCTGATGACAAGAGGACAGACCACATACCATCAACTGCGTCTTACAGCTTGGTCGAGTGTGTGCACGACTTGCTTGATCTAGCTGGCAGGATGCTTGTGATGGGTGGGAGGCTTGTGTTCTTCTACCCTGTAGTGAGAGAAGATGATAATGTCAATAACTCTTTCCCAGAGCACCCGTGTTTCCAACTGATTGCTACTTCGGAGCAGATACTTAGCTCGCGTTACAGTAGAGTACTACTGACCATGGTTAAGACAAGTCAATACACCGAAGAAATTGCAGAAGAAGCCAGATTAAAACACAAGGACTTCAAGGAGAACCATCTGAAGTGGTTGGAAGATGGAAACCTTCATTCTGCAGTTTTCAGTCCTTCTGATCTTCCATTGAATGGTGCTGCTGATGCAAAAACCAGTAAAGAACCAAAGCCAAAATACAGAGGCAAGTATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0020

Protein Analysis

482

Amino Acids

54.66

Weight (kDa)

6.72

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26410
fragaria_vesca FvH4_6g05370
malus_domestica MD12G1223100.v1.1
prunus_persica Prupe.6G329700_v2.0.a1
pyrus_communis pycom12g20900
rosa_chinensis RchiOBHm_Chr3g0454151
rosa_laevigata RLG00000025478
rosa_multiflora Rmu_sc0004479.1_g000008 Rmu_sc0018584.1_g000001
rosa_roxburghii Rroxscaffold_165G00436930 Rroxscaffold_6G00425430
rosa_rugosa Rorug02G0653500
rosa_samantha Rh3AG056400 Rh3BG057800 Rh3CG057100 Rh3DG058300
rosa_wichuraiana Rw3G004330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AccB1I GGYRCC 1 cut(s) 692
AccB7I CCANNNNNTGG 2 cut(s) 652, 1382
AccBSI CCGCTC 2 cut(s) 133, 301
AccI GTMKAC 3 cut(s) 168, 214, 675
AccII CGCG 2 cut(s) 537, 1216
AccIII TCCGGA 2 cut(s) 145, 292
AciI CCGC 4 cut(s) 124, 131, 301, 503
AcoI YGGCCR 4 cut(s) 201, 639, 775, 955
AcuI CTGAAG 1 cut(s) 1340
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 8 cut(s) 101, 314, 331, 592, 768, 818, 877, 1230
AfiI CCNNNNNNNGG 7 cut(s) 145, 421, 422, 652, 689, 923, 1382
AgsI TTSAA 9 cut(s) 118, 337, 421, 439, 583, 811, 886, 1306, 1381
AjnI CCWGG 3 cut(s) 191, 375, 661
Alw21I GWGCWC 3 cut(s) 453, 1062, 1170
Alw44I GTGCAC 1 cut(s) 1058
Aor13HI TCCGGA 2 cut(s) 145, 292
AoxI GGCC 7 cut(s) 201, 601, 639, 775, 781, 955, 982
ApaLI GTGCAC 1 cut(s) 1058
ApeKI GCWGC 5 cut(s) 109, 127, 713, 716, 1388
AseI ATTAAT 1 cut(s) 342
AspS9I GGNCC 3 cut(s) 602, 782, 983
AsuC2I CCSGG 1 cut(s) 697
BaeGI GKGCMC 1 cut(s) 1062
BalI TGGCCA 2 cut(s) 203, 641
BanI GGYRCC 1 cut(s) 692
BarI GAAGNNNNNNTAC 4 cut(s) 296, 328, 659, 691
BbsI GAAGAC 2 cut(s) 189, 491
Bbv12I GWGCWC 3 cut(s) 453, 1062, 1170
BbvI GCAGC 5 cut(s) 114, 121, 700, 703, 1375
BccI CCATC 7 cut(s) 150, 631, 767, 1033, 1090, 1323, 1328
BceAI ACGGC 4 cut(s) 295, 573, 716, 762
BcgI CGANNNNNNTGC 2 cut(s) 1253, 1287
BciT130I CCWGG 3 cut(s) 193, 377, 663
BcnI CCSGG 1 cut(s) 697
BfaI CTAG 2 cut(s) 317, 1076
BfmI CTRYAG 2 cut(s) 1125, 1350
BglII AGATCT 1 cut(s) 891
BisI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
BlsI GCNGC 7 cut(s) 111, 126, 129, 132, 715, 718, 1390
BmcAI AGTACT 2 cut(s) 331, 1230
Bme1390I CCNGG 4 cut(s) 193, 377, 663, 697
BmgT120I GGNCC 3 cut(s) 602, 782, 983
BmiI GGNNCC 2 cut(s) 694, 730
BmrFI CCNGG 4 cut(s) 193, 377, 663, 697
BmsI GCATC 4 cut(s) 607, 889, 1077, 1384
BpiI GAAGAC 2 cut(s) 189, 491
BpmI CTGGAG 2 cut(s) 359, 820
BpuMI CCSGG 1 cut(s) 697
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 6 cut(s) 51, 612, 662, 778, 869, 1239
BsaWI WCCGGW 3 cut(s) 42, 145, 292
Bsc4I CCNNNNNNNGG 7 cut(s) 145, 421, 422, 652, 689, 923, 1382
Bse1I ACTGG 3 cut(s) 577, 883, 1404
BseAI TCCGGA 2 cut(s) 145, 292
BseBI CCWGG 3 cut(s) 193, 377, 663
BseDI CCNNGG 6 cut(s) 51, 612, 662, 778, 869, 1239
BseGI GGATG 5 cut(s) 142, 301, 411, 622, 1092
BseLI CCNNNNNNNGG 7 cut(s) 145, 421, 422, 652, 689, 923, 1382
BseNI ACTGG 3 cut(s) 577, 883, 1404
BseRI GAGGAG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 1062
BseXI GCAGC 5 cut(s) 114, 121, 700, 703, 1375
BseYI CCCAGC 1 cut(s) 935
BsgI GTGCAG 1 cut(s) 765
Bsh1236I CGCG 2 cut(s) 537, 1216
Bsh1285I CGRYCG 1 cut(s) 535
BshFI GGCC 7 cut(s) 203, 603, 641, 777, 783, 957, 984
BshNI GGYRCC 1 cut(s) 692
BsiEI CGRYCG 1 cut(s) 535
BsiHKAI GWGCWC 3 cut(s) 453, 1062, 1170
BsiSI CCGG 5 cut(s) 43, 146, 293, 696, 958
BslFI GGGAC 1 cut(s) 954
BslI CCNNNNNNNGG 7 cut(s) 145, 421, 422, 652, 689, 923, 1382
BsmFI GGGAC 1 cut(s) 954
BsmI GAATGC 1 cut(s) 702
BsnI GGCC 7 cut(s) 203, 603, 641, 777, 783, 957, 984
Bsp1286I GDGCHC 3 cut(s) 453, 1062, 1170
Bsp13I TCCGGA 2 cut(s) 145, 292
Bsp143I GATC 4 cut(s) 94, 891, 1072, 1369
Bsp19I CCATGG 2 cut(s) 612, 1239
Bsp68I TCGCGA 1 cut(s) 537
BspACI CCGC 4 cut(s) 124, 131, 301, 503
BspANI GGCC 7 cut(s) 203, 603, 641, 777, 783, 957, 984
BspEI TCCGGA 2 cut(s) 145, 292
BspFNI CGCG 2 cut(s) 537, 1216
BspLI GGNNCC 2 cut(s) 694, 730
BspMAI CTGCAG 1 cut(s) 1354
BspT107I GGYRCC 1 cut(s) 692
BsrBI CCGCTC 2 cut(s) 133, 301
BsrI ACTGG 3 cut(s) 577, 883, 1404
BssECI CCNNGG 6 cut(s) 51, 612, 662, 778, 869, 1239
BssMI GATC 4 cut(s) 94, 891, 1072, 1369
BssNAI GTATAC 2 cut(s) 215, 676
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 3 cut(s) 612, 869, 1239
Bst1107I GTATAC 2 cut(s) 215, 676
Bst2UI CCWGG 3 cut(s) 193, 377, 663
Bst4CI ACNGT 4 cut(s) 33, 821, 994, 1223
Bst6I CTCTTC 2 cut(s) 80, 267
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 2 cut(s) 626, 1066
BstC8I GCNNGC 2 cut(s) 1081, 1214
BstDEI CTNAG 2 cut(s) 404, 1208
BstDSI CCRYGG 3 cut(s) 612, 778, 1239
BstF5I GGATG 5 cut(s) 142, 301, 411, 622, 1092
BstFNI CGCG 2 cut(s) 537, 1216
BstKTI GATC 4 cut(s) 97, 894, 1075, 1372
BstMBI GATC 4 cut(s) 94, 891, 1072, 1369
BstMCI CGRYCG 1 cut(s) 535
BstNI CCWGG 3 cut(s) 193, 377, 663
BstNSI RCATGY 1 cut(s) 19
BstSCI CCNGG 4 cut(s) 191, 375, 661, 695
BstSFI CTRYAG 2 cut(s) 1125, 1350
BstSLI GKGCMC 1 cut(s) 1062
BstUI CGCG 2 cut(s) 537, 1216
BstV1I GCAGC 5 cut(s) 114, 121, 700, 703, 1375
BstV2I GAAGAC 2 cut(s) 189, 491
BstX2I RGATCY 1 cut(s) 891
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 1 cut(s) 891
BstZ17I GTATAC 2 cut(s) 215, 676
BsuRI GGCC 7 cut(s) 203, 603, 641, 777, 783, 957, 984
BtgI CCRYGG 3 cut(s) 612, 778, 1239
BtsCI GGATG 5 cut(s) 142, 301, 411, 622, 1092
BtuMI TCGCGA 1 cut(s) 537
Cac8I GCNNGC 2 cut(s) 1081, 1214
Cfr13I GGNCC 3 cut(s) 602, 782, 983
CseI GACGC 2 cut(s) 951, 1023
Csp6I GTAC 8 cut(s) 100, 313, 330, 591, 767, 817, 876, 1229
CspCI CAANNNNNGTGG 2 cut(s) 934, 969
CviAII CATG 4 cut(s) 16, 398, 613, 1240
CviQI GTAC 8 cut(s) 100, 313, 330, 591, 767, 817, 876, 1229
DdeI CTNAG 2 cut(s) 404, 1208
DpnI GATC 4 cut(s) 96, 893, 1074, 1371
DpnII GATC 4 cut(s) 94, 891, 1072, 1369
EaeI YGGCCR 4 cut(s) 201, 639, 775, 955
Eam1104I CTCTTC 2 cut(s) 80, 267
EarI CTCTTC 2 cut(s) 80, 267
EciI GGCGGA 1 cut(s) 492
Eco130I CCWWGG 3 cut(s) 612, 869, 1239
Eco32I GATATC 1 cut(s) 757
Eco57I CTGAAG 1 cut(s) 1340
EcoRII CCWGG 3 cut(s) 191, 375, 661
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 3 cut(s) 612, 869, 1239
ErhI CCWWGG 3 cut(s) 612, 869, 1239
FaeI CATG 4 cut(s) 19, 401, 616, 1243
FalI AAGNNNNNCTT 2 cut(s) 577, 609
FaqI GGGAC 1 cut(s) 954
FatI CATG 4 cut(s) 15, 397, 612, 1239
FauNDI CATATG 1 cut(s) 922
FblI GTMKAC 3 cut(s) 168, 214, 675
Fnu4HI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
FokI GGATG 5 cut(s) 129, 308, 398, 629, 1099
Fsp4HI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
FspBI CTAG 2 cut(s) 317, 1076
GluI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
GsaI CCCAGC 1 cut(s) 939
GsuI CTGGAG 2 cut(s) 359, 820
HaeIII GGCC 7 cut(s) 203, 603, 641, 777, 783, 957, 984
HapII CCGG 5 cut(s) 43, 146, 293, 696, 958
HgaI GACGC 2 cut(s) 951, 1023
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 4 cut(s) 19, 401, 616, 1243
HincII GTYRAC 3 cut(s) 169, 349, 490
HindII GTYRAC 3 cut(s) 169, 349, 490
HindIII AAGCTT 2 cut(s) 560, 668
HinfI GANTC 4 cut(s) 59, 149, 394, 522
HpaI GTTAAC 1 cut(s) 490
HpaII CCGG 5 cut(s) 43, 146, 293, 696, 958
Hpy166II GTNNAC 7 cut(s) 169, 215, 349, 490, 676, 824, 1060
Hpy188I TCNGA 4 cut(s) 181, 1198, 1320, 1369
Hpy188III TCNNGA 8 cut(s) 146, 293, 337, 446, 536, 634, 799, 998
Hpy8I GTNNAC 7 cut(s) 169, 215, 349, 490, 676, 824, 1060
Hpy99I CGWCG 3 cut(s) 89, 170, 191
HpyAV CCTTC 5 cut(s) 186, 217, 242, 1352, 1374
HpyCH4III ACNGT 4 cut(s) 33, 821, 994, 1223
HpyCH4IV ACGT 2 cut(s) 186, 198
HpyCH4V TGCA 7 cut(s) 15, 620, 746, 1060, 1274, 1352, 1397
HpyF3I CTNAG 2 cut(s) 404, 1208
HpySE526I ACGT 2 cut(s) 186, 198
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 4 cut(s) 19, 401, 616, 1243
Kpn2I TCCGGA 2 cut(s) 145, 292
KspAI GTTAAC 1 cut(s) 490
Kzo9I GATC 4 cut(s) 94, 891, 1072, 1369
LmnI GCTCC 6 cut(s) 138, 265, 379, 699, 728, 1198
Lsp1109I GCAGC 5 cut(s) 114, 121, 700, 703, 1375
LweI GCATC 4 cut(s) 607, 889, 1077, 1384
MaeI CTAG 2 cut(s) 317, 1076
MaeII ACGT 2 cut(s) 186, 198
MaeIII GTNAC 4 cut(s) 655, 733, 911, 1217
MalI GATC 4 cut(s) 96, 893, 1074, 1371
MbiI CCGCTC 2 cut(s) 133, 301
MboI GATC 4 cut(s) 94, 891, 1072, 1369
MfeI CAATTG 2 cut(s) 507, 834
MflI RGATCY 1 cut(s) 891
MhlI GDGCHC 3 cut(s) 453, 1062, 1170
MlsI TGGCCA 2 cut(s) 203, 641
MluCI AATT 8 cut(s) 433, 507, 540, 624, 805, 834, 906, 1269
MluNI TGGCCA 2 cut(s) 203, 641
MmeI TCCRAC 2 cut(s) 1204, 1308
Mox20I TGGCCA 2 cut(s) 203, 641
MroI TCCGGA 2 cut(s) 145, 292
MscI TGGCCA 2 cut(s) 203, 641
MseI TTAA 6 cut(s) 342, 432, 489, 845, 1245, 1289
Msp20I TGGCCA 2 cut(s) 203, 641
MspI CCGG 5 cut(s) 43, 146, 293, 696, 958
MspR9I CCNGG 4 cut(s) 193, 377, 663, 697
MunI CAATTG 2 cut(s) 507, 834
Mva1269I GAATGC 1 cut(s) 702
MvaI CCWGG 3 cut(s) 193, 377, 663
MvnI CGCG 2 cut(s) 537, 1216
NciI CCSGG 1 cut(s) 697
NcoI CCATGG 2 cut(s) 612, 1239
NdeI CATATG 1 cut(s) 922
NdeII GATC 4 cut(s) 94, 891, 1072, 1369
NlaIII CATG 4 cut(s) 19, 401, 616, 1243
NlaIV GGNNCC 2 cut(s) 694, 730
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 3 cut(s) 655, 733, 911
NruI TCGCGA 1 cut(s) 537
NspI RCATGY 1 cut(s) 19
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 195, 775
PctI GAATGC 1 cut(s) 702
PfeI GAWTC 4 cut(s) 59, 149, 394, 522
PflMI CCANNNNNTGG 2 cut(s) 652, 1382
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 7 cut(s) 111, 126, 129, 132, 715, 718, 1390
PshBI ATTAAT 1 cut(s) 342
Psp6I CCWGG 3 cut(s) 191, 375, 661
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 3 cut(s) 191, 375, 661
PspN4I GGNNCC 2 cut(s) 694, 730
PspPI GGNCC 3 cut(s) 602, 782, 983
PstI CTGCAG 1 cut(s) 1354
PsuI RGATCY 1 cut(s) 891
RruI TCGCGA 1 cut(s) 537
RsaI GTAC 8 cut(s) 101, 314, 331, 592, 768, 818, 877, 1230
RsaNI GTAC 8 cut(s) 100, 313, 330, 591, 767, 817, 876, 1229
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 6 cut(s) 342, 432, 489, 845, 1245, 1289
SatI GCNGC 7 cut(s) 110, 125, 128, 131, 714, 717, 1389
Sau3AI GATC 4 cut(s) 94, 891, 1072, 1369
Sau96I GGNCC 3 cut(s) 602, 782, 983
ScaI AGTACT 2 cut(s) 331, 1230
ScrFI CCNGG 4 cut(s) 193, 377, 663, 697
SduI GDGCHC 3 cut(s) 453, 1062, 1170
SfaNI GCATC 4 cut(s) 607, 889, 1077, 1384
SfcI CTRYAG 2 cut(s) 1125, 1350
Sse9I AATT 8 cut(s) 433, 507, 540, 624, 805, 834, 906, 1269
SsiI CCGC 4 cut(s) 124, 131, 301, 503
SspMI CTAG 2 cut(s) 317, 1076
StyD4I CCNGG 4 cut(s) 191, 375, 661, 695
StyI CCWWGG 3 cut(s) 612, 869, 1239
TaaI ACNGT 4 cut(s) 33, 821, 994, 1223
TaiI ACGT 2 cut(s) 189, 201
TaqI TCGA 4 cut(s) 168, 208, 427, 1050
TaqII GACCGA 1 cut(s) 521
TasI AATT 8 cut(s) 433, 507, 540, 624, 805, 834, 906, 1269
TatI WGTACW 3 cut(s) 99, 329, 1228
TauI GCSGC 2 cut(s) 127, 133
TfiI GAWTC 4 cut(s) 59, 149, 394, 522
Tru1I TTAA 6 cut(s) 342, 432, 489, 845, 1245, 1289
Tru9I TTAA 6 cut(s) 342, 432, 489, 845, 1245, 1289
TseFI GTSAC 3 cut(s) 655, 733, 911
TseI GCWGC 5 cut(s) 109, 127, 713, 716, 1388
Tsp45I GTSAC 3 cut(s) 655, 733, 911
TspDTI ATGAA 3 cut(s) 386, 983, 1334
TspGWI ACGGA 1 cut(s) 406
Van91I CCANNNNNTGG 2 cut(s) 652, 1382
VneI GTGCAC 1 cut(s) 1058
VspI ATTAAT 1 cut(s) 342
XceI RCATGY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XmiI GTMKAC 3 cut(s) 168, 214, 675
XspI CTAG 2 cut(s) 317, 1076
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 2 cut(s) 331, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.