Rh3CG057100

tRNA (Guanine(10)-N2)-methyltransferase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0014379
Physical Location & Seq
Forward (+)
3935607 .. 3939332
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG057100.1

Sequence Viewer

Length: 1218 bp
ATGTATGAACTTTGGGGAGAAGGAGGTAACTATGAGGAGCTGGAAGAGTCTATACGAAATTATCCAGATGAGCAGAAGTTGCCGTATCTAGAGTCAGACAGTACCTTCAAGATTAATGTGGATACCTTTGGTAAGGTCATCAGCCTCCAGGAGCAAACCCAACGCATTCAGGGGTTTAACTACATCCCTTTCAAGGGTCGAGTTAATTTGAGAAATCCAGAGCACAAGTTCTGGCTCATGGAAACTGATGATTATGGAGCTAATAATGGTCTTCCACCAATAGTAGAAAAAAGAATATTTTTTGGTCGAGAGATTGGTGGTGCTGATAGAAAGCTATTACCAACTTATCAACTGAAAAGCCGCACTTATCTTGGCCCAACAGCCATGGATGCAGAAATCGCATTCCTAATGGCAAACCAAGCATTAGCCACACCAGGGAAGCTTGTTTATGACCCTTTTGTTGGCACTGGGAGTATTCTTATAGCCGCTGCTCATTATGGAGCAATGACAATGGGTGCAGATATTGATATCAGGGTGGTACGCGATGGGCGCGGCCCTGACTGTAACGTTTGGAGCAATTTCAAGCAGTATGGACTACCCATGCCGATTGCTTTGCTAAGGGCAGATAATAATCTTCCTCCTTGGCGTTCTGGGCTGAAAGAGGTATTAGATGCCATAATCTGTGACCCTCCTTATGGTGTTCGTGCTGGTGGGCGCAAATCCGGCGGCCGGAAGTTGCTTAAAGGGGTTGTCGGCCCTTACATTGTGCCTGATGACAAGAGGACAGGCCACATACCATCAACTGGCGCTTACACCTTGGTCGAGTGTGTACACGATTTGCTTGACCTAGCTGCCAGGATGCTTGTAATGGGTGGAAGGCTTGTGTTCTTCTATCCTGTTTTGAGAGAAGACGATAATGCAGAAACCCATTTCCCAGAGCACCCGTGTTTCAAATTGATTGCTTCTTCTGAGCAGATTCTGAGCTCACGTTACAGTCGAGTGTTACTAACCATGGTTAAGACAAGTCAATACACTGAAGAAGTTGCAGAAGCAGCCAGAATTAAACACATCGACTTCAAGGAGAACCATGTAAAGTGGTTAGAAGATGGAAACCTTCATTCTGCAGTTTTCAGTCCCGCCGATGCTCAATTAAATGGGGCTGCTGATGCCAAAGCCACCAGAGAGGCAAAGCCAAAGTACAGGGGGAAATATGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0020

Protein Analysis

405

Amino Acids

45.35

Weight (kDa)

6.53

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmrp11_N PF25904 1 - 110 3.1e-17 Tmrp11 THUMP N-terminal domain
UPF0020 PF01170 120 - 242 2.8e-12 RMKL-like, methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26410
fragaria_vesca FvH4_6g05370
malus_domestica MD12G1223100.v1.1
prunus_persica Prupe.6G329700_v2.0.a1
pyrus_communis pycom12g20900
rosa_chinensis RchiOBHm_Chr3g0454151
rosa_laevigata RLG00000025478
rosa_multiflora Rmu_sc0004479.1_g000008 Rmu_sc0018584.1_g000001
rosa_roxburghii Rroxscaffold_165G00436930 Rroxscaffold_6G00425430
rosa_rugosa Rorug02G0653500
rosa_samantha Rh3AG056400 Rh3BG057800 Rh3CG057100 Rh3DG058300
rosa_wichuraiana Rw3G004330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 803
AccII CGCG 2 cut(s) 543, 552
AciI CCGC 5 cut(s) 361, 486, 552, 726, 1137
AclI AACGTT 1 cut(s) 567
AcoI YGGCCR 1 cut(s) 727
AcuI CTGAAG 1 cut(s) 1056
AfaI GTAC 4 cut(s) 103, 540, 831, 1199
AfiI CCNNNNNNNGG 7 cut(s) 193, 194, 435, 461, 695, 729, 803
AgsI TTSAA 5 cut(s) 109, 193, 583, 952, 1078
AjnI CCWGG 3 cut(s) 147, 433, 854
AluBI AGCT 6 cut(s) 40, 260, 334, 442, 851, 984
AluI AGCT 6 cut(s) 40, 260, 334, 442, 851, 984
Alw21I GWGCWC 3 cut(s) 225, 942, 986
AlwNI CAGNNNCTG 1 cut(s) 979
AoxI GGCC 5 cut(s) 373, 553, 727, 754, 787
ApeKI GCWGC 4 cut(s) 488, 851, 1052, 1160
AseI ATTAAT 1 cut(s) 114
AspLEI GCGC 3 cut(s) 552, 717, 809
AspS9I GGNCC 3 cut(s) 374, 554, 755
BanII GRGCYC 1 cut(s) 986
BarI GAAGNNNNNNTAC 2 cut(s) 36, 68
BbsI GAAGAC 2 cut(s) 263, 915
Bbv12I GWGCWC 3 cut(s) 225, 942, 986
BbvI GCAGC 4 cut(s) 475, 838, 1064, 1147
BccI CCATC 3 cut(s) 539, 805, 1100
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 595, 629
BciT130I CCWGG 3 cut(s) 149, 435, 856
BciVI GTATCC 1 cut(s) 115
BfaI CTAG 2 cut(s) 89, 848
BfmI CTRYAG 1 cut(s) 1122
BfoI RGCGCY 1 cut(s) 810
BfuI GTATCC 1 cut(s) 115
BisI GCNGC 8 cut(s) 361, 486, 489, 553, 727, 852, 1053, 1161
BlsI GCNGC 8 cut(s) 362, 487, 490, 554, 728, 853, 1054, 1162
Bme1390I CCNGG 3 cut(s) 149, 435, 856
BmgT120I GGNCC 3 cut(s) 374, 554, 755
BmrFI CCNGG 3 cut(s) 149, 435, 856
BmrI ACTGGG 1 cut(s) 477
BmsI GCATC 5 cut(s) 379, 661, 849, 1132, 1156
BmuI ACTGGG 1 cut(s) 477
BpiI GAAGAC 2 cut(s) 263, 915
BpmI CTGGAG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 617
BsaBI GATNNNNATC 1 cut(s) 630
BsaJI CCNNGG 5 cut(s) 384, 434, 641, 816, 1011
Bsc4I CCNNNNNNNGG 7 cut(s) 193, 194, 435, 461, 695, 729, 803
Bse1I ACTGG 2 cut(s) 472, 808
Bse3DI GCAATG 1 cut(s) 510
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 3 cut(s) 149, 435, 856
BseDI CCNNGG 5 cut(s) 384, 434, 641, 816, 1011
BseGI GGATG 3 cut(s) 183, 394, 864
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 7 cut(s) 193, 194, 435, 461, 695, 729, 803
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 2 cut(s) 960, 971
BseNI ACTGG 2 cut(s) 472, 808
BseRI GAGGAG 1 cut(s) 50
BseX3I CGGCCG 1 cut(s) 727
BseXI GCAGC 4 cut(s) 475, 838, 1064, 1147
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 2 cut(s) 543, 552
Bsh1285I CGRYCG 1 cut(s) 730
BshFI GGCC 5 cut(s) 375, 555, 729, 756, 789
BsiEI CGRYCG 1 cut(s) 730
BsiHKAI GWGCWC 3 cut(s) 225, 942, 986
BsiSI CCGG 2 cut(s) 723, 730
BslFI GGGAC 1 cut(s) 1119
BslI CCNNNNNNNGG 7 cut(s) 193, 194, 435, 461, 695, 729, 803
BsmFI GGGAC 1 cut(s) 1119
BsmI GAATGC 2 cut(s) 165, 401
BsnI GGCC 5 cut(s) 375, 555, 729, 756, 789
Bsp1286I GDGCHC 3 cut(s) 225, 942, 986
Bsp1407I TGTACA 1 cut(s) 829
Bsp19I CCATGG 2 cut(s) 384, 1011
BspACI CCGC 5 cut(s) 361, 486, 552, 726, 1137
BspANI GGCC 5 cut(s) 375, 555, 729, 756, 789
BspCNI CTCAG 2 cut(s) 961, 972
BspFNI CGCG 2 cut(s) 543, 552
BspMAI CTGCAG 1 cut(s) 1126
BsrDI GCAATG 1 cut(s) 510
BsrGI TGTACA 1 cut(s) 829
BsrI ACTGG 2 cut(s) 472, 808
BssECI CCNNGG 5 cut(s) 384, 434, 641, 816, 1011
BssT1I CCWWGG 4 cut(s) 384, 641, 816, 1011
Bst2UI CCWGG 3 cut(s) 149, 435, 856
Bst4CI ACNGT 3 cut(s) 101, 563, 995
Bst6I CTCTTC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 79
BstAUI TGTACA 1 cut(s) 829
BstDEI CTNAG 3 cut(s) 617, 969, 980
BstDSI CCRYGG 2 cut(s) 384, 1011
BstF5I GGATG 3 cut(s) 183, 394, 864
BstFNI CGCG 2 cut(s) 543, 552
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 3 cut(s) 552, 717, 809
BstMCI CGRYCG 1 cut(s) 730
BstMWI GCNNNNNNNGC 9 cut(s) 79, 389, 398, 419, 549, 652, 723, 1052, 1166
BstNI CCWGG 3 cut(s) 149, 435, 856
BstSCI CCNGG 3 cut(s) 147, 433, 854
BstSFI CTRYAG 1 cut(s) 1122
BstUI CGCG 2 cut(s) 543, 552
BstV1I GCAGC 4 cut(s) 475, 838, 1064, 1147
BstV2I GAAGAC 2 cut(s) 263, 915
BstZI CGGCCG 1 cut(s) 727
BsuI GTATCC 1 cut(s) 115
BsuRI GGCC 5 cut(s) 375, 555, 729, 756, 789
BtgI CCRYGG 2 cut(s) 384, 1011
BtgZI GCGATG 1 cut(s) 558
BtsCI GGATG 3 cut(s) 183, 394, 864
BtsIMutI CAGTG 2 cut(s) 465, 1032
CaiI CAGNNNCTG 1 cut(s) 979
CfoI GCGC 3 cut(s) 552, 717, 809
Cfr13I GGNCC 3 cut(s) 374, 554, 755
Csp6I GTAC 4 cut(s) 102, 539, 830, 1198
CviAII CATG 5 cut(s) 238, 385, 601, 1012, 1088
CviQI GTAC 4 cut(s) 102, 539, 830, 1198
DdeI CTNAG 3 cut(s) 617, 969, 980
EaeI YGGCCR 1 cut(s) 727
EagI CGGCCG 1 cut(s) 727
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Ecl136II GAGCTC 1 cut(s) 984
EclXI CGGCCG 1 cut(s) 727
Eco130I CCWWGG 4 cut(s) 384, 641, 816, 1011
Eco24I GRGCYC 1 cut(s) 986
Eco32I GATATC 1 cut(s) 529
Eco52I CGGCCG 1 cut(s) 727
Eco53kI GAGCTC 1 cut(s) 984
Eco57I CTGAAG 1 cut(s) 1056
EcoICRI GAGCTC 1 cut(s) 984
EcoRII CCWGG 3 cut(s) 147, 433, 854
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 4 cut(s) 384, 641, 816, 1011
EcoT38I GRGCYC 1 cut(s) 986
ErhI CCWWGG 4 cut(s) 384, 641, 816, 1011
FaeI CATG 5 cut(s) 241, 388, 604, 1015, 1091
FalI AAGNNNNNCTT 2 cut(s) 349, 381
FaqI GGGAC 1 cut(s) 1119
FatI CATG 5 cut(s) 237, 384, 600, 1011, 1087
FauI CCCGC 1 cut(s) 1144
Fnu4HI GCNGC 8 cut(s) 361, 486, 489, 553, 727, 852, 1053, 1161
FokI GGATG 3 cut(s) 170, 401, 871
FriOI GRGCYC 1 cut(s) 986
Fsp4HI GCNGC 8 cut(s) 361, 486, 489, 553, 727, 852, 1053, 1161
FspBI CTAG 2 cut(s) 89, 848
GlaI GCGC 3 cut(s) 551, 716, 808
GluI GCNGC 8 cut(s) 361, 486, 489, 553, 727, 852, 1053, 1161
GsuI CTGGAG 1 cut(s) 131
HaeII RGCGCY 1 cut(s) 810
HaeIII GGCC 5 cut(s) 375, 555, 729, 756, 789
HapII CCGG 2 cut(s) 723, 730
HhaI GCGC 3 cut(s) 552, 717, 809
Hin1II CATG 5 cut(s) 241, 388, 604, 1015, 1091
Hin6I GCGC 3 cut(s) 550, 715, 807
HinP1I GCGC 3 cut(s) 550, 715, 807
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 3 cut(s) 47, 92, 976
HpaII CCGG 2 cut(s) 723, 730
Hpy166II GTNNAC 2 cut(s) 830, 832
Hpy188I TCNGA 3 cut(s) 97, 970, 981
Hpy188III TCNNGA 5 cut(s) 65, 89, 109, 218, 308
Hpy8I GTNNAC 2 cut(s) 830, 832
HpyAV CCTTC 4 cut(s) 14, 115, 870, 1124
HpyCH4III ACNGT 3 cut(s) 101, 563, 995
HpyCH4IV ACGT 2 cut(s) 567, 988
HpyCH4V TGCA 5 cut(s) 392, 518, 920, 1046, 1124
HpyF10VI GCNNNNNNNGC 9 cut(s) 79, 389, 398, 419, 549, 652, 723, 1052, 1166
HpyF3I CTNAG 3 cut(s) 617, 969, 980
HpySE526I ACGT 2 cut(s) 567, 988
Hsp92II CATG 5 cut(s) 241, 388, 604, 1015, 1091
HspAI GCGC 3 cut(s) 550, 715, 807
LmnI GCTCC 5 cut(s) 37, 151, 257, 500, 573
Lsp1109I GCAGC 4 cut(s) 475, 838, 1064, 1147
LweI GCATC 5 cut(s) 379, 661, 849, 1132, 1156
MaeI CTAG 2 cut(s) 89, 848
MaeII ACGT 2 cut(s) 567, 988
MaeIII GTNAC 5 cut(s) 26, 563, 683, 989, 1002
MboII GAAGA 8 cut(s) 56, 263, 626, 880, 920, 957, 1049, 1115
MhlI GDGCHC 3 cut(s) 225, 942, 986
MluCI AATT 6 cut(s) 58, 205, 577, 953, 1059, 1148
MlyI GAGTC 2 cut(s) 56, 101
MnlI CCTC 8 cut(s) 17, 28, 155, 648, 655, 699, 774, 1177
MseI TTAA 7 cut(s) 114, 177, 204, 741, 1017, 1062, 1151
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 2 cut(s) 723, 730
MspR9I CCNGG 3 cut(s) 149, 435, 856
Mva1269I GAATGC 2 cut(s) 165, 401
MvaI CCWGG 3 cut(s) 149, 435, 856
MvnI CGCG 2 cut(s) 543, 552
MwoI GCNNNNNNNGC 9 cut(s) 79, 389, 398, 419, 549, 652, 723, 1052, 1166
NcoI CCATGG 2 cut(s) 384, 1011
NlaIII CATG 5 cut(s) 241, 388, 604, 1015, 1091
NmuCI GTSAC 1 cut(s) 683
PcsI WCGNNNNNNNCGW 1 cut(s) 994
PctI GAATGC 2 cut(s) 165, 401
PfeI GAWTC 1 cut(s) 976
PflMI CCANNNNNTGG 1 cut(s) 803
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 8 cut(s) 362, 487, 490, 554, 728, 853, 1054, 1162
PleI GAGTC 2 cut(s) 55, 100
PpsI GAGTC 2 cut(s) 55, 100
PshBI ATTAAT 1 cut(s) 114
Psp124BI GAGCTC 1 cut(s) 986
Psp1406I AACGTT 1 cut(s) 567
Psp6I CCWGG 3 cut(s) 147, 433, 854
PspGI CCWGG 3 cut(s) 147, 433, 854
PspPI GGNCC 3 cut(s) 374, 554, 755
PstI CTGCAG 1 cut(s) 1126
PstNI CAGNNNCTG 1 cut(s) 979
RsaI GTAC 4 cut(s) 103, 540, 831, 1199
RsaNI GTAC 4 cut(s) 102, 539, 830, 1198
SacI GAGCTC 1 cut(s) 986
SaqAI TTAA 7 cut(s) 114, 177, 204, 741, 1017, 1062, 1151
SatI GCNGC 8 cut(s) 361, 486, 489, 553, 727, 852, 1053, 1161
Sau96I GGNCC 3 cut(s) 374, 554, 755
SchI GAGTC 2 cut(s) 56, 101
ScrFI CCNGG 3 cut(s) 149, 435, 856
SduI GDGCHC 3 cut(s) 225, 942, 986
SfaNI GCATC 5 cut(s) 379, 661, 849, 1132, 1156
SfcI CTRYAG 1 cut(s) 1122
Sse9I AATT 6 cut(s) 58, 205, 577, 953, 1059, 1148
SsiI CCGC 5 cut(s) 361, 486, 552, 726, 1137
SspI AATATT 1 cut(s) 297
SspMI CTAG 2 cut(s) 89, 848
SstI GAGCTC 1 cut(s) 986
StyD4I CCNGG 3 cut(s) 147, 433, 854
StyI CCWWGG 4 cut(s) 384, 641, 816, 1011
TaaI ACNGT 3 cut(s) 101, 563, 995
TaiI ACGT 2 cut(s) 570, 991
TaqI TCGA 5 cut(s) 199, 307, 822, 997, 1071
TasI AATT 6 cut(s) 58, 205, 577, 953, 1059, 1148
TatI WGTACW 2 cut(s) 829, 1197
TauI GCSGC 4 cut(s) 363, 488, 555, 729
TfiI GAWTC 1 cut(s) 976
Tru1I TTAA 7 cut(s) 114, 177, 204, 741, 1017, 1062, 1151
Tru9I TTAA 7 cut(s) 114, 177, 204, 741, 1017, 1062, 1151
TscAI CASTG 2 cut(s) 472, 1039
TseFI GTSAC 1 cut(s) 683
TseI GCWGC 4 cut(s) 488, 851, 1052, 1160
Tsp45I GTSAC 1 cut(s) 683
TspDTI ATGAA 2 cut(s) 21, 1106
TspRI CASTG 2 cut(s) 472, 1039
Van91I CCANNNNNTGG 1 cut(s) 803
VspI ATTAAT 1 cut(s) 114
XbaI TCTAGA 1 cut(s) 88
XspI CTAG 2 cut(s) 89, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.