FvH4_6g05370

tRNA (Guanine(10)-N2)-methyltransferase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014379
Physical Location & Seq
Forward (+)
3022187 .. 3026063
3877 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05370.t1

Sequence Viewer

Length: 1434 bp
ATGTGGTACCTTTGCTTCTTCTTCCACAGATTACTGGACTACAGAAAAGCCGAGGTCGAATCACTAGCCCAACTCTTCTGCTCCGAAGAAGAACTTCACGATTTGCAATGGAGGCTCCCATTGCACCACCACCAAGACTCCCCCTTTCACTTCGTCAATCTCCCCTCCGAGGAAGTCGCCACCAAAATCGCCAATCGCAGCATACTAGTCAAAGGAATGTATGAACTTTGGGGAGAAGGAGGTAGCTATGAGGAGCTTGAGGAGTCTATACGAAATTACCCAGATGAGCGGAAGTTGCCGTACCTAGAGTCAGATAGTACTTTCAAGATTAATGTGGACACATTTGGAAAGGTTCTCAGCCTCCAGGAGCAAACCCAACGCATTACGGGGTTTCACTACATCCCTTTCAAGGGTCGAGTTAATTTGAGAAATCCAGAGCACAAGTTCTGGCTCATGGAAACTGATAATTATGGAGCGAACAATGGTCTTCCACCAATTGTTGAAAGAAGAATCTTTTTTGGTCGGGAGATTGGCGGTGCTGATAGAAAGCTATTGCCAACTTATGAACTGAAAAGCCGCACTTATCTGGGCCCAACAGCCATGGATGCAGAAATCGCATTCCTGATGGCGAACCAAGCATTAGCCACACCAGGGAAGCTTTTTTTCGACCCTTTTGTTGGCACTGGGAGTATTCTTATAGCCGCTGCTCATTATGGAGCAATGACAATGGGTGCAGATATTGATATCAGGGTGGTACGTGATGGGAAAGGCCCTGATTGTAACGTTTGGAGCAATTTCAAGCAGTATGGACTACCCATGCCGATTGCTTTGTTAAGGGCAGATAATAATCTTCCTCCTTGGCGTTCTGGGTTGAAAGAGGTATTTGATGCCATAATCTGTGACCCTCCTTACGGTGTTCGTGCCGGTGGGCGCAAATCCGGTGGCCGGAAGTTGCTTAAAGGGGTTGTTGGCCCTTACACTGTACCTGATGACAAGAGAACAGGCCACATACCATCAACTGGTGCTTACACCTTGGTTGAGTGCGTGCATGATTTGCTTGACCTGGCTGCCAGGATGCTTGTAATGGGTGGAAGGCTTGTGTTCTTCTATCCTGTTTTGCGAGAAGACGAAAATGCAGAAACCCATTTCCCAGAGCACCCGTGTTTCAAATTGATTGCTTCTTCTGAGCAGATTCTGAGCTCACGATATAGTCGAGTGTTACTAACCATGGTTAAGACAAGTCAATACACCGAAGAAGTTGCAGAAGCAGCCAGAATTAAGCACATCGACTTCAAAGAGAACCATCTAAAGTGGTTAGAAGATGGAAACCTTCATTCTGCAGTTTTCAGTCCTGCCGATGCTCAATTAAATGGGACCGGTGATGCTAAAGCCACCAAAGAGCAAAAGCCGAAGTACAGGGGGAAATATGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0020

Protein Analysis

478

Amino Acids

54.13

Weight (kDa)

6.48

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmrp11_N PF25904 2 - 182 1.6e-32 Tmrp11 THUMP N-terminal domain
UPF0020 PF01170 192 - 314 4.9e-13 RMKL-like, methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26410
fragaria_vesca FvH4_6g05370
malus_domestica MD12G1223100.v1.1
prunus_persica Prupe.6G329700_v2.0.a1
pyrus_communis pycom12g20900
rosa_chinensis RchiOBHm_Chr3g0454151
rosa_laevigata RLG00000025478
rosa_multiflora Rmu_sc0004479.1_g000008 Rmu_sc0018584.1_g000001
rosa_roxburghii Rroxscaffold_165G00436930 Rroxscaffold_6G00425430
rosa_rugosa Rorug02G0653500
rosa_samantha Rh3AG056400 Rh3BG057800 Rh3CG057100 Rh3DG058300
rosa_wichuraiana Rw3G004330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 6
AccB1I GGYRCC 1 cut(s) 6
AccB7I CCANNNNNTGG 1 cut(s) 1019
AccBSI CCGCTC 1 cut(s) 289
AciI CCGC 4 cut(s) 289, 534, 577, 702
AclI AACGTT 1 cut(s) 783
AcoI YGGCCR 1 cut(s) 943
AfaI GTAC 6 cut(s) 8, 302, 319, 756, 984, 1415
AfiI CCNNNNNNNGG 8 cut(s) 169, 409, 410, 651, 677, 911, 945, 1019
AgeI ACCGGT 1 cut(s) 1376
AgsI TTSAA 7 cut(s) 325, 409, 503, 799, 874, 1168, 1294
AhlI ACTAGT 1 cut(s) 205
AjnI CCWGG 4 cut(s) 363, 649, 1062, 1070
AluBI AGCT 5 cut(s) 246, 256, 550, 658, 1200
AluI AGCT 5 cut(s) 246, 256, 550, 658, 1200
Alw21I GWGCWC 3 cut(s) 441, 1158, 1202
AlwNI CAGNNNCTG 1 cut(s) 1195
AoxI GGCC 5 cut(s) 589, 769, 943, 970, 1003
ApaI GGGCCC 1 cut(s) 593
ApeKI GCWGC 4 cut(s) 198, 704, 1067, 1268
AseI ATTAAT 1 cut(s) 330
AsiGI ACCGGT 1 cut(s) 1376
Asp700I GAANNNNTTC 1 cut(s) 93
Asp718I GGTACC 1 cut(s) 6
AspLEI GCGC 1 cut(s) 933
AspS9I GGNCC 5 cut(s) 589, 590, 770, 971, 1374
AsuHPI GGTGA 1 cut(s) 1391
AvaII GGWCC 1 cut(s) 1374
BaeGI GKGCMC 1 cut(s) 593
BanI GGYRCC 1 cut(s) 6
BanII GRGCYC 2 cut(s) 593, 1202
BarI GAAGNNNNNNTAC 3 cut(s) 31, 284, 316
BbsI GAAGAC 2 cut(s) 479, 1131
Bbv12I GWGCWC 3 cut(s) 441, 1158, 1202
BbvI GCAGC 4 cut(s) 210, 691, 1054, 1280
BccI CCATC 5 cut(s) 619, 755, 1021, 1311, 1316
BceAI ACGGC 1 cut(s) 283
BcgI CGANNNNNNTGC 2 cut(s) 1241, 1275
BciT130I CCWGG 4 cut(s) 365, 651, 1064, 1072
BcuI ACTAGT 1 cut(s) 205
BfaI CTAG 3 cut(s) 65, 206, 305
BfmI CTRYAG 2 cut(s) 40, 1338
BisI GCNGC 6 cut(s) 199, 577, 702, 705, 1068, 1269
BlsI GCNGC 6 cut(s) 200, 578, 703, 706, 1069, 1270
BmcAI AGTACT 1 cut(s) 319
Bme1390I CCNGG 4 cut(s) 365, 651, 1064, 1072
Bme18I GGWCC 1 cut(s) 1374
BmgT120I GGNCC 5 cut(s) 589, 590, 770, 971, 1374
BmiI GGNNCC 4 cut(s) 8, 116, 591, 1375
BmrFI CCNGG 4 cut(s) 365, 651, 1064, 1072
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 5 cut(s) 595, 877, 1065, 1348, 1372
BmuI ACTGGG 1 cut(s) 693
BpiI GAAGAC 2 cut(s) 479, 1131
BpmI CTGGAG 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 758
BsaBI GATNNNNATC 1 cut(s) 846
BsaJI CCNNGG 7 cut(s) 51, 168, 600, 650, 857, 1032, 1227
BsaWI WCCGGW 2 cut(s) 938, 1376
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 8 cut(s) 169, 409, 410, 651, 677, 911, 945, 1019
Bse118I RCCGGY 2 cut(s) 923, 1376
Bse1I ACTGG 3 cut(s) 39, 688, 1024
Bse3DI GCAATG 3 cut(s) 113, 119, 726
Bse8I GATNNNNATC 1 cut(s) 846
BseBI CCWGG 4 cut(s) 365, 651, 1064, 1072
BseDI CCNNGG 7 cut(s) 51, 168, 600, 650, 857, 1032, 1227
BseGI GGATG 3 cut(s) 399, 610, 1080
BseJI GATNNNNATC 1 cut(s) 846
BseLI CCNNNNNNNGG 8 cut(s) 169, 409, 410, 651, 677, 911, 945, 1019
BseMI GCAATG 3 cut(s) 113, 119, 726
BseMII CTCAG 3 cut(s) 370, 1176, 1187
BseNI ACTGG 3 cut(s) 39, 688, 1024
BseRI GAGGAG 2 cut(s) 266, 275
BseSI GKGCMC 1 cut(s) 593
BseXI GCAGC 4 cut(s) 210, 691, 1054, 1280
BsgI GTGCAG 1 cut(s) 753
BshFI GGCC 5 cut(s) 591, 771, 945, 972, 1005
BshNI GGYRCC 1 cut(s) 6
BshTI ACCGGT 1 cut(s) 1376
BsiHKAI GWGCWC 3 cut(s) 441, 1158, 1202
BsiSI CCGG 4 cut(s) 924, 939, 946, 1377
BslFI GGGAC 1 cut(s) 1387
BslI CCNNNNNNNGG 8 cut(s) 169, 409, 410, 651, 677, 911, 945, 1019
BsmFI GGGAC 1 cut(s) 1387
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 5 cut(s) 591, 771, 945, 972, 1005
Bsp120I GGGCCC 1 cut(s) 589
Bsp1286I GDGCHC 4 cut(s) 441, 593, 1158, 1202
Bsp19I CCATGG 2 cut(s) 600, 1227
BspACI CCGC 4 cut(s) 289, 534, 577, 702
BspANI GGCC 5 cut(s) 591, 771, 945, 972, 1005
BspCNI CTCAG 3 cut(s) 369, 1177, 1188
BspLI GGNNCC 4 cut(s) 8, 116, 591, 1375
BspMAI CTGCAG 1 cut(s) 1342
BspT107I GGYRCC 1 cut(s) 6
BsrBI CCGCTC 1 cut(s) 289
BsrDI GCAATG 3 cut(s) 113, 119, 726
BsrFI RCCGGY 2 cut(s) 923, 1376
BsrI ACTGG 3 cut(s) 39, 688, 1024
BssAI RCCGGY 2 cut(s) 923, 1376
BssECI CCNNGG 7 cut(s) 51, 168, 600, 650, 857, 1032, 1227
BssT1I CCWWGG 4 cut(s) 600, 857, 1032, 1227
Bst2UI CCWGG 4 cut(s) 365, 651, 1064, 1072
Bst4CI ACNGT 2 cut(s) 914, 982
Bst6I CTCTTC 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 1054
BstBAI YACGTR 1 cut(s) 758
BstC8I GCNNGC 1 cut(s) 1046
BstDEI CTNAG 3 cut(s) 356, 1185, 1196
BstDSI CCRYGG 2 cut(s) 600, 1227
BstF5I GGATG 3 cut(s) 399, 610, 1080
BstHHI GCGC 1 cut(s) 933
BstMWI GCNNNNNNNGC 8 cut(s) 112, 121, 295, 605, 614, 635, 1054, 1268
BstNI CCWGG 4 cut(s) 365, 651, 1064, 1072
BstSCI CCNGG 4 cut(s) 363, 649, 1062, 1070
BstSFI CTRYAG 2 cut(s) 40, 1338
BstSLI GKGCMC 1 cut(s) 593
BstV1I GCAGC 4 cut(s) 210, 691, 1054, 1280
BstV2I GAAGAC 2 cut(s) 479, 1131
BsuRI GGCC 5 cut(s) 591, 771, 945, 972, 1005
BtgI CCRYGG 2 cut(s) 600, 1227
BtsCI GGATG 3 cut(s) 399, 610, 1080
BtsIMutI CAGTG 2 cut(s) 681, 978
Cac8I GCNNGC 1 cut(s) 1046
CaiI CAGNNNCTG 1 cut(s) 1195
CfoI GCGC 1 cut(s) 933
Cfr10I RCCGGY 2 cut(s) 923, 1376
Cfr13I GGNCC 5 cut(s) 589, 590, 770, 971, 1374
Csp6I GTAC 6 cut(s) 7, 301, 318, 755, 983, 1414
CspAI ACCGGT 1 cut(s) 1376
CspCI CAANNNNNGTGG 2 cut(s) 922, 957
CviAII CATG 5 cut(s) 454, 601, 817, 1049, 1228
CviQI GTAC 6 cut(s) 7, 301, 318, 755, 983, 1414
DdeI CTNAG 3 cut(s) 356, 1185, 1196
EaeI YGGCCR 1 cut(s) 943
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Ecl136II GAGCTC 1 cut(s) 1200
Eco130I CCWWGG 4 cut(s) 600, 857, 1032, 1227
Eco24I GRGCYC 2 cut(s) 593, 1202
Eco32I GATATC 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 1374
Eco53kI GAGCTC 1 cut(s) 1200
EcoICRI GAGCTC 1 cut(s) 1200
EcoO109I RGGNCCY 1 cut(s) 770
EcoRII CCWGG 4 cut(s) 363, 649, 1062, 1070
EcoRV GATATC 1 cut(s) 745
EcoT14I CCWWGG 4 cut(s) 600, 857, 1032, 1227
EcoT38I GRGCYC 2 cut(s) 593, 1202
ErhI CCWWGG 4 cut(s) 600, 857, 1032, 1227
FaeI CATG 5 cut(s) 457, 604, 820, 1052, 1231
FalI AAGNNNNNCTT 4 cut(s) 78, 110, 565, 597
FaqI GGGAC 1 cut(s) 1387
FatI CATG 5 cut(s) 453, 600, 816, 1048, 1227
Fnu4HI GCNGC 6 cut(s) 199, 577, 702, 705, 1068, 1269
FokI GGATG 3 cut(s) 386, 617, 1087
FriOI GRGCYC 2 cut(s) 593, 1202
Fsp4HI GCNGC 6 cut(s) 199, 577, 702, 705, 1068, 1269
FspBI CTAG 3 cut(s) 65, 206, 305
GlaI GCGC 1 cut(s) 932
GluI GCNGC 6 cut(s) 199, 577, 702, 705, 1068, 1269
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 5 cut(s) 591, 771, 945, 972, 1005
HapII CCGG 4 cut(s) 924, 939, 946, 1377
HhaI GCGC 1 cut(s) 933
Hin1II CATG 5 cut(s) 457, 604, 820, 1052, 1231
Hin6I GCGC 1 cut(s) 931
HinP1I GCGC 1 cut(s) 931
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 6 cut(s) 59, 137, 263, 308, 510, 1192
HpaII CCGG 4 cut(s) 924, 939, 946, 1377
HphI GGTGA 1 cut(s) 1391
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 5 cut(s) 85, 169, 313, 1186, 1197
Hpy188III TCNNGA 6 cut(s) 98, 325, 434, 524, 622, 1203
Hpy8I GTNNAC 1 cut(s) 337
HpyAV CCTTC 3 cut(s) 230, 1086, 1340
HpyCH4III ACNGT 2 cut(s) 914, 982
HpyCH4IV ACGT 2 cut(s) 757, 783
HpyCH4V TGCA 8 cut(s) 106, 124, 608, 734, 1048, 1136, 1262, 1340
HpyF10VI GCNNNNNNNGC 8 cut(s) 112, 121, 295, 605, 614, 635, 1054, 1268
HpyF3I CTNAG 3 cut(s) 356, 1185, 1196
HpySE526I ACGT 2 cut(s) 757, 783
Hsp92II CATG 5 cut(s) 457, 604, 820, 1052, 1231
HspAI GCGC 1 cut(s) 931
KpnI GGTACC 1 cut(s) 10
LmnI GCTCC 7 cut(s) 86, 120, 253, 367, 473, 716, 789
Lsp1109I GCAGC 4 cut(s) 210, 691, 1054, 1280
LweI GCATC 5 cut(s) 595, 877, 1065, 1348, 1372
MaeI CTAG 3 cut(s) 65, 206, 305
MaeII ACGT 2 cut(s) 757, 783
MaeIII GTNAC 3 cut(s) 779, 899, 1218
MbiI CCGCTC 1 cut(s) 289
MfeI CAATTG 1 cut(s) 495
MhlI GDGCHC 4 cut(s) 441, 593, 1158, 1202
MluCI AATT 8 cut(s) 274, 421, 466, 495, 793, 1169, 1275, 1364
MlyI GAGTC 3 cut(s) 131, 272, 317
MroXI GAANNNNTTC 1 cut(s) 93
MseI TTAA 7 cut(s) 330, 420, 833, 957, 1233, 1278, 1367
MspA1I CMGCKG 1 cut(s) 704
MspI CCGG 4 cut(s) 924, 939, 946, 1377
MspR9I CCNGG 4 cut(s) 365, 651, 1064, 1072
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 4 cut(s) 365, 651, 1064, 1072
MwoI GCNNNNNNNGC 8 cut(s) 112, 121, 295, 605, 614, 635, 1054, 1268
NcoI CCATGG 2 cut(s) 600, 1227
NlaIII CATG 5 cut(s) 457, 604, 820, 1052, 1231
NlaIV GGNNCC 4 cut(s) 8, 116, 591, 1375
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 899
PcsI WCGNNNNNNNCGW 1 cut(s) 1210
PctI GAATGC 1 cut(s) 617
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 3 cut(s) 59, 510, 1192
PflMI CCANNNNNTGG 1 cut(s) 1019
PfoI TCCNGGA 1 cut(s) 363
PinAI ACCGGT 1 cut(s) 1376
PkrI GCNGC 6 cut(s) 200, 578, 703, 706, 1069, 1270
PleI GAGTC 3 cut(s) 131, 271, 316
PpsI GAGTC 3 cut(s) 131, 271, 316
Ppu21I YACGTR 1 cut(s) 758
PshBI ATTAAT 1 cut(s) 330
Psp124BI GAGCTC 1 cut(s) 1202
Psp1406I AACGTT 1 cut(s) 783
Psp6I CCWGG 4 cut(s) 363, 649, 1062, 1070
PspGI CCWGG 4 cut(s) 363, 649, 1062, 1070
PspN4I GGNNCC 4 cut(s) 8, 116, 591, 1375
PspOMI GGGCCC 1 cut(s) 589
PspPI GGNCC 5 cut(s) 589, 590, 770, 971, 1374
PstI CTGCAG 1 cut(s) 1342
PstNI CAGNNNCTG 1 cut(s) 1195
RsaI GTAC 6 cut(s) 8, 302, 319, 756, 984, 1415
RsaNI GTAC 6 cut(s) 7, 301, 318, 755, 983, 1414
SacI GAGCTC 1 cut(s) 1202
SaqAI TTAA 7 cut(s) 330, 420, 833, 957, 1233, 1278, 1367
SatI GCNGC 6 cut(s) 199, 577, 702, 705, 1068, 1269
Sau96I GGNCC 5 cut(s) 589, 590, 770, 971, 1374
ScaI AGTACT 1 cut(s) 319
SchI GAGTC 3 cut(s) 131, 272, 317
ScrFI CCNGG 4 cut(s) 365, 651, 1064, 1072
SduI GDGCHC 4 cut(s) 441, 593, 1158, 1202
SfaNI GCATC 5 cut(s) 595, 877, 1065, 1348, 1372
SfcI CTRYAG 2 cut(s) 40, 1338
SinI GGWCC 1 cut(s) 1374
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SpeI ACTAGT 1 cut(s) 205
Sse9I AATT 8 cut(s) 274, 421, 466, 495, 793, 1169, 1275, 1364
SsiI CCGC 4 cut(s) 289, 534, 577, 702
SspMI CTAG 3 cut(s) 65, 206, 305
SstI GAGCTC 1 cut(s) 1202
StyD4I CCNGG 4 cut(s) 363, 649, 1062, 1070
StyI CCWWGG 4 cut(s) 600, 857, 1032, 1227
TaaI ACNGT 2 cut(s) 914, 982
TaiI ACGT 2 cut(s) 760, 786
TaqI TCGA 5 cut(s) 57, 415, 666, 1213, 1287
TasI AATT 8 cut(s) 274, 421, 466, 495, 793, 1169, 1275, 1364
TatI WGTACW 2 cut(s) 317, 1413
TauI GCSGC 2 cut(s) 579, 704
TfiI GAWTC 3 cut(s) 59, 510, 1192
Tru1I TTAA 7 cut(s) 330, 420, 833, 957, 1233, 1278, 1367
Tru9I TTAA 7 cut(s) 330, 420, 833, 957, 1233, 1278, 1367
TscAI CASTG 2 cut(s) 688, 985
TseFI GTSAC 1 cut(s) 899
TseI GCWGC 4 cut(s) 198, 704, 1067, 1268
Tsp45I GTSAC 1 cut(s) 899
TspDTI ATGAA 3 cut(s) 237, 579, 1322
TspRI CASTG 2 cut(s) 688, 985
Van91I CCANNNNNTGG 1 cut(s) 1019
VpaK11BI GGWCC 1 cut(s) 1374
VspI ATTAAT 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 3 cut(s) 65, 206, 305
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.