Prupe.6G329700_v2.0.a1

tRNA (Guanine(10)-N2)-methyltransferase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0014379
Physical Location & Seq
Reverse (-)
28819512 .. 28822595
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G329700.1

Sequence Viewer

Length: 1440 bp
ATGTGGTATCTGTGCGTGTTCTTCCACAGATTATTAGACTACAGGAAAGCCGAGGTCGAATCTTTAGCACAACTCTTTAGCAACGAAGATCAAGAATACCAGTGTTTGCAATGGAGGCTGCCACTGCTCCACCACCCAGATTCCCCTTTCCATTTCGTCGATCTCCCTTCGGAAGACGTAGCCAAGAGCATCGCCAGTCGAAGCATACTTGTGAAAGGAATGTATGAACTTTGGGGAGAAGGAGGTAGCTATGAGGAACTGGAAGAGTGTATAAGAAATTACCCAGATGAGCGGAAGTTGCCGTACCTAGACTCCAGTAGTACTTTCAAGATTAATGTTGACACCTTTGGTAAAGTCATCAGCCTCCAGGAGCAAACCCAACGCATTCAGGGGCTTAGCTACATTCCTTTCAAGGGTCGAGTTAATTTGAAAAATCCAGAACACAAGTTCTGGCTTATGGAAACTGATGATTATGGAGCTAGCAATGGTCTTCCACCAATGGTCCAAAAAAGAATCTTTTTCGGTCGGGAGATTGGTGGTGCTGATAGAAAACTTTTACCTACATATCAGTTGAAAAGCCGCATTTATCTTGGACCAACGGCCATGGATGCAGAAATGGCTTTCTTAATGGCAAACCAAGCTTTGGCCACACCAGGGAAGCTTGTCTACGATCCTTTTGTTGGCACTGGGAGCATTCTTATAGCTGCTGCCCATTTTGGAGCCATGACAATGGGTGCAGATATTGATATCAGGGTGGTACGCGATGGCCGTGGCCCTGACTGTAATGTCTGGAGCAATTTCAAGCAGTATGGATTACCAATGCCGATTGCTTTGTTAAGGGCAGATAATAACCTTCCTCCTTGGCGTACTGGATTGAAAGAGGTATTTGATGCCATAATTTGCGACCCCCCCTATGGTGTTCGTGCTGGGGGACGCAAATCTGGTGGCCGGAAGTTGATGAAAGGAGTTATCGGCCCTTACGTTGTTCCGGATGACAAGAGGACAGACCACATACCATCAACTGCATCTTACAGCTTGGTCGAGTGTGTACATGATTTGCTCGACCTAGCTGGCAGGATGCTTGTAATGGGTGGGAGGCTTGTGTTCTTCTACCCTGTGTTGAGTGAAGATGATAACGTCGATAACCATTTCCCAGAGCACCCGTGTTTCAAACTGATTGCTACCTCCGAGCAGATTCTGAGCTCACGTTACAGCAGAGTATTACTGACAATGGTGAAGAAAAGTCCATACACTGAAGACATCGCAGAAGCAGCCAGATTAAAACATCTAGACTTCAAAGAGAACCATCTGAAATGGTTGGAAGATGGAAACCTTCATTCTGCAGTTTTCAGTCCTACGGATCCACAATCAAATGGGGCTGCCGATCATGCGAAGTTCACCAAAGAACCAAAGCCAAAATACAGAGGAAAATATGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0020

Protein Analysis

480

Amino Acids

54.24

Weight (kDa)

6.55

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26410
fragaria_vesca FvH4_6g05370
malus_domestica MD12G1223100.v1.1
prunus_persica Prupe.6G329700_v2.0.a1
pyrus_communis pycom12g20900
rosa_chinensis RchiOBHm_Chr3g0454151
rosa_laevigata RLG00000025478
rosa_multiflora Rmu_sc0004479.1_g000008 Rmu_sc0018584.1_g000001
rosa_roxburghii Rroxscaffold_165G00436930 Rroxscaffold_6G00425430
rosa_rugosa Rorug02G0653500
rosa_samantha Rh3AG056400 Rh3BG057800 Rh3CG057100 Rh3DG058300
rosa_wichuraiana Rw3G004330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 785
AccB7I CCANNNNNTGG 1 cut(s) 643
AccBSI CCGCTC 1 cut(s) 292
AccI GTMKAC 1 cut(s) 666
AccII CGCG 1 cut(s) 762
AccIII TCCGGA 1 cut(s) 988
AciI CCGC 2 cut(s) 292, 580
AclWI GGATC 3 cut(s) 665, 1355, 1368
AcoI YGGCCR 4 cut(s) 600, 645, 766, 946
AcuI CTGAAG 1 cut(s) 1275
AfaI GTAC 5 cut(s) 305, 322, 759, 868, 1050
AfiI CCNNNNNNNGG 5 cut(s) 413, 643, 654, 680, 914
AgsI TTSAA 8 cut(s) 328, 412, 430, 574, 802, 877, 1171, 1297
AjnI CCWGG 2 cut(s) 366, 652
AluBI AGCT 9 cut(s) 249, 399, 479, 641, 661, 704, 1035, 1070, 1203
AluI AGCT 9 cut(s) 249, 399, 479, 641, 661, 704, 1035, 1070, 1203
Alw21I GWGCWC 2 cut(s) 1161, 1205
AlwI GGATC 3 cut(s) 665, 1355, 1368
AlwNI CAGNNNCTG 1 cut(s) 1198
Aor13HI TCCGGA 1 cut(s) 988
AoxI GGCC 6 cut(s) 600, 645, 766, 772, 946, 973
ApeKI GCWGC 5 cut(s) 118, 704, 707, 1271, 1379
AseI ATTAAT 1 cut(s) 333
AspS9I GGNCC 4 cut(s) 502, 593, 773, 974
AsuHPI GGTGA 2 cut(s) 1246, 1390
AsuNHI GCTAGC 1 cut(s) 479
AvaII GGWCC 2 cut(s) 502, 593
BalI TGGCCA 1 cut(s) 647
BamHI GGATCC 1 cut(s) 1360
BanII GRGCYC 1 cut(s) 1205
BarI GAAGNNNNNNTAC 4 cut(s) 287, 319, 650, 682
BbsI GAAGAC 3 cut(s) 180, 482, 1263
Bbv12I GWGCWC 2 cut(s) 1161, 1205
BbvI GCAGC 5 cut(s) 105, 691, 694, 1283, 1366
BccI CCATC 4 cut(s) 758, 1024, 1314, 1319
BceAI ACGGC 3 cut(s) 286, 615, 753
BciT130I CCWGG 2 cut(s) 368, 654
BfaI CTAG 4 cut(s) 308, 480, 1067, 1289
BfmI CTRYAG 2 cut(s) 40, 1341
BisI GCNGC 6 cut(s) 119, 580, 705, 708, 1272, 1380
BlpI GCTNAGC 1 cut(s) 395
BlsI GCNGC 6 cut(s) 120, 581, 706, 709, 1273, 1381
BmcAI AGTACT 1 cut(s) 322
Bme1390I CCNGG 2 cut(s) 368, 654
Bme18I GGWCC 2 cut(s) 502, 593
BmgT120I GGNCC 4 cut(s) 502, 593, 773, 974
BmiI GGNNCC 2 cut(s) 721, 1362
BmrFI CCNGG 2 cut(s) 368, 654
BmrI ACTGGG 1 cut(s) 696
BmsI GCATC 5 cut(s) 198, 598, 880, 1034, 1068
BmtI GCTAGC 1 cut(s) 483
BmuI ACTGGG 1 cut(s) 696
BpiI GAAGAC 3 cut(s) 180, 482, 1263
BpmI CTGGAG 3 cut(s) 298, 350, 811
Bpu1102I GCTNAGC 1 cut(s) 395
BsaJI CCNNGG 5 cut(s) 51, 603, 653, 769, 860
BsaWI WCCGGW 1 cut(s) 988
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 5 cut(s) 413, 643, 654, 680, 914
Bse1I ACTGG 6 cut(s) 100, 195, 264, 315, 691, 874
Bse3DI GCAATG 2 cut(s) 116, 490
BseAI TCCGGA 1 cut(s) 988
BseBI CCWGG 2 cut(s) 368, 654
BseDI CCNNGG 5 cut(s) 51, 603, 653, 769, 860
BseGI GGATG 3 cut(s) 613, 997, 1083
BseLI CCNNNNNNNGG 5 cut(s) 413, 643, 654, 680, 914
BseMI GCAATG 2 cut(s) 116, 490
BseMII CTCAG 1 cut(s) 1190
BseNI ACTGG 6 cut(s) 100, 195, 264, 315, 691, 874
BseXI GCAGC 5 cut(s) 105, 691, 694, 1283, 1366
BseYI CCCAGC 1 cut(s) 926
BsgI GTGCAG 1 cut(s) 756
Bsh1236I CGCG 1 cut(s) 762
Bsh1285I CGRYCG 1 cut(s) 526
BshFI GGCC 6 cut(s) 602, 647, 768, 774, 948, 975
BsiEI CGRYCG 1 cut(s) 526
BsiHKAI GWGCWC 2 cut(s) 1161, 1205
BsiSI CCGG 2 cut(s) 949, 989
BslFI GGGAC 1 cut(s) 945
BslI CCNNNNNNNGG 5 cut(s) 413, 643, 654, 680, 914
BsmFI GGGAC 1 cut(s) 945
BsmI GAATGC 2 cut(s) 384, 693
BsnI GGCC 6 cut(s) 602, 647, 768, 774, 948, 975
Bsp1286I GDGCHC 2 cut(s) 1161, 1205
Bsp13I TCCGGA 1 cut(s) 988
Bsp1407I TGTACA 1 cut(s) 1048
Bsp143I GATC 5 cut(s) 88, 160, 670, 1360, 1384
Bsp1720I GCTNAGC 1 cut(s) 395
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 2 cut(s) 292, 580
BspANI GGCC 6 cut(s) 602, 647, 768, 774, 948, 975
BspCNI CTCAG 1 cut(s) 1191
BspEI TCCGGA 1 cut(s) 988
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 2 cut(s) 721, 1362
BspMAI CTGCAG 1 cut(s) 1345
BspOI GCTAGC 1 cut(s) 483
BspPI GGATC 3 cut(s) 665, 1355, 1368
BsrBI CCGCTC 1 cut(s) 292
BsrDI GCAATG 2 cut(s) 116, 490
BsrGI TGTACA 1 cut(s) 1048
BsrI ACTGG 6 cut(s) 100, 195, 264, 315, 691, 874
BssECI CCNNGG 5 cut(s) 51, 603, 653, 769, 860
BssMI GATC 5 cut(s) 88, 160, 670, 1360, 1384
BssT1I CCWWGG 2 cut(s) 603, 860
Bst2UI CCWGG 2 cut(s) 368, 654
Bst4CI ACNGT 1 cut(s) 782
Bst6I CTCTTC 1 cut(s) 258
BstAUI TGTACA 1 cut(s) 1048
BstC8I GCNNGC 2 cut(s) 481, 1072
BstDEI CTNAG 2 cut(s) 395, 1199
BstDSI CCRYGG 2 cut(s) 603, 769
BstF5I GGATG 3 cut(s) 613, 997, 1083
BstFNI CGCG 1 cut(s) 762
BstKTI GATC 5 cut(s) 91, 163, 673, 1363, 1387
BstMBI GATC 5 cut(s) 88, 160, 670, 1360, 1384
BstMCI CGRYCG 1 cut(s) 526
BstMWI GCNNNNNNNGC 9 cut(s) 115, 124, 298, 608, 617, 638, 690, 1271, 1388
BstNI CCWGG 2 cut(s) 368, 654
BstSCI CCNGG 2 cut(s) 366, 652
BstSFI CTRYAG 2 cut(s) 40, 1341
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 5 cut(s) 105, 691, 694, 1283, 1366
BstV2I GAAGAC 3 cut(s) 180, 482, 1263
BstX2I RGATCY 1 cut(s) 1360
BstXI CCANNNNNNTGG 1 cut(s) 730
BstYI RGATCY 1 cut(s) 1360
BsuRI GGCC 6 cut(s) 602, 647, 768, 774, 948, 975
BtgI CCRYGG 2 cut(s) 603, 769
BtgZI GCGATG 3 cut(s) 175, 777, 1246
BtsCI GGATG 3 cut(s) 613, 997, 1083
BtsI GCAGTG 1 cut(s) 122
BtsIMutI CAGTG 4 cut(s) 107, 122, 684, 1251
Cac8I GCNNGC 2 cut(s) 481, 1072
CaiI CAGNNNCTG 1 cut(s) 1198
Cfr13I GGNCC 4 cut(s) 502, 593, 773, 974
CseI GACGC 1 cut(s) 942
Csp6I GTAC 5 cut(s) 304, 321, 758, 867, 1049
CspCI CAANNNNNGTGG 2 cut(s) 925, 960
CviAII CATG 4 cut(s) 604, 724, 1052, 1388
CviQI GTAC 5 cut(s) 304, 321, 758, 867, 1049
DdeI CTNAG 2 cut(s) 395, 1199
DpnI GATC 5 cut(s) 90, 162, 672, 1362, 1386
DpnII GATC 5 cut(s) 88, 160, 670, 1360, 1384
DrdI GACNNNNNNGTC 1 cut(s) 785
DseDI GACNNNNNNGTC 1 cut(s) 785
EaeI YGGCCR 4 cut(s) 600, 645, 766, 946
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 1203
Eco130I CCWWGG 2 cut(s) 603, 860
Eco24I GRGCYC 1 cut(s) 1205
Eco32I GATATC 1 cut(s) 748
Eco47I GGWCC 2 cut(s) 502, 593
Eco53kI GAGCTC 1 cut(s) 1203
Eco57I CTGAAG 1 cut(s) 1275
EcoICRI GAGCTC 1 cut(s) 1203
EcoRII CCWGG 2 cut(s) 366, 652
EcoRV GATATC 1 cut(s) 748
EcoT14I CCWWGG 2 cut(s) 603, 860
EcoT38I GRGCYC 1 cut(s) 1205
ErhI CCWWGG 2 cut(s) 603, 860
FaeI CATG 4 cut(s) 607, 727, 1055, 1391
FaqI GGGAC 1 cut(s) 945
FatI CATG 4 cut(s) 603, 723, 1051, 1387
FblI GTMKAC 1 cut(s) 666
Fnu4HI GCNGC 6 cut(s) 119, 580, 705, 708, 1272, 1380
FokI GGATG 3 cut(s) 620, 1004, 1090
FriOI GRGCYC 1 cut(s) 1205
Fsp4HI GCNGC 6 cut(s) 119, 580, 705, 708, 1272, 1380
FspBI CTAG 4 cut(s) 308, 480, 1067, 1289
GluI GCNGC 6 cut(s) 119, 580, 705, 708, 1272, 1380
GsaI CCCAGC 1 cut(s) 930
GsuI CTGGAG 3 cut(s) 298, 350, 811
HaeIII GGCC 6 cut(s) 602, 647, 768, 774, 948, 975
HapII CCGG 2 cut(s) 949, 989
HgaI GACGC 1 cut(s) 942
Hin1II CATG 4 cut(s) 607, 727, 1055, 1391
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 2 cut(s) 639, 659
HinfI GANTC 5 cut(s) 59, 140, 311, 513, 1195
HpaII CCGG 2 cut(s) 949, 989
HphI GGTGA 2 cut(s) 1246, 1390
Hpy166II GTNNAC 4 cut(s) 340, 667, 1049, 1398
Hpy188I TCNGA 4 cut(s) 172, 1189, 1200, 1311
Hpy188III TCNNGA 7 cut(s) 92, 328, 437, 527, 790, 989, 1289
Hpy8I GTNNAC 4 cut(s) 340, 667, 1049, 1398
Hpy99I CGWCG 2 cut(s) 161, 1142
HpyAV CCTTC 4 cut(s) 177, 233, 863, 1343
HpyCH4III ACNGT 1 cut(s) 782
HpyCH4IV ACGT 4 cut(s) 177, 981, 1137, 1207
HpyCH4V TGCA 5 cut(s) 109, 611, 737, 1025, 1343
HpyF10VI GCNNNNNNNGC 9 cut(s) 115, 124, 298, 608, 617, 638, 690, 1271, 1388
HpyF3I CTNAG 2 cut(s) 395, 1199
HpySE526I ACGT 4 cut(s) 177, 981, 1137, 1207
Hsp92II CATG 4 cut(s) 607, 727, 1055, 1391
Kpn2I TCCGGA 1 cut(s) 988
Kzo9I GATC 5 cut(s) 88, 160, 670, 1360, 1384
LmnI GCTCC 6 cut(s) 132, 370, 476, 690, 719, 792
Lsp1109I GCAGC 5 cut(s) 105, 691, 694, 1283, 1366
LweI GCATC 5 cut(s) 198, 598, 880, 1034, 1068
MaeI CTAG 4 cut(s) 308, 480, 1067, 1289
MaeII ACGT 4 cut(s) 177, 981, 1137, 1207
MaeIII GTNAC 1 cut(s) 1208
MalI GATC 5 cut(s) 90, 162, 672, 1362, 1386
MbiI CCGCTC 1 cut(s) 292
MboI GATC 5 cut(s) 88, 160, 670, 1360, 1384
MflI RGATCY 1 cut(s) 1360
MhlI GDGCHC 2 cut(s) 1161, 1205
MlsI TGGCCA 1 cut(s) 647
MluCI AATT 4 cut(s) 277, 424, 796, 897
MluNI TGGCCA 1 cut(s) 647
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 1299
Mox20I TGGCCA 1 cut(s) 647
MroI TCCGGA 1 cut(s) 988
MscI TGGCCA 1 cut(s) 647
MseI TTAA 5 cut(s) 333, 423, 626, 836, 1280
MslI CAYNNNNRTG 2 cut(s) 209, 728
Msp20I TGGCCA 1 cut(s) 647
MspI CCGG 2 cut(s) 949, 989
MspR9I CCNGG 2 cut(s) 368, 654
Mva1269I GAATGC 2 cut(s) 384, 693
MvaI CCWGG 2 cut(s) 368, 654
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 9 cut(s) 115, 124, 298, 608, 617, 638, 690, 1271, 1388
NcoI CCATGG 1 cut(s) 603
NdeII GATC 5 cut(s) 88, 160, 670, 1360, 1384
NheI GCTAGC 1 cut(s) 479
NlaIII CATG 4 cut(s) 607, 727, 1055, 1391
NlaIV GGNNCC 2 cut(s) 721, 1362
NmeAIII GCCGAG 1 cut(s) 76
PcsI WCGNNNNNNNCGW 2 cut(s) 766, 978
PctI GAATGC 2 cut(s) 384, 693
PfeI GAWTC 4 cut(s) 59, 140, 513, 1195
PflMI CCANNNNNTGG 1 cut(s) 643
PfoI TCCNGGA 1 cut(s) 366
PkrI GCNGC 6 cut(s) 120, 581, 706, 709, 1273, 1381
PleI GAGTC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 305
PshBI ATTAAT 1 cut(s) 333
Psp124BI GAGCTC 1 cut(s) 1205
Psp6I CCWGG 2 cut(s) 366, 652
PspFI CCCAGC 1 cut(s) 926
PspGI CCWGG 2 cut(s) 366, 652
PspN4I GGNNCC 2 cut(s) 721, 1362
PspPI GGNCC 4 cut(s) 502, 593, 773, 974
PstI CTGCAG 1 cut(s) 1345
PstNI CAGNNNCTG 1 cut(s) 1198
PsuI RGATCY 1 cut(s) 1360
RsaI GTAC 5 cut(s) 305, 322, 759, 868, 1050
RsaNI GTAC 5 cut(s) 304, 321, 758, 867, 1049
RseI CAYNNNNRTG 2 cut(s) 209, 728
SacI GAGCTC 1 cut(s) 1205
SaqAI TTAA 5 cut(s) 333, 423, 626, 836, 1280
SatI GCNGC 6 cut(s) 119, 580, 705, 708, 1272, 1380
Sau3AI GATC 5 cut(s) 88, 160, 670, 1360, 1384
Sau96I GGNCC 4 cut(s) 502, 593, 773, 974
ScaI AGTACT 1 cut(s) 322
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 2 cut(s) 368, 654
SduI GDGCHC 2 cut(s) 1161, 1205
SfaNI GCATC 5 cut(s) 198, 598, 880, 1034, 1068
SfcI CTRYAG 2 cut(s) 40, 1341
SinI GGWCC 2 cut(s) 502, 593
SmiMI CAYNNNNRTG 2 cut(s) 209, 728
Sse9I AATT 4 cut(s) 277, 424, 796, 897
SsiI CCGC 2 cut(s) 292, 580
SspMI CTAG 4 cut(s) 308, 480, 1067, 1289
SstI GAGCTC 1 cut(s) 1205
StyD4I CCNGG 2 cut(s) 366, 652
StyI CCWWGG 2 cut(s) 603, 860
TaaI ACNGT 1 cut(s) 782
TaiI ACGT 4 cut(s) 180, 984, 1140, 1210
TaqI TCGA 7 cut(s) 57, 159, 199, 418, 1041, 1062, 1140
TaqII GACCGA 1 cut(s) 512
TasI AATT 4 cut(s) 277, 424, 796, 897
TatI WGTACW 2 cut(s) 320, 1048
TauI GCSGC 1 cut(s) 582
TfiI GAWTC 4 cut(s) 59, 140, 513, 1195
Tru1I TTAA 5 cut(s) 333, 423, 626, 836, 1280
Tru9I TTAA 5 cut(s) 333, 423, 626, 836, 1280
TscAI CASTG 4 cut(s) 107, 129, 691, 1258
TseI GCWGC 5 cut(s) 118, 704, 707, 1271, 1379
TspDTI ATGAA 3 cut(s) 240, 974, 1325
TspGWI ACGGA 1 cut(s) 1373
TspRI CASTG 4 cut(s) 107, 129, 691, 1258
Van91I CCANNNNNTGG 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 502, 593
VspI ATTAAT 1 cut(s) 333
XbaI TCTAGA 1 cut(s) 1288
XmiI GTMKAC 1 cut(s) 666
XspI CTAG 4 cut(s) 308, 480, 1067, 1289
ZrmI AGTACT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.