AT3G28450
NAC Family

inactive receptor kinase At1g27190

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012859
Physical Location & Seq
Forward (+)
10667135 .. 10669428
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28450.1

Sequence Viewer

Length: 1818 bp
ATGAAAGAGATCGGCTCAAAACCAAGAAAGTTACTTCCTTTATGCTTCATCATCTTCCTCTGTTTTTGTTCCTCTGTTATGGCTGCAGATGAAGACGACATAAGATGCTTAAGAGGACTCAAAGCATCTCTCACAGATCCTCAAAACGCGTTGAAATCATGGAACTTCGACAACACAACTCTTGGGTTTCTCTGTAACTTCGTTGGTGTGTCTTGTTGGAACAATCAGGAGAATAGGGTTATCAATCTTGAGCTTCGTGATATGGGTTTATCTGGTAAAATCCCAGATTCTCTTCAATACTGTGCGAGTTTACAAAAACTTGATCTTTCTAGTAATCGATTGTCTGGTAATATCCCTACAGAGTTGTGTAATTGGTTACCTTTTTTGGTATCTCTTGATTTGTCAAACAATGAATTGAATGGTGAGATTCCTCCTGATTTAGCTAAGTGTAGCTTTGTGAATTCTTTGGTTTTGTCTGATAACCGGCTTTCGGGTCAAATCCCGGTTCAGTTCTCGGCTTTAGGGAGGTTAGGGAGGTTCTCTGTTGCTAATAATGATCTCTCAGGTCGCATTCCTGTGTTCTTTAGCTCACCGAGTTATTCCTCTGATGATTTTAGTGGGAATAAAGGTCTTTGTGGTCGTCCTTTATCTTCGAGTTGTGGTGGTTTAAGTAAGAAGAATCTTGGGATAATAATTGCAGCTGGTGTGTTTGGTGCTGCTGCATCAATGTTGTTAGCTTTTGGGATTTGGTGGTATTATCATTTGAAGTGGACAAGGAGACGAAGAAGCGGTTTAACCGAAGTAGGAGTTAGCGGTTTAGCGCAGAGACTTCGTAGTCATAAGCTTACTCAAGTGTCTTTGTTTCAGAAGCCTTTGGTTAAAGTTAAACTTGGGGATTTGATGGCTGCAACTAATAACTTCAACTCAGAGAACATTATTGTTTCCACTAGGACTGGGACTACCTATAAGGCGCTTCTTCCGGATGGTTCAGCGCTTGCGGTGAAGCATTTGAGTACGTGTAAGCTCGGGGAAAGGGAGTTTCGGTATGAGATGAATCAGCTATGGGAGCTTCGTCATTCTAACTTAGCACCGCTTCTTGGTTTCTGCGTTGTGGAAGAAGAGAAGTTTCTTGTTTACAAGTATATGTCTAATGGGACACTTCACTCGTTGTTGGATTCAAATAGGGGTGAATTAGATTGGTCGACTCGGTTTAGGATTGGTTTAGGTGCTGCAAGAGGTTTAGCTTGGCTTCACCATGGATGTCGACCTCCAATTCTACACCAAAACATTTGTTCAAGTGTGATTCTCATTGACGAGGATTTCGATGCAAGGATAATAGATTCAGGGCTTGCAAGGCTTATGGTTCCCTCGGATAACAATGAGAGTAGTTTCATGACTGGTGACTTAGGAGAGTTTGGTTATGTAGCTCCTGAATATTCTACCACAATGCTAGCATCATTAAAAGGTGATGTATATGGACTCGGTGTTGTCCTCTTGGAGTTGGCTACAGGGCTAAAAGCTGTAGGAGGTGAAGGTTTTAAAGGGAGTTTGGTGGATTGGGTGAAACAGCTTGAAAGCTCAGGTAGAATCGCTGAGACATTCGATGAAAACATTCGAGGGAAAGGACATGACGAAGAGATCTCGAAATTTGTTGAGATTGCTCTTAACTGTGTGAGTTCAAGACCTAAAGAGAGATGGTCAATGTTTCAGGCATACCAGTCACTGAAAGCTATTGCTGAGAAACAAGGCTATTCGTTCTCCGAACAAGACGACGACTTCCCTTTGATTTTCGACACGCAAGAGAACGAGAAAGTGTGA

Protein Analysis

605

Amino Acids

66.94

Weight (kDa)

6.36

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 31 - 73 2e-08 Leucine rich repeat N-terminal domain
Pkinase PF00069 317 - 573 8.4e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 329 - 572 4.9e-27 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012859)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28450
fragaria_vesca FvH4_6g49690
malus_domestica MD09G1042400.v1.1 MD17G1043700.v1.1
prunus_persica Prupe.3G275300_v2.0.a1
pyrus_communis pycom111g03320
rosa_chinensis RchiOBHm_Chr2g0169711
rosa_laevigata RLG00000021896
rosa_multiflora Rmu_sc0022598.1_g000001 Rmu_ssc0000389.1_g000032 Rmu_ssc0000389.1_g000034
rosa_roxburghii Rroxscaffold_2G00081850
rosa_rugosa Rorug02G0542400
rosa_samantha Rh2AG614800 Rh2BG626900 Rh2CG596400 Rh2DG637800
rosa_wichuraiana Rw2G050960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 834
AccI GTMKAC 2 cut(s) 1202, 1264
AccII CGCG 1 cut(s) 149
AccIII TCCGGA 1 cut(s) 979
AciI CCGC 4 cut(s) 789, 813, 998, 1091
AclWI GGATC 1 cut(s) 131
AcsI RAATTY 2 cut(s) 460, 1646
AfaI GTAC 1 cut(s) 1015
AfeI AGCGCT 1 cut(s) 993
AfiI CCNNNNNNNGG 2 cut(s) 490, 1097
AflII CTTAAG 1 cut(s) 109
AflIII ACRYGT 2 cut(s) 147, 1016
AgsI TTSAA 9 cut(s) 154, 296, 418, 766, 922, 1179, 1296, 1574, 1680
Alw26I GTCTC 3 cut(s) 772, 820, 1589
AlwI GGATC 1 cut(s) 131
AlwNI CAGNNNCTG 1 cut(s) 1723
Ama87I CYCGRG 1 cut(s) 1025
Aor13HI TCCGGA 1 cut(s) 979
Aor51HI AGCGCT 1 cut(s) 993
ApeKI GCWGC 6 cut(s) 83, 698, 716, 719, 905, 1229
ApoI RAATTY 2 cut(s) 460, 1646
ArsI GACNNNNNNTTYG 2 cut(s) 1304, 1336
Asp700I GAANNNNTTC 1 cut(s) 1611
AspLEI GCGC 3 cut(s) 823, 973, 994
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 9 cut(s) 434, 582, 1012, 1199, 1244, 1412, 1478, 1541, 1573
AsuNHI GCTAGC 1 cut(s) 1450
AvaI CYCGRG 1 cut(s) 1025
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 6 cut(s) 70, 703, 706, 710, 892, 1216
BccI CCATC 3 cut(s) 895, 977, 1689
BcgI CGANNNNNNTGC 2 cut(s) 812, 846
BcnI CCSGG 1 cut(s) 503
BcoDI GTCTC 3 cut(s) 772, 820, 1589
BfaI CTAG 3 cut(s) 330, 948, 1451
BfmI CTRYAG 4 cut(s) 84, 357, 1506, 1521
BfoI RGCGCY 2 cut(s) 974, 995
BfrI CTTAAG 1 cut(s) 109
BglII AGATCT 1 cut(s) 1638
BisI GCNGC 6 cut(s) 84, 699, 717, 720, 906, 1230
BlsI GCNGC 6 cut(s) 85, 700, 718, 721, 907, 1231
Bme1390I CCNGG 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 1025
BmiI GGNNCC 1 cut(s) 1365
BmrFI CCNGG 1 cut(s) 503
BmrI ACTGGG 1 cut(s) 963
BmsI GCATC 5 cut(s) 95, 134, 731, 1315, 1463
BmtI GCTAGC 1 cut(s) 1454
BmuI ACTGGG 1 cut(s) 963
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 1 cut(s) 31
Bpu10I CCTNAGC 1 cut(s) 1579
BpuEI CTTGAG 2 cut(s) 269, 834
BpuMI CCSGG 1 cut(s) 503
Bsa29I ATCGAT 1 cut(s) 337
BsaAI YACGTR 1 cut(s) 1017
BsaJI CCNNGG 2 cut(s) 1255, 1368
BsaWI WCCGGW 1 cut(s) 979
BsaXI ACNNNNNCTCC 2 cut(s) 1028, 1058
Bsc4I CCNNNNNNNGG 2 cut(s) 490, 1097
Bse118I RCCGGY 1 cut(s) 483
Bse1I ACTGG 3 cut(s) 958, 1402, 1717
BseAI TCCGGA 1 cut(s) 979
BseCI ATCGAT 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 1255, 1368
BseGI GGATG 2 cut(s) 988, 1265
BseLI CCNNNNNNNGG 2 cut(s) 490, 1097
BseMII CTCAG 5 cut(s) 576, 939, 1584, 1593, 1728
BseNI ACTGG 3 cut(s) 958, 1402, 1717
BseXI GCAGC 6 cut(s) 70, 703, 706, 710, 892, 1216
Bsh1236I CGCG 1 cut(s) 149
BshVI ATCGAT 1 cut(s) 337
BsiHKCI CYCGRG 1 cut(s) 1025
BsiSI CCGG 3 cut(s) 484, 503, 980
BslFI GGGAC 2 cut(s) 970, 1168
BslI CCNNNNNNNGG 2 cut(s) 490, 1097
BsmAI GTCTC 3 cut(s) 772, 820, 1589
BsmBI CGTCTC 1 cut(s) 772
BsmFI GGGAC 2 cut(s) 970, 1168
BsmI GAATGC 1 cut(s) 570
BsoBI CYCGRG 1 cut(s) 1025
Bsp13I TCCGGA 1 cut(s) 979
Bsp143I GATC 5 cut(s) 9, 136, 322, 556, 1638
Bsp19I CCATGG 1 cut(s) 1255
BspACI CCGC 4 cut(s) 789, 813, 998, 1091
BspCNI CTCAG 5 cut(s) 575, 938, 1585, 1592, 1729
BspDI ATCGAT 1 cut(s) 337
BspEI TCCGGA 1 cut(s) 979
BspFNI CGCG 1 cut(s) 149
BspHI TCATGA 1 cut(s) 1392
BspLI GGNNCC 1 cut(s) 1365
BspMAI CTGCAG 1 cut(s) 88
BspOI GCTAGC 1 cut(s) 1454
BspPI GGATC 1 cut(s) 131
BspTI CTTAAG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 483
BsrI ACTGG 3 cut(s) 958, 1402, 1717
BssAI RCCGGY 1 cut(s) 483
BssECI CCNNGG 2 cut(s) 1255, 1368
BssMI GATC 5 cut(s) 9, 136, 322, 556, 1638
BssT1I CCWWGG 1 cut(s) 1255
Bst4CI ACNGT 2 cut(s) 302, 1670
Bst6I CTCTTC 3 cut(s) 297, 1113, 1629
BstAFI CTTAAG 1 cut(s) 109
BstBAI YACGTR 1 cut(s) 1017
BstC8I GCNNGC 3 cut(s) 996, 1350, 1452
BstDEI CTNAG 8 cut(s) 444, 562, 925, 1084, 1405, 1579, 1593, 1737
BstDSI CCRYGG 1 cut(s) 1255
BstEII GGTNACC 1 cut(s) 375
BstF5I GGATG 2 cut(s) 988, 1265
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 2 cut(s) 974, 995
BstHHI GCGC 3 cut(s) 823, 973, 994
BstKTI GATC 5 cut(s) 12, 139, 325, 559, 1641
BstMAI GTCTC 3 cut(s) 772, 820, 1589
BstMBI GATC 5 cut(s) 9, 136, 322, 556, 1638
BstMWI GCNNNNNNNGC 2 cut(s) 1066, 1354
BstPI GGTNACC 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 501
BstSFI CTRYAG 4 cut(s) 84, 357, 1506, 1521
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 6 cut(s) 70, 703, 706, 710, 892, 1216
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 2 cut(s) 136, 1638
BstYI RGATCY 2 cut(s) 136, 1638
Bsu15I ATCGAT 1 cut(s) 337
BsuTUI ATCGAT 1 cut(s) 337
BtgI CCRYGG 1 cut(s) 1255
BtsCI GGATG 2 cut(s) 988, 1265
BtsIMutI CAGTG 1 cut(s) 1721
Cac8I GCNNGC 3 cut(s) 996, 1350, 1452
CaiI CAGNNNCTG 1 cut(s) 1723
CciI TCATGA 1 cut(s) 1392
CfoI GCGC 3 cut(s) 823, 973, 994
Cfr10I RCCGGY 1 cut(s) 483
ClaI ATCGAT 1 cut(s) 337
Csp6I GTAC 1 cut(s) 1014
CviAII CATG 4 cut(s) 159, 1256, 1393, 1628
CviQI GTAC 1 cut(s) 1014
DdeI CTNAG 8 cut(s) 444, 562, 925, 1084, 1405, 1579, 1593, 1737
DpnI GATC 5 cut(s) 11, 138, 324, 558, 1640
DpnII GATC 5 cut(s) 9, 136, 322, 556, 1638
DraI TTTAAA 1 cut(s) 1540
DrdI GACNNNNNNGTC 1 cut(s) 834
DseDI GACNNNNNNGTC 1 cut(s) 834
Eam1104I CTCTTC 3 cut(s) 297, 1113, 1629
EarI CTCTTC 3 cut(s) 297, 1113, 1629
Eco130I CCWWGG 1 cut(s) 1255
Eco47III AGCGCT 1 cut(s) 993
Eco88I CYCGRG 1 cut(s) 1025
Eco91I GGTNACC 1 cut(s) 375
EcoO65I GGTNACC 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 1255
ErhI CCWWGG 1 cut(s) 1255
Esp3I CGTCTC 1 cut(s) 772
FaeI CATG 4 cut(s) 162, 1259, 1396, 1631
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 873, 905
FaqI GGGAC 2 cut(s) 970, 1168
FatI CATG 4 cut(s) 158, 1255, 1392, 1627
FblI GTMKAC 2 cut(s) 1202, 1264
Fnu4HI GCNGC 6 cut(s) 84, 699, 717, 720, 906, 1230
FokI GGATG 2 cut(s) 995, 1272
Fsp4HI GCNGC 6 cut(s) 84, 699, 717, 720, 906, 1230
FspBI CTAG 3 cut(s) 330, 948, 1451
GlaI GCGC 3 cut(s) 822, 972, 993
GluI GCNGC 6 cut(s) 84, 699, 717, 720, 906, 1230
HaeII RGCGCY 2 cut(s) 974, 995
HapII CCGG 3 cut(s) 484, 503, 980
HhaI GCGC 3 cut(s) 823, 973, 994
Hin1II CATG 4 cut(s) 162, 1259, 1396, 1631
Hin6I GCGC 3 cut(s) 821, 971, 992
HinP1I GCGC 3 cut(s) 821, 971, 992
HincII GTYRAC 2 cut(s) 1203, 1265
HindII GTYRAC 2 cut(s) 1203, 1265
HindIII AAGCTT 1 cut(s) 842
HpaII CCGG 3 cut(s) 484, 503, 980
HphI GGTGA 9 cut(s) 434, 582, 1012, 1199, 1244, 1412, 1478, 1541, 1573
Hpy166II GTNNAC 5 cut(s) 311, 771, 1135, 1203, 1265
Hpy188I TCNGA 6 cut(s) 478, 607, 867, 928, 1372, 1762
Hpy8I GTNNAC 5 cut(s) 311, 771, 1135, 1203, 1265
Hpy99I CGWCG 1 cut(s) 1775
HpyAV CCTTC 1 cut(s) 1526
HpyCH4III ACNGT 2 cut(s) 302, 1670
HpyCH4IV ACGT 1 cut(s) 1016
HpyCH4V TGCA 7 cut(s) 86, 698, 722, 908, 1232, 1328, 1352
HpyF10VI GCNNNNNNNGC 2 cut(s) 1066, 1354
HpyF3I CTNAG 8 cut(s) 444, 562, 925, 1084, 1405, 1579, 1593, 1737
HpySE526I ACGT 1 cut(s) 1016
Hsp92II CATG 4 cut(s) 162, 1259, 1396, 1631
HspAI GCGC 3 cut(s) 821, 971, 992
Kpn2I TCCGGA 1 cut(s) 979
Kzo9I GATC 5 cut(s) 9, 136, 322, 556, 1638
LmnI GCTCC 2 cut(s) 1066, 1432
Lsp1109I GCAGC 6 cut(s) 70, 703, 706, 710, 892, 1216
LweI GCATC 5 cut(s) 95, 134, 731, 1315, 1463
MaeI CTAG 3 cut(s) 330, 948, 1451
MaeII ACGT 1 cut(s) 1016
MaeIII GTNAC 5 cut(s) 30, 194, 375, 1400, 1719
MalI GATC 5 cut(s) 11, 138, 324, 558, 1640
MboI GATC 5 cut(s) 9, 136, 322, 556, 1638
MflI RGATCY 2 cut(s) 136, 1638
MluCI AATT 7 cut(s) 370, 413, 460, 693, 1190, 1272, 1646
MluI ACGCGT 1 cut(s) 147
MlyI GAGTC 3 cut(s) 111, 1198, 1473
MmeI TCCRAC 2 cut(s) 197, 1152
MroI TCCGGA 1 cut(s) 979
MroXI GAANNNNTTC 1 cut(s) 1611
MseI TTAA 8 cut(s) 110, 668, 794, 879, 885, 1460, 1539, 1665
MslI CAYNNNNRTG 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 701
MspCI CTTAAG 1 cut(s) 109
MspI CCGG 3 cut(s) 484, 503, 980
MspR9I CCNGG 1 cut(s) 503
Mva1269I GAATGC 1 cut(s) 570
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 2 cut(s) 1066, 1354
NciI CCSGG 1 cut(s) 503
NcoI CCATGG 1 cut(s) 1255
NdeII GATC 5 cut(s) 9, 136, 322, 556, 1638
NheI GCTAGC 1 cut(s) 1450
NlaIII CATG 4 cut(s) 162, 1259, 1396, 1631
NlaIV GGNNCC 1 cut(s) 1365
NmeAIII GCCGAG 1 cut(s) 494
NmuCI GTSAC 2 cut(s) 1400, 1719
PagI TCATGA 1 cut(s) 1392
PcsI WCGNNNNNNNCGW 1 cut(s) 1320
PctI GAATGC 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 1611
PfeI GAWTC 8 cut(s) 287, 427, 679, 1054, 1175, 1303, 1340, 1587
PkrI GCNGC 6 cut(s) 85, 700, 718, 721, 907, 1231
PleI GAGTC 3 cut(s) 111, 1198, 1473
PpsI GAGTC 3 cut(s) 111, 1198, 1473
Ppu21I YACGTR 1 cut(s) 1017
PspEI GGTNACC 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 1365
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 1723
PsuI RGATCY 2 cut(s) 136, 1638
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 1 cut(s) 1015
RsaNI GTAC 1 cut(s) 1014
RseI CAYNNNNRTG 1 cut(s) 575
SalI GTCGAC 2 cut(s) 1201, 1263
SaqAI TTAA 8 cut(s) 110, 668, 794, 879, 885, 1460, 1539, 1665
SatI GCNGC 6 cut(s) 84, 699, 717, 720, 906, 1230
Sau3AI GATC 5 cut(s) 9, 136, 322, 556, 1638
SchI GAGTC 3 cut(s) 111, 1198, 1473
ScrFI CCNGG 1 cut(s) 503
SfaNI GCATC 5 cut(s) 95, 134, 731, 1315, 1463
SfcI CTRYAG 4 cut(s) 84, 357, 1506, 1521
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 3 cut(s) 109, 248, 849
SmoI CTYRAG 3 cut(s) 109, 248, 849
Sse9I AATT 7 cut(s) 370, 413, 460, 693, 1190, 1272, 1646
SsiI CCGC 4 cut(s) 789, 813, 998, 1091
SspI AATATT 1 cut(s) 1436
SspMI CTAG 3 cut(s) 330, 948, 1451
StyD4I CCNGG 1 cut(s) 501
StyI CCWWGG 1 cut(s) 1255
TaaI ACNGT 2 cut(s) 302, 1670
TaiI ACGT 1 cut(s) 1019
TasI AATT 7 cut(s) 370, 413, 460, 693, 1190, 1272, 1646
TfiI GAWTC 8 cut(s) 287, 427, 679, 1054, 1175, 1303, 1340, 1587
Tru1I TTAA 8 cut(s) 110, 668, 794, 879, 885, 1460, 1539, 1665
Tru9I TTAA 8 cut(s) 110, 668, 794, 879, 885, 1460, 1539, 1665
TscAI CASTG 1 cut(s) 1728
TseFI GTSAC 2 cut(s) 1400, 1719
TseI GCWGC 6 cut(s) 83, 698, 716, 719, 905, 1229
Tsp45I GTSAC 2 cut(s) 1400, 1719
TspDTI ATGAA 7 cut(s) 17, 37, 105, 426, 1067, 1381, 1620
TspRI CASTG 1 cut(s) 1728
Vha464I CTTAAG 1 cut(s) 109
XapI RAATTY 2 cut(s) 460, 1646
XmiI GTMKAC 2 cut(s) 1202, 1264
XmnI GAANNNNTTC 1 cut(s) 1611
XspI CTAG 3 cut(s) 330, 948, 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.