Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0012859

Physical Location & Seq

Genomic Coordinates Forward (+)

2747846 .. 2750245

Gene Structure 2400 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1042400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGGATTCCCGTACAGCCAGGATTTGGATTCCGGTGTTGTTTTTGAGCTGTGTGAGCACCCATTTCTGCACTTACGCCGTGGTCGAAGACGACTTGAGGTGCCTTCGGGGGCTGAAGCAGTCGCTGCATGATCCGCTCGAGAAGCTGGCGTCTTGGGATTTCGGGAACACCACCGCAGGAGTCATCTGCAAGTTCGTCGGCATCACCTGCTGGAACGATAAGGAGAATCGGATCGTCAAACTGGAGCTCCGAGAGATGGAGCTCACCGGCGCGGTTCCCAAGGAGATAGAGTACTGCGGCAGCCTCACGAATCTGGATCTCGGCAGCAACGCTATTTCCGGGCCGATTCCGCCGGATATTTGCGATTGGTTGCCCTTTTTGGTGACCCTGGATTTGTCCGGGAACGACTTCTCAGGCCCGATCCCGCCTGGCCTCCAGCACTGTAAGTATCTGAACAGTCTGATCCTTTCGGATAATAAGCTTACGGGTCCGATTCCCTTTGAATTATCTAGCTTGGGTAGGCTTAAGAAGTTTTCGGTGGCGAATAATAAGTTGAAGGGAGCTATCCCCTCGTTTCTCGACCGTTTTGATAAGGCGGATTTTGCCGGGAACAGCGGGCTTTGCGGCGGTCCTCTCGGGTCCAAGTGTGGTGGCTTGAGCAAAAAGAGTCTTGCTATTATAATTGCTGCCGGAGTTTTTGGCGCGGCTGCGTCTTTGCTGGCGGCTTTGGGGCTGTGGTGGTGGTACCATTTGAGGCTGAGTAAGCAGCGGAGGACGGGAGGTTATGGTGTTGCGAGGGAAGATTGGGCTGAAAAGTTGAGGGCTCATAGGCTTACTCAAGTTTCTTTGTTTCAGAAGCCACTTGTGAAGGTTAAGTTGGCGGATTTGATGGCGGCTACCAATAATTTTAGCCCCGAAAATGTGATCATTTCGACTAGGACGGGGACTACTTACAAGGCCCTGCTGCCTGATGGGTCGGCGCTGGCCATTAAGCGGCTTAGTGCTTGTAAGCTCGGTGAGAAGCAGTTCCGGTTGGAGATGAACCGGTTAGGACAGCTTAGGCATCCGAATTTGACCCCCCTTTTGGGTTTCTGCATTGTGGAGGAGGAGAAGCTTTTGGTGTATAAGTACTTGTCGAGTGGGACTTTGCATTCGTTGTTGCACGGAAGTGGTGAGGGATTGGATTGGCCGACTAGGTTTAGGATCGGTTTGGGTGCTGCCAGGGGACTTGCTTGGCTTCACCACGGTTGCCAACCTCCGATTATGCATCAGAACATATGCTCCAATGTGATCCTCCTCGACGAGGATTTTGATGCTAGGATAATGGATTTTGGATTGGCAACGCTCACGGCTTCTGATCCTAAGGAGAGCAGTTTCGTTAACGGAGATTTGGGAGAGCTCGGTTATGTAGCTCCAGAGTATCCGAGTACTCTGGTTGCTTCACTGAAAGGGGACGTTTATGGACTTGGAATTGTGCTTCTGGAACTGGCGACCGGGCAAATGCCTCTCGAAGTCAGCACGGCTGATGAAGGTTATAAGGGTAATGTTGTGGATTGGGTGAATCATCTATATAATTCTGGTCGAACCAAGGATGCCATCGATAAAGCTCTCATCGGAAAAGGGCATGACGAGGAAATTTTGCAGTTTCTGAAGATTGCTAGTAGTTGTGTGATTTCTCGGCCTAAGGACAGGTGGTCTATGTACCAGGTTTACCATGCATTGAAGAGTATGAGCAAAGAACACAGTTTCAGGGAACAAGACGACGAGTTTCCATTGCTCTTTCGCAAGCCAGACACGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002831 GO:0002832 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0008150 GO:0009507 GO:0009536 GO:0016020 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043901 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0065007 GO:0071944 GO:0080134 GO:1900150 GO:1900424 GO:1900425

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

601

Amino Acids

66.13

Weight (kDa)

7.2

Isoelectric Point (pI)

28.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	29 - 71	6.3e-08	Leucine rich repeat N-terminal domain
Pkinase	PF00069	314 - 572	9.7e-25	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	325 - 509	7.6e-26	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012859)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G28450
fragaria_vesca	FvH4_6g49690
malus_domestica	MD09G1042400.v1.1 MD17G1043700.v1.1
prunus_persica	Prupe.3G275300_v2.0.a1
pyrus_communis	pycom111g03320
rosa_chinensis	RchiOBHm_Chr2g0169711
rosa_laevigata	RLG00000021896
rosa_multiflora	Rmu_sc0022598.1_g000001 Rmu_ssc0000389.1_g000032 Rmu_ssc0000389.1_g000034
rosa_roxburghii	Rroxscaffold_2G00081850
rosa_rugosa	Rorug02G0542400
rosa_samantha	Rh2AG614800 Rh2BG626900 Rh2CG596400 Rh2DG637800
rosa_wichuraiana	Rw2G050960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	680, 1536
AarI	CACCTGC	1 cut(s)	215
Acc36I	ACCTGC	1 cut(s)	215
Acc65I	GGTACC	1 cut(s)	744
AccB1I	GGYRCC	2 cut(s)	99, 744
AccB7I	CCANNNNNTGG	1 cut(s)	24
AccBSI	CCGCTC	1 cut(s)	136
AccII	CGCG	2 cut(s)	272, 704
AclWI	GGATC	8 cut(s)	125, 239, 324, 415, 457, 1211, 1285, 1352
AcoI	YGGCCR	2 cut(s)	984, 1187
AcsI	RAATTY	2 cut(s)	1069, 1635
AcuI	CTGAAG	2 cut(s)	134, 1670
AcyI	GRCGYC	1 cut(s)	149
AfaI	GTAC	6 cut(s)	13, 293, 746, 1130, 1429, 1703
AfiI	CCNNNNNNNGG	6 cut(s)	24, 256, 993, 1084, 1085, 1303
AflII	CTTAAG	1 cut(s)	524
AgeI	ACCGGT	1 cut(s)	1044
AgsI	TTSAA	3 cut(s)	503, 556, 1723
AhdI	GACNNNNNGTC	1 cut(s)	1693
AjnI	CCWGG	5 cut(s)	17, 387, 427, 1220, 1704
AjuI	GAANNNNNNNTTGG	2 cut(s)	860, 892
AleI	CACNNNNGTG	1 cut(s)	1167
AloI	GAACNNNNNNTCC	2 cut(s)	1265, 1297
Alw21I	GWGCWC	4 cut(s)	59, 249, 264, 1401
AlwI	GGATC	8 cut(s)	125, 239, 324, 415, 457, 1211, 1285, 1352
AlwNI	CAGNNNCTG	2 cut(s)	124, 1648
Ama87I	CYCGRG	2 cut(s)	137, 635
AoxI	GGCC	7 cut(s)	341, 415, 430, 957, 984, 1187, 1679
ApeKI	GCWGC	8 cut(s)	124, 300, 324, 686, 707, 766, 964, 1217
ApoI	RAATTY	2 cut(s)	1069, 1635
ArsI	GACNNNNNNTTYG	8 cut(s)	376, 408, 1066, 1098, 1135, 1167, 1292, 1324
AsiGI	ACCGGT	1 cut(s)	1044
Asp700I	GAANNNNTTC	2 cut(s)	407, 1025
Asp718I	GGTACC	1 cut(s)	744
AspLEI	GCGC	3 cut(s)	272, 704, 982
AspS9I	GGNCC	6 cut(s)	341, 416, 488, 629, 639, 958
AsuC2I	CCSGG	4 cut(s)	340, 400, 607, 1495
AsuHPI	GGTGA	7 cut(s)	196, 256, 394, 1028, 1184, 1232, 1570
AvaI	CYCGRG	2 cut(s)	137, 635
AvaII	GGWCC	3 cut(s)	488, 629, 639
AxyI	CCTNAGG	2 cut(s)	1362, 1683
BalI	TGGCCA	1 cut(s)	986
BanI	GGYRCC	2 cut(s)	99, 744
BanII	GRGCYC	4 cut(s)	249, 264, 826, 1401
BbsI	GAAGAC	1 cut(s)	93
Bbv12I	GWGCWC	4 cut(s)	59, 249, 264, 1401
BbvI	GCAGC	8 cut(s)	111, 312, 336, 673, 694, 778, 951, 1204
BccI	CCATC	4 cut(s)	250, 883, 965, 1604
BceAI	ACGGC	3 cut(s)	62, 1365, 1536
BcgI	CGANNNNNNTGC	2 cut(s)	178, 212
BciT130I	CCWGG	5 cut(s)	19, 389, 429, 1222, 1706
BciVI	GTATCC	1 cut(s)	1431
BclI	TGATCA	1 cut(s)	924
BcnI	CCSGG	4 cut(s)	340, 400, 607, 1495
BfaI	CTAG	5 cut(s)	510, 936, 1194, 1317, 1659
BfoI	RGCGCY	1 cut(s)	983
BfrI	CTTAAG	1 cut(s)	524
BfuAI	ACCTGC	1 cut(s)	215
BfuI	GTATCC	1 cut(s)	1431
BmcAI	AGTACT	3 cut(s)	293, 1130, 1429
Bme1390I	CCNGG	9 cut(s)	19, 340, 389, 400, 429, 607, 1222, 1495, 1706
Bme18I	GGWCC	3 cut(s)	488, 629, 639
BmeRI	GACNNNNNGTC	1 cut(s)	1693
BmeT110I	CYCGRG	2 cut(s)	137, 635
BmgT120I	GGNCC	6 cut(s)	341, 416, 488, 629, 639, 958
BmiI	GGNNCC	5 cut(s)	101, 276, 489, 640, 746
BmrFI	CCNGG	9 cut(s)	19, 340, 389, 400, 429, 607, 1222, 1495, 1706
BmsI	GCATC	5 cut(s)	210, 1072, 1276, 1303, 1582
BpiI	GAAGAC	1 cut(s)	93
BpmI	CTGGAG	3 cut(s)	263, 419, 1398
Bpu10I	CCTNAGC	1 cut(s)	1058
BpuEI	CTTGAG	3 cut(s)	115, 676, 822
BpuMI	CCSGG	4 cut(s)	340, 400, 607, 1495
Bsa29I	ATCGAT	1 cut(s)	1599
BsaHI	GRCGYC	1 cut(s)	149
BsaJI	CCNNGG	6 cut(s)	78, 279, 387, 1221, 1243, 1587
BsaWI	WCCGGW	3 cut(s)	31, 1029, 1044
BsaXI	ACNNNNNCTCC	6 cut(s)	231, 261, 275, 305, 1265, 1295
Bsc4I	CCNNNNNNNGG	6 cut(s)	24, 256, 993, 1084, 1085, 1303
Bse118I	RCCGGY	2 cut(s)	266, 1044
Bse1I	ACTGG	2 cut(s)	246, 1491
Bse21I	CCTNAGG	2 cut(s)	1362, 1683
Bse3DI	GCAATG	1 cut(s)	1772
BseBI	CCWGG	5 cut(s)	19, 389, 429, 1222, 1706
BseCI	ATCGAT	1 cut(s)	1599
BseDI	CCNNGG	6 cut(s)	78, 279, 387, 1221, 1243, 1587
BseGI	GGATG	2 cut(s)	1063, 1597
BseLI	CCNNNNNNNGG	6 cut(s)	24, 256, 993, 1084, 1085, 1303
BseMI	GCAATG	1 cut(s)	1772
BseMII	CTCAG	2 cut(s)	426, 749
BseNI	ACTGG	2 cut(s)	246, 1491
BseRI	GAGGAG	3 cut(s)	1118, 1121, 1286
BseXI	GCAGC	8 cut(s)	111, 312, 336, 673, 694, 778, 951, 1204
BsgI	GTGCAG	1 cut(s)	52
Bsh1236I	CGCG	2 cut(s)	272, 704
Bsh1285I	CGRYCG	2 cut(s)	583, 1494
BshFI	GGCC	7 cut(s)	343, 417, 432, 959, 986, 1189, 1681
BshNI	GGYRCC	2 cut(s)	99, 744
BshTI	ACCGGT	1 cut(s)	1044
BshVI	ATCGAT	1 cut(s)	1599
BsiEI	CGRYCG	2 cut(s)	583, 1494
BsiHKAI	GWGCWC	4 cut(s)	59, 249, 264, 1401
BsiHKCI	CYCGRG	2 cut(s)	137, 635
BslFI	GGGAC	4 cut(s)	958, 1156, 1239, 1466
BslI	CCNNNNNNNGG	6 cut(s)	24, 256, 993, 1084, 1085, 1303
BsmFI	GGGAC	4 cut(s)	958, 1156, 1239, 1466
BsmI	GAATGC	1 cut(s)	1150
BsnI	GGCC	7 cut(s)	343, 417, 432, 959, 986, 1189, 1681
BsoBI	CYCGRG	2 cut(s)	137, 635
Bsp1286I	GDGCHC	5 cut(s)	59, 249, 264, 826, 1401
Bsp143I	GATC	9 cut(s)	130, 231, 316, 420, 462, 924, 1203, 1290, 1357
BspANI	GGCC	7 cut(s)	343, 417, 432, 959, 986, 1189, 1681
BspCNI	CTCAG	2 cut(s)	425, 750
BspDI	ATCGAT	1 cut(s)	1599
BspFNI	CGCG	2 cut(s)	272, 704
BspLI	GGNNCC	5 cut(s)	101, 276, 489, 640, 746
BspMI	ACCTGC	1 cut(s)	215
BspPI	GGATC	8 cut(s)	125, 239, 324, 415, 457, 1211, 1285, 1352
BspT107I	GGYRCC	2 cut(s)	99, 744
BspTI	CTTAAG	1 cut(s)	524
BsrBI	CCGCTC	1 cut(s)	136
BsrDI	GCAATG	1 cut(s)	1772
BsrFI	RCCGGY	2 cut(s)	266, 1044
BsrI	ACTGG	2 cut(s)	246, 1491
BssAI	RCCGGY	2 cut(s)	266, 1044
BssECI	CCNNGG	6 cut(s)	78, 279, 387, 1221, 1243, 1587
BssMI	GATC	9 cut(s)	130, 231, 316, 420, 462, 924, 1203, 1290, 1357
BssNI	GRCGYC	1 cut(s)	149
BssT1I	CCWWGG	2 cut(s)	279, 1587
Bst2UI	CCWGG	5 cut(s)	19, 389, 429, 1222, 1706
Bst4CI	ACNGT	5 cut(s)	443, 458, 584, 1247, 1745
Bst6I	CTCTTC	1 cut(s)	1718
BstACI	GRCGYC	1 cut(s)	149
BstAFI	CTTAAG	1 cut(s)	524
BstAPI	GCANNNNNTGC	2 cut(s)	124, 207
BstC8I	GCNNGC	5 cut(s)	147, 617, 720, 984, 1787
BstDEI	CTNAG	6 cut(s)	412, 758, 998, 1058, 1362, 1683
BstDSI	CCRYGG	2 cut(s)	78, 1243
BstEII	GGTNACC	1 cut(s)	382
BstENI	CCTNNNNNAGG	1 cut(s)	1301
BstF5I	GGATG	2 cut(s)	1063, 1597
BstFNI	CGCG	2 cut(s)	272, 704
BstH2I	RGCGCY	1 cut(s)	983
BstHHI	GCGC	3 cut(s)	272, 704, 982
BstKTI	GATC	9 cut(s)	133, 234, 319, 423, 465, 927, 1206, 1293, 1360
BstMBI	GATC	9 cut(s)	130, 231, 316, 420, 462, 924, 1203, 1290, 1357
BstMCI	CGRYCG	2 cut(s)	583, 1494
BstMWI	GCNNNNNNNGC	9 cut(s)	54, 124, 133, 142, 207, 349, 602, 621, 763
BstNI	CCWGG	5 cut(s)	19, 389, 429, 1222, 1706
BstPI	GGTNACC	1 cut(s)	382
BstSCI	CCNGG	9 cut(s)	17, 338, 387, 398, 427, 605, 1220, 1493, 1704
BstUI	CGCG	2 cut(s)	272, 704
BstV1I	GCAGC	8 cut(s)	111, 312, 336, 673, 694, 778, 951, 1204
BstV2I	GAAGAC	1 cut(s)	93
BstX2I	RGATCY	1 cut(s)	316
BstYI	RGATCY	1 cut(s)	316
Bsu15I	ATCGAT	1 cut(s)	1599
Bsu36I	CCTNAGG	2 cut(s)	1362, 1683
BsuI	GTATCC	1 cut(s)	1431
BsuRI	GGCC	7 cut(s)	343, 417, 432, 959, 986, 1189, 1681
BsuTUI	ATCGAT	1 cut(s)	1599
BtgI	CCRYGG	2 cut(s)	78, 1243
BtsCI	GGATG	2 cut(s)	1063, 1597
BtsIMutI	CAGTG	2 cut(s)	439, 1442
BveI	ACCTGC	1 cut(s)	215
Cac8I	GCNNGC	5 cut(s)	147, 617, 720, 984, 1787
CaiI	CAGNNNCTG	2 cut(s)	124, 1648
CfoI	GCGC	3 cut(s)	272, 704, 982
Cfr10I	RCCGGY	2 cut(s)	266, 1044
Cfr13I	GGNCC	6 cut(s)	341, 416, 488, 629, 639, 958
ClaI	ATCGAT	1 cut(s)	1599
CseI	GACGC	2 cut(s)	138, 699
CsiI	ACCWGGT	1 cut(s)	1704
Csp6I	GTAC	6 cut(s)	12, 292, 745, 1129, 1428, 1702
CspAI	ACCGGT	1 cut(s)	1044
CspCI	CAANNNNNGTGG	2 cut(s)	631, 666
CviAII	CATG	3 cut(s)	128, 1625, 1715
CviQI	GTAC	6 cut(s)	12, 292, 745, 1129, 1428, 1702
DdeI	CTNAG	6 cut(s)	412, 758, 998, 1058, 1362, 1683
DpnI	GATC	9 cut(s)	132, 233, 318, 422, 464, 926, 1205, 1292, 1359
DpnII	GATC	9 cut(s)	130, 231, 316, 420, 462, 924, 1203, 1290, 1357
DriI	GACNNNNNGTC	1 cut(s)	1693
EaeI	YGGCCR	2 cut(s)	984, 1187
Eam1104I	CTCTTC	1 cut(s)	1718
Eam1105I	GACNNNNNGTC	1 cut(s)	1693
EarI	CTCTTC	1 cut(s)	1718
EciI	GGCGGA	3 cut(s)	339, 611, 896
Ecl136II	GAGCTC	3 cut(s)	247, 262, 1399
Eco130I	CCWWGG	2 cut(s)	279, 1587
Eco24I	GRGCYC	4 cut(s)	249, 264, 826, 1401
Eco47I	GGWCC	3 cut(s)	488, 629, 639
Eco53kI	GAGCTC	3 cut(s)	247, 262, 1399
Eco57I	CTGAAG	2 cut(s)	134, 1670
Eco81I	CCTNAGG	2 cut(s)	1362, 1683
Eco88I	CYCGRG	2 cut(s)	137, 635
Eco91I	GGTNACC	1 cut(s)	382
EcoICRI	GAGCTC	3 cut(s)	247, 262, 1399
EcoNI	CCTNNNNNAGG	1 cut(s)	1301
EcoO109I	RGGNCCY	1 cut(s)	958
EcoO65I	GGTNACC	1 cut(s)	382
EcoRII	CCWGG	5 cut(s)	17, 387, 427, 1220, 1704
EcoT14I	CCWWGG	2 cut(s)	279, 1587
EcoT22I	ATGCAT	2 cut(s)	1269, 1720
EcoT38I	GRGCYC	4 cut(s)	249, 264, 826, 1401
ErhI	CCWWGG	2 cut(s)	279, 1587
FaeI	CATG	3 cut(s)	131, 1628, 1718
FaqI	GGGAC	4 cut(s)	958, 1156, 1239, 1466
FatI	CATG	3 cut(s)	127, 1624, 1714
FauI	CCCGC	2 cut(s)	432, 608
FauNDI	CATATG	1 cut(s)	1277
FbaI	TGATCA	1 cut(s)	924
FokI	GGATG	2 cut(s)	1050, 1604
FriOI	GRGCYC	4 cut(s)	249, 264, 826, 1401
FspBI	CTAG	5 cut(s)	510, 936, 1194, 1317, 1659
GlaI	GCGC	3 cut(s)	271, 703, 981
GsuI	CTGGAG	3 cut(s)	263, 419, 1398
HaeII	RGCGCY	1 cut(s)	983
HaeIII	GGCC	7 cut(s)	343, 417, 432, 959, 986, 1189, 1681
HgaI	GACGC	2 cut(s)	138, 699
HhaI	GCGC	3 cut(s)	272, 704, 982
Hin1I	GRCGYC	1 cut(s)	149
Hin1II	CATG	3 cut(s)	131, 1628, 1718
Hin6I	GCGC	3 cut(s)	270, 702, 980
HinP1I	GCGC	3 cut(s)	270, 702, 980
HincII	GTYRAC	1 cut(s)	1381
HindII	GTYRAC	1 cut(s)	1381
HindIII	AAGCTT	2 cut(s)	479, 1112
HinfI	GANTC	9 cut(s)	5, 28, 180, 226, 310, 346, 493, 667, 1561
HpaI	GTTAAC	1 cut(s)	1381
HphI	GGTGA	7 cut(s)	196, 256, 394, 1028, 1184, 1232, 1570
Hpy166II	GTNNAC	2 cut(s)	1381, 1711
Hpy188III	TCNNGA	8 cut(s)	139, 163, 307, 314, 578, 1415, 1481, 1508
Hpy8I	GTNNAC	2 cut(s)	1381, 1711
Hpy99I	CGWCG	3 cut(s)	200, 1304, 1766
HpyAV	CCTTC	4 cut(s)	113, 550, 862, 1523
HpyCH4III	ACNGT	5 cut(s)	443, 458, 584, 1247, 1745
HpyCH4IV	ACGT	1 cut(s)	1455
HpyCH4V	TGCA	9 cut(s)	69, 127, 189, 1095, 1150, 1162, 1267, 1642, 1718
HpyF10VI	GCNNNNNNNGC	9 cut(s)	54, 124, 133, 142, 207, 349, 602, 621, 763
HpyF3I	CTNAG	6 cut(s)	412, 758, 998, 1058, 1362, 1683
HpySE526I	ACGT	1 cut(s)	1455
Hsp92I	GRCGYC	1 cut(s)	149
Hsp92II	CATG	3 cut(s)	131, 1628, 1718
HspAI	GCGC	3 cut(s)	270, 702, 980
KpnI	GGTACC	1 cut(s)	748
Ksp22I	TGATCA	1 cut(s)	924
KspAI	GTTAAC	1 cut(s)	1381
Kzo9I	GATC	9 cut(s)	130, 231, 316, 420, 462, 924, 1203, 1290, 1357
LmnI	GCTCC	6 cut(s)	244, 252, 259, 560, 1286, 1417
Lsp1109I	GCAGC	8 cut(s)	111, 312, 336, 673, 694, 778, 951, 1204
LweI	GCATC	5 cut(s)	210, 1072, 1276, 1303, 1582
MabI	ACCWGGT	1 cut(s)	1704
MaeI	CTAG	5 cut(s)	510, 936, 1194, 1317, 1659
MaeII	ACGT	1 cut(s)	1455
MaeIII	GTNAC	1 cut(s)	382
MalI	GATC	9 cut(s)	132, 233, 318, 422, 464, 926, 1205, 1292, 1359
MbiI	CCGCTC	1 cut(s)	136
MboI	GATC	9 cut(s)	130, 231, 316, 420, 462, 924, 1203, 1290, 1357
MboII	GAAGA	4 cut(s)	98, 812, 1663, 1735
MflI	RGATCY	1 cut(s)	316
MhlI	GDGCHC	5 cut(s)	59, 249, 264, 826, 1401
MlsI	TGGCCA	1 cut(s)	986
MluCI	AATT	7 cut(s)	503, 681, 904, 1069, 1470, 1573, 1635
MluNI	TGGCCA	1 cut(s)	986
MlyI	GAGTC	2 cut(s)	189, 676
MmeI	TCCRAC	1 cut(s)	1014
Mox20I	TGGCCA	1 cut(s)	986
Mph1103I	ATGCAT	2 cut(s)	1269, 1720
MroXI	GAANNNNTTC	2 cut(s)	407, 1025
MscI	TGGCCA	1 cut(s)	986
MseI	TTAA	4 cut(s)	525, 873, 990, 1380
MslI	CAYNNNNRTG	1 cut(s)	1167
Msp20I	TGGCCA	1 cut(s)	986
MspA1I	CMGCKG	2 cut(s)	615, 769
MspCI	CTTAAG	1 cut(s)	524
MspR9I	CCNGG	9 cut(s)	19, 340, 389, 400, 429, 607, 1222, 1495, 1706
Mva1269I	GAATGC	1 cut(s)	1150
MvaI	CCWGG	5 cut(s)	19, 389, 429, 1222, 1706
MvnI	CGCG	2 cut(s)	272, 704
MwoI	GCNNNNNNNGC	9 cut(s)	54, 124, 133, 142, 207, 349, 602, 621, 763
NciI	CCSGG	4 cut(s)	340, 400, 607, 1495
NdeI	CATATG	1 cut(s)	1277
NdeII	GATC	9 cut(s)	130, 231, 316, 420, 462, 924, 1203, 1290, 1357
NlaIII	CATG	3 cut(s)	131, 1628, 1718
NlaIV	GGNNCC	5 cut(s)	101, 276, 489, 640, 746
NmeAIII	GCCGAG	2 cut(s)	300, 1657
NmuCI	GTSAC	1 cut(s)	382
NsiI	ATGCAT	2 cut(s)	1269, 1720
OliI	CACNNNNGTG	1 cut(s)	1167
PaeR7I	CTCGAG	1 cut(s)	137
PaqCI	CACCTGC	1 cut(s)	215
PcsI	WCGNNNNNNNCGW	1 cut(s)	81
PctI	GAATGC	1 cut(s)	1150
PdmI	GAANNNNTTC	2 cut(s)	407, 1025
PfeI	GAWTC	7 cut(s)	5, 28, 226, 310, 346, 493, 1561
PflMI	CCANNNNNTGG	1 cut(s)	24
PfoI	TCCNGGA	1 cut(s)	398
PinAI	ACCGGT	1 cut(s)	1044
PleI	GAGTC	2 cut(s)	188, 675
PpsI	GAGTC	2 cut(s)	188, 675
PsiI	TTATAA	2 cut(s)	680, 1536
Psp124BI	GAGCTC	3 cut(s)	249, 264, 1401
Psp6I	CCWGG	5 cut(s)	17, 387, 427, 1220, 1704
PspEI	GGTNACC	1 cut(s)	382
PspGI	CCWGG	5 cut(s)	17, 387, 427, 1220, 1704
PspN4I	GGNNCC	5 cut(s)	101, 276, 489, 640, 746
PspPI	GGNCC	6 cut(s)	341, 416, 488, 629, 639, 958
PstNI	CAGNNNCTG	2 cut(s)	124, 1648
PsuI	RGATCY	1 cut(s)	316
RsaI	GTAC	6 cut(s)	13, 293, 746, 1130, 1429, 1703
RsaNI	GTAC	6 cut(s)	12, 292, 745, 1129, 1428, 1702
RseI	CAYNNNNRTG	1 cut(s)	1167
SacI	GAGCTC	3 cut(s)	249, 264, 1401
SaqAI	TTAA	4 cut(s)	525, 873, 990, 1380
Sau3AI	GATC	9 cut(s)	130, 231, 316, 420, 462, 924, 1203, 1290, 1357
Sau96I	GGNCC	6 cut(s)	341, 416, 488, 629, 639, 958
ScaI	AGTACT	3 cut(s)	293, 1130, 1429
SchI	GAGTC	2 cut(s)	189, 676
ScrFI	CCNGG	9 cut(s)	19, 340, 389, 400, 429, 607, 1222, 1495, 1706
SduI	GDGCHC	5 cut(s)	59, 249, 264, 826, 1401
SexAI	ACCWGGT	1 cut(s)	1704
SfaNI	GCATC	5 cut(s)	210, 1072, 1276, 1303, 1582
Sfr274I	CTCGAG	1 cut(s)	137
SgrAI	CRCCGGYG	1 cut(s)	266
SinI	GGWCC	3 cut(s)	488, 629, 639
SlaI	CTCGAG	1 cut(s)	137
SmiMI	CAYNNNNRTG	1 cut(s)	1167
SmlI	CTYRAG	5 cut(s)	94, 137, 524, 655, 837
SmoI	CTYRAG	5 cut(s)	94, 137, 524, 655, 837
Sse9I	AATT	7 cut(s)	503, 681, 904, 1069, 1470, 1573, 1635
SspMI	CTAG	5 cut(s)	510, 936, 1194, 1317, 1659
SstI	GAGCTC	3 cut(s)	249, 264, 1401
StyD4I	CCNGG	9 cut(s)	17, 338, 387, 398, 427, 605, 1220, 1493, 1704
StyI	CCWWGG	2 cut(s)	279, 1587
TaaI	ACNGT	5 cut(s)	443, 458, 584, 1247, 1745
TaiI	ACGT	1 cut(s)	1458
TaqI	TCGA	9 cut(s)	84, 138, 579, 932, 1136, 1299, 1509, 1582, 1599
TasI	AATT	7 cut(s)	503, 681, 904, 1069, 1470, 1573, 1635
TatI	WGTACW	3 cut(s)	291, 1128, 1427
TauI	GCSGC	6 cut(s)	300, 627, 707, 725, 896, 997
TfiI	GAWTC	7 cut(s)	5, 28, 226, 310, 346, 493, 1561
Tru1I	TTAA	4 cut(s)	525, 873, 990, 1380
Tru9I	TTAA	4 cut(s)	525, 873, 990, 1380
TscAI	CASTG	2 cut(s)	446, 1449
TseFI	GTSAC	1 cut(s)	382
TseI	GCWGC	8 cut(s)	124, 300, 324, 686, 707, 766, 964, 1217
Tsp45I	GTSAC	1 cut(s)	382
TspDTI	ATGAA	2 cut(s)	1055, 1542
TspGWI	ACGGA	2 cut(s)	1179, 1398
TspRI	CASTG	2 cut(s)	446, 1449
Van91I	CCANNNNNTGG	1 cut(s)	24
Vha464I	CTTAAG	1 cut(s)	524
VpaK11BI	GGWCC	3 cut(s)	488, 629, 639
XagI	CCTNNNNNAGG	1 cut(s)	1301
XapI	RAATTY	2 cut(s)	1069, 1635
XhoI	CTCGAG	1 cut(s)	137
XmnI	GAANNNNTTC	2 cut(s)	407, 1025
XspI	CTAG	5 cut(s)	510, 936, 1194, 1317, 1659
ZrmI	AGTACT	3 cut(s)	293, 1130, 1429
Zsp2I	ATGCAT	2 cut(s)	1269, 1720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.