Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012859

Physical Location & Seq

Genomic Coordinates Forward (+)

3192529 .. 3194362

Gene Structure 1834 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1043700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1806 bp

ATGGATTCCCGCATCGCCAGGATTTGGATTCCGGTACTGTTATGGGGCTGTATGAGCACCCATTTCTGCTCTTACGCCGCCGTGGTCGAAGACGACGTGAGGTGCCTTCAGGGGCTGAAGCAGTCGCTGAATGACCCGCTCGGAAAGCTGGCGTCTTGGGATTTCAGGAACCACACCGTCGGATTCATCTGCGATTTCGTTGGCGTCACCTGCTGGAACGATCGGGAGAATCGGATCTTTAGTCTGGAGCTCCGAGACATGGAGATCACCGGCGAGGTCCCGAAGGATATCGAGTACTGCGCCAGCCTCACGAAGCTGGATCTCGGCGGGAACGAGATTTCCGGGCCGATTCCCCCGGATATTTGCGATTGGTTGCCCTTTTTGGTGACCCTCGATTTGTCCGGGAACGACTTCTCCGGCGCTATCCCGCCGGACCTGCAGCACTGTGAGTATCTGAACATTCTGATCCTTTCGGACAACAAGCTTTCGGGTCCGATTCCCTATGAATTTTCTAGCTTGGGTAGGCTTAAGACGTTTTCAGTGGCGAATAATAAGTTGAAGGGGTCTATCCCTTCGTTTTTCGACGGGTTTGATAAGGCGGACTTTGCCGGGAACAGCGGCCTTTGCGGCGGGCCTCTCGGGTCGAAGTGTGGTGGTCTGAGCAAGAAGAGTGTTACTATTATAATTGCTGCCGGAGTTTTTGGCGCTGCGGCGTCTTTGCTGGCTTTTTTGGGGCTGTGGTGGTGGTACCATGTGAGGCAGAGTAAGCGGCGGAGGACGGGAGGTTATGGTGTTGGGAGGGAGGATTGGGCTGAGAGGTTGAGGGCTCATAAGCTTACTCAGGTTTCCTTGTTTCAGAAGCCACTTGTGAAGGTTAAGTTGGCGGATTTGATGGCGGCTACCAATAATTTTAGCCCCAAAAATGTGATCATTTCGACTAGGACAGGGACAACTTACAAGGCTCTGCTGCCTGATGGGTCGGCGCTGGCCATTAAGCGGCTTAGTGCTTGTAAGCTCGGTGAGAAGCAGTTTCGGTTGGAGATGAACCGGTTAGGACAACTTAGACATCCGAATTTGGCCCCCCTTTTGGGGTTCTGCATTGTGGAGGAGGAGAAGCTTTTGGTGTACAAGTACTTGTCGAGTGGGACTTTGCATTCGTTGTTGCACGGAAGTGGTGAGGGATTGGACTGGTCGACTAGGTTTAGGATCGGTTTGGGTGCTGCCAGGGGACTTTCTTGGCTTCACCACGGTTGCCAACCTCCGATTATGCATCAGAACATATGCTCCAATGTGATCCTCCTCGACGAGGATTTTGATGCTAGGATAATGGATTTTGGATTGGCAACGCTCACGGCTTCTGATTCTAAGGAGAGCAGTTTTGTTAACGGAGACTTGGGAGAACTTGGTTATGTAGCTCCAGAGTATCCGAGTACTCTGGTTGCTTCACTGAAAGGGGACGTTTATGGACTTGGAATTGTGCTTCTGGAGCTGGCAACCGGGCAAAAGCCTCTTGAAGTCAGCACTGCTGATGAAGGTTTCAAGGGTAATGTAGTGGATTGGGTGAATCGTCTCTCTAGTTCTGGTCGAACCAAGGATGCCATCGATAAAGCTCTCATCGGAAAAGGGCATGACGAGGAAATTTTGCAGTTTCTGAAGATTGCTAGTAGTTGTGTGATTTCTCGGCCTAAAGACAGGTGGTCTATGTACCAGGTTTACCATGCATTGAAGAGTATGAGCAAAGAACACAGTTTCAGGGGACAAGACGACGAGTTTCCATTGCTTTTTCGCAGGCCAGATAAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002831 GO:0002832 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0008150 GO:0009507 GO:0009536 GO:0016020 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043901 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0065007 GO:0071944 GO:0080134 GO:1900150 GO:1900424 GO:1900425

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

602

Amino Acids

66.38

Weight (kDa)

6.92

Isoelectric Point (pI)

34.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 72	2.4e-09	Leucine rich repeat N-terminal domain
Pkinase	PF00069	315 - 573	7.8e-25	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	327 - 510	2.7e-26	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012859)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G28450
fragaria_vesca	FvH4_6g49690
malus_domestica	MD09G1042400.v1.1 MD17G1043700.v1.1
prunus_persica	Prupe.3G275300_v2.0.a1
pyrus_communis	pycom111g03320
rosa_chinensis	RchiOBHm_Chr2g0169711
rosa_laevigata	RLG00000021896
rosa_multiflora	Rmu_sc0022598.1_g000001 Rmu_ssc0000389.1_g000032 Rmu_ssc0000389.1_g000034
rosa_roxburghii	Rroxscaffold_2G00081850
rosa_rugosa	Rorug02G0542400
rosa_samantha	Rh2AG614800 Rh2BG626900 Rh2CG596400 Rh2DG637800
rosa_wichuraiana	Rw2G050960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	683
AarI	CACCTGC	1 cut(s)	218
Acc36I	ACCTGC	2 cut(s)	218, 444
Acc65I	GGTACC	1 cut(s)	747
AccB1I	GGYRCC	2 cut(s)	102, 747
AccB7I	CCANNNNNTGG	1 cut(s)	24
AccBSI	CCGCTC	1 cut(s)	139
AccI	GTMKAC	1 cut(s)	1193
AclWI	GGATC	5 cut(s)	242, 327, 460, 1214, 1288
AcoI	YGGCCR	1 cut(s)	987
AcsI	RAATTY	3 cut(s)	506, 1072, 1638
AcuI	CTGAAG	3 cut(s)	92, 137, 1673
AcyI	GRCGYC	3 cut(s)	152, 204, 713
AfaI	GTAC	7 cut(s)	36, 296, 749, 1127, 1133, 1432, 1706
AfiI	CCNNNNNNNGG	7 cut(s)	24, 259, 996, 1087, 1088, 1089, 1306
AflII	CTTAAG	1 cut(s)	527
AgeI	ACCGGT	1 cut(s)	1047
AgsI	TTSAA	4 cut(s)	559, 1514, 1540, 1726
AhdI	GACNNNNNGTC	1 cut(s)	1696
AjiI	CACGTC	1 cut(s)	97
AjnI	CCWGG	3 cut(s)	17, 1223, 1707
AjuI	GAANNNNNNNTTGG	2 cut(s)	863, 895
AleI	CACNNNNGTG	1 cut(s)	1170
AloI	GAACNNNNNNTCC	6 cut(s)	323, 355, 398, 430, 1268, 1300
Alw21I	GWGCWC	2 cut(s)	59, 252
Alw26I	GTCTC	3 cut(s)	249, 1383, 1574
AlwI	GGATC	5 cut(s)	242, 327, 460, 1214, 1288
AlwNI	CAGNNNCTG	3 cut(s)	115, 127, 1651
Ama87I	CYCGRG	1 cut(s)	638
AoxI	GGCC	7 cut(s)	344, 619, 632, 987, 1077, 1682, 1790
ApeKI	GCWGC	5 cut(s)	439, 689, 707, 967, 1220
ApoI	RAATTY	3 cut(s)	506, 1072, 1638
ArsI	GACNNNNNNTTYG	6 cut(s)	379, 411, 1138, 1170, 1295, 1327
AsiGI	ACCGGT	1 cut(s)	1047
Asp700I	GAANNNNTTC	1 cut(s)	410
Asp718I	GGTACC	1 cut(s)	747
AspLEI	GCGC	4 cut(s)	302, 422, 707, 985
AspS9I	GGNCC	6 cut(s)	277, 344, 433, 491, 632, 1078
AsuC2I	CCSGG	5 cut(s)	343, 356, 403, 610, 1498
AsuHPI	GGTGA	7 cut(s)	199, 259, 397, 1031, 1187, 1235, 1573
AvaI	CYCGRG	1 cut(s)	638
AvaII	GGWCC	3 cut(s)	277, 433, 491
BalI	TGGCCA	1 cut(s)	989
BanI	GGYRCC	2 cut(s)	102, 747
BanII	GRGCYC	2 cut(s)	252, 829
BbsI	GAAGAC	1 cut(s)	96
Bbv12I	GWGCWC	2 cut(s)	59, 252
BbvI	GCAGC	5 cut(s)	451, 676, 694, 954, 1207
BccI	CCATC	3 cut(s)	886, 968, 1607
BceAI	ACGGC	2 cut(s)	65, 1368
BciT130I	CCWGG	3 cut(s)	19, 1225, 1709
BciVI	GTATCC	1 cut(s)	1434
BclI	TGATCA	1 cut(s)	927
BcnI	CCSGG	5 cut(s)	343, 356, 403, 610, 1498
BcoDI	GTCTC	3 cut(s)	249, 1383, 1574
BfaI	CTAG	6 cut(s)	513, 939, 1197, 1320, 1575, 1662
BfmI	CTRYAG	1 cut(s)	437
BfoI	RGCGCY	3 cut(s)	423, 708, 986
BfrI	CTTAAG	1 cut(s)	527
BfuAI	ACCTGC	2 cut(s)	218, 444
BfuI	GTATCC	1 cut(s)	1434
BglI	GCCNNNNNGGC	1 cut(s)	627
BmcAI	AGTACT	3 cut(s)	296, 1133, 1432
Bme1390I	CCNGG	8 cut(s)	19, 343, 356, 403, 610, 1225, 1498, 1709
Bme18I	GGWCC	3 cut(s)	277, 433, 491
BmeRI	GACNNNNNGTC	1 cut(s)	1696
BmeT110I	CYCGRG	1 cut(s)	638
BmgBI	CACGTC	1 cut(s)	97
BmgT120I	GGNCC	6 cut(s)	277, 344, 433, 491, 632, 1078
BmiI	GGNNCC	6 cut(s)	104, 170, 279, 492, 749, 1080
BmrFI	CCNGG	8 cut(s)	19, 343, 356, 403, 610, 1225, 1498, 1709
BmsI	GCATC	4 cut(s)	21, 1279, 1306, 1585
BpiI	GAAGAC	1 cut(s)	96
BpmI	CTGGAG	3 cut(s)	266, 1401, 1505
BpuMI	CCSGG	5 cut(s)	343, 356, 403, 610, 1498
Bsa29I	ATCGAT	1 cut(s)	1602
BsaHI	GRCGYC	3 cut(s)	152, 204, 713
BsaJI	CCNNGG	5 cut(s)	81, 354, 1224, 1246, 1590
BsaWI	WCCGGW	2 cut(s)	31, 1047
BsaXI	ACNNNNNCTCC	4 cut(s)	398, 428, 1268, 1298
Bsc4I	CCNNNNNNNGG	7 cut(s)	24, 259, 996, 1087, 1088, 1089, 1306
Bse118I	RCCGGY	2 cut(s)	269, 1047
Bse1I	ACTGG	1 cut(s)	1193
Bse3DI	GCAATG	1 cut(s)	1775
BseBI	CCWGG	3 cut(s)	19, 1225, 1709
BseCI	ATCGAT	1 cut(s)	1602
BseDI	CCNNGG	5 cut(s)	81, 354, 1224, 1246, 1590
BseGI	GGATG	2 cut(s)	1066, 1600
BseLI	CCNNNNNNNGG	7 cut(s)	24, 259, 996, 1087, 1088, 1089, 1306
BseMI	GCAATG	1 cut(s)	1775
BseMII	CTCAG	3 cut(s)	650, 804, 854
BseNI	ACTGG	1 cut(s)	1193
BseRI	GAGGAG	3 cut(s)	1121, 1124, 1289
BseXI	GCAGC	5 cut(s)	451, 676, 694, 954, 1207
Bsh1285I	CGRYCG	1 cut(s)	223
BshFI	GGCC	7 cut(s)	346, 621, 634, 989, 1079, 1684, 1792
BshNI	GGYRCC	2 cut(s)	102, 747
BshTI	ACCGGT	1 cut(s)	1047
BshVI	ATCGAT	1 cut(s)	1602
BsiEI	CGRYCG	1 cut(s)	223
BsiHKAI	GWGCWC	2 cut(s)	59, 252
BsiHKCI	CYCGRG	1 cut(s)	638
BslFI	GGGAC	6 cut(s)	263, 961, 1159, 1242, 1469, 1770
BslI	CCNNNNNNNGG	7 cut(s)	24, 259, 996, 1087, 1088, 1089, 1306
BsmAI	GTCTC	3 cut(s)	249, 1383, 1574
BsmBI	CGTCTC	1 cut(s)	1574
BsmFI	GGGAC	6 cut(s)	263, 961, 1159, 1242, 1469, 1770
BsmI	GAATGC	1 cut(s)	1153
BsnI	GGCC	7 cut(s)	346, 621, 634, 989, 1079, 1684, 1792
BsoBI	CYCGRG	1 cut(s)	638
Bsp1286I	GDGCHC	3 cut(s)	59, 252, 829
Bsp1407I	TGTACA	1 cut(s)	1125
Bsp143I	GATC	8 cut(s)	220, 234, 264, 319, 465, 927, 1206, 1293
BspANI	GGCC	7 cut(s)	346, 621, 634, 989, 1079, 1684, 1792
BspCNI	CTCAG	3 cut(s)	651, 805, 853
BspDI	ATCGAT	1 cut(s)	1602
BspLI	GGNNCC	6 cut(s)	104, 170, 279, 492, 749, 1080
BspMAI	CTGCAG	1 cut(s)	441
BspMI	ACCTGC	2 cut(s)	218, 444
BspPI	GGATC	5 cut(s)	242, 327, 460, 1214, 1288
BspT107I	GGYRCC	2 cut(s)	102, 747
BspTI	CTTAAG	1 cut(s)	527
BsrBI	CCGCTC	1 cut(s)	139
BsrDI	GCAATG	1 cut(s)	1775
BsrFI	RCCGGY	2 cut(s)	269, 1047
BsrGI	TGTACA	1 cut(s)	1125
BsrI	ACTGG	1 cut(s)	1193
BssAI	RCCGGY	2 cut(s)	269, 1047
BssECI	CCNNGG	5 cut(s)	81, 354, 1224, 1246, 1590
BssMI	GATC	8 cut(s)	220, 234, 264, 319, 465, 927, 1206, 1293
BssNI	GRCGYC	3 cut(s)	152, 204, 713
BssT1I	CCWWGG	1 cut(s)	1590
Bst2UI	CCWGG	3 cut(s)	19, 1225, 1709
Bst4CI	ACNGT	5 cut(s)	39, 178, 446, 1250, 1748
Bst6I	CTCTTC	2 cut(s)	662, 1721
BstACI	GRCGYC	3 cut(s)	152, 204, 713
BstAFI	CTTAAG	1 cut(s)	527
BstAUI	TGTACA	1 cut(s)	1125
BstC8I	GCNNGC	7 cut(s)	150, 304, 632, 723, 987, 1491, 1790
BstDEI	CTNAG	6 cut(s)	659, 813, 840, 1001, 1061, 1365
BstDSI	CCRYGG	2 cut(s)	81, 1246
BstEII	GGTNACC	1 cut(s)	385
BstENI	CCTNNNNNAGG	1 cut(s)	1304
BstF5I	GGATG	2 cut(s)	1066, 1600
BstH2I	RGCGCY	3 cut(s)	423, 708, 986
BstHHI	GCGC	4 cut(s)	302, 422, 707, 985
BstKTI	GATC	8 cut(s)	223, 237, 267, 322, 468, 930, 1209, 1296
BstMAI	GTCTC	3 cut(s)	249, 1383, 1574
BstMBI	GATC	8 cut(s)	220, 234, 264, 319, 465, 927, 1206, 1293
BstMCI	CGRYCG	1 cut(s)	223
BstMWI	GCNNNNNNNGC	9 cut(s)	54, 145, 210, 436, 605, 624, 627, 766, 1486
BstNI	CCWGG	3 cut(s)	19, 1225, 1709
BstPI	GGTNACC	1 cut(s)	385
BstSCI	CCNGG	8 cut(s)	17, 341, 354, 401, 608, 1223, 1496, 1707
BstSFI	CTRYAG	1 cut(s)	437
BstV1I	GCAGC	5 cut(s)	451, 676, 694, 954, 1207
BstV2I	GAAGAC	1 cut(s)	96
BstX2I	RGATCY	2 cut(s)	234, 319
BstYI	RGATCY	2 cut(s)	234, 319
Bsu15I	ATCGAT	1 cut(s)	1602
BsuI	GTATCC	1 cut(s)	1434
BsuRI	GGCC	7 cut(s)	346, 621, 634, 989, 1079, 1684, 1792
BsuTUI	ATCGAT	1 cut(s)	1602
BtgI	CCRYGG	2 cut(s)	81, 1246
BtrI	CACGTC	1 cut(s)	97
BtsCI	GGATG	2 cut(s)	1066, 1600
BtsI	GCAGTG	1 cut(s)	1521
BtsIMutI	CAGTG	4 cut(s)	442, 546, 1445, 1521
BveI	ACCTGC	2 cut(s)	218, 444
Cac8I	GCNNGC	7 cut(s)	150, 304, 632, 723, 987, 1491, 1790
CaiI	CAGNNNCTG	3 cut(s)	115, 127, 1651
CfoI	GCGC	4 cut(s)	302, 422, 707, 985
Cfr10I	RCCGGY	2 cut(s)	269, 1047
Cfr13I	GGNCC	6 cut(s)	277, 344, 433, 491, 632, 1078
ClaI	ATCGAT	1 cut(s)	1602
CseI	GACGC	3 cut(s)	141, 193, 702
CsiI	ACCWGGT	1 cut(s)	1707
Csp6I	GTAC	7 cut(s)	35, 295, 748, 1126, 1132, 1431, 1705
CspAI	ACCGGT	1 cut(s)	1047
CviAII	CATG	4 cut(s)	259, 752, 1628, 1718
CviQI	GTAC	7 cut(s)	35, 295, 748, 1126, 1132, 1431, 1705
DdeI	CTNAG	6 cut(s)	659, 813, 840, 1001, 1061, 1365
DpnI	GATC	8 cut(s)	222, 236, 266, 321, 467, 929, 1208, 1295
DpnII	GATC	8 cut(s)	220, 234, 264, 319, 465, 927, 1206, 1293
DriI	GACNNNNNGTC	1 cut(s)	1696
EaeI	YGGCCR	1 cut(s)	987
Eam1104I	CTCTTC	2 cut(s)	662, 1721
Eam1105I	GACNNNNNGTC	1 cut(s)	1696
EarI	CTCTTC	2 cut(s)	662, 1721
EciI	GGCGGA	3 cut(s)	614, 787, 899
Ecl136II	GAGCTC	1 cut(s)	250
Eco130I	CCWWGG	1 cut(s)	1590
Eco24I	GRGCYC	2 cut(s)	252, 829
Eco32I	GATATC	1 cut(s)	289
Eco47I	GGWCC	3 cut(s)	277, 433, 491
Eco53kI	GAGCTC	1 cut(s)	250
Eco57I	CTGAAG	3 cut(s)	92, 137, 1673
Eco88I	CYCGRG	1 cut(s)	638
Eco91I	GGTNACC	1 cut(s)	385
EcoICRI	GAGCTC	1 cut(s)	250
EcoNI	CCTNNNNNAGG	1 cut(s)	1304
EcoO109I	RGGNCCY	1 cut(s)	277
EcoO65I	GGTNACC	1 cut(s)	385
EcoRII	CCWGG	3 cut(s)	17, 1223, 1707
EcoRV	GATATC	1 cut(s)	289
EcoT14I	CCWWGG	1 cut(s)	1590
EcoT22I	ATGCAT	2 cut(s)	1272, 1723
EcoT38I	GRGCYC	2 cut(s)	252, 829
ErhI	CCWWGG	1 cut(s)	1590
Esp3I	CGTCTC	1 cut(s)	1574
FaeI	CATG	4 cut(s)	262, 755, 1631, 1721
FalI	AAGNNNNNCTT	2 cut(s)	587, 619
FaqI	GGGAC	6 cut(s)	263, 961, 1159, 1242, 1469, 1770
FatI	CATG	4 cut(s)	258, 751, 1627, 1717
FauI	CCCGC	5 cut(s)	17, 144, 320, 435, 623
FauNDI	CATATG	1 cut(s)	1280
FbaI	TGATCA	1 cut(s)	927
FblI	GTMKAC	1 cut(s)	1193
FokI	GGATG	2 cut(s)	1053, 1607
FriOI	GRGCYC	2 cut(s)	252, 829
FspBI	CTAG	6 cut(s)	513, 939, 1197, 1320, 1575, 1662
GlaI	GCGC	4 cut(s)	301, 421, 706, 984
GsuI	CTGGAG	3 cut(s)	266, 1401, 1505
HaeII	RGCGCY	3 cut(s)	423, 708, 986
HaeIII	GGCC	7 cut(s)	346, 621, 634, 989, 1079, 1684, 1792
HgaI	GACGC	3 cut(s)	141, 193, 702
HhaI	GCGC	4 cut(s)	302, 422, 707, 985
Hin1I	GRCGYC	3 cut(s)	152, 204, 713
Hin1II	CATG	4 cut(s)	262, 755, 1631, 1721
Hin6I	GCGC	4 cut(s)	300, 420, 705, 983
HinP1I	GCGC	4 cut(s)	300, 420, 705, 983
HincII	GTYRAC	2 cut(s)	1194, 1384
HindII	GTYRAC	2 cut(s)	1194, 1384
HindIII	AAGCTT	3 cut(s)	482, 833, 1115
HinfI	GANTC	8 cut(s)	5, 28, 183, 229, 349, 496, 1361, 1564
HpaI	GTTAAC	1 cut(s)	1384
HphI	GGTGA	7 cut(s)	199, 259, 397, 1031, 1187, 1235, 1573
Hpy166II	GTNNAC	4 cut(s)	1126, 1194, 1384, 1714
Hpy188III	TCNNGA	8 cut(s)	166, 224, 245, 280, 310, 1418, 1484, 1511
Hpy8I	GTNNAC	4 cut(s)	1126, 1194, 1384, 1714
Hpy99I	CGWCG	5 cut(s)	98, 182, 587, 1307, 1769
HpyAV	CCTTC	6 cut(s)	116, 277, 553, 582, 865, 1526
HpyCH4III	ACNGT	5 cut(s)	39, 178, 446, 1250, 1748
HpyCH4IV	ACGT	3 cut(s)	96, 533, 1458
HpyCH4V	TGCA	7 cut(s)	439, 1098, 1153, 1165, 1270, 1645, 1721
HpyF10VI	GCNNNNNNNGC	9 cut(s)	54, 145, 210, 436, 605, 624, 627, 766, 1486
HpyF3I	CTNAG	6 cut(s)	659, 813, 840, 1001, 1061, 1365
HpySE526I	ACGT	3 cut(s)	96, 533, 1458
Hsp92I	GRCGYC	3 cut(s)	152, 204, 713
Hsp92II	CATG	4 cut(s)	262, 755, 1631, 1721
HspAI	GCGC	4 cut(s)	300, 420, 705, 983
KpnI	GGTACC	1 cut(s)	751
Ksp22I	TGATCA	1 cut(s)	927
KspAI	GTTAAC	1 cut(s)	1384
Kzo9I	GATC	8 cut(s)	220, 234, 264, 319, 465, 927, 1206, 1293
LmnI	GCTCC	5 cut(s)	247, 255, 1289, 1420, 1486
Lsp1109I	GCAGC	5 cut(s)	451, 676, 694, 954, 1207
LweI	GCATC	4 cut(s)	21, 1279, 1306, 1585
MabI	ACCWGGT	1 cut(s)	1707
MaeI	CTAG	6 cut(s)	513, 939, 1197, 1320, 1575, 1662
MaeII	ACGT	3 cut(s)	96, 533, 1458
MaeIII	GTNAC	3 cut(s)	205, 385, 673
MalI	GATC	8 cut(s)	222, 236, 266, 321, 467, 929, 1208, 1295
MbiI	CCGCTC	1 cut(s)	139
MboI	GATC	8 cut(s)	220, 234, 264, 319, 465, 927, 1206, 1293
MboII	GAAGA	4 cut(s)	101, 679, 1666, 1738
MflI	RGATCY	2 cut(s)	234, 319
MhlI	GDGCHC	3 cut(s)	59, 252, 829
MlsI	TGGCCA	1 cut(s)	989
MluCI	AATT	6 cut(s)	506, 684, 907, 1072, 1473, 1638
MluNI	TGGCCA	1 cut(s)	989
MmeI	TCCRAC	2 cut(s)	160, 1017
Mox20I	TGGCCA	1 cut(s)	989
Mph1103I	ATGCAT	2 cut(s)	1272, 1723
MroXI	GAANNNNTTC	1 cut(s)	410
MscI	TGGCCA	1 cut(s)	989
MseI	TTAA	4 cut(s)	528, 876, 993, 1383
MslI	CAYNNNNRTG	1 cut(s)	1170
Msp20I	TGGCCA	1 cut(s)	989
MspA1I	CMGCKG	1 cut(s)	618
MspCI	CTTAAG	1 cut(s)	527
MspR9I	CCNGG	8 cut(s)	19, 343, 356, 403, 610, 1225, 1498, 1709
Mva1269I	GAATGC	1 cut(s)	1153
MvaI	CCWGG	3 cut(s)	19, 1225, 1709
MwoI	GCNNNNNNNGC	9 cut(s)	54, 145, 210, 436, 605, 624, 627, 766, 1486
NciI	CCSGG	5 cut(s)	343, 356, 403, 610, 1498
NdeI	CATATG	1 cut(s)	1280
NdeII	GATC	8 cut(s)	220, 234, 264, 319, 465, 927, 1206, 1293
NlaIII	CATG	4 cut(s)	262, 755, 1631, 1721
NlaIV	GGNNCC	6 cut(s)	104, 170, 279, 492, 749, 1080
NmeAIII	GCCGAG	2 cut(s)	303, 1660
NmuCI	GTSAC	2 cut(s)	205, 385
NsiI	ATGCAT	2 cut(s)	1272, 1723
OliI	CACNNNNGTG	1 cut(s)	1170
PaqCI	CACCTGC	1 cut(s)	218
PcsI	WCGNNNNNNNCGW	2 cut(s)	93, 330
PctI	GAATGC	1 cut(s)	1153
PdmI	GAANNNNTTC	1 cut(s)	410
PfeI	GAWTC	8 cut(s)	5, 28, 183, 229, 349, 496, 1361, 1564
PflMI	CCANNNNNTGG	1 cut(s)	24
PfoI	TCCNGGA	1 cut(s)	401
PinAI	ACCGGT	1 cut(s)	1047
Ple19I	CGATCG	1 cut(s)	223
PpuMI	RGGWCCY	1 cut(s)	277
PsiI	TTATAA	1 cut(s)	683
Psp124BI	GAGCTC	1 cut(s)	252
Psp5II	RGGWCCY	1 cut(s)	277
Psp6I	CCWGG	3 cut(s)	17, 1223, 1707
PspEI	GGTNACC	1 cut(s)	385
PspGI	CCWGG	3 cut(s)	17, 1223, 1707
PspN4I	GGNNCC	6 cut(s)	104, 170, 279, 492, 749, 1080
PspPI	GGNCC	6 cut(s)	277, 344, 433, 491, 632, 1078
PspPPI	RGGWCCY	1 cut(s)	277
PstI	CTGCAG	1 cut(s)	441
PstNI	CAGNNNCTG	3 cut(s)	115, 127, 1651
PsuI	RGATCY	2 cut(s)	234, 319
PvuI	CGATCG	1 cut(s)	223
RsaI	GTAC	7 cut(s)	36, 296, 749, 1127, 1133, 1432, 1706
RsaNI	GTAC	7 cut(s)	35, 295, 748, 1126, 1132, 1431, 1705
RseI	CAYNNNNRTG	1 cut(s)	1170
SacI	GAGCTC	1 cut(s)	252
SalI	GTCGAC	1 cut(s)	1192
SaqAI	TTAA	4 cut(s)	528, 876, 993, 1383
Sau3AI	GATC	8 cut(s)	220, 234, 264, 319, 465, 927, 1206, 1293
Sau96I	GGNCC	6 cut(s)	277, 344, 433, 491, 632, 1078
ScaI	AGTACT	3 cut(s)	296, 1133, 1432
ScrFI	CCNGG	8 cut(s)	19, 343, 356, 403, 610, 1225, 1498, 1709
SduI	GDGCHC	3 cut(s)	59, 252, 829
SexAI	ACCWGGT	1 cut(s)	1707
SfaNI	GCATC	4 cut(s)	21, 1279, 1306, 1585
SfcI	CTRYAG	1 cut(s)	437
SgrAI	CRCCGGYG	1 cut(s)	269
SinI	GGWCC	3 cut(s)	277, 433, 491
SmiMI	CAYNNNNRTG	1 cut(s)	1170
SmlI	CTYRAG	1 cut(s)	527
SmoI	CTYRAG	1 cut(s)	527
Sse9I	AATT	6 cut(s)	506, 684, 907, 1072, 1473, 1638
SspMI	CTAG	6 cut(s)	513, 939, 1197, 1320, 1575, 1662
SstI	GAGCTC	1 cut(s)	252
StyD4I	CCNGG	8 cut(s)	17, 341, 354, 401, 608, 1223, 1496, 1707
StyI	CCWWGG	1 cut(s)	1590
TaaI	ACNGT	5 cut(s)	39, 178, 446, 1250, 1748
TaiI	ACGT	3 cut(s)	99, 536, 1461
TasI	AATT	6 cut(s)	506, 684, 907, 1072, 1473, 1638
TatI	WGTACW	4 cut(s)	294, 1125, 1131, 1430
TauI	GCSGC	7 cut(s)	80, 621, 630, 713, 772, 899, 1000
TfiI	GAWTC	8 cut(s)	5, 28, 183, 229, 349, 496, 1361, 1564
Tru1I	TTAA	4 cut(s)	528, 876, 993, 1383
Tru9I	TTAA	4 cut(s)	528, 876, 993, 1383
TscAI	CASTG	4 cut(s)	449, 546, 1452, 1528
TseFI	GTSAC	2 cut(s)	205, 385
TseI	GCWGC	5 cut(s)	439, 689, 707, 967, 1220
Tsp45I	GTSAC	2 cut(s)	205, 385
TspDTI	ATGAA	4 cut(s)	175, 519, 1058, 1545
TspGWI	ACGGA	2 cut(s)	1182, 1401
TspRI	CASTG	4 cut(s)	449, 546, 1452, 1528
Van91I	CCANNNNNTGG	1 cut(s)	24
Vha464I	CTTAAG	1 cut(s)	527
VpaK11BI	GGWCC	3 cut(s)	277, 433, 491
XagI	CCTNNNNNAGG	1 cut(s)	1304
XapI	RAATTY	3 cut(s)	506, 1072, 1638
XmiI	GTMKAC	1 cut(s)	1193
XmnI	GAANNNNTTC	1 cut(s)	410
XspI	CTAG	6 cut(s)	513, 939, 1197, 1320, 1575, 1662
ZrmI	AGTACT	3 cut(s)	296, 1133, 1432
Zsp2I	ATGCAT	2 cut(s)	1272, 1723

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.