AT3G52300

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a ATP6 static relative to the rotary elements

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016099
Physical Location & Seq
Forward (+)
19396424 .. 19398444
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G52300.2

Sequence Viewer

Length: 507 bp
ATGAGCGGAGCCGGTAAGAAAATCGCGGATGTGGCTTTCAAAGCTTCAAGGACTATCGATTGGGATGGTATGGCTAAGGTCCTTGTCACAGATGAGGCTCGTAGAGAGTTCTCTAACCTTCGTCGTGCTTTCGATGAGGTTAACACACAGCTCCAGACCAAATTTAGTCAGGAACCTGAACCTATAGATTGGGATTACTATAGGAAGGGTATTGGAGCTGGCATTGTTGACAAGTACAAGGAAGCTTATGACAGCATTGAGATTCCAAAGTACGTTGACAAAGTTACTCCTGAATACAAGCCAAAGTTTGATGCTTTGTTGGTGGAACTGAAAGAAGCAGAACAGAAATCGCTCAAGGAGTCTGAACGGTTGGAGAAAGAAATTGCTGATGTCCAAGAGATCAGCAAAAAGCTCAGCACCATGACTGCAGATGAGTACTTTGAGAAGCACCCGGAACTCAAAAAGAAGTTTGATGACGAAATCCGTAATGACAACTGGGGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000274 GO:0000276 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005753 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009651 GO:0009987 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016469 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019866 GO:0022626 GO:0031090 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031981 GO:0031984 GO:0032991 GO:0033177 GO:0034220 GO:0034357 GO:0034641 GO:0034654 GO:0042651 GO:0042788 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044436 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044455 GO:0044464 GO:0045259 GO:0045263 GO:0045265 GO:0046034 GO:0046390 GO:0046483 GO:0046872 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0055086 GO:0070013 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0098798 GO:0098800 GO:0098805 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko05010 ko05012 ko05016 map00190 map01100 map04714 map05010 map05012 map05016
Pfam Domains
Protein Families
Mt_ATP-synt_D

Protein Analysis

168

Amino Acids

19.59

Weight (kDa)

5.09

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mt_ATP-synt_D PF05873 15 - 98 2.5e-12 ATP synthase D chain, mitochondrial (ATP5H)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52300
fragaria_vesca FvH4_7g20110
malus_domestica MD01G1130700.v1.1 MD07G1178800.v1.1
prunus_persica Prupe.2G217900_v2.0.a1
pyrus_communis pycom01g13950 pycom07g17250
rosa_chinensis RchiOBHm_Chr1g0364071
rosa_laevigata RLG00000027519
rosa_multiflora Rmu_sc0000071.1_g000026
rosa_roxburghii Rroxscaffold_4G00292670
rosa_rugosa Rorug01G0313300
rosa_samantha Rh1AG320700 Rh1CG300300
rosa_wichuraiana Rw1G028430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 1 cut(s) 26
AciI CCGC 2 cut(s) 6, 26
AcsI RAATTY 1 cut(s) 161
AfaI GTAC 3 cut(s) 236, 272, 437
AgsI TTSAA 2 cut(s) 40, 48
AluBI AGCT 5 cut(s) 44, 151, 218, 245, 412
AluI AGCT 5 cut(s) 44, 151, 218, 245, 412
ApoI RAATTY 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 79
AsuC2I CCSGG 1 cut(s) 452
AvaII GGWCC 1 cut(s) 79
BccI CCATC 1 cut(s) 59
BciVI GTATCC 1 cut(s) 494
BcnI CCSGG 1 cut(s) 452
BfmI CTRYAG 3 cut(s) 183, 199, 426
BfuI GTATCC 1 cut(s) 494
BlpI GCTNAGC 1 cut(s) 413
BmcAI AGTACT 1 cut(s) 437
Bme1390I CCNGG 1 cut(s) 452
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 2 cut(s) 10, 174
BmrFI CCNGG 1 cut(s) 452
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 1 cut(s) 301
BmuI ACTGGG 1 cut(s) 505
BpmI CTGGAG 1 cut(s) 137
Bpu10I CCTNAGC 1 cut(s) 75
Bpu1102I GCTNAGC 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 452
Bsa29I ATCGAT 1 cut(s) 57
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 1 cut(s) 500
BseCI ATCGAT 1 cut(s) 57
BseGI GGATG 2 cut(s) 34, 70
BseMII CTCAG 1 cut(s) 427
BseNI ACTGG 1 cut(s) 500
Bsh1236I CGCG 1 cut(s) 26
BshVI ATCGAT 1 cut(s) 57
BsiSI CCGG 2 cut(s) 12, 452
Bsp143I GATC 1 cut(s) 399
Bsp1720I GCTNAGC 1 cut(s) 413
BspACI CCGC 2 cut(s) 6, 26
BspCNI CTCAG 1 cut(s) 426
BspDI ATCGAT 1 cut(s) 57
BspFNI CGCG 1 cut(s) 26
BspLI GGNNCC 2 cut(s) 10, 174
BspMAI CTGCAG 1 cut(s) 430
BsrBI CCGCTC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 1 cut(s) 500
BssAI RCCGGY 1 cut(s) 11
BssMI GATC 1 cut(s) 399
Bst4CI ACNGT 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 2 cut(s) 75, 413
BstF5I GGATG 2 cut(s) 34, 70
BstFNI CGCG 1 cut(s) 26
BstKTI GATC 1 cut(s) 402
BstMBI GATC 1 cut(s) 399
BstMWI GCNNNNNNNGC 2 cut(s) 32, 41
BstSCI CCNGG 1 cut(s) 450
BstSFI CTRYAG 3 cut(s) 183, 199, 426
BstUI CGCG 1 cut(s) 26
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 1 cut(s) 494
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 2 cut(s) 34, 70
Cac8I GCNNGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 79
ClaI ATCGAT 1 cut(s) 57
Csp6I GTAC 3 cut(s) 235, 271, 436
CviAII CATG 1 cut(s) 421
CviQI GTAC 3 cut(s) 235, 271, 436
DdeI CTNAG 2 cut(s) 75, 413
DpnI GATC 1 cut(s) 401
DpnII GATC 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 79
EcoO109I RGGNCCY 1 cut(s) 79
FaeI CATG 1 cut(s) 424
FaiI YATR 5 cut(s) 71, 185, 201, 249, 422
FatI CATG 1 cut(s) 420
FokI GGATG 2 cut(s) 41, 77
GsuI CTGGAG 1 cut(s) 137
HapII CCGG 2 cut(s) 12, 452
Hin1II CATG 1 cut(s) 424
HincII GTYRAC 3 cut(s) 142, 229, 277
HindII GTYRAC 3 cut(s) 142, 229, 277
HindIII AAGCTT 2 cut(s) 42, 243
HinfI GANTC 2 cut(s) 262, 359
HpaI GTTAAC 1 cut(s) 142
HpaII CCGG 2 cut(s) 12, 452
Hpy166II GTNNAC 3 cut(s) 142, 229, 277
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 3 cut(s) 154, 170, 290
Hpy8I GTNNAC 3 cut(s) 142, 229, 277
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 2 cut(s) 128, 199
HpyCH4III ACNGT 1 cut(s) 369
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 1 cut(s) 428
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 41
HpyF3I CTNAG 2 cut(s) 75, 413
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 1 cut(s) 424
KspAI GTTAAC 1 cut(s) 142
Kzo9I GATC 1 cut(s) 399
LmnI GCTCC 3 cut(s) 8, 156, 215
LpnPI CCDG 8 cut(s) 25, 155, 167, 189, 204, 303, 465, 481
LweI GCATC 1 cut(s) 301
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 2 cut(s) 85, 283
MalI GATC 1 cut(s) 401
MbiI CCGCTC 1 cut(s) 6
MboI GATC 1 cut(s) 399
MluCI AATT 2 cut(s) 161, 381
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 2 cut(s) 88, 130
MseI TTAA 1 cut(s) 141
MspI CCGG 2 cut(s) 12, 452
MspR9I CCNGG 1 cut(s) 452
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 32, 41
NciI CCSGG 1 cut(s) 452
NdeII GATC 1 cut(s) 399
NlaIII CATG 1 cut(s) 424
NlaIV GGNNCC 2 cut(s) 10, 174
NmuCI GTSAC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 262
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PpuMI RGGWCCY 1 cut(s) 79
Psp5II RGGWCCY 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 10, 174
PspPI GGNCC 1 cut(s) 79
PspPPI RGGWCCY 1 cut(s) 79
PstI CTGCAG 1 cut(s) 430
RsaI GTAC 3 cut(s) 236, 272, 437
RsaNI GTAC 3 cut(s) 235, 271, 436
SaqAI TTAA 1 cut(s) 141
Sau3AI GATC 1 cut(s) 399
Sau96I GGNCC 1 cut(s) 79
ScaI AGTACT 1 cut(s) 437
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 1 cut(s) 452
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 3 cut(s) 183, 199, 426
SinI GGWCC 1 cut(s) 79
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 2 cut(s) 161, 381
SsiI CCGC 2 cut(s) 6, 26
StyD4I CCNGG 1 cut(s) 450
TaaI ACNGT 1 cut(s) 369
TaiI ACGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 57, 132
TasI AATT 2 cut(s) 161, 381
TatI WGTACW 2 cut(s) 234, 435
TfiI GAWTC 1 cut(s) 262
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TseFI GTSAC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 85
TspGWI ACGGA 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 1 cut(s) 161
ZrmI AGTACT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.