MD01G1130700.v1.1

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a ATP6 static relative to the rotary elements

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016099
Physical Location & Seq
Reverse (-)
24126265 .. 24128619
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1130700.v1.1.491

Sequence Viewer

Length: 507 bp
ATGAGCGGCGCCGGGAAGAAAGTGGCCGATGTGGCTTTCAAGGCCTCGAGGAACATCGACTGGGAGGGCATGGCCAAGCTCCTCGTCTCCGATGAGGCTCGCAAAGAGTTCGCCTCTCTTCGTCGCGCCTTCGACGAGGTCAACACTACCCTCCAGACCAAGTTCAGCCAGGAGCCTGAACCCATAGACTGGGAGTACTACAGGAAAGGAATCGGGTCTCGCTTGGTGGATATGTACAAAGAGGCTTATGACAGCGTTGAAATCCCCAAGTATGTAGACACAGTCACTCCTCAGTACAAGCCTAAATTTGATCAATTGTTGGTGGAACTAAAAGAAGCAGAGGAAAAGTCTTTGAAGGAGTCTGAGCGTTTGGAGAAGGAGATAGTTGAAGTGCAAGAGTTGAAGAAAAAGATAAGCACCATGACTGCAGATGAGTACTTTGAGAAGCATCCAGAGCTGAAGAAGAAATTCGACGATGAAATCCGAAATGACTACTGGGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000274 GO:0000276 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005753 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009651 GO:0009987 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016469 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019866 GO:0022626 GO:0031090 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031981 GO:0031984 GO:0032991 GO:0033177 GO:0034220 GO:0034357 GO:0034641 GO:0034654 GO:0042651 GO:0042788 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044436 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044455 GO:0044464 GO:0045259 GO:0045263 GO:0045265 GO:0046034 GO:0046390 GO:0046483 GO:0046872 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0055086 GO:0070013 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0098798 GO:0098800 GO:0098805 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko05010 ko05012 ko05016 map00190 map01100 map04714 map05010 map05012 map05016
Pfam Domains
Protein Families
Mt_ATP-synt_D

Protein Analysis

169

Amino Acids

19.78

Weight (kDa)

5.12

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mt_ATP-synt_D PF05873 15 - 166 7.1e-19 ATP synthase D chain, mitochondrial (ATP5H)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52300
fragaria_vesca FvH4_7g20110
malus_domestica MD01G1130700.v1.1 MD07G1178800.v1.1
prunus_persica Prupe.2G217900_v2.0.a1
pyrus_communis pycom01g13950 pycom07g17250
rosa_chinensis RchiOBHm_Chr1g0364071
rosa_laevigata RLG00000027519
rosa_multiflora Rmu_sc0000071.1_g000026
rosa_roxburghii Rroxscaffold_4G00292670
rosa_rugosa Rorug01G0313300
rosa_samantha Rh1AG320700 Rh1CG300300
rosa_wichuraiana Rw1G028430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 8
AccB7I CCANNNNNTGG 1 cut(s) 189
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 126
AciI CCGC 1 cut(s) 6
AcoI YGGCCR 2 cut(s) 24, 72
AcsI RAATTY 2 cut(s) 305, 467
AcuI CTGAAG 1 cut(s) 479
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 4 cut(s) 197, 236, 296, 437
AfiI CCNNNNNNNGG 1 cut(s) 189
AgsI TTSAA 5 cut(s) 40, 260, 355, 389, 403
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 2 cut(s) 79, 457
AluI AGCT 2 cut(s) 79, 457
Alw26I GTCTC 2 cut(s) 91, 222
Ama87I CYCGRG 1 cut(s) 46
AoxI GGCC 3 cut(s) 24, 42, 72
ApoI RAATTY 2 cut(s) 305, 467
Asp700I GAANNNNTTC 1 cut(s) 467
AspLEI GCGC 2 cut(s) 11, 128
AsuC2I CCSGG 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 46
BalI TGGCCA 1 cut(s) 74
BanI GGYRCC 1 cut(s) 8
BcgI CGANNNNNNTGC 2 cut(s) 91, 125
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 310
BcnI CCSGG 1 cut(s) 13
BcoDI GTCTC 2 cut(s) 91, 222
BfmI CTRYAG 2 cut(s) 199, 426
BfoI RGCGCY 1 cut(s) 12
BglI GCCNNNNNGGC 1 cut(s) 32
BisI GCNGC 1 cut(s) 7
BlsI GCNGC 1 cut(s) 8
BmcAI AGTACT 2 cut(s) 197, 437
Bme1390I CCNGG 2 cut(s) 13, 170
BmeT110I CYCGRG 1 cut(s) 46
BmiI GGNNCC 2 cut(s) 10, 174
BmrFI CCNGG 2 cut(s) 13, 170
BmrI ACTGGG 3 cut(s) 70, 199, 505
BmsI GCATC 1 cut(s) 457
BmuI ACTGGG 3 cut(s) 70, 199, 505
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpmI CTGGAG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 13
BsaHI GRCGYC 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 222
BsaXI ACNNNNNCTCC 2 cut(s) 271, 301
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse1I ACTGG 3 cut(s) 65, 194, 500
BseBI CCWGG 1 cut(s) 170
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMII CTCAG 2 cut(s) 305, 354
BseNI ACTGG 3 cut(s) 65, 194, 500
BseRI GAGGAG 2 cut(s) 71, 279
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 26, 44, 74
BshNI GGYRCC 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 2 cut(s) 91, 222
BsmBI CGTCTC 1 cut(s) 91
BsnI GGCC 3 cut(s) 26, 44, 74
Bso31I GGTCTC 1 cut(s) 222
BsoBI CYCGRG 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 1 cut(s) 310
BspACI CCGC 1 cut(s) 6
BspANI GGCC 3 cut(s) 26, 44, 74
BspCNI CTCAG 2 cut(s) 304, 355
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 2 cut(s) 10, 174
BspMAI CTGCAG 1 cut(s) 430
BspT107I GGYRCC 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 222
BsrBI CCGCTC 1 cut(s) 6
BsrGI TGTACA 1 cut(s) 234
BsrI ACTGG 3 cut(s) 65, 194, 500
BssMI GATC 1 cut(s) 310
BssNI GRCGYC 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 9
BstAUI TGTACA 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 2 cut(s) 291, 363
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 1 cut(s) 126
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 128
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 2 cut(s) 91, 222
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 32, 41, 454
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 2 cut(s) 11, 168
BstSFI CTRYAG 2 cut(s) 199, 426
BstUI CGCG 1 cut(s) 126
BsuRI GGCC 3 cut(s) 26, 44, 74
BtsCI GGATG 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 100
CfoI GCGC 2 cut(s) 11, 128
Csp6I GTAC 4 cut(s) 196, 235, 295, 436
CviAII CATG 2 cut(s) 70, 421
CviQI GTAC 4 cut(s) 196, 235, 295, 436
DdeI CTNAG 2 cut(s) 291, 363
DinI GGCGCC 1 cut(s) 10
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
EaeI YGGCCR 2 cut(s) 24, 72
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco147I AGGCCT 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 479
Eco88I CYCGRG 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 168
EgeI GGCGCC 1 cut(s) 10
EheI GGCGCC 1 cut(s) 10
Esp3I CGTCTC 1 cut(s) 91
FaeI CATG 2 cut(s) 73, 424
FaiI YATR 6 cut(s) 71, 185, 233, 249, 273, 422
FatI CATG 2 cut(s) 69, 420
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 7
FokI GGATG 1 cut(s) 435
Fsp4HI GCNGC 1 cut(s) 7
GlaI GCGC 2 cut(s) 10, 127
GluI GCNGC 1 cut(s) 7
GsuI CTGGAG 1 cut(s) 137
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 3 cut(s) 26, 44, 74
HapII CCGG 1 cut(s) 12
HhaI GCGC 2 cut(s) 11, 128
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 2 cut(s) 73, 424
Hin6I GCGC 2 cut(s) 9, 126
HinP1I GCGC 2 cut(s) 9, 126
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 2 cut(s) 210, 359
HpaII CCGG 1 cut(s) 12
Hpy166II GTNNAC 2 cut(s) 142, 277
Hpy188I TCNGA 3 cut(s) 91, 364, 485
Hpy188III TCNNGA 2 cut(s) 154, 452
Hpy8I GTNNAC 2 cut(s) 142, 277
Hpy99I CGWCG 3 cut(s) 126, 137, 476
HpyAV CCTTC 3 cut(s) 139, 349, 370
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4V TGCA 2 cut(s) 394, 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 41, 454
HpyF3I CTNAG 2 cut(s) 291, 363
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 2 cut(s) 73, 424
HspAI GCGC 2 cut(s) 9, 126
KasI GGCGCC 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 1 cut(s) 310
LmnI GCTCC 2 cut(s) 84, 172
LweI GCATC 1 cut(s) 457
MaeIII GTNAC 1 cut(s) 283
MalI GATC 1 cut(s) 312
MbiI CCGCTC 1 cut(s) 6
MboI GATC 1 cut(s) 310
MboII GAAGA 5 cut(s) 28, 110, 415, 472, 475
MfeI CAATTG 1 cut(s) 314
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 3 cut(s) 305, 314, 467
MluNI TGGCCA 1 cut(s) 74
Mly113I GGCGCC 1 cut(s) 9
MlyI GAGTC 1 cut(s) 368
Mox20I TGGCCA 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 74
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 2 cut(s) 13, 170
MunI CAATTG 1 cut(s) 314
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 3 cut(s) 32, 41, 454
NarI GGCGCC 1 cut(s) 9
NciI CCSGG 1 cut(s) 13
NdeII GATC 1 cut(s) 310
NlaIII CATG 2 cut(s) 73, 424
NlaIV GGNNCC 2 cut(s) 10, 174
NmuCI GTSAC 1 cut(s) 283
PaeR7I CTCGAG 1 cut(s) 46
PceI AGGCCT 1 cut(s) 44
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 1 cut(s) 210
PflFI GACNNNGTC 2 cut(s) 137, 281
PflMI CCANNNNNTGG 1 cut(s) 189
PkrI GCNGC 1 cut(s) 8
PleI GAGTC 1 cut(s) 367
PluTI GGCGCC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 367
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 10, 174
PspXI VCTCGAGB 1 cut(s) 46
PstI CTGCAG 1 cut(s) 430
PsyI GACNNNGTC 2 cut(s) 137, 281
RsaI GTAC 4 cut(s) 197, 236, 296, 437
RsaNI GTAC 4 cut(s) 196, 235, 295, 436
SatI GCNGC 1 cut(s) 7
Sau3AI GATC 1 cut(s) 310
ScaI AGTACT 2 cut(s) 197, 437
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 13, 170
SetI ASST 3 cut(s) 81, 141, 459
SfaNI GCATC 1 cut(s) 457
SfcI CTRYAG 2 cut(s) 199, 426
SfoI GGCGCC 1 cut(s) 10
Sfr274I CTCGAG 1 cut(s) 46
SlaI CTCGAG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 3 cut(s) 305, 314, 467
SseBI AGGCCT 1 cut(s) 44
SsiI CCGC 1 cut(s) 6
SspDI GGCGCC 1 cut(s) 8
StuI AGGCCT 1 cut(s) 44
StyD4I CCNGG 2 cut(s) 11, 168
TaaI ACNGT 1 cut(s) 283
TaqI TCGA 4 cut(s) 47, 57, 132, 471
TasI AATT 3 cut(s) 305, 314, 467
TatI WGTACW 4 cut(s) 195, 234, 294, 435
TauI GCSGC 1 cut(s) 9
TfiI GAWTC 1 cut(s) 210
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 492
Tth111I GACNNNGTC 2 cut(s) 137, 281
Van91I CCANNNNNTGG 1 cut(s) 189
XapI RAATTY 2 cut(s) 305, 467
XhoI CTCGAG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 467
ZrmI AGTACT 2 cut(s) 197, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.