pycom07g17250

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a ATP6 static relative to the rotary elements

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0016099
Physical Location & Seq
Reverse (-)
19767839 .. 19770739
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g17250.4

Sequence Viewer

Length: 612 bp
ATGTTAGATGTAACGCGTTTCCCTTCGGCCGAAGCAGCAGCAGCAACAGGAACAGCAACAAAAGGGGCATCCGATCATCTGATTCATATTTGTTTGGCCAGCGAGAAAGCGAGAGAGAAAATGAGCGGCGCCGGGAAGAAAGTGGCCGATGTGGCTTTCAAGGCCTCGAGGAACATCGACTGGGAGGGCATGGCCAAGCTCCTCGTCTCCGATGAGGCTCGCAAAGAGTTCGCCTCTCTTCGTCGCGCCTTCGACGAGGTCAACACTACCCTCCAGACCAAGTTCAGCCAGGAGCCTGAACCCATAGACTGGGAGTACTACAGGAAAGGAATTGGGTCTCGGTTGGTGGATATGTACAAGGAGGCTTATGACAGCGTTGAAATCCCAAAGTATGTAGACACAGTCACACCTCAGTACAAGCCGAAGTTTGATCAATTGGAAGCCGAGGAGAAATCTTTAAAGGAGTCGGAGCGTTTGGAGAAAGAGATAGTTGAAGTGCAAGAGTTGAAGAAAAAGATAAGCACCATGACCGCAGATGAGTACTTTGAGAAGCATCCCGAGCTGAAGAAGAAATTCGATGATGAAATCCGAAATGACTACTGGGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000274 GO:0000276 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005753 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0008270 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009651 GO:0009987 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016469 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019866 GO:0022626 GO:0031090 GO:0031966 GO:0031967 GO:0031974 GO:0031975 GO:0031976 GO:0031981 GO:0031984 GO:0032991 GO:0033177 GO:0034220 GO:0034357 GO:0034641 GO:0034654 GO:0042651 GO:0042788 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044434 GO:0044435 GO:0044436 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044455 GO:0044464 GO:0045259 GO:0045263 GO:0045265 GO:0046034 GO:0046390 GO:0046483 GO:0046872 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0055086 GO:0070013 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0098798 GO:0098800 GO:0098805 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko05010 ko05012 ko05016 map00190 map01100 map04714 map05010 map05012 map05016
Pfam Domains
Protein Families
Mt_ATP-synt_D

Protein Analysis

204

Amino Acids

23.35

Weight (kDa)

5.34

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52300
fragaria_vesca FvH4_7g20110
malus_domestica MD01G1130700.v1.1 MD07G1178800.v1.1
prunus_persica Prupe.2G217900_v2.0.a1
pyrus_communis pycom01g13950 pycom07g17250
rosa_chinensis RchiOBHm_Chr1g0364071
rosa_laevigata RLG00000027519
rosa_multiflora Rmu_sc0000071.1_g000026
rosa_roxburghii Rroxscaffold_4G00292670
rosa_rugosa Rorug01G0313300
rosa_samantha Rh1AG320700 Rh1CG300300
rosa_wichuraiana Rw1G028430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 166
AccB1I GGYRCC 1 cut(s) 128
AccB7I CCANNNNNTGG 1 cut(s) 309
AccBSI CCGCTC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 396
AccII CGCG 2 cut(s) 16, 246
AciI CCGC 2 cut(s) 126, 531
AcoI YGGCCR 4 cut(s) 27, 96, 144, 192
AcsI RAATTY 1 cut(s) 572
AcuI CTGAAG 1 cut(s) 584
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 4 cut(s) 317, 356, 416, 542
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 14
AgsI TTSAA 4 cut(s) 160, 380, 494, 508
AjnI CCWGG 1 cut(s) 288
AluBI AGCT 2 cut(s) 199, 562
AluI AGCT 2 cut(s) 199, 562
Alw26I GTCTC 2 cut(s) 211, 342
Ama87I CYCGRG 2 cut(s) 166, 557
AoxI GGCC 5 cut(s) 27, 96, 144, 162, 192
ApeKI GCWGC 3 cut(s) 35, 38, 41
ApoI RAATTY 1 cut(s) 572
Asp700I GAANNNNTTC 1 cut(s) 572
AspLEI GCGC 2 cut(s) 131, 248
AsuC2I CCSGG 1 cut(s) 133
AvaI CYCGRG 2 cut(s) 166, 557
BalI TGGCCA 2 cut(s) 98, 194
BanI GGYRCC 1 cut(s) 128
BbvI GCAGC 3 cut(s) 47, 50, 53
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 1 cut(s) 290
BclI TGATCA 1 cut(s) 430
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 211, 342
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 132
BglI GCCNNNNNGGC 1 cut(s) 152
BisI GCNGC 4 cut(s) 36, 39, 42, 127
BlsI GCNGC 4 cut(s) 37, 40, 43, 128
BmcAI AGTACT 2 cut(s) 317, 542
Bme1390I CCNGG 2 cut(s) 133, 290
BmeT110I CYCGRG 2 cut(s) 166, 557
BmiI GGNNCC 2 cut(s) 130, 294
BmrFI CCNGG 2 cut(s) 133, 290
BmrI ACTGGG 3 cut(s) 190, 319, 610
BmsI GCATC 2 cut(s) 77, 562
BmuI ACTGGG 3 cut(s) 190, 319, 610
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpmI CTGGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 444
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse1I ACTGG 3 cut(s) 185, 314, 605
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 2 cut(s) 68, 553
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 3 cut(s) 185, 314, 605
BseRI GAGGAG 2 cut(s) 191, 461
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 3 cut(s) 47, 50, 53
Bsh1236I CGCG 2 cut(s) 16, 246
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 5 cut(s) 29, 98, 146, 164, 194
BshNI GGYRCC 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 30
BsiHKCI CYCGRG 2 cut(s) 166, 557
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 309
BsmAI GTCTC 2 cut(s) 211, 342
BsmBI CGTCTC 1 cut(s) 211
BsnI GGCC 5 cut(s) 29, 98, 146, 164, 194
Bso31I GGTCTC 1 cut(s) 342
BsoBI CYCGRG 2 cut(s) 166, 557
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 2 cut(s) 73, 430
BspACI CCGC 2 cut(s) 126, 531
BspANI GGCC 5 cut(s) 29, 98, 146, 164, 194
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 2 cut(s) 16, 246
BspLI GGNNCC 2 cut(s) 130, 294
BspT107I GGYRCC 1 cut(s) 128
BspTNI GGTCTC 1 cut(s) 342
BsrBI CCGCTC 1 cut(s) 126
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 3 cut(s) 185, 314, 605
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 2 cut(s) 73, 430
BssNI GRCGYC 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 1 cut(s) 243
BstACI GRCGYC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 2 cut(s) 100, 220
BstDEI CTNAG 1 cut(s) 411
BstF5I GGATG 2 cut(s) 68, 553
BstFNI CGCG 2 cut(s) 16, 246
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 2 cut(s) 131, 248
BstKTI GATC 2 cut(s) 76, 433
BstMAI GTCTC 2 cut(s) 211, 342
BstMBI GATC 2 cut(s) 73, 430
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 5 cut(s) 35, 41, 152, 161, 559
BstNI CCWGG 1 cut(s) 290
BstSCI CCNGG 2 cut(s) 131, 288
BstSFI CTRYAG 1 cut(s) 319
BstUI CGCG 2 cut(s) 16, 246
BstV1I GCAGC 3 cut(s) 47, 50, 53
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 5 cut(s) 29, 98, 146, 164, 194
BtsCI GGATG 2 cut(s) 68, 553
Cac8I GCNNGC 2 cut(s) 100, 220
CfoI GCGC 2 cut(s) 131, 248
Csp6I GTAC 4 cut(s) 316, 355, 415, 541
CviAII CATG 2 cut(s) 190, 526
CviQI GTAC 4 cut(s) 316, 355, 415, 541
DdeI CTNAG 1 cut(s) 411
DinI GGCGCC 1 cut(s) 130
DpnI GATC 2 cut(s) 75, 432
DpnII GATC 2 cut(s) 73, 430
DraI TTTAAA 1 cut(s) 459
EaeI YGGCCR 4 cut(s) 27, 96, 144, 192
EagI CGGCCG 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
EclXI CGGCCG 1 cut(s) 27
Eco147I AGGCCT 1 cut(s) 164
Eco31I GGTCTC 1 cut(s) 342
Eco52I CGGCCG 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 584
Eco88I CYCGRG 2 cut(s) 166, 557
EcoRII CCWGG 1 cut(s) 288
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 2 cut(s) 193, 529
FaiI YATR 7 cut(s) 87, 191, 305, 353, 369, 393, 527
FatI CATG 2 cut(s) 189, 525
FbaI TGATCA 1 cut(s) 430
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 4 cut(s) 36, 39, 42, 127
FokI GGATG 2 cut(s) 55, 540
Fsp4HI GCNGC 4 cut(s) 36, 39, 42, 127
GlaI GCGC 2 cut(s) 130, 247
GluI GCNGC 4 cut(s) 36, 39, 42, 127
GsuI CTGGAG 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 5 cut(s) 29, 98, 146, 164, 194
HapII CCGG 1 cut(s) 132
HhaI GCGC 2 cut(s) 131, 248
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 2 cut(s) 193, 529
Hin6I GCGC 2 cut(s) 129, 246
HinP1I GCGC 2 cut(s) 129, 246
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 2 cut(s) 82, 464
HpaII CCGG 1 cut(s) 132
Hpy166II GTNNAC 2 cut(s) 262, 397
Hpy188I TCNGA 5 cut(s) 73, 81, 211, 469, 590
Hpy188III TCNNGA 2 cut(s) 274, 557
Hpy8I GTNNAC 2 cut(s) 262, 397
Hpy99I CGWCG 2 cut(s) 246, 257
HpyAV CCTTC 2 cut(s) 33, 259
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4V TGCA 1 cut(s) 499
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 41, 152, 161, 559
HpyF3I CTNAG 1 cut(s) 411
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 2 cut(s) 193, 529
HspAI GCGC 2 cut(s) 129, 246
KasI GGCGCC 1 cut(s) 128
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 2 cut(s) 73, 430
LmnI GCTCC 3 cut(s) 204, 292, 469
Lsp1109I GCAGC 3 cut(s) 47, 50, 53
LweI GCATC 2 cut(s) 77, 562
MaeIII GTNAC 2 cut(s) 10, 403
MalI GATC 2 cut(s) 75, 432
MbiI CCGCTC 1 cut(s) 126
MboI GATC 2 cut(s) 73, 430
MboII GAAGA 5 cut(s) 148, 230, 520, 577, 580
MfeI CAATTG 1 cut(s) 434
MlsI TGGCCA 2 cut(s) 98, 194
MluCI AATT 3 cut(s) 330, 434, 572
MluI ACGCGT 1 cut(s) 14
MluNI TGGCCA 2 cut(s) 98, 194
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 447
Mox20I TGGCCA 2 cut(s) 98, 194
MroXI GAANNNNTTC 1 cut(s) 572
MscI TGGCCA 2 cut(s) 98, 194
MseI TTAA 1 cut(s) 458
Msp20I TGGCCA 2 cut(s) 98, 194
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 133, 290
MunI CAATTG 1 cut(s) 434
MvaI CCWGG 1 cut(s) 290
MvnI CGCG 2 cut(s) 16, 246
MwoI GCNNNNNNNGC 5 cut(s) 35, 41, 152, 161, 559
NarI GGCGCC 1 cut(s) 129
NciI CCSGG 1 cut(s) 133
NdeII GATC 2 cut(s) 73, 430
NlaIII CATG 2 cut(s) 193, 529
NlaIV GGNNCC 2 cut(s) 130, 294
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 403
PaeR7I CTCGAG 1 cut(s) 166
PceI AGGCCT 1 cut(s) 164
PdmI GAANNNNTTC 1 cut(s) 572
PfeI GAWTC 1 cut(s) 82
PflFI GACNNNGTC 2 cut(s) 257, 401
PflMI CCANNNNNTGG 1 cut(s) 309
PkrI GCNGC 4 cut(s) 37, 40, 43, 128
PleI GAGTC 1 cut(s) 472
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 130, 294
PspXI VCTCGAGB 1 cut(s) 166
PsyI GACNNNGTC 2 cut(s) 257, 401
RsaI GTAC 4 cut(s) 317, 356, 416, 542
RsaNI GTAC 4 cut(s) 316, 355, 415, 541
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 4 cut(s) 36, 39, 42, 127
Sau3AI GATC 2 cut(s) 73, 430
ScaI AGTACT 2 cut(s) 317, 542
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 2 cut(s) 133, 290
SetI ASST 4 cut(s) 201, 261, 412, 564
SfaNI GCATC 2 cut(s) 77, 562
SfcI CTRYAG 1 cut(s) 319
SfoI GGCGCC 1 cut(s) 130
Sfr274I CTCGAG 1 cut(s) 166
SlaI CTCGAG 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 3 cut(s) 330, 434, 572
SseBI AGGCCT 1 cut(s) 164
SsiI CCGC 2 cut(s) 126, 531
SspDI GGCGCC 1 cut(s) 128
StuI AGGCCT 1 cut(s) 164
StyD4I CCNGG 2 cut(s) 131, 288
TaaI ACNGT 1 cut(s) 403
TaqI TCGA 4 cut(s) 167, 177, 252, 576
TasI AATT 3 cut(s) 330, 434, 572
TatI WGTACW 4 cut(s) 315, 354, 414, 540
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 3 cut(s) 35, 38, 41
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 2 cut(s) 74, 597
Tth111I GACNNNGTC 2 cut(s) 257, 401
Van91I CCANNNNNTGG 1 cut(s) 309
XapI RAATTY 1 cut(s) 572
XhoI CTCGAG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 572
ZrmI AGTACT 2 cut(s) 317, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.