AT3G52500

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015999
Physical Location & Seq
Reverse (-)
19465264 .. 19467455
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G52500.1

Sequence Viewer

Length: 1410 bp
ATGGCTTCTTCTATCTTCTTCTTCTTCCTTATCTTCCTCTCCGTCGTCTCCGCCGTCAAACTACCTCTCTCTCCCTTCTCCCACTCCGACCAATCACCAAAAGACCCTTACCTCTCTCTCCGTCGTCTCGCTGAATCCTCAATCGCTAGAGCTCATAAACTCAAACACGGCACCTCAATAAAACCCGACGAAGATGCTCTCTCCTCCACCACCACCGCATCCGCCACCGTCGTGAAATCTCCTCTCTCTGCTAAAAGCTACGGCGGATACTCAGTCTCTCTCAGCTTCGGCACACCGTCGCAAACAATCCCATTCGTTTTCGATACCGGAAGCAGTCTCGTTTGGCTACCTTGTACCTCAAGATATCTCTGCTCCGGTTGCGATTTCTCCGGTTTGGATCCGACTCTAATCCCTAGATTTATCCCCAAGAACTCTTCCTCTTCGAAGATTATCGGGTGTCAGAGTCCAAAATGTCAGTTTCTATACGGACCCAATGTTCAATGCAGAGGATGTGACCCGAATACCCGAAACTGCACCGTCGGTTGTCCACCGTACATTCTTCAATACGGATTAGGATCAACGGCTGGGGTTTTAATAACCGAAAAGCTTGATTTCCCGGATCTAACCGTACCCGATTTCGTAGTCGGATGTTCAATTATCTCCACCCGACAACCCGCTGGTATCGCTGGGTTTGGGCGAGGACCCGTATCGCTTCCTTCACAAATGAATCTCAAGAGATTCTCTCACTGTTTGGTTTCTCGTCGGTTTGACGATACCAACGTAACCACCGATCTTGATTTAGATACCGGATCGGGTCATAATTCCGGGTCTAAAACTCCGGGTCTCACATACACGCCGTTTCGGAAAAATCCCAATGTCTCCAACAAAGCTTTTCTCGAGTATTATTACCTCAATCTCCGGCGAATCTACGTCGGAAGAAAGCACGTGAAGATTCCGTATAAGTATCTCGCGCCGGGAACTAACGGAGACGGAGGATCCATTGTTGATTCCGGATCTACATTCACTTTCATGGAGCGACCCGTTTTCGAGCTCGTTGCTGAAGAATTCGCTTCACAGATGTCGAACTACACTCGTGAGAAAGACTTGGAGAAGGAAACAGGACTTGGACCGTGTTTTAATATCTCCGGGAAGGGAGATGTGACGGTGCCGGAGCTGATTTTTGAGTTTAAAGGTGGAGCGAAGTTGGAATTGCCGTTATCCAATTACTTCACGTTCGTTGGGAATACTGATACCGTTTGTCTTACGGTGGTTTCGGATAAAACCGTTAATCCCTCCGGCGGGACTGGACCGGCGATAATTCTGGGGAGTTTCCAGCAACAGAACTATTTAGTTGAATACGATTTGGAGAATGACAGGTTTGGGTTCGCGAAGAAGAAGTGTAGCCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

469

Amino Acids

51.0

Weight (kDa)

8.53

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 90 - 254 4.1e-28 Xylanase inhibitor N-terminal
Asp PF00026 90 - 151 8.5e-06 Eukaryotic aspartyl protease
Asp PF00026 187 - 464 6.2e-09 Eukaryotic aspartyl protease
TAXi_C PF14541 302 - 464 1.8e-33 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52500
fragaria_vesca FvH4_6g20050
malus_domestica MD12G1037600.v1.1 MD14G1036600.v1.1
prunus_persica Prupe.7G097600_v2.0.a1
pyrus_communis pycom12g03260
rosa_chinensis RchiOBHm_Chr3g0475521
rosa_laevigata RLG00000023836
rosa_roxburghii Rroxscaffold_6G00406210
rosa_rugosa Rorug03G0150100
rosa_samantha Rh3AG199700 Rh3BG229700 Rh3CG224000 Rh3DG225000
rosa_wichuraiana Rw3G018100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 170, 1165
AccII CGCG 2 cut(s) 971, 1388
AccIII TCCGGA 1 cut(s) 1010
AciI CCGC 6 cut(s) 51, 216, 222, 264, 675, 1299
AclWI GGATC 8 cut(s) 392, 405, 583, 627, 817, 990, 1003, 1021
AcsI RAATTY 1 cut(s) 1064
AcuI CTGAAG 1 cut(s) 1080
AcvI CACGTG 1 cut(s) 946
AfaI GTAC 3 cut(s) 355, 554, 630
AfiI CCNNNNNNNGG 2 cut(s) 1298, 1299
AgsI TTSAA 4 cut(s) 500, 563, 654, 1355
AjuI GAANNNNNNNTTGG 2 cut(s) 1107, 1139
AleI CACNNNNGTG 1 cut(s) 230
AloI GAACNNNNNNTCC 2 cut(s) 480, 512
AluBI AGCT 7 cut(s) 152, 258, 285, 607, 890, 1051, 1174
AluI AGCT 7 cut(s) 152, 258, 285, 607, 890, 1051, 1174
Alw21I GWGCWC 2 cut(s) 154, 1053
Alw26I GTCTC 7 cut(s) 52, 131, 280, 341, 848, 883, 981
AlwI GGATC 8 cut(s) 392, 405, 583, 627, 817, 990, 1003, 1021
Ama87I CYCGRG 1 cut(s) 896
Aor13HI TCCGGA 1 cut(s) 1010
ApoI RAATTY 1 cut(s) 1064
AspLEI GCGC 1 cut(s) 973
AspS9I GGNCC 4 cut(s) 488, 701, 1127, 1307
AsuC2I CCSGG 5 cut(s) 617, 826, 840, 975, 1147
AsuHPI GGTGA 1 cut(s) 87
AsuII TTCGAA 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 896
AvaII GGWCC 4 cut(s) 488, 701, 1127, 1307
BaeI ACNNNNGTAYC 2 cut(s) 765, 798
BamHI GGATCC 2 cut(s) 397, 995
BanI GGYRCC 2 cut(s) 170, 1165
BanII GRGCYC 2 cut(s) 154, 1053
BauI CACGAG 1 cut(s) 1092
BbrPI CACGTG 1 cut(s) 946
Bbv12I GWGCWC 2 cut(s) 154, 1053
BceAI ACGGC 6 cut(s) 38, 184, 277, 597, 841, 1198
BcgI CGANNNNNNTGC 4 cut(s) 176, 210, 1037, 1071
BciVI GTATCC 1 cut(s) 260
BcnI CCSGG 5 cut(s) 617, 826, 840, 975, 1147
BcoDI GTCTC 7 cut(s) 52, 131, 280, 341, 848, 883, 981
BfaI CTAG 2 cut(s) 147, 414
BfuI GTATCC 1 cut(s) 260
Bme1390I CCNGG 5 cut(s) 617, 826, 840, 975, 1147
Bme18I GGWCC 4 cut(s) 488, 701, 1127, 1307
BmeT110I CYCGRG 1 cut(s) 896
BmgT120I GGNCC 4 cut(s) 488, 701, 1127, 1307
BmiI GGNNCC 6 cut(s) 172, 399, 490, 703, 997, 1167
BmrFI CCNGG 5 cut(s) 617, 826, 840, 975, 1147
BmsI GCATC 2 cut(s) 184, 227
BplI GAGNNNNNCTC 2 cut(s) 727, 759
Bpu14I TTCGAA 1 cut(s) 443
BpuEI CTTGAG 2 cut(s) 343, 716
BpuMI CCSGG 5 cut(s) 617, 826, 840, 975, 1147
BsaAI YACGTR 1 cut(s) 946
BsaBI GATNNNNATC 1 cut(s) 574
BsaI GGTCTC 1 cut(s) 848
BsaWI WCCGGW 5 cut(s) 326, 374, 389, 806, 1010
Bsc4I CCNNNNNNNGG 2 cut(s) 1298, 1299
Bse118I RCCGGY 1 cut(s) 1309
Bse1I ACTGG 1 cut(s) 1309
Bse8I GATNNNNATC 1 cut(s) 574
BseAI TCCGGA 1 cut(s) 1010
BseGI GGATG 3 cut(s) 218, 515, 653
BseJI GATNNNNATC 1 cut(s) 574
BseLI CCNNNNNNNGG 2 cut(s) 1298, 1299
BseMII CTCAG 2 cut(s) 285, 295
BseNI ACTGG 1 cut(s) 1309
BseRI GAGGAG 2 cut(s) 193, 231
BseYI CCCAGC 2 cut(s) 584, 686
BsgI GTGCAG 1 cut(s) 517
Bsh1236I CGCG 2 cut(s) 971, 1388
BshNI GGYRCC 2 cut(s) 170, 1165
BsiHKAI GWGCWC 2 cut(s) 154, 1053
BsiHKCI CYCGRG 1 cut(s) 896
BslFI GGGAC 1 cut(s) 1315
BslI CCNNNNNNNGG 2 cut(s) 1298, 1299
BsmAI GTCTC 7 cut(s) 52, 131, 280, 341, 848, 883, 981
BsmBI CGTCTC 3 cut(s) 52, 131, 981
BsmFI GGGAC 1 cut(s) 1315
Bso31I GGTCTC 1 cut(s) 848
BsoBI CYCGRG 1 cut(s) 896
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 2 cut(s) 154, 1053
Bsp13I TCCGGA 1 cut(s) 1010
Bsp143I GATC 7 cut(s) 397, 575, 619, 790, 809, 995, 1013
Bsp68I TCGCGA 1 cut(s) 1388
BspACI CCGC 6 cut(s) 51, 216, 222, 264, 675, 1299
BspCNI CTCAG 2 cut(s) 284, 294
BspEI TCCGGA 1 cut(s) 1010
BspFNI CGCG 2 cut(s) 971, 1388
BspLI GGNNCC 6 cut(s) 172, 399, 490, 703, 997, 1167
BspPI GGATC 8 cut(s) 392, 405, 583, 627, 817, 990, 1003, 1021
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 2 cut(s) 170, 1165
BspTNI GGTCTC 1 cut(s) 848
BsrFI RCCGGY 1 cut(s) 1309
BsrI ACTGG 1 cut(s) 1309
BssAI RCCGGY 1 cut(s) 1309
BssMI GATC 7 cut(s) 397, 575, 619, 790, 809, 995, 1013
BssSI CACGAG 1 cut(s) 1092
Bst2BI CACGAG 1 cut(s) 1092
Bst6I CTCTTC 2 cut(s) 439, 445
BstBAI YACGTR 1 cut(s) 946
BstBI TTCGAA 1 cut(s) 443
BstDEI CTNAG 2 cut(s) 271, 281
BstF5I GGATG 3 cut(s) 218, 515, 653
BstFNI CGCG 2 cut(s) 971, 1388
BstHHI GCGC 1 cut(s) 973
BstKTI GATC 7 cut(s) 400, 578, 622, 793, 812, 998, 1016
BstMAI GTCTC 7 cut(s) 52, 131, 280, 341, 848, 883, 981
BstMBI GATC 7 cut(s) 397, 575, 619, 790, 809, 995, 1013
BstMWI GCNNNNNNNGC 2 cut(s) 378, 683
BstSCI CCNGG 5 cut(s) 615, 824, 838, 973, 1145
BstUI CGCG 2 cut(s) 971, 1388
BstX2I RGATCY 4 cut(s) 397, 619, 995, 1013
BstYI RGATCY 4 cut(s) 397, 619, 995, 1013
BsuI GTATCC 1 cut(s) 260
BtsCI GGATG 3 cut(s) 218, 515, 653
BtsIMutI CAGTG 1 cut(s) 745
BtuMI TCGCGA 1 cut(s) 1388
CfoI GCGC 1 cut(s) 973
Cfr10I RCCGGY 1 cut(s) 1309
Cfr13I GGNCC 4 cut(s) 488, 701, 1127, 1307
Csp6I GTAC 3 cut(s) 354, 553, 629
CspCI CAANNNNNGTGG 2 cut(s) 775, 810
CviAII CATG 1 cut(s) 1030
CviQI GTAC 3 cut(s) 354, 553, 629
DdeI CTNAG 2 cut(s) 271, 281
DpnI GATC 7 cut(s) 399, 577, 621, 792, 811, 997, 1015
DpnII GATC 7 cut(s) 397, 575, 619, 790, 809, 995, 1013
DraI TTTAAA 1 cut(s) 1189
Eam1104I CTCTTC 2 cut(s) 439, 445
EarI CTCTTC 2 cut(s) 439, 445
EciI GGCGGA 3 cut(s) 40, 211, 279
Ecl136II GAGCTC 2 cut(s) 152, 1051
Eco24I GRGCYC 2 cut(s) 154, 1053
Eco31I GGTCTC 1 cut(s) 848
Eco32I GATATC 1 cut(s) 365
Eco47I GGWCC 4 cut(s) 488, 701, 1127, 1307
Eco53kI GAGCTC 2 cut(s) 152, 1051
Eco57I CTGAAG 1 cut(s) 1080
Eco72I CACGTG 1 cut(s) 946
Eco88I CYCGRG 1 cut(s) 896
EcoICRI GAGCTC 2 cut(s) 152, 1051
EcoO109I RGGNCCY 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 1064
EcoRV GATATC 1 cut(s) 365
EcoT38I GRGCYC 2 cut(s) 154, 1053
Esp3I CGTCTC 3 cut(s) 52, 131, 981
FaeI CATG 1 cut(s) 1033
FaiI YATR 6 cut(s) 156, 484, 819, 850, 960, 1031
FaqI GGGAC 1 cut(s) 1315
FatI CATG 1 cut(s) 1029
FauI CCCGC 2 cut(s) 682, 1292
FokI GGATG 3 cut(s) 205, 522, 660
FriOI GRGCYC 2 cut(s) 154, 1053
FspBI CTAG 2 cut(s) 147, 414
GlaI GCGC 1 cut(s) 972
GsaI CCCAGC 2 cut(s) 588, 690
HhaI GCGC 1 cut(s) 973
Hin1II CATG 1 cut(s) 1033
Hin6I GCGC 1 cut(s) 971
HinP1I GCGC 1 cut(s) 971
HindIII AAGCTT 2 cut(s) 605, 888
HinfI GANTC 8 cut(s) 134, 403, 463, 727, 738, 924, 952, 1007
HphI GGTGA 1 cut(s) 87
Hpy166II GTNNAC 1 cut(s) 548
Hpy188I TCNGA 7 cut(s) 88, 402, 462, 647, 864, 935, 1276
Hpy188III TCNNGA 8 cut(s) 232, 360, 733, 794, 896, 1011, 1094, 1387
Hpy8I GTNNAC 1 cut(s) 548
Hpy99I CGWCG 8 cut(s) 47, 126, 191, 233, 301, 542, 765, 935
HpyAV CCTTC 4 cut(s) 85, 726, 1105, 1144
HpyCH4IV ACGT 4 cut(s) 780, 930, 945, 1232
HpyCH4V TGCA 2 cut(s) 504, 534
HpyF10VI GCNNNNNNNGC 2 cut(s) 378, 683
HpyF3I CTNAG 2 cut(s) 271, 281
HpySE526I ACGT 4 cut(s) 780, 930, 945, 1232
Hsp92II CATG 1 cut(s) 1033
HspAI GCGC 1 cut(s) 971
Kpn2I TCCGGA 1 cut(s) 1010
Kzo9I GATC 7 cut(s) 397, 575, 619, 790, 809, 995, 1013
LmnI GCTCC 4 cut(s) 377, 1033, 1171, 1196
LweI GCATC 2 cut(s) 184, 227
MaeI CTAG 2 cut(s) 147, 414
MaeII ACGT 4 cut(s) 780, 930, 945, 1232
MaeIII GTNAC 3 cut(s) 512, 781, 1159
MalI GATC 7 cut(s) 399, 577, 621, 792, 811, 997, 1015
MboI GATC 7 cut(s) 397, 575, 619, 790, 809, 995, 1013
MflI RGATCY 4 cut(s) 397, 619, 995, 1013
MhlI GDGCHC 2 cut(s) 154, 1053
MluCI AATT 6 cut(s) 654, 820, 1064, 1208, 1222, 1317
MlyI GAGTC 2 cut(s) 397, 472
MmeI TCCRAC 6 cut(s) 111, 425, 625, 906, 913, 1185
MroI TCCGGA 1 cut(s) 1010
MseI TTAA 4 cut(s) 593, 1137, 1188, 1287
MslI CAYNNNNRTG 2 cut(s) 230, 1028
MspA1I CMGCKG 1 cut(s) 677
MspR9I CCNGG 5 cut(s) 617, 826, 840, 975, 1147
MvnI CGCG 2 cut(s) 971, 1388
MwoI GCNNNNNNNGC 2 cut(s) 378, 683
NciI CCSGG 5 cut(s) 617, 826, 840, 975, 1147
NdeII GATC 7 cut(s) 397, 575, 619, 790, 809, 995, 1013
NlaIII CATG 1 cut(s) 1033
NlaIV GGNNCC 6 cut(s) 172, 399, 490, 703, 997, 1167
NmuCI GTSAC 2 cut(s) 512, 1159
NruI TCGCGA 1 cut(s) 1388
NspV TTCGAA 1 cut(s) 443
OliI CACNNNNGTG 1 cut(s) 230
PaeR7I CTCGAG 1 cut(s) 896
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 777, 786
PfeI GAWTC 6 cut(s) 134, 727, 738, 924, 952, 1007
PfoI TCCNGGA 2 cut(s) 615, 1145
PleI GAGTC 2 cut(s) 397, 471
PmaCI CACGTG 1 cut(s) 946
PmlI CACGTG 1 cut(s) 946
PpsI GAGTC 2 cut(s) 397, 471
Ppu21I YACGTR 1 cut(s) 946
PpuMI RGGWCCY 1 cut(s) 701
Psp124BI GAGCTC 2 cut(s) 154, 1053
Psp5II RGGWCCY 1 cut(s) 701
PspCI CACGTG 1 cut(s) 946
PspFI CCCAGC 2 cut(s) 584, 686
PspN4I GGNNCC 6 cut(s) 172, 399, 490, 703, 997, 1167
PspPI GGNCC 4 cut(s) 488, 701, 1127, 1307
PspPPI RGGWCCY 1 cut(s) 701
PsuI RGATCY 4 cut(s) 397, 619, 995, 1013
RruI TCGCGA 1 cut(s) 1388
RsaI GTAC 3 cut(s) 355, 554, 630
RsaNI GTAC 3 cut(s) 354, 553, 629
RseI CAYNNNNRTG 2 cut(s) 230, 1028
SacI GAGCTC 2 cut(s) 154, 1053
SaqAI TTAA 4 cut(s) 593, 1137, 1188, 1287
Sau3AI GATC 7 cut(s) 397, 575, 619, 790, 809, 995, 1013
Sau96I GGNCC 4 cut(s) 488, 701, 1127, 1307
SchI GAGTC 2 cut(s) 397, 472
ScrFI CCNGG 5 cut(s) 617, 826, 840, 975, 1147
SduI GDGCHC 2 cut(s) 154, 1053
SfaNI GCATC 2 cut(s) 184, 227
Sfr274I CTCGAG 1 cut(s) 896
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 4 cut(s) 488, 701, 1127, 1307
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 2 cut(s) 230, 1028
SmlI CTYRAG 3 cut(s) 358, 731, 896
SmoI CTYRAG 3 cut(s) 358, 731, 896
Sse9I AATT 6 cut(s) 654, 820, 1064, 1208, 1222, 1317
SsiI CCGC 6 cut(s) 51, 216, 222, 264, 675, 1299
SspMI CTAG 2 cut(s) 147, 414
SstI GAGCTC 2 cut(s) 154, 1053
StyD4I CCNGG 5 cut(s) 615, 824, 838, 973, 1145
TaiI ACGT 4 cut(s) 783, 933, 948, 1235
TaqI TCGA 5 cut(s) 321, 443, 897, 1047, 1082
TasI AATT 6 cut(s) 654, 820, 1064, 1208, 1222, 1317
TfiI GAWTC 6 cut(s) 134, 727, 738, 924, 952, 1007
Tru1I TTAA 4 cut(s) 593, 1137, 1188, 1287
Tru9I TTAA 4 cut(s) 593, 1137, 1188, 1287
TscAI CASTG 1 cut(s) 752
TseFI GTSAC 2 cut(s) 512, 1159
Tsp45I GTSAC 2 cut(s) 512, 1159
TspDTI ATGAA 2 cut(s) 740, 1018
TspGWI ACGGA 7 cut(s) 31, 110, 501, 582, 945, 999, 1005
TspRI CASTG 1 cut(s) 752
VpaK11BI GGWCC 4 cut(s) 488, 701, 1127, 1307
XapI RAATTY 1 cut(s) 1064
XhoI CTCGAG 1 cut(s) 896
XspI CTAG 2 cut(s) 147, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.