Rw3G018100

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0015999
Physical Location & Seq
Forward (+)
20029628 .. 20031000
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G018100.1

Sequence Viewer

Length: 1335 bp
ATGGCGACTCCCAATCCCCTCCTCCTCTCTCTCATTTCCCTCTTCTCTCTTTTCCACCTCGCATTCTCATCCAAACTCACACTTCCTCTCTCCCTACTCGCCACACACCACCCCTCCTCAGATCCAATCCAGACCCTCAGCCTCCTCTCCTCCGCCTCACTCTCCCGCGCCCACCACCTCAAACGCCCCAAACACTCCAACTCCTCCACCACCAAAGTCCCTCTCTACCCCCGCGGCTTCGGCGCCTACTCCATTCCCCTCAGCTTCGGCACGCCGCCGCAGACCTCCACCTTCCTCATGGACACCGGCAGCAGCCTCGTCTGGTTCCCCTGCACCTCCCGCTATAGCTGCTCCAGCTGCGTCTTCCCCAACATTGACCCCACCCACATCCCCACCTTCATCCCCAAGCTCTCCTCCACTTCCCGAATCGTCGGCTGCAAAAACCCAAAATGCGCCTGGATTTTCGGCCCGCAAATCAATACCAAATGTCCAAACTCCTCCCAGACCTGCCCGACGTACGTCATCCAGTACGGCTCCGGCGCCACCGCCGGAGTTTTGCTCTCCGAGTCCCTCGATTTACCCAATAAAACCGTCCCCGATTTCCTCGTCGGCTGCTCTTTCCTCTCGATCCGACAACCCGCCGGAATCGCCGGGTTCGGACGCGGCCCGGAATCCCTCCCCGTCCAAATGGGCCTCTCCAAATTCTCCTACTGCCTAGTCTCCCGCAGATTCGACGACACCCCCGTCAGCAGCGACCTCGTTCTCTACAGCGGCTCCACCTCCGACGACCATGACGACAGTGGTGGCGTCAGCTACACTCCTTTCCAGAAAAACCCCGGCGCCTCCAACTCCGCCTACCGCGAGTACTACTACCTCGCTCTCCGCAAAGTCGTCGTCGGGAAAAAACACGTCAAGCTACCTTACAAGTACTTGGTTCCGGGGGCCGACGACAATGGCGGGACCATCGTGGACTCCGGGTCGACGTTCACGTTCATGGAGAGGCCGGTGTTCGAGGCGGTGGCGGAGGCGTTCGCGGCGCAGATGGTGAAGTACACCCGGGCCGAGGACATAGAGAACCGGACCGGGTTGAAGCCGTGCTTTGATATTTCCAAGGAGAAAACGGTGGACTTCCCGGAGCTGGTTTTCCAATTCAAGGGCGGTGCGAAGATGGCATTGCCGTTGAGCAATTACTTCGCTCTGGTCACCAGCGGCGGCGTTGTCGTGCCGACTGGGCCCGCGATTGTTTTGGGGAGTTTCCAGCAGCAGAATTTTTACGTCGAGTACGATTTGGCGCACGATAGGTTCGGGTTTAGGAAACAGAGCTGTAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

444

Amino Acids

48.35

Weight (kDa)

8.97

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 83 - 241 5.3e-28 Xylanase inhibitor N-terminal
Asp PF00026 83 - 437 5.4e-16 Eukaryotic aspartyl protease
TAXi_C PF14541 290 - 438 8.1e-27 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52500
fragaria_vesca FvH4_6g20050
malus_domestica MD12G1037600.v1.1 MD14G1036600.v1.1
prunus_persica Prupe.7G097600_v2.0.a1
pyrus_communis pycom12g03260
rosa_chinensis RchiOBHm_Chr3g0475521
rosa_laevigata RLG00000023836
rosa_roxburghii Rroxscaffold_6G00406210
rosa_rugosa Rorug03G0150100
rosa_samantha Rh3AG199700 Rh3BG229700 Rh3CG224000 Rh3DG225000
rosa_wichuraiana Rw3G018100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 743
Acc36I ACCTGC 1 cut(s) 515
AccB1I GGYRCC 3 cut(s) 242, 539, 839
AccI GTMKAC 1 cut(s) 980
AccII CGCG 6 cut(s) 168, 234, 663, 861, 1034, 1238
AclWI GGATC 2 cut(s) 116, 622
AcsI RAATTY 2 cut(s) 701, 1267
AcyI GRCGYC 4 cut(s) 243, 540, 807, 840
AfaI GTAC 6 cut(s) 518, 530, 866, 929, 1052, 1283
AfiI CCNNNNNNNGG 3 cut(s) 1063, 1138, 1153
AflIII ACRYGT 1 cut(s) 907
AgsI TTSAA 2 cut(s) 1090, 1153
AhdI GACNNNNNGTC 1 cut(s) 976
AjiI CACGTC 1 cut(s) 910
AjnI CCWGG 1 cut(s) 455
AluBI AGCT 8 cut(s) 264, 348, 357, 409, 813, 916, 1138, 1323
AluI AGCT 8 cut(s) 264, 348, 357, 409, 813, 916, 1138, 1323
Alw26I GTCTC 1 cut(s) 724
AlwI GGATC 2 cut(s) 116, 622
Ama87I CYCGRG 1 cut(s) 1056
AoxI GGCC 7 cut(s) 466, 664, 691, 942, 1001, 1059, 1232
ApaI GGGCCC 1 cut(s) 1236
ApeKI GCWGC 8 cut(s) 309, 312, 348, 357, 435, 612, 750, 1261
ApoI RAATTY 2 cut(s) 701, 1267
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
AspLEI GCGC 7 cut(s) 170, 245, 455, 542, 842, 1039, 1294
AspS9I GGNCC 9 cut(s) 467, 665, 691, 942, 960, 1059, 1080, 1232, 1233
AsuC2I CCSGG 9 cut(s) 652, 668, 837, 939, 976, 1057, 1058, 1084, 1133
AsuHPI GGTGA 2 cut(s) 1057, 1195
AvaI CYCGRG 1 cut(s) 1056
AvaII GGWCC 2 cut(s) 960, 1080
BaeGI GKGCMC 1 cut(s) 1236
BanI GGYRCC 3 cut(s) 242, 539, 839
BanII GRGCYC 1 cut(s) 1236
BbsI GAAGAC 1 cut(s) 355
BbvCI CCTCAGC 2 cut(s) 137, 260
BbvI GCAGC 8 cut(s) 321, 324, 335, 344, 422, 599, 762, 1273
BccI CCATC 3 cut(s) 971, 1036, 1162
BceAI ACGGC 3 cut(s) 547, 1078, 1162
BcgI CGANNNNNNTGC 8 cut(s) 298, 332, 739, 773, 874, 908, 1174, 1208
BciT130I CCWGG 1 cut(s) 457
BcnI CCSGG 9 cut(s) 652, 668, 837, 939, 976, 1057, 1058, 1084, 1133
BcoDI GTCTC 1 cut(s) 724
BfaI CTAG 1 cut(s) 716
BfmI CTRYAG 2 cut(s) 343, 766
BfoI RGCGCY 3 cut(s) 246, 543, 843
BfuAI ACCTGC 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 1231
BmcAI AGTACT 2 cut(s) 866, 929
Bme18I GGWCC 2 cut(s) 960, 1080
BmeRI GACNNNNNGTC 1 cut(s) 976
BmeT110I CYCGRG 1 cut(s) 1056
BmgBI CACGTC 1 cut(s) 910
BmgT120I GGNCC 9 cut(s) 467, 665, 691, 942, 960, 1059, 1080, 1232, 1233
BmrI ACTGGG 1 cut(s) 1239
BmuI ACTGGG 1 cut(s) 1239
BoxI GACNNNNGTC 1 cut(s) 518
BpiI GAAGAC 1 cut(s) 355
BplI GAGNNNNNCTC 2 cut(s) 543, 575
BpmI CTGGAG 1 cut(s) 337
Bpu10I CCTNAGC 2 cut(s) 137, 260
BpuMI CCSGG 9 cut(s) 652, 668, 837, 939, 976, 1057, 1058, 1084, 1133
BsaHI GRCGYC 4 cut(s) 243, 540, 807, 840
BsaJI CCNNGG 6 cut(s) 232, 835, 938, 1056, 1062, 1110
BsaWI WCCGGW 1 cut(s) 1077
BsaXI ACNNNNNCTCC 6 cut(s) 98, 128, 185, 215, 758, 788
Bsc4I CCNNNNNNNGG 3 cut(s) 1063, 1138, 1153
Bse118I RCCGGY 2 cut(s) 305, 1003
Bse1I ACTGG 2 cut(s) 526, 1234
Bse3DI GCAATG 1 cut(s) 1172
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 6 cut(s) 232, 835, 938, 1056, 1062, 1110
BseGI GGATG 4 cut(s) 68, 387, 399, 522
BseLI CCNNNNNNNGG 3 cut(s) 1063, 1138, 1153
BseMI GCAATG 1 cut(s) 1172
BseMII CTCAG 3 cut(s) 132, 151, 274
BseNI ACTGG 2 cut(s) 526, 1234
BseRI GAGGAG 8 cut(s) 11, 14, 106, 134, 139, 193, 403, 487
BseSI GKGCMC 1 cut(s) 1236
BseXI GCAGC 8 cut(s) 321, 324, 335, 344, 422, 599, 762, 1273
BsgI GTGCAG 1 cut(s) 316
Bsh1236I CGCG 6 cut(s) 168, 234, 663, 861, 1034, 1238
BshFI GGCC 7 cut(s) 468, 666, 693, 944, 1003, 1061, 1234
BshNI GGYRCC 3 cut(s) 242, 539, 839
BsiHKCI CYCGRG 1 cut(s) 1056
BsiWI CGTACG 1 cut(s) 516
BslFI GGGAC 4 cut(s) 203, 553, 578, 973
BslI CCNNNNNNNGG 3 cut(s) 1063, 1138, 1153
BsmAI GTCTC 1 cut(s) 724
BsmFI GGGAC 4 cut(s) 203, 553, 578, 973
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 7 cut(s) 468, 666, 693, 944, 1003, 1061, 1234
BsoBI CYCGRG 1 cut(s) 1056
Bsp120I GGGCCC 1 cut(s) 1232
Bsp1286I GDGCHC 1 cut(s) 1236
Bsp143I GATC 2 cut(s) 121, 627
BspANI GGCC 7 cut(s) 468, 666, 693, 944, 1003, 1061, 1234
BspCNI CTCAG 3 cut(s) 131, 150, 273
BspFNI CGCG 6 cut(s) 168, 234, 663, 861, 1034, 1238
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 2 cut(s) 116, 622
BspT107I GGYRCC 3 cut(s) 242, 539, 839
BsrDI GCAATG 1 cut(s) 1172
BsrFI RCCGGY 2 cut(s) 305, 1003
BsrI ACTGG 2 cut(s) 526, 1234
BssAI RCCGGY 2 cut(s) 305, 1003
BssECI CCNNGG 6 cut(s) 232, 835, 938, 1056, 1062, 1110
BssMI GATC 2 cut(s) 121, 627
BssNI GRCGYC 4 cut(s) 243, 540, 807, 840
BssT1I CCWWGG 1 cut(s) 1110
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 3 cut(s) 592, 800, 1123
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 4 cut(s) 243, 540, 807, 840
BstC8I GCNNGC 3 cut(s) 272, 470, 1236
BstDEI CTNAG 3 cut(s) 118, 137, 260
BstDSI CCRYGG 1 cut(s) 232
BstEII GGTNACC 1 cut(s) 1201
BstF5I GGATG 4 cut(s) 68, 387, 399, 522
BstFNI CGCG 6 cut(s) 168, 234, 663, 861, 1034, 1238
BstH2I RGCGCY 3 cut(s) 246, 543, 843
BstHHI GCGC 7 cut(s) 170, 245, 455, 542, 842, 1039, 1294
BstKTI GATC 2 cut(s) 124, 630
BstMAI GTCTC 1 cut(s) 724
BstMBI GATC 2 cut(s) 121, 627
BstMWI GCNNNNNNNGC 9 cut(s) 240, 339, 348, 354, 357, 647, 1034, 1169, 1231
BstNI CCWGG 1 cut(s) 457
BstPAI GACNNNNGTC 1 cut(s) 518
BstPI GGTNACC 1 cut(s) 1201
BstSFI CTRYAG 2 cut(s) 343, 766
BstSLI GKGCMC 1 cut(s) 1236
BstUI CGCG 6 cut(s) 168, 234, 663, 861, 1034, 1238
BstV1I GCAGC 8 cut(s) 321, 324, 335, 344, 422, 599, 762, 1273
BstV2I GAAGAC 1 cut(s) 355
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 7 cut(s) 468, 666, 693, 944, 1003, 1061, 1234
BtgI CCRYGG 1 cut(s) 232
BtrI CACGTC 1 cut(s) 910
BtsCI GGATG 4 cut(s) 68, 387, 399, 522
BtsIMutI CAGTG 1 cut(s) 805
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 3 cut(s) 272, 470, 1236
CfoI GCGC 7 cut(s) 170, 245, 455, 542, 842, 1039, 1294
Cfr10I RCCGGY 2 cut(s) 305, 1003
Cfr13I GGNCC 9 cut(s) 467, 665, 691, 942, 960, 1059, 1080, 1232, 1233
Cfr42I CCGCGG 1 cut(s) 235
Cfr9I CCCGGG 1 cut(s) 1056
CpoI CGGWCCG 1 cut(s) 1080
CseI GACGC 3 cut(s) 349, 669, 796
Csp6I GTAC 6 cut(s) 517, 529, 865, 928, 1051, 1282
CspI CGGWCCG 1 cut(s) 1080
CviAII CATG 3 cut(s) 298, 791, 994
CviQI GTAC 6 cut(s) 517, 529, 865, 928, 1051, 1282
DdeI CTNAG 3 cut(s) 118, 137, 260
DinI GGCGCC 3 cut(s) 244, 541, 841
DpnI GATC 2 cut(s) 123, 629
DpnII GATC 2 cut(s) 121, 627
DrdI GACNNNNNNGTC 1 cut(s) 743
DriI GACNNNNNGTC 1 cut(s) 976
DseDI GACNNNNNNGTC 1 cut(s) 743
Eam1104I CTCTTC 1 cut(s) 47
Eam1105I GACNNNNNGTC 1 cut(s) 976
EarI CTCTTC 1 cut(s) 47
EciI GGCGGA 3 cut(s) 142, 841, 1037
Eco130I CCWWGG 1 cut(s) 1110
Eco24I GRGCYC 1 cut(s) 1236
Eco47I GGWCC 2 cut(s) 960, 1080
Eco88I CYCGRG 1 cut(s) 1056
Eco91I GGTNACC 1 cut(s) 1201
EcoO65I GGTNACC 1 cut(s) 1201
EcoRII CCWGG 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 1110
EcoT38I GRGCYC 1 cut(s) 1236
EgeI GGCGCC 3 cut(s) 244, 541, 841
EheI GGCGCC 3 cut(s) 244, 541, 841
ErhI CCWWGG 1 cut(s) 1110
FaeI CATG 3 cut(s) 301, 794, 997
FaiI YATR 5 cut(s) 299, 345, 792, 995, 1070
FalI AAGNNNNNCTT 2 cut(s) 1082, 1114
FaqI GGGAC 4 cut(s) 203, 553, 578, 973
FatI CATG 3 cut(s) 297, 790, 993
FauI CCCGC 8 cut(s) 173, 239, 347, 477, 646, 731, 950, 1243
FblI GTMKAC 1 cut(s) 980
FokI GGATG 4 cut(s) 55, 374, 386, 509
FriOI GRGCYC 1 cut(s) 1236
FspBI CTAG 1 cut(s) 716
GlaI GCGC 7 cut(s) 169, 244, 454, 541, 841, 1038, 1293
GsuI CTGGAG 1 cut(s) 337
HaeII RGCGCY 3 cut(s) 246, 543, 843
HaeIII GGCC 7 cut(s) 468, 666, 693, 944, 1003, 1061, 1234
HgaI GACGC 3 cut(s) 349, 669, 796
HhaI GCGC 7 cut(s) 170, 245, 455, 542, 842, 1039, 1294
Hin1I GRCGYC 4 cut(s) 243, 540, 807, 840
Hin1II CATG 3 cut(s) 301, 794, 997
Hin6I GCGC 7 cut(s) 168, 243, 453, 540, 840, 1037, 1292
HinP1I GCGC 7 cut(s) 168, 243, 453, 540, 840, 1037, 1292
HincII GTYRAC 1 cut(s) 981
HindII GTYRAC 1 cut(s) 981
HinfI GANTC 7 cut(s) 7, 426, 566, 645, 671, 729, 971
HphI GGTGA 2 cut(s) 1057, 1195
Hpy166II GTNNAC 5 cut(s) 970, 981, 987, 1053, 1126
Hpy188I TCNGA 5 cut(s) 121, 565, 632, 659, 784
Hpy188III TCNNGA 5 cut(s) 130, 423, 625, 826, 898
Hpy8I GTNNAC 5 cut(s) 970, 981, 987, 1053, 1126
HpyAV CCTTC 2 cut(s) 301, 406
HpyCH4III ACNGT 3 cut(s) 592, 800, 1123
HpyCH4IV ACGT 6 cut(s) 515, 519, 909, 983, 989, 1275
HpyCH4V TGCA 2 cut(s) 333, 438
HpyF10VI GCNNNNNNNGC 9 cut(s) 240, 339, 348, 354, 357, 647, 1034, 1169, 1231
HpyF3I CTNAG 3 cut(s) 118, 137, 260
HpySE526I ACGT 6 cut(s) 515, 519, 909, 983, 989, 1275
Hsp92I GRCGYC 4 cut(s) 243, 540, 807, 840
Hsp92II CATG 3 cut(s) 301, 794, 997
HspAI GCGC 7 cut(s) 168, 243, 453, 540, 840, 1037, 1292
KasI GGCGCC 3 cut(s) 242, 539, 839
KspI CCGCGG 1 cut(s) 235
Kzo9I GATC 2 cut(s) 121, 627
LmnI GCTCC 4 cut(s) 356, 539, 779, 1135
Lsp1109I GCAGC 8 cut(s) 321, 324, 335, 344, 422, 599, 762, 1273
MaeI CTAG 1 cut(s) 716
MaeII ACGT 6 cut(s) 515, 519, 909, 983, 989, 1275
MaeIII GTNAC 1 cut(s) 1201
MalI GATC 2 cut(s) 123, 629
MboI GATC 2 cut(s) 121, 627
MboII GAAGA 3 cut(s) 34, 355, 1177
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 1236
MluCI AATT 4 cut(s) 701, 1148, 1186, 1267
Mly113I GGCGCC 3 cut(s) 243, 540, 840
MlyI GAGTC 2 cut(s) 575, 965
MmeI TCCRAC 4 cut(s) 222, 655, 807, 870
MslI CAYNNNNRTG 1 cut(s) 992
MspA1I CMGCKG 4 cut(s) 234, 357, 771, 1209
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 1 cut(s) 457
MvnI CGCG 6 cut(s) 168, 234, 663, 861, 1034, 1238
MwoI GCNNNNNNNGC 9 cut(s) 240, 339, 348, 354, 357, 647, 1034, 1169, 1231
NarI GGCGCC 3 cut(s) 243, 540, 840
NciI CCSGG 9 cut(s) 652, 668, 837, 939, 976, 1057, 1058, 1084, 1133
NdeII GATC 2 cut(s) 121, 627
NlaIII CATG 3 cut(s) 301, 794, 997
NmeAIII GCCGAG 1 cut(s) 1087
NmuCI GTSAC 1 cut(s) 1201
PcsI WCGNNNNNNNCGW 4 cut(s) 741, 792, 986, 1281
PctI GAATGC 1 cut(s) 62
PfeI GAWTC 4 cut(s) 426, 645, 671, 729
Pfl23II CGTACG 1 cut(s) 516
PfoI TCCNGGA 1 cut(s) 1131
PleI GAGTC 2 cut(s) 574, 965
PluTI GGCGCC 3 cut(s) 246, 543, 843
PpsI GAGTC 2 cut(s) 574, 965
PshAI GACNNNNGTC 1 cut(s) 518
Psp6I CCWGG 1 cut(s) 455
PspEI GGTNACC 1 cut(s) 1201
PspGI CCWGG 1 cut(s) 455
PspLI CGTACG 1 cut(s) 516
PspOMI GGGCCC 1 cut(s) 1232
PspPI GGNCC 9 cut(s) 467, 665, 691, 942, 960, 1059, 1080, 1232, 1233
PsuI RGATCY 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 357
RsaI GTAC 6 cut(s) 518, 530, 866, 929, 1052, 1283
RsaNI GTAC 6 cut(s) 517, 529, 865, 928, 1051, 1282
RseI CAYNNNNRTG 1 cut(s) 992
Rsr2I CGGWCCG 1 cut(s) 1080
RsrII CGGWCCG 1 cut(s) 1080
SacII CCGCGG 1 cut(s) 235
SalI GTCGAC 1 cut(s) 979
Sau3AI GATC 2 cut(s) 121, 627
Sau96I GGNCC 9 cut(s) 467, 665, 691, 942, 960, 1059, 1080, 1232, 1233
ScaI AGTACT 2 cut(s) 866, 929
SchI GAGTC 2 cut(s) 575, 965
SduI GDGCHC 1 cut(s) 1236
SfcI CTRYAG 2 cut(s) 343, 766
SfoI GGCGCC 3 cut(s) 244, 541, 841
Sfr303I CCGCGG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 235
SinI GGWCC 2 cut(s) 960, 1080
SmaI CCCGGG 1 cut(s) 1058
SmiMI CAYNNNNRTG 1 cut(s) 992
Sse9I AATT 4 cut(s) 701, 1148, 1186, 1267
SspDI GGCGCC 3 cut(s) 242, 539, 839
SspMI CTAG 1 cut(s) 716
StyI CCWWGG 1 cut(s) 1110
TaaI ACNGT 3 cut(s) 592, 800, 1123
TaiI ACGT 6 cut(s) 518, 522, 912, 986, 992, 1278
TaqI TCGA 6 cut(s) 573, 626, 732, 980, 1011, 1278
TasI AATT 4 cut(s) 701, 1148, 1186, 1267
TatI WGTACW 3 cut(s) 864, 927, 1050
TauI GCSGC 8 cut(s) 237, 277, 280, 666, 774, 1037, 1212, 1215
TfiI GAWTC 4 cut(s) 426, 645, 671, 729
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 1 cut(s) 1201
TseI GCWGC 8 cut(s) 309, 312, 348, 357, 435, 612, 750, 1261
Tsp45I GTSAC 1 cut(s) 1201
TspDTI ATGAA 2 cut(s) 388, 982
TspMI CCCGGG 1 cut(s) 1056
TspRI CASTG 1 cut(s) 805
VpaK11BI GGWCC 2 cut(s) 960, 1080
XapI RAATTY 2 cut(s) 701, 1267
XcmI CCANNNNNNNNNTGG 3 cut(s) 295, 453, 797
XmaI CCCGGG 1 cut(s) 1056
XmiI GTMKAC 1 cut(s) 980
XspI CTAG 1 cut(s) 716
ZrmI AGTACT 2 cut(s) 866, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.