pycom12g03260

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015999
Physical Location & Seq
Forward (+)
2954409 .. 2955938
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g03260.1

Sequence Viewer

Length: 1530 bp
ATGTGCAACAAAAGGGCAGTTTGTGTTGCCCAAATCGAACTTGATTCTCCTCTTTCTACTTGCTTTCACTATAAAGAAGCTCACCAAAACCAAACCCTCGGAAAAGTACCAACTCTTGCTCCAATGGCAACTCCCACCTCCCTCCTCCTCTCTCTCACCACTCTCCTCTCTCTCCTCCTCCCTTCTCTCTCATCCAAACTCACCATCCCTCTTTCCCCATTCCCCAAATCACCGTCCACCGATCTACTCCAATCCCTCAACTTCCATGCCACCGCCTCCGTCTCCCGAGCCCGCCACATTAAAAACCCCAAAAAATCCGCCCACCCTTCTCCCTCCACCCAAGTCCCCCTCTTCCCCCACAGCTACGGCGGCTACTCCGTCTCCCTCCGCTTCGGCACGCCGCCGCAAACCTCCCCTTTCATCATGGACACCGGCAGCAGCCTCGTTTGGTTCCCCTGCACCAAACGCTACACCTGCTCCAAATGCCAGTTCCCAAACATCGACCCCTCAAAAATTCCAGCTTTCATCCCCAAACTCTCCTCCTCCTCCAAAATCGTCGGCTGCAAAAACCCCAAATGCGCTTGGATTTTCGGCCCCGAAGTCCAATCCAAATGCCAGAATTGCAACGACCCAACTCCCCAAAACTGCTCCCAAGCCTGCCCAACGTACATAGTCCAGTACGGTTCGGGCACAACAGCTGGGATTTTACTTTCCGAGTCACTAAATTTTCCCGAGAAAATTGTCCCTGATTTTCTCGTCGGCTGCTCCTTCCTCTCCATCCGCCAGCCCGCCGGAATCGCCGGATTCGGCCGCGGACCGCAATCCCTTCCTGCCCAAATGGGGCTTTCCAAATTCTCTTACTGCTTAGTCTCCCACAAATTCGACGACACCCCGCAAAGCAGCGACCTTGTTCTGTATACTGGCACCATTTCTTCAGCCCAAAAAAACGAAACGAAAGCTCAGAGCTCGATCTACACGCCGTTTCAGAAAAACCCAGGTTCTACAAACTCTGCATTTCGCGAGTACTACTATATCATGCTCAGAAAAATCATCGTGGGCAAGAAGCACGTCAATATTCCGTACAAGTTTCTCGTCCCCGGAGCCCACGGCAATGGCGGGACGATCGTGGATTCCCGATCGACCTTCACGTTCATGGAGAAGCCTGTTTTCGAAGCCGTTGCGAAAGAGTTTGAGTTACAGATGGCGAATTACACGAGAGCTAAGGACTTGGAAAACGGGACGGGATTGAGTCCCTGTTTTGATATTTCAAGGGAGGAGAAATTGGAGTTTCCGGAATTGGTTTTCCAGTTCAAAGGAGGTGCTAAGATGGAGCTGCCATTGACGAACTATTTCTCGCTGGTTTCGAGCTCCGGCGTTGTGTGCTTGACGATTGTTACTGATGGGGCTGTTGGTCCAGGGGGCAATGGTGGACCTGCGATTATTTTGGGGAACTATCAGCAGCAGAATTTCTTCGTGGAGTATGATTTGGAGCGTGAGAGGTTTGGGTTCCGGAAACAGAGTTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

510

Amino Acids

55.67

Weight (kDa)

9.04

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 125 - 294 4e-28 Xylanase inhibitor N-terminal
TAXi_C PF14541 343 - 504 3.1e-32 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52500
fragaria_vesca FvH4_6g20050
malus_domestica MD12G1037600.v1.1 MD14G1036600.v1.1
prunus_persica Prupe.7G097600_v2.0.a1
pyrus_communis pycom12g03260
rosa_chinensis RchiOBHm_Chr3g0475521
rosa_laevigata RLG00000023836
rosa_roxburghii Rroxscaffold_6G00406210
rosa_rugosa Rorug03G0150100
rosa_samantha Rh3AG199700 Rh3BG229700 Rh3CG224000 Rh3DG225000
rosa_wichuraiana Rw3G018100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 482
Acc36I ACCTGC 2 cut(s) 482, 1441
AccB1I GGYRCC 1 cut(s) 923
AccI GTMKAC 1 cut(s) 917
AccII CGCG 2 cut(s) 813, 1020
AccIII TCCGGA 2 cut(s) 1291, 1509
AcoI YGGCCR 1 cut(s) 808
AcsI RAATTY 5 cut(s) 513, 724, 851, 878, 1465
AcuI CTGAAG 1 cut(s) 918
AfaI GTAC 5 cut(s) 108, 668, 680, 1025, 1082
AfiI CCNNNNNNNGG 1 cut(s) 840
AgsI TTSAA 2 cut(s) 1269, 1312
AjiI CACGTC 1 cut(s) 1069
AjnI CCWGG 2 cut(s) 994, 1414
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AluBI AGCT 9 cut(s) 80, 363, 521, 698, 959, 966, 1220, 1333, 1368
AluI AGCT 9 cut(s) 80, 363, 521, 698, 959, 966, 1220, 1333, 1368
Alw21I GWGCWC 2 cut(s) 968, 1370
Alw26I GTCTC 3 cut(s) 286, 385, 874
Ama87I CYCGRG 2 cut(s) 285, 731
Aor13HI TCCGGA 2 cut(s) 1291, 1509
AoxI GGCC 2 cut(s) 592, 808
ApeKI GCWGC 7 cut(s) 435, 438, 561, 762, 900, 1333, 1459
ApoI RAATTY 5 cut(s) 513, 724, 851, 878, 1465
Asp700I GAANNNNTTC 2 cut(s) 1349, 1469
AspLEI GCGC 1 cut(s) 581
AspS9I GGNCC 4 cut(s) 593, 815, 1412, 1430
AsuC2I CCSGG 1 cut(s) 1098
AsuHPI GGTGA 4 cut(s) 74, 148, 193, 222
AsuII TTCGAA 1 cut(s) 1170
AvaI CYCGRG 2 cut(s) 285, 731
AvaII GGWCC 3 cut(s) 815, 1412, 1430
BaeGI GKGCMC 1 cut(s) 692
BanI GGYRCC 1 cut(s) 923
BanII GRGCYC 4 cut(s) 292, 968, 1105, 1370
BauI CACGAG 1 cut(s) 1213
Bbv12I GWGCWC 2 cut(s) 968, 1370
BbvI GCAGC 7 cut(s) 447, 450, 548, 749, 912, 1320, 1471
BccI CCATC 5 cut(s) 212, 785, 1195, 1321, 1394
BceAI ACGGC 4 cut(s) 382, 964, 1123, 1160
BcgI CGANNNNNNTGC 4 cut(s) 424, 458, 1160, 1194
BciT130I CCWGG 2 cut(s) 996, 1416
BcnI CCSGG 1 cut(s) 1098
BcoDI GTCTC 3 cut(s) 286, 385, 874
BfuAI ACCTGC 2 cut(s) 482, 1441
BmcAI AGTACT 1 cut(s) 1025
Bme1390I CCNGG 3 cut(s) 996, 1098, 1416
Bme18I GGWCC 3 cut(s) 815, 1412, 1430
BmeT110I CYCGRG 2 cut(s) 285, 731
BmgBI CACGTC 1 cut(s) 1069
BmgT120I GGNCC 4 cut(s) 593, 815, 1412, 1430
BmiI GGNNCC 5 cut(s) 452, 595, 925, 1102, 1508
BmrFI CCNGG 3 cut(s) 996, 1098, 1416
Bpu10I CCTNAGC 1 cut(s) 1221
Bpu14I TTCGAA 1 cut(s) 1170
BpuMI CCSGG 1 cut(s) 1098
BsaJI CCNNGG 6 cut(s) 97, 811, 994, 1096, 1105, 1415
BsaWI WCCGGW 2 cut(s) 1291, 1509
BsaXI ACNNNNNCTCC 6 cut(s) 103, 133, 365, 395, 461, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 840
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 4 cut(s) 487, 676, 925, 1306
Bse3DI GCAATG 2 cut(s) 1117, 1429
BseAI TCCGGA 2 cut(s) 1291, 1509
BseBI CCWGG 2 cut(s) 996, 1416
BseDI CCNNGG 6 cut(s) 97, 811, 994, 1096, 1105, 1415
BseGI GGATG 4 cut(s) 191, 204, 525, 777
BseLI CCNNNNNNNGG 1 cut(s) 840
BseMI GCAATG 2 cut(s) 1117, 1429
BseMII CTCAG 2 cut(s) 974, 1054
BseNI ACTGG 4 cut(s) 487, 676, 925, 1306
BseSI GKGCMC 1 cut(s) 692
BseX3I CGGCCG 1 cut(s) 808
BseXI GCAGC 7 cut(s) 447, 450, 548, 749, 912, 1320, 1471
BseYI CCCAGC 1 cut(s) 698
BsgI GTGCAG 1 cut(s) 442
Bsh1236I CGCG 2 cut(s) 813, 1020
Bsh1285I CGRYCG 3 cut(s) 811, 1125, 1139
BshFI GGCC 2 cut(s) 594, 810
BshNI GGYRCC 1 cut(s) 923
BsiEI CGRYCG 3 cut(s) 811, 1125, 1139
BsiHKAI GWGCWC 2 cut(s) 968, 1370
BsiHKCI CYCGRG 2 cut(s) 285, 731
BsiSI CCGG 7 cut(s) 432, 792, 801, 1098, 1292, 1371, 1510
BslFI GGGAC 6 cut(s) 329, 728, 1079, 1132, 1236, 1252
BslI CCNNNNNNNGG 1 cut(s) 840
BsmAI GTCTC 3 cut(s) 286, 385, 874
BsmBI CGTCTC 2 cut(s) 286, 385
BsmFI GGGAC 6 cut(s) 329, 728, 1079, 1132, 1236, 1252
BsnI GGCC 2 cut(s) 594, 810
BsoBI CYCGRG 2 cut(s) 285, 731
Bsp119I TTCGAA 1 cut(s) 1170
Bsp1286I GDGCHC 5 cut(s) 292, 692, 968, 1105, 1370
Bsp13I TCCGGA 2 cut(s) 1291, 1509
Bsp143I GATC 4 cut(s) 241, 969, 1122, 1136
Bsp68I TCGCGA 1 cut(s) 1020
BspANI GGCC 2 cut(s) 594, 810
BspCNI CTCAG 2 cut(s) 973, 1053
BspEI TCCGGA 2 cut(s) 1291, 1509
BspFNI CGCG 2 cut(s) 813, 1020
BspLI GGNNCC 5 cut(s) 452, 595, 925, 1102, 1508
BspMI ACCTGC 2 cut(s) 482, 1441
BspT104I TTCGAA 1 cut(s) 1170
BspT107I GGYRCC 1 cut(s) 923
BsrDI GCAATG 2 cut(s) 1117, 1429
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 4 cut(s) 487, 676, 925, 1306
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 6 cut(s) 97, 811, 994, 1096, 1105, 1415
BssMI GATC 4 cut(s) 241, 969, 1122, 1136
BssNAI GTATAC 1 cut(s) 918
BssSI CACGAG 1 cut(s) 1213
Bst1107I GTATAC 1 cut(s) 918
Bst2BI CACGAG 1 cut(s) 1213
Bst2UI CCWGG 2 cut(s) 996, 1416
Bst4CI ACNGT 2 cut(s) 234, 683
Bst6I CTCTTC 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 1170
BstC8I GCNNGC 5 cut(s) 292, 398, 658, 785, 789
BstDEI CTNAG 5 cut(s) 865, 960, 1040, 1221, 1323
BstDSI CCRYGG 2 cut(s) 811, 1105
BstF5I GGATG 4 cut(s) 191, 204, 525, 777
BstFNI CGCG 2 cut(s) 813, 1020
BstHHI GCGC 1 cut(s) 581
BstKTI GATC 4 cut(s) 244, 972, 1125, 1139
BstMAI GTCTC 3 cut(s) 286, 385, 874
BstMBI GATC 4 cut(s) 241, 969, 1122, 1136
BstMCI CGRYCG 3 cut(s) 811, 1125, 1139
BstMWI GCNNNNNNNGC 8 cut(s) 125, 369, 465, 474, 483, 621, 797, 1380
BstNI CCWGG 2 cut(s) 996, 1416
BstSCI CCNGG 3 cut(s) 994, 1096, 1414
BstSLI GKGCMC 1 cut(s) 692
BstUI CGCG 2 cut(s) 813, 1020
BstV1I GCAGC 7 cut(s) 447, 450, 548, 749, 912, 1320, 1471
BstXI CCANNNNNNTGG 1 cut(s) 1112
BstZ17I GTATAC 1 cut(s) 918
BstZI CGGCCG 1 cut(s) 808
BsuRI GGCC 2 cut(s) 594, 810
BtgI CCRYGG 2 cut(s) 811, 1105
BtrI CACGTC 1 cut(s) 1069
BtsCI GGATG 4 cut(s) 191, 204, 525, 777
BtuMI TCGCGA 1 cut(s) 1020
BveI ACCTGC 2 cut(s) 482, 1441
Cac8I GCNNGC 5 cut(s) 292, 398, 658, 785, 789
CfoI GCGC 1 cut(s) 581
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 4 cut(s) 593, 815, 1412, 1430
Cfr42I CCGCGG 1 cut(s) 814
CpoI CGGWCCG 1 cut(s) 815
Csp6I GTAC 5 cut(s) 107, 667, 679, 1024, 1081
CspI CGGWCCG 1 cut(s) 815
CviAII CATG 4 cut(s) 266, 424, 1036, 1153
CviQI GTAC 5 cut(s) 107, 667, 679, 1024, 1081
DdeI CTNAG 5 cut(s) 865, 960, 1040, 1221, 1323
DpnI GATC 4 cut(s) 243, 971, 1124, 1138
DpnII GATC 4 cut(s) 241, 969, 1122, 1136
EaeI YGGCCR 1 cut(s) 808
EagI CGGCCG 1 cut(s) 808
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
EciI GGCGGA 2 cut(s) 307, 770
Ecl136II GAGCTC 2 cut(s) 966, 1368
EclXI CGGCCG 1 cut(s) 808
Eco24I GRGCYC 4 cut(s) 292, 968, 1105, 1370
Eco47I GGWCC 3 cut(s) 815, 1412, 1430
Eco52I CGGCCG 1 cut(s) 808
Eco53kI GAGCTC 2 cut(s) 966, 1368
Eco57I CTGAAG 1 cut(s) 918
Eco88I CYCGRG 2 cut(s) 285, 731
EcoICRI GAGCTC 2 cut(s) 966, 1368
EcoRII CCWGG 2 cut(s) 994, 1414
EcoT38I GRGCYC 4 cut(s) 292, 968, 1105, 1370
Esp3I CGTCTC 2 cut(s) 286, 385
FaeI CATG 4 cut(s) 269, 427, 1039, 1156
FaiI YATR 9 cut(s) 72, 267, 425, 671, 918, 1032, 1037, 1154, 1482
FaqI GGGAC 6 cut(s) 329, 728, 1079, 1132, 1236, 1252
FatI CATG 4 cut(s) 265, 423, 1035, 1152
FauI CCCGC 4 cut(s) 299, 796, 900, 1109
FblI GTMKAC 1 cut(s) 917
FokI GGATG 4 cut(s) 178, 191, 512, 764
FriOI GRGCYC 4 cut(s) 292, 968, 1105, 1370
GlaI GCGC 1 cut(s) 580
GsaI CCCAGC 1 cut(s) 702
HaeIII GGCC 2 cut(s) 594, 810
HapII CCGG 7 cut(s) 432, 792, 801, 1098, 1292, 1371, 1510
HhaI GCGC 1 cut(s) 581
Hin1II CATG 4 cut(s) 269, 427, 1039, 1156
Hin6I GCGC 1 cut(s) 579
HinP1I GCGC 1 cut(s) 579
HinfI GANTC 6 cut(s) 44, 716, 795, 804, 1130, 1249
HpaII CCGG 7 cut(s) 432, 792, 801, 1098, 1292, 1371, 1510
HphI GGTGA 4 cut(s) 74, 148, 193, 222
Hpy166II GTNNAC 3 cut(s) 237, 918, 1430
Hpy188I TCNGA 5 cut(s) 101, 715, 963, 987, 1043
Hpy188III TCNNGA 6 cut(s) 285, 731, 1019, 1134, 1292, 1510
Hpy8I GTNNAC 3 cut(s) 237, 918, 1430
Hpy99I CGWCG 3 cut(s) 560, 761, 887
HpyAV CCTTC 5 cut(s) 192, 336, 778, 836, 1153
HpyCH4III ACNGT 2 cut(s) 234, 683
HpyCH4IV ACGT 3 cut(s) 665, 1068, 1148
HpyCH4V TGCA 5 cut(s) 6, 459, 564, 624, 1013
HpyF10VI GCNNNNNNNGC 8 cut(s) 125, 369, 465, 474, 483, 621, 797, 1380
HpyF3I CTNAG 5 cut(s) 865, 960, 1040, 1221, 1323
HpySE526I ACGT 3 cut(s) 665, 1068, 1148
Hsp92II CATG 4 cut(s) 269, 427, 1039, 1156
HspAI GCGC 1 cut(s) 579
Kpn2I TCCGGA 2 cut(s) 1291, 1509
KspI CCGCGG 1 cut(s) 814
Kzo9I GATC 4 cut(s) 241, 969, 1122, 1136
LmnI GCTCC 8 cut(s) 124, 482, 653, 770, 1100, 1330, 1373, 1489
Lsp1109I GCAGC 7 cut(s) 447, 450, 548, 749, 912, 1320, 1471
MaeII ACGT 3 cut(s) 665, 1068, 1148
MaeIII GTNAC 3 cut(s) 717, 1194, 1393
MalI GATC 4 cut(s) 243, 971, 1124, 1138
MboI GATC 4 cut(s) 241, 969, 1122, 1136
MboII GAAGA 3 cut(s) 343, 924, 1462
MhlI GDGCHC 5 cut(s) 292, 692, 968, 1105, 1370
MlyI GAGTC 2 cut(s) 725, 1258
MroI TCCGGA 2 cut(s) 1291, 1509
MroXI GAANNNNTTC 2 cut(s) 1349, 1469
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 2 cut(s) 1110, 1151
MspA1I CMGCKG 2 cut(s) 698, 813
MspI CCGG 7 cut(s) 432, 792, 801, 1098, 1292, 1371, 1510
MspR9I CCNGG 3 cut(s) 996, 1098, 1416
MvaI CCWGG 2 cut(s) 996, 1416
MvnI CGCG 2 cut(s) 813, 1020
MwoI GCNNNNNNNGC 8 cut(s) 125, 369, 465, 474, 483, 621, 797, 1380
NciI CCSGG 1 cut(s) 1098
NdeII GATC 4 cut(s) 241, 969, 1122, 1136
NlaIII CATG 4 cut(s) 269, 427, 1039, 1156
NlaIV GGNNCC 5 cut(s) 452, 595, 925, 1102, 1508
NmuCI GTSAC 1 cut(s) 717
NruI TCGCGA 1 cut(s) 1020
NspV TTCGAA 1 cut(s) 1170
PaqCI CACCTGC 1 cut(s) 482
PcsI WCGNNNNNNNCGW 2 cut(s) 1145, 1361
PdmI GAANNNNTTC 2 cut(s) 1349, 1469
PfeI GAWTC 4 cut(s) 44, 795, 804, 1130
Ple19I CGATCG 2 cut(s) 1125, 1139
PleI GAGTC 2 cut(s) 724, 1257
PpsI GAGTC 2 cut(s) 724, 1257
Psp124BI GAGCTC 2 cut(s) 968, 1370
Psp6I CCWGG 2 cut(s) 994, 1414
PspFI CCCAGC 1 cut(s) 698
PspGI CCWGG 2 cut(s) 994, 1414
PspN4I GGNNCC 5 cut(s) 452, 595, 925, 1102, 1508
PspPI GGNCC 4 cut(s) 593, 815, 1412, 1430
PvuI CGATCG 2 cut(s) 1125, 1139
PvuII CAGCTG 1 cut(s) 698
RruI TCGCGA 1 cut(s) 1020
RsaI GTAC 5 cut(s) 108, 668, 680, 1025, 1082
RsaNI GTAC 5 cut(s) 107, 667, 679, 1024, 1081
RseI CAYNNNNRTG 2 cut(s) 1110, 1151
Rsr2I CGGWCCG 1 cut(s) 815
RsrII CGGWCCG 1 cut(s) 815
SacI GAGCTC 2 cut(s) 968, 1370
SacII CCGCGG 1 cut(s) 814
SaqAI TTAA 1 cut(s) 300
Sau3AI GATC 4 cut(s) 241, 969, 1122, 1136
Sau96I GGNCC 4 cut(s) 593, 815, 1412, 1430
ScaI AGTACT 1 cut(s) 1025
SchI GAGTC 2 cut(s) 725, 1258
ScrFI CCNGG 3 cut(s) 996, 1098, 1416
SduI GDGCHC 5 cut(s) 292, 692, 968, 1105, 1370
Sfr303I CCGCGG 1 cut(s) 814
SfuI TTCGAA 1 cut(s) 1170
SgrBI CCGCGG 1 cut(s) 814
SinI GGWCC 3 cut(s) 815, 1412, 1430
SmiMI CAYNNNNRTG 2 cut(s) 1110, 1151
SspI AATATT 1 cut(s) 1075
SstI GAGCTC 2 cut(s) 968, 1370
StyD4I CCNGG 3 cut(s) 994, 1096, 1414
TaaI ACNGT 2 cut(s) 234, 683
TaiI ACGT 3 cut(s) 668, 1071, 1151
TaqI TCGA 7 cut(s) 36, 501, 882, 968, 1139, 1170, 1364
TatI WGTACW 1 cut(s) 1023
TauI GCSGC 4 cut(s) 372, 403, 406, 813
TfiI GAWTC 4 cut(s) 44, 795, 804, 1130
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TseFI GTSAC 1 cut(s) 717
TseI GCWGC 7 cut(s) 435, 438, 561, 762, 900, 1333, 1459
Tsp45I GTSAC 1 cut(s) 717
TspDTI ATGAA 3 cut(s) 409, 514, 1141
TspGWI ACGGA 3 cut(s) 268, 367, 1068
VpaK11BI GGWCC 3 cut(s) 815, 1412, 1430
XapI RAATTY 5 cut(s) 513, 724, 851, 878, 1465
XmiI GTMKAC 1 cut(s) 917
XmnI GAANNNNTTC 2 cut(s) 1349, 1469
ZrmI AGTACT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.