AT3G52940

Ergosterol biosynthesis ERG4/ERG24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011259
Physical Location & Seq
Reverse (-)
19630077 .. 19633328
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G52940.2

Sequence Viewer

Length: 1110 bp
ATGCTGCTAGATATGGATCTCGGTGTTCTTCTTCCATCATTGCAATCTGTTTATGTGCTGGTGTTTTACTTCGTTTACTTGGCCGTTGCCGGAGAAATTCTCCCCGGGAAAGTTATTCGCGGCGTCCTTTTATCAGATGGCTCTCAACTTCGTTACCGATGCAATGGTCTATTGGCACTAATATTGTTGGTAGCTATTTTGGGAATCTGTGCAAAACTTGGCATTGTATCACCTCTTGTGGTTGCGGATAGAGGACTTGAGTTACTCTCAGCTACTTTTATTTTCTGTGTTTTGGTGACATTAGCATTGTATGTTACTGGGCGAAGTTCCTCGAATAAGGGTTCTTCCCTAAAGCCTCATGTCTCAGGAAATCTTGTACATGACTGGTGGTTTGGAATACAGCTGAATCCTCAGTTTATGAGCATTGATCTCAAGTTTTTCTTTGTCAGAGCCGGGATGATGGGATGGCTGCTTATCAATCTCTCTATTCTGGCAAAAAGTGTGCAGGATGGTTCCTTGAGTCAGTCGATGATTCTTTACCAGATCTTCTGTGCGTTATATATATTGGACTACTTTGTTCATGAAGAATACATGACCTCTACGTGGGACATAATTGCAGAGAGACTAGGCTTCATGCTAGTGTTTGGAGATCTCCTGTGGATTCCTTTCACTTTTAGCATTCAGGGCTGGTGGCTTTTGCACAACAAAGTAGAACTAACAGTTCCTGCGATTGTAGTCAATTGCCTTGTCTTCTTGATAGGGTACATGGTTTTTCGAGGAGCTAACAAACAAAAACATATCTTTAAGAAGAACCCAAAAACACCAATATGGGGCAAGCCTCCAGTGGTAGTTGGTGGAAAGTTACTGGTTTCAGGCTATTGGGGAATTGCAAGGCACTGTAATTACCTTGGCGACTTGATGCTTGCTCTGTCCTTCAGTTTGCCATGTGGAATAAGTTCTCCGGTTCCATATTTCTACCCGATATACCTTCTGATACTATTGATATGGAGAGAACGAAGAGACGAGGTTCGATGTGCAGAGAAGTACAAGGAGATATGGGCAGAGTATCTTAGACTTGTCCCCTGGAGAATACTTCCTTATGTTTATTAG

Protein Analysis

369

Amino Acids

41.91

Weight (kDa)

8.98

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERG4_ERG24 PF01222 15 - 232 4.1e-47 Ergosterol biosynthesis ERG4/ERG24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52940
fragaria_vesca FvH4_6g17830 FvH4_6g17830
malus_domestica MD12G1017500.v1.1 MD14G1015200.v1.1
prunus_persica Prupe.7G118200_v2.0.a1 Prupe.7G118200_v2.0.a1 Prupe.8G104900_v2.0.a1
pyrus_communis pycom12g01410 pycom14g01270
rosa_chinensis RchiOBHm_Chr3g0472081
rosa_laevigata RLG00000024106
rosa_multiflora Rmu_sc0002532.1_g000014
rosa_roxburghii Rroxscaffold_6G00409260
rosa_rugosa Rorug03G0122000
rosa_samantha Rh3AG173800 Rh3BG199200 Rh3CG197700 Rh3DG175300
rosa_wichuraiana Rw3G016680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 120
AciI CCGC 2 cut(s) 120, 245
AclWI GGATC 1 cut(s) 24
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 96
AcuI CTGAAG 1 cut(s) 919
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 3 cut(s) 378, 764, 1046
AfiI CCNNNNNNNGG 2 cut(s) 603, 830
AjnI CCWGG 1 cut(s) 1082
AluBI AGCT 4 cut(s) 194, 272, 403, 782
AluI AGCT 4 cut(s) 194, 272, 403, 782
Alw26I GTCTC 3 cut(s) 367, 616, 1014
AlwI GGATC 1 cut(s) 24
AlwNI CAGNNNCTG 1 cut(s) 725
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 2 cut(s) 4, 469
ApoI RAATTY 1 cut(s) 96
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
Asp700I GAANNNNTTC 1 cut(s) 955
AsuC2I CCSGG 3 cut(s) 105, 106, 454
AsuHPI GGTGA 2 cut(s) 222, 307
AvaI CYCGRG 1 cut(s) 104
BbsI GAAGAC 1 cut(s) 742
BbvI GCAGC 1 cut(s) 456
BccI CCATC 5 cut(s) 43, 131, 454, 459, 503
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 1084
BcnI CCSGG 3 cut(s) 105, 106, 454
BcoDI GTCTC 3 cut(s) 367, 616, 1014
BfaI CTAG 3 cut(s) 8, 626, 638
BglII AGATCT 2 cut(s) 543, 649
BisI GCNGC 3 cut(s) 5, 121, 470
BlsI GCNGC 3 cut(s) 6, 122, 471
Bme1390I CCNGG 4 cut(s) 105, 106, 454, 1084
BmeT110I CYCGRG 1 cut(s) 104
BmiI GGNNCC 2 cut(s) 514, 966
BmrFI CCNGG 4 cut(s) 105, 106, 454, 1084
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 2 cut(s) 149, 909
BmuI ACTGGG 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 742
BplI GAGNNNNNCTC 4 cut(s) 84, 116, 251, 283
BpmI CTGGAG 2 cut(s) 825, 1105
BpuEI CTTGAG 3 cut(s) 278, 416, 538
BpuMI CCSGG 3 cut(s) 105, 106, 454
BsaAI YACGTR 1 cut(s) 603
BsaBI GATNNNNATC 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 4 cut(s) 103, 104, 907, 1082
BsaWI WCCGGW 1 cut(s) 961
Bsc4I CCNNNNNNNGG 2 cut(s) 603, 830
Bse1I ACTGG 4 cut(s) 322, 389, 842, 870
Bse3DI GCAATG 2 cut(s) 38, 169
Bse8I GATNNNNATC 1 cut(s) 15
BseBI CCWGG 1 cut(s) 1084
BseDI CCNNGG 4 cut(s) 103, 104, 907, 1082
BseGI GGATG 3 cut(s) 462, 470, 514
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 2 cut(s) 603, 830
BseMI GCAATG 2 cut(s) 38, 169
BseMII CTCAG 3 cut(s) 282, 378, 425
BseNI ACTGG 4 cut(s) 322, 389, 842, 870
BseRI GAGGAG 1 cut(s) 792
BseXI GCAGC 1 cut(s) 456
BsgI GTGCAG 2 cut(s) 524, 1056
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 83
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 4 cut(s) 90, 105, 453, 962
BslFI GGGAC 2 cut(s) 620, 1064
BslI CCNNNNNNNGG 2 cut(s) 603, 830
BsmAI GTCTC 3 cut(s) 367, 616, 1014
BsmBI CGTCTC 1 cut(s) 1014
BsmFI GGGAC 2 cut(s) 620, 1064
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 1 cut(s) 83
BsoBI CYCGRG 1 cut(s) 104
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 4 cut(s) 16, 427, 543, 649
BspACI CCGC 2 cut(s) 120, 245
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 3 cut(s) 281, 377, 424
BspFNI CGCG 1 cut(s) 120
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 2 cut(s) 514, 966
BspPI GGATC 1 cut(s) 24
BsrDI GCAATG 2 cut(s) 38, 169
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 4 cut(s) 322, 389, 842, 870
BssECI CCNNGG 4 cut(s) 103, 104, 907, 1082
BssMI GATC 4 cut(s) 16, 427, 543, 649
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 907
Bst2UI CCWGG 1 cut(s) 1084
Bst4CI ACNGT 2 cut(s) 721, 899
Bst6I CTCTTC 1 cut(s) 1012
BstACI GRCGYC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 603
BstC8I GCNNGC 2 cut(s) 836, 924
BstDEI CTNAG 4 cut(s) 268, 364, 411, 1070
BstF5I GGATG 3 cut(s) 462, 470, 514
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 4 cut(s) 19, 430, 546, 652
BstMAI GTCTC 3 cut(s) 367, 616, 1014
BstMBI GATC 4 cut(s) 16, 427, 543, 649
BstMWI GCNNNNNNNGC 1 cut(s) 684
BstNI CCWGG 1 cut(s) 1084
BstSCI CCNGG 4 cut(s) 103, 104, 452, 1082
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 456
BstV2I GAAGAC 1 cut(s) 742
BstX2I RGATCY 3 cut(s) 16, 543, 649
BstYI RGATCY 3 cut(s) 16, 543, 649
BsuRI GGCC 1 cut(s) 83
BtsCI GGATG 3 cut(s) 462, 470, 514
BtsIMutI CAGTG 2 cut(s) 849, 895
Cac8I GCNNGC 2 cut(s) 836, 924
CaiI CAGNNNCTG 1 cut(s) 725
CciI TCATGA 1 cut(s) 580
Cfr9I CCCGGG 1 cut(s) 104
CseI GACGC 1 cut(s) 112
Csp6I GTAC 3 cut(s) 377, 763, 1045
CviAII CATG 7 cut(s) 359, 380, 581, 592, 634, 766, 945
CviQI GTAC 3 cut(s) 377, 763, 1045
DdeI CTNAG 4 cut(s) 268, 364, 411, 1070
DpnI GATC 4 cut(s) 18, 429, 545, 651
DpnII GATC 4 cut(s) 16, 427, 543, 649
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 1012
EarI CTCTTC 1 cut(s) 1012
Eco130I CCWWGG 1 cut(s) 907
Eco57I CTGAAG 1 cut(s) 919
Eco88I CYCGRG 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 1082
EcoT14I CCWWGG 1 cut(s) 907
ErhI CCWWGG 1 cut(s) 907
Esp3I CGTCTC 1 cut(s) 1014
FaeI CATG 7 cut(s) 362, 383, 584, 595, 637, 769, 948
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 2 cut(s) 620, 1064
FatI CATG 7 cut(s) 358, 379, 580, 591, 633, 765, 944
Fnu4HI GCNGC 3 cut(s) 5, 121, 470
FokI GGATG 3 cut(s) 469, 477, 521
Fsp4HI GCNGC 3 cut(s) 5, 121, 470
FspBI CTAG 3 cut(s) 8, 626, 638
GluI GCNGC 3 cut(s) 5, 121, 470
GsuI CTGGAG 2 cut(s) 825, 1105
HaeIII GGCC 1 cut(s) 83
HapII CCGG 4 cut(s) 90, 105, 453, 962
HgaI GACGC 1 cut(s) 112
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 7 cut(s) 362, 383, 584, 595, 637, 769, 948
HinfI GANTC 5 cut(s) 204, 406, 520, 532, 661
HpaII CCGG 4 cut(s) 90, 105, 453, 962
HphI GGTGA 2 cut(s) 222, 307
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 3 cut(s) 136, 449, 993
Hpy188III TCNNGA 3 cut(s) 366, 581, 754
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 2 cut(s) 943, 998
HpyCH4III ACNGT 2 cut(s) 721, 899
HpyCH4IV ACGT 1 cut(s) 602
HpyCH4V TGCA 8 cut(s) 43, 162, 212, 505, 617, 700, 890, 1037
HpyF10VI GCNNNNNNNGC 1 cut(s) 684
HpyF3I CTNAG 4 cut(s) 268, 364, 411, 1070
HpySE526I ACGT 1 cut(s) 602
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 7 cut(s) 362, 383, 584, 595, 637, 769, 948
Kzo9I GATC 4 cut(s) 16, 427, 543, 649
LmnI GCTCC 1 cut(s) 779
Lsp1109I GCAGC 1 cut(s) 456
LweI GCATC 2 cut(s) 149, 909
MaeI CTAG 3 cut(s) 8, 626, 638
MaeII ACGT 1 cut(s) 602
MaeIII GTNAC 5 cut(s) 152, 261, 295, 313, 861
MalI GATC 4 cut(s) 18, 429, 545, 651
MboI GATC 4 cut(s) 16, 427, 543, 649
MboII GAAGA 8 cut(s) 20, 23, 336, 538, 596, 742, 820, 1029
MfeI CAATTG 1 cut(s) 739
MflI RGATCY 3 cut(s) 16, 543, 649
MluCI AATT 5 cut(s) 96, 612, 739, 885, 901
MlyI GAGTC 1 cut(s) 529
MnlI CCTC 9 cut(s) 243, 245, 340, 366, 420, 607, 770, 849, 1018
MroXI GAANNNNTTC 1 cut(s) 955
MseI TTAA 1 cut(s) 804
MslI CAYNNNNRTG 2 cut(s) 638, 826
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 4 cut(s) 90, 105, 453, 962
MspR9I CCNGG 4 cut(s) 105, 106, 454, 1084
MunI CAATTG 1 cut(s) 739
Mva1269I GAATGC 1 cut(s) 678
MvaI CCWGG 1 cut(s) 1084
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 684
NciI CCSGG 3 cut(s) 105, 106, 454
NdeII GATC 4 cut(s) 16, 427, 543, 649
NlaIII CATG 7 cut(s) 362, 383, 584, 595, 637, 769, 948
NlaIV GGNNCC 2 cut(s) 514, 966
NmuCI GTSAC 1 cut(s) 295
PagI TCATGA 1 cut(s) 580
PctI GAATGC 1 cut(s) 678
PdmI GAANNNNTTC 1 cut(s) 955
PfeI GAWTC 4 cut(s) 204, 406, 532, 661
PkrI GCNGC 3 cut(s) 6, 122, 471
PleI GAGTC 1 cut(s) 528
PpsI GAGTC 1 cut(s) 528
Ppu21I YACGTR 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 1082
PspGI CCWGG 1 cut(s) 1082
PspN4I GGNNCC 2 cut(s) 514, 966
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 3 cut(s) 16, 543, 649
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 3 cut(s) 378, 764, 1046
RsaNI GTAC 3 cut(s) 377, 763, 1045
RseI CAYNNNNRTG 2 cut(s) 638, 826
SaqAI TTAA 1 cut(s) 804
SatI GCNGC 3 cut(s) 5, 121, 470
Sau3AI GATC 4 cut(s) 16, 427, 543, 649
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 4 cut(s) 105, 106, 454, 1084
SfaNI GCATC 2 cut(s) 149, 909
SmaI CCCGGG 1 cut(s) 106
SmiMI CAYNNNNRTG 2 cut(s) 638, 826
SmlI CTYRAG 3 cut(s) 257, 431, 517
SmoI CTYRAG 3 cut(s) 257, 431, 517
Sse9I AATT 5 cut(s) 96, 612, 739, 885, 901
SsiI CCGC 2 cut(s) 120, 245
SspI AATATT 1 cut(s) 183
SspMI CTAG 3 cut(s) 8, 626, 638
StyD4I CCNGG 4 cut(s) 103, 104, 452, 1082
StyI CCWWGG 1 cut(s) 907
TaaI ACNGT 2 cut(s) 721, 899
TaiI ACGT 1 cut(s) 605
TaqI TCGA 4 cut(s) 332, 527, 775, 1030
TasI AATT 5 cut(s) 96, 612, 739, 885, 901
TatI WGTACW 2 cut(s) 376, 1044
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 4 cut(s) 204, 406, 532, 661
Tru1I TTAA 1 cut(s) 804
Tru9I TTAA 1 cut(s) 804
TscAI CASTG 2 cut(s) 849, 902
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 2 cut(s) 4, 469
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 3 cut(s) 569, 597, 622
TspMI CCCGGG 1 cut(s) 104
TspRI CASTG 2 cut(s) 849, 902
XapI RAATTY 1 cut(s) 96
XmaI CCCGGG 1 cut(s) 104
XmnI GAANNNNTTC 1 cut(s) 955
XspI CTAG 3 cut(s) 8, 626, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.