Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0011259

Physical Location & Seq

Genomic Coordinates Forward (+)

1334762 .. 1340076

Gene Structure 5315 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1015200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGGATCTGGATCATCTTCTACACCTCCTAATTCCCTCATGGACCTCTGTTGCTATCCTGGCATTGTTCTTCACTTACTTGGCCATTGCTGGATCTTTACTGCCCGGAAAACTTGTCCCCGGAGCCACCCTACCGGATGGCTCTCGGCTTCATTATCGATGCAATGGTCTGCTATCACTTCTGTGGCTGGTTGGGCTTCTTGGGGTCGGCGCTAAGATGGATATCGTATCGCTTACTGCAATATCAGATAGAGGACTCGAGCTGTTGTCAACAACTTTTCTGTTTAGTGTTCTGGTCACAATGGTACTTTACGCTGCGGGCTGCAAGTCGACCAACCGGGGTTCTTCCCTGAAGCCTCATGTCACAGGGAACCTTCTTGAGGACTGGTGGTTTGGAATACAGCTTAATCCGCAGTTTATGGGCATTGACCTCAAATTTTTCTTTGTTAGAGCTGGAATGATGGGCTGGCTATTTATCAACCTTTCGGTTCTCGCCAGAAGTGTTCAAGATGGTACCTTGAGTCAATCAATGTTCCTCTTCCAGTTGTTTTGTGCGTTATACATCTTGGACTACTTTGTTCATGAGGAGTACATGACCTCCACATGGGACATAATCGCAGAGAGACTGGGCTTCATGCTGGTATTTGGAGATCTAGTCTGGATTCCGTTCACTTTCAGCATCCAGGGATGGTGGCTTCTGAGCAATAAGGTGGAGTTAGGAACAGCGGCTGTCGTAGCAAATTGCTTGACATTTCTGATTGGGTACCTTGTATTCAGAGGAGCCAACAAGCAAAAGCACGTCTTTAAGAAGAATCCCAAAGCACCCATATGGGGTAAGCCCTGTAGGGTCATTGGGGGAAAGTTGCTTGCTTCTGGTTATTGGGGAATTGCAAGACACTGTAATTACCTCGGGGACTTGTTACTTGCACTGTCTCTCAGCTTACCTTGCGGGATAAGTTCCCCAGTTCCATACTTCTATCCAATTTATCTTCTTATTCTGCTCATATGGAGAGAAAGACGAGACGAAGCTCGTTGTGCTGAGAAGTACAGAGAGATCTGGGCCGAATACTGCCGGCTTGTTCCATGGAGAATATTACCTTATGTTTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0012505 GO:0016020 GO:0016021 GO:0016491 GO:0016627 GO:0016628 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0036094 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048037 GO:0050613 GO:0050661 GO:0050662 GO:0055114 GO:0097159 GO:0098827 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

ERG4_ERG24

Protein Analysis

370

Amino Acids

41.75

Weight (kDa)

8.88

Isoelectric Point (pI)

26.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ERG4_ERG24	PF01222	10 - 369	1e-95	Ergosterol biosynthesis ERG4/ERG24 family
DUF1295	PF06966	207 - 314	1.5e-06	Protein of unknown function (DUF1295)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011259)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52940
fragaria_vesca	FvH4_6g17830 FvH4_6g17830
malus_domestica	MD12G1017500.v1.1 MD14G1015200.v1.1
prunus_persica	Prupe.7G118200_v2.0.a1 Prupe.7G118200_v2.0.a1 Prupe.8G104900_v2.0.a1
pyrus_communis	pycom12g01410 pycom14g01270
rosa_chinensis	RchiOBHm_Chr3g0472081
rosa_laevigata	RLG00000024106
rosa_multiflora	Rmu_sc0002532.1_g000014
rosa_roxburghii	Rroxscaffold_6G00409260
rosa_rugosa	Rorug03G0122000
rosa_samantha	Rh3AG173800 Rh3BG199200 Rh3CG197700 Rh3DG175300
rosa_wichuraiana	Rw3G016680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	512, 762
AccB1I	GGYRCC	2 cut(s)	512, 762
AccI	GTMKAC	1 cut(s)	329
AciI	CCGC	4 cut(s)	317, 410, 725, 948
AclWI	GGATC	3 cut(s)	12, 18, 100
AcoI	YGGCCR	1 cut(s)	81
AcsI	RAATTY	1 cut(s)	434
AcuI	CTGAAG	1 cut(s)	371
AfaI	GTAC	5 cut(s)	306, 514, 590, 764, 1046
AfiI	CCNNNNNNNGG	3 cut(s)	379, 603, 830
AgsI	TTSAA	1 cut(s)	506
AjiI	CACGTC	1 cut(s)	799
AjnI	CCWGG	2 cut(s)	57, 681
AleI	CACNNNNGTG	1 cut(s)	181
AluBI	AGCT	5 cut(s)	262, 403, 452, 939, 1028
AluI	AGCT	5 cut(s)	262, 403, 452, 939, 1028
Alw26I	GTCTC	3 cut(s)	616, 936, 1014
AlwI	GGATC	3 cut(s)	12, 18, 100
AlwNI	CAGNNNCTG	1 cut(s)	728
Ama87I	CYCGRG	2 cut(s)	257, 908
AoxI	GGCC	2 cut(s)	81, 1059
ApeKI	GCWGC	2 cut(s)	314, 321
ApoI	RAATTY	1 cut(s)	434
ArsI	GACNNNNNNTTYG	2 cut(s)	374, 406
Asp718I	GGTACC	2 cut(s)	512, 762
AspLEI	GCGC	1 cut(s)	212
AspS9I	GGNCC	2 cut(s)	42, 1059
AsuC2I	CCSGG	3 cut(s)	105, 120, 338
AvaI	CYCGRG	2 cut(s)	257, 908
AvaII	GGWCC	1 cut(s)	42
BalI	TGGCCA	1 cut(s)	83
BanI	GGYRCC	2 cut(s)	512, 762
BbvI	GCAGC	2 cut(s)	301, 308
BccI	CCATC	5 cut(s)	131, 211, 454, 503, 681
BciT130I	CCWGG	2 cut(s)	59, 683
BcnI	CCSGG	3 cut(s)	105, 120, 338
BcoDI	GTCTC	3 cut(s)	616, 936, 1014
BfaI	CTAG	1 cut(s)	653
BfmI	CTRYAG	1 cut(s)	841
BfoI	RGCGCY	1 cut(s)	213
BglII	AGATCT	2 cut(s)	649, 1053
BisI	GCNGC	3 cut(s)	315, 322, 726
BlsI	GCNGC	3 cut(s)	316, 323, 727
Bme1390I	CCNGG	5 cut(s)	59, 105, 120, 338, 683
Bme18I	GGWCC	1 cut(s)	42
BmeT110I	CYCGRG	2 cut(s)	257, 908
BmgBI	CACGTC	1 cut(s)	799
BmgT120I	GGNCC	2 cut(s)	42, 1059
BmiI	GGNNCC	5 cut(s)	124, 371, 514, 764, 781
BmrFI	CCNGG	5 cut(s)	59, 105, 120, 338, 683
BmrI	ACTGGG	2 cut(s)	635, 956
BmsI	GCATC	2 cut(s)	149, 687
BmuI	ACTGGG	2 cut(s)	635, 956
BpuEI	CTTGAG	2 cut(s)	398, 538
BpuMI	CCSGG	3 cut(s)	105, 120, 338
Bsa29I	ATCGAT	1 cut(s)	157
BsaBI	GATNNNNATC	2 cut(s)	9, 221
BsaJI	CCNNGG	5 cut(s)	118, 337, 682, 907, 1082
BsaWI	WCCGGW	1 cut(s)	133
BsaXI	ACNNNNNCTCC	4 cut(s)	581, 611, 639, 669
Bsc4I	CCNNNNNNNGG	3 cut(s)	379, 603, 830
Bse118I	RCCGGY	1 cut(s)	1071
Bse1I	ACTGG	4 cut(s)	389, 541, 630, 962
Bse3DI	GCAATG	2 cut(s)	84, 169
Bse8I	GATNNNNATC	2 cut(s)	9, 221
BseBI	CCWGG	2 cut(s)	59, 683
BseCI	ATCGAT	1 cut(s)	157
BseDI	CCNNGG	5 cut(s)	118, 337, 682, 907, 1082
BseGI	GGATG	3 cut(s)	142, 678, 692
BseJI	GATNNNNATC	2 cut(s)	9, 221
BseLI	CCNNNNNNNGG	3 cut(s)	379, 603, 830
BseMI	GCAATG	2 cut(s)	84, 169
BseMII	CTCAG	3 cut(s)	689, 949, 1029
BseNI	ACTGG	4 cut(s)	389, 541, 630, 962
BseRI	GAGGAG	2 cut(s)	599, 792
BseXI	GCAGC	2 cut(s)	301, 308
BshFI	GGCC	2 cut(s)	83, 1061
BshNI	GGYRCC	2 cut(s)	512, 762
BshVI	ATCGAT	1 cut(s)	157
BsiHKCI	CYCGRG	2 cut(s)	257, 908
BsiSI	CCGG	5 cut(s)	105, 120, 134, 337, 1072
BslFI	GGGAC	3 cut(s)	101, 620, 926
BslI	CCNNNNNNNGG	3 cut(s)	379, 603, 830
BsmAI	GTCTC	3 cut(s)	616, 936, 1014
BsmBI	CGTCTC	1 cut(s)	1014
BsmFI	GGGAC	3 cut(s)	101, 620, 926
BsnI	GGCC	2 cut(s)	83, 1061
BsoBI	CYCGRG	2 cut(s)	257, 908
Bsp143I	GATC	5 cut(s)	4, 10, 92, 649, 1053
Bsp19I	CCATGG	1 cut(s)	1082
BspACI	CCGC	4 cut(s)	317, 410, 725, 948
BspANI	GGCC	2 cut(s)	83, 1061
BspCNI	CTCAG	3 cut(s)	690, 948, 1030
BspDI	ATCGAT	1 cut(s)	157
BspHI	TCATGA	1 cut(s)	580
BspLI	GGNNCC	5 cut(s)	124, 371, 514, 764, 781
BspPI	GGATC	3 cut(s)	12, 18, 100
BspT107I	GGYRCC	2 cut(s)	512, 762
BsrDI	GCAATG	2 cut(s)	84, 169
BsrFI	RCCGGY	1 cut(s)	1071
BsrI	ACTGG	4 cut(s)	389, 541, 630, 962
BssAI	RCCGGY	1 cut(s)	1071
BssECI	CCNNGG	5 cut(s)	118, 337, 682, 907, 1082
BssMI	GATC	5 cut(s)	4, 10, 92, 649, 1053
BssT1I	CCWWGG	1 cut(s)	1082
Bst2UI	CCWGG	2 cut(s)	59, 683
Bst4CI	ACNGT	2 cut(s)	899, 930
Bst6I	CTCTTC	1 cut(s)	542
BstC8I	GCNNGC	4 cut(s)	319, 467, 867, 1073
BstDEI	CTNAG	4 cut(s)	213, 698, 935, 1038
BstDSI	CCRYGG	1 cut(s)	1082
BstENI	CCTNNNNNAGG	1 cut(s)	377
BstF5I	GGATG	3 cut(s)	142, 678, 692
BstH2I	RGCGCY	1 cut(s)	213
BstHHI	GCGC	1 cut(s)	212
BstKTI	GATC	5 cut(s)	7, 13, 95, 652, 1056
BstMAI	GTCTC	3 cut(s)	616, 936, 1014
BstMBI	GATC	5 cut(s)	4, 10, 92, 649, 1053
BstMWI	GCNNNNNNNGC	6 cut(s)	59, 193, 409, 734, 945, 1034
BstNI	CCWGG	2 cut(s)	59, 683
BstSCI	CCNGG	5 cut(s)	57, 103, 118, 336, 681
BstSFI	CTRYAG	1 cut(s)	841
BstV1I	GCAGC	2 cut(s)	301, 308
BstX2I	RGATCY	4 cut(s)	4, 92, 649, 1053
BstYI	RGATCY	4 cut(s)	4, 92, 649, 1053
Bsu15I	ATCGAT	1 cut(s)	157
BsuRI	GGCC	2 cut(s)	83, 1061
BsuTUI	ATCGAT	1 cut(s)	157
BtgI	CCRYGG	1 cut(s)	1082
BtrI	CACGTC	1 cut(s)	799
BtsCI	GGATG	3 cut(s)	142, 678, 692
BtsIMutI	CAGTG	2 cut(s)	895, 926
Cac8I	GCNNGC	4 cut(s)	319, 467, 867, 1073
CaiI	CAGNNNCTG	1 cut(s)	728
CciI	TCATGA	1 cut(s)	580
CfoI	GCGC	1 cut(s)	212
Cfr10I	RCCGGY	1 cut(s)	1071
Cfr13I	GGNCC	2 cut(s)	42, 1059
ClaI	ATCGAT	1 cut(s)	157
Csp6I	GTAC	5 cut(s)	305, 513, 589, 763, 1045
CviAII	CATG	7 cut(s)	39, 359, 581, 592, 603, 634, 1083
CviQI	GTAC	5 cut(s)	305, 513, 589, 763, 1045
DdeI	CTNAG	4 cut(s)	213, 698, 935, 1038
DpnI	GATC	5 cut(s)	6, 12, 94, 651, 1055
DpnII	GATC	5 cut(s)	4, 10, 92, 649, 1053
EaeI	YGGCCR	1 cut(s)	81
Eam1104I	CTCTTC	1 cut(s)	542
EarI	CTCTTC	1 cut(s)	542
Eco130I	CCWWGG	1 cut(s)	1082
Eco32I	GATATC	1 cut(s)	223
Eco47I	GGWCC	1 cut(s)	42
Eco57I	CTGAAG	1 cut(s)	371
Eco88I	CYCGRG	2 cut(s)	257, 908
EcoNI	CCTNNNNNAGG	1 cut(s)	377
EcoRII	CCWGG	2 cut(s)	57, 681
EcoRV	GATATC	1 cut(s)	223
EcoT14I	CCWWGG	1 cut(s)	1082
ErhI	CCWWGG	1 cut(s)	1082
Esp3I	CGTCTC	1 cut(s)	1014
FaeI	CATG	7 cut(s)	42, 362, 584, 595, 606, 637, 1086
FalI	AAGNNNNNCTT	2 cut(s)	785, 817
FaqI	GGGAC	3 cut(s)	101, 620, 926
FatI	CATG	7 cut(s)	38, 358, 580, 591, 602, 633, 1082
FauI	CCCGC	2 cut(s)	310, 941
FauNDI	CATATG	2 cut(s)	827, 1004
FblI	GTMKAC	1 cut(s)	329
Fnu4HI	GCNGC	3 cut(s)	315, 322, 726
FokI	GGATG	3 cut(s)	149, 665, 699
Fsp4HI	GCNGC	3 cut(s)	315, 322, 726
FspBI	CTAG	1 cut(s)	653
GlaI	GCGC	1 cut(s)	211
GluI	GCNGC	3 cut(s)	315, 322, 726
HaeII	RGCGCY	1 cut(s)	213
HaeIII	GGCC	2 cut(s)	83, 1061
HapII	CCGG	5 cut(s)	105, 120, 134, 337, 1072
HhaI	GCGC	1 cut(s)	212
Hin1II	CATG	7 cut(s)	42, 362, 584, 595, 606, 637, 1086
Hin6I	GCGC	1 cut(s)	210
HinP1I	GCGC	1 cut(s)	210
HincII	GTYRAC	2 cut(s)	270, 330
HindII	GTYRAC	2 cut(s)	270, 330
HinfI	GANTC	4 cut(s)	255, 520, 661, 811
HpaII	CCGG	5 cut(s)	105, 120, 134, 337, 1072
Hpy166II	GTNNAC	3 cut(s)	270, 330, 669
Hpy188I	TCNGA	4 cut(s)	247, 699, 756, 776
Hpy188III	TCNNGA	5 cut(s)	8, 377, 506, 581, 658
Hpy8I	GTNNAC	3 cut(s)	270, 330, 669
HpyAV	CCTTC	1 cut(s)	383
HpyCH4III	ACNGT	2 cut(s)	899, 930
HpyCH4IV	ACGT	1 cut(s)	798
HpyCH4V	TGCA	5 cut(s)	162, 239, 324, 890, 926
HpyF10VI	GCNNNNNNNGC	6 cut(s)	59, 193, 409, 734, 945, 1034
HpyF3I	CTNAG	4 cut(s)	213, 698, 935, 1038
HpySE526I	ACGT	1 cut(s)	798
Hsp92II	CATG	7 cut(s)	42, 362, 584, 595, 606, 637, 1086
HspAI	GCGC	1 cut(s)	210
KpnI	GGTACC	2 cut(s)	516, 766
KroI	GCCGGC	1 cut(s)	1071
KroNI	GCCGGC	1 cut(s)	1073
Kzo9I	GATC	5 cut(s)	4, 10, 92, 649, 1053
LmnI	GCTCC	2 cut(s)	122, 779
Lsp1109I	GCAGC	2 cut(s)	301, 308
LweI	GCATC	2 cut(s)	149, 687
MaeI	CTAG	1 cut(s)	653
MaeII	ACGT	1 cut(s)	798
MaeIII	GTNAC	3 cut(s)	295, 361, 918
MalI	GATC	5 cut(s)	6, 12, 94, 651, 1055
MboI	GATC	5 cut(s)	4, 10, 92, 649, 1053
MboII	GAAGA	6 cut(s)	8, 61, 336, 529, 820, 980
MflI	RGATCY	4 cut(s)	4, 92, 649, 1053
MlsI	TGGCCA	1 cut(s)	83
MluCI	AATT	6 cut(s)	30, 434, 739, 885, 901, 981
MluNI	TGGCCA	1 cut(s)	83
MlyI	GAGTC	2 cut(s)	249, 529
Mox20I	TGGCCA	1 cut(s)	83
MroNI	GCCGGC	1 cut(s)	1071
MscI	TGGCCA	1 cut(s)	83
MseI	TTAA	3 cut(s)	405, 804, 1108
MslI	CAYNNNNRTG	2 cut(s)	181, 826
Msp20I	TGGCCA	1 cut(s)	83
MspA1I	CMGCKG	1 cut(s)	725
MspI	CCGG	5 cut(s)	105, 120, 134, 337, 1072
MspR9I	CCNGG	5 cut(s)	59, 105, 120, 338, 683
MvaI	CCWGG	2 cut(s)	59, 683
MwoI	GCNNNNNNNGC	6 cut(s)	59, 193, 409, 734, 945, 1034
NaeI	GCCGGC	1 cut(s)	1073
NciI	CCSGG	3 cut(s)	105, 120, 338
NcoI	CCATGG	1 cut(s)	1082
NdeI	CATATG	2 cut(s)	827, 1004
NdeII	GATC	5 cut(s)	4, 10, 92, 649, 1053
NgoMIV	GCCGGC	1 cut(s)	1071
NlaIII	CATG	7 cut(s)	42, 362, 584, 595, 606, 637, 1086
NlaIV	GGNNCC	5 cut(s)	124, 371, 514, 764, 781
NmeAIII	GCCGAG	1 cut(s)	124
NmuCI	GTSAC	2 cut(s)	295, 361
OliI	CACNNNNGTG	1 cut(s)	181
PaeR7I	CTCGAG	1 cut(s)	257
PagI	TCATGA	1 cut(s)	580
PdiI	GCCGGC	1 cut(s)	1073
PfeI	GAWTC	2 cut(s)	661, 811
PkrI	GCNGC	3 cut(s)	316, 323, 727
PleI	GAGTC	2 cut(s)	249, 528
PpsI	GAGTC	2 cut(s)	249, 528
Psp6I	CCWGG	2 cut(s)	57, 681
PspGI	CCWGG	2 cut(s)	57, 681
PspN4I	GGNNCC	5 cut(s)	124, 371, 514, 764, 781
PspPI	GGNCC	2 cut(s)	42, 1059
PspXI	VCTCGAGB	1 cut(s)	257
PstNI	CAGNNNCTG	1 cut(s)	728
PsuI	RGATCY	4 cut(s)	4, 92, 649, 1053
RsaI	GTAC	5 cut(s)	306, 514, 590, 764, 1046
RsaNI	GTAC	5 cut(s)	305, 513, 589, 763, 1045
RseI	CAYNNNNRTG	2 cut(s)	181, 826
SalI	GTCGAC	1 cut(s)	328
SaqAI	TTAA	3 cut(s)	405, 804, 1108
SatI	GCNGC	3 cut(s)	315, 322, 726
Sau3AI	GATC	5 cut(s)	4, 10, 92, 649, 1053
Sau96I	GGNCC	2 cut(s)	42, 1059
SchI	GAGTC	2 cut(s)	249, 529
ScrFI	CCNGG	5 cut(s)	59, 105, 120, 338, 683
SfaNI	GCATC	2 cut(s)	149, 687
SfcI	CTRYAG	1 cut(s)	841
Sfr274I	CTCGAG	1 cut(s)	257
SinI	GGWCC	1 cut(s)	42
SlaI	CTCGAG	1 cut(s)	257
SmiMI	CAYNNNNRTG	2 cut(s)	181, 826
SmlI	CTYRAG	3 cut(s)	257, 377, 517
SmoI	CTYRAG	3 cut(s)	257, 377, 517
Sse9I	AATT	6 cut(s)	30, 434, 739, 885, 901, 981
SsiI	CCGC	4 cut(s)	317, 410, 725, 948
SspI	AATATT	1 cut(s)	1092
SspMI	CTAG	1 cut(s)	653
StyD4I	CCNGG	5 cut(s)	57, 103, 118, 336, 681
StyI	CCWWGG	1 cut(s)	1082
TaaI	ACNGT	2 cut(s)	899, 930
TaiI	ACGT	1 cut(s)	801
TaqI	TCGA	3 cut(s)	157, 258, 329
TasI	AATT	6 cut(s)	30, 434, 739, 885, 901, 981
TatI	WGTACW	2 cut(s)	588, 1044
TauI	GCSGC	1 cut(s)	728
TfiI	GAWTC	2 cut(s)	661, 811
Tru1I	TTAA	3 cut(s)	405, 804, 1108
Tru9I	TTAA	3 cut(s)	405, 804, 1108
TscAI	CASTG	2 cut(s)	902, 933
TseFI	GTSAC	2 cut(s)	295, 361
TseI	GCWGC	2 cut(s)	314, 321
Tsp45I	GTSAC	2 cut(s)	295, 361
TspDTI	ATGAA	3 cut(s)	140, 569, 622
TspGWI	ACGGA	1 cut(s)	654
TspRI	CASTG	2 cut(s)	902, 933
VpaK11BI	GGWCC	1 cut(s)	42
XagI	CCTNNNNNAGG	1 cut(s)	377
XapI	RAATTY	1 cut(s)	434
XhoI	CTCGAG	1 cut(s)	257
XmiI	GTMKAC	1 cut(s)	329
XspI	CTAG	1 cut(s)	653

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.