Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011259

Physical Location & Seq

Genomic Coordinates Forward (+)

1679140 .. 1684739

Gene Structure 5600 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1017500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGGATCTGGGTCATCTTCTCCACCTCCTAATTCCCTCATGGAACTCTGTTGCTATTCTGGCATTGTTCTTCACTTACTTGGCCATTGCTGGATCGATACTGCCCGGAAAACTTGTCCCCGGAGCCACTTTACCGGACGGCTCTCGGCTTCATTATCGATGCAATAGTCTGCTATCACTTCTGGTGCTGGTTGGGCTTCTTGGGGTTGGCGCTAAGATGGATATCGTGTTGCTTAATGCAATATCAGATAGAGGACTAGAGCTGTTATCAACAACTTTTCTGTTTAGTGTTCTGGTCACACTGGTACTTTACGCTGTGGGCTGCAAGTCGACCAACCAGGGTTCTTCCCTAAAACCTCATGTCACAGGGAACCTTCTTGATGACTGGTGCTTTGGAATACAGCTTAATCCCCGGTTTATGGGCATTGACCTCAAATTTTTCTTTGTTAGAGCTGGAATGATGGGCTGGCTATTTATCAACCTTTCGGTTCTCGCCAGAAGTGTTCAAGACGGTACCTTGAGTCAATCAATGATCCTCTATCAGTTGTTCTGTACGTTATACATCTTGGACTACTTTGTTCATGAGGAGTACATGACCTCCACATGGGACATAATCGCAGAGAGACTGGGCTTCATGCTGGTATTTGGAGATCTAGTCTGGATTCCGTTTACTTTCAGTATCCAGGGATGGTGGCTTCTGAGTAACAAGGTGGAGTTAGGAACAGCGGGTATCGTAGCAAATTGCTTGACTTTTCTGATTGGGTACCTAGTATTCCGAGGAGCCAACAAGCAAAAGCATATCTTTAAGAAGAATCCGAAAGCACCCATATGGGGTAAGCCCGCGAGGGTCATTGGGGGAAAGTTGCTTGCTTCTGGTTATTGGGGAATTGCAAGGCACTGTAATTACCTCGGGGACTTGTTACTAGCACTGTCTTTCAGCTTACCTTGCGGGATAAGTTCCCCAGTTCCATACTTCTATCCAATTTATCTTCTTATTCTGCTCATATGGAGAGAAAGAAGAGATGAAGCTCGTTGTGCCGAGAAGTACAGAGAGATCTGGGCCGAATACCGCCGGCTTGTTCCGTGGAGAATATTACCTTACGTTTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0012505 GO:0016020 GO:0016021 GO:0016491 GO:0016627 GO:0016628 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0036094 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048037 GO:0050613 GO:0050661 GO:0050662 GO:0055114 GO:0097159 GO:0098827 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

ERG4_ERG24

Protein Analysis

370

Amino Acids

41.68

Weight (kDa)

9.11

Isoelectric Point (pI)

30.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ERG4_ERG24	PF01222	10 - 369	5.9e-95	Ergosterol biosynthesis ERG4/ERG24 family
DUF1295	PF06966	207 - 316	2.7e-06	Protein of unknown function (DUF1295)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011259)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52940
fragaria_vesca	FvH4_6g17830 FvH4_6g17830
malus_domestica	MD12G1017500.v1.1 MD14G1015200.v1.1
prunus_persica	Prupe.7G118200_v2.0.a1 Prupe.7G118200_v2.0.a1 Prupe.8G104900_v2.0.a1
pyrus_communis	pycom12g01410 pycom14g01270
rosa_chinensis	RchiOBHm_Chr3g0472081
rosa_laevigata	RLG00000024106
rosa_multiflora	Rmu_sc0002532.1_g000014
rosa_roxburghii	Rroxscaffold_6G00409260
rosa_rugosa	Rorug03G0122000
rosa_samantha	Rh3AG173800 Rh3BG199200 Rh3CG197700 Rh3DG175300
rosa_wichuraiana	Rw3G016680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	512, 762
AccB1I	GGYRCC	2 cut(s)	512, 762
AccI	GTMKAC	1 cut(s)	329
AccII	CGCG	1 cut(s)	842
AciI	CCGC	4 cut(s)	725, 840, 948, 1069
AclWI	GGATC	3 cut(s)	12, 100, 526
AcoI	YGGCCR	1 cut(s)	81
AcsI	RAATTY	1 cut(s)	434
AfaI	GTAC	6 cut(s)	306, 514, 553, 590, 764, 1046
AfiI	CCNNNNNNNGG	3 cut(s)	418, 603, 830
AgsI	TTSAA	1 cut(s)	506
AjnI	CCWGG	2 cut(s)	336, 681
AluBI	AGCT	5 cut(s)	262, 403, 452, 939, 1028
AluI	AGCT	5 cut(s)	262, 403, 452, 939, 1028
Alw26I	GTCTC	1 cut(s)	616
AlwI	GGATC	3 cut(s)	12, 100, 526
Ama87I	CYCGRG	1 cut(s)	908
AoxI	GGCC	2 cut(s)	81, 1059
ApeKI	GCWGC	1 cut(s)	321
ApoI	RAATTY	1 cut(s)	434
ArsI	GACNNNNNNTTYG	2 cut(s)	374, 406
Asp718I	GGTACC	2 cut(s)	512, 762
AspLEI	GCGC	1 cut(s)	212
AspS9I	GGNCC	1 cut(s)	1059
AsuC2I	CCSGG	3 cut(s)	105, 120, 412
AvaI	CYCGRG	1 cut(s)	908
BalI	TGGCCA	1 cut(s)	83
BanI	GGYRCC	2 cut(s)	512, 762
BbvI	GCAGC	1 cut(s)	308
BccI	CCATC	3 cut(s)	211, 454, 681
BceAI	ACGGC	1 cut(s)	154
BciT130I	CCWGG	2 cut(s)	338, 683
BciVI	GTATCC	1 cut(s)	689
BcnI	CCSGG	3 cut(s)	105, 120, 412
BcoDI	GTCTC	1 cut(s)	616
BfaI	CTAG	4 cut(s)	257, 653, 767, 923
BfoI	RGCGCY	1 cut(s)	213
BfuI	GTATCC	1 cut(s)	689
BglII	AGATCT	2 cut(s)	649, 1053
BisI	GCNGC	1 cut(s)	322
BlsI	GCNGC	1 cut(s)	323
Bme1390I	CCNGG	5 cut(s)	105, 120, 338, 412, 683
BmeT110I	CYCGRG	1 cut(s)	908
BmgT120I	GGNCC	1 cut(s)	1059
BmiI	GGNNCC	5 cut(s)	124, 371, 514, 764, 781
BmrFI	CCNGG	5 cut(s)	105, 120, 338, 412, 683
BmrI	ACTGGG	2 cut(s)	635, 956
BmsI	GCATC	1 cut(s)	149
BmuI	ACTGGG	2 cut(s)	635, 956
BpuEI	CTTGAG	1 cut(s)	538
BpuMI	CCSGG	3 cut(s)	105, 120, 412
Bsa29I	ATCGAT	2 cut(s)	95, 157
BsaBI	GATNNNNATC	1 cut(s)	221
BsaJI	CCNNGG	7 cut(s)	118, 337, 410, 682, 775, 907, 1082
BsaWI	WCCGGW	1 cut(s)	133
BsaXI	ACNNNNNCTCC	4 cut(s)	581, 611, 639, 669
Bsc4I	CCNNNNNNNGG	3 cut(s)	418, 603, 830
Bse118I	RCCGGY	1 cut(s)	1071
Bse1I	ACTGG	4 cut(s)	306, 389, 630, 962
Bse3DI	GCAATG	1 cut(s)	84
Bse8I	GATNNNNATC	1 cut(s)	221
BseBI	CCWGG	2 cut(s)	338, 683
BseCI	ATCGAT	2 cut(s)	95, 157
BseDI	CCNNGG	7 cut(s)	118, 337, 410, 682, 775, 907, 1082
BseGI	GGATG	1 cut(s)	692
BseJI	GATNNNNATC	1 cut(s)	221
BseLI	CCNNNNNNNGG	3 cut(s)	418, 603, 830
BseMI	GCAATG	1 cut(s)	84
BseMII	CTCAG	1 cut(s)	689
BseNI	ACTGG	4 cut(s)	306, 389, 630, 962
BseRI	GAGGAG	2 cut(s)	599, 792
BseXI	GCAGC	1 cut(s)	308
Bsh1236I	CGCG	1 cut(s)	842
BshFI	GGCC	2 cut(s)	83, 1061
BshNI	GGYRCC	2 cut(s)	512, 762
BshVI	ATCGAT	2 cut(s)	95, 157
BsiHKCI	CYCGRG	1 cut(s)	908
BsiSI	CCGG	5 cut(s)	105, 120, 134, 412, 1072
BslFI	GGGAC	3 cut(s)	101, 620, 926
BslI	CCNNNNNNNGG	3 cut(s)	418, 603, 830
BsmAI	GTCTC	1 cut(s)	616
BsmFI	GGGAC	3 cut(s)	101, 620, 926
BsnI	GGCC	2 cut(s)	83, 1061
BsoBI	CYCGRG	1 cut(s)	908
Bsp143I	GATC	5 cut(s)	4, 92, 531, 649, 1053
BspACI	CCGC	4 cut(s)	725, 840, 948, 1069
BspANI	GGCC	2 cut(s)	83, 1061
BspCNI	CTCAG	1 cut(s)	690
BspDI	ATCGAT	2 cut(s)	95, 157
BspFNI	CGCG	1 cut(s)	842
BspHI	TCATGA	1 cut(s)	580
BspLI	GGNNCC	5 cut(s)	124, 371, 514, 764, 781
BspPI	GGATC	3 cut(s)	12, 100, 526
BspT107I	GGYRCC	2 cut(s)	512, 762
BsrDI	GCAATG	1 cut(s)	84
BsrFI	RCCGGY	1 cut(s)	1071
BsrI	ACTGG	4 cut(s)	306, 389, 630, 962
BssAI	RCCGGY	1 cut(s)	1071
BssECI	CCNNGG	7 cut(s)	118, 337, 410, 682, 775, 907, 1082
BssMI	GATC	5 cut(s)	4, 92, 531, 649, 1053
Bst2UI	CCWGG	2 cut(s)	338, 683
Bst4CI	ACNGT	3 cut(s)	512, 899, 930
Bst6I	CTCTTC	1 cut(s)	1012
BstC8I	GCNNGC	4 cut(s)	467, 840, 867, 1073
BstDEI	CTNAG	2 cut(s)	213, 698
BstDSI	CCRYGG	1 cut(s)	1082
BstF5I	GGATG	1 cut(s)	692
BstFNI	CGCG	1 cut(s)	842
BstH2I	RGCGCY	1 cut(s)	213
BstHHI	GCGC	1 cut(s)	212
BstKTI	GATC	5 cut(s)	7, 95, 534, 652, 1056
BstMAI	GTCTC	1 cut(s)	616
BstMBI	GATC	5 cut(s)	4, 92, 531, 649, 1053
BstMWI	GCNNNNNNNGC	4 cut(s)	59, 193, 945, 1034
BstNI	CCWGG	2 cut(s)	338, 683
BstSCI	CCNGG	5 cut(s)	103, 118, 336, 410, 681
BstUI	CGCG	1 cut(s)	842
BstV1I	GCAGC	1 cut(s)	308
BstX2I	RGATCY	3 cut(s)	4, 649, 1053
BstYI	RGATCY	3 cut(s)	4, 649, 1053
Bsu15I	ATCGAT	2 cut(s)	95, 157
BsuI	GTATCC	1 cut(s)	689
BsuRI	GGCC	2 cut(s)	83, 1061
BsuTUI	ATCGAT	2 cut(s)	95, 157
BtgI	CCRYGG	1 cut(s)	1082
BtsCI	GGATG	1 cut(s)	692
BtsIMutI	CAGTG	3 cut(s)	299, 895, 926
Cac8I	GCNNGC	4 cut(s)	467, 840, 867, 1073
CciI	TCATGA	1 cut(s)	580
CfoI	GCGC	1 cut(s)	212
Cfr10I	RCCGGY	1 cut(s)	1071
Cfr13I	GGNCC	1 cut(s)	1059
ClaI	ATCGAT	2 cut(s)	95, 157
Csp6I	GTAC	6 cut(s)	305, 513, 552, 589, 763, 1045
CviAII	CATG	6 cut(s)	39, 359, 581, 592, 603, 634
CviQI	GTAC	6 cut(s)	305, 513, 552, 589, 763, 1045
DdeI	CTNAG	2 cut(s)	213, 698
DpnI	GATC	5 cut(s)	6, 94, 533, 651, 1055
DpnII	GATC	5 cut(s)	4, 92, 531, 649, 1053
EaeI	YGGCCR	1 cut(s)	81
Eam1104I	CTCTTC	1 cut(s)	1012
EarI	CTCTTC	1 cut(s)	1012
Eco32I	GATATC	1 cut(s)	223
Eco88I	CYCGRG	1 cut(s)	908
EcoRII	CCWGG	2 cut(s)	336, 681
EcoRV	GATATC	1 cut(s)	223
FaeI	CATG	6 cut(s)	42, 362, 584, 595, 606, 637
FalI	AAGNNNNNCTT	2 cut(s)	785, 817
FaqI	GGGAC	3 cut(s)	101, 620, 926
FatI	CATG	6 cut(s)	38, 358, 580, 591, 602, 633
FauI	CCCGC	3 cut(s)	718, 847, 941
FauNDI	CATATG	2 cut(s)	827, 1004
FblI	GTMKAC	1 cut(s)	329
Fnu4HI	GCNGC	1 cut(s)	322
FokI	GGATG	1 cut(s)	699
Fsp4HI	GCNGC	1 cut(s)	322
FspBI	CTAG	4 cut(s)	257, 653, 767, 923
GlaI	GCGC	1 cut(s)	211
GluI	GCNGC	1 cut(s)	322
HaeII	RGCGCY	1 cut(s)	213
HaeIII	GGCC	2 cut(s)	83, 1061
HapII	CCGG	5 cut(s)	105, 120, 134, 412, 1072
HhaI	GCGC	1 cut(s)	212
Hin1II	CATG	6 cut(s)	42, 362, 584, 595, 606, 637
Hin6I	GCGC	1 cut(s)	210
HinP1I	GCGC	1 cut(s)	210
HincII	GTYRAC	1 cut(s)	330
HindII	GTYRAC	1 cut(s)	330
HinfI	GANTC	3 cut(s)	520, 661, 811
HpaII	CCGG	5 cut(s)	105, 120, 134, 412, 1072
Hpy166II	GTNNAC	3 cut(s)	330, 669, 1105
Hpy188I	TCNGA	5 cut(s)	247, 699, 756, 776, 816
Hpy188III	TCNNGA	4 cut(s)	377, 506, 581, 658
Hpy8I	GTNNAC	3 cut(s)	330, 669, 1105
HpyAV	CCTTC	1 cut(s)	383
HpyCH4III	ACNGT	3 cut(s)	512, 899, 930
HpyCH4IV	ACGT	2 cut(s)	554, 1101
HpyCH4V	TGCA	4 cut(s)	162, 239, 324, 890
HpyF10VI	GCNNNNNNNGC	4 cut(s)	59, 193, 945, 1034
HpyF3I	CTNAG	2 cut(s)	213, 698
HpySE526I	ACGT	2 cut(s)	554, 1101
Hsp92II	CATG	6 cut(s)	42, 362, 584, 595, 606, 637
HspAI	GCGC	1 cut(s)	210
KpnI	GGTACC	2 cut(s)	516, 766
KroI	GCCGGC	1 cut(s)	1071
KroNI	GCCGGC	1 cut(s)	1073
Kzo9I	GATC	5 cut(s)	4, 92, 531, 649, 1053
LmnI	GCTCC	2 cut(s)	122, 779
Lsp1109I	GCAGC	1 cut(s)	308
LweI	GCATC	1 cut(s)	149
MaeI	CTAG	4 cut(s)	257, 653, 767, 923
MaeII	ACGT	2 cut(s)	554, 1101
MaeIII	GTNAC	4 cut(s)	295, 361, 701, 918
MalI	GATC	5 cut(s)	6, 94, 533, 651, 1055
MboI	GATC	5 cut(s)	4, 92, 531, 649, 1053
MboII	GAAGA	6 cut(s)	8, 61, 336, 820, 980, 1029
MflI	RGATCY	3 cut(s)	4, 649, 1053
MlsI	TGGCCA	1 cut(s)	83
MluCI	AATT	6 cut(s)	30, 434, 739, 885, 901, 981
MluNI	TGGCCA	1 cut(s)	83
MlyI	GAGTC	1 cut(s)	529
Mox20I	TGGCCA	1 cut(s)	83
MroNI	GCCGGC	1 cut(s)	1071
MscI	TGGCCA	1 cut(s)	83
MseI	TTAA	3 cut(s)	234, 405, 804
MslI	CAYNNNNRTG	1 cut(s)	826
Msp20I	TGGCCA	1 cut(s)	83
MspA1I	CMGCKG	1 cut(s)	725
MspI	CCGG	5 cut(s)	105, 120, 134, 412, 1072
MspR9I	CCNGG	5 cut(s)	105, 120, 338, 412, 683
MvaI	CCWGG	2 cut(s)	338, 683
MvnI	CGCG	1 cut(s)	842
MwoI	GCNNNNNNNGC	4 cut(s)	59, 193, 945, 1034
NaeI	GCCGGC	1 cut(s)	1073
NciI	CCSGG	3 cut(s)	105, 120, 412
NdeI	CATATG	2 cut(s)	827, 1004
NdeII	GATC	5 cut(s)	4, 92, 531, 649, 1053
NgoMIV	GCCGGC	1 cut(s)	1071
NlaIII	CATG	6 cut(s)	42, 362, 584, 595, 606, 637
NlaIV	GGNNCC	5 cut(s)	124, 371, 514, 764, 781
NmeAIII	GCCGAG	2 cut(s)	124, 1063
NmuCI	GTSAC	2 cut(s)	295, 361
PagI	TCATGA	1 cut(s)	580
PdiI	GCCGGC	1 cut(s)	1073
PfeI	GAWTC	2 cut(s)	661, 811
PkrI	GCNGC	1 cut(s)	323
PleI	GAGTC	1 cut(s)	528
PpsI	GAGTC	1 cut(s)	528
Psp6I	CCWGG	2 cut(s)	336, 681
PspGI	CCWGG	2 cut(s)	336, 681
PspN4I	GGNNCC	5 cut(s)	124, 371, 514, 764, 781
PspPI	GGNCC	1 cut(s)	1059
PsuI	RGATCY	3 cut(s)	4, 649, 1053
RsaI	GTAC	6 cut(s)	306, 514, 553, 590, 764, 1046
RsaNI	GTAC	6 cut(s)	305, 513, 552, 589, 763, 1045
RseI	CAYNNNNRTG	1 cut(s)	826
SalI	GTCGAC	1 cut(s)	328
SaqAI	TTAA	3 cut(s)	234, 405, 804
SatI	GCNGC	1 cut(s)	322
Sau3AI	GATC	5 cut(s)	4, 92, 531, 649, 1053
Sau96I	GGNCC	1 cut(s)	1059
SchI	GAGTC	1 cut(s)	529
ScrFI	CCNGG	5 cut(s)	105, 120, 338, 412, 683
SfaNI	GCATC	1 cut(s)	149
SmiMI	CAYNNNNRTG	1 cut(s)	826
SmlI	CTYRAG	1 cut(s)	517
SmoI	CTYRAG	1 cut(s)	517
Sse9I	AATT	6 cut(s)	30, 434, 739, 885, 901, 981
SsiI	CCGC	4 cut(s)	725, 840, 948, 1069
SspI	AATATT	1 cut(s)	1092
SspMI	CTAG	4 cut(s)	257, 653, 767, 923
StyD4I	CCNGG	5 cut(s)	103, 118, 336, 410, 681
TaaI	ACNGT	3 cut(s)	512, 899, 930
TaiI	ACGT	2 cut(s)	557, 1104
TaqI	TCGA	3 cut(s)	95, 157, 329
TasI	AATT	6 cut(s)	30, 434, 739, 885, 901, 981
TatI	WGTACW	2 cut(s)	588, 1044
TfiI	GAWTC	2 cut(s)	661, 811
Tru1I	TTAA	3 cut(s)	234, 405, 804
Tru9I	TTAA	3 cut(s)	234, 405, 804
TscAI	CASTG	3 cut(s)	306, 902, 933
TseFI	GTSAC	2 cut(s)	295, 361
TseI	GCWGC	1 cut(s)	321
Tsp45I	GTSAC	2 cut(s)	295, 361
TspDTI	ATGAA	4 cut(s)	140, 569, 622, 1038
TspGWI	ACGGA	2 cut(s)	654, 1071
TspRI	CASTG	3 cut(s)	306, 902, 933
XapI	RAATTY	1 cut(s)	434
XmiI	GTMKAC	1 cut(s)	329
XspI	CTAG	4 cut(s)	257, 653, 767, 923

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.