AT3G59520

Rhomboid family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016228
Physical Location & Seq
Forward (+)
21991826 .. 21993263
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59520.1

Sequence Viewer

Length: 810 bp
ATGGGAAGACCTTTGTTTTATGATATCATTGAGAAACCAGCAACGAGCTGTATCGTAACGTTATGCAGTGTGATTTGGTTTGTGATTCAGAAGAAGAGTATTGGGTACTCTCAAGTTGGATTGAGTTACGAGACTGCAATCGAAGGGCATTACTGGAGGATGATTACATCTGCTTTATCACATATTAGTGTCTTGCATCTTGTGTTTAACATGAGTGCTTTATGGAGTCTTGGTGTTGTTGAACAGCTAGGTCATGTAGGTCTTGGCACTGCTTATTACCTTCATTACACTCTTGTTCTTGTTGTGTTCTCTGGTGTTTTGGTTATTGGGATCTATCATTTGTTGATTGCTAGATTCAAGATTGATTATTTTCGGCGAGTTACAGCTGTTGGTTACTCTTGTGTTGTCTTTGGTTGGATGACGATTCTCTCTGTTAAGCAACCTTCTTCAAAGCTGAATCTTTTTGGGCTTTTGTCTCTTCCCATTAGCTTTGCGCCATTCGAGTCACTCATTTTCACTTCGATCATTGTTCCACAAGCTAGTTTTCTGGGACATTTGTCGGGAATCCTTGTTGGTTACGCCATTTCGTGGGGTCTGATTGGTGGGATGAACAACTACTGGGCTCTTACTATGCTTGGTTGGATTGTAGTTGTCTTTGTGTTCAGTTTGAAGAAATCGGGTGCTTATGATTTCAGTTTTTTGGAGATCGAATCGCTTACTGATGCTTCTTTACCCTCAGTGCGGTTTATTGGAAATGGCCGGACCTTGCAAGCCAGTGCAGTTCCTCTTAGTGGAGTGGAAGTTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

269

Amino Acids

29.64

Weight (kDa)

8.71

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 49 - 200 1.3e-23 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59520
fragaria_vesca FvH4_6g51270
malus_domestica MD10G1004100.v1.1
prunus_persica Prupe.8G006500_v2.0.a1
pyrus_communis pycom10g00330
rosa_chinensis RchiOBHm_Chr2g0172871
rosa_laevigata RLG00000022133
rosa_multiflora Rmu_sc0002979.1_g000006
rosa_roxburghii Rroxscaffold_2G00079470
rosa_rugosa Rorug02G0562900
rosa_samantha Rh2AG639800 Rh2BG653200 Rh2CG619000 Rh2DG666900
rosa_wichuraiana Rw2G052820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 588
AciI CCGC 1 cut(s) 742
AclI AACGTT 1 cut(s) 59
AclWI GGATC 1 cut(s) 338
AcoI YGGCCR 1 cut(s) 757
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 3 cut(s) 588, 741, 791
AgsI TTSAA 4 cut(s) 242, 358, 450, 670
AluBI AGCT 6 cut(s) 48, 247, 386, 454, 489, 539
AluI AGCT 6 cut(s) 48, 247, 386, 454, 489, 539
Alw26I GTCTC 2 cut(s) 125, 480
AlwI GGATC 1 cut(s) 338
AoxI GGCC 1 cut(s) 757
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 1 cut(s) 762
AvaII GGWCC 1 cut(s) 762
BanII GRGCYC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 13
BcoDI GTCTC 2 cut(s) 125, 480
BfaI CTAG 3 cut(s) 248, 351, 540
Bme18I GGWCC 1 cut(s) 762
BmgT120I GGNCC 1 cut(s) 762
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 2 cut(s) 205, 712
BmuI ACTGGG 1 cut(s) 628
BoxI GACNNNNGTC 1 cut(s) 556
BpiI GAAGAC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 175
BpuEI CTTGAG 1 cut(s) 96
BsaXI ACNNNNNCTCC 3 cut(s) 217, 247, 786
Bsc4I CCNNNNNNNGG 3 cut(s) 588, 741, 791
Bse1I ACTGG 3 cut(s) 158, 623, 774
BseGI GGATG 3 cut(s) 165, 423, 612
BseLI CCNNNNNNNGG 3 cut(s) 588, 741, 791
BseMII CTCAG 1 cut(s) 750
BseNI ACTGG 3 cut(s) 158, 623, 774
BsgI GTGCAG 1 cut(s) 798
BshFI GGCC 1 cut(s) 759
BsiSI CCGG 1 cut(s) 760
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 3 cut(s) 588, 741, 791
BsmAI GTCTC 2 cut(s) 125, 480
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 1 cut(s) 759
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 3 cut(s) 330, 522, 705
BspACI CCGC 1 cut(s) 742
BspANI GGCC 1 cut(s) 759
BspCNI CTCAG 1 cut(s) 749
BspPI GGATC 1 cut(s) 338
BsrI ACTGG 3 cut(s) 158, 623, 774
BssMI GATC 3 cut(s) 330, 522, 705
Bst6I CTCTTC 2 cut(s) 89, 483
BstC8I GCNNGC 1 cut(s) 771
BstDEI CTNAG 2 cut(s) 736, 788
BstF5I GGATG 3 cut(s) 165, 423, 612
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 3 cut(s) 333, 525, 708
BstMAI GTCTC 2 cut(s) 125, 480
BstMBI GATC 3 cut(s) 330, 522, 705
BstPAI GACNNNNGTC 1 cut(s) 556
BstV2I GAAGAC 1 cut(s) 13
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BsuRI GGCC 1 cut(s) 759
BtsCI GGATG 3 cut(s) 165, 423, 612
BtsI GCAGTG 2 cut(s) 73, 267
BtsIMutI CAGTG 4 cut(s) 73, 267, 744, 781
Cac8I GCNNGC 1 cut(s) 771
CfoI GCGC 1 cut(s) 496
Cfr13I GGNCC 1 cut(s) 762
Csp6I GTAC 1 cut(s) 106
CviAII CATG 2 cut(s) 211, 254
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 2 cut(s) 736, 788
DpnI GATC 3 cut(s) 332, 524, 707
DpnII GATC 3 cut(s) 330, 522, 705
EaeI YGGCCR 1 cut(s) 757
Eam1104I CTCTTC 2 cut(s) 89, 483
EarI CTCTTC 2 cut(s) 89, 483
Eco24I GRGCYC 1 cut(s) 625
Eco32I GATATC 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 762
EcoRV GATATC 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 625
FaeI CATG 2 cut(s) 214, 257
FaiI YATR 8 cut(s) 21, 64, 183, 212, 223, 255, 632, 687
FaqI GGGAC 1 cut(s) 564
FatI CATG 2 cut(s) 210, 253
FokI GGATG 3 cut(s) 172, 430, 619
FriOI GRGCYC 1 cut(s) 625
FspBI CTAG 3 cut(s) 248, 351, 540
GlaI GCGC 1 cut(s) 495
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 1 cut(s) 759
HapII CCGG 1 cut(s) 760
HhaI GCGC 1 cut(s) 496
Hin1II CATG 2 cut(s) 214, 257
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HinfI GANTC 8 cut(s) 85, 226, 354, 424, 457, 503, 564, 710
HpaII CCGG 1 cut(s) 760
Hpy188I TCNGA 3 cut(s) 90, 597, 809
Hpy188III TCNNGA 2 cut(s) 358, 561
HpyAV CCTTC 3 cut(s) 137, 290, 453
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 5 cut(s) 66, 137, 196, 769, 779
HpyF3I CTNAG 2 cut(s) 736, 788
HpySE526I ACGT 1 cut(s) 59
Hsp92II CATG 2 cut(s) 214, 257
HspAI GCGC 1 cut(s) 494
Kzo9I GATC 3 cut(s) 330, 522, 705
LpnPI CCDG 7 cut(s) 51, 139, 297, 533, 604, 773, 787
LweI GCATC 2 cut(s) 205, 712
MaeI CTAG 3 cut(s) 248, 351, 540
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 6 cut(s) 55, 125, 379, 392, 504, 575
MalI GATC 3 cut(s) 332, 524, 707
MboI GATC 3 cut(s) 330, 522, 705
MboII GAAGA 6 cut(s) 18, 103, 106, 438, 470, 682
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 1 cut(s) 625
MlyI GAGTC 2 cut(s) 235, 512
MmeI TCCRAC 3 cut(s) 97, 395, 620
MnlI CCTC 3 cut(s) 150, 745, 795
MseI TTAA 2 cut(s) 207, 435
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 1 cut(s) 760
NdeII GATC 3 cut(s) 330, 522, 705
NlaIII CATG 2 cut(s) 214, 257
NmuCI GTSAC 1 cut(s) 504
PfeI GAWTC 6 cut(s) 85, 354, 424, 457, 564, 710
PflMI CCANNNNNTGG 1 cut(s) 588
PleI GAGTC 2 cut(s) 234, 511
PpsI GAGTC 2 cut(s) 234, 511
PshAI GACNNNNGTC 1 cut(s) 556
Psp1406I AACGTT 1 cut(s) 59
PspPI GGNCC 1 cut(s) 762
PsuI RGATCY 1 cut(s) 330
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 2 cut(s) 207, 435
Sau3AI GATC 3 cut(s) 330, 522, 705
Sau96I GGNCC 1 cut(s) 762
SchI GAGTC 2 cut(s) 235, 512
SduI GDGCHC 1 cut(s) 625
SfaNI GCATC 2 cut(s) 205, 712
SinI GGWCC 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
SsiI CCGC 1 cut(s) 742
SspMI CTAG 3 cut(s) 248, 351, 540
TaiI ACGT 1 cut(s) 62
TaqI TCGA 4 cut(s) 141, 501, 521, 708
TfiI GAWTC 6 cut(s) 85, 354, 424, 457, 564, 710
Tru1I TTAA 2 cut(s) 207, 435
Tru9I TTAA 2 cut(s) 207, 435
TscAI CASTG 4 cut(s) 73, 274, 744, 781
TseFI GTSAC 1 cut(s) 504
Tsp45I GTSAC 1 cut(s) 504
TspDTI ATGAA 2 cut(s) 272, 623
TspRI CASTG 4 cut(s) 73, 274, 744, 781
Van91I CCANNNNNTGG 1 cut(s) 588
VpaK11BI GGWCC 1 cut(s) 762
XspI CTAG 3 cut(s) 248, 351, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.