Rh2AG639800

Rhomboid protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0016228
Physical Location & Seq
Reverse (-)
86349907 .. 86350512
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG639800.1

Sequence Viewer

Length: 606 bp
ATGAGCGCACTTTGGAGTCTTGGGGTAGTCGAAAACTTGGGCTCTATAGGGCTTGGTGTGGAGTATTATCTGCATTACACGCTTGTCTTGGTTGTATTGTCTGGGGTGCTGGTTTTGGGGATCTATCATATCTTGATACAGAAATTCAAGCTGGAGTATTTTCGGAGAGTGACGGCTGTTGGGTATTCTTGTGTTGTTTTTGGGTGGATGACTATTCTTTCTGTGAAGCAACCGACTTCCAAGTTGCACCTGTTTGGATTGCTTTCGCTTCCCATTAGTTTTGCACCTTTTGAGTCTCTTATTTTTACTTCAATCATTGTTCCACAAGCAAGTTTTATAGGCCATTTATCTGGAATCATTGTGGGATATGCTATTGCATGGGGTTTAATTCACGGGATGAACAATTACTGGGCAGTTTCCATGTTAGGATGGATTGTGCTGGTTTGCGCCTTCAGTTTAAAGCGATCTGGTGCCTATGATTTCAGTTTTCTTGAGATTGAGTCCGTTGCTGATTCTTCTTTGCCTTCTGTACGGTTTATTGCATCAGGAAATGGTAGAACATTGCAAATGAGTGCATTGCCAGCTGCAGGTGTTGAGCTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

201

Amino Acids

21.91

Weight (kDa)

7.81

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 27 - 130 1.8e-13 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59520
fragaria_vesca FvH4_6g51270
malus_domestica MD10G1004100.v1.1
prunus_persica Prupe.8G006500_v2.0.a1
pyrus_communis pycom10g00330
rosa_chinensis RchiOBHm_Chr2g0172871
rosa_laevigata RLG00000022133
rosa_multiflora Rmu_sc0002979.1_g000006
rosa_roxburghii Rroxscaffold_2G00079470
rosa_rugosa Rorug02G0562900
rosa_samantha Rh2AG639800 Rh2BG653200 Rh2CG619000 Rh2DG666900
rosa_wichuraiana Rw2G052820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 578
Acc36I ACCTGC 1 cut(s) 578
AccB1I GGYRCC 1 cut(s) 470
AclWI GGATC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 436
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 1 cut(s) 587
AgsI TTSAA 2 cut(s) 148, 312
AluBI AGCT 3 cut(s) 151, 584, 598
AluI AGCT 3 cut(s) 151, 584, 598
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 1 cut(s) 128
AoxI GGCC 1 cut(s) 340
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 1 cut(s) 143
AspLEI GCGC 2 cut(s) 8, 449
BanI GGYRCC 1 cut(s) 470
BanII GRGCYC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 571
BccI CCATC 1 cut(s) 423
BceAI ACGGC 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 300
BfmI CTRYAG 2 cut(s) 45, 585
BfuAI ACCTGC 1 cut(s) 578
BisI GCNGC 1 cut(s) 585
BlsI GCNGC 1 cut(s) 586
BmiI GGNNCC 1 cut(s) 472
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 1 cut(s) 551
BmuI ACTGGG 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 512
Bsc4I CCNNNNNNNGG 1 cut(s) 587
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 2 cut(s) 560, 575
BseGI GGATG 3 cut(s) 213, 402, 434
BseLI CCNNNNNNNGG 1 cut(s) 587
BseMI GCAATG 2 cut(s) 560, 575
BseNI ACTGG 1 cut(s) 413
BseXI GCAGC 1 cut(s) 571
BshFI GGCC 1 cut(s) 342
BshNI GGYRCC 1 cut(s) 470
BslI CCNNNNNNNGG 1 cut(s) 587
BsmAI GTCTC 1 cut(s) 300
BsnI GGCC 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 2 cut(s) 120, 464
BspANI GGCC 1 cut(s) 342
BspLI GGNNCC 1 cut(s) 472
BspMAI CTGCAG 1 cut(s) 589
BspMI ACCTGC 1 cut(s) 578
BspPI GGATC 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 470
BsrDI GCAATG 2 cut(s) 560, 575
BsrI ACTGG 1 cut(s) 413
BssMI GATC 2 cut(s) 120, 464
Bst4CI ACNGT 1 cut(s) 534
BstC8I GCNNGC 1 cut(s) 582
BstF5I GGATG 3 cut(s) 213, 402, 434
BstHHI GCGC 2 cut(s) 8, 449
BstKTI GATC 2 cut(s) 123, 467
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 2 cut(s) 120, 464
BstMWI GCNNNNNNNGC 2 cut(s) 79, 581
BstSFI CTRYAG 2 cut(s) 45, 585
BstV1I GCAGC 1 cut(s) 571
BstX2I RGATCY 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 350
BstYI RGATCY 1 cut(s) 120
BsuRI GGCC 1 cut(s) 342
BtsCI GGATG 3 cut(s) 213, 402, 434
BveI ACCTGC 1 cut(s) 578
Cac8I GCNNGC 1 cut(s) 582
CfoI GCGC 2 cut(s) 8, 449
Csp6I GTAC 1 cut(s) 530
CviAII CATG 2 cut(s) 378, 421
CviJI RGCY 7 cut(s) 42, 52, 151, 176, 342, 584, 598
CviKI_1 RGCY 7 cut(s) 42, 52, 151, 176, 342, 584, 598
CviQI GTAC 1 cut(s) 530
DpnI GATC 2 cut(s) 122, 466
DpnII GATC 2 cut(s) 120, 464
DraI TTTAAA 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 44
FaeI CATG 2 cut(s) 381, 424
FaiI YATR 8 cut(s) 47, 129, 338, 369, 379, 422, 477, 604
FatI CATG 2 cut(s) 377, 420
Fnu4HI GCNGC 1 cut(s) 585
FokI GGATG 3 cut(s) 220, 409, 441
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 585
GlaI GCGC 2 cut(s) 7, 448
GluI GCNGC 1 cut(s) 585
GsuI CTGGAG 1 cut(s) 173
HaeIII GGCC 1 cut(s) 342
HhaI GCGC 2 cut(s) 8, 449
Hin1II CATG 2 cut(s) 381, 424
Hin6I GCGC 2 cut(s) 6, 447
HinP1I GCGC 2 cut(s) 6, 447
HinfI GANTC 5 cut(s) 16, 293, 354, 500, 512
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 4 cut(s) 133, 351, 491, 546
HpyAV CCTTC 2 cut(s) 460, 534
HpyCH4III ACNGT 1 cut(s) 534
HpyCH4V TGCA 8 cut(s) 73, 247, 284, 377, 542, 565, 575, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 581
Hsp92II CATG 2 cut(s) 381, 424
HspAI GCGC 2 cut(s) 6, 447
Kzo9I GATC 2 cut(s) 120, 464
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 1 cut(s) 551
MaeIII GTNAC 1 cut(s) 169
MalI GATC 2 cut(s) 122, 466
MboI GATC 2 cut(s) 120, 464
MboII GAAGA 1 cut(s) 507
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 3 cut(s) 143, 387, 403
MlyI GAGTC 3 cut(s) 25, 302, 509
MseI TTAA 2 cut(s) 386, 458
MspA1I CMGCKG 1 cut(s) 584
MwoI GCNNNNNNNGC 2 cut(s) 79, 581
NdeII GATC 2 cut(s) 120, 464
NlaIII CATG 2 cut(s) 381, 424
NlaIV GGNNCC 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 169
PaqCI CACCTGC 1 cut(s) 578
PfeI GAWTC 2 cut(s) 354, 512
PkrI GCNGC 1 cut(s) 586
PleI GAGTC 3 cut(s) 24, 301, 508
PpsI GAGTC 3 cut(s) 24, 301, 508
PspN4I GGNNCC 1 cut(s) 472
PstI CTGCAG 1 cut(s) 589
PsuI RGATCY 1 cut(s) 120
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
SaqAI TTAA 2 cut(s) 386, 458
SatI GCNGC 1 cut(s) 585
Sau3AI GATC 2 cut(s) 120, 464
SchI GAGTC 3 cut(s) 25, 302, 509
SduI GDGCHC 1 cut(s) 44
SetI ASST 6 cut(s) 153, 252, 289, 586, 592, 600
SfaNI GCATC 1 cut(s) 551
SfcI CTRYAG 2 cut(s) 45, 585
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
Sse9I AATT 3 cut(s) 143, 387, 403
TaaI ACNGT 1 cut(s) 534
TaqI TCGA 1 cut(s) 30
TasI AATT 3 cut(s) 143, 387, 403
TfiI GAWTC 2 cut(s) 354, 512
Tru1I TTAA 2 cut(s) 386, 458
Tru9I TTAA 2 cut(s) 386, 458
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 1 cut(s) 413
TspGWI ACGGA 1 cut(s) 493
XapI RAATTY 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.