pycom10g00330

Rhomboid protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0016228
Physical Location & Seq
Reverse (-)
406812 .. 407627
816 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00330.1

Sequence Viewer

Length: 816 bp
ATGGGTAGGCCATTGGTTTTTGAGATATTGGACAAACCAGCCACAAGTTGTTTCATAGGAATATGCAGTGCAATTTGGTTCTACATACAGAAGAAAAACATTGGCTATTCACATGTAGGCTTGAGTTATGAAACTGCGGTTGCAGGGCACCATTGGAGGATTATAACTTCAGCTTTCTCTCATATAAGCATTATTCATCTTGTTTTCAATATGAGTGCCCTTTGGAGTCTTGGGGTAGTAGAAAAGCTGGGTCCCATAGGGCTCGGGGTGGAATATTATCTGCAGTACACGCTTGTCTTGGTTGTTCTATCCGGAGTGCTAGTCTTGGGAATTTACCATTTGTTAATCCAAAAATTCAAGATTGAATATTTTCGGCGAGTGACAGCTGTTGGGTATTCATGTGTTGTTTTTGGGTGGATGACAATTCTTTCGGTGAAGCAACCCACGTCAAAGTTACATCTTTTTGGATTTCTCTCACTTCCCATTAGTTTTGCACCATTTGAGTCACTTATTTTTACCTCAATCATCGTTCCTCAAGCAAGTTTTATTGGCCATTTATCTGGAATCATTGTTGGGTATGCTATTGCGTGGGGTTTAATTCACGGGATGAACAATTACTGGGCGGTTTCCATGTTGGGATGGATTGTGCTTGTTACTGTGTTCAGTTTGAAGCGATCCAGTGCATATGATTTCAGTTTTCTTGAGATTGAACCTGTTACTGATTCTTCATTGCCAGCCGTACGATTTATTGCATCAGGACATGGTAGAACATTGCAAATGAACGCATTACCAGCTTCGGGTGTTGAGCTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

272

Amino Acids

30.0

Weight (kDa)

8.93

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 49 - 200 7.8e-24 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59520
fragaria_vesca FvH4_6g51270
malus_domestica MD10G1004100.v1.1
prunus_persica Prupe.8G006500_v2.0.a1
pyrus_communis pycom10g00330
rosa_chinensis RchiOBHm_Chr2g0172871
rosa_laevigata RLG00000022133
rosa_multiflora Rmu_sc0002979.1_g000006
rosa_roxburghii Rroxscaffold_2G00079470
rosa_rugosa Rorug02G0562900
rosa_samantha Rh2AG639800 Rh2BG653200 Rh2CG619000 Rh2DG666900
rosa_wichuraiana Rw2G052820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 147
AccIII TCCGGA 1 cut(s) 311
AciI CCGC 2 cut(s) 137, 623
AclWI GGATC 1 cut(s) 669
AcoI YGGCCR 1 cut(s) 550
AcsI RAATTY 2 cut(s) 330, 353
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 2 cut(s) 287, 741
AfiI CCNNNNNNNGG 1 cut(s) 797
AflIII ACRYGT 1 cut(s) 112
AgsI TTSAA 5 cut(s) 208, 358, 365, 670, 710
AjiI CACGTC 1 cut(s) 447
AluBI AGCT 5 cut(s) 173, 247, 386, 794, 808
AluI AGCT 5 cut(s) 173, 247, 386, 794, 808
AlwI GGATC 1 cut(s) 669
Ama87I CYCGRG 1 cut(s) 263
Aor13HI TCCGGA 1 cut(s) 311
AoxI GGCC 2 cut(s) 8, 550
ApoI RAATTY 2 cut(s) 330, 353
ArsI GACNNNNNNTTYG 2 cut(s) 412, 444
Asp700I GAANNNNTTC 1 cut(s) 369
AspS9I GGNCC 1 cut(s) 251
AsuHPI GGTGA 1 cut(s) 445
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 1 cut(s) 251
BaeGI GKGCMC 2 cut(s) 150, 220
BalI TGGCCA 1 cut(s) 552
BanI GGYRCC 1 cut(s) 147
BanII GRGCYC 1 cut(s) 264
BccI CCATC 1 cut(s) 633
BceAI ACGGC 1 cut(s) 722
BfaI CTAG 1 cut(s) 320
BfmI CTRYAG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 251
BmeT110I CYCGRG 1 cut(s) 263
BmgBI CACGTC 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 251
BmiI GGNNCC 3 cut(s) 149, 252, 253
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 1 cut(s) 761
BmuI ACTGGG 1 cut(s) 628
BpuEI CTTGAG 3 cut(s) 142, 519, 722
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 797
Bse1I ACTGG 2 cut(s) 623, 678
Bse3DI GCAATG 2 cut(s) 728, 770
BseAI TCCGGA 1 cut(s) 311
BseGI GGATG 3 cut(s) 423, 612, 644
BseLI CCNNNNNNNGG 1 cut(s) 797
BseMI GCAATG 2 cut(s) 728, 770
BseNI ACTGG 2 cut(s) 623, 678
BseSI GKGCMC 2 cut(s) 150, 220
BseYI CCCAGC 1 cut(s) 247
BshFI GGCC 2 cut(s) 10, 552
BshNI GGYRCC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 1 cut(s) 312
BsiWI CGTACG 1 cut(s) 739
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 797
BsmFI GGGAC 1 cut(s) 237
BsnI GGCC 2 cut(s) 10, 552
BsoBI CYCGRG 1 cut(s) 263
Bsp1286I GDGCHC 3 cut(s) 150, 220, 264
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 1 cut(s) 674
BspACI CCGC 2 cut(s) 137, 623
BspANI GGCC 2 cut(s) 10, 552
BspEI TCCGGA 1 cut(s) 311
BspLI GGNNCC 3 cut(s) 149, 252, 253
BspMAI CTGCAG 1 cut(s) 285
BspPI GGATC 1 cut(s) 669
BspT107I GGYRCC 1 cut(s) 147
BsrDI GCAATG 2 cut(s) 728, 770
BsrI ACTGG 2 cut(s) 623, 678
BssMI GATC 1 cut(s) 674
Bst4CI ACNGT 1 cut(s) 658
BstC8I GCNNGC 1 cut(s) 735
BstF5I GGATG 3 cut(s) 423, 612, 644
BstKTI GATC 1 cut(s) 677
BstMBI GATC 1 cut(s) 674
BstMWI GCNNNNNNNGC 2 cut(s) 289, 791
BstNSI RCATGY 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 281
BstSLI GKGCMC 2 cut(s) 150, 220
BstXI CCANNNNNNTGG 1 cut(s) 560
BsuRI GGCC 2 cut(s) 10, 552
BtrI CACGTC 1 cut(s) 447
BtsCI GGATG 3 cut(s) 423, 612, 644
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 2 cut(s) 73, 685
Cac8I GCNNGC 1 cut(s) 735
Cfr13I GGNCC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 286, 740
CviAII CATG 4 cut(s) 113, 399, 631, 761
CviQI GTAC 2 cut(s) 286, 740
DpnI GATC 1 cut(s) 676
DpnII GATC 1 cut(s) 674
EaeI YGGCCR 1 cut(s) 550
Eco24I GRGCYC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 251
EcoT38I GRGCYC 1 cut(s) 264
FaeI CATG 4 cut(s) 116, 402, 634, 764
FaqI GGGAC 1 cut(s) 237
FatI CATG 4 cut(s) 112, 398, 630, 760
FauNDI CATATG 1 cut(s) 685
FokI GGATG 3 cut(s) 430, 619, 651
FriOI GRGCYC 1 cut(s) 264
FspBI CTAG 1 cut(s) 320
GsaI CCCAGC 1 cut(s) 251
HaeIII GGCC 2 cut(s) 10, 552
HapII CCGG 1 cut(s) 312
Hin1II CATG 4 cut(s) 116, 402, 634, 764
HinfI GANTC 4 cut(s) 226, 503, 564, 722
HpaII CCGG 1 cut(s) 312
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 1 cut(s) 288
Hpy188III TCNNGA 5 cut(s) 312, 358, 561, 701, 756
Hpy8I GTNNAC 1 cut(s) 288
HpyCH4III ACNGT 1 cut(s) 658
HpyCH4IV ACGT 1 cut(s) 446
HpyCH4V TGCA 8 cut(s) 66, 71, 143, 283, 494, 683, 752, 775
HpyF10VI GCNNNNNNNGC 2 cut(s) 289, 791
HpySE526I ACGT 1 cut(s) 446
Hsp92II CATG 4 cut(s) 116, 402, 634, 764
KflI GGGWCCC 1 cut(s) 251
Kpn2I TCCGGA 1 cut(s) 311
Kzo9I GATC 1 cut(s) 674
LweI GCATC 1 cut(s) 761
MaeI CTAG 1 cut(s) 320
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 5 cut(s) 379, 453, 504, 652, 715
MalI GATC 1 cut(s) 676
MboI GATC 1 cut(s) 674
MboII GAAGA 2 cut(s) 103, 717
MhlI GDGCHC 3 cut(s) 150, 220, 264
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 6 cut(s) 72, 330, 353, 423, 597, 613
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 2 cut(s) 235, 512
MnlI CCTC 3 cut(s) 150, 529, 543
Mox20I TGGCCA 1 cut(s) 552
MroI TCCGGA 1 cut(s) 311
MroXI GAANNNNTTC 1 cut(s) 369
MscI TGGCCA 1 cut(s) 552
MseI TTAA 2 cut(s) 344, 596
Msp20I TGGCCA 1 cut(s) 552
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 1 cut(s) 312
MwoI GCNNNNNNNGC 2 cut(s) 289, 791
NdeI CATATG 1 cut(s) 685
NdeII GATC 1 cut(s) 674
NlaIII CATG 4 cut(s) 116, 402, 634, 764
NlaIV GGNNCC 3 cut(s) 149, 252, 253
NmuCI GTSAC 2 cut(s) 379, 504
NspI RCATGY 1 cut(s) 116
PciI ACATGT 1 cut(s) 112
PdmI GAANNNNTTC 1 cut(s) 369
PfeI GAWTC 2 cut(s) 564, 722
Pfl23II CGTACG 1 cut(s) 739
PleI GAGTC 2 cut(s) 234, 511
PpsI GAGTC 2 cut(s) 234, 511
PpuMI RGGWCCY 1 cut(s) 251
PscI ACATGT 1 cut(s) 112
PsiI TTATAA 1 cut(s) 164
Psp5II RGGWCCY 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 247
PspLI CGTACG 1 cut(s) 739
PspN4I GGNNCC 3 cut(s) 149, 252, 253
PspPI GGNCC 1 cut(s) 251
PspPPI RGGWCCY 1 cut(s) 251
PstI CTGCAG 1 cut(s) 285
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 287, 741
RsaNI GTAC 2 cut(s) 286, 740
SaqAI TTAA 2 cut(s) 344, 596
Sau3AI GATC 1 cut(s) 674
Sau96I GGNCC 1 cut(s) 251
SchI GAGTC 2 cut(s) 235, 512
SduI GDGCHC 3 cut(s) 150, 220, 264
SetI ASST 8 cut(s) 175, 249, 388, 449, 521, 715, 796, 810
SfaNI GCATC 1 cut(s) 761
SfcI CTRYAG 1 cut(s) 281
SinI GGWCC 1 cut(s) 251
SmlI CTYRAG 3 cut(s) 121, 534, 701
SmoI CTYRAG 3 cut(s) 121, 534, 701
Sse9I AATT 6 cut(s) 72, 330, 353, 423, 597, 613
SsiI CCGC 2 cut(s) 137, 623
SspI AATATT 2 cut(s) 275, 368
SspMI CTAG 1 cut(s) 320
TaaI ACNGT 1 cut(s) 658
TaiI ACGT 1 cut(s) 449
TasI AATT 6 cut(s) 72, 330, 353, 423, 597, 613
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 2 cut(s) 564, 722
Tru1I TTAA 2 cut(s) 344, 596
Tru9I TTAA 2 cut(s) 344, 596
TscAI CASTG 2 cut(s) 73, 685
TseFI GTSAC 2 cut(s) 379, 504
Tsp45I GTSAC 2 cut(s) 379, 504
TspDTI ATGAA 7 cut(s) 43, 144, 185, 387, 623, 717, 794
TspRI CASTG 2 cut(s) 73, 685
VpaK11BI GGWCC 1 cut(s) 251
XapI RAATTY 2 cut(s) 330, 353
XceI RCATGY 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 369
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.