Rh2DG666900

Rhomboid protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0016228
Physical Location & Seq
Reverse (-)
89148813 .. 89151812
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG666900.1

Sequence Viewer

Length: 816 bp
ATGGGTAGGCCATTGGTTTTTGAGATCTTGGAAAAACCAGTTACAAGTTGCTTTATAGGAATATGCAGTGCAATTTGGTTTTACATACAGAAGAAAAATATTGGGTATTCACATGTGGGCTTGAGTTACGAAACTGCCATTGCAGGGCACCATTGGAGGATTATAACTTCAGCTTTTTCCCATATAAGTGTTCTTCATCTTGTTTTCAATATGAGCGCACTTTGGAGTCTTGGGGTAGTCGAAAACTTGGGCTCTATAGGGCTTGGTGTGGAGTATTATCTGCATTACACTCTCGTCTTGGTTGTATTGTCTGGGGTGCTGGTTTTGGGAATATATCATATCTTGATACAGAAATTCAAGCTGGAGTATTTTCGGAGAGTGACGGCTGTTGGGTATTCTTGTGTTGTTTTTGGGTGGATGACGATTCTTTCTGTGAAGCAACCGACGTCCAAGTTGCACCTGTTTGGATTGCTTTCGCTTCCCATTAGTTTTGCACCTTTTGAGTCTCTTATTTTTACTTCAATCATTGTTCCACAAGCAAGTTTTATAGGCCATTTATCTGGAATCATTGTGGGATATGCTATTGCATGGGGTTTAATTCACGGGATGAACAATTACTGGGCAGTTTCCATGTTAGGATGGATTGTGCTGGTTTGCGCCTTCAGTTTAAAGCGATCTGGTGCCTATGATTTCAGTTTTCTTGAGATTGAGTCCGTTGCTGATTCTTCTTTGCCCTCTGTACGGTTTATTGCATCAGGAAATGGTAGAACATTGCAAATGAGTGCATTGCCAGCTGCAGGTGTTGAGCTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

271

Amino Acids

29.84

Weight (kDa)

8.64

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 49 - 200 3.1e-23 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59520
fragaria_vesca FvH4_6g51270
malus_domestica MD10G1004100.v1.1
prunus_persica Prupe.8G006500_v2.0.a1
pyrus_communis pycom10g00330
rosa_chinensis RchiOBHm_Chr2g0172871
rosa_laevigata RLG00000022133
rosa_multiflora Rmu_sc0002979.1_g000006
rosa_roxburghii Rroxscaffold_2G00079470
rosa_rugosa Rorug02G0562900
rosa_samantha Rh2AG639800 Rh2BG653200 Rh2CG619000 Rh2DG666900
rosa_wichuraiana Rw2G052820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 164
AarI CACCTGC 1 cut(s) 788
AatII GACGTC 1 cut(s) 449
Acc36I ACCTGC 1 cut(s) 788
AccB1I GGYRCC 2 cut(s) 147, 680
AcsI RAATTY 1 cut(s) 353
AcuI CTGAAG 2 cut(s) 153, 646
AcyI GRCGYC 1 cut(s) 446
AfaI GTAC 1 cut(s) 741
AfiI CCNNNNNNNGG 3 cut(s) 144, 741, 797
AflIII ACRYGT 1 cut(s) 112
AgsI TTSAA 3 cut(s) 208, 358, 522
AluBI AGCT 4 cut(s) 173, 361, 794, 808
AluI AGCT 4 cut(s) 173, 361, 794, 808
Alw26I GTCTC 1 cut(s) 510
AoxI GGCC 2 cut(s) 8, 550
ApeKI GCWGC 1 cut(s) 794
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 2 cut(s) 218, 659
BaeGI GKGCMC 1 cut(s) 150
BanI GGYRCC 2 cut(s) 147, 680
BanII GRGCYC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 781
BccI CCATC 1 cut(s) 633
BceAI ACGGC 1 cut(s) 399
BcoDI GTCTC 1 cut(s) 510
BfmI CTRYAG 2 cut(s) 255, 795
BfuAI ACCTGC 1 cut(s) 788
BglII AGATCT 1 cut(s) 24
BisI GCNGC 1 cut(s) 795
BlsI GCNGC 1 cut(s) 796
BmiI GGNNCC 2 cut(s) 149, 682
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 1 cut(s) 761
BmuI ACTGGG 1 cut(s) 628
BpmI CTGGAG 1 cut(s) 383
BpuEI CTTGAG 2 cut(s) 142, 722
BsaHI GRCGYC 1 cut(s) 446
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 741, 797
Bse1I ACTGG 2 cut(s) 38, 623
Bse3DI GCAATG 3 cut(s) 138, 770, 785
BseGI GGATG 3 cut(s) 423, 612, 644
BseLI CCNNNNNNNGG 3 cut(s) 144, 741, 797
BseMI GCAATG 3 cut(s) 138, 770, 785
BseNI ACTGG 2 cut(s) 38, 623
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 1 cut(s) 781
BshFI GGCC 2 cut(s) 10, 552
BshNI GGYRCC 2 cut(s) 147, 680
BslI CCNNNNNNNGG 3 cut(s) 144, 741, 797
BsmAI GTCTC 1 cut(s) 510
BsnI GGCC 2 cut(s) 10, 552
Bsp1286I GDGCHC 2 cut(s) 150, 254
Bsp143I GATC 2 cut(s) 24, 674
BspANI GGCC 2 cut(s) 10, 552
BspLI GGNNCC 2 cut(s) 149, 682
BspMAI CTGCAG 1 cut(s) 799
BspMI ACCTGC 1 cut(s) 788
BspT107I GGYRCC 2 cut(s) 147, 680
BsrDI GCAATG 3 cut(s) 138, 770, 785
BsrI ACTGG 2 cut(s) 38, 623
BssMI GATC 2 cut(s) 24, 674
BssNI GRCGYC 1 cut(s) 446
Bst4CI ACNGT 1 cut(s) 744
BstACI GRCGYC 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 792
BstF5I GGATG 3 cut(s) 423, 612, 644
BstHHI GCGC 2 cut(s) 218, 659
BstKTI GATC 2 cut(s) 27, 677
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 2 cut(s) 24, 674
BstMWI GCNNNNNNNGC 1 cut(s) 791
BstNSI RCATGY 1 cut(s) 116
BstSFI CTRYAG 2 cut(s) 255, 795
BstSLI GKGCMC 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 781
BstX2I RGATCY 1 cut(s) 24
BstXI CCANNNNNNTGG 1 cut(s) 560
BstYI RGATCY 1 cut(s) 24
BsuRI GGCC 2 cut(s) 10, 552
BtsCI GGATG 3 cut(s) 423, 612, 644
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 73
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 1 cut(s) 792
CfoI GCGC 2 cut(s) 218, 659
Csp6I GTAC 1 cut(s) 740
CviAII CATG 3 cut(s) 113, 588, 631
CviQI GTAC 1 cut(s) 740
DpnI GATC 2 cut(s) 26, 676
DpnII GATC 2 cut(s) 24, 674
DraI TTTAAA 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 254
Eco57I CTGAAG 2 cut(s) 153, 646
EcoT38I GRGCYC 1 cut(s) 254
FaeI CATG 3 cut(s) 116, 591, 634
FatI CATG 3 cut(s) 112, 587, 630
Fnu4HI GCNGC 1 cut(s) 795
FokI GGATG 3 cut(s) 430, 619, 651
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 1 cut(s) 795
GlaI GCGC 2 cut(s) 217, 658
GluI GCNGC 1 cut(s) 795
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 2 cut(s) 10, 552
HhaI GCGC 2 cut(s) 218, 659
Hin1I GRCGYC 1 cut(s) 446
Hin1II CATG 3 cut(s) 116, 591, 634
Hin6I GCGC 2 cut(s) 216, 657
HinP1I GCGC 2 cut(s) 216, 657
HinfI GANTC 6 cut(s) 226, 424, 503, 564, 710, 722
Hpy188I TCNGA 1 cut(s) 375
Hpy188III TCNNGA 4 cut(s) 343, 561, 701, 756
Hpy99I CGWCG 1 cut(s) 448
HpyAV CCTTC 1 cut(s) 670
HpyCH4III ACNGT 1 cut(s) 744
HpyCH4IV ACGT 1 cut(s) 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 791
HpySE526I ACGT 1 cut(s) 446
Hsp92I GRCGYC 1 cut(s) 446
Hsp92II CATG 3 cut(s) 116, 591, 634
HspAI GCGC 2 cut(s) 216, 657
Kzo9I GATC 2 cut(s) 24, 674
Lsp1109I GCAGC 1 cut(s) 781
LweI GCATC 1 cut(s) 761
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 3 cut(s) 40, 125, 379
MalI GATC 2 cut(s) 26, 676
MboI GATC 2 cut(s) 24, 674
MboII GAAGA 3 cut(s) 103, 185, 717
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 2 cut(s) 150, 254
MluCI AATT 4 cut(s) 72, 353, 597, 613
MlyI GAGTC 3 cut(s) 235, 512, 719
MnlI CCTC 2 cut(s) 150, 745
MseI TTAA 2 cut(s) 596, 668
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 794
MwoI GCNNNNNNNGC 1 cut(s) 791
NdeII GATC 2 cut(s) 24, 674
NlaIII CATG 3 cut(s) 116, 591, 634
NlaIV GGNNCC 2 cut(s) 149, 682
NmuCI GTSAC 1 cut(s) 379
NspI RCATGY 1 cut(s) 116
PaqCI CACCTGC 1 cut(s) 788
PciI ACATGT 1 cut(s) 112
PfeI GAWTC 3 cut(s) 424, 564, 722
PkrI GCNGC 1 cut(s) 796
PleI GAGTC 3 cut(s) 234, 511, 718
PpsI GAGTC 3 cut(s) 234, 511, 718
PscI ACATGT 1 cut(s) 112
PsiI TTATAA 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 149, 682
PstI CTGCAG 1 cut(s) 799
PsuI RGATCY 1 cut(s) 24
PvuII CAGCTG 1 cut(s) 794
RsaI GTAC 1 cut(s) 741
RsaNI GTAC 1 cut(s) 740
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 2 cut(s) 596, 668
SatI GCNGC 1 cut(s) 795
Sau3AI GATC 2 cut(s) 24, 674
SchI GAGTC 3 cut(s) 235, 512, 719
SduI GDGCHC 2 cut(s) 150, 254
SetI ASST 8 cut(s) 175, 363, 449, 462, 499, 796, 802, 810
SfaNI GCATC 1 cut(s) 761
SfcI CTRYAG 2 cut(s) 255, 795
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 2 cut(s) 121, 701
SmoI CTYRAG 2 cut(s) 121, 701
Sse9I AATT 4 cut(s) 72, 353, 597, 613
SspI AATATT 1 cut(s) 100
TaaI ACNGT 1 cut(s) 744
TaiI ACGT 1 cut(s) 449
TaqI TCGA 1 cut(s) 240
TasI AATT 4 cut(s) 72, 353, 597, 613
TfiI GAWTC 3 cut(s) 424, 564, 722
Tru1I TTAA 2 cut(s) 596, 668
Tru9I TTAA 2 cut(s) 596, 668
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 1 cut(s) 794
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 2 cut(s) 185, 623
TspGWI ACGGA 1 cut(s) 703
TspRI CASTG 1 cut(s) 73
XapI RAATTY 1 cut(s) 353
XceI RCATGY 1 cut(s) 116
ZraI GACGTC 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.