Prupe.8G006500_v2.0.a1

Rhomboid protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0016228
Physical Location & Seq
Reverse (-)
515310 .. 516237
928 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G006500.1

Sequence Viewer

Length: 810 bp
ATGGGTAGGCCGTTGGTTTTCGAGATCTTGGAAAAACCAGCCACGAGTTGTTTCATAGGAATATGCAGTGCAATTTGGTTCTACATACAGAAGAAAAACATTGGGTATTCACATGTGGGCTTGAGTTATGAAACTGCCATTGCAGGGCACCATTGGAGGATTATAACTTCAGCTTTTTCTCATATAAGCATTCTTCATCTTGTTTTCAATATGAGTGCACTTTGGAGTCTTGGGGTAGTAGAAAAGTTGGGGCCTATAGGTCTCGGGATGGAATATTATCTGCAGTACACACTTGTCTTGGTTGTACTTTCCGGAGCGCTAGTCTTGGGGATGTACCATATCTTAATCCAAAAATTCAAGATTGAGTATTTTCGGAGAGTTACTGCTGTTGGGTATTCTTGTGTTGTTTTTGGTTGGATGACGATTCTTTCTGTCAAGCAACCTACATCCAAGTTGCATCTTTTTGGATTTCTTTCACTTCCCATTAGTTTTGCTCCATTTGAGTCACTTATTTTTACTTCAATCATCGTTCCTCAAGCAAGTTTTATCGGTCATTTATCTGGAATTGTTGTTGGGTATGCTATCGCATGGGGTTTAATTCATGGTATGAACAACTACTGGGCAGTTTCCATGTTGGGATGGATTGTACTTGTTTCTGTGTTCAGTTTGAAGCGATCTGGTGCATATGATTTCAGTTTTCTTGAGATTGAATCTGTTACTGATCCTTCATTGCCATCTGTACGGTTTGTTGGAAATGGTAGAACATTGCAAATGAGCGCATTACCAGCTACAGGTGTTGAGCTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

270

Amino Acids

29.8

Weight (kDa)

8.93

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59520
fragaria_vesca FvH4_6g51270
malus_domestica MD10G1004100.v1.1
prunus_persica Prupe.8G006500_v2.0.a1
pyrus_communis pycom10g00330
rosa_chinensis RchiOBHm_Chr2g0172871
rosa_laevigata RLG00000022133
rosa_multiflora Rmu_sc0002979.1_g000006
rosa_roxburghii Rroxscaffold_2G00079470
rosa_rugosa Rorug02G0562900
rosa_samantha Rh2AG639800 Rh2BG653200 Rh2CG619000 Rh2DG666900
rosa_wichuraiana Rw2G052820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 147
AccIII TCCGGA 1 cut(s) 311
AclWI GGATC 1 cut(s) 716
AcsI RAATTY 1 cut(s) 353
AcuI CTGAAG 1 cut(s) 153
AfaI GTAC 5 cut(s) 287, 306, 335, 648, 741
AfeI AGCGCT 1 cut(s) 318
AfiI CCNNNNNNNGG 2 cut(s) 144, 791
AflIII ACRYGT 1 cut(s) 112
AgsI TTSAA 5 cut(s) 208, 358, 522, 670, 710
AluBI AGCT 3 cut(s) 173, 788, 802
AluI AGCT 3 cut(s) 173, 788, 802
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 1 cut(s) 266
Alw44I GTGCAC 1 cut(s) 216
AlwI GGATC 1 cut(s) 716
Ama87I CYCGRG 1 cut(s) 263
Aor13HI TCCGGA 1 cut(s) 311
Aor51HI AGCGCT 1 cut(s) 318
AoxI GGCC 2 cut(s) 8, 251
ApaLI GTGCAC 1 cut(s) 216
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 2 cut(s) 319, 779
AspS9I GGNCC 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 263
BaeGI GKGCMC 2 cut(s) 150, 220
BanI GGYRCC 1 cut(s) 147
BauI CACGAG 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 220
BccI CCATC 3 cut(s) 262, 633, 742
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 1 cut(s) 320
BfmI CTRYAG 3 cut(s) 255, 281, 789
BfoI RGCGCY 1 cut(s) 320
BglII AGATCT 1 cut(s) 24
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 251
BmiI GGNNCC 2 cut(s) 149, 252
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 1 cut(s) 466
BmuI ACTGGG 1 cut(s) 628
BpuEI CTTGAG 3 cut(s) 142, 519, 722
BsaI GGTCTC 1 cut(s) 266
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 791
Bse1I ACTGG 1 cut(s) 623
Bse3DI GCAATG 3 cut(s) 138, 728, 764
BseAI TCCGGA 1 cut(s) 311
BseGI GGATG 5 cut(s) 273, 336, 423, 446, 644
BseLI CCNNNNNNNGG 2 cut(s) 144, 791
BseMI GCAATG 3 cut(s) 138, 728, 764
BseNI ACTGG 1 cut(s) 623
BseSI GKGCMC 2 cut(s) 150, 220
BshFI GGCC 2 cut(s) 10, 253
BshNI GGYRCC 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 1 cut(s) 312
BslI CCNNNNNNNGG 2 cut(s) 144, 791
BsmAI GTCTC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 189
BsnI GGCC 2 cut(s) 10, 253
Bso31I GGTCTC 1 cut(s) 266
BsoBI CYCGRG 1 cut(s) 263
Bsp1286I GDGCHC 2 cut(s) 150, 220
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 3 cut(s) 24, 674, 721
BspANI GGCC 2 cut(s) 10, 253
BspEI TCCGGA 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 149, 252
BspMAI CTGCAG 1 cut(s) 285
BspPI GGATC 1 cut(s) 716
BspT107I GGYRCC 1 cut(s) 147
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 3 cut(s) 138, 728, 764
BsrI ACTGG 1 cut(s) 623
BssMI GATC 3 cut(s) 24, 674, 721
BssSI CACGAG 1 cut(s) 43
Bst2BI CACGAG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 744
BstF5I GGATG 5 cut(s) 273, 336, 423, 446, 644
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 2 cut(s) 319, 779
BstKTI GATC 3 cut(s) 27, 677, 724
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 3 cut(s) 24, 674, 721
BstMWI GCNNNNNNNGC 1 cut(s) 785
BstNSI RCATGY 1 cut(s) 116
BstSFI CTRYAG 3 cut(s) 255, 281, 789
BstSLI GKGCMC 2 cut(s) 150, 220
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BsuRI GGCC 2 cut(s) 10, 253
BtsCI GGATG 5 cut(s) 273, 336, 423, 446, 644
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 73
CfoI GCGC 2 cut(s) 319, 779
Cfr13I GGNCC 1 cut(s) 251
Csp6I GTAC 5 cut(s) 286, 305, 334, 647, 740
CviAII CATG 4 cut(s) 113, 588, 602, 631
CviJI RGCY 7 cut(s) 10, 41, 120, 173, 253, 788, 802
CviKI_1 RGCY 7 cut(s) 10, 41, 120, 173, 253, 788, 802
CviQI GTAC 5 cut(s) 286, 305, 334, 647, 740
DpnI GATC 3 cut(s) 26, 676, 723
DpnII GATC 3 cut(s) 24, 674, 721
Eco31I GGTCTC 1 cut(s) 266
Eco47III AGCGCT 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 251
FaeI CATG 4 cut(s) 116, 591, 605, 634
FatI CATG 4 cut(s) 112, 587, 601, 630
FauNDI CATATG 1 cut(s) 685
FokI GGATG 5 cut(s) 280, 343, 430, 433, 651
FspBI CTAG 1 cut(s) 320
GlaI GCGC 2 cut(s) 318, 778
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 2 cut(s) 10, 253
HapII CCGG 1 cut(s) 312
HhaI GCGC 2 cut(s) 319, 779
Hin1II CATG 4 cut(s) 116, 591, 605, 634
Hin6I GCGC 2 cut(s) 317, 777
HinP1I GCGC 2 cut(s) 317, 777
HinfI GANTC 4 cut(s) 226, 424, 503, 710
HpaII CCGG 1 cut(s) 312
Hpy166II GTNNAC 2 cut(s) 218, 288
Hpy188I TCNGA 1 cut(s) 375
Hpy188III TCNNGA 6 cut(s) 22, 265, 312, 358, 561, 701
Hpy8I GTNNAC 2 cut(s) 218, 288
HpyAV CCTTC 1 cut(s) 735
HpyCH4III ACNGT 1 cut(s) 744
HpyCH4V TGCA 8 cut(s) 66, 71, 143, 218, 283, 457, 683, 769
HpyF10VI GCNNNNNNNGC 1 cut(s) 785
Hsp92II CATG 4 cut(s) 116, 591, 605, 634
HspAI GCGC 2 cut(s) 317, 777
Kpn2I TCCGGA 1 cut(s) 311
Kzo9I GATC 3 cut(s) 24, 674, 721
LmnI GCTCC 2 cut(s) 314, 499
LpnPI CCDG 8 cut(s) 51, 129, 325, 546, 604, 663, 777, 798
LweI GCATC 1 cut(s) 466
MaeI CTAG 1 cut(s) 320
MaeIII GTNAC 3 cut(s) 379, 504, 715
MalI GATC 3 cut(s) 26, 676, 723
MboI GATC 3 cut(s) 24, 674, 721
MboII GAAGA 2 cut(s) 103, 185
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 2 cut(s) 150, 220
MluCI AATT 4 cut(s) 72, 353, 564, 597
MlyI GAGTC 2 cut(s) 235, 512
MmeI TCCRAC 2 cut(s) 395, 730
MnlI CCTC 2 cut(s) 150, 543
MroI TCCGGA 1 cut(s) 311
MseI TTAA 2 cut(s) 344, 596
MspI CCGG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 785
NdeI CATATG 1 cut(s) 685
NdeII GATC 3 cut(s) 24, 674, 721
NlaIII CATG 4 cut(s) 116, 591, 605, 634
NlaIV GGNNCC 2 cut(s) 149, 252
NmuCI GTSAC 1 cut(s) 504
NspI RCATGY 1 cut(s) 116
PciI ACATGT 1 cut(s) 112
PctI GAATGC 1 cut(s) 189
PfeI GAWTC 2 cut(s) 424, 710
PleI GAGTC 2 cut(s) 234, 511
PpsI GAGTC 2 cut(s) 234, 511
PscI ACATGT 1 cut(s) 112
PsiI TTATAA 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 149, 252
PspPI GGNCC 1 cut(s) 251
PstI CTGCAG 1 cut(s) 285
PsuI RGATCY 1 cut(s) 24
RsaI GTAC 5 cut(s) 287, 306, 335, 648, 741
RsaNI GTAC 5 cut(s) 286, 305, 334, 647, 740
SaqAI TTAA 2 cut(s) 344, 596
Sau3AI GATC 3 cut(s) 24, 674, 721
Sau96I GGNCC 1 cut(s) 251
SchI GAGTC 2 cut(s) 235, 512
SduI GDGCHC 2 cut(s) 150, 220
SetI ASST 6 cut(s) 175, 262, 445, 790, 796, 804
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 3 cut(s) 255, 281, 789
SmlI CTYRAG 3 cut(s) 121, 534, 701
SmoI CTYRAG 3 cut(s) 121, 534, 701
Sse9I AATT 4 cut(s) 72, 353, 564, 597
SspI AATATT 1 cut(s) 275
SspMI CTAG 1 cut(s) 320
TaaI ACNGT 1 cut(s) 744
TaqI TCGA 1 cut(s) 21
TaqII GACCGA 1 cut(s) 539
TasI AATT 4 cut(s) 72, 353, 564, 597
TatI WGTACW 3 cut(s) 285, 304, 646
TfiI GAWTC 2 cut(s) 424, 710
Tru1I TTAA 2 cut(s) 344, 596
Tru9I TTAA 2 cut(s) 344, 596
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 1 cut(s) 504
Tsp45I GTSAC 1 cut(s) 504
TspDTI ATGAA 6 cut(s) 43, 144, 185, 590, 623, 717
TspRI CASTG 1 cut(s) 73
VneI GTGCAC 1 cut(s) 216
XapI RAATTY 1 cut(s) 353
XceI RCATGY 1 cut(s) 116
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.