AT3G59630

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006348
Physical Location & Seq
Reverse (-)
22025003 .. 22028523
3521 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59630.1

Sequence Viewer

Length: 1476 bp
ATGGAGTTGGAATTTGAATCAAAGTACGAGATTAACCGTACGGCGGAGTTCATTATTAGCAAAAGCTTCACCAGAATTGCTTTACAGTTCCCGGATGAGTTGTTAAAAGATTCGACTAAGGTCGTGAGTGCTCTTAAGAGCAAAACTCGTTTGCTTACTGACAGAGAAGTCAGGTTCTTTGTAATGGCTGACACAACTTATGGTAGTTGCTGTATTGATGAAGTTGGAGCTTTGCATATTGATTCTGAATGTGTTGTTCATTACGGTCAAACATGTCTAAGCCCGACATCAGTTCTTCCAGCATTCTTCGTTTTTGGGAAAGCTTCGATAAACGTCTCCAGCTGTGTTAAGCATTTGATAGATTATGCGTCAAAGAGCGATAAGCCTATCATGATTCTTTATGGTCTAGAATACGCGCATGTAATTCCACAAATCCGAGAAGAATTAGGATTATCGAAGACTGATTCTCAATTATCTGTAGCCAATGTCTTGTGTTCATTCATTAGTCCATCCAAAGATCCTAGAGAATCCATGGAGCATCCGAGACCATATAGCGAAAGTGATTCTAGTGATAGCTTATCCTCATCTAGGAGCTATAGACTAGGAGGGCTAACTTGGGACTTGCCAGAAGGAAGCAAAATCGAGGACTATTTACTTTTCTGGATAGGTTCTGACAGTTCAGCTTTTGCCAATGTAGTACTGACCTTCAATGGTTGTGACATAGTCAGATATGATGCTGAAGAAGATTCTTTGGTGACGGAATTTTATCAACAAAGAAGGATACTTAAGCGACGATATTACTTGGTGGAAAAAGCTAAGGATGCGAATATTATAGGTATTTTGGTGGGGACTCTTGGTGTTGCTGGTTACCTTCATATGATTCACCATATGCAAGCACTCATATCTGCAGCTGGCAAAAAATCTTACATTCTTGCCATGGGACGGCCTAACCCGGCCAAACTCGCAAACTTCCCCGAGTGCGATGTTTTCATCTACATCTCATGTGCCCAAACGGCTCTTTTAGATAGCAAAGAGTTCATGTCCCCTGTCATTACTCCGTTCGAAGCCAATCTTGCTTTTAGCAGAGGGAGTGAATGGACAGGTGCATACCTAATGCATTTCCAAGATGTGATCAACTCTGTAAAATCAGAATCAGAAGCACATATCGGATCAGAGGAGCCACGGTTCTCCTTCTTTCAGGGTGGTTACGTAGAAGATCACAAAACAAATGATCAAGCTAAGAATGGAGAAGAAGACACAGGAGAGACAATGACATTGGTACAAGCTGCAGAGAAAGCATTGCAGTTGCGAGGCAATGATCATAACTCATTGACTAAACAAACCGCTGCAAAATCAGGACCTGAGTATTTCCTTAACCGAGTTTATCGCGGTTTGGAGATAAATAGTGAGAATACTTTGCCGGAGCCTTATATAGTTGGGAGAAGCGGGAAGGCGTCTGGATACAAGCACGAGTAG

Protein Analysis

491

Amino Acids

54.84

Weight (kDa)

5.43

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthamide_syn PF01866 30 - 370 1.3e-80 Putative diphthamide synthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AccII CGCG 2 cut(s) 416, 1389
AciI CCGC 4 cut(s) 44, 1344, 1389, 1446
AclWI GGATC 2 cut(s) 512, 1177
AcoI YGGCCR 1 cut(s) 954
AcsI RAATTY 2 cut(s) 11, 761
AcuI CTGAAG 1 cut(s) 759
AcyI GRCGYC 1 cut(s) 1454
AfaI GTAC 4 cut(s) 26, 40, 699, 1281
AfiI CCNNNNNNNGG 3 cut(s) 43, 588, 942
AflII CTTAAG 2 cut(s) 134, 785
AflIII ACRYGT 1 cut(s) 272
AgsI TTSAA 2 cut(s) 17, 709
AloI GAACNNNNNNTCC 2 cut(s) 1169, 1201
Alw21I GWGCWC 1 cut(s) 133
Alw26I GTCTC 3 cut(s) 340, 538, 1259
AlwI GGATC 2 cut(s) 512, 1177
AlwNI CAGNNNCTG 1 cut(s) 1361
Ama87I CYCGRG 1 cut(s) 974
AoxI GGCC 2 cut(s) 944, 954
ApeKI GCWGC 3 cut(s) 908, 1286, 1346
ApoI RAATTY 2 cut(s) 11, 761
AspLEI GCGC 1 cut(s) 418
AspS9I GGNCC 1 cut(s) 1358
AsuC2I CCSGG 2 cut(s) 92, 953
AsuHPI GGTGA 3 cut(s) 61, 766, 875
AsuII TTCGAA 1 cut(s) 1062
AvaI CYCGRG 1 cut(s) 974
AvaII GGWCC 1 cut(s) 1358
BaeGI GKGCMC 1 cut(s) 1009
BauI CACGAG 1 cut(s) 1469
BbsI GAAGAC 2 cut(s) 464, 1260
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 3 cut(s) 920, 1273, 1333
BccI CCATC 1 cut(s) 517
BceAI ACGGC 3 cut(s) 57, 959, 1029
BciVI GTATCC 2 cut(s) 774, 1454
BclI TGATCA 3 cut(s) 1131, 1231, 1318
BcnI CCSGG 2 cut(s) 92, 953
BcoDI GTCTC 3 cut(s) 340, 538, 1259
BfaI CTAG 5 cut(s) 407, 522, 567, 588, 602
BfmI CTRYAG 4 cut(s) 477, 595, 906, 1287
BfrI CTTAAG 2 cut(s) 134, 785
BfuI GTATCC 2 cut(s) 774, 1454
BglI GCCNNNNNGGC 1 cut(s) 1013
BisI GCNGC 3 cut(s) 909, 1287, 1347
BlsI GCNGC 3 cut(s) 910, 1288, 1348
BmcAI AGTACT 1 cut(s) 699
Bme1390I CCNGG 2 cut(s) 92, 953
Bme18I GGWCC 1 cut(s) 1358
BmeT110I CYCGRG 1 cut(s) 974
BmgT120I GGNCC 1 cut(s) 1358
BmiI GGNNCC 2 cut(s) 1179, 1425
BmrFI CCNGG 2 cut(s) 92, 953
BmsI GCATC 3 cut(s) 547, 724, 811
BoxI GACNNNNGTC 1 cut(s) 119
BpiI GAAGAC 2 cut(s) 464, 1260
BplI GAGNNNNNCTC 2 cut(s) 130, 162
BpmI CTGGAG 1 cut(s) 322
Bpu10I CCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 1062
BpuMI CCSGG 2 cut(s) 92, 953
BsaAI YACGTR 1 cut(s) 1210
BsaHI GRCGYC 1 cut(s) 1454
BsaI GGTCTC 1 cut(s) 538
BsaJI CCNNGG 3 cut(s) 531, 936, 1181
BsaXI ACNNNNNCTCC 2 cut(s) 1169, 1199
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 588, 942
Bse3DI GCAATG 2 cut(s) 1298, 1321
BseDI CCNNGG 3 cut(s) 531, 936, 1181
BseGI GGATG 4 cut(s) 100, 509, 538, 826
BseLI CCNNNNNNNGG 3 cut(s) 43, 588, 942
BseMI GCAATG 2 cut(s) 1298, 1321
BseMII CTCAG 1 cut(s) 1353
BseRI GAGGAG 1 cut(s) 1190
BseSI GKGCMC 1 cut(s) 1009
BseXI GCAGC 3 cut(s) 920, 1273, 1333
Bsh1236I CGCG 2 cut(s) 416, 1389
BshFI GGCC 2 cut(s) 946, 956
BsiHKAI GWGCWC 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 974
BsiSI CCGG 3 cut(s) 92, 953, 1421
BsiWI CGTACG 1 cut(s) 38
BslFI GGGAC 4 cut(s) 632, 862, 954, 1027
BslI CCNNNNNNNGG 3 cut(s) 43, 588, 942
BsmAI GTCTC 3 cut(s) 340, 538, 1259
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 4 cut(s) 632, 862, 954, 1027
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 2 cut(s) 946, 956
Bso31I GGTCTC 1 cut(s) 538
BsoBI CYCGRG 1 cut(s) 974
Bsp119I TTCGAA 1 cut(s) 1062
Bsp1286I GDGCHC 2 cut(s) 133, 1009
Bsp143I GATC 6 cut(s) 517, 1131, 1169, 1216, 1231, 1318
Bsp19I CCATGG 2 cut(s) 531, 936
BspACI CCGC 4 cut(s) 44, 1344, 1389, 1446
BspANI GGCC 2 cut(s) 946, 956
BspCNI CTCAG 1 cut(s) 1354
BspFNI CGCG 2 cut(s) 416, 1389
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 1179, 1425
BspMAI CTGCAG 2 cut(s) 910, 1291
BspPI GGATC 2 cut(s) 512, 1177
BspT104I TTCGAA 1 cut(s) 1062
BspTI CTTAAG 2 cut(s) 134, 785
BspTNI GGTCTC 1 cut(s) 538
BsrDI GCAATG 2 cut(s) 1298, 1321
BssECI CCNNGG 3 cut(s) 531, 936, 1181
BssMI GATC 6 cut(s) 517, 1131, 1169, 1216, 1231, 1318
BssNI GRCGYC 1 cut(s) 1454
BssSI CACGAG 1 cut(s) 1469
BssT1I CCWWGG 2 cut(s) 531, 936
Bst2BI CACGAG 1 cut(s) 1469
Bst4CI ACNGT 5 cut(s) 38, 87, 266, 677, 1185
BstACI GRCGYC 1 cut(s) 1454
BstAFI CTTAAG 2 cut(s) 134, 785
BstBAI YACGTR 1 cut(s) 1210
BstBI TTCGAA 1 cut(s) 1062
BstC8I GCNNGC 2 cut(s) 894, 913
BstDEI CTNAG 5 cut(s) 117, 278, 816, 1239, 1362
BstDSI CCRYGG 3 cut(s) 531, 936, 1181
BstEII GGTNACC 1 cut(s) 866
BstENI CCTNNNNNAGG 1 cut(s) 586
BstF5I GGATG 4 cut(s) 100, 509, 538, 826
BstFNI CGCG 2 cut(s) 416, 1389
BstHHI GCGC 1 cut(s) 418
BstKTI GATC 6 cut(s) 520, 1134, 1172, 1219, 1234, 1321
BstMAI GTCTC 3 cut(s) 340, 538, 1259
BstMBI GATC 6 cut(s) 517, 1131, 1169, 1216, 1231, 1318
BstMWI GCNNNNNNNGC 5 cut(s) 821, 962, 1013, 1073, 1295
BstNSI RCATGY 2 cut(s) 276, 422
BstPAI GACNNNNGTC 1 cut(s) 119
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 2 cut(s) 90, 951
BstSFI CTRYAG 4 cut(s) 477, 595, 906, 1287
BstSLI GKGCMC 1 cut(s) 1009
BstSNI TACGTA 1 cut(s) 1210
BstUI CGCG 2 cut(s) 416, 1389
BstV1I GCAGC 3 cut(s) 920, 1273, 1333
BstV2I GAAGAC 2 cut(s) 464, 1260
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BsuI GTATCC 2 cut(s) 774, 1454
BsuRI GGCC 2 cut(s) 946, 956
BtgI CCRYGG 3 cut(s) 531, 936, 1181
BtgZI GCGATG 1 cut(s) 996
BtsCI GGATG 4 cut(s) 100, 509, 538, 826
Cac8I GCNNGC 2 cut(s) 894, 913
CaiI CAGNNNCTG 1 cut(s) 1361
CciI TCATGA 1 cut(s) 390
CfoI GCGC 1 cut(s) 418
Cfr13I GGNCC 1 cut(s) 1358
CseI GACGC 2 cut(s) 357, 1443
Csp6I GTAC 4 cut(s) 25, 39, 698, 1280
CviAII CATG 7 cut(s) 273, 391, 419, 532, 937, 1002, 1039
CviQI GTAC 4 cut(s) 25, 39, 698, 1280
DdeI CTNAG 5 cut(s) 117, 278, 816, 1239, 1362
DpnI GATC 6 cut(s) 519, 1133, 1171, 1218, 1233, 1320
DpnII GATC 6 cut(s) 517, 1131, 1169, 1216, 1231, 1318
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 1 cut(s) 954
EciI GGCGGA 1 cut(s) 59
Eco105I TACGTA 1 cut(s) 1210
Eco130I CCWWGG 2 cut(s) 531, 936
Eco31I GGTCTC 1 cut(s) 538
Eco47I GGWCC 1 cut(s) 1358
Eco57I CTGAAG 1 cut(s) 759
Eco88I CYCGRG 1 cut(s) 974
Eco91I GGTNACC 1 cut(s) 866
EcoNI CCTNNNNNAGG 1 cut(s) 586
EcoO109I RGGNCCY 1 cut(s) 1358
EcoO65I GGTNACC 1 cut(s) 866
EcoT14I CCWWGG 2 cut(s) 531, 936
EcoT22I ATGCAT 1 cut(s) 1119
ErhI CCWWGG 2 cut(s) 531, 936
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 7 cut(s) 276, 394, 422, 535, 940, 1005, 1042
FalI AAGNNNNNCTT 2 cut(s) 1056, 1088
FaqI GGGAC 4 cut(s) 632, 862, 954, 1027
FatI CATG 7 cut(s) 272, 390, 418, 531, 936, 1001, 1038
FauI CCCGC 1 cut(s) 1439
FauNDI CATATG 2 cut(s) 876, 888
FbaI TGATCA 3 cut(s) 1131, 1231, 1318
Fnu4HI GCNGC 3 cut(s) 909, 1287, 1347
FokI GGATG 4 cut(s) 107, 496, 525, 833
Fsp4HI GCNGC 3 cut(s) 909, 1287, 1347
FspBI CTAG 5 cut(s) 407, 522, 567, 588, 602
GlaI GCGC 1 cut(s) 417
GluI GCNGC 3 cut(s) 909, 1287, 1347
GsuI CTGGAG 1 cut(s) 322
HaeIII GGCC 2 cut(s) 946, 956
HapII CCGG 3 cut(s) 92, 953, 1421
HgaI GACGC 2 cut(s) 357, 1443
HhaI GCGC 1 cut(s) 418
Hin1I GRCGYC 1 cut(s) 1454
Hin1II CATG 7 cut(s) 276, 394, 422, 535, 940, 1005, 1042
Hin6I GCGC 1 cut(s) 416
HinP1I GCGC 1 cut(s) 416
HindIII AAGCTT 2 cut(s) 64, 321
HpaII CCGG 3 cut(s) 92, 953, 1421
HphI GGTGA 3 cut(s) 61, 766, 875
Hpy188I TCNGA 9 cut(s) 247, 437, 543, 673, 728, 1150, 1156, 1169, 1174
Hpy188III TCNNGA 6 cut(s) 124, 391, 407, 661, 1356, 1458
Hpy99I CGWCG 1 cut(s) 795
HpyAV CCTTC 6 cut(s) 623, 715, 771, 881, 1201, 1444
HpyCH4III ACNGT 5 cut(s) 38, 87, 266, 677, 1185
HpyCH4IV ACGT 2 cut(s) 333, 1209
HpyCH4V TGCA 8 cut(s) 235, 892, 908, 1106, 1117, 1289, 1303, 1349
HpyF10VI GCNNNNNNNGC 5 cut(s) 821, 962, 1013, 1073, 1295
HpyF3I CTNAG 5 cut(s) 117, 278, 816, 1239, 1362
HpySE526I ACGT 2 cut(s) 333, 1209
Hsp92I GRCGYC 1 cut(s) 1454
Hsp92II CATG 7 cut(s) 276, 394, 422, 535, 940, 1005, 1042
HspAI GCGC 1 cut(s) 416
Ksp22I TGATCA 3 cut(s) 1131, 1231, 1318
Kzo9I GATC 6 cut(s) 517, 1131, 1169, 1216, 1231, 1318
LmnI GCTCC 5 cut(s) 227, 535, 591, 1177, 1423
Lsp1109I GCAGC 3 cut(s) 920, 1273, 1333
LweI GCATC 3 cut(s) 547, 724, 811
MaeI CTAG 5 cut(s) 407, 522, 567, 588, 602
MaeII ACGT 2 cut(s) 333, 1209
MaeIII GTNAC 4 cut(s) 716, 754, 866, 1205
MalI GATC 6 cut(s) 519, 1133, 1171, 1218, 1233, 1320
MboI GATC 6 cut(s) 517, 1131, 1169, 1216, 1231, 1318
MboII GAAGA 9 cut(s) 287, 298, 452, 469, 752, 755, 1226, 1262, 1265
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 2 cut(s) 133, 1009
MluCI AATT 6 cut(s) 11, 75, 423, 443, 470, 761
MlyI GAGTC 1 cut(s) 844
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 6 cut(s) 592, 599, 637, 1079, 1168, 1304
Mph1103I ATGCAT 1 cut(s) 1119
MseI TTAA 6 cut(s) 33, 104, 135, 348, 786, 1374
MspA1I CMGCKG 3 cut(s) 342, 911, 1346
MspCI CTTAAG 2 cut(s) 134, 785
MspI CCGG 3 cut(s) 92, 953, 1421
MspR9I CCNGG 2 cut(s) 92, 953
Mva1269I GAATGC 1 cut(s) 302
MvnI CGCG 2 cut(s) 416, 1389
MwoI GCNNNNNNNGC 5 cut(s) 821, 962, 1013, 1073, 1295
NciI CCSGG 2 cut(s) 92, 953
NcoI CCATGG 2 cut(s) 531, 936
NdeI CATATG 2 cut(s) 876, 888
NdeII GATC 6 cut(s) 517, 1131, 1169, 1216, 1231, 1318
NlaIII CATG 7 cut(s) 276, 394, 422, 535, 940, 1005, 1042
NlaIV GGNNCC 2 cut(s) 1179, 1425
NmuCI GTSAC 2 cut(s) 716, 754
NsiI ATGCAT 1 cut(s) 1119
NspI RCATGY 2 cut(s) 276, 422
NspV TTCGAA 1 cut(s) 1062
PagI TCATGA 1 cut(s) 390
PciI ACATGT 1 cut(s) 272
PctI GAATGC 1 cut(s) 302
Pfl23II CGTACG 1 cut(s) 38
PflFI GACNNNGTC 1 cut(s) 722
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 3 cut(s) 910, 1288, 1348
PleI GAGTC 1 cut(s) 844
PpsI GAGTC 1 cut(s) 844
Ppu21I YACGTR 1 cut(s) 1210
PpuMI RGGWCCY 1 cut(s) 1358
PscI ACATGT 1 cut(s) 272
PshAI GACNNNNGTC 1 cut(s) 119
Psp5II RGGWCCY 1 cut(s) 1358
PspEI GGTNACC 1 cut(s) 866
PspLI CGTACG 1 cut(s) 38
PspN4I GGNNCC 2 cut(s) 1179, 1425
PspPI GGNCC 1 cut(s) 1358
PspPPI RGGWCCY 1 cut(s) 1358
PstI CTGCAG 2 cut(s) 910, 1291
PstNI CAGNNNCTG 1 cut(s) 1361
PsuI RGATCY 1 cut(s) 517
PsyI GACNNNGTC 1 cut(s) 722
PvuII CAGCTG 2 cut(s) 342, 911
RsaI GTAC 4 cut(s) 26, 40, 699, 1281
RsaNI GTAC 4 cut(s) 25, 39, 698, 1280
SaqAI TTAA 6 cut(s) 33, 104, 135, 348, 786, 1374
SatI GCNGC 3 cut(s) 909, 1287, 1347
Sau3AI GATC 6 cut(s) 517, 1131, 1169, 1216, 1231, 1318
Sau96I GGNCC 1 cut(s) 1358
ScaI AGTACT 1 cut(s) 699
SchI GAGTC 1 cut(s) 844
ScrFI CCNGG 2 cut(s) 92, 953
SduI GDGCHC 2 cut(s) 133, 1009
SfaNI GCATC 3 cut(s) 547, 724, 811
SfcI CTRYAG 4 cut(s) 477, 595, 906, 1287
SfuI TTCGAA 1 cut(s) 1062
SinI GGWCC 1 cut(s) 1358
SmlI CTYRAG 2 cut(s) 134, 785
SmoI CTYRAG 2 cut(s) 134, 785
SnaBI TACGTA 1 cut(s) 1210
Sse9I AATT 6 cut(s) 11, 75, 423, 443, 470, 761
SsiI CCGC 4 cut(s) 44, 1344, 1389, 1446
SspI AATATT 1 cut(s) 829
SspMI CTAG 5 cut(s) 407, 522, 567, 588, 602
StyD4I CCNGG 2 cut(s) 90, 951
StyI CCWWGG 2 cut(s) 531, 936
TaaI ACNGT 5 cut(s) 38, 87, 266, 677, 1185
TaiI ACGT 2 cut(s) 336, 1212
TaqI TCGA 5 cut(s) 113, 326, 455, 642, 1062
TasI AATT 6 cut(s) 11, 75, 423, 443, 470, 761
TatI WGTACW 1 cut(s) 697
Tru1I TTAA 6 cut(s) 33, 104, 135, 348, 786, 1374
Tru9I TTAA 6 cut(s) 33, 104, 135, 348, 786, 1374
TseFI GTSAC 2 cut(s) 716, 754
TseI GCWGC 3 cut(s) 908, 1286, 1346
Tsp45I GTSAC 2 cut(s) 716, 754
TspDTI ATGAA 8 cut(s) 40, 234, 248, 486, 490, 863, 979, 1027
TspGWI ACGGA 2 cut(s) 773, 1047
Tth111I GACNNNGTC 1 cut(s) 722
Vha464I CTTAAG 2 cut(s) 134, 785
VpaK11BI GGWCC 1 cut(s) 1358
XagI CCTNNNNNAGG 1 cut(s) 586
XapI RAATTY 2 cut(s) 11, 761
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 2 cut(s) 276, 422
XspI CTAG 5 cut(s) 407, 522, 567, 588, 602
ZrmI AGTACT 1 cut(s) 699
Zsp2I ATGCAT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.