MD04G1048400.v1.1

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006348
Physical Location & Seq
Reverse (-)
5698957 .. 5703433
4477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1048400.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGGAGTTTGAATCAAATTACGAAATTGCTTCTACCGCCGACTTCATTCACACTAACAACTTCACCAGAGTTGCTCTACAGTTCCCGGATGAGCTTCTGAAGGACTCTGTGAGAGTGGTGAGTGCTCTGAGGGCCAAGCTCCGTTCACTAAGAAAACCCGGCGCCGACCTAAACGACGACGTCCCGGAGGTGTCCTTGTTCGTGATGGCGGACACGGCCTACGGTAGTTGCTGTGTGGACGAGGTGGGAGCGTCGCACGTCGATGCTGATTGTGTTGTTCATTACGGCCACACCTGCCTCAGCCCGACAACGACGCTTCCAGCTTTCTTTGTTTTCGGGAAGGCTCCCATCAGTGTGCTTGATTGTGTCAACAATCTATCACATTATGCTCTCGAAAATCGGAGGCGTGTTTTGGTCCTTTTTGGGCTGGACTATGCACATTCGATTCAGCATATAAAAGAAGAATTTGTACAAGCATGTGGGGTTGGGTCCAAACCAGAAGTTCAATATGCTAATGTTATGTGTTCAGTTATGAATCCATCAAAAGATCGTAAACTCTCAAATGAGCTCCCAGGACCAGCTGGCAGCCTCACTGATGATACCAGTTTTGGGACAACGTCTAGTGTTACTTATAGCATTGGAGGCCTGGTCTGGAAATTACCTGAGGGACGCAAGATGGAGGATTATTTACTTTTCTGGATTGGTTCAAATAACTCCGCCTTTGCAAATATTGTTTTAACATATAATGGATGTGAAATAGTCAGATATGATGCCTCGGAATGCAGTATGGTGACTGACTTATCTCAACAAAGAAGGATTCTTAAGCGCAGATATTACCTAATGGAGAAAGCAAAGGATGCTAACATCGTGGGGATCTTAGTAGGAACCCTTGGTGTAGCTGGTTACCTGAATATGATTCATCAGATGAAAGAGCTGATTACAGGAGCAGGAAAAAAAGTGTATACCCTAGTTATGGGGAGACCAAACCCTGCAAAACTTGCAAACTTTCCAGAGTGTGATGTTTTCATTTATGTCTCTTGCGCTCAAACTGCCCTTCTGGATAGCAAAGAATATTTAGCTCCAGTAATTACTCCTTTTGAAGCCATGATAGCCTTCAACAGAGGATGTGAGTGGACTGGAGCATATGTAATGGAATTCCGCGATTTGATTAGTTCTTCACCAGTGGATATAAGGATCCAGTCTGAGGAAGCACGATTTTCCTTCATACAAGGTGGTTATGTAGAAGATTTCCATCTGCAAGGTAATGGCACTGAGGAAGACAAGGAAGGAGCTCTTGCTTTAGCAAATGCGACAGAGAAGGCTCTGCAATTGCGCGACAACCCTAGTTCTGTAGTGAAAGGAAGAGCTAAATCGGGTGCAGAGTTTTTAGCTACTCGATCATATCAGGGTCTAGATATACATTACGAGACTTCCTTTCCCGAACCATATTTTATTGGGAGGAGTGGAAGGGCAGCAGGGTATAAAGATGAGAAGAACGGGGAAGAGAAATTGTGA

Protein Analysis

505

Amino Acids

55.72

Weight (kDa)

5.37

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthamide_syn PF01866 28 - 382 5e-79 Putative diphthamide synthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 302
AatII GACGTC 1 cut(s) 183
Acc36I ACCTGC 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 962
AccII CGCG 2 cut(s) 1161, 1335
AciI CCGC 4 cut(s) 36, 209, 717, 1159
AclWI GGATC 3 cut(s) 881, 1189, 1202
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 2 cut(s) 464, 1154
AcuI CTGAAG 1 cut(s) 119
AcyI GRCGYC 2 cut(s) 162, 180
AfaI GTAC 1 cut(s) 471
AfiI CCNNNNNNNGG 3 cut(s) 609, 973, 1204
AflII CTTAAG 1 cut(s) 821
AgsI TTSAA 5 cut(s) 11, 506, 708, 1100, 1117
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 2 cut(s) 571, 645
Alw21I GWGCWC 3 cut(s) 127, 570, 1294
Alw26I GTCTC 3 cut(s) 973, 1039, 1421
AlwI GGATC 3 cut(s) 881, 1189, 1202
AoxI GGCC 4 cut(s) 132, 216, 286, 643
ApeKI GCWGC 2 cut(s) 585, 1472
ApoI RAATTY 2 cut(s) 464, 1154
AspLEI GCGC 4 cut(s) 164, 828, 1043, 1335
AspS9I GGNCC 4 cut(s) 132, 415, 489, 575
AsuC2I CCSGG 3 cut(s) 86, 159, 185
AsuHPI GGTGA 4 cut(s) 55, 130, 802, 1170
AvaII GGWCC 3 cut(s) 415, 489, 575
AxyI CCTNAGG 1 cut(s) 663
BamHI GGATCC 1 cut(s) 1194
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 2 cut(s) 570, 1294
BarI GAAGNNNNNNTAC 2 cut(s) 453, 485
BbsI GAAGAC 1 cut(s) 1284
Bbv12I GWGCWC 3 cut(s) 127, 570, 1294
BbvCI CCTCAGC 1 cut(s) 299
BbvI GCAGC 2 cut(s) 597, 1484
BccI CCATC 5 cut(s) 199, 356, 547, 670, 1260
BceAI ACGGC 2 cut(s) 231, 301
BciT130I CCWGG 2 cut(s) 573, 647
BcnI CCSGG 3 cut(s) 86, 159, 185
BcoDI GTCTC 3 cut(s) 973, 1039, 1421
BfaI CTAG 4 cut(s) 621, 968, 1344, 1412
BfmI CTRYAG 2 cut(s) 77, 1350
BfoI RGCGCY 1 cut(s) 165
BfrI CTTAAG 1 cut(s) 821
BfuAI ACCTGC 1 cut(s) 302
BisI GCNGC 2 cut(s) 586, 1473
BlsI GCNGC 2 cut(s) 587, 1474
Bme1390I CCNGG 5 cut(s) 86, 159, 185, 573, 647
Bme18I GGWCC 3 cut(s) 415, 489, 575
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 4 cut(s) 132, 415, 489, 575
BmiI GGNNCC 5 cut(s) 163, 345, 490, 886, 1196
BmrFI CCNGG 5 cut(s) 86, 159, 185, 573, 647
BmsI GCATC 3 cut(s) 253, 760, 847
BpiI GAAGAC 1 cut(s) 1284
BpmI CTGGAG 2 cut(s) 1065, 1158
Bpu10I CCTNAGC 1 cut(s) 299
BpuMI CCSGG 3 cut(s) 86, 159, 185
BsaBI GATNNNNATC 1 cut(s) 1251
BsaHI GRCGYC 2 cut(s) 162, 180
BsaI GGTCTC 1 cut(s) 973
BsaJI CCNNGG 3 cut(s) 571, 774, 889
BsaXI ACNNNNNCTCC 4 cut(s) 633, 663, 1131, 1161
Bsc4I CCNNNNNNNGG 3 cut(s) 609, 973, 1204
Bse1I ACTGG 5 cut(s) 603, 1082, 1141, 1181, 1198
Bse21I CCTNAGG 1 cut(s) 663
Bse8I GATNNNNATC 1 cut(s) 1251
BseBI CCWGG 2 cut(s) 573, 647
BseDI CCNNGG 3 cut(s) 571, 774, 889
BseGI GGATG 4 cut(s) 94, 755, 862, 1130
BseJI GATNNNNATC 1 cut(s) 1251
BseLI CCNNNNNNNGG 3 cut(s) 609, 973, 1204
BseMII CTCAG 5 cut(s) 119, 313, 654, 1194, 1263
BseNI ACTGG 5 cut(s) 603, 1082, 1141, 1181, 1198
BseRI GAGGAG 1 cut(s) 1474
BseXI GCAGC 2 cut(s) 597, 1484
BsgI GTGCAG 1 cut(s) 1398
Bsh1236I CGCG 2 cut(s) 1161, 1335
BshFI GGCC 4 cut(s) 134, 218, 288, 645
BshNI GGYRCC 1 cut(s) 161
BsiHKAI GWGCWC 3 cut(s) 127, 570, 1294
BsiSI CCGG 3 cut(s) 86, 159, 185
BslFI GGGAC 3 cut(s) 167, 625, 681
BslI CCNNNNNNNGG 3 cut(s) 609, 973, 1204
BsmAI GTCTC 3 cut(s) 973, 1039, 1421
BsmFI GGGAC 3 cut(s) 167, 625, 681
BsmI GAATGC 1 cut(s) 785
BsnI GGCC 4 cut(s) 134, 218, 288, 645
Bso31I GGTCTC 1 cut(s) 973
Bsp1286I GDGCHC 3 cut(s) 127, 570, 1294
Bsp1407I TGTACA 1 cut(s) 469
Bsp143I GATC 4 cut(s) 547, 873, 1194, 1397
BspACI CCGC 4 cut(s) 36, 209, 717, 1159
BspANI GGCC 4 cut(s) 134, 218, 288, 645
BspCNI CTCAG 5 cut(s) 120, 312, 655, 1195, 1264
BspFNI CGCG 2 cut(s) 1161, 1335
BspLI GGNNCC 5 cut(s) 163, 345, 490, 886, 1196
BspMI ACCTGC 1 cut(s) 302
BspPI GGATC 3 cut(s) 881, 1189, 1202
BspQI GCTCTTC 1 cut(s) 1357
BspT107I GGYRCC 1 cut(s) 161
BspTI CTTAAG 1 cut(s) 821
BspTNI GGTCTC 1 cut(s) 973
BsrGI TGTACA 1 cut(s) 469
BsrI ACTGG 5 cut(s) 603, 1082, 1141, 1181, 1198
BssECI CCNNGG 3 cut(s) 571, 774, 889
BssMI GATC 4 cut(s) 547, 873, 1194, 1397
BssNAI GTATAC 1 cut(s) 963
BssNI GRCGYC 2 cut(s) 162, 180
BssT1I CCWWGG 1 cut(s) 889
Bst1107I GTATAC 1 cut(s) 963
Bst2UI CCWGG 2 cut(s) 573, 647
Bst4CI ACNGT 2 cut(s) 81, 224
Bst6I CTCTTC 2 cut(s) 1357, 1497
BstACI GRCGYC 2 cut(s) 162, 180
BstAFI CTTAAG 1 cut(s) 821
BstAPI GCANNNNNTGC 2 cut(s) 857, 998
BstAUI TGTACA 1 cut(s) 469
BstC8I GCNNGC 1 cut(s) 583
BstDEI CTNAG 7 cut(s) 128, 149, 299, 663, 877, 1203, 1272
BstEII GGTNACC 1 cut(s) 902
BstF5I GGATG 4 cut(s) 94, 755, 862, 1130
BstFNI CGCG 2 cut(s) 1161, 1335
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 4 cut(s) 164, 828, 1043, 1335
BstKTI GATC 4 cut(s) 550, 876, 1197, 1400
BstMAI GTCTC 3 cut(s) 973, 1039, 1421
BstMBI GATC 4 cut(s) 547, 873, 1194, 1397
BstMWI GCNNNNNNNGC 9 cut(s) 35, 131, 215, 294, 642, 857, 998, 1049, 1109
BstNI CCWGG 2 cut(s) 573, 647
BstNSI RCATGY 1 cut(s) 480
BstPI GGTNACC 1 cut(s) 902
BstSCI CCNGG 5 cut(s) 84, 157, 183, 571, 645
BstSFI CTRYAG 2 cut(s) 77, 1350
BstUI CGCG 2 cut(s) 1161, 1335
BstV1I GCAGC 2 cut(s) 597, 1484
BstV2I GAAGAC 1 cut(s) 1284
BstX2I RGATCY 2 cut(s) 873, 1194
BstYI RGATCY 2 cut(s) 873, 1194
BstZ17I GTATAC 1 cut(s) 963
Bsu36I CCTNAGG 1 cut(s) 663
BsuRI GGCC 4 cut(s) 134, 218, 288, 645
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 4 cut(s) 94, 755, 862, 1130
BtsIMutI CAGTG 4 cut(s) 358, 591, 1188, 1269
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 583
CfoI GCGC 4 cut(s) 164, 828, 1043, 1335
Cfr13I GGNCC 4 cut(s) 132, 415, 489, 575
CseI GACGC 3 cut(s) 240, 322, 678
Csp6I GTAC 1 cut(s) 470
CviAII CATG 2 cut(s) 477, 1105
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 7 cut(s) 128, 149, 299, 663, 877, 1203, 1272
DinI GGCGCC 1 cut(s) 163
DpnI GATC 4 cut(s) 549, 875, 1196, 1399
DpnII GATC 4 cut(s) 547, 873, 1194, 1397
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 1357, 1497
EarI CTCTTC 2 cut(s) 1357, 1497
EciI GGCGGA 2 cut(s) 224, 706
Ecl136II GAGCTC 2 cut(s) 568, 1292
Eco130I CCWWGG 1 cut(s) 889
Eco147I AGGCCT 1 cut(s) 645
Eco24I GRGCYC 2 cut(s) 570, 1294
Eco31I GGTCTC 1 cut(s) 973
Eco47I GGWCC 3 cut(s) 415, 489, 575
Eco53kI GAGCTC 2 cut(s) 568, 1292
Eco57I CTGAAG 1 cut(s) 119
Eco81I CCTNAGG 1 cut(s) 663
Eco91I GGTNACC 1 cut(s) 902
EcoICRI GAGCTC 2 cut(s) 568, 1292
EcoO65I GGTNACC 1 cut(s) 902
EcoRI GAATTC 1 cut(s) 1154
EcoRII CCWGG 2 cut(s) 571, 645
EcoT14I CCWWGG 1 cut(s) 889
EcoT38I GRGCYC 2 cut(s) 570, 1294
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 1 cut(s) 889
FaeI CATG 2 cut(s) 480, 1108
FalI AAGNNNNNCTT 2 cut(s) 1278, 1310
FaqI GGGAC 3 cut(s) 167, 625, 681
FatI CATG 2 cut(s) 476, 1104
FauNDI CATATG 1 cut(s) 1144
FblI GTMKAC 1 cut(s) 962
Fnu4HI GCNGC 2 cut(s) 586, 1473
FokI GGATG 4 cut(s) 101, 762, 869, 1137
FriOI GRGCYC 2 cut(s) 570, 1294
Fsp4HI GCNGC 2 cut(s) 586, 1473
FspBI CTAG 4 cut(s) 621, 968, 1344, 1412
GlaI GCGC 4 cut(s) 163, 827, 1042, 1334
GluI GCNGC 2 cut(s) 586, 1473
GsuI CTGGAG 2 cut(s) 1065, 1158
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 4 cut(s) 134, 218, 288, 645
HapII CCGG 3 cut(s) 86, 159, 185
HgaI GACGC 3 cut(s) 240, 322, 678
HhaI GCGC 4 cut(s) 164, 828, 1043, 1335
Hin1I GRCGYC 2 cut(s) 162, 180
Hin1II CATG 2 cut(s) 480, 1108
Hin6I GCGC 4 cut(s) 162, 826, 1041, 1333
HinP1I GCGC 4 cut(s) 162, 826, 1041, 1333
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 6 cut(s) 11, 104, 445, 535, 817, 916
HpaII CCGG 3 cut(s) 86, 159, 185
HphI GGTGA 4 cut(s) 55, 130, 802, 1170
Hpy166II GTNNAC 6 cut(s) 146, 238, 370, 554, 963, 1134
Hpy188I TCNGA 7 cut(s) 99, 129, 402, 764, 778, 924, 1204
Hpy188III TCNNGA 9 cut(s) 202, 337, 392, 652, 697, 1010, 1058, 1412, 1439
Hpy8I GTNNAC 6 cut(s) 146, 238, 370, 554, 963, 1134
Hpy99I CGWCG 5 cut(s) 179, 182, 256, 263, 316
HpyAV CCTTC 9 cut(s) 94, 334, 807, 1064, 1123, 1231, 1280, 1312, 1461
HpyCH4III ACNGT 2 cut(s) 81, 224
HpyCH4IV ACGT 3 cut(s) 180, 258, 617
HpyCH4V TGCA 8 cut(s) 437, 725, 783, 992, 1001, 1258, 1327, 1379
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 131, 215, 294, 642, 857, 998, 1049, 1109
HpyF3I CTNAG 7 cut(s) 128, 149, 299, 663, 877, 1203, 1272
HpySE526I ACGT 3 cut(s) 180, 258, 617
Hsp92I GRCGYC 2 cut(s) 162, 180
Hsp92II CATG 2 cut(s) 480, 1108
HspAI GCGC 4 cut(s) 162, 826, 1041, 1333
KasI GGCGCC 1 cut(s) 161
Kzo9I GATC 4 cut(s) 547, 873, 1194, 1397
LguI GCTCTTC 1 cut(s) 1357
LmnI GCTCC 8 cut(s) 144, 248, 349, 573, 944, 1084, 1139, 1289
Lsp1109I GCAGC 2 cut(s) 597, 1484
LweI GCATC 3 cut(s) 253, 760, 847
MaeI CTAG 4 cut(s) 621, 968, 1344, 1412
MaeII ACGT 3 cut(s) 180, 258, 617
MaeIII GTNAC 3 cut(s) 625, 790, 902
MalI GATC 4 cut(s) 549, 875, 1196, 1399
MboI GATC 4 cut(s) 547, 873, 1194, 1397
MboII GAAGA 7 cut(s) 473, 1167, 1256, 1289, 1374, 1504, 1514
MfeI CAATTG 1 cut(s) 1328
MflI RGATCY 2 cut(s) 873, 1194
MhlI GDGCHC 3 cut(s) 127, 570, 1294
MluCI AATT 8 cut(s) 16, 24, 464, 656, 1086, 1154, 1328, 1508
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 98
MseI TTAA 2 cut(s) 737, 822
MslI CAYNNNNRTG 2 cut(s) 261, 353
MspA1I CMGCKG 1 cut(s) 581
MspCI CTTAAG 1 cut(s) 821
MspI CCGG 3 cut(s) 86, 159, 185
MspR9I CCNGG 5 cut(s) 86, 159, 185, 573, 647
MunI CAATTG 1 cut(s) 1328
Mva1269I GAATGC 1 cut(s) 785
MvaI CCWGG 2 cut(s) 573, 647
MvnI CGCG 2 cut(s) 1161, 1335
MwoI GCNNNNNNNGC 9 cut(s) 35, 131, 215, 294, 642, 857, 998, 1049, 1109
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 3 cut(s) 86, 159, 185
NdeI CATATG 1 cut(s) 1144
NdeII GATC 4 cut(s) 547, 873, 1194, 1397
NlaIII CATG 2 cut(s) 480, 1108
NlaIV GGNNCC 5 cut(s) 163, 345, 490, 886, 1196
NmuCI GTSAC 1 cut(s) 790
NspI RCATGY 1 cut(s) 480
PaqCI CACCTGC 1 cut(s) 302
PceI AGGCCT 1 cut(s) 645
PciSI GCTCTTC 1 cut(s) 1357
PctI GAATGC 1 cut(s) 785
PfeI GAWTC 5 cut(s) 11, 445, 535, 817, 916
PflFI GACNNNGTC 2 cut(s) 179, 616
PfoI TCCNGGA 2 cut(s) 84, 183
PkrI GCNGC 2 cut(s) 587, 1474
PleI GAGTC 1 cut(s) 98
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 98
Psp124BI GAGCTC 2 cut(s) 570, 1294
Psp6I CCWGG 2 cut(s) 571, 645
PspEI GGTNACC 1 cut(s) 902
PspGI CCWGG 2 cut(s) 571, 645
PspN4I GGNNCC 5 cut(s) 163, 345, 490, 886, 1196
PspPI GGNCC 4 cut(s) 132, 415, 489, 575
PsuI RGATCY 2 cut(s) 873, 1194
PsyI GACNNNGTC 2 cut(s) 179, 616
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
RseI CAYNNNNRTG 2 cut(s) 261, 353
SacI GAGCTC 2 cut(s) 570, 1294
SapI GCTCTTC 1 cut(s) 1357
SaqAI TTAA 2 cut(s) 737, 822
SatI GCNGC 2 cut(s) 586, 1473
Sau3AI GATC 4 cut(s) 547, 873, 1194, 1397
Sau96I GGNCC 4 cut(s) 132, 415, 489, 575
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 5 cut(s) 86, 159, 185, 573, 647
SduI GDGCHC 3 cut(s) 127, 570, 1294
SfaNI GCATC 3 cut(s) 253, 760, 847
SfcI CTRYAG 2 cut(s) 77, 1350
SfoI GGCGCC 1 cut(s) 163
SinI GGWCC 3 cut(s) 415, 489, 575
SmiMI CAYNNNNRTG 2 cut(s) 261, 353
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
Sse9I AATT 8 cut(s) 16, 24, 464, 656, 1086, 1154, 1328, 1508
SseBI AGGCCT 1 cut(s) 645
SsiI CCGC 4 cut(s) 36, 209, 717, 1159
SspDI GGCGCC 1 cut(s) 161
SspI AATATT 2 cut(s) 730, 1073
SspMI CTAG 4 cut(s) 621, 968, 1344, 1412
SstI GAGCTC 2 cut(s) 570, 1294
StuI AGGCCT 1 cut(s) 645
StyD4I CCNGG 5 cut(s) 84, 157, 183, 571, 645
StyI CCWWGG 1 cut(s) 889
TaaI ACNGT 2 cut(s) 81, 224
TaiI ACGT 3 cut(s) 183, 261, 620
TaqI TCGA 4 cut(s) 261, 393, 443, 1396
TasI AATT 8 cut(s) 16, 24, 464, 656, 1086, 1154, 1328, 1508
TatI WGTACW 1 cut(s) 469
TfiI GAWTC 5 cut(s) 11, 445, 535, 817, 916
Tru1I TTAA 2 cut(s) 737, 822
Tru9I TTAA 2 cut(s) 737, 822
TscAI CASTG 4 cut(s) 358, 598, 1188, 1276
TseFI GTSAC 1 cut(s) 790
TseI GCWGC 2 cut(s) 585, 1472
Tsp45I GTSAC 1 cut(s) 790
TspDTI ATGAA 7 cut(s) 34, 269, 548, 908, 941, 1015, 1213
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 4 cut(s) 358, 598, 1188, 1276
Tth111I GACNNNGTC 2 cut(s) 179, 616
Vha464I CTTAAG 1 cut(s) 821
VpaK11BI GGWCC 3 cut(s) 415, 489, 575
XapI RAATTY 2 cut(s) 464, 1154
XbaI TCTAGA 1 cut(s) 1411
XceI RCATGY 1 cut(s) 480
XmiI GTMKAC 1 cut(s) 962
XspI CTAG 4 cut(s) 621, 968, 1344, 1412
ZraI GACGTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.