Rh4CG144500

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006348
Physical Location & Seq
Forward (+)
28854452 .. 28859356
4905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG144500.1

Sequence Viewer

Length: 1485 bp
ATGGAGTTGGAATCAAGTTACGAAATTGGGAGGACAGCGGAGTTCATTGAGAGTAATAACTTCAGGAGAGTGGCTTTACAGTTCCCGGATGAGCTTCTGAAGGAGTCGGTGAGAGTTGTGAGTGCTCTGAGGGCCTCACTCAACAACAAGGAGGAAGTGGGGCTGTTTCTAATGGCGGACACGGCCTACGGTAGTTGCTGTGTGGACGAAGTTGGAGCATCGCATGTCGACGCCGATTGCGTTGTTCATTATGGCCACACCTGCCTCAGCCCGACAACAAAGCTTCCGGCATTGTTTGTTTTTGGGAAGGCTCCAATCACTGTATCTGATTGCGCAAACAAGCTGTCACATTATGCTCAACCAAGTGGAAAGCCTGTTTTGGTTCTTTTTGGGTTGGATTACGCACATTCCATACAGCAAATAAGAGAAACTGTCATGGCAGCAGGTGGATCCGAGTGCAAGCGAGAAGTTCAATTTGCTAATGTAATGTGTTCAGTTATGAATCCATCAAAAGATCCTCAAGTCTCCAATGAAATCGTAGGAGCAGCTGATGGACTTACTAGTAGTAACAGTTTTGGGACGGTATCTGGTAATGTTTATAGAATTGGAGGCTTGGTCTGGGAATTACCTGAGGGACGCAAAATGGAGGATTACTTACTTTTCTGGATCGGTTCCGATAACTCAGCCTTTGCAAACATTGTGTTAACATATAGTGGCTGTGAAATAGTCAGATATGATGCATCGGAGTGCAGTATGGTCACCGACTTGTCTCAACAAAGAAGAATTCTTAAGCGGAGATATTACCTAATGGAAAAAGCAAAGGATGCTAACATTGTTGCAATCTTAGTAGGAACCCTTGGTGTAGCTGGTTACTTGAATATGATTCATCAGATGAAAGAACTAATTACAGCAGCTGGAAAAAAAGTTTATACTCTAGTTATGGGAAGACCGAACCCTGCAAAACTTGCCAACTTTCCAGAGTGTGATATTTTCATTTATGTCTCTTGCGCCCAAACTGCTCTGCTGGATAGCAAAGAATATTTAGCTCCAGTAATCACTCCTTTTGAAGCGATGCTAGCTTTCAGCAGAGGATCTCAGTGGACCGGAGCATATGTAATGGAATTCCGCGATTTGATTAATTCTTCTCCAGTGGACTCGAGAAACCAGTCTGAAGAAGCACGATTTTCCTTCATACAAGGTGGATATGTAGAAGAGTTTGATTGGGAAGGGAATGATGCTGATGAAGACAAGGAAGGAGCTCTTGCTTTAGCAAATGCGACAGAGAAGGCTCTGCAATTGCGTGACAACCCTAATTCCATAGCCAAGCGAACGGTGAAGTCTGGTGCAGAGTTTTTAGCAGCTCGATCCTATCAGGGTCTAGATATACATTATGACACTTCCTTGCCAGGACCATATCTAATGGGGAGGAGTGGGAGGGCAGCAGGGTATCAAGATGAAAAAAGCATGCAAGAGAAATTGGAATGA

Protein Analysis

494

Amino Acids

54.38

Weight (kDa)

5.07

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthamide_syn PF01866 28 - 371 1.9e-80 Putative diphthamide synthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 269, 434
Acc16I TGCGCA 1 cut(s) 334
Acc36I ACCTGC 2 cut(s) 269, 434
AccI GTMKAC 1 cut(s) 228
AccII CGCG 1 cut(s) 1128
AciI CCGC 4 cut(s) 38, 176, 793, 1126
AclWI GGATC 6 cut(s) 444, 457, 509, 674, 1099, 1359
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 783, 1121
AcuI CTGAAG 3 cut(s) 46, 119, 1191
AcyI GRCGYC 1 cut(s) 231
AflII CTTAAG 1 cut(s) 788
AgsI TTSAA 3 cut(s) 473, 877, 1067
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 1 cut(s) 1405
Alw21I GWGCWC 2 cut(s) 127, 1261
Alw26I GTCTC 3 cut(s) 529, 774, 1006
AlwI GGATC 6 cut(s) 444, 457, 509, 674, 1099, 1359
AlwNI CAGNNNCTG 1 cut(s) 914
Ama87I CYCGRG 1 cut(s) 1156
AoxI GGCC 3 cut(s) 132, 183, 253
ApeKI GCWGC 5 cut(s) 440, 545, 911, 1358, 1439
ApoI RAATTY 2 cut(s) 783, 1121
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AseI ATTAAT 1 cut(s) 1137
AspLEI GCGC 2 cut(s) 335, 1010
AspS9I GGNCC 3 cut(s) 132, 1101, 1409
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 3 cut(s) 121, 751, 1345
AsuNHI GCTAGC 1 cut(s) 1075
AvaI CYCGRG 1 cut(s) 1156
AvaII GGWCC 2 cut(s) 1101, 1409
AxyI CCTNAGG 1 cut(s) 630
BalI TGGCCA 1 cut(s) 255
BamHI GGATCC 1 cut(s) 449
BanII GRGCYC 1 cut(s) 1261
BbsI GAAGAC 2 cut(s) 952, 1251
Bbv12I GWGCWC 2 cut(s) 127, 1261
BbvCI CCTCAGC 1 cut(s) 266
BbvI GCAGC 5 cut(s) 452, 557, 923, 1370, 1451
BccI CCATC 2 cut(s) 514, 545
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 1407
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 3 cut(s) 529, 774, 1006
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 4 cut(s) 561, 935, 1076, 1379
BfrI CTTAAG 1 cut(s) 788
BfuAI ACCTGC 2 cut(s) 269, 434
BisI GCNGC 5 cut(s) 441, 546, 912, 1359, 1440
BlsI GCNGC 5 cut(s) 442, 547, 913, 1360, 1441
Bme1390I CCNGG 2 cut(s) 86, 1407
Bme18I GGWCC 2 cut(s) 1101, 1409
BmeT110I CYCGRG 1 cut(s) 1156
BmgT120I GGNCC 3 cut(s) 132, 1101, 1409
BmiI GGNNCC 4 cut(s) 312, 451, 673, 853
BmrFI CCNGG 2 cut(s) 86, 1407
BmsI GCATC 6 cut(s) 227, 727, 749, 814, 1062, 1225
BmtI GCTAGC 1 cut(s) 1079
BpiI GAAGAC 2 cut(s) 952, 1251
BpmI CTGGAG 2 cut(s) 1032, 1131
Bpu10I CCTNAGC 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 86
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 856
BsaWI WCCGGW 1 cut(s) 1103
BsaXI ACNNNNNCTCC 4 cut(s) 600, 630, 1098, 1128
Bse1I ACTGG 3 cut(s) 1049, 1148, 1165
Bse21I CCTNAGG 1 cut(s) 630
BseBI CCWGG 1 cut(s) 1407
BseDI CCNNGG 1 cut(s) 856
BseGI GGATG 2 cut(s) 94, 829
BseMII CTCAG 5 cut(s) 119, 280, 621, 696, 1109
BseNI ACTGG 3 cut(s) 1049, 1148, 1165
BseRI GAGGAG 1 cut(s) 1441
BseXI GCAGC 5 cut(s) 452, 557, 923, 1370, 1451
BsgI GTGCAG 2 cut(s) 769, 1365
Bsh1236I CGCG 1 cut(s) 1128
BshFI GGCC 3 cut(s) 134, 185, 255
BsiHKAI GWGCWC 2 cut(s) 127, 1261
BsiHKCI CYCGRG 1 cut(s) 1156
BsiSI CCGG 3 cut(s) 86, 287, 1104
BslFI GGGAC 2 cut(s) 592, 648
BsmAI GTCTC 3 cut(s) 529, 774, 1006
BsmFI GGGAC 2 cut(s) 592, 648
BsnI GGCC 3 cut(s) 134, 185, 255
BsoBI CYCGRG 1 cut(s) 1156
Bsp1286I GDGCHC 2 cut(s) 127, 1261
Bsp143I GATC 5 cut(s) 449, 514, 666, 1091, 1364
BspACI CCGC 4 cut(s) 38, 176, 793, 1126
BspANI GGCC 3 cut(s) 134, 185, 255
BspCNI CTCAG 5 cut(s) 120, 279, 622, 695, 1108
BspFNI CGCG 1 cut(s) 1128
BspLI GGNNCC 4 cut(s) 312, 451, 673, 853
BspMI ACCTGC 2 cut(s) 269, 434
BspOI GCTAGC 1 cut(s) 1079
BspPI GGATC 6 cut(s) 444, 457, 509, 674, 1099, 1359
BspTI CTTAAG 1 cut(s) 788
BsrI ACTGG 3 cut(s) 1049, 1148, 1165
BssECI CCNNGG 1 cut(s) 856
BssMI GATC 5 cut(s) 449, 514, 666, 1091, 1364
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 1 cut(s) 856
Bst2UI CCWGG 1 cut(s) 1407
Bst4CI ACNGT 7 cut(s) 81, 191, 322, 433, 572, 583, 1333
Bst6I CTCTTC 1 cut(s) 1206
BstACI GRCGYC 1 cut(s) 231
BstAFI CTTAAG 1 cut(s) 788
BstAPI GCANNNNNTGC 2 cut(s) 824, 965
BstC8I GCNNGC 3 cut(s) 461, 1077, 1466
BstDEI CTNAG 6 cut(s) 128, 266, 630, 682, 844, 1095
BstEII GGTNACC 1 cut(s) 757
BstF5I GGATG 2 cut(s) 94, 829
BstFNI CGCG 1 cut(s) 1128
BstHHI GCGC 2 cut(s) 335, 1010
BstKTI GATC 5 cut(s) 452, 517, 669, 1094, 1367
BstMAI GTCTC 3 cut(s) 529, 774, 1006
BstMBI GATC 5 cut(s) 449, 514, 666, 1091, 1364
BstMWI GCNNNNNNNGC 7 cut(s) 131, 182, 261, 824, 965, 1016, 1076
BstNI CCWGG 1 cut(s) 1407
BstNSI RCATGY 2 cut(s) 227, 1468
BstPI GGTNACC 1 cut(s) 757
BstSCI CCNGG 2 cut(s) 84, 1405
BstUI CGCG 1 cut(s) 1128
BstV1I GCAGC 5 cut(s) 452, 557, 923, 1370, 1451
BstV2I GAAGAC 2 cut(s) 952, 1251
BstX2I RGATCY 3 cut(s) 449, 514, 1091
BstYI RGATCY 3 cut(s) 449, 514, 1091
Bsu36I CCTNAGG 1 cut(s) 630
BsuRI GGCC 3 cut(s) 134, 185, 255
BtgZI GCGATG 2 cut(s) 204, 1085
BtsCI GGATG 2 cut(s) 94, 829
BtsIMutI CAGTG 3 cut(s) 318, 1103, 1155
BveI ACCTGC 2 cut(s) 269, 434
Cac8I GCNNGC 3 cut(s) 461, 1077, 1466
CaiI CAGNNNCTG 1 cut(s) 914
CfoI GCGC 2 cut(s) 335, 1010
Cfr13I GGNCC 3 cut(s) 132, 1101, 1409
CseI GACGC 2 cut(s) 239, 645
CviAII CATG 3 cut(s) 224, 436, 1465
DdeI CTNAG 6 cut(s) 128, 266, 630, 682, 844, 1095
DpnI GATC 5 cut(s) 451, 516, 668, 1093, 1366
DpnII GATC 5 cut(s) 449, 514, 666, 1091, 1364
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 1206
EarI CTCTTC 1 cut(s) 1206
EciI GGCGGA 1 cut(s) 191
Ecl136II GAGCTC 1 cut(s) 1259
Eco130I CCWWGG 1 cut(s) 856
Eco24I GRGCYC 1 cut(s) 1261
Eco47I GGWCC 2 cut(s) 1101, 1409
Eco53kI GAGCTC 1 cut(s) 1259
Eco57I CTGAAG 3 cut(s) 46, 119, 1191
Eco81I CCTNAGG 1 cut(s) 630
Eco88I CYCGRG 1 cut(s) 1156
Eco91I GGTNACC 1 cut(s) 757
EcoICRI GAGCTC 1 cut(s) 1259
EcoO109I RGGNCCY 1 cut(s) 132
EcoO65I GGTNACC 1 cut(s) 757
EcoRI GAATTC 2 cut(s) 783, 1121
EcoRII CCWGG 1 cut(s) 1405
EcoT14I CCWWGG 1 cut(s) 856
EcoT22I ATGCAT 1 cut(s) 742
EcoT38I GRGCYC 1 cut(s) 1261
ErhI CCWWGG 1 cut(s) 856
FaeI CATG 3 cut(s) 227, 439, 1468
FalI AAGNNNNNCTT 2 cut(s) 1245, 1277
FaqI GGGAC 2 cut(s) 592, 648
FatI CATG 3 cut(s) 223, 435, 1464
FauNDI CATATG 1 cut(s) 1111
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 5 cut(s) 441, 546, 912, 1359, 1440
FokI GGATG 2 cut(s) 101, 836
FriOI GRGCYC 1 cut(s) 1261
Fsp4HI GCNGC 5 cut(s) 441, 546, 912, 1359, 1440
FspBI CTAG 4 cut(s) 561, 935, 1076, 1379
FspI TGCGCA 1 cut(s) 334
GlaI GCGC 2 cut(s) 334, 1009
GluI GCNGC 5 cut(s) 441, 546, 912, 1359, 1440
GsuI CTGGAG 2 cut(s) 1032, 1131
HaeIII GGCC 3 cut(s) 134, 185, 255
HapII CCGG 3 cut(s) 86, 287, 1104
HgaI GACGC 2 cut(s) 239, 645
HhaI GCGC 2 cut(s) 335, 1010
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 3 cut(s) 227, 439, 1468
Hin6I GCGC 2 cut(s) 333, 1008
HinP1I GCGC 2 cut(s) 333, 1008
HincII GTYRAC 2 cut(s) 229, 705
HindII GTYRAC 2 cut(s) 229, 705
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 5 cut(s) 11, 104, 502, 883, 1154
HpaI GTTAAC 1 cut(s) 705
HpaII CCGG 3 cut(s) 86, 287, 1104
HphI GGTGA 3 cut(s) 121, 751, 1345
Hpy166II GTNNAC 5 cut(s) 205, 229, 705, 1101, 1153
Hpy188I TCNGA 9 cut(s) 99, 129, 328, 454, 676, 731, 745, 891, 1171
Hpy188III TCNNGA 6 cut(s) 64, 664, 977, 1158, 1379, 1451
Hpy8I GTNNAC 5 cut(s) 205, 229, 705, 1101, 1153
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 6 cut(s) 94, 301, 1198, 1220, 1247, 1279
HpyCH4III ACNGT 7 cut(s) 81, 191, 322, 433, 572, 583, 1333
HpyCH4V TGCA 9 cut(s) 459, 692, 740, 750, 839, 959, 1294, 1346, 1468
HpyF10VI GCNNNNNNNGC 7 cut(s) 131, 182, 261, 824, 965, 1016, 1076
HpyF3I CTNAG 6 cut(s) 128, 266, 630, 682, 844, 1095
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 3 cut(s) 227, 439, 1468
HspAI GCGC 2 cut(s) 333, 1008
KspAI GTTAAC 1 cut(s) 705
Kzo9I GATC 5 cut(s) 449, 514, 666, 1091, 1364
LmnI GCTCC 6 cut(s) 215, 316, 542, 1051, 1106, 1256
Lsp1109I GCAGC 5 cut(s) 452, 557, 923, 1370, 1451
LweI GCATC 6 cut(s) 227, 727, 749, 814, 1062, 1225
MaeI CTAG 4 cut(s) 561, 935, 1076, 1379
MaeIII GTNAC 6 cut(s) 17, 345, 566, 757, 869, 1301
MalI GATC 5 cut(s) 451, 516, 668, 1093, 1366
MboI GATC 5 cut(s) 449, 514, 666, 1091, 1364
MboII GAAGA 6 cut(s) 792, 957, 1134, 1184, 1223, 1256
MfeI CAATTG 1 cut(s) 1295
MflI RGATCY 3 cut(s) 449, 514, 1091
MhlI GDGCHC 2 cut(s) 127, 1261
MlsI TGGCCA 1 cut(s) 255
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 2 cut(s) 113, 1148
MmeI TCCRAC 2 cut(s) 193, 375
Mox20I TGGCCA 1 cut(s) 255
Mph1103I ATGCAT 1 cut(s) 742
MscI TGGCCA 1 cut(s) 255
MseI TTAA 3 cut(s) 704, 789, 1137
MslI CAYNNNNRTG 1 cut(s) 745
Msp20I TGGCCA 1 cut(s) 255
MspA1I CMGCKG 3 cut(s) 38, 548, 914
MspCI CTTAAG 1 cut(s) 788
MspI CCGG 3 cut(s) 86, 287, 1104
MspR9I CCNGG 2 cut(s) 86, 1407
MunI CAATTG 1 cut(s) 1295
MvaI CCWGG 1 cut(s) 1407
MvnI CGCG 1 cut(s) 1128
MwoI GCNNNNNNNGC 7 cut(s) 131, 182, 261, 824, 965, 1016, 1076
NciI CCSGG 1 cut(s) 86
NdeI CATATG 1 cut(s) 1111
NdeII GATC 5 cut(s) 449, 514, 666, 1091, 1364
NheI GCTAGC 1 cut(s) 1075
NlaIII CATG 3 cut(s) 227, 439, 1468
NlaIV GGNNCC 4 cut(s) 312, 451, 673, 853
NmuCI GTSAC 3 cut(s) 345, 757, 1301
NsbI TGCGCA 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 742
NspI RCATGY 2 cut(s) 227, 1468
PaeI GCATGC 1 cut(s) 1468
PaeR7I CTCGAG 1 cut(s) 1156
PaqCI CACCTGC 2 cut(s) 269, 434
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 3 cut(s) 11, 502, 883
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 5 cut(s) 442, 547, 913, 1360, 1441
PleI GAGTC 2 cut(s) 112, 1148
PpsI GAGTC 2 cut(s) 112, 1148
PshBI ATTAAT 1 cut(s) 1137
Psp124BI GAGCTC 1 cut(s) 1261
Psp6I CCWGG 1 cut(s) 1405
PspEI GGTNACC 1 cut(s) 757
PspGI CCWGG 1 cut(s) 1405
PspN4I GGNNCC 4 cut(s) 312, 451, 673, 853
PspPI GGNCC 3 cut(s) 132, 1101, 1409
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 3 cut(s) 449, 514, 1091
PvuII CAGCTG 2 cut(s) 548, 914
RseI CAYNNNNRTG 1 cut(s) 745
SacI GAGCTC 1 cut(s) 1261
SalI GTCGAC 1 cut(s) 227
SaqAI TTAA 3 cut(s) 704, 789, 1137
SatI GCNGC 5 cut(s) 441, 546, 912, 1359, 1440
Sau3AI GATC 5 cut(s) 449, 514, 666, 1091, 1364
Sau96I GGNCC 3 cut(s) 132, 1101, 1409
SchI GAGTC 2 cut(s) 113, 1148
ScrFI CCNGG 2 cut(s) 86, 1407
SduI GDGCHC 2 cut(s) 127, 1261
SfaNI GCATC 6 cut(s) 227, 727, 749, 814, 1062, 1225
Sfr274I CTCGAG 1 cut(s) 1156
SinI GGWCC 2 cut(s) 1101, 1409
SlaI CTCGAG 1 cut(s) 1156
SmiMI CAYNNNNRTG 1 cut(s) 745
SmlI CTYRAG 3 cut(s) 519, 788, 1156
SmoI CTYRAG 3 cut(s) 519, 788, 1156
SpeI ACTAGT 1 cut(s) 560
SphI GCATGC 1 cut(s) 1468
SsiI CCGC 4 cut(s) 38, 176, 793, 1126
SspI AATATT 1 cut(s) 1040
SspMI CTAG 4 cut(s) 561, 935, 1076, 1379
SstI GAGCTC 1 cut(s) 1261
StyD4I CCNGG 2 cut(s) 84, 1405
StyI CCWWGG 1 cut(s) 856
TaaI ACNGT 7 cut(s) 81, 191, 322, 433, 572, 583, 1333
TaqI TCGA 3 cut(s) 228, 1157, 1363
TaqII GACCGA 1 cut(s) 964
TfiI GAWTC 3 cut(s) 11, 502, 883
Tru1I TTAA 3 cut(s) 704, 789, 1137
Tru9I TTAA 3 cut(s) 704, 789, 1137
TscAI CASTG 3 cut(s) 325, 1103, 1155
TseFI GTSAC 3 cut(s) 345, 757, 1301
TseI GCWGC 5 cut(s) 440, 545, 911, 1358, 1439
Tsp45I GTSAC 3 cut(s) 345, 757, 1301
TspRI CASTG 3 cut(s) 325, 1103, 1155
Vha464I CTTAAG 1 cut(s) 788
VpaK11BI GGWCC 2 cut(s) 1101, 1409
VspI ATTAAT 1 cut(s) 1137
XapI RAATTY 2 cut(s) 783, 1121
XbaI TCTAGA 1 cut(s) 1378
XceI RCATGY 2 cut(s) 227, 1468
XhoI CTCGAG 1 cut(s) 1156
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 4 cut(s) 561, 935, 1076, 1379
Zsp2I ATGCAT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.