AT3G60790

F-box protein At1g67390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0017910
Physical Location & Seq
Forward (+)
22464623 .. 22466948
2326 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60790.1

Sequence Viewer

Length: 1467 bp
ATGACGACACAATCATCATCATCATCATCATCATTGCCCTCGTCACTATCATCAACACCACCACTATTAGCATCAAATGCAAGATGCAAAGTTTTAAGGACAGGAGCATCATCTAAAGGAAAAGGAAAAGGGATCAAATATCCGGTCGATCGGATAAGCATGCTTCCGGATGAAATGTTACAAAAGATCCTTTCGACACTATCAACTAAAGATGCCGTGATAACAAGCACCTTGTCGAAAAGATGGGTGGATCAGTGGAAGCGCATACCACATCTCTGCGTGGACATGAGAAATATTATGAGAACAAACCCGACCAGCTATGTTCATGAATTATCTTTCCGCTTTGCTGAATCAATGACGAAGACTTTAAACAATCATCGTGGTCATCTAGAGAGCTGCACAATCAGCCATATTCAGTTCATTTTTTTGTTCATCGATAGGTGGATCCAAACAGTGACTCGTGAGAAACAAACCAAGGAGATTACTCTTGTCAACAATATAGGTTGCATGACACCTTTTGTTAGATATAATAGTCTTCACTTGTCCCCAAGTGCATTCTGTCATCCTAGCCTCACTTCACTCTCACTTACTCGATATAAGTTACTCGAGAAAGCCTTCAAGAACTGCTGCAATCTTAAGATCCTGAAGCTTTATGACATCATGTCCGATGTTAGTGTCCTTAGCAACGTTATCAAAGCTTGCTCCTCTCTTGAGGTGCTTGTGTTGCAAATCACATTCTTAAACCAAGCAAGTGCGTTGAAGATCGAAAACAAGAAATTAGAATTCTTGCAAGTGACTTGGCCTTGTCTGATGAATAGGATGGAAGTGAATACACCTCGTTTGGTGATTTTTGACATCAAATCTATCTACTGTTTTGGCTACTCAGTTGAAGCCCCTAAATTAAGCATGTTTAAAAGAGATTATTGGGTTGGTGGGATGAGTTACCCTCATCTTAGCTACCATATATCAAGTCTCGCTCAGGAGAAAATAAGAATTTGGCTTGAGCTTATGGTGAGCCAAATTTATCATATGAAACGGACTGGATCCTTATCCGTGAGTGTCGATGTGAGAAATCCGAACGAAGTCGAGATTTTGAAGGAAGTCTTACTTTTGTGGGATGGAGAGATGATGGATCTGGAGATCCTCTTCAAGAACAACAATGCTCCTATAGAAGAAGGTGAATCTTTTATTACTGGTGGAGCACGTAACAAATGGTGGGATGGTGAAAAACCGTTTCCAGATGATTTCTTCCGCGTTTGTACTGTGTGGATGTATAATTTCGATGGTTCTAATGAAGAGGAATTTGCATTAGCGTCACGTTTTGTGACACAAGGAACAGTGACTGAAAAGTTGATGATAAAGACATCGACGTATCCTCCAGTGAAGCAATTAATGACAGAAGCAAAAGTGGCCAAGCTAATGGAACTTCCTAAAGGTTACGAGTATTTGGACATTGAATGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

488

Amino Acids

55.93

Weight (kDa)

8.83

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 52 - 88 4.4e-07 F-box domain
LRR_At5g56370 PF24758 145 - 282 4.9e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1254
AccIII TCCGGA 1 cut(s) 166
AciI CCGC 2 cut(s) 340, 1252
AclI AACGTT 1 cut(s) 687
AcoI YGGCCR 1 cut(s) 1410
AcsI RAATTY 4 cut(s) 782, 993, 1020, 1301
AcuI CTGAAG 1 cut(s) 665
AfaI GTAC 1 cut(s) 1261
AflII CTTAAG 1 cut(s) 635
AgsI TTSAA 6 cut(s) 619, 760, 890, 1096, 1150, 1457
AhdI GACNNNNNGTC 1 cut(s) 661
AluBI AGCT 7 cut(s) 318, 396, 649, 698, 957, 1006, 1417
AluI AGCT 7 cut(s) 318, 396, 649, 698, 957, 1006, 1417
Alw21I GWGCWC 1 cut(s) 1204
Alw26I GTCTC 1 cut(s) 977
AlwNI CAGNNNCTG 1 cut(s) 1343
Ama87I CYCGRG 1 cut(s) 605
Aor13HI TCCGGA 1 cut(s) 166
AoxI GGCC 2 cut(s) 800, 1410
ApeKI GCWGC 2 cut(s) 396, 627
ApoI RAATTY 4 cut(s) 782, 993, 1020, 1301
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
AseI ATTAAT 1 cut(s) 1391
Asp700I GAANNNNTTC 1 cut(s) 614
AspLEI GCGC 1 cut(s) 264
AsuHPI GGTGA 4 cut(s) 856, 1024, 1190, 1235
AvaI CYCGRG 1 cut(s) 605
BalI TGGCCA 1 cut(s) 1412
BamHI GGATCC 2 cut(s) 444, 1043
BauI CACGAG 1 cut(s) 459
BbsI GAAGAC 2 cut(s) 368, 527
Bbv12I GWGCWC 1 cut(s) 1204
BbvI GCAGC 2 cut(s) 383, 614
BccI CCATC 6 cut(s) 237, 814, 1112, 1123, 1214, 1277
BceAI ACGGC 1 cut(s) 200
BciVI GTATCC 1 cut(s) 1383
BcoDI GTCTC 1 cut(s) 977
BfaI CTAG 2 cut(s) 389, 567
BfmI CTRYAG 1 cut(s) 1167
BfrI CTTAAG 1 cut(s) 635
BfuI GTATCC 1 cut(s) 1383
BisI GCNGC 2 cut(s) 397, 628
BlsI GCNGC 2 cut(s) 398, 629
BmeRI GACNNNNNGTC 1 cut(s) 661
BmeT110I CYCGRG 1 cut(s) 605
BmiI GGNNCC 2 cut(s) 446, 1045
BmsI GCATC 4 cut(s) 74, 80, 116, 202
BpiI GAAGAC 2 cut(s) 368, 527
BplI GAGNNNNNCTC 2 cut(s) 931, 963
BpmI CTGGAG 2 cut(s) 1157, 1362
Bpu10I CCTNAGC 2 cut(s) 680, 978
BpuEI CTTGAG 2 cut(s) 731, 1022
Bsa29I ATCGAT 1 cut(s) 435
BsaAI YACGTR 1 cut(s) 1205
BsaBI GATNNNNATC 1 cut(s) 1048
BsaJI CCNNGG 1 cut(s) 474
BsaWI WCCGGW 2 cut(s) 142, 166
BsaXI ACNNNNNCTCC 2 cut(s) 1360, 1390
Bse1I ACTGG 3 cut(s) 1045, 1198, 1379
Bse3DI GCAATG 1 cut(s) 32
Bse8I GATNNNNATC 1 cut(s) 1048
BseAI TCCGGA 1 cut(s) 166
BseCI ATCGAT 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 474
BseGI GGATG 7 cut(s) 175, 562, 825, 942, 1123, 1225, 1275
BseJI GATNNNNATC 1 cut(s) 1048
BseMI GCAATG 1 cut(s) 32
BseMII CTCAG 2 cut(s) 897, 992
BseNI ACTGG 3 cut(s) 1045, 1198, 1379
BseRI GAGGAG 1 cut(s) 694
BseXI GCAGC 2 cut(s) 383, 614
BsgI GTGCAG 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 1254
Bsh1285I CGRYCG 2 cut(s) 147, 151
BshFI GGCC 2 cut(s) 802, 1412
BshVI ATCGAT 1 cut(s) 435
BsiEI CGRYCG 2 cut(s) 147, 151
BsiHKAI GWGCWC 1 cut(s) 1204
BsiHKCI CYCGRG 1 cut(s) 605
BsiSI CCGG 2 cut(s) 143, 167
BslFI GGGAC 1 cut(s) 529
BsmAI GTCTC 1 cut(s) 977
BsmFI GGGAC 1 cut(s) 529
BsmI GAATGC 2 cut(s) 554, 1463
BsnI GGCC 2 cut(s) 802, 1412
BsoBI CYCGRG 1 cut(s) 605
Bsp1286I GDGCHC 1 cut(s) 1204
Bsp13I TCCGGA 1 cut(s) 166
BspACI CCGC 2 cut(s) 340, 1252
BspANI GGCC 2 cut(s) 802, 1412
BspCNI CTCAG 2 cut(s) 896, 991
BspDI ATCGAT 1 cut(s) 435
BspEI TCCGGA 1 cut(s) 166
BspFNI CGCG 1 cut(s) 1254
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 2 cut(s) 446, 1045
BspTI CTTAAG 1 cut(s) 635
BsrDI GCAATG 1 cut(s) 32
BsrI ACTGG 3 cut(s) 1045, 1198, 1379
BssECI CCNNGG 1 cut(s) 474
BssSI CACGAG 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 459
Bst4CI ACNGT 5 cut(s) 454, 872, 1233, 1264, 1339
Bst6I CTCTTC 2 cut(s) 1151, 1290
BstAFI CTTAAG 1 cut(s) 635
BstAPI GCANNNNNTGC 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 1205
BstC8I GCNNGC 2 cut(s) 161, 700
BstDEI CTNAG 4 cut(s) 680, 883, 953, 978
BstF5I GGATG 7 cut(s) 175, 562, 825, 942, 1123, 1225, 1275
BstFNI CGCG 1 cut(s) 1254
BstHHI GCGC 1 cut(s) 264
BstMAI GTCTC 1 cut(s) 977
BstMCI CGRYCG 2 cut(s) 147, 151
BstMWI GCNNNNNNNGC 4 cut(s) 77, 405, 724, 1409
BstNSI RCATGY 2 cut(s) 163, 910
BstSFI CTRYAG 1 cut(s) 1167
BstUI CGCG 1 cut(s) 1254
BstV1I GCAGC 2 cut(s) 383, 614
BstV2I GAAGAC 2 cut(s) 368, 527
BstX2I RGATCY 6 cut(s) 186, 444, 639, 1043, 1132, 1140
BstXI CCANNNNNNTGG 1 cut(s) 1420
BstYI RGATCY 6 cut(s) 186, 444, 639, 1043, 1132, 1140
Bsu15I ATCGAT 1 cut(s) 435
BsuI GTATCC 1 cut(s) 1383
BsuRI GGCC 2 cut(s) 802, 1412
BsuTUI ATCGAT 1 cut(s) 435
BtsCI GGATG 7 cut(s) 175, 562, 825, 942, 1123, 1225, 1275
BtsIMutI CAGTG 4 cut(s) 260, 459, 1344, 1386
Cac8I GCNNGC 2 cut(s) 161, 700
CaiI CAGNNNCTG 1 cut(s) 1343
CciI TCATGA 1 cut(s) 325
CfoI GCGC 1 cut(s) 264
ClaI ATCGAT 1 cut(s) 435
CseI GACGC 1 cut(s) 1302
Csp6I GTAC 1 cut(s) 1260
CspCI CAANNNNNGTGG 2 cut(s) 361, 396
CviAII CATG 6 cut(s) 160, 286, 326, 508, 661, 907
CviQI GTAC 1 cut(s) 1260
DdeI CTNAG 4 cut(s) 680, 883, 953, 978
DraI TTTAAA 2 cut(s) 369, 913
DriI GACNNNNNGTC 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 1410
Eam1104I CTCTTC 2 cut(s) 1151, 1290
Eam1105I GACNNNNNGTC 1 cut(s) 661
EarI CTCTTC 2 cut(s) 1151, 1290
Eco130I CCWWGG 1 cut(s) 474
Eco57I CTGAAG 1 cut(s) 665
Eco88I CYCGRG 1 cut(s) 605
EcoRI GAATTC 1 cut(s) 782
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 6 cut(s) 163, 289, 329, 511, 664, 910
FalI AAGNNNNNCTT 4 cut(s) 1088, 1120, 1092, 1124
FaqI GGGAC 1 cut(s) 529
FatI CATG 6 cut(s) 159, 285, 325, 507, 660, 906
FauNDI CATATG 1 cut(s) 1029
Fnu4HI GCNGC 2 cut(s) 397, 628
FokI GGATG 7 cut(s) 182, 549, 832, 949, 1130, 1232, 1282
Fsp4HI GCNGC 2 cut(s) 397, 628
FspBI CTAG 2 cut(s) 389, 567
GlaI GCGC 1 cut(s) 263
GluI GCNGC 2 cut(s) 397, 628
GsuI CTGGAG 2 cut(s) 1157, 1362
HaeIII GGCC 2 cut(s) 802, 1412
HapII CCGG 2 cut(s) 143, 167
HgaI GACGC 1 cut(s) 1302
HhaI GCGC 1 cut(s) 264
Hin1II CATG 6 cut(s) 163, 289, 329, 511, 664, 910
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HindIII AAGCTT 2 cut(s) 647, 696
HinfI GANTC 3 cut(s) 350, 457, 1181
HpaII CCGG 2 cut(s) 143, 167
HphI GGTGA 4 cut(s) 856, 1024, 1190, 1235
Hpy166II GTNNAC 2 cut(s) 283, 493
Hpy188I TCNGA 4 cut(s) 153, 667, 810, 1077
Hpy8I GTNNAC 2 cut(s) 283, 493
Hpy99I CGWCG 1 cut(s) 1372
HpyAV CCTTC 3 cut(s) 625, 1090, 1169
HpyCH4III ACNGT 5 cut(s) 454, 872, 1233, 1264, 1339
HpyCH4IV ACGT 4 cut(s) 687, 1204, 1318, 1370
HpyCH4V TGCA 9 cut(s) 80, 87, 399, 507, 554, 630, 727, 790, 1307
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 405, 724, 1409
HpyF3I CTNAG 4 cut(s) 680, 883, 953, 978
HpySE526I ACGT 4 cut(s) 687, 1204, 1318, 1370
Hsp92II CATG 6 cut(s) 163, 289, 329, 511, 664, 910
HspAI GCGC 1 cut(s) 262
Kpn2I TCCGGA 1 cut(s) 166
LmnI GCTCC 4 cut(s) 104, 707, 1168, 1199
Lsp1109I GCAGC 2 cut(s) 383, 614
LweI GCATC 4 cut(s) 74, 80, 116, 202
MaeI CTAG 2 cut(s) 389, 567
MaeII ACGT 4 cut(s) 687, 1204, 1318, 1370
MboII GAAGA 7 cut(s) 373, 527, 772, 1138, 1184, 1240, 1307
MflI RGATCY 6 cut(s) 186, 444, 639, 1043, 1132, 1140
MhlI GDGCHC 1 cut(s) 1204
MlsI TGGCCA 1 cut(s) 1412
MluCI AATT 9 cut(s) 329, 776, 782, 899, 993, 1020, 1276, 1301, 1388
MluNI TGGCCA 1 cut(s) 1412
MlyI GAGTC 1 cut(s) 451
MnlI CCTC 9 cut(s) 49, 581, 706, 715, 846, 957, 1154, 1291, 1386
Mox20I TGGCCA 1 cut(s) 1412
MroI TCCGGA 1 cut(s) 166
MroXI GAANNNNTTC 1 cut(s) 614
MscI TGGCCA 1 cut(s) 1412
MseI TTAA 7 cut(s) 95, 368, 636, 740, 902, 912, 1391
Msp20I TGGCCA 1 cut(s) 1412
MspCI CTTAAG 1 cut(s) 635
MspI CCGG 2 cut(s) 143, 167
Mva1269I GAATGC 2 cut(s) 554, 1463
MvnI CGCG 1 cut(s) 1254
MwoI GCNNNNNNNGC 4 cut(s) 77, 405, 724, 1409
NdeI CATATG 1 cut(s) 1029
NlaIII CATG 6 cut(s) 163, 289, 329, 511, 664, 910
NlaIV GGNNCC 2 cut(s) 446, 1045
NmuCI GTSAC 6 cut(s) 42, 454, 793, 1314, 1324, 1339
NspI RCATGY 2 cut(s) 163, 910
PaeI GCATGC 1 cut(s) 163
PaeR7I CTCGAG 1 cut(s) 605
PagI TCATGA 1 cut(s) 325
PctI GAATGC 2 cut(s) 554, 1463
PdmI GAANNNNTTC 1 cut(s) 614
PfeI GAWTC 2 cut(s) 350, 1181
PkrI GCNGC 2 cut(s) 398, 629
Ple19I CGATCG 1 cut(s) 151
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
Ppu21I YACGTR 1 cut(s) 1205
PshBI ATTAAT 1 cut(s) 1391
Psp1406I AACGTT 1 cut(s) 687
PspN4I GGNNCC 2 cut(s) 446, 1045
PstNI CAGNNNCTG 1 cut(s) 1343
PsuI RGATCY 6 cut(s) 186, 444, 639, 1043, 1132, 1140
PvuI CGATCG 1 cut(s) 151
RsaI GTAC 1 cut(s) 1261
RsaNI GTAC 1 cut(s) 1260
SaqAI TTAA 7 cut(s) 95, 368, 636, 740, 902, 912, 1391
SatI GCNGC 2 cut(s) 397, 628
SchI GAGTC 1 cut(s) 451
SduI GDGCHC 1 cut(s) 1204
SfaNI GCATC 4 cut(s) 74, 80, 116, 202
SfcI CTRYAG 1 cut(s) 1167
Sfr274I CTCGAG 1 cut(s) 605
SlaI CTCGAG 1 cut(s) 605
SmlI CTYRAG 4 cut(s) 605, 635, 710, 1001
SmoI CTYRAG 4 cut(s) 605, 635, 710, 1001
SphI GCATGC 1 cut(s) 163
Sse9I AATT 9 cut(s) 329, 776, 782, 899, 993, 1020, 1276, 1301, 1388
SsiI CCGC 2 cut(s) 340, 1252
SspI AATATT 1 cut(s) 295
SspMI CTAG 2 cut(s) 389, 567
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 5 cut(s) 454, 872, 1233, 1264, 1339
TaiI ACGT 4 cut(s) 690, 1207, 1321, 1373
TasI AATT 9 cut(s) 329, 776, 782, 899, 993, 1020, 1276, 1301, 1388
TatI WGTACW 1 cut(s) 1259
TfiI GAWTC 2 cut(s) 350, 1181
Tru1I TTAA 7 cut(s) 95, 368, 636, 740, 902, 912, 1391
Tru9I TTAA 7 cut(s) 95, 368, 636, 740, 902, 912, 1391
TscAI CASTG 4 cut(s) 260, 459, 1344, 1386
TseFI GTSAC 6 cut(s) 42, 454, 793, 1314, 1324, 1339
TseI GCWGC 2 cut(s) 396, 627
Tsp45I GTSAC 6 cut(s) 42, 454, 793, 1314, 1324, 1339
TspDTI ATGAA 8 cut(s) 186, 314, 342, 409, 421, 827, 1046, 1308
TspGWI ACGGA 2 cut(s) 1042, 1051
TspRI CASTG 4 cut(s) 260, 459, 1344, 1386
Vha464I CTTAAG 1 cut(s) 635
VspI ATTAAT 1 cut(s) 1391
XapI RAATTY 4 cut(s) 782, 993, 1020, 1301
XbaI TCTAGA 1 cut(s) 388
XceI RCATGY 2 cut(s) 163, 910
XhoI CTCGAG 1 cut(s) 605
XmnI GAANNNNTTC 1 cut(s) 614
XspI CTAG 2 cut(s) 389, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.