AT3G63200

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015100
Physical Location & Seq
Forward (+)
23345754 .. 23347672
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G63200.1

Sequence Viewer

Length: 1155 bp
ATGGAGATGGATCTCAGCAAGGTTACTCTTGACATTTTCACAAAGCTTGAACAGAAATGGCTCTCTCACTGCGACTCCTCCCGGAAGACTCGTATCCTTAGCATCGACGGTGGCGGTACCACTGGCATTGTCGCCGCTGCTTCTATTCTCCACCTCGAGCACCAGATCCGCCTTCAAACCGGCGACCCTCACGCTCACATCTCTGATTTCTTCGACATTGTCGCCGGAACTGGCATCGGAGGCATTCTCGCCGCCCTCCTTGTCGCCGACGACGGCTCCGGCAGGCCGATGTTCACTGCCAGAGACGCGGTTAAGTTTGTCGCGGAGAAGAACTCGGAGCTCTTCGAGATCAGGTACACCGGAGTTTTCAGGAGGAACAAGAGATACTCGGGGAAGAGCATGGAGAGGGTTTTGGAGACGGCGTTTAGGAGAGAGGACGGTAAGGTGTTGACGATGAAGGACACGTGTAAGCCTCTCCTTGTTCCTTGCTACGACCTCAAAACCTCTGCTCCTTTCGTTTTCTCACGCGCCGGCGCGTCTGAGTCTCCGAGCTTCGACTTCGAGCTGTGGAAAGTCTGCCGTGCCACGTCAGCAACACCAAGCCTCTTCAAGCCGTTCAGTGTAGTGTCGGTGGACGGGAAAACCTCATGCTCAGCCGTAGACGGCGGTTTGGTGATGAACAATCCAACAGCAGCTGCCGTCACGCACGTGCTACACAACAAACGAGATTTCCCGTCAGTAAACGGCGTAGATGACTTGCTTGTACTGTCGTTGGGAAACGGTCCGTCGACCATGTCATCATCACCAGGGAGGAAACTCCGTCGTAACGGAGACTATTCAACGTCAAGTGTGGTGGACATAGTGGTTGACGGCGTTTCCGATACCGTCGATCAGATGCTGGGGAACGCTTTCTGCTGGAACCGTACTGATTACGTTAGAATCCAGGCGAACGGTTTGACGAGCGGCGGAGCGGAGGAGTTGCTGAAAGAGAGAGGTGTGGAAACGGCGCCGTTTGGGGTAAAACGGATACTAACGGAGAGTAACGGAGAAAGAATAGAGGGTTTCGTGCAACGTCTTGTTGCGTCAGGAAAGTCAAGTCTACCTCCAAGTCCTTGCAAGGAATCTGCCGTTAACCCTCTCGCTGACGGCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

384

Amino Acids

41.49

Weight (kDa)

7.61

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 33 - 233 1.8e-21 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63200
fragaria_vesca FvH4_2g23520 FvH4_2g23520
malus_domestica MD03G1264100.v1.1 MD11G1284700.v1.1
prunus_persica Prupe.8G240600_v2.0.a1
pyrus_communis pycom03g21180
rosa_chinensis RchiOBHm_Chr6g0290991
rosa_laevigata RLG00000012157
rosa_roxburghii Rroxscaffold_7G00176480
rosa_rugosa Rorug06G0217000
rosa_samantha Rh6AG326700 Rh6BG334200 Rh6CG340400 Rh6DG327600
rosa_wichuraiana Rw6G028410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 116
AccB1I GGYRCC 2 cut(s) 116, 1006
AccBSI CCGCTC 2 cut(s) 963, 971
AccI GTMKAC 3 cut(s) 660, 788, 1099
AccII CGCG 4 cut(s) 308, 323, 528, 536
AclWI GGATC 2 cut(s) 18, 160
AcoI YGGCCR 1 cut(s) 1147
AcvI CACGTG 2 cut(s) 465, 709
AcyI GRCGYC 1 cut(s) 1007
AfaI GTAC 4 cut(s) 118, 356, 765, 925
AflIII ACRYGT 2 cut(s) 462, 464
AgsI TTSAA 4 cut(s) 50, 176, 610, 840
AjiI CACGTC 1 cut(s) 588
AjnI CCWGG 2 cut(s) 805, 942
AleI CACNNNNGTG 1 cut(s) 707
AluBI AGCT 5 cut(s) 46, 340, 552, 565, 695
AluI AGCT 5 cut(s) 46, 340, 552, 565, 695
Alw21I GWGCWC 2 cut(s) 162, 342
Alw26I GTCTC 4 cut(s) 297, 410, 549, 825
AlwI GGATC 2 cut(s) 18, 160
AlwNI CAGNNNCTG 2 cut(s) 695, 898
Ama87I CYCGRG 2 cut(s) 155, 388
AoxI GGCC 2 cut(s) 284, 1147
ApeKI GCWGC 3 cut(s) 137, 692, 695
Asp700I GAANNNNTTC 1 cut(s) 908
Asp718I GGTACC 1 cut(s) 116
AspLEI GCGC 3 cut(s) 530, 536, 1009
AspS9I GGNCC 1 cut(s) 782
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 685, 795
AvaI CYCGRG 2 cut(s) 155, 388
AvaII GGWCC 1 cut(s) 782
BanI GGYRCC 2 cut(s) 116, 1006
BanII GRGCYC 1 cut(s) 342
BbrPI CACGTG 2 cut(s) 465, 709
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 2 cut(s) 162, 342
BbvI GCAGC 3 cut(s) 124, 682, 704
BciT130I CCWGG 2 cut(s) 807, 944
BciVI GTATCC 2 cut(s) 104, 1020
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 4 cut(s) 297, 410, 549, 825
BfoI RGCGCY 1 cut(s) 1010
BfuI GTATCC 2 cut(s) 104, 1020
BisI GCNGC 6 cut(s) 135, 138, 252, 693, 696, 964
BlpI GCTNAGC 1 cut(s) 652
BlsI GCNGC 6 cut(s) 136, 139, 253, 694, 697, 965
Bme1390I CCNGG 3 cut(s) 82, 807, 944
Bme18I GGWCC 1 cut(s) 782
BmeT110I CYCGRG 2 cut(s) 155, 388
BmgBI CACGTC 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 782
BmiI GGNNCC 4 cut(s) 118, 277, 920, 1008
BmrFI CCNGG 3 cut(s) 82, 807, 944
BmsI GCATC 3 cut(s) 111, 243, 885
BpiI GAAGAC 1 cut(s) 92
BplI GAGNNNNNCTC 4 cut(s) 231, 263, 317, 349
Bpu10I CCTNAGC 1 cut(s) 98
Bpu1102I GCTNAGC 1 cut(s) 652
BpuMI CCSGG 1 cut(s) 82
BsaAI YACGTR 2 cut(s) 465, 709
BsaHI GRCGYC 1 cut(s) 1007
BsaJI CCNNGG 1 cut(s) 806
BsaWI WCCGGW 1 cut(s) 359
BsaXI ACNNNNNCTCC 8 cut(s) 59, 89, 260, 290, 407, 437, 493, 523
Bse118I RCCGGY 2 cut(s) 179, 530
Bse1I ACTGG 2 cut(s) 127, 235
BseBI CCWGG 2 cut(s) 807, 944
BseDI CCNNGG 1 cut(s) 806
BseMII CTCAG 3 cut(s) 28, 531, 666
BseNI ACTGG 2 cut(s) 127, 235
BseRI GAGGAG 2 cut(s) 67, 989
BseX3I CGGCCG 1 cut(s) 1147
BseXI GCAGC 3 cut(s) 124, 682, 704
BseYI CCCAGC 1 cut(s) 898
Bsh1236I CGCG 4 cut(s) 308, 323, 528, 536
Bsh1285I CGRYCG 1 cut(s) 1150
BshFI GGCC 2 cut(s) 286, 1149
BshNI GGYRCC 2 cut(s) 116, 1006
BsiEI CGRYCG 1 cut(s) 1150
BsiHKAI GWGCWC 2 cut(s) 162, 342
BsiHKCI CYCGRG 2 cut(s) 155, 388
BsiSI CCGG 6 cut(s) 82, 180, 225, 279, 360, 531
BsmAI GTCTC 4 cut(s) 297, 410, 549, 825
BsmBI CGTCTC 2 cut(s) 297, 410
BsmI GAATGC 1 cut(s) 243
BsnI GGCC 2 cut(s) 286, 1149
BsoBI CYCGRG 2 cut(s) 155, 388
Bsp1286I GDGCHC 2 cut(s) 162, 342
Bsp143I GATC 4 cut(s) 10, 165, 348, 889
Bsp1720I GCTNAGC 1 cut(s) 652
BspANI GGCC 2 cut(s) 286, 1149
BspCNI CTCAG 3 cut(s) 27, 532, 665
BspFNI CGCG 4 cut(s) 308, 323, 528, 536
BspLI GGNNCC 4 cut(s) 118, 277, 920, 1008
BspPI GGATC 2 cut(s) 18, 160
BspQI GCTCTTC 2 cut(s) 347, 389
BspT107I GGYRCC 2 cut(s) 116, 1006
BsrBI CCGCTC 2 cut(s) 963, 971
BsrFI RCCGGY 2 cut(s) 179, 530
BsrI ACTGG 2 cut(s) 127, 235
BssAI RCCGGY 2 cut(s) 179, 530
BssECI CCNNGG 1 cut(s) 806
BssMI GATC 4 cut(s) 10, 165, 348, 889
BssNI GRCGYC 1 cut(s) 1007
Bst2UI CCWGG 2 cut(s) 807, 944
Bst4CI ACNGT 7 cut(s) 110, 440, 768, 782, 886, 923, 953
Bst6I CTCTTC 3 cut(s) 347, 389, 611
BstACI GRCGYC 1 cut(s) 1007
BstBAI YACGTR 2 cut(s) 465, 709
BstC8I GCNNGC 2 cut(s) 284, 532
BstDEI CTNAG 4 cut(s) 14, 98, 540, 652
BstFNI CGCG 4 cut(s) 308, 323, 528, 536
BstH2I RGCGCY 1 cut(s) 1010
BstHHI GCGC 3 cut(s) 530, 536, 1009
BstKTI GATC 4 cut(s) 13, 168, 351, 892
BstMAI GTCTC 4 cut(s) 297, 410, 549, 825
BstMBI GATC 4 cut(s) 10, 165, 348, 889
BstMCI CGRYCG 1 cut(s) 1150
BstMWI GCNNNNNNNGC 3 cut(s) 240, 305, 590
BstNI CCWGG 2 cut(s) 807, 944
BstSCI CCNGG 3 cut(s) 80, 805, 942
BstUI CGCG 4 cut(s) 308, 323, 528, 536
BstV1I GCAGC 3 cut(s) 124, 682, 704
BstV2I GAAGAC 1 cut(s) 92
BstX2I RGATCY 2 cut(s) 10, 165
BstYI RGATCY 2 cut(s) 10, 165
BstZI CGGCCG 1 cut(s) 1147
BsuI GTATCC 2 cut(s) 104, 1020
BsuRI GGCC 2 cut(s) 286, 1149
BtrI CACGTC 1 cut(s) 588
BtsI GCAGTG 2 cut(s) 67, 294
BtsIMutI CAGTG 4 cut(s) 67, 120, 294, 625
Cac8I GCNNGC 2 cut(s) 284, 532
CaiI CAGNNNCTG 2 cut(s) 695, 898
CfoI GCGC 3 cut(s) 530, 536, 1009
Cfr10I RCCGGY 2 cut(s) 179, 530
Cfr13I GGNCC 1 cut(s) 782
CpoI CGGWCCG 1 cut(s) 782
CseI GACGC 3 cut(s) 314, 525, 1071
Csp6I GTAC 4 cut(s) 117, 355, 764, 924
CspCI CAANNNNNGTGG 4 cut(s) 109, 144, 834, 869
CspI CGGWCCG 1 cut(s) 782
CviAII CATG 3 cut(s) 400, 648, 793
CviQI GTAC 4 cut(s) 117, 355, 764, 924
DdeI CTNAG 4 cut(s) 14, 98, 540, 652
DinI GGCGCC 1 cut(s) 1008
DpnI GATC 4 cut(s) 12, 167, 350, 891
DpnII GATC 4 cut(s) 10, 165, 348, 889
EaeI YGGCCR 1 cut(s) 1147
EagI CGGCCG 1 cut(s) 1147
Eam1104I CTCTTC 3 cut(s) 347, 389, 611
EarI CTCTTC 3 cut(s) 347, 389, 611
EciI GGCGGA 2 cut(s) 158, 981
Ecl136II GAGCTC 1 cut(s) 340
EclXI CGGCCG 1 cut(s) 1147
Eco24I GRGCYC 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 782
Eco52I CGGCCG 1 cut(s) 1147
Eco53kI GAGCTC 1 cut(s) 340
Eco72I CACGTG 2 cut(s) 465, 709
Eco88I CYCGRG 2 cut(s) 155, 388
EcoICRI GAGCTC 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 805, 942
EcoT38I GRGCYC 1 cut(s) 342
EgeI GGCGCC 1 cut(s) 1008
EheI GGCGCC 1 cut(s) 1008
Esp3I CGTCTC 2 cut(s) 297, 410
FaeI CATG 3 cut(s) 403, 651, 796
FaiI YATR 4 cut(s) 401, 649, 794, 860
FatI CATG 3 cut(s) 399, 647, 792
FblI GTMKAC 3 cut(s) 660, 788, 1099
Fnu4HI GCNGC 6 cut(s) 135, 138, 252, 693, 696, 964
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 6 cut(s) 135, 138, 252, 693, 696, 964
GlaI GCGC 3 cut(s) 529, 535, 1008
GluI GCNGC 6 cut(s) 135, 138, 252, 693, 696, 964
GsaI CCCAGC 1 cut(s) 902
HaeII RGCGCY 1 cut(s) 1010
HaeIII GGCC 2 cut(s) 286, 1149
HapII CCGG 6 cut(s) 82, 180, 225, 279, 360, 531
HgaI GACGC 3 cut(s) 314, 525, 1071
HhaI GCGC 3 cut(s) 530, 536, 1009
Hin1I GRCGYC 1 cut(s) 1007
Hin1II CATG 3 cut(s) 403, 651, 796
Hin6I GCGC 3 cut(s) 528, 534, 1007
HinP1I GCGC 3 cut(s) 528, 534, 1007
HincII GTYRAC 4 cut(s) 450, 789, 868, 1132
HindII GTYRAC 4 cut(s) 450, 789, 868, 1132
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 5 cut(s) 74, 88, 542, 939, 1121
HpaI GTTAAC 1 cut(s) 1132
HpaII CCGG 6 cut(s) 82, 180, 225, 279, 360, 531
HphI GGTGA 2 cut(s) 685, 795
Hpy188I TCNGA 7 cut(s) 205, 239, 337, 541, 549, 880, 894
Hpy188III TCNNGA 4 cut(s) 29, 346, 370, 1086
Hpy99I CGWCG 6 cut(s) 110, 272, 275, 790, 825, 890
HpyAV CCTTC 2 cut(s) 182, 451
HpyCH4III ACNGT 7 cut(s) 110, 440, 768, 782, 886, 923, 953
HpyCH4IV ACGT 6 cut(s) 464, 587, 708, 842, 933, 1072
HpyCH4V TGCA 2 cut(s) 1069, 1116
HpyF10VI GCNNNNNNNGC 3 cut(s) 240, 305, 590
HpyF3I CTNAG 4 cut(s) 14, 98, 540, 652
HpySE526I ACGT 6 cut(s) 464, 587, 708, 842, 933, 1072
Hsp92I GRCGYC 1 cut(s) 1007
Hsp92II CATG 3 cut(s) 403, 651, 796
HspAI GCGC 3 cut(s) 528, 534, 1007
KasI GGCGCC 1 cut(s) 1006
KpnI GGTACC 1 cut(s) 120
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
KspAI GTTAAC 1 cut(s) 1132
Kzo9I GATC 4 cut(s) 10, 165, 348, 889
LguI GCTCTTC 2 cut(s) 347, 389
LmnI GCTCC 4 cut(s) 281, 337, 514, 968
Lsp1109I GCAGC 3 cut(s) 124, 682, 704
LweI GCATC 3 cut(s) 111, 243, 885
MaeII ACGT 6 cut(s) 464, 587, 708, 842, 933, 1072
MaeIII GTNAC 4 cut(s) 22, 700, 824, 1040
MalI GATC 4 cut(s) 12, 167, 350, 891
MbiI CCGCTC 2 cut(s) 963, 971
MboI GATC 4 cut(s) 10, 165, 348, 889
MboII GAAGA 6 cut(s) 97, 202, 334, 340, 406, 598
MflI RGATCY 2 cut(s) 10, 165
MhlI GDGCHC 2 cut(s) 162, 342
Mly113I GGCGCC 1 cut(s) 1007
MlyI GAGTC 3 cut(s) 68, 82, 551
MmeI TCCRAC 1 cut(s) 710
MreI CGCCGGCG 1 cut(s) 530
MroNI GCCGGC 1 cut(s) 530
MroXI GAANNNNTTC 1 cut(s) 908
MseI TTAA 3 cut(s) 312, 1131, 1153
MslI CAYNNNNRTG 1 cut(s) 707
MspA1I CMGCKG 2 cut(s) 137, 695
MspI CCGG 6 cut(s) 82, 180, 225, 279, 360, 531
MspR9I CCNGG 3 cut(s) 82, 807, 944
Mva1269I GAATGC 1 cut(s) 243
MvaI CCWGG 2 cut(s) 807, 944
MvnI CGCG 4 cut(s) 308, 323, 528, 536
MwoI GCNNNNNNNGC 3 cut(s) 240, 305, 590
NaeI GCCGGC 1 cut(s) 532
NarI GGCGCC 1 cut(s) 1007
NciI CCSGG 1 cut(s) 82
NdeII GATC 4 cut(s) 10, 165, 348, 889
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 3 cut(s) 403, 651, 796
NlaIV GGNNCC 4 cut(s) 118, 277, 920, 1008
NmuCI GTSAC 1 cut(s) 700
OliI CACNNNNGTG 1 cut(s) 707
PaeR7I CTCGAG 1 cut(s) 155
PciSI GCTCTTC 2 cut(s) 347, 389
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PctI GAATGC 1 cut(s) 243
PdiI GCCGGC 1 cut(s) 532
PdmI GAANNNNTTC 1 cut(s) 908
PfeI GAWTC 2 cut(s) 939, 1121
PflFI GACNNNGTC 2 cut(s) 218, 793
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 6 cut(s) 136, 139, 253, 694, 697, 965
PleI GAGTC 3 cut(s) 68, 82, 550
PluTI GGCGCC 1 cut(s) 1010
PmaCI CACGTG 2 cut(s) 465, 709
PmlI CACGTG 2 cut(s) 465, 709
PpsI GAGTC 3 cut(s) 68, 82, 550
Ppu21I YACGTR 2 cut(s) 465, 709
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 805, 942
PspCI CACGTG 2 cut(s) 465, 709
PspFI CCCAGC 1 cut(s) 898
PspGI CCWGG 2 cut(s) 805, 942
PspN4I GGNNCC 4 cut(s) 118, 277, 920, 1008
PspPI GGNCC 1 cut(s) 782
PspXI VCTCGAGB 1 cut(s) 155
PsrI GAACNNNNNNTAC 2 cut(s) 368, 400
PstNI CAGNNNCTG 2 cut(s) 695, 898
PsuI RGATCY 2 cut(s) 10, 165
PsyI GACNNNGTC 2 cut(s) 218, 793
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 4 cut(s) 118, 356, 765, 925
RsaNI GTAC 4 cut(s) 117, 355, 764, 924
RseI CAYNNNNRTG 1 cut(s) 707
Rsr2I CGGWCCG 1 cut(s) 782
RsrII CGGWCCG 1 cut(s) 782
SacI GAGCTC 1 cut(s) 342
SalI GTCGAC 1 cut(s) 787
SapI GCTCTTC 2 cut(s) 347, 389
SaqAI TTAA 3 cut(s) 312, 1131, 1153
SatI GCNGC 6 cut(s) 135, 138, 252, 693, 696, 964
Sau3AI GATC 4 cut(s) 10, 165, 348, 889
Sau96I GGNCC 1 cut(s) 782
SchI GAGTC 3 cut(s) 68, 82, 551
ScrFI CCNGG 3 cut(s) 82, 807, 944
SduI GDGCHC 2 cut(s) 162, 342
SfaNI GCATC 3 cut(s) 111, 243, 885
SfoI GGCGCC 1 cut(s) 1008
Sfr274I CTCGAG 1 cut(s) 155
SgrAI CRCCGGYG 1 cut(s) 530
SinI GGWCC 1 cut(s) 782
SlaI CTCGAG 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
SspDI GGCGCC 1 cut(s) 1006
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 3 cut(s) 80, 805, 942
TaaI ACNGT 7 cut(s) 110, 440, 768, 782, 886, 923, 953
TaiI ACGT 6 cut(s) 467, 590, 711, 845, 936, 1075
TaqI TCGA 8 cut(s) 105, 156, 213, 345, 555, 561, 788, 888
TatI WGTACW 1 cut(s) 763
TauI GCSGC 3 cut(s) 137, 254, 966
TfiI GAWTC 2 cut(s) 939, 1121
Tru1I TTAA 3 cut(s) 312, 1131, 1153
Tru9I TTAA 3 cut(s) 312, 1131, 1153
TscAI CASTG 4 cut(s) 74, 127, 301, 625
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 3 cut(s) 137, 692, 695
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 2 cut(s) 470, 692
TspGWI ACGGA 6 cut(s) 774, 809, 843, 1039, 1049, 1059
TspRI CASTG 4 cut(s) 74, 127, 301, 625
Tth111I GACNNNGTC 2 cut(s) 218, 793
VpaK11BI GGWCC 1 cut(s) 782
XhoI CTCGAG 1 cut(s) 155
XmiI GTMKAC 3 cut(s) 660, 788, 1099
XmnI GAANNNNTTC 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.