pycom03g21180

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015100
Physical Location & Seq
Forward (+)
21476920 .. 21478164
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21180.2

Sequence Viewer

Length: 1161 bp
ATGGAGCTGAGCAAGGTAACCTTTGAGATCGTCACGAAGCTGGAGCAGAAGTGGCTCTCCCACTGCGAAACCGCCAAGAAGACTCGGATCCTCAGCATCGACGGCGGCGGAACCACCGGCATTGTCGCCGGAACCGCCTTGATCCACCTCGAGGATCAGATCCGACTCAAAACCGGCGACGCCCACGCTCAAATCGCCGATTTCTTCGACCTCGTCACTGGTACTGGCATCGGAGCCGTCCTGGCCGCCATGCTTGTCGCCGATGACGGCTCCGGCCGCCCTCTCTACACCGCTAGAGATGCTGTGAACTCGGTTTCCGAGAAGAATGCCGACATGTTCAAATTGATGTTTGGTGGAGTGTTTCGCCGGCGCCGGAGGTACTCCGGCGGGAGCATGGAGAAGGCGTTGGCGGAGCTTCTTACGAGGGAGGACGGGAAGGTCCTGACGCTCAAGGACACGTGTAAGCCTCTCCTGGTTCCCTGCTACGACATGAAAAGTTCCGCCCCGTTCGTGTTCTCCCGAGCCGACGCGTCCGAGTCGGCGAGTTTCGACTTCGAAATGTGGAAAGTCTGCCGGGCCACGTCAGCGACTCCAGGCGTGTTCAAGCCGTTCAGCCTGATCTCCGTGGACGGGAAGACATCGTGCTCGGCGGTGGACGGTGGTCTGGTGATGAACAACCCGACGGCGGCTGCTGTGACTCACGTACTCAACAACAAGCGCGACTTCCCCTCCGTCAATGGCGTCGAGGACTTGCTGGTTTTGTCGTTAGGTAACGGCCCGTTGAGCGGAGGGAAGGCGGGGCGGAGTAACGGAAAGTGCTCGCCCTCGTCGGTGGTTGACATTGTGCTGGACGGAGTTTCCGAGACCATTGACCAGATGGTGGGAAACGCCTTCTGTTATAACCGCGCTGATTACGTCAGAATTCAGGCTTTCGGATTGGGGAGCGAAGTGGTTGCGGGCCAGCGATCGAAGGAGGAAGCGGAGGTGTTGAAGGAGAGAGGGGTGGAGTCGTTACCGTTTGGCGGGAAGCGGTTATTGACGGAGACAAACGGAGAGAGAATCGAGGGTTTCGTGCAACGACTCGTGGCTTGTGGAAGGAGCAGTCTGCCACCTAGTCCCCGCAAGGACTCCGGCGTTAGCGCCCTCGCTAACGGCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

387

Amino Acids

41.17

Weight (kDa)

8.05

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63200
fragaria_vesca FvH4_2g23520 FvH4_2g23520
malus_domestica MD03G1264100.v1.1 MD11G1284700.v1.1
prunus_persica Prupe.8G240600_v2.0.a1
pyrus_communis pycom03g21180
rosa_chinensis RchiOBHm_Chr6g0290991
rosa_laevigata RLG00000012157
rosa_roxburghii Rroxscaffold_7G00176480
rosa_rugosa Rorug06G0217000
rosa_samantha Rh6AG326700 Rh6BG334200 Rh6CG340400 Rh6DG327600
rosa_wichuraiana Rw6G028410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 900
AasI GACNNNNNNGTC 1 cut(s) 437
AbsI CCTCGAGG 1 cut(s) 149
AccB1I GGYRCC 1 cut(s) 369
AccB7I CCANNNNNTGG 1 cut(s) 880
AccBSI CCGCTC 1 cut(s) 786
AccII CGCG 3 cut(s) 530, 720, 906
AclWI GGATC 5 cut(s) 82, 95, 136, 154, 162
AcoI YGGCCR 3 cut(s) 243, 274, 1153
AcsI RAATTY 1 cut(s) 921
AcvI CACGTG 1 cut(s) 459
AcyI GRCGYC 3 cut(s) 180, 370, 741
AfaI GTAC 3 cut(s) 223, 380, 705
AfiI CCNNNNNNNGG 6 cut(s) 151, 630, 685, 785, 880, 1022
AflIII ACRYGT 4 cut(s) 333, 456, 458, 528
AgsI TTSAA 3 cut(s) 340, 604, 991
AjiI CACGTC 1 cut(s) 582
AjnI CCWGG 3 cut(s) 240, 471, 592
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
AluBI AGCT 3 cut(s) 7, 40, 415
AluI AGCT 3 cut(s) 7, 40, 415
Alw21I GWGCWC 2 cut(s) 647, 821
Alw26I GTCTC 2 cut(s) 857, 1037
AlwI GGATC 5 cut(s) 82, 95, 136, 154, 162
Ama87I CYCGRG 2 cut(s) 149, 519
AoxI GGCC 6 cut(s) 243, 274, 576, 775, 958, 1153
ApeKI GCWGC 1 cut(s) 689
ApoI RAATTY 1 cut(s) 921
AspLEI GCGC 4 cut(s) 372, 720, 908, 1142
AspS9I GGNCC 4 cut(s) 439, 576, 776, 958
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 1 cut(s) 679
AsuII TTCGAA 1 cut(s) 555
AvaI CYCGRG 2 cut(s) 149, 519
AvaII GGWCC 1 cut(s) 439
BamHI GGATCC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 369
BauI CACGAG 1 cut(s) 1082
BbrPI CACGTG 1 cut(s) 459
BbsI GAAGAC 2 cut(s) 86, 641
Bbv12I GWGCWC 2 cut(s) 647, 821
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 676
BccI CCATC 1 cut(s) 871
BceAI ACGGC 7 cut(s) 118, 221, 283, 592, 699, 790, 1140
BcgI CGANNNNNNTGC 4 cut(s) 308, 342, 935, 969
BciT130I CCWGG 3 cut(s) 242, 473, 594
BcnI CCSGG 1 cut(s) 575
BcoDI GTCTC 2 cut(s) 857, 1037
BfaI CTAG 2 cut(s) 294, 1113
BfoI RGCGCY 2 cut(s) 373, 1143
BglI GCCNNNNNGGC 1 cut(s) 242
BisI GCNGC 5 cut(s) 106, 246, 277, 687, 690
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 5 cut(s) 107, 247, 278, 688, 691
Bme1390I CCNGG 4 cut(s) 242, 473, 575, 594
Bme18I GGWCC 1 cut(s) 439
BmeT110I CYCGRG 2 cut(s) 149, 519
BmgBI CACGTC 1 cut(s) 582
BmgT120I GGNCC 4 cut(s) 439, 576, 776, 958
BmiI GGNNCC 7 cut(s) 89, 112, 133, 235, 271, 371, 477
BmrFI CCNGG 4 cut(s) 242, 473, 575, 594
BmsI GCATC 3 cut(s) 105, 237, 289
BoxI GACNNNNGTC 1 cut(s) 660
BpiI GAAGAC 2 cut(s) 86, 641
BpmI CTGGAG 2 cut(s) 62, 576
Bpu10I CCTNAGC 1 cut(s) 92
Bpu1102I GCTNAGC 1 cut(s) 8
Bpu14I TTCGAA 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 575
BsaAI YACGTR 2 cut(s) 459, 703
BsaHI GRCGYC 3 cut(s) 180, 370, 741
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 1 cut(s) 624
BsaXI ACNNNNNCTCC 6 cut(s) 35, 65, 389, 419, 713, 743
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 630, 685, 785, 880, 1022
Bse118I RCCGGY 3 cut(s) 116, 173, 366
Bse1I ACTGG 2 cut(s) 223, 229
BseBI CCWGG 3 cut(s) 242, 473, 594
BseDI CCNNGG 1 cut(s) 624
BseLI CCNNNNNNNGG 6 cut(s) 151, 630, 685, 785, 880, 1022
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 2 cut(s) 223, 229
BseX3I CGGCCG 2 cut(s) 274, 1153
BseXI GCAGC 1 cut(s) 676
Bsh1236I CGCG 3 cut(s) 530, 720, 906
Bsh1285I CGRYCG 3 cut(s) 277, 968, 1156
BshFI GGCC 6 cut(s) 245, 276, 578, 777, 960, 1155
BshNI GGYRCC 1 cut(s) 369
BsiEI CGRYCG 3 cut(s) 277, 968, 1156
BsiHKAI GWGCWC 2 cut(s) 647, 821
BsiHKCI CYCGRG 2 cut(s) 149, 519
BsiSI CCGG 9 cut(s) 117, 129, 174, 273, 367, 373, 384, 574, 1131
BslFI GGGAC 1 cut(s) 1101
BslI CCNNNNNNNGG 6 cut(s) 151, 630, 685, 785, 880, 1022
BsmAI GTCTC 2 cut(s) 857, 1037
BsmFI GGGAC 1 cut(s) 1101
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 6 cut(s) 245, 276, 578, 777, 960, 1155
Bso31I GGTCTC 1 cut(s) 857
BsoBI CYCGRG 2 cut(s) 149, 519
Bsp119I TTCGAA 1 cut(s) 555
Bsp1286I GDGCHC 2 cut(s) 647, 821
Bsp143I GATC 7 cut(s) 27, 87, 141, 154, 159, 618, 965
Bsp1720I GCTNAGC 1 cut(s) 8
BspANI GGCC 6 cut(s) 245, 276, 578, 777, 960, 1155
BspCNI CTCAG 1 cut(s) 105
BspFNI CGCG 3 cut(s) 530, 720, 906
BspLI GGNNCC 7 cut(s) 89, 112, 133, 235, 271, 371, 477
BspPI GGATC 5 cut(s) 82, 95, 136, 154, 162
BspT104I TTCGAA 1 cut(s) 555
BspT107I GGYRCC 1 cut(s) 369
BspTNI GGTCTC 1 cut(s) 857
BsrBI CCGCTC 1 cut(s) 786
BsrFI RCCGGY 3 cut(s) 116, 173, 366
BsrI ACTGG 2 cut(s) 223, 229
BssAI RCCGGY 3 cut(s) 116, 173, 366
BssECI CCNNGG 1 cut(s) 624
BssMI GATC 7 cut(s) 27, 87, 141, 154, 159, 618, 965
BssNI GRCGYC 3 cut(s) 180, 370, 741
BssSI CACGAG 1 cut(s) 1082
Bst2BI CACGAG 1 cut(s) 1082
Bst2UI CCWGG 3 cut(s) 242, 473, 594
Bst4CI ACNGT 2 cut(s) 659, 1017
BstACI GRCGYC 3 cut(s) 180, 370, 741
BstBAI YACGTR 2 cut(s) 459, 703
BstBI TTCGAA 1 cut(s) 555
BstC8I GCNNGC 4 cut(s) 368, 821, 958, 962
BstDEI CTNAG 2 cut(s) 8, 92
BstDSI CCRYGG 1 cut(s) 624
BstEII GGTNACC 1 cut(s) 16
BstFNI CGCG 3 cut(s) 530, 720, 906
BstH2I RGCGCY 2 cut(s) 373, 1143
BstHHI GCGC 4 cut(s) 372, 720, 908, 1142
BstKTI GATC 7 cut(s) 30, 90, 144, 157, 162, 621, 968
BstMAI GTCTC 2 cut(s) 857, 1037
BstMBI GATC 7 cut(s) 27, 87, 141, 154, 159, 618, 965
BstMCI CGRYCG 3 cut(s) 277, 968, 1156
BstMWI GCNNNNNNNGC 9 cut(s) 52, 102, 134, 194, 242, 276, 299, 584, 783
BstNI CCWGG 3 cut(s) 242, 473, 594
BstNSI RCATGY 1 cut(s) 337
BstPAI GACNNNNGTC 1 cut(s) 660
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 4 cut(s) 240, 471, 573, 592
BstUI CGCG 3 cut(s) 530, 720, 906
BstV1I GCAGC 1 cut(s) 676
BstV2I GAAGAC 2 cut(s) 86, 641
BstX2I RGATCY 2 cut(s) 87, 159
BstYI RGATCY 2 cut(s) 87, 159
BstZI CGGCCG 2 cut(s) 274, 1153
BsuRI GGCC 6 cut(s) 245, 276, 578, 777, 960, 1155
BtgI CCRYGG 1 cut(s) 624
BtrI CACGTC 1 cut(s) 582
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 61, 216
Cac8I GCNNGC 4 cut(s) 368, 821, 958, 962
CfoI GCGC 4 cut(s) 372, 720, 908, 1142
Cfr10I RCCGGY 3 cut(s) 116, 173, 366
Cfr13I GGNCC 4 cut(s) 439, 576, 776, 958
CseI GACGC 5 cut(s) 188, 454, 519, 536, 730
Csp6I GTAC 3 cut(s) 222, 379, 704
CspCI CAANNNNNGTGG 2 cut(s) 103, 138
CviAII CATG 4 cut(s) 250, 334, 394, 490
CviQI GTAC 3 cut(s) 222, 379, 704
DdeI CTNAG 2 cut(s) 8, 92
DinI GGCGCC 1 cut(s) 371
DpnI GATC 7 cut(s) 29, 89, 143, 156, 161, 620, 967
DpnII GATC 7 cut(s) 27, 87, 141, 154, 159, 618, 965
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 3 cut(s) 243, 274, 1153
EagI CGGCCG 2 cut(s) 274, 1153
EciI GGCGGA 4 cut(s) 123, 425, 490, 817
EclXI CGGCCG 2 cut(s) 274, 1153
Eco31I GGTCTC 1 cut(s) 857
Eco47I GGWCC 1 cut(s) 439
Eco52I CGGCCG 2 cut(s) 274, 1153
Eco72I CACGTG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 149, 519
Eco91I GGTNACC 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 439
EcoO65I GGTNACC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 921
EcoRII CCWGG 3 cut(s) 240, 471, 592
EgeI GGCGCC 1 cut(s) 371
EheI GGCGCC 1 cut(s) 371
FaeI CATG 4 cut(s) 253, 337, 397, 493
FaiI YATR 5 cut(s) 251, 335, 395, 491, 900
FalI AAGNNNNNCTT 3 cut(s) 37, 707, 739
FaqI GGGAC 1 cut(s) 1101
FatI CATG 4 cut(s) 249, 333, 393, 489
FauI CCCGC 5 cut(s) 380, 790, 949, 1016, 1127
Fnu4HI GCNGC 5 cut(s) 106, 246, 277, 687, 690
Fsp4HI GCNGC 5 cut(s) 106, 246, 277, 687, 690
FspBI CTAG 2 cut(s) 294, 1113
GlaI GCGC 4 cut(s) 371, 719, 907, 1141
GluI GCNGC 5 cut(s) 106, 246, 277, 687, 690
GsuI CTGGAG 2 cut(s) 62, 576
HaeII RGCGCY 2 cut(s) 373, 1143
HaeIII GGCC 6 cut(s) 245, 276, 578, 777, 960, 1155
HapII CCGG 9 cut(s) 117, 129, 174, 273, 367, 373, 384, 574, 1131
HgaI GACGC 5 cut(s) 188, 454, 519, 536, 730
HhaI GCGC 4 cut(s) 372, 720, 908, 1142
Hin1I GRCGYC 3 cut(s) 180, 370, 741
Hin1II CATG 4 cut(s) 253, 337, 397, 493
Hin6I GCGC 4 cut(s) 370, 718, 906, 1140
HinP1I GCGC 4 cut(s) 370, 718, 906, 1140
HincII GTYRAC 1 cut(s) 838
HindII GTYRAC 1 cut(s) 838
HinfI GANTC 9 cut(s) 82, 165, 536, 589, 697, 1007, 1059, 1080, 1127
HpaII CCGG 9 cut(s) 117, 129, 174, 273, 367, 373, 384, 574, 1131
HphI GGTGA 1 cut(s) 679
Hpy166II GTNNAC 4 cut(s) 307, 628, 655, 838
Hpy188I TCNGA 9 cut(s) 87, 159, 164, 233, 319, 535, 862, 920, 935
Hpy188III TCNNGA 3 cut(s) 34, 442, 519
Hpy8I GTNNAC 4 cut(s) 307, 628, 655, 838
Hpy99I CGWCG 6 cut(s) 104, 182, 530, 685, 746, 832
HpyAV CCTTC 7 cut(s) 394, 430, 787, 901, 964, 985, 1089
HpyCH4III ACNGT 2 cut(s) 659, 1017
HpyCH4IV ACGT 4 cut(s) 458, 581, 702, 915
HpyCH4V TGCA 1 cut(s) 1075
HpyF10VI GCNNNNNNNGC 9 cut(s) 52, 102, 134, 194, 242, 276, 299, 584, 783
HpyF3I CTNAG 2 cut(s) 8, 92
HpySE526I ACGT 4 cut(s) 458, 581, 702, 915
Hsp92I GRCGYC 3 cut(s) 180, 370, 741
Hsp92II CATG 4 cut(s) 253, 337, 397, 493
HspAI GCGC 4 cut(s) 370, 718, 906, 1140
KasI GGCGCC 1 cut(s) 369
KroI GCCGGC 1 cut(s) 366
KroNI GCCGGC 1 cut(s) 368
Kzo9I GATC 7 cut(s) 27, 87, 141, 154, 159, 618, 965
LmnI GCTCC 8 cut(s) 4, 43, 233, 275, 390, 412, 942, 1098
Lsp1109I GCAGC 1 cut(s) 676
LweI GCATC 3 cut(s) 105, 237, 289
MaeI CTAG 2 cut(s) 294, 1113
MaeII ACGT 4 cut(s) 458, 581, 702, 915
MaeIII GTNAC 7 cut(s) 16, 31, 214, 694, 770, 806, 1011
MalI GATC 7 cut(s) 29, 89, 143, 156, 161, 620, 967
MbiI CCGCTC 1 cut(s) 786
MboI GATC 7 cut(s) 27, 87, 141, 154, 159, 618, 965
MboII GAAGA 4 cut(s) 91, 196, 334, 646
MflI RGATCY 2 cut(s) 87, 159
MhlI GDGCHC 2 cut(s) 647, 821
MluCI AATT 2 cut(s) 341, 921
MluI ACGCGT 1 cut(s) 528
Mly113I GGCGCC 1 cut(s) 370
MlyI GAGTC 8 cut(s) 76, 159, 545, 583, 691, 1016, 1074, 1121
MmeI TCCRAC 1 cut(s) 187
MreI CGCCGGCG 1 cut(s) 366
MroNI GCCGGC 1 cut(s) 366
MspI CCGG 9 cut(s) 117, 129, 174, 273, 367, 373, 384, 574, 1131
MspR9I CCNGG 4 cut(s) 242, 473, 575, 594
Mva1269I GAATGC 1 cut(s) 331
MvaI CCWGG 3 cut(s) 242, 473, 594
MvnI CGCG 3 cut(s) 530, 720, 906
MwoI GCNNNNNNNGC 9 cut(s) 52, 102, 134, 194, 242, 276, 299, 584, 783
NaeI GCCGGC 1 cut(s) 368
NarI GGCGCC 1 cut(s) 370
NciI CCSGG 1 cut(s) 575
NdeII GATC 7 cut(s) 27, 87, 141, 154, 159, 618, 965
NgoMIV GCCGGC 1 cut(s) 366
NlaIII CATG 4 cut(s) 253, 337, 397, 493
NlaIV GGNNCC 7 cut(s) 89, 112, 133, 235, 271, 371, 477
NmeAIII GCCGAG 1 cut(s) 626
NmuCI GTSAC 3 cut(s) 31, 214, 694
NspI RCATGY 1 cut(s) 337
NspV TTCGAA 1 cut(s) 555
PaeR7I CTCGAG 1 cut(s) 149
PciI ACATGT 1 cut(s) 333
PcsI WCGNNNNNNNCGW 2 cut(s) 858, 1068
PctI GAATGC 1 cut(s) 331
PdiI GCCGGC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 1059
PflFI GACNNNGTC 1 cut(s) 212
PflMI CCANNNNNTGG 1 cut(s) 880
PkrI GCNGC 5 cut(s) 107, 247, 278, 688, 691
Ple19I CGATCG 1 cut(s) 968
PleI GAGTC 8 cut(s) 76, 159, 544, 583, 691, 1015, 1074, 1121
PluTI GGCGCC 1 cut(s) 373
PmaCI CACGTG 1 cut(s) 459
PmlI CACGTG 1 cut(s) 459
PpsI GAGTC 8 cut(s) 76, 159, 544, 583, 691, 1015, 1074, 1121
Ppu21I YACGTR 2 cut(s) 459, 703
PpuMI RGGWCCY 1 cut(s) 439
PscI ACATGT 1 cut(s) 333
PshAI GACNNNNGTC 1 cut(s) 660
PsiI TTATAA 1 cut(s) 900
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 3 cut(s) 240, 471, 592
PspCI CACGTG 1 cut(s) 459
PspEI GGTNACC 1 cut(s) 16
PspGI CCWGG 3 cut(s) 240, 471, 592
PspN4I GGNNCC 7 cut(s) 89, 112, 133, 235, 271, 371, 477
PspPI GGNCC 4 cut(s) 439, 576, 776, 958
PspPPI RGGWCCY 1 cut(s) 439
PspXI VCTCGAGB 1 cut(s) 149
PsuI RGATCY 2 cut(s) 87, 159
PsyI GACNNNGTC 1 cut(s) 212
PvuI CGATCG 1 cut(s) 968
RsaI GTAC 3 cut(s) 223, 380, 705
RsaNI GTAC 3 cut(s) 222, 379, 704
SatI GCNGC 5 cut(s) 106, 246, 277, 687, 690
Sau3AI GATC 7 cut(s) 27, 87, 141, 154, 159, 618, 965
Sau96I GGNCC 4 cut(s) 439, 576, 776, 958
SchI GAGTC 8 cut(s) 76, 159, 545, 583, 691, 1016, 1074, 1121
ScrFI CCNGG 4 cut(s) 242, 473, 575, 594
SduI GDGCHC 2 cut(s) 647, 821
SfaNI GCATC 3 cut(s) 105, 237, 289
SfoI GGCGCC 1 cut(s) 371
Sfr274I CTCGAG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 555
SgrAI CRCCGGYG 1 cut(s) 366
SinI GGWCC 1 cut(s) 439
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 2 cut(s) 149, 449
SmoI CTYRAG 2 cut(s) 149, 449
Sse9I AATT 2 cut(s) 341, 921
SspDI GGCGCC 1 cut(s) 369
SspMI CTAG 2 cut(s) 294, 1113
StyD4I CCNGG 4 cut(s) 240, 471, 573, 592
TaaI ACNGT 2 cut(s) 659, 1017
TaiI ACGT 4 cut(s) 461, 584, 705, 918
TaqI TCGA 8 cut(s) 99, 150, 207, 549, 555, 744, 968, 1062
TasI AATT 2 cut(s) 341, 921
TauI GCSGC 4 cut(s) 108, 248, 279, 689
TfiI GAWTC 1 cut(s) 1059
TscAI CASTG 2 cut(s) 68, 223
TseFI GTSAC 3 cut(s) 31, 214, 694
TseI GCWGC 1 cut(s) 689
Tsp45I GTSAC 3 cut(s) 31, 214, 694
TspDTI ATGAA 2 cut(s) 506, 686
TspGWI ACGGA 6 cut(s) 613, 721, 825, 867, 1055, 1065
TspRI CASTG 2 cut(s) 68, 223
Tth111I GACNNNGTC 1 cut(s) 212
Van91I CCANNNNNTGG 1 cut(s) 880
VpaK11BI GGWCC 1 cut(s) 439
XapI RAATTY 1 cut(s) 921
XceI RCATGY 1 cut(s) 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 874
XhoI CTCGAG 1 cut(s) 149
XspI CTAG 2 cut(s) 294, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.