Rh6AG326700

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015100
Physical Location & Seq
Forward (+)
52904228 .. 52906611
2384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG326700.1

Sequence Viewer

Length: 1155 bp
ATGGAGCTGAGTAAGGTGACCTTGGAGATCTTCACGAAGCTCGAGCAGCAATGGCTCTCTCACTGCGAGGCCTCCAAGAAGACACGGATTCTCAGCATTGACGGCGGAGGAACCACCGGCATTGTCGCCGGACAAGCCCTTATCCATCTCGAGGACCAGATCCGCCTCAAGACCCGCGATCCCCACGCTCAAATCGCCGATTTCTTCGACCTCGTCGCCGGAACCGGCATCGGGGCCCTCCTCGCGGCCATGCTCGTCGCCGATGACGGCTCCGGCAGGCCTCTGTACACTGCTAGAGATGCTGTAAACTCGGTCGCCGAGAAGAGCGAGAATTTGTTCAAGGCTAGGGTGGTAGGAGTTTTCCGCCGTGGCCGGAGGTACTCCGGCGGAAGCATGGACAAGGCGCTGACGGAGCTGCTGACCAGGGAGGACGGCAAGGTCTTGACTCTCAAGGACACGTGTAAGCCTCTCCTGGTCCCCTGCTACGACTTGAAGAGCTCCGCACCGTTCGTTTTCTCCCGCGCCGACGCTTCCGAGTCGCCGAGTTTCGACTTCGAGCTCCGCAAAGTCTGCCGCGCCACGTCAGCGACGCCGAGCCTGTTCAAGCCGTTCGACCTGAGCTCGGTGGACGGAAAGACGTCGTGCTCGGCCGTGGACGGAGGTCTGGTCATGAACAACCCGACGGCGGCGGCTGTGACGCACGCGCTCAACAACAAGCGTGACTTCCCCTCCGTCAACGGCGTGGAGGACTTGCTCGTTTTTTCCTTGGGGAACGGGCCCTTGAGCGGACGGAAGCCTCGCCGTAACGGCGAGTGCTCTCCCTCTTCCGTTGTTGACATTGTGCTCGACGGCGTTTCCGAGACGGTTGACCAGATGGTGGGGAACGCCTTCTGTTGGAACCGTACGGACTACGTCAGAATTCAGGCCTTTGGATTGGGCAGTGACGGAGATGTGGGGCGGAGATCAGAGCAAGTGGCGGTGTTGAAAGAGAGAGGAGTGGAGACGTTACCGTTTGGCGGGAAGAGGTTACTGACGGAGACAAACGGAGAGAGAATCGAGGGTTTCGTGCAACGACTCGTGGCTTGTGGAAGGAGCAGTTTGCCACCAAGTCCGTGCAAGGATTCGGCCGTTAGGCCTCTCGCTAACGGTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

384

Amino Acids

41.31

Weight (kDa)

8.32

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 31 - 231 5.7e-22 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63200
fragaria_vesca FvH4_2g23520 FvH4_2g23520
malus_domestica MD03G1264100.v1.1 MD11G1284700.v1.1
prunus_persica Prupe.8G240600_v2.0.a1
pyrus_communis pycom03g21180
rosa_chinensis RchiOBHm_Chr6g0290991
rosa_laevigata RLG00000012157
rosa_roxburghii Rroxscaffold_7G00176480
rosa_rugosa Rorug06G0217000
rosa_samantha Rh6AG326700 Rh6BG334200 Rh6CG340400 Rh6DG327600
rosa_wichuraiana Rw6G028410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 437
AatII GACGTC 1 cut(s) 641
AccB7I CCANNNNNTGG 1 cut(s) 877
AccBSI CCGCTC 1 cut(s) 786
AccII CGCG 5 cut(s) 177, 245, 522, 576, 704
AclWI GGATC 2 cut(s) 154, 173
AcoI YGGCCR 4 cut(s) 246, 370, 648, 1125
AcsI RAATTY 2 cut(s) 331, 918
AcvI CACGTG 1 cut(s) 459
AcyI GRCGYC 2 cut(s) 590, 638
AfaI GTAC 3 cut(s) 287, 380, 904
AfiI CCNNNNNNNGG 9 cut(s) 151, 231, 244, 622, 685, 785, 877, 894, 1016
AflIII ACRYGT 2 cut(s) 456, 458
AgsI TTSAA 4 cut(s) 340, 493, 604, 985
AjiI CACGTC 1 cut(s) 582
AjnI CCWGG 2 cut(s) 422, 471
AluBI AGCT 6 cut(s) 7, 40, 415, 498, 559, 621
AluI AGCT 6 cut(s) 7, 40, 415, 498, 559, 621
Alw21I GWGCWC 6 cut(s) 500, 561, 623, 647, 818, 846
Alw26I GTCTC 3 cut(s) 854, 995, 1031
AlwI GGATC 2 cut(s) 154, 173
Ama87I CYCGRG 2 cut(s) 41, 149
ApaI GGGCCC 2 cut(s) 238, 780
ApeKI GCWGC 2 cut(s) 46, 415
ApoI RAATTY 2 cut(s) 331, 918
Asp700I GAANNNNTTC 2 cut(s) 335, 887
AspLEI GCGC 4 cut(s) 406, 524, 578, 706
AspS9I GGNCC 6 cut(s) 154, 234, 235, 475, 776, 777
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 2 cut(s) 41, 149
AvaII GGWCC 2 cut(s) 154, 475
BaeGI GKGCMC 2 cut(s) 238, 780
BanII GRGCYC 5 cut(s) 238, 500, 561, 623, 780
BauI CACGAG 1 cut(s) 1076
BbrPI CACGTG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 6 cut(s) 500, 561, 623, 647, 818, 846
BbvI GCAGC 2 cut(s) 58, 402
BccI CCATC 2 cut(s) 153, 868
BciT130I CCWGG 2 cut(s) 424, 473
BcoDI GTCTC 3 cut(s) 854, 995, 1031
BfaI CTAG 2 cut(s) 294, 345
BfoI RGCGCY 1 cut(s) 407
BglI GCCNNNNNGGC 1 cut(s) 807
BglII AGATCT 1 cut(s) 27
BisI GCNGC 6 cut(s) 47, 246, 416, 574, 687, 690
BlsI GCNGC 6 cut(s) 48, 247, 417, 575, 688, 691
Bme1390I CCNGG 2 cut(s) 424, 473
Bme18I GGWCC 2 cut(s) 154, 475
BmeT110I CYCGRG 2 cut(s) 41, 149
BmgBI CACGTC 1 cut(s) 582
BmgT120I GGNCC 6 cut(s) 154, 234, 235, 475, 776, 777
BmiI GGNNCC 8 cut(s) 112, 223, 235, 236, 271, 477, 778, 899
BmrFI CCNGG 2 cut(s) 424, 473
BmsI GCATC 2 cut(s) 237, 289
BoxI GACNNNNGTC 1 cut(s) 660
BpiI GAAGAC 1 cut(s) 86
Bpu10I CCTNAGC 1 cut(s) 617
BpuEI CTTGAG 3 cut(s) 152, 434, 802
BsaAI YACGTR 1 cut(s) 459
BsaHI GRCGYC 2 cut(s) 590, 638
BsaJI CCNNGG 5 cut(s) 21, 367, 423, 651, 765
BsaXI ACNNNNNCTCC 4 cut(s) 651, 681, 713, 743
Bsc4I CCNNNNNNNGG 9 cut(s) 151, 231, 244, 622, 685, 785, 877, 894, 1016
Bse118I RCCGGY 2 cut(s) 116, 224
Bse3DI GCAATG 1 cut(s) 56
BseBI CCWGG 2 cut(s) 424, 473
BseDI CCNNGG 5 cut(s) 21, 367, 423, 651, 765
BseLI CCNNNNNNNGG 9 cut(s) 151, 231, 244, 622, 685, 785, 877, 894, 1016
BseMI GCAATG 1 cut(s) 56
BseMII CTCAG 2 cut(s) 106, 608
BseRI GAGGAG 2 cut(s) 230, 1008
BseSI GKGCMC 2 cut(s) 238, 780
BseX3I CGGCCG 2 cut(s) 648, 1125
BseXI GCAGC 2 cut(s) 58, 402
Bsh1236I CGCG 5 cut(s) 177, 245, 522, 576, 704
Bsh1285I CGRYCG 4 cut(s) 315, 651, 1128, 1150
BsiEI CGRYCG 4 cut(s) 315, 651, 1128, 1150
BsiHKAI GWGCWC 6 cut(s) 500, 561, 623, 647, 818, 846
BsiHKCI CYCGRG 2 cut(s) 41, 149
BsiSI CCGG 7 cut(s) 117, 129, 219, 225, 273, 373, 384
BsiWI CGTACG 1 cut(s) 902
BslFI GGGAC 1 cut(s) 461
BslI CCNNNNNNNGG 9 cut(s) 151, 231, 244, 622, 685, 785, 877, 894, 1016
BsmAI GTCTC 3 cut(s) 854, 995, 1031
BsmBI CGTCTC 2 cut(s) 854, 995
BsmFI GGGAC 1 cut(s) 461
BsoBI CYCGRG 2 cut(s) 41, 149
Bsp120I GGGCCC 2 cut(s) 234, 776
Bsp1286I GDGCHC 8 cut(s) 238, 500, 561, 623, 647, 780, 818, 846
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 4 cut(s) 27, 159, 178, 962
BspCNI CTCAG 2 cut(s) 105, 609
BspFNI CGCG 5 cut(s) 177, 245, 522, 576, 704
BspHI TCATGA 1 cut(s) 669
BspLI GGNNCC 8 cut(s) 112, 223, 235, 236, 271, 477, 778, 899
BspPI GGATC 2 cut(s) 154, 173
BspQI GCTCTTC 2 cut(s) 317, 488
BsrBI CCGCTC 1 cut(s) 786
BsrDI GCAATG 1 cut(s) 56
BsrFI RCCGGY 2 cut(s) 116, 224
BsrGI TGTACA 1 cut(s) 285
BssAI RCCGGY 2 cut(s) 116, 224
BssECI CCNNGG 5 cut(s) 21, 367, 423, 651, 765
BssMI GATC 4 cut(s) 27, 159, 178, 962
BssNI GRCGYC 2 cut(s) 590, 638
BssSI CACGAG 1 cut(s) 1076
BssT1I CCWWGG 2 cut(s) 21, 765
Bst2BI CACGAG 1 cut(s) 1076
Bst2UI CCWGG 2 cut(s) 424, 473
Bst4CI ACNGT 5 cut(s) 507, 865, 902, 1011, 1148
Bst6I CTCTTC 4 cut(s) 317, 488, 829, 1016
BstACI GRCGYC 2 cut(s) 590, 638
BstAPI GCANNNNNTGC 1 cut(s) 570
BstAUI TGTACA 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 459
BstC8I GCNNGC 2 cut(s) 278, 702
BstDEI CTNAG 3 cut(s) 8, 92, 617
BstDSI CCRYGG 2 cut(s) 367, 651
BstEII GGTNACC 1 cut(s) 16
BstFNI CGCG 5 cut(s) 177, 245, 522, 576, 704
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 4 cut(s) 406, 524, 578, 706
BstKTI GATC 4 cut(s) 30, 162, 181, 965
BstMAI GTCTC 3 cut(s) 854, 995, 1031
BstMBI GATC 4 cut(s) 27, 159, 178, 962
BstMCI CGRYCG 4 cut(s) 315, 651, 1128, 1150
BstNI CCWGG 2 cut(s) 424, 473
BstPAI GACNNNNGTC 1 cut(s) 660
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 2 cut(s) 422, 471
BstSLI GKGCMC 2 cut(s) 238, 780
BstUI CGCG 5 cut(s) 177, 245, 522, 576, 704
BstV1I GCAGC 2 cut(s) 58, 402
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 2 cut(s) 27, 159
BstYI RGATCY 2 cut(s) 27, 159
BstZI CGGCCG 2 cut(s) 648, 1125
BtgI CCRYGG 2 cut(s) 367, 651
BtrI CACGTC 1 cut(s) 582
BtsI GCAGTG 3 cut(s) 61, 288, 946
BtsIMutI CAGTG 3 cut(s) 61, 288, 946
Cac8I GCNNGC 2 cut(s) 278, 702
CciI TCATGA 1 cut(s) 669
CfoI GCGC 4 cut(s) 406, 524, 578, 706
Cfr10I RCCGGY 2 cut(s) 116, 224
Cfr13I GGNCC 6 cut(s) 154, 234, 235, 475, 776, 777
CseI GACGC 3 cut(s) 536, 598, 706
Csp6I GTAC 3 cut(s) 286, 379, 903
CspCI CAANNNNNGTGG 2 cut(s) 103, 138
CviAII CATG 3 cut(s) 250, 394, 670
CviQI GTAC 3 cut(s) 286, 379, 903
DdeI CTNAG 3 cut(s) 8, 92, 617
DpnI GATC 4 cut(s) 29, 161, 180, 964
DpnII GATC 4 cut(s) 27, 159, 178, 962
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 4 cut(s) 246, 370, 648, 1125
EagI CGGCCG 2 cut(s) 648, 1125
Eam1104I CTCTTC 4 cut(s) 317, 488, 829, 1016
EarI CTCTTC 4 cut(s) 317, 488, 829, 1016
EciI GGCGGA 5 cut(s) 120, 152, 353, 402, 973
Ecl136II GAGCTC 3 cut(s) 498, 559, 621
EclXI CGGCCG 2 cut(s) 648, 1125
Eco130I CCWWGG 2 cut(s) 21, 765
Eco147I AGGCCT 4 cut(s) 71, 280, 926, 1135
Eco24I GRGCYC 5 cut(s) 238, 500, 561, 623, 780
Eco47I GGWCC 2 cut(s) 154, 475
Eco52I CGGCCG 2 cut(s) 648, 1125
Eco53kI GAGCTC 3 cut(s) 498, 559, 621
Eco72I CACGTG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 41, 149
Eco91I GGTNACC 1 cut(s) 16
EcoICRI GAGCTC 3 cut(s) 498, 559, 621
EcoO109I RGGNCCY 3 cut(s) 234, 235, 777
EcoO65I GGTNACC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 918
EcoRII CCWGG 2 cut(s) 422, 471
EcoT14I CCWWGG 2 cut(s) 21, 765
EcoT38I GRGCYC 5 cut(s) 238, 500, 561, 623, 780
ErhI CCWWGG 2 cut(s) 21, 765
Esp3I CGTCTC 2 cut(s) 854, 995
FaeI CATG 3 cut(s) 253, 397, 673
FaiI YATR 3 cut(s) 251, 395, 671
FalI AAGNNNNNCTT 3 cut(s) 37, 707, 739
FaqI GGGAC 1 cut(s) 461
FatI CATG 3 cut(s) 249, 393, 669
FauI CCCGC 3 cut(s) 182, 527, 1010
Fnu4HI GCNGC 6 cut(s) 47, 246, 416, 574, 687, 690
FriOI GRGCYC 5 cut(s) 238, 500, 561, 623, 780
Fsp4HI GCNGC 6 cut(s) 47, 246, 416, 574, 687, 690
FspBI CTAG 2 cut(s) 294, 345
GlaI GCGC 4 cut(s) 405, 523, 577, 705
GluI GCNGC 6 cut(s) 47, 246, 416, 574, 687, 690
HaeII RGCGCY 1 cut(s) 407
HapII CCGG 7 cut(s) 117, 129, 219, 225, 273, 373, 384
HgaI GACGC 3 cut(s) 536, 598, 706
HhaI GCGC 4 cut(s) 406, 524, 578, 706
Hin1I GRCGYC 2 cut(s) 590, 638
Hin1II CATG 3 cut(s) 253, 397, 673
Hin6I GCGC 4 cut(s) 404, 522, 576, 704
HinP1I GCGC 4 cut(s) 404, 522, 576, 704
HincII GTYRAC 3 cut(s) 736, 835, 868
HindII GTYRAC 3 cut(s) 736, 835, 868
HinfI GANTC 6 cut(s) 88, 445, 536, 1053, 1074, 1121
HpaII CCGG 7 cut(s) 117, 129, 219, 225, 273, 373, 384
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 7 cut(s) 288, 307, 628, 655, 736, 835, 868
Hpy188I TCNGA 4 cut(s) 535, 859, 917, 967
Hpy188III TCNNGA 5 cut(s) 34, 149, 169, 442, 670
Hpy8I GTNNAC 7 cut(s) 288, 307, 628, 655, 736, 835, 868
Hpy99I CGWCG 7 cut(s) 218, 260, 530, 592, 643, 685, 851
HpyAV CCTTC 2 cut(s) 898, 1083
HpyCH4III ACNGT 5 cut(s) 507, 865, 902, 1011, 1148
HpyCH4IV ACGT 5 cut(s) 458, 581, 638, 912, 1004
HpyCH4V TGCA 2 cut(s) 1069, 1116
HpyF3I CTNAG 3 cut(s) 8, 92, 617
HpySE526I ACGT 5 cut(s) 458, 581, 638, 912, 1004
Hsp92I GRCGYC 2 cut(s) 590, 638
Hsp92II CATG 3 cut(s) 253, 397, 673
HspAI GCGC 4 cut(s) 404, 522, 576, 704
Kzo9I GATC 4 cut(s) 27, 159, 178, 962
LguI GCTCTTC 2 cut(s) 317, 488
LmnI GCTCC 6 cut(s) 4, 275, 412, 503, 564, 1092
Lsp1109I GCAGC 2 cut(s) 58, 402
LweI GCATC 2 cut(s) 237, 289
MaeI CTAG 2 cut(s) 294, 345
MaeII ACGT 5 cut(s) 458, 581, 638, 912, 1004
MaeIII GTNAC 7 cut(s) 16, 694, 719, 803, 941, 1005, 1026
MalI GATC 4 cut(s) 29, 161, 180, 964
MbiI CCGCTC 1 cut(s) 786
MboI GATC 4 cut(s) 27, 159, 178, 962
MboII GAAGA 7 cut(s) 22, 91, 196, 334, 505, 816, 1033
MflI RGATCY 2 cut(s) 27, 159
MhlI GDGCHC 8 cut(s) 238, 500, 561, 623, 647, 780, 818, 846
MluCI AATT 2 cut(s) 331, 918
MlyI GAGTC 3 cut(s) 439, 545, 1068
MmeI TCCRAC 1 cut(s) 875
MroXI GAANNNNTTC 2 cut(s) 335, 887
MspI CCGG 7 cut(s) 117, 129, 219, 225, 273, 373, 384
MspR9I CCNGG 2 cut(s) 424, 473
MvaI CCWGG 2 cut(s) 424, 473
MvnI CGCG 5 cut(s) 177, 245, 522, 576, 704
NdeII GATC 4 cut(s) 27, 159, 178, 962
NlaIII CATG 3 cut(s) 253, 397, 673
NlaIV GGNNCC 8 cut(s) 112, 223, 235, 236, 271, 477, 778, 899
NmeAIII GCCGAG 4 cut(s) 343, 567, 618, 626
NmuCI GTSAC 4 cut(s) 16, 694, 719, 941
PaeR7I CTCGAG 2 cut(s) 41, 149
PagI TCATGA 1 cut(s) 669
PceI AGGCCT 4 cut(s) 71, 280, 926, 1135
PciSI GCTCTTC 2 cut(s) 317, 488
PcsI WCGNNNNNNNCGW 2 cut(s) 855, 1062
PdmI GAANNNNTTC 2 cut(s) 335, 887
PfeI GAWTC 3 cut(s) 88, 1053, 1121
Pfl23II CGTACG 1 cut(s) 902
PflFI GACNNNGTC 2 cut(s) 212, 911
PflMI CCANNNNNTGG 1 cut(s) 877
PkrI GCNGC 6 cut(s) 48, 247, 417, 575, 688, 691
PleI GAGTC 3 cut(s) 439, 544, 1068
PmaCI CACGTG 1 cut(s) 459
PmlI CACGTG 1 cut(s) 459
PpsI GAGTC 3 cut(s) 439, 544, 1068
Ppu21I YACGTR 1 cut(s) 459
PshAI GACNNNNGTC 1 cut(s) 660
Psp124BI GAGCTC 3 cut(s) 500, 561, 623
Psp6I CCWGG 2 cut(s) 422, 471
PspCI CACGTG 1 cut(s) 459
PspEI GGTNACC 1 cut(s) 16
PspGI CCWGG 2 cut(s) 422, 471
PspLI CGTACG 1 cut(s) 902
PspN4I GGNNCC 8 cut(s) 112, 223, 235, 236, 271, 477, 778, 899
PspOMI GGGCCC 2 cut(s) 234, 776
PspPI GGNCC 6 cut(s) 154, 234, 235, 475, 776, 777
PspXI VCTCGAGB 1 cut(s) 41
PsuI RGATCY 2 cut(s) 27, 159
PsyI GACNNNGTC 2 cut(s) 212, 911
RsaI GTAC 3 cut(s) 287, 380, 904
RsaNI GTAC 3 cut(s) 286, 379, 903
SacI GAGCTC 3 cut(s) 500, 561, 623
SapI GCTCTTC 2 cut(s) 317, 488
SatI GCNGC 6 cut(s) 47, 246, 416, 574, 687, 690
Sau3AI GATC 4 cut(s) 27, 159, 178, 962
Sau96I GGNCC 6 cut(s) 154, 234, 235, 475, 776, 777
SchI GAGTC 3 cut(s) 439, 545, 1068
ScrFI CCNGG 2 cut(s) 424, 473
SduI GDGCHC 8 cut(s) 238, 500, 561, 623, 647, 780, 818, 846
SfaNI GCATC 2 cut(s) 237, 289
Sfr274I CTCGAG 2 cut(s) 41, 149
SinI GGWCC 2 cut(s) 154, 475
SlaI CTCGAG 2 cut(s) 41, 149
SmlI CTYRAG 5 cut(s) 41, 149, 167, 449, 781
SmoI CTYRAG 5 cut(s) 41, 149, 167, 449, 781
Sse9I AATT 2 cut(s) 331, 918
SseBI AGGCCT 4 cut(s) 71, 280, 926, 1135
SspMI CTAG 2 cut(s) 294, 345
SstI GAGCTC 3 cut(s) 500, 561, 623
StuI AGGCCT 4 cut(s) 71, 280, 926, 1135
StyD4I CCNGG 2 cut(s) 422, 471
StyI CCWWGG 2 cut(s) 21, 765
TaaI ACNGT 5 cut(s) 507, 865, 902, 1011, 1148
TaiI ACGT 5 cut(s) 461, 584, 641, 915, 1007
TaqI TCGA 8 cut(s) 42, 150, 207, 549, 555, 612, 846, 1056
TaqII GACCGA 1 cut(s) 301
TasI AATT 2 cut(s) 331, 918
TatI WGTACW 1 cut(s) 285
TauI GCSGC 4 cut(s) 248, 576, 689, 692
TfiI GAWTC 3 cut(s) 88, 1053, 1121
TscAI CASTG 3 cut(s) 68, 295, 946
TseFI GTSAC 4 cut(s) 16, 694, 719, 941
TseI GCWGC 2 cut(s) 46, 415
Tsp45I GTSAC 4 cut(s) 16, 694, 719, 941
TspDTI ATGAA 1 cut(s) 686
TspRI CASTG 3 cut(s) 68, 295, 946
Tth111I GACNNNGTC 2 cut(s) 212, 911
Van91I CCANNNNNTGG 1 cut(s) 877
VpaK11BI GGWCC 2 cut(s) 154, 475
XapI RAATTY 2 cut(s) 331, 918
XhoI CTCGAG 2 cut(s) 41, 149
XmnI GAANNNNTTC 2 cut(s) 335, 887
XspI CTAG 2 cut(s) 294, 345
ZraI GACGTC 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.