Rroxscaffold_7G00176480

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0015100
Physical Location & Seq
Reverse (-)
15933595 .. 15935001
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00176480.1

Sequence Viewer

Length: 951 bp
ATGGAGCTGAGTAAGGTGACCTTGGAGATCTTCACGAAGCTCGAGCAGCAATGGCTCTCTCACTGCGAGGCCACCAAGAAGACACGGATTCTCAGCATTGACGGTGGAGGAACCACCGGCATTGTCGCCGGACAAGCCCTTATCCATCTCGAGGACCAGATCCGCCTCAAGACTGGCGATCCCCACGCTCAAATCGCCGATTTCTTCGACCTCGTCGCCGGAACCGGCATCGGGGCCGTCCTCGCCGCCATGCTCGTCGCCGATGACGGCTCCGGCAGGCCTCTGTACACTGCTAGAGATGCTGTAAACTCGGTCGCCGAGAAGAGCGAGAATTTGTTCAAGGCTAGGGTGGTAGGAGTTTTCCGCCGTGGCCGGAGGTACTCCGGCGGAAGCATGGACAAGGCGCTGACGGAGCTGCTGACCAGGGAGGACGGCAAGGTCTTGACTCTCAAGGACACGTGTAAGCCTCTCCTGGTCCCCTGCTACGACTTGAAGAGCTCTGCACCGTTCGTTTTCTCCCGCGCCGACGCTTCCGAGTCGCCGAGTTTCGACTTCGAGCTCCGCAAAGTCTGCCGGGCCACCGGACGGAAGCCCCGCCGTAACGGCGAGTGCTCTCCCTCTTCCGTCGTTGACATTGTGCTTGACGGCGTTTCCGAGACCGTTGACCAGATGGTGGGGAACGCCTTCTGTTGGAACCGTACGGACTACGTCAGAATTCAGGCCTTTGGATTGGGTAGTGACGGAGATGTGGGGCGGAGATCAGAGCAAGTGGCGGTGTTGAAAGAGAGAGGAGTGGAGACGTTACCGTTTGGCGGGAAGCGGTTACTGACGGAGACAAACGGAGAGAGAATCGAGGGTTTCGTGCAACGACTTGTGGCTTGTGGAAGGAGCAGTTTGCCACCAAGTCCGTGCAAGGATTCGGCCGTTAGGCCTCTCGCTAACGGTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

316

Amino Acids

34.28

Weight (kDa)

8.59

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 31 - 195 3.7e-11 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63200
fragaria_vesca FvH4_2g23520 FvH4_2g23520
malus_domestica MD03G1264100.v1.1 MD11G1284700.v1.1
prunus_persica Prupe.8G240600_v2.0.a1
pyrus_communis pycom03g21180
rosa_chinensis RchiOBHm_Chr6g0290991
rosa_laevigata RLG00000012157
rosa_roxburghii Rroxscaffold_7G00176480
rosa_rugosa Rorug06G0217000
rosa_samantha Rh6AG326700 Rh6BG334200 Rh6CG340400 Rh6DG327600
rosa_wichuraiana Rw6G028410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 437
AccB7I CCANNNNNTGG 1 cut(s) 673
AccII CGCG 1 cut(s) 522
AclWI GGATC 2 cut(s) 154, 173
AcoI YGGCCR 2 cut(s) 370, 921
AcsI RAATTY 2 cut(s) 331, 714
AcvI CACGTG 1 cut(s) 459
AfaI GTAC 3 cut(s) 287, 380, 700
AfiI CCNNNNNNNGG 6 cut(s) 151, 231, 585, 673, 690, 812
AflIII ACRYGT 2 cut(s) 456, 458
AgsI TTSAA 3 cut(s) 340, 493, 781
AjnI CCWGG 2 cut(s) 422, 471
AluBI AGCT 5 cut(s) 7, 40, 415, 498, 559
AluI AGCT 5 cut(s) 7, 40, 415, 498, 559
Alw21I GWGCWC 3 cut(s) 500, 561, 614
Alw26I GTCTC 3 cut(s) 650, 791, 827
AlwI GGATC 2 cut(s) 154, 173
Ama87I CYCGRG 2 cut(s) 41, 149
AoxI GGCC 8 cut(s) 69, 234, 278, 370, 576, 720, 921, 929
ApeKI GCWGC 2 cut(s) 46, 415
ApoI RAATTY 2 cut(s) 331, 714
Asp700I GAANNNNTTC 2 cut(s) 335, 683
AspLEI GCGC 2 cut(s) 406, 524
AspS9I GGNCC 4 cut(s) 154, 234, 475, 576
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 2 cut(s) 41, 149
AvaII GGWCC 2 cut(s) 154, 475
BanII GRGCYC 2 cut(s) 500, 561
BbrPI CACGTG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 3 cut(s) 500, 561, 614
BbvI GCAGC 2 cut(s) 58, 402
BccI CCATC 2 cut(s) 153, 664
BceAI ACGGC 8 cut(s) 221, 283, 351, 448, 582, 619, 661, 908
BciT130I CCWGG 2 cut(s) 424, 473
BcnI CCSGG 1 cut(s) 575
BcoDI GTCTC 3 cut(s) 650, 791, 827
BfaI CTAG 2 cut(s) 294, 345
BfoI RGCGCY 1 cut(s) 407
BglI GCCNNNNNGGC 1 cut(s) 603
BglII AGATCT 1 cut(s) 27
BisI GCNGC 3 cut(s) 47, 246, 416
BlsI GCNGC 3 cut(s) 48, 247, 417
Bme1390I CCNGG 3 cut(s) 424, 473, 575
Bme18I GGWCC 2 cut(s) 154, 475
BmeT110I CYCGRG 2 cut(s) 41, 149
BmgT120I GGNCC 4 cut(s) 154, 234, 475, 576
BmiI GGNNCC 6 cut(s) 112, 223, 235, 271, 477, 695
BmrFI CCNGG 3 cut(s) 424, 473, 575
BmsI GCATC 2 cut(s) 237, 289
BpiI GAAGAC 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 152, 434
BpuMI CCSGG 1 cut(s) 575
BsaAI YACGTR 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 3 cut(s) 21, 367, 423
BsaWI WCCGGW 1 cut(s) 581
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 231, 585, 673, 690, 812
Bse118I RCCGGY 2 cut(s) 116, 224
Bse1I ACTGG 1 cut(s) 178
Bse3DI GCAATG 1 cut(s) 56
BseBI CCWGG 2 cut(s) 424, 473
BseDI CCNNGG 3 cut(s) 21, 367, 423
BseLI CCNNNNNNNGG 6 cut(s) 151, 231, 585, 673, 690, 812
BseMI GCAATG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 804
BseX3I CGGCCG 1 cut(s) 921
BseXI GCAGC 2 cut(s) 58, 402
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 1 cut(s) 522
Bsh1285I CGRYCG 3 cut(s) 315, 924, 946
BshFI GGCC 8 cut(s) 71, 236, 280, 372, 578, 722, 923, 931
BsiEI CGRYCG 3 cut(s) 315, 924, 946
BsiHKAI GWGCWC 3 cut(s) 500, 561, 614
BsiHKCI CYCGRG 2 cut(s) 41, 149
BsiSI CCGG 9 cut(s) 117, 129, 219, 225, 273, 373, 384, 574, 582
BsiWI CGTACG 1 cut(s) 698
BslFI GGGAC 1 cut(s) 461
BslI CCNNNNNNNGG 6 cut(s) 151, 231, 585, 673, 690, 812
BsmAI GTCTC 3 cut(s) 650, 791, 827
BsmBI CGTCTC 1 cut(s) 791
BsmFI GGGAC 1 cut(s) 461
BsnI GGCC 8 cut(s) 71, 236, 280, 372, 578, 722, 923, 931
Bso31I GGTCTC 1 cut(s) 650
BsoBI CYCGRG 2 cut(s) 41, 149
Bsp1286I GDGCHC 3 cut(s) 500, 561, 614
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 4 cut(s) 27, 159, 178, 758
BspANI GGCC 8 cut(s) 71, 236, 280, 372, 578, 722, 923, 931
BspCNI CTCAG 1 cut(s) 105
BspFNI CGCG 1 cut(s) 522
BspLI GGNNCC 6 cut(s) 112, 223, 235, 271, 477, 695
BspPI GGATC 2 cut(s) 154, 173
BspQI GCTCTTC 2 cut(s) 317, 488
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 56
BsrFI RCCGGY 2 cut(s) 116, 224
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 178
BssAI RCCGGY 2 cut(s) 116, 224
BssECI CCNNGG 3 cut(s) 21, 367, 423
BssMI GATC 4 cut(s) 27, 159, 178, 758
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 2 cut(s) 424, 473
Bst4CI ACNGT 6 cut(s) 104, 507, 661, 698, 807, 944
Bst6I CTCTTC 3 cut(s) 317, 488, 625
BstAPI GCANNNNNTGC 1 cut(s) 570
BstAUI TGTACA 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 459
BstC8I GCNNGC 1 cut(s) 278
BstDEI CTNAG 2 cut(s) 8, 92
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 16
BstFNI CGCG 1 cut(s) 522
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 2 cut(s) 406, 524
BstKTI GATC 4 cut(s) 30, 162, 181, 761
BstMAI GTCTC 3 cut(s) 650, 791, 827
BstMBI GATC 4 cut(s) 27, 159, 178, 758
BstMCI CGRYCG 3 cut(s) 315, 924, 946
BstMWI GCNNNNNNNGC 9 cut(s) 46, 52, 134, 194, 242, 299, 412, 570, 603
BstNI CCWGG 2 cut(s) 424, 473
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 3 cut(s) 422, 471, 573
BstUI CGCG 1 cut(s) 522
BstV1I GCAGC 2 cut(s) 58, 402
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 2 cut(s) 27, 159
BstYI RGATCY 2 cut(s) 27, 159
BstZI CGGCCG 1 cut(s) 921
BsuRI GGCC 8 cut(s) 71, 236, 280, 372, 578, 722, 923, 931
BtgI CCRYGG 1 cut(s) 367
BtsI GCAGTG 2 cut(s) 61, 288
BtsIMutI CAGTG 2 cut(s) 61, 288
Cac8I GCNNGC 1 cut(s) 278
CfoI GCGC 2 cut(s) 406, 524
Cfr10I RCCGGY 2 cut(s) 116, 224
Cfr13I GGNCC 4 cut(s) 154, 234, 475, 576
CseI GACGC 1 cut(s) 536
Csp6I GTAC 3 cut(s) 286, 379, 699
CspCI CAANNNNNGTGG 2 cut(s) 103, 138
CviAII CATG 2 cut(s) 250, 394
CviQI GTAC 3 cut(s) 286, 379, 699
DdeI CTNAG 2 cut(s) 8, 92
DpnI GATC 4 cut(s) 29, 161, 180, 760
DpnII GATC 4 cut(s) 27, 159, 178, 758
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 2 cut(s) 370, 921
EagI CGGCCG 1 cut(s) 921
Eam1104I CTCTTC 3 cut(s) 317, 488, 625
EarI CTCTTC 3 cut(s) 317, 488, 625
EciI GGCGGA 4 cut(s) 152, 353, 402, 769
Ecl136II GAGCTC 2 cut(s) 498, 559
EclXI CGGCCG 1 cut(s) 921
Eco130I CCWWGG 1 cut(s) 21
Eco147I AGGCCT 3 cut(s) 280, 722, 931
Eco24I GRGCYC 2 cut(s) 500, 561
Eco31I GGTCTC 1 cut(s) 650
Eco47I GGWCC 2 cut(s) 154, 475
Eco52I CGGCCG 1 cut(s) 921
Eco53kI GAGCTC 2 cut(s) 498, 559
Eco72I CACGTG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 41, 149
Eco91I GGTNACC 1 cut(s) 16
EcoICRI GAGCTC 2 cut(s) 498, 559
EcoO65I GGTNACC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 2 cut(s) 422, 471
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 2 cut(s) 500, 561
ErhI CCWWGG 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 791
FaeI CATG 2 cut(s) 253, 397
FaiI YATR 2 cut(s) 251, 395
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 1 cut(s) 461
FatI CATG 2 cut(s) 249, 393
FauI CCCGC 3 cut(s) 527, 602, 806
Fnu4HI GCNGC 3 cut(s) 47, 246, 416
FriOI GRGCYC 2 cut(s) 500, 561
Fsp4HI GCNGC 3 cut(s) 47, 246, 416
FspBI CTAG 2 cut(s) 294, 345
GlaI GCGC 2 cut(s) 405, 523
GluI GCNGC 3 cut(s) 47, 246, 416
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 8 cut(s) 71, 236, 280, 372, 578, 722, 923, 931
HapII CCGG 9 cut(s) 117, 129, 219, 225, 273, 373, 384, 574, 582
HgaI GACGC 1 cut(s) 536
HhaI GCGC 2 cut(s) 406, 524
Hin1II CATG 2 cut(s) 253, 397
Hin6I GCGC 2 cut(s) 404, 522
HinP1I GCGC 2 cut(s) 404, 522
HincII GTYRAC 2 cut(s) 631, 664
HindII GTYRAC 2 cut(s) 631, 664
HinfI GANTC 5 cut(s) 88, 445, 536, 849, 917
HpaII CCGG 9 cut(s) 117, 129, 219, 225, 273, 373, 384, 574, 582
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 4 cut(s) 288, 307, 631, 664
Hpy188I TCNGA 4 cut(s) 535, 655, 713, 763
Hpy188III TCNNGA 4 cut(s) 34, 149, 169, 442
Hpy8I GTNNAC 4 cut(s) 288, 307, 631, 664
Hpy99I CGWCG 4 cut(s) 218, 260, 530, 629
HpyAV CCTTC 2 cut(s) 694, 879
HpyCH4III ACNGT 6 cut(s) 104, 507, 661, 698, 807, 944
HpyCH4IV ACGT 3 cut(s) 458, 708, 800
HpyCH4V TGCA 3 cut(s) 503, 865, 912
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 52, 134, 194, 242, 299, 412, 570, 603
HpyF3I CTNAG 2 cut(s) 8, 92
HpySE526I ACGT 3 cut(s) 458, 708, 800
Hsp92II CATG 2 cut(s) 253, 397
HspAI GCGC 2 cut(s) 404, 522
Kzo9I GATC 4 cut(s) 27, 159, 178, 758
LguI GCTCTTC 2 cut(s) 317, 488
LmnI GCTCC 5 cut(s) 4, 275, 412, 564, 888
Lsp1109I GCAGC 2 cut(s) 58, 402
LweI GCATC 2 cut(s) 237, 289
MaeI CTAG 2 cut(s) 294, 345
MaeII ACGT 3 cut(s) 458, 708, 800
MaeIII GTNAC 5 cut(s) 16, 599, 737, 801, 822
MalI GATC 4 cut(s) 29, 161, 180, 760
MboI GATC 4 cut(s) 27, 159, 178, 758
MboII GAAGA 6 cut(s) 22, 91, 196, 334, 505, 612
MflI RGATCY 2 cut(s) 27, 159
MhlI GDGCHC 3 cut(s) 500, 561, 614
MluCI AATT 2 cut(s) 331, 714
MlyI GAGTC 2 cut(s) 439, 545
MmeI TCCRAC 1 cut(s) 671
MroXI GAANNNNTTC 2 cut(s) 335, 683
MspI CCGG 9 cut(s) 117, 129, 219, 225, 273, 373, 384, 574, 582
MspR9I CCNGG 3 cut(s) 424, 473, 575
MvaI CCWGG 2 cut(s) 424, 473
MvnI CGCG 1 cut(s) 522
MwoI GCNNNNNNNGC 9 cut(s) 46, 52, 134, 194, 242, 299, 412, 570, 603
NciI CCSGG 1 cut(s) 575
NdeII GATC 4 cut(s) 27, 159, 178, 758
NlaIII CATG 2 cut(s) 253, 397
NlaIV GGNNCC 6 cut(s) 112, 223, 235, 271, 477, 695
NmeAIII GCCGAG 2 cut(s) 343, 567
NmuCI GTSAC 2 cut(s) 16, 737
PaeR7I CTCGAG 2 cut(s) 41, 149
PceI AGGCCT 3 cut(s) 280, 722, 931
PciSI GCTCTTC 2 cut(s) 317, 488
PcsI WCGNNNNNNNCGW 2 cut(s) 651, 858
PdmI GAANNNNTTC 2 cut(s) 335, 683
PfeI GAWTC 3 cut(s) 88, 849, 917
Pfl23II CGTACG 1 cut(s) 698
PflFI GACNNNGTC 2 cut(s) 212, 707
PflMI CCANNNNNTGG 1 cut(s) 673
PkrI GCNGC 3 cut(s) 48, 247, 417
PleI GAGTC 2 cut(s) 439, 544
PmaCI CACGTG 1 cut(s) 459
PmlI CACGTG 1 cut(s) 459
PpsI GAGTC 2 cut(s) 439, 544
Ppu21I YACGTR 1 cut(s) 459
Psp124BI GAGCTC 2 cut(s) 500, 561
Psp6I CCWGG 2 cut(s) 422, 471
PspCI CACGTG 1 cut(s) 459
PspEI GGTNACC 1 cut(s) 16
PspGI CCWGG 2 cut(s) 422, 471
PspLI CGTACG 1 cut(s) 698
PspN4I GGNNCC 6 cut(s) 112, 223, 235, 271, 477, 695
PspPI GGNCC 4 cut(s) 154, 234, 475, 576
PspXI VCTCGAGB 1 cut(s) 41
PsuI RGATCY 2 cut(s) 27, 159
PsyI GACNNNGTC 2 cut(s) 212, 707
RsaI GTAC 3 cut(s) 287, 380, 700
RsaNI GTAC 3 cut(s) 286, 379, 699
SacI GAGCTC 2 cut(s) 500, 561
SapI GCTCTTC 2 cut(s) 317, 488
SatI GCNGC 3 cut(s) 47, 246, 416
Sau3AI GATC 4 cut(s) 27, 159, 178, 758
Sau96I GGNCC 4 cut(s) 154, 234, 475, 576
SchI GAGTC 2 cut(s) 439, 545
ScrFI CCNGG 3 cut(s) 424, 473, 575
SduI GDGCHC 3 cut(s) 500, 561, 614
SfaNI GCATC 2 cut(s) 237, 289
Sfr274I CTCGAG 2 cut(s) 41, 149
SinI GGWCC 2 cut(s) 154, 475
SlaI CTCGAG 2 cut(s) 41, 149
SmlI CTYRAG 4 cut(s) 41, 149, 167, 449
SmoI CTYRAG 4 cut(s) 41, 149, 167, 449
Sse9I AATT 2 cut(s) 331, 714
SseBI AGGCCT 3 cut(s) 280, 722, 931
SspMI CTAG 2 cut(s) 294, 345
SstI GAGCTC 2 cut(s) 500, 561
StuI AGGCCT 3 cut(s) 280, 722, 931
StyD4I CCNGG 3 cut(s) 422, 471, 573
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 6 cut(s) 104, 507, 661, 698, 807, 944
TaiI ACGT 3 cut(s) 461, 711, 803
TaqI TCGA 6 cut(s) 42, 150, 207, 549, 555, 852
TaqII GACCGA 1 cut(s) 301
TasI AATT 2 cut(s) 331, 714
TatI WGTACW 1 cut(s) 285
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 3 cut(s) 88, 849, 917
TscAI CASTG 2 cut(s) 68, 295
TseFI GTSAC 2 cut(s) 16, 737
TseI GCWGC 2 cut(s) 46, 415
Tsp45I GTSAC 2 cut(s) 16, 737
TspGWI ACGGA 9 cut(s) 100, 425, 601, 613, 716, 756, 845, 855, 897
TspRI CASTG 2 cut(s) 68, 295
Tth111I GACNNNGTC 2 cut(s) 212, 707
Van91I CCANNNNNTGG 1 cut(s) 673
VpaK11BI GGWCC 2 cut(s) 154, 475
XapI RAATTY 2 cut(s) 331, 714
XhoI CTCGAG 2 cut(s) 41, 149
XmnI GAANNNNTTC 2 cut(s) 335, 683
XspI CTAG 2 cut(s) 294, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.