MD11G1284700.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015100
Physical Location & Seq
Forward (+)
40311288 .. 40312941
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1284700.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGGAGCTGAGCGAGGTGACCTTAGAGATCTTCACGAAGCTGGAGCAGAAGTGGCTCTCCCACCGCGACACCACCAAGAAGACTCGGATTCTTAGCATCGACGGCGGAGGCACAACCGGCATTGTCGCCGGAGCCGCCTTGATCCACCTCGAGGATCAGATCCGACTAAAAACCGGAGATTCCCATGCTCAAATCGCCGATTACTTCGACCTCGTCACCGGTACCGGCATCGGTGCCGTCCTCGCCGCCATGCTCGTCGCCGATGACGGCTCCGGCCGCCCTCTCTTCACCGCTAAAGATGCTGTGAACTCGGTTTTCGAGAAGAATGCTGACTTGTTCAAATTGAGGTTCGGTGGAGTGCTCCGCCGGCACCGGAGGTTCTCCGGCAGGAGCATGGAAAAGGCGTTGGCGGAGCTTCTGACGAGGGAGGACGGCAAGGTTCTGACGCTCAAGGACACGTGTAAGCCTCTCCTGGTTCCCTGCTACGACATGAAGAGCTCCGCCCCGTTCGTGTTCTCCAGAGCCGACGCGTCCGAGTCGGCGAGTTTCGACTTCGAGCTGTGGAAAGTCTGCCGCGCCACGTCATCGACTCCGGGCTTGTTCAAGCCGCTCAGCCTGAGATCCGTGGACGGAAAGACTTCGTGCTCGGCCGTGGACGGCGGTCTTGTGATGAACAACCCGACGGCGGCAGCTGTTACGCACGTGCTCAACAACAAGCGCGACTTCCCCTCCGTCAATGGCGTCGAGGACCTGCTGGTTTTGTCGTTAGGTAACGGCCCGCTGAGCGTGGAGAAGGCGGGGCGGGGTAACGGAAAGTGCTCGCCCTCGTCGGTGGTTGACATTGTGCTCGACGGAGTTTCCGAGACCATTGACCAGATGATGGGAAACGCCTTCTGTTATAACCGCGCTGATTACGTCAGAATTCAGGCGTTCGGATTGGGGAGCGAAGGGGTTGCGGGCCAGCGATCGGAGGGGGAAGCGGCGGTGTTGAAGGAGAGAGGAGTGGAATCGTTACCGTTTGGCGGGAAGCGGTTATTGACGGAGACAAACGGAGAGAGAATCGATGGTTTCGTGCAACGACTCGTGGCTTGTGGAAGGAGCAGTCTGCCACCTAGTCCCTGCAAGGATTCCACCGTTAGCCCTGTCGTTAACGGGCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

387

Amino Acids

41.29

Weight (kDa)

7.56

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 31 - 231 1.3e-22 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63200
fragaria_vesca FvH4_2g23520 FvH4_2g23520
malus_domestica MD03G1264100.v1.1 MD11G1284700.v1.1
prunus_persica Prupe.8G240600_v2.0.a1
pyrus_communis pycom03g21180
rosa_chinensis RchiOBHm_Chr6g0290991
rosa_laevigata RLG00000012157
rosa_roxburghii Rroxscaffold_7G00176480
rosa_rugosa Rorug06G0217000
rosa_samantha Rh6AG326700 Rh6BG334200 Rh6CG340400 Rh6DG327600
rosa_wichuraiana Rw6G028410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 900
AbsI CCTCGAGG 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 759
Acc65I GGTACC 1 cut(s) 221
AccB1I GGYRCC 3 cut(s) 221, 233, 369
AccB7I CCANNNNNTGG 1 cut(s) 880
AccBSI CCGCTC 1 cut(s) 610
AccII CGCG 5 cut(s) 66, 530, 576, 720, 906
AclWI GGATC 4 cut(s) 136, 154, 162, 615
AcoI YGGCCR 2 cut(s) 274, 648
AcsI RAATTY 1 cut(s) 921
AcvI CACGTG 2 cut(s) 459, 703
AcyI GRCGYC 1 cut(s) 741
AfaI GTAC 1 cut(s) 223
AfiI CCNNNNNNNGG 5 cut(s) 151, 685, 880, 967, 1022
AflIII ACRYGT 3 cut(s) 456, 458, 528
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 3 cut(s) 340, 604, 991
AjiI CACGTC 1 cut(s) 582
AjnI CCWGG 1 cut(s) 471
AluBI AGCT 6 cut(s) 7, 40, 415, 498, 559, 692
AluI AGCT 6 cut(s) 7, 40, 415, 498, 559, 692
Alw21I GWGCWC 6 cut(s) 363, 500, 647, 708, 821, 849
Alw26I GTCTC 2 cut(s) 857, 1037
AlwI GGATC 4 cut(s) 136, 154, 162, 615
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 4 cut(s) 274, 648, 775, 958
ApeKI GCWGC 1 cut(s) 689
ApoI RAATTY 1 cut(s) 921
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 637
Asp718I GGTACC 1 cut(s) 221
AspLEI GCGC 3 cut(s) 578, 720, 908
AspS9I GGNCC 3 cut(s) 748, 776, 958
AsuC2I CCSGG 1 cut(s) 594
AsuHPI GGTGA 3 cut(s) 28, 208, 280
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 1 cut(s) 748
BanI GGYRCC 3 cut(s) 221, 233, 369
BanII GRGCYC 1 cut(s) 500
BauI CACGAG 1 cut(s) 1082
BbrPI CACGTG 2 cut(s) 459, 703
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 6 cut(s) 363, 500, 647, 708, 821, 849
BbvI GCAGC 1 cut(s) 701
BccI CCATC 2 cut(s) 874, 1058
BceAI ACGGC 8 cut(s) 118, 221, 283, 448, 635, 673, 699, 790
BcgI CGANNNNNNTGC 4 cut(s) 308, 342, 935, 969
BciT130I CCWGG 1 cut(s) 473
BcnI CCSGG 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 857, 1037
BfaI CTAG 1 cut(s) 1113
BfuAI ACCTGC 1 cut(s) 759
BglII AGATCT 1 cut(s) 27
BisI GCNGC 8 cut(s) 135, 246, 277, 574, 608, 687, 690, 981
BlpI GCTNAGC 3 cut(s) 8, 611, 782
BlsI GCNGC 8 cut(s) 136, 247, 278, 575, 609, 688, 691, 982
Bme1390I CCNGG 2 cut(s) 473, 594
Bme18I GGWCC 1 cut(s) 748
BmeT110I CYCGRG 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 582
BmgT120I GGNCC 3 cut(s) 748, 776, 958
BmiI GGNNCC 6 cut(s) 133, 223, 235, 271, 371, 477
BmrFI CCNGG 2 cut(s) 473, 594
BmsI GCATC 3 cut(s) 105, 237, 289
BoxI GACNNNNGTC 1 cut(s) 660
BpiI GAAGAC 1 cut(s) 86
BpmI CTGGAG 2 cut(s) 62, 502
Bpu1102I GCTNAGC 3 cut(s) 8, 611, 782
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 594
Bsa29I ATCGAT 1 cut(s) 1062
BsaAI YACGTR 2 cut(s) 459, 703
BsaHI GRCGYC 1 cut(s) 741
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 2 cut(s) 624, 651
BsaWI WCCGGW 3 cut(s) 173, 218, 372
BsaXI ACNNNNNCTCC 4 cut(s) 35, 65, 713, 743
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 685, 880, 967, 1022
Bse118I RCCGGY 4 cut(s) 116, 218, 224, 366
BseBI CCWGG 1 cut(s) 473
BseCI ATCGAT 1 cut(s) 1062
BseDI CCNNGG 2 cut(s) 624, 651
BseLI CCNNNNNNNGG 5 cut(s) 151, 685, 880, 967, 1022
BseMII CTCAG 3 cut(s) 608, 625, 773
BseRI GAGGAG 1 cut(s) 1014
BseX3I CGGCCG 2 cut(s) 274, 648
BseXI GCAGC 1 cut(s) 701
Bsh1236I CGCG 5 cut(s) 66, 530, 576, 720, 906
Bsh1285I CGRYCG 3 cut(s) 277, 651, 968
BshFI GGCC 4 cut(s) 276, 650, 777, 960
BshNI GGYRCC 3 cut(s) 221, 233, 369
BshTI ACCGGT 1 cut(s) 218
BshVI ATCGAT 1 cut(s) 1062
BsiEI CGRYCG 3 cut(s) 277, 651, 968
BsiHKAI GWGCWC 6 cut(s) 363, 500, 647, 708, 821, 849
BsiHKCI CYCGRG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 1101
BslI CCNNNNNNNGG 5 cut(s) 151, 685, 880, 967, 1022
BsmAI GTCTC 2 cut(s) 857, 1037
BsmFI GGGAC 1 cut(s) 1101
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 4 cut(s) 276, 650, 777, 960
Bso31I GGTCTC 1 cut(s) 857
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 6 cut(s) 363, 500, 647, 708, 821, 849
Bsp143I GATC 6 cut(s) 27, 141, 154, 159, 620, 965
Bsp1720I GCTNAGC 3 cut(s) 8, 611, 782
BspANI GGCC 4 cut(s) 276, 650, 777, 960
BspCNI CTCAG 3 cut(s) 609, 624, 774
BspDI ATCGAT 1 cut(s) 1062
BspFNI CGCG 5 cut(s) 66, 530, 576, 720, 906
BspLI GGNNCC 6 cut(s) 133, 223, 235, 271, 371, 477
BspMI ACCTGC 1 cut(s) 759
BspPI GGATC 4 cut(s) 136, 154, 162, 615
BspQI GCTCTTC 1 cut(s) 488
BspT107I GGYRCC 3 cut(s) 221, 233, 369
BspTNI GGTCTC 1 cut(s) 857
BsrBI CCGCTC 1 cut(s) 610
BsrFI RCCGGY 4 cut(s) 116, 218, 224, 366
BssAI RCCGGY 4 cut(s) 116, 218, 224, 366
BssECI CCNNGG 2 cut(s) 624, 651
BssMI GATC 6 cut(s) 27, 141, 154, 159, 620, 965
BssNI GRCGYC 1 cut(s) 741
BssSI CACGAG 1 cut(s) 1082
Bst2BI CACGAG 1 cut(s) 1082
Bst2UI CCWGG 1 cut(s) 473
Bst4CI ACNGT 2 cut(s) 1017, 1135
Bst6I CTCTTC 2 cut(s) 290, 488
BstACI GRCGYC 1 cut(s) 741
BstBAI YACGTR 2 cut(s) 459, 703
BstC8I GCNNGC 5 cut(s) 368, 779, 821, 958, 962
BstDEI CTNAG 6 cut(s) 8, 22, 92, 611, 617, 782
BstDSI CCRYGG 2 cut(s) 624, 651
BstEII GGTNACC 1 cut(s) 16
BstFNI CGCG 5 cut(s) 66, 530, 576, 720, 906
BstHHI GCGC 3 cut(s) 578, 720, 908
BstKTI GATC 6 cut(s) 30, 144, 157, 162, 623, 968
BstMAI GTCTC 2 cut(s) 857, 1037
BstMBI GATC 6 cut(s) 27, 141, 154, 159, 620, 965
BstMCI CGRYCG 3 cut(s) 277, 651, 968
BstNI CCWGG 1 cut(s) 473
BstPAI GACNNNNGTC 1 cut(s) 660
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 2 cut(s) 471, 592
BstUI CGCG 5 cut(s) 66, 530, 576, 720, 906
BstV1I GCAGC 1 cut(s) 701
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 3 cut(s) 27, 159, 620
BstYI RGATCY 3 cut(s) 27, 159, 620
BstZI CGGCCG 2 cut(s) 274, 648
Bsu15I ATCGAT 1 cut(s) 1062
BsuRI GGCC 4 cut(s) 276, 650, 777, 960
BsuTUI ATCGAT 1 cut(s) 1062
BtgI CCRYGG 2 cut(s) 624, 651
BtrI CACGTC 1 cut(s) 582
BveI ACCTGC 1 cut(s) 759
Cac8I GCNNGC 5 cut(s) 368, 779, 821, 958, 962
CfoI GCGC 3 cut(s) 578, 720, 908
Cfr10I RCCGGY 4 cut(s) 116, 218, 224, 366
Cfr13I GGNCC 3 cut(s) 748, 776, 958
ClaI ATCGAT 1 cut(s) 1062
CseI GACGC 4 cut(s) 454, 519, 536, 730
Csp6I GTAC 1 cut(s) 222
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 4 cut(s) 185, 250, 394, 490
CviQI GTAC 1 cut(s) 222
DdeI CTNAG 6 cut(s) 8, 22, 92, 611, 617, 782
DpnI GATC 6 cut(s) 29, 143, 156, 161, 622, 967
DpnII GATC 6 cut(s) 27, 141, 154, 159, 620, 965
EaeI YGGCCR 2 cut(s) 274, 648
EagI CGGCCG 2 cut(s) 274, 648
Eam1104I CTCTTC 2 cut(s) 290, 488
EarI CTCTTC 2 cut(s) 290, 488
EciI GGCGGA 4 cut(s) 120, 353, 425, 490
Ecl136II GAGCTC 1 cut(s) 498
EclXI CGGCCG 2 cut(s) 274, 648
Eco24I GRGCYC 1 cut(s) 500
Eco31I GGTCTC 1 cut(s) 857
Eco47I GGWCC 1 cut(s) 748
Eco52I CGGCCG 2 cut(s) 274, 648
Eco53kI GAGCTC 1 cut(s) 498
Eco72I CACGTG 2 cut(s) 459, 703
Eco88I CYCGRG 1 cut(s) 149
Eco91I GGTNACC 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 498
EcoO109I RGGNCCY 1 cut(s) 748
EcoO65I GGTNACC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 921
EcoRII CCWGG 1 cut(s) 471
EcoT38I GRGCYC 1 cut(s) 500
FaeI CATG 4 cut(s) 188, 253, 397, 493
FaiI YATR 5 cut(s) 186, 251, 395, 491, 900
FalI AAGNNNNNCTT 2 cut(s) 707, 739
FaqI GGGAC 1 cut(s) 1101
FatI CATG 4 cut(s) 184, 249, 393, 489
FauI CCCGC 5 cut(s) 786, 790, 795, 949, 1016
Fnu4HI GCNGC 8 cut(s) 135, 246, 277, 574, 608, 687, 690, 981
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 8 cut(s) 135, 246, 277, 574, 608, 687, 690, 981
FspBI CTAG 1 cut(s) 1113
GlaI GCGC 3 cut(s) 577, 719, 907
GluI GCNGC 8 cut(s) 135, 246, 277, 574, 608, 687, 690, 981
GsuI CTGGAG 2 cut(s) 62, 502
HaeIII GGCC 4 cut(s) 276, 650, 777, 960
HgaI GACGC 4 cut(s) 454, 519, 536, 730
HhaI GCGC 3 cut(s) 578, 720, 908
Hin1I GRCGYC 1 cut(s) 741
Hin1II CATG 4 cut(s) 188, 253, 397, 493
Hin6I GCGC 3 cut(s) 576, 718, 906
HinP1I GCGC 3 cut(s) 576, 718, 906
HincII GTYRAC 2 cut(s) 838, 1150
HindII GTYRAC 2 cut(s) 838, 1150
HinfI GANTC 9 cut(s) 82, 88, 179, 536, 589, 1007, 1059, 1080, 1127
HpaI GTTAAC 1 cut(s) 1150
HphI GGTGA 3 cut(s) 28, 208, 280
Hpy166II GTNNAC 5 cut(s) 307, 628, 655, 838, 1150
Hpy188III TCNNGA 3 cut(s) 34, 319, 519
Hpy8I GTNNAC 5 cut(s) 307, 628, 655, 838, 1150
Hpy99I CGWCG 7 cut(s) 104, 260, 530, 685, 746, 832, 854
HpyAV CCTTC 5 cut(s) 787, 901, 941, 985, 1089
HpyCH4III ACNGT 2 cut(s) 1017, 1135
HpyCH4IV ACGT 4 cut(s) 458, 581, 702, 915
HpyCH4V TGCA 2 cut(s) 1075, 1122
HpyF3I CTNAG 6 cut(s) 8, 22, 92, 611, 617, 782
HpySE526I ACGT 4 cut(s) 458, 581, 702, 915
Hsp92I GRCGYC 1 cut(s) 741
Hsp92II CATG 4 cut(s) 188, 253, 397, 493
HspAI GCGC 3 cut(s) 576, 718, 906
KpnI GGTACC 1 cut(s) 225
KroI GCCGGC 1 cut(s) 366
KroNI GCCGGC 1 cut(s) 368
KspAI GTTAAC 1 cut(s) 1150
Kzo9I GATC 6 cut(s) 27, 141, 154, 159, 620, 965
LguI GCTCTTC 1 cut(s) 488
Lsp1109I GCAGC 1 cut(s) 701
LweI GCATC 3 cut(s) 105, 237, 289
MaeI CTAG 1 cut(s) 1113
MaeII ACGT 4 cut(s) 458, 581, 702, 915
MaeIII GTNAC 6 cut(s) 16, 214, 694, 770, 806, 1011
MalI GATC 6 cut(s) 29, 143, 156, 161, 622, 967
MbiI CCGCTC 1 cut(s) 610
MboI GATC 6 cut(s) 27, 141, 154, 159, 620, 965
MboII GAAGA 5 cut(s) 22, 91, 277, 334, 505
MflI RGATCY 3 cut(s) 27, 159, 620
MhlI GDGCHC 6 cut(s) 363, 500, 647, 708, 821, 849
MluCI AATT 2 cut(s) 341, 921
MluI ACGCGT 1 cut(s) 528
MlyI GAGTC 4 cut(s) 76, 545, 583, 1074
MmeI TCCRAC 1 cut(s) 187
MroNI GCCGGC 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 637
MseI TTAA 1 cut(s) 1149
MspA1I CMGCKG 2 cut(s) 692, 781
MspR9I CCNGG 2 cut(s) 473, 594
Mva1269I GAATGC 1 cut(s) 331
MvaI CCWGG 1 cut(s) 473
MvnI CGCG 5 cut(s) 66, 530, 576, 720, 906
NaeI GCCGGC 1 cut(s) 368
NciI CCSGG 1 cut(s) 594
NdeII GATC 6 cut(s) 27, 141, 154, 159, 620, 965
NgoMIV GCCGGC 1 cut(s) 366
NlaIII CATG 4 cut(s) 188, 253, 397, 493
NlaIV GGNNCC 6 cut(s) 133, 223, 235, 271, 371, 477
NmeAIII GCCGAG 1 cut(s) 626
NmuCI GTSAC 2 cut(s) 16, 214
PaeR7I CTCGAG 1 cut(s) 149
PciSI GCTCTTC 1 cut(s) 488
PcsI WCGNNNNNNNCGW 2 cut(s) 858, 1068
PctI GAATGC 1 cut(s) 331
PdiI GCCGGC 1 cut(s) 368
PdmI GAANNNNTTC 1 cut(s) 637
PfeI GAWTC 5 cut(s) 88, 179, 1007, 1059, 1127
PflFI GACNNNGTC 1 cut(s) 212
PflMI CCANNNNNTGG 1 cut(s) 880
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 8 cut(s) 136, 247, 278, 575, 609, 688, 691, 982
Ple19I CGATCG 1 cut(s) 968
PleI GAGTC 4 cut(s) 76, 544, 583, 1074
PmaCI CACGTG 2 cut(s) 459, 703
PmlI CACGTG 2 cut(s) 459, 703
PpsI GAGTC 4 cut(s) 76, 544, 583, 1074
Ppu21I YACGTR 2 cut(s) 459, 703
PpuMI RGGWCCY 1 cut(s) 748
PshAI GACNNNNGTC 1 cut(s) 660
PsiI TTATAA 1 cut(s) 900
Psp124BI GAGCTC 1 cut(s) 500
Psp5II RGGWCCY 1 cut(s) 748
Psp6I CCWGG 1 cut(s) 471
PspCI CACGTG 2 cut(s) 459, 703
PspEI GGTNACC 1 cut(s) 16
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 6 cut(s) 133, 223, 235, 271, 371, 477
PspPI GGNCC 3 cut(s) 748, 776, 958
PspPPI RGGWCCY 1 cut(s) 748
PspXI VCTCGAGB 1 cut(s) 149
PsuI RGATCY 3 cut(s) 27, 159, 620
PsyI GACNNNGTC 1 cut(s) 212
PvuI CGATCG 1 cut(s) 968
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
SacI GAGCTC 1 cut(s) 500
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 1 cut(s) 1149
SatI GCNGC 8 cut(s) 135, 246, 277, 574, 608, 687, 690, 981
Sau3AI GATC 6 cut(s) 27, 141, 154, 159, 620, 965
Sau96I GGNCC 3 cut(s) 748, 776, 958
SchI GAGTC 4 cut(s) 76, 545, 583, 1074
ScrFI CCNGG 2 cut(s) 473, 594
SduI GDGCHC 6 cut(s) 363, 500, 647, 708, 821, 849
SfaNI GCATC 3 cut(s) 105, 237, 289
Sfr274I CTCGAG 1 cut(s) 149
SinI GGWCC 1 cut(s) 748
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 2 cut(s) 149, 449
SmoI CTYRAG 2 cut(s) 149, 449
Sse9I AATT 2 cut(s) 341, 921
SspMI CTAG 1 cut(s) 1113
SstI GAGCTC 1 cut(s) 500
StyD4I CCNGG 2 cut(s) 471, 592
TaaI ACNGT 2 cut(s) 1017, 1135
TaiI ACGT 4 cut(s) 461, 584, 705, 918
TasI AATT 2 cut(s) 341, 921
TauI GCSGC 7 cut(s) 137, 248, 279, 576, 610, 689, 983
TfiI GAWTC 5 cut(s) 88, 179, 1007, 1059, 1127
Tru1I TTAA 1 cut(s) 1149
Tru9I TTAA 1 cut(s) 1149
TseFI GTSAC 2 cut(s) 16, 214
TseI GCWGC 1 cut(s) 689
Tsp45I GTSAC 2 cut(s) 16, 214
TspDTI ATGAA 2 cut(s) 506, 686
TspGWI ACGGA 7 cut(s) 613, 645, 721, 825, 867, 1055, 1065
Tth111I GACNNNGTC 1 cut(s) 212
Van91I CCANNNNNTGG 1 cut(s) 880
VpaK11BI GGWCC 1 cut(s) 748
XapI RAATTY 1 cut(s) 921
XhoI CTCGAG 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 637
XspI CTAG 1 cut(s) 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.