AT4G00200

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0018289
Physical Location & Seq
Reverse (-)
82309 .. 84770
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00200.2

Sequence Viewer

Length: 957 bp
ATGGAAACAAGCGACAGAATTAGCCCTGGTGGTGGTATAGGAGCAGAAGTTCCATCAGCGTATCATATGGCTCCGAGACCTTCAGATAGCCCTGCTAACCAGTTTATGGGGTTGTCTCTCCCTCCCATGGAAGCCCCAATGCCTTCTTCAGGGGAAGCCTCAGGGAAGAAGAGGAGAGGCAGACCTCGTAAATATGAAGCTAATGGTGCTCCTTTGCCTTCTTCATCAGTTCCTCTTGTGAAGAAACGAGTAAGAGGCAAGCTCAATGGTTTTGATATGAAGAAGATGCATAAGACCATTGGGTTCCATTCTTCTGGTGAAAGGTTTGGTGTTGGTGGTGGTGTTGGTGGTGGTGTTGGATCAAATTTCACGCCGCATGTAATCACAGTGAACACTGGTGAGGATATTACTATGAGAATCATCTCCTTTTCACAACAAGGACCACGAGCTATTTGTATCTTATCAGCAAATGGAGTGATATCAAACGTTACTCTTCGCCAACCCGATTCTTGCGGTGGTACTCTCACCTACGAGGGTCGGTTTGAGATACTGTCTCTGTCTGGTTCATTCATGGAAACAGAGAATCAAGGTTCAAAAGGTCGGTCTGGTGGCATGAGTGTATCTTTGGCCGGTCCTGATGGTCGTGTTGTCGGTGGCGGTGTCGCTGGCCTGCTCATTGCCGCCACTCCTATTCAGGTTGTTGTTGGCAGTTTTATAACGAGTGATCAACAAGATCATCAGAAACCGAGGAAGCAAAGAGTCGAGCATGCCCCTGCAGCAGTCATGTCAGTGCCTCCTCCTCCTTCTCCTCCTCCTCCGGCGGCTTCTGTTTTCTCACCGACAAACCCGGACAGAGAGCAGCCGCCATCTTCATTCGGTATCTCCAGCTGGACCAACGGACAGGACATGCCGAGAAACTCAGCCACTGACATCAACATATCTTTACCAGTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

318

Amino Acids

33.15

Weight (kDa)

9.48

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 124 - 239 4.1e-26 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018289)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00200 AT4G00200
rosa_chinensis RchiOBHm_Chr4g0437561
rosa_roxburghii Rroxscaffold_2G00091570
rosa_rugosa Rorug01G0356200 Rorug02G0061400
rosa_samantha Rh1DG306100 Rh2AG329400 Rh6DG240300
rosa_wichuraiana Rw5G036080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 716
AccB7I CCANNNNNTGG 1 cut(s) 106
AccI GTMKAC 1 cut(s) 951
AciI CCGC 6 cut(s) 374, 513, 657, 681, 821, 863
AclI AACGTT 1 cut(s) 486
AclWI GGATC 1 cut(s) 367
AcoI YGGCCR 1 cut(s) 627
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 2 cut(s) 66, 132
AfaI GTAC 1 cut(s) 520
AfiI CCNNNNNNNGG 4 cut(s) 32, 106, 127, 149
AgsI TTSAA 1 cut(s) 594
AjnI CCWGG 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 130, 162
AloI GAACNNNNNNTCC 2 cut(s) 33, 65
AluBI AGCT 4 cut(s) 200, 262, 449, 888
AluI AGCT 4 cut(s) 200, 262, 449, 888
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 3 cut(s) 70, 120, 558
AlwI GGATC 1 cut(s) 367
AlwNI CAGNNNCTG 1 cut(s) 926
AoxI GGCC 2 cut(s) 627, 667
ApeKI GCWGC 2 cut(s) 776, 859
ApoI RAATTY 1 cut(s) 364
ArsI GACNNNNNNTTYG 2 cut(s) 587, 619
AspS9I GGNCC 3 cut(s) 440, 632, 891
AsuC2I CCSGG 1 cut(s) 848
AsuHPI GGTGA 4 cut(s) 329, 410, 517, 828
AvaII GGWCC 3 cut(s) 440, 632, 891
AxyI CCTNAGG 1 cut(s) 160
BauI CACGAG 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 2 cut(s) 788, 871
BccI CCATC 3 cut(s) 61, 632, 874
BciT130I CCWGG 1 cut(s) 27
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 1 cut(s) 848
BcoDI GTCTC 3 cut(s) 70, 120, 558
BfmI CTRYAG 1 cut(s) 774
BisI GCNGC 6 cut(s) 374, 681, 777, 822, 860, 863
BlsI GCNGC 6 cut(s) 375, 682, 778, 823, 861, 864
Bme1390I CCNGG 2 cut(s) 27, 848
Bme18I GGWCC 3 cut(s) 440, 632, 891
BmgT120I GGNCC 3 cut(s) 440, 632, 891
BmiI GGNNCC 2 cut(s) 72, 305
BmrFI CCNGG 2 cut(s) 27, 848
BmsI GCATC 1 cut(s) 276
BplI GAGNNNNNCTC 2 cut(s) 246, 278
BpmI CTGGAG 1 cut(s) 868
BpuMI CCSGG 1 cut(s) 848
BsaI GGTCTC 1 cut(s) 70
BsaJI CCNNGG 3 cut(s) 25, 126, 746
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 106, 127, 149
Bse118I RCCGGY 1 cut(s) 629
Bse1I ACTGG 3 cut(s) 100, 400, 947
Bse21I CCTNAGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 675
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 3 cut(s) 25, 126, 746
BseLI CCNNNNNNNGG 4 cut(s) 32, 106, 127, 149
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 2 cut(s) 174, 933
BseNI ACTGG 3 cut(s) 100, 400, 947
BseRI GAGGAG 6 cut(s) 187, 786, 789, 798, 801, 804
BseXI GCAGC 2 cut(s) 788, 871
BshFI GGCC 2 cut(s) 629, 669
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 3 cut(s) 630, 818, 848
BslI CCNNNNNNNGG 4 cut(s) 32, 106, 127, 149
BsmAI GTCTC 3 cut(s) 70, 120, 558
BsnI GGCC 2 cut(s) 629, 669
Bso31I GGTCTC 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 211
Bsp143I GATC 3 cut(s) 359, 724, 733
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 6 cut(s) 374, 513, 657, 681, 821, 863
BspANI GGCC 2 cut(s) 629, 669
BspCNI CTCAG 2 cut(s) 173, 932
BspLI GGNNCC 2 cut(s) 72, 305
BspMAI CTGCAG 1 cut(s) 778
BspPI GGATC 1 cut(s) 367
BspTNI GGTCTC 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 675
BsrFI RCCGGY 1 cut(s) 629
BsrI ACTGG 3 cut(s) 100, 400, 947
BssAI RCCGGY 1 cut(s) 629
BssECI CCNNGG 3 cut(s) 25, 126, 746
BssMI GATC 3 cut(s) 359, 724, 733
BssSI CACGAG 1 cut(s) 444
BssT1I CCWWGG 1 cut(s) 126
Bst2BI CACGAG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 388, 552
Bst6I CTCTTC 2 cut(s) 164, 498
BstC8I GCNNGC 4 cut(s) 260, 667, 671, 768
BstDEI CTNAG 2 cut(s) 160, 919
BstDSI CCRYGG 1 cut(s) 126
BstENI CCTNNNNNAGG 1 cut(s) 147
BstKTI GATC 3 cut(s) 362, 727, 736
BstMAI GTCTC 3 cut(s) 70, 120, 558
BstMBI GATC 3 cut(s) 359, 724, 733
BstMWI GCNNNNNNNGC 2 cut(s) 206, 776
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 3 cut(s) 380, 770, 910
BstSCI CCNGG 2 cut(s) 25, 846
BstSFI CTRYAG 1 cut(s) 774
BstV1I GCAGC 2 cut(s) 788, 871
BstXI CCANNNNNNTGG 1 cut(s) 314
Bsu36I CCTNAGG 1 cut(s) 160
BsuRI GGCC 2 cut(s) 629, 669
BtgI CCRYGG 1 cut(s) 126
BtsIMutI CAGTG 4 cut(s) 393, 393, 795, 924
Cac8I GCNNGC 4 cut(s) 260, 667, 671, 768
CaiI CAGNNNCTG 1 cut(s) 926
Cfr10I RCCGGY 1 cut(s) 629
Cfr13I GGNCC 3 cut(s) 440, 632, 891
Csp6I GTAC 1 cut(s) 519
CviAII CATG 7 cut(s) 127, 377, 571, 613, 767, 784, 907
CviQI GTAC 1 cut(s) 519
DdeI CTNAG 2 cut(s) 160, 919
DpnI GATC 3 cut(s) 361, 726, 735
DpnII GATC 3 cut(s) 359, 724, 733
EaeI YGGCCR 1 cut(s) 627
Eam1104I CTCTTC 2 cut(s) 164, 498
EarI CTCTTC 2 cut(s) 164, 498
Eco130I CCWWGG 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 70
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 3 cut(s) 440, 632, 891
Eco57I CTGAAG 2 cut(s) 66, 132
Eco81I CCTNAGG 1 cut(s) 160
EcoNI CCTNNNNNAGG 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 25
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 126
EcoT22I ATGCAT 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 7 cut(s) 130, 380, 574, 616, 770, 787, 910
FatI CATG 7 cut(s) 126, 376, 570, 612, 766, 783, 906
FauNDI CATATG 1 cut(s) 66
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 1 cut(s) 951
Fnu4HI GCNGC 6 cut(s) 374, 681, 777, 822, 860, 863
Fsp4HI GCNGC 6 cut(s) 374, 681, 777, 822, 860, 863
GluI GCNGC 6 cut(s) 374, 681, 777, 822, 860, 863
GsuI CTGGAG 1 cut(s) 868
HaeIII GGCC 2 cut(s) 629, 669
HapII CCGG 3 cut(s) 630, 818, 848
Hin1II CATG 7 cut(s) 130, 380, 574, 616, 770, 787, 910
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HinfI GANTC 4 cut(s) 417, 506, 583, 759
HpaII CCGG 3 cut(s) 630, 818, 848
HphI GGTGA 4 cut(s) 329, 410, 517, 828
Hpy166II GTNNAC 2 cut(s) 391, 952
Hpy188I TCNGA 3 cut(s) 75, 85, 741
Hpy188III TCNNGA 1 cut(s) 635
Hpy8I GTNNAC 2 cut(s) 391, 952
HpyAV CCTTC 4 cut(s) 90, 153, 228, 813
HpyCH4III ACNGT 2 cut(s) 388, 552
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 2 cut(s) 289, 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 776
HpyF3I CTNAG 2 cut(s) 160, 919
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 7 cut(s) 130, 380, 574, 616, 770, 787, 910
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 3 cut(s) 359, 724, 733
LmnI GCTCC 3 cut(s) 41, 76, 214
Lsp1109I GCAGC 2 cut(s) 788, 871
LweI GCATC 1 cut(s) 276
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 487
MalI GATC 3 cut(s) 361, 726, 735
MboI GATC 3 cut(s) 359, 724, 733
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 2 cut(s) 18, 364
MlyI GAGTC 1 cut(s) 768
MmeI TCCRAC 1 cut(s) 337
Mph1103I ATGCAT 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 788
MspA1I CMGCKG 1 cut(s) 888
MspI CCGG 3 cut(s) 630, 818, 848
MspR9I CCNGG 2 cut(s) 27, 848
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 206, 776
NciI CCSGG 1 cut(s) 848
NcoI CCATGG 1 cut(s) 126
NdeI CATATG 1 cut(s) 66
NdeII GATC 3 cut(s) 359, 724, 733
NlaIII CATG 7 cut(s) 130, 380, 574, 616, 770, 787, 910
NlaIV GGNNCC 2 cut(s) 72, 305
NmeAIII GCCGAG 1 cut(s) 936
NsiI ATGCAT 1 cut(s) 291
NspI RCATGY 3 cut(s) 380, 770, 910
PaeI GCATGC 1 cut(s) 770
PfeI GAWTC 3 cut(s) 417, 506, 583
PflMI CCANNNNNTGG 1 cut(s) 106
PkrI GCNGC 6 cut(s) 375, 682, 778, 823, 861, 864
PleI GAGTC 1 cut(s) 767
PpsI GAGTC 1 cut(s) 767
PsiI TTATAA 1 cut(s) 716
Psp1406I AACGTT 1 cut(s) 486
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 2 cut(s) 72, 305
PspPI GGNCC 3 cut(s) 440, 632, 891
PstI CTGCAG 1 cut(s) 778
PstNI CAGNNNCTG 1 cut(s) 926
PvuII CAGCTG 1 cut(s) 888
RsaI GTAC 1 cut(s) 520
RsaNI GTAC 1 cut(s) 519
RseI CAYNNNNRTG 1 cut(s) 788
SalI GTCGAC 1 cut(s) 950
SatI GCNGC 6 cut(s) 374, 681, 777, 822, 860, 863
Sau3AI GATC 3 cut(s) 359, 724, 733
Sau96I GGNCC 3 cut(s) 440, 632, 891
SchI GAGTC 1 cut(s) 768
ScrFI CCNGG 2 cut(s) 27, 848
SduI GDGCHC 1 cut(s) 211
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 1 cut(s) 774
SinI GGWCC 3 cut(s) 440, 632, 891
SmiMI CAYNNNNRTG 1 cut(s) 788
SphI GCATGC 1 cut(s) 770
Sse9I AATT 2 cut(s) 18, 364
SsiI CCGC 6 cut(s) 374, 513, 657, 681, 821, 863
StyD4I CCNGG 2 cut(s) 25, 846
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 388, 552
TaiI ACGT 1 cut(s) 489
TaqI TCGA 2 cut(s) 762, 951
TaqII GACCGA 1 cut(s) 591
TasI AATT 2 cut(s) 18, 364
TauI GCSGC 4 cut(s) 376, 683, 824, 865
TfiI GAWTC 3 cut(s) 417, 506, 583
TscAI CASTG 4 cut(s) 393, 400, 795, 931
TseI GCWGC 2 cut(s) 776, 859
TspDTI ATGAA 6 cut(s) 210, 213, 293, 555, 559, 861
TspGWI ACGGA 1 cut(s) 912
TspRI CASTG 4 cut(s) 393, 400, 795, 931
Van91I CCANNNNNTGG 1 cut(s) 106
VpaK11BI GGWCC 3 cut(s) 440, 632, 891
XagI CCTNNNNNAGG 1 cut(s) 147
XapI RAATTY 1 cut(s) 364
XceI RCATGY 3 cut(s) 380, 770, 910
XmiI GTMKAC 1 cut(s) 951
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.