Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0013057

Physical Location & Seq

Genomic Coordinates Reverse (-)

242461 .. 246804

Gene Structure 4344 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G00570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1824 bp

ATGATGTGGAAGAACATTGCTGGGTTGTCGAAGGCAGCGGCAGCGGCAAGAACACACGGATCTCGGCGGTGCTTTTCCACAGCGATTCCTGGTCCTTGCATCGTCCACAAGCGTGGTGCTGATATTCTTCACGATCCATGGTTCAATAAGGACACAGGTTTTCCTTTGACTGAGAGAGATAGATTAGGGATACGAGGCTTGCTTCCTCCTCGTGTTATGACCTGTGTACAGCAATGTGATCGTTTCATTGAGTCGTTTCGTTCGCTAGAGAACAACACAAAAGGAGAGCCTGAAAACGTTGTTGCATTAGCTAAATGGAGGATGTTGAATAGATTGCATGACCGTAATGAGACTTTGTACTACCGAGTTCTTATTGATAACATCAAAGATTTTGCCCCCATTATATACACTCCTACGGTTGGACTTGTTTGTCAGAACTATTCTGGTTTGTATAGAAGACCAAGAGGAATGTACTTCAGCGCCAAAGACAAAGGAGAGATGATGTCTATGATTTATAACTGGCCTGCTCCGCAGGTAGATATGATTGTCATCACTGATGGTAGCCGTATTTTGGGCTTAGGAGACCTTGGAGTACAGGGAATTGGGATTCCTATTGGAAAGCTCGACATGTATGTCGCTGCTGCAGGAATCAATCCACAGAGAGTTTTGCCAATTATGTTGGATGTAGGCACAAATAATGAAAAGCTCCTACAGAATGATCTCTATTTAGGAGTCAGACAACCTAGGTTGGAAGGAGAGGAGTACCTTGAGATTATTGATGAATTTATGGAAGCTGCATTTACACGTTGGCCAAAGGCCGTCGTACAGTTCGAAGATTTCCAAGCGAAATGGGCTTTCGGAACATTGGAAAGATATCGGAAGAAATTTTGCATGTTCAATGATGATGTCCAGGGAACTGCTGGTGTTGCACTGGCTGGACTATTGGGAACTGTAAGAGCTCAAGGTCGGCCGATATCCGACTTTGTGAACCAAAAGATTGTTGTGGTGGGAGCTGGAAGTGCTGGGCTTGGTGTAACTAAGATGGCAGTACAGGCAGTTGCCAGAATGGCAGGTATCAGCGAGAGTGAAGCAACAAAAAACTTTTACCTGATAGATAAAGATGGTCTTGTCACCACGGAGAGGACAAAACTTGACCCAGGAGCTGTACTTTTTGCCAAAAATCCAGCTGAGATACGCGAGGGAGCAAGTATCGTTGAAGTGGTGAAGAAAGTGAGGCCACATGTTCTTCTTGGTTTATCTGGAGTTGGTGGTATCTTTAATGAAGAAGTTCTCAAGGCGATGCGAGAATCTGACTCATGCAAGCCTGCCATTTTTGCCATGTCGAATCCCACTTTGAATGCCGAGTGCACTGCTGCTGATGCTTTCAAACACGCCGGAGGAAACATTGTCTTTGCAAGTGGAAGTCCATTTGAGAACGTTGAACTTGAGAATGGTAAAGTAGGACATGTGAATCAGGCTAACAACATGTATTTATTCCCGGGGATCGGGCTAGGGACTCTTCTCTCTGGTGCACGTATCGTAACTGATGGAATGCTACAAGCAGCTTCTGAATGCCTTGCGTCCTACATGACCGATGAAGAAGTCCAAAAAGGCATCCTTTACCCTTCAATCAACAACATCCGACACATAACAGCTGAAGTCGGGGCAGCTGTTCTACGAGCTGCGGTGACTGATGACATTGCAGAAGGACATGGTGATGTTGGTCCCAAAGATCTCAGCCACATGTCTAAGGAGGATACAGTGAATTACATTACACGCAACATGTGGTTCCCTGTTTACAGCCCTCTCGTTCACGAGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004470 GO:0004471 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006090 GO:0006108 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0017076 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0032787 GO:0035639 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046983 GO:0050897 GO:0055114 GO:0070013 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

607

Amino Acids

66.64

Weight (kDa)

6.63

Isoelectric Point (pI)

32.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	113 - 293	1.2e-74	Malic enzyme, N-terminal domain
Malic_M	PF03949	303 - 563	1.9e-90	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013057)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00570
fragaria_vesca	FvH4_7g13590
malus_domestica	MD01G1052400.v1.1 MD07G1129000.v1.1
prunus_persica	Prupe.2G160000_v2.0.a1 Prupe.2G160000_v2.0.a1 Prupe.2G160000_v2.0.a1
pyrus_communis	pycom01g07810 pycom07g12470
rosa_chinensis	RchiOBHm_Chr1g0353381
rosa_laevigata	RLG00000028273
rosa_multiflora	Rmu_sc0001207.1_g000013
rosa_roxburghii	Rroxscaffold_4G00301880
rosa_rugosa	Rorug01G0235600
rosa_samantha	Rh1AG247800 Rh1BG217600 Rh1CG231500
rosa_wichuraiana	Rw1G021590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	516
AasI	GACNNNNNNGTC	2 cut(s)	429, 632
Acc36I	ACCTGC	2 cut(s)	523, 1061
AccII	CGCG	1 cut(s)	1197
AciI	CCGC	5 cut(s)	38, 44, 67, 530, 1685
AclI	AACGTT	2 cut(s)	297, 1437
AclWI	GGATC	3 cut(s)	67, 128, 1511
AcoI	YGGCCR	2 cut(s)	809, 968
AcsI	RAATTY	2 cut(s)	782, 884
AcuI	CTGAAG	2 cut(s)	460, 1677
AfaI	GTAC	8 cut(s)	228, 359, 473, 594, 764, 825, 1050, 1167
AfiI	CCNNNNNNNGG	4 cut(s)	419, 571, 1140, 1505
AflIII	ACRYGT	7 cut(s)	627, 803, 1240, 1465, 1485, 1743, 1782
AgsI	TTSAA	8 cut(s)	145, 328, 898, 1217, 1357, 1387, 1442, 1629
AjnI	CCWGG	3 cut(s)	88, 909, 1156
AleI	CACNNNNGTG	1 cut(s)	111
Alw21I	GWGCWC	3 cut(s)	961, 1370, 1534
Alw26I	GTCTC	2 cut(s)	344, 576
Alw44I	GTGCAC	2 cut(s)	1366, 1530
AlwI	GGATC	3 cut(s)	67, 128, 1511
AlwNI	CAGNNNCTG	2 cut(s)	1163, 1568
Ama87I	CYCGRG	1 cut(s)	1498
AoxI	GGCC	5 cut(s)	521, 809, 816, 968, 1235
ApaLI	GTGCAC	2 cut(s)	1366, 1530
ApeKI	GCWGC	9 cut(s)	35, 41, 638, 641, 794, 1373, 1562, 1667, 1682
ApoI	RAATTY	2 cut(s)	782, 884
Asp700I	GAANNNNTTC	1 cut(s)	1287
AspA2I	CCTAGG	1 cut(s)	743
AspLEI	GCGC	1 cut(s)	482
AspS9I	GGNCC	2 cut(s)	92, 1724
AsuC2I	CCSGG	2 cut(s)	1499, 1500
AsuHPI	GGTGA	4 cut(s)	1123, 1234, 1699, 1727
AsuII	TTCGAA	1 cut(s)	831
AvaI	CYCGRG	1 cut(s)	1498
AvaII	GGWCC	2 cut(s)	92, 1724
AvrII	CCTAGG	1 cut(s)	743
BaeGI	GKGCMC	2 cut(s)	1370, 1534
BalI	TGGCCA	1 cut(s)	811
BanII	GRGCYC	1 cut(s)	961
BauI	CACGAG	2 cut(s)	210, 1814
BbsI	GAAGAC	1 cut(s)	463
Bbv12I	GWGCWC	3 cut(s)	961, 1370, 1534
BbvI	GCAGC	9 cut(s)	47, 53, 625, 628, 781, 1360, 1574, 1669, 1679
BccI	CCATC	4 cut(s)	551, 1036, 1115, 1541
BceAI	ACGGC	2 cut(s)	549, 803
BcgI	CGANNNNNNTGC	4 cut(s)	221, 255, 1352, 1386
BciT130I	CCWGG	3 cut(s)	90, 911, 1158
BciVI	GTATCC	2 cut(s)	183, 1750
BcnI	CCSGG	2 cut(s)	1499, 1500
BcoDI	GTCTC	2 cut(s)	344, 576
BfaI	CTAG	3 cut(s)	266, 744, 1511
BfmI	CTRYAG	2 cut(s)	642, 710
BfoI	RGCGCY	1 cut(s)	483
BfuAI	ACCTGC	2 cut(s)	523, 1061
BfuI	GTATCC	2 cut(s)	183, 1750
BglI	GCCNNNNNGGC	1 cut(s)	1067
BglII	AGATCT	1 cut(s)	1732
BlnI	CCTAGG	1 cut(s)	743
Bme1390I	CCNGG	5 cut(s)	90, 911, 1158, 1499, 1500
Bme18I	GGWCC	2 cut(s)	92, 1724
BmeT110I	CYCGRG	1 cut(s)	1498
BmgT120I	GGNCC	2 cut(s)	92, 1724
BmiI	GGNNCC	2 cut(s)	1726, 1790
BmrFI	CCNGG	5 cut(s)	90, 911, 1158, 1499, 1500
BmsI	GCATC	4 cut(s)	108, 1290, 1369, 1623
BpiI	GAAGAC	1 cut(s)	463
BpmI	CTGGAG	1 cut(s)	1281
Bpu10I	CCTNAGC	1 cut(s)	577
Bpu14I	TTCGAA	1 cut(s)	831
BpuEI	CTTGAG	4 cut(s)	788, 945, 1277, 1466
BpuMI	CCSGG	2 cut(s)	1499, 1500
BsaAI	YACGTR	1 cut(s)	1535
BsaBI	GATNNNNATC	1 cut(s)	548
BsaI	GGTCTC	1 cut(s)	576
BsaJI	CCNNGG	8 cut(s)	137, 586, 743, 910, 1134, 1156, 1498, 1499
Bsc4I	CCNNNNNNNGG	4 cut(s)	419, 571, 1140, 1505
Bse1I	ACTGG	2 cut(s)	524, 936
Bse3DI	GCAATG	3 cut(s)	15, 239, 1698
Bse8I	GATNNNNATC	1 cut(s)	548
BseBI	CCWGG	3 cut(s)	90, 911, 1158
BseDI	CCNNGG	8 cut(s)	137, 586, 743, 910, 1134, 1156, 1498, 1499
BseGI	GGATG	4 cut(s)	327, 688, 1614, 1638
BseJI	GATNNNNATC	1 cut(s)	548
BseLI	CCNNNNNNNGG	4 cut(s)	419, 571, 1140, 1505
BseMI	GCAATG	3 cut(s)	15, 239, 1698
BseMII	CTCAG	3 cut(s)	162, 1179, 1750
BseNI	ACTGG	2 cut(s)	524, 936
BseRI	GAGGAG	2 cut(s)	198, 773
BseSI	GKGCMC	2 cut(s)	1370, 1534
BseX3I	CGGCCG	1 cut(s)	968
BseXI	GCAGC	9 cut(s)	47, 53, 625, 628, 781, 1360, 1574, 1669, 1679
BseYI	CCCAGC	2 cut(s)	20, 1022
Bsh1236I	CGCG	1 cut(s)	1197
Bsh1285I	CGRYCG	1 cut(s)	971
BshFI	GGCC	5 cut(s)	523, 811, 818, 970, 1237
BsiEI	CGRYCG	1 cut(s)	971
BsiHKAI	GWGCWC	3 cut(s)	961, 1370, 1534
BsiHKCI	CYCGRG	1 cut(s)	1498
BsiSI	CCGG	2 cut(s)	1395, 1499
BslFI	GGGAC	2 cut(s)	1528, 1710
BslI	CCNNNNNNNGG	4 cut(s)	419, 571, 1140, 1505
BsmAI	GTCTC	2 cut(s)	344, 576
BsmFI	GGGAC	2 cut(s)	1528, 1710
BsmI	GAATGC	3 cut(s)	1363, 1557, 1577
BsnI	GGCC	5 cut(s)	523, 811, 818, 970, 1237
Bso31I	GGTCTC	1 cut(s)	576
BsoBI	CYCGRG	1 cut(s)	1498
Bsp119I	TTCGAA	1 cut(s)	831
Bsp1286I	GDGCHC	3 cut(s)	961, 1370, 1534
Bsp1407I	TGTACA	1 cut(s)	226
Bsp143I	GATC	6 cut(s)	59, 133, 238, 718, 1503, 1732
Bsp19I	CCATGG	1 cut(s)	137
BspACI	CCGC	5 cut(s)	38, 44, 67, 530, 1685
BspANI	GGCC	5 cut(s)	523, 811, 818, 970, 1237
BspCNI	CTCAG	3 cut(s)	163, 1180, 1749
BspFNI	CGCG	1 cut(s)	1197
BspLI	GGNNCC	2 cut(s)	1726, 1790
BspMAI	CTGCAG	1 cut(s)	646
BspMI	ACCTGC	2 cut(s)	523, 1061
BspPI	GGATC	3 cut(s)	67, 128, 1511
BspT104I	TTCGAA	1 cut(s)	831
BspTNI	GGTCTC	1 cut(s)	576
BsrDI	GCAATG	3 cut(s)	15, 239, 1698
BsrGI	TGTACA	1 cut(s)	226
BsrI	ACTGG	2 cut(s)	524, 936
BssECI	CCNNGG	8 cut(s)	137, 586, 743, 910, 1134, 1156, 1498, 1499
BssMI	GATC	6 cut(s)	59, 133, 238, 718, 1503, 1732
BssSI	CACGAG	2 cut(s)	210, 1814
BssT1I	CCWWGG	3 cut(s)	137, 586, 743
Bst2BI	CACGAG	2 cut(s)	210, 1814
Bst2UI	CCWGG	3 cut(s)	90, 911, 1158
Bst4CI	ACNGT	5 cut(s)	344, 418, 828, 952, 1762
Bst6I	CTCTTC	1 cut(s)	1524
BstAUI	TGTACA	1 cut(s)	226
BstBAI	YACGTR	1 cut(s)	1535
BstBI	TTCGAA	1 cut(s)	831
BstC8I	GCNNGC	4 cut(s)	200, 525, 1322, 1326
BstDEI	CTNAG	6 cut(s)	171, 577, 1038, 1188, 1736, 1749
BstDSI	CCRYGG	2 cut(s)	137, 1134
BstF5I	GGATG	4 cut(s)	327, 688, 1614, 1638
BstFNI	CGCG	1 cut(s)	1197
BstH2I	RGCGCY	1 cut(s)	483
BstHHI	GCGC	1 cut(s)	482
BstKTI	GATC	6 cut(s)	62, 136, 241, 721, 1506, 1735
BstMAI	GTCTC	2 cut(s)	344, 576
BstMBI	GATC	6 cut(s)	59, 133, 238, 718, 1503, 1732
BstMCI	CGRYCG	1 cut(s)	971
BstNI	CCWGG	3 cut(s)	90, 911, 1158
BstNSI	RCATGY	7 cut(s)	631, 895, 1244, 1469, 1489, 1747, 1786
BstSCI	CCNGG	5 cut(s)	88, 909, 1156, 1497, 1498
BstSFI	CTRYAG	2 cut(s)	642, 710
BstSLI	GKGCMC	2 cut(s)	1370, 1534
BstUI	CGCG	1 cut(s)	1197
BstV1I	GCAGC	9 cut(s)	47, 53, 625, 628, 781, 1360, 1574, 1669, 1679
BstV2I	GAAGAC	1 cut(s)	463
BstX2I	RGATCY	2 cut(s)	59, 1732
BstXI	CCANNNNNNTGG	1 cut(s)	113
BstYI	RGATCY	2 cut(s)	59, 1732
BstZI	CGGCCG	1 cut(s)	968
BsuI	GTATCC	2 cut(s)	183, 1750
BsuRI	GGCC	5 cut(s)	523, 811, 818, 970, 1237
BtgI	CCRYGG	2 cut(s)	137, 1134
BtgZI	GCGATG	1 cut(s)	1313
BtsCI	GGATG	4 cut(s)	327, 688, 1614, 1638
BtsI	GCAGTG	1 cut(s)	1368
BtsIMutI	CAGTG	4 cut(s)	552, 929, 1368, 1767
BveI	ACCTGC	2 cut(s)	523, 1061
Cac8I	GCNNGC	4 cut(s)	200, 525, 1322, 1326
CaiI	CAGNNNCTG	2 cut(s)	1163, 1568
CfoI	GCGC	1 cut(s)	482
Cfr13I	GGNCC	2 cut(s)	92, 1724
Cfr9I	CCCGGG	1 cut(s)	1498
CseI	GACGC	1 cut(s)	1569
Csp6I	GTAC	8 cut(s)	227, 358, 472, 593, 763, 824, 1049, 1166
CviQI	GTAC	8 cut(s)	227, 358, 472, 593, 763, 824, 1049, 1166
DdeI	CTNAG	6 cut(s)	171, 577, 1038, 1188, 1736, 1749
DpnI	GATC	6 cut(s)	61, 135, 240, 720, 1505, 1734
DpnII	GATC	6 cut(s)	59, 133, 238, 718, 1503, 1732
DrdI	GACNNNNNNGTC	2 cut(s)	429, 632
DseDI	GACNNNNNNGTC	2 cut(s)	429, 632
EaeI	YGGCCR	2 cut(s)	809, 968
EagI	CGGCCG	1 cut(s)	968
Eam1104I	CTCTTC	1 cut(s)	1524
EarI	CTCTTC	1 cut(s)	1524
Ecl136II	GAGCTC	1 cut(s)	959
EclXI	CGGCCG	1 cut(s)	968
Eco130I	CCWWGG	3 cut(s)	137, 586, 743
Eco24I	GRGCYC	1 cut(s)	961
Eco31I	GGTCTC	1 cut(s)	576
Eco32I	GATATC	2 cut(s)	875, 975
Eco47I	GGWCC	2 cut(s)	92, 1724
Eco52I	CGGCCG	1 cut(s)	968
Eco53kI	GAGCTC	1 cut(s)	959
Eco57I	CTGAAG	2 cut(s)	460, 1677
Eco88I	CYCGRG	1 cut(s)	1498
EcoICRI	GAGCTC	1 cut(s)	959
EcoRII	CCWGG	3 cut(s)	88, 909, 1156
EcoRV	GATATC	2 cut(s)	875, 975
EcoT14I	CCWWGG	3 cut(s)	137, 586, 743
EcoT38I	GRGCYC	1 cut(s)	961
ErhI	CCWWGG	3 cut(s)	137, 586, 743
FalI	AAGNNNNNCTT	4 cut(s)	1110, 1142, 1602, 1634
FaqI	GGGAC	2 cut(s)	1528, 1710
FokI	GGATG	4 cut(s)	334, 695, 1601, 1625
FriOI	GRGCYC	1 cut(s)	961
FspBI	CTAG	3 cut(s)	266, 744, 1511
GlaI	GCGC	1 cut(s)	481
GsaI	CCCAGC	2 cut(s)	24, 1026
GsuI	CTGGAG	1 cut(s)	1281
HaeII	RGCGCY	1 cut(s)	483
HaeIII	GGCC	5 cut(s)	523, 811, 818, 970, 1237
HapII	CCGG	2 cut(s)	1395, 1499
HgaI	GACGC	1 cut(s)	1569
HhaI	GCGC	1 cut(s)	482
Hin6I	GCGC	1 cut(s)	480
HinP1I	GCGC	1 cut(s)	480
HpaII	CCGG	2 cut(s)	1395, 1499
HphI	GGTGA	4 cut(s)	1123, 1234, 1699, 1727
Hpy166II	GTNNAC	7 cut(s)	106, 227, 988, 1368, 1532, 1798, 1813
Hpy188I	TCNGA	8 cut(s)	435, 737, 860, 879, 979, 1312, 1570, 1643
Hpy188III	TCNNGA	3 cut(s)	131, 1260, 1814
Hpy8I	GTNNAC	7 cut(s)	106, 227, 988, 1368, 1532, 1798, 1813
Hpy99I	CGWCG	1 cut(s)	824
HpyAV	CCTTC	4 cut(s)	25, 746, 1635, 1700
HpyCH4III	ACNGT	5 cut(s)	344, 418, 828, 952, 1762
HpyCH4IV	ACGT	4 cut(s)	297, 805, 1437, 1534
HpyF3I	CTNAG	6 cut(s)	171, 577, 1038, 1188, 1736, 1749
HpySE526I	ACGT	4 cut(s)	297, 805, 1437, 1534
HspAI	GCGC	1 cut(s)	480
Kzo9I	GATC	6 cut(s)	59, 133, 238, 718, 1503, 1732
LmnI	GCTCC	5 cut(s)	532, 711, 1010, 1160, 1202
Lsp1109I	GCAGC	9 cut(s)	47, 53, 625, 628, 781, 1360, 1574, 1669, 1679
LweI	GCATC	4 cut(s)	108, 1290, 1369, 1623
MaeI	CTAG	3 cut(s)	266, 744, 1511
MaeII	ACGT	4 cut(s)	297, 805, 1437, 1534
MaeIII	GTNAC	4 cut(s)	1033, 1129, 1540, 1687
MalI	GATC	6 cut(s)	61, 135, 240, 720, 1505, 1734
MboI	GATC	6 cut(s)	59, 133, 238, 718, 1503, 1732
MflI	RGATCY	2 cut(s)	59, 1732
MhlI	GDGCHC	3 cut(s)	961, 1370, 1534
MlsI	TGGCCA	1 cut(s)	811
MluCI	AATT	5 cut(s)	600, 672, 782, 884, 1765
MluNI	TGGCCA	1 cut(s)	811
MlyI	GAGTC	4 cut(s)	260, 741, 1307, 1510
MmeI	TCCRAC	5 cut(s)	400, 660, 729, 1002, 1666
Mox20I	TGGCCA	1 cut(s)	811
MroXI	GAANNNNTTC	1 cut(s)	1287
MscI	TGGCCA	1 cut(s)	811
MseI	TTAA	1 cut(s)	1278
MslI	CAYNNNNRTG	2 cut(s)	111, 1716
Msp20I	TGGCCA	1 cut(s)	811
MspA1I	CMGCKG	5 cut(s)	38, 44, 1187, 1655, 1670
MspI	CCGG	2 cut(s)	1395, 1499
MspR9I	CCNGG	5 cut(s)	90, 911, 1158, 1499, 1500
Mva1269I	GAATGC	3 cut(s)	1363, 1557, 1577
MvaI	CCWGG	3 cut(s)	90, 911, 1158
MvnI	CGCG	1 cut(s)	1197
NciI	CCSGG	2 cut(s)	1499, 1500
NcoI	CCATGG	1 cut(s)	137
NdeII	GATC	6 cut(s)	59, 133, 238, 718, 1503, 1732
NlaIV	GGNNCC	2 cut(s)	1726, 1790
NmeAIII	GCCGAG	2 cut(s)	43, 1387
NmuCI	GTSAC	2 cut(s)	1129, 1687
NspI	RCATGY	7 cut(s)	631, 895, 1244, 1469, 1489, 1747, 1786
NspV	TTCGAA	1 cut(s)	831
OliI	CACNNNNGTG	1 cut(s)	111
PciI	ACATGT	6 cut(s)	627, 1240, 1465, 1485, 1743, 1782
PcsI	WCGNNNNNNNCGW	1 cut(s)	828
PctI	GAATGC	3 cut(s)	1363, 1557, 1577
PdmI	GAANNNNTTC	1 cut(s)	1287
PfeI	GAWTC	6 cut(s)	85, 607, 648, 1307, 1345, 1471
PleI	GAGTC	4 cut(s)	259, 740, 1307, 1510
PpsI	GAGTC	4 cut(s)	259, 740, 1307, 1510
Ppu21I	YACGTR	1 cut(s)	1535
PscI	ACATGT	6 cut(s)	627, 1240, 1465, 1485, 1743, 1782
PsiI	TTATAA	1 cut(s)	516
Psp124BI	GAGCTC	1 cut(s)	961
Psp1406I	AACGTT	2 cut(s)	297, 1437
Psp6I	CCWGG	3 cut(s)	88, 909, 1156
PspFI	CCCAGC	2 cut(s)	20, 1022
PspGI	CCWGG	3 cut(s)	88, 909, 1156
PspN4I	GGNNCC	2 cut(s)	1726, 1790
PspPI	GGNCC	2 cut(s)	92, 1724
PstI	CTGCAG	1 cut(s)	646
PstNI	CAGNNNCTG	2 cut(s)	1163, 1568
PsuI	RGATCY	2 cut(s)	59, 1732
PvuII	CAGCTG	3 cut(s)	1187, 1655, 1670
RsaI	GTAC	8 cut(s)	228, 359, 473, 594, 764, 825, 1050, 1167
RsaNI	GTAC	8 cut(s)	227, 358, 472, 593, 763, 824, 1049, 1166
RseI	CAYNNNNRTG	2 cut(s)	111, 1716
SacI	GAGCTC	1 cut(s)	961
SaqAI	TTAA	1 cut(s)	1278
Sau3AI	GATC	6 cut(s)	59, 133, 238, 718, 1503, 1732
Sau96I	GGNCC	2 cut(s)	92, 1724
SchI	GAGTC	4 cut(s)	260, 741, 1307, 1510
ScrFI	CCNGG	5 cut(s)	90, 911, 1158, 1499, 1500
SduI	GDGCHC	3 cut(s)	961, 1370, 1534
SfaNI	GCATC	4 cut(s)	108, 1290, 1369, 1623
SfcI	CTRYAG	2 cut(s)	642, 710
SfuI	TTCGAA	1 cut(s)	831
SinI	GGWCC	2 cut(s)	92, 1724
SmaI	CCCGGG	1 cut(s)	1500
SmiMI	CAYNNNNRTG	2 cut(s)	111, 1716
SmlI	CTYRAG	4 cut(s)	767, 960, 1292, 1445
SmoI	CTYRAG	4 cut(s)	767, 960, 1292, 1445
Sse9I	AATT	5 cut(s)	600, 672, 782, 884, 1765
SsiI	CCGC	5 cut(s)	38, 44, 67, 530, 1685
SspMI	CTAG	3 cut(s)	266, 744, 1511
SstI	GAGCTC	1 cut(s)	961
StyD4I	CCNGG	5 cut(s)	88, 909, 1156, 1497, 1498
StyI	CCWWGG	3 cut(s)	137, 586, 743
TaaI	ACNGT	5 cut(s)	344, 418, 828, 952, 1762
TaiI	ACGT	4 cut(s)	300, 808, 1440, 1537
TaqI	TCGA	4 cut(s)	29, 624, 831, 1343
TaqII	GACCGA	1 cut(s)	1607
TasI	AATT	5 cut(s)	600, 672, 782, 884, 1765
TatI	WGTACW	6 cut(s)	226, 357, 471, 592, 1048, 1165
TauI	GCSGC	2 cut(s)	41, 47
TfiI	GAWTC	6 cut(s)	85, 607, 648, 1307, 1345, 1471
Tru1I	TTAA	1 cut(s)	1278
Tru9I	TTAA	1 cut(s)	1278
TscAI	CASTG	4 cut(s)	559, 936, 1375, 1767
TseFI	GTSAC	2 cut(s)	1129, 1687
TseI	GCWGC	9 cut(s)	35, 41, 638, 641, 794, 1373, 1562, 1667, 1682
Tsp45I	GTSAC	2 cut(s)	1129, 1687
TspDTI	ATGAA	5 cut(s)	235, 714, 795, 1296, 1611
TspGWI	ACGGA	2 cut(s)	72, 1151
TspMI	CCCGGG	1 cut(s)	1498
TspRI	CASTG	4 cut(s)	559, 936, 1375, 1767
VneI	GTGCAC	2 cut(s)	1366, 1530
VpaK11BI	GGWCC	2 cut(s)	92, 1724
XapI	RAATTY	2 cut(s)	782, 884
XceI	RCATGY	7 cut(s)	631, 895, 1244, 1469, 1489, 1747, 1786
XcmI	CCANNNNNNNNNTGG	1 cut(s)	917
XmaI	CCCGGG	1 cut(s)	1498
XmaJI	CCTAGG	1 cut(s)	743
XmnI	GAANNNNTTC	1 cut(s)	1287
XspI	CTAG	3 cut(s)	266, 744, 1511

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.