Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0013057

Physical Location & Seq

Genomic Coordinates Forward (+)

18413232 .. 18420026

Gene Structure 6795 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1129000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGTGGAAGGCAGCGCGATTCGCCGCCTCGCAGTTGAGCAGCCGGTCGACGAGGCGGTTCTCGACGGCGATTCCCGCCCCCTGTATCGTTCACAAGCGCGGCGCCGATATTCTCCACGATCCATGGTTTAACAAGCAGGACACTGGATTTCCGTTGACTGAAAGAGATCGATTAGGACTTCGCGGTCTCCTCCCACCTCGTGTCATATCATTCGAGCAGCAATATGCTCGTTTCATGGAGTCCTATCGTTCCCTAGAGAAAAATACTAAGGGCCAGCCAGAGGGTGTTGTGGCCTTGGCTAAATGGAGGATCTTAAATAGACTGCATGACAGGAACGAGACCTTGTACTACCGTGTCCTTATTGACAACATCCAAGATTTTGCTCCCATAATTTACACTCCTACAGTAGGATTAGTATGTCAAAATTATTCTGGTTTATTTAGACGTCCCCGTGGAATGTATTTCAGTGCAAAGGATAAAGGGGAGATGATGTCTATGATCTACAACTGGCCAGCACATCAGGTAGACATGATTGTCCTCACAGATGGCAGTCGTATCCTTGGCCTAGGGGACCTTGGAGTTCAGGGAATTGGAATTCCAATTGGAAAACTTGATATGTATGTTGCTGCAGCTGGTATTAATCCACAAAGAATACTACCGGTTATGCTAGATGTTGGTACTAACAACCAAAAGCTACTTGAAGACCGTCTTTATTTAGGACTTCGACAACCTAGGTTGGAAGGAGAAGAGTACATATCAATTGTTGATGAATTCATGGAAGCTGTTCATACACGTTGGCCCAAGGCCATTGTGCAGTTTGAGGATTTTCAAATGAAGTGGGCTTTTGAAACACTGCAACGCTATCGTAAAAGGTTTTGCACGTTCAATGATGACATACAGGGAACTGCCGGTGTTGCACTTGCAGGACTATTAGGAACTGTAAGAGTGCAAGGTCGACCATTGAGTGACTTTGCTAAACAAAAGATAGTTGTTGTGGGAGCCGGAAGTGCGGGACTTGGAGTTCTTAACATGGCTGTACAGGCTGTTGCAAGAATGTCAGGGAACGGTGAAGCTGCTGCAAAAAATAATTTTTTCCTAATTGATAAAGATGGTCTAGTTACAAAAGAGAGAAATAATCTTGACCCCATGGCTGCACCCTTTGCTAAAGAACCGGGAGCAATTGATGGACTTAGGGAGGGAGCTAGTCTACTTGAAGTGGTTAAGAAGGTCAAGCCACATGTGCTTCTTGGTTTGTCTGGAGTTGGTGGTGTTTTCAATCATGAGGTACTTAAAGCAATGCAAGAATCAGATTCAACTAAACCTGCCATTTTTGCTATGTCAAATCCAACCATGAATGCTGAATGCACTGCTGAAGATGCTTTTAAGCATGCTGGTGAAAGCATCGTCTTTGGAAGTGGAAGCCCCTTTGATAATGTTGACCTTGGCAACGGAAAAGTAGGCCATGTAAATCAAGCAAATAACATGTATCTGTTTCCAGGGATTGGTTTAGGAGCACTTCTCTCAGGAGCTCGTATTATATCAGATGGCATGTTACAAGCAGCTTCTGAATGCCTCGCTTCATACATAACAGATGTAGATATCCAGAAGGGCATCTTGTACCCATCTATTGATTGTATCCGGAATATAACCGCGGAGGTTGGAGCTGCTGTCCTGCGGGCAGCTGTTGCAGAAGAACTGGCAGAGGGACACTGTGAAGTTGGGCCCAAAGAGCTCATGAACATGTGCAAAGAGGAGACTGTGGAGTATGTGAGTCGCAGTATGTGGTTCCCGGTTTACAGCCCTCTAGTTCACGAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004470 GO:0004471 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006090 GO:0006108 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0017076 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0032787 GO:0035639 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046983 GO:0050897 GO:0055114 GO:0070013 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

607

Amino Acids

66.77

Weight (kDa)

6.85

Isoelectric Point (pI)

33.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	109 - 289	1.1e-75	Malic enzyme, N-terminal domain
Malic_M	PF03949	299 - 562	5.8e-89	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013057)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00570
fragaria_vesca	FvH4_7g13590
malus_domestica	MD01G1052400.v1.1 MD07G1129000.v1.1
prunus_persica	Prupe.2G160000_v2.0.a1 Prupe.2G160000_v2.0.a1 Prupe.2G160000_v2.0.a1
pyrus_communis	pycom01g07810 pycom07g12470
rosa_chinensis	RchiOBHm_Chr1g0353381
rosa_laevigata	RLG00000028273
rosa_multiflora	Rmu_sc0001207.1_g000013
rosa_roxburghii	Rroxscaffold_4G00301880
rosa_rugosa	Rorug01G0235600
rosa_samantha	Rh1AG247800 Rh1BG217600 Rh1CG231500
rosa_wichuraiana	Rw1G021590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	183, 533
AatII	GACGTC	1 cut(s)	448
Acc36I	ACCTGC	1 cut(s)	1330
AccB1I	GGYRCC	1 cut(s)	101
AccB7I	CCANNNNNTGG	1 cut(s)	1502
AccI	GTMKAC	4 cut(s)	47, 525, 955, 1207
AccII	CGCG	4 cut(s)	16, 99, 183, 1652
AccIII	TCCGGA	1 cut(s)	1638
AciI	CCGC	9 cut(s)	24, 55, 75, 99, 183, 1010, 1650, 1652, 1675
AclWI	GGATC	2 cut(s)	113, 317
AcoI	YGGCCR	1 cut(s)	509
AcsI	RAATTY	2 cut(s)	594, 770
AcuI	CTGAAG	1 cut(s)	1392
AcyI	GRCGYC	2 cut(s)	102, 445
AdeI	CACNNNGTG	1 cut(s)	200
AfaI	GTAC	6 cut(s)	347, 679, 752, 1038, 1287, 1619
AfiI	CCNNNNNNNGG	3 cut(s)	280, 407, 1502
AflIII	ACRYGT	4 cut(s)	791, 1237, 1482, 1740
AgeI	ACCGGT	1 cut(s)	658
AgsI	TTSAA	7 cut(s)	701, 830, 848, 886, 1214, 1276, 1314
AjnI	CCWGG	1 cut(s)	1495
AloI	GAACNNNNNNTCC	2 cut(s)	1005, 1037
Alw21I	GWGCWC	3 cut(s)	1516, 1531, 1734
Alw26I	GTCTC	3 cut(s)	191, 332, 1748
AlwI	GGATC	2 cut(s)	113, 317
AlwNI	CAGNNNCTG	1 cut(s)	1565
Aor13HI	TCCGGA	1 cut(s)	1638
AoxI	GGCC	8 cut(s)	271, 291, 509, 562, 797, 804, 1459, 1721
ApaI	GGGCCC	1 cut(s)	1725
ApoI	RAATTY	2 cut(s)	594, 770
AseI	ATTAAT	1 cut(s)	639
AsiGI	ACCGGT	1 cut(s)	658
Asp700I	GAANNNNTTC	1 cut(s)	783
AspA2I	CCTAGG	2 cut(s)	565, 731
AspLEI	GCGC	3 cut(s)	16, 99, 104
AspS9I	GGNCC	5 cut(s)	271, 571, 798, 1721, 1722
AsuC2I	CCSGG	2 cut(s)	1173, 1790
AsuHPI	GGTGA	2 cut(s)	1079, 1406
AvaII	GGWCC	1 cut(s)	571
AvrII	CCTAGG	2 cut(s)	565, 731
BaeGI	GKGCMC	1 cut(s)	1725
BalI	TGGCCA	1 cut(s)	511
BanI	GGYRCC	1 cut(s)	101
BanII	GRGCYC	3 cut(s)	1531, 1725, 1734
BauI	CACGAG	1 cut(s)	198
BbsI	GAAGAC	1 cut(s)	708
Bbv12I	GWGCWC	3 cut(s)	1516, 1531, 1734
BccI	CCATC	5 cut(s)	539, 1103, 1178, 1538, 1630
BceAI	ACGGC	1 cut(s)	81
BcgI	CGANNNNNNTGC	4 cut(s)	209, 243, 845, 879
BciT130I	CCWGG	1 cut(s)	1497
BciVI	GTATCC	2 cut(s)	566, 1646
BcnI	CCSGG	2 cut(s)	1173, 1790
BcoDI	GTCTC	3 cut(s)	191, 332, 1748
BfaI	CTAG	7 cut(s)	254, 566, 668, 732, 1115, 1203, 1805
BfmI	CTRYAG	2 cut(s)	402, 627
BfoI	RGCGCY	1 cut(s)	105
BfuAI	ACCTGC	1 cut(s)	1330
BfuI	GTATCC	2 cut(s)	566, 1646
BlnI	CCTAGG	2 cut(s)	565, 731
Bme1390I	CCNGG	3 cut(s)	1173, 1497, 1790
Bme18I	GGWCC	1 cut(s)	571
BmgT120I	GGNCC	5 cut(s)	271, 571, 798, 1721, 1722
BmiI	GGNNCC	5 cut(s)	103, 572, 1000, 1723, 1787
BmrFI	CCNGG	3 cut(s)	1173, 1497, 1790
BmsI	GCATC	3 cut(s)	1366, 1410, 1620
BpiI	GAAGAC	1 cut(s)	708
BplI	GAGNNNNNCTC	2 cut(s)	1503, 1535
BpmI	CTGGAG	1 cut(s)	1278
BpuMI	CCSGG	2 cut(s)	1173, 1790
Bsa29I	ATCGAT	1 cut(s)	169
BsaHI	GRCGYC	2 cut(s)	102, 445
BsaI	GGTCTC	2 cut(s)	191, 332
BsaWI	WCCGGW	2 cut(s)	658, 1638
BsaXI	ACNNNNNCTCC	4 cut(s)	476, 506, 1653, 1683
Bsc4I	CCNNNNNNNGG	3 cut(s)	280, 407, 1502
Bse118I	RCCGGY	3 cut(s)	42, 658, 908
Bse1I	ACTGG	3 cut(s)	148, 512, 1701
Bse3DI	GCAATG	1 cut(s)	1302
BseAI	TCCGGA	1 cut(s)	1638
BseBI	CCWGG	1 cut(s)	1497
BseCI	ATCGAT	1 cut(s)	169
BseGI	GGATG	1 cut(s)	369
BseLI	CCNNNNNNNGG	3 cut(s)	280, 407, 1502
BseMI	GCAATG	1 cut(s)	1302
BseMII	CTCAG	1 cut(s)	1536
BseNI	ACTGG	3 cut(s)	148, 512, 1701
BseRI	GAGGAG	2 cut(s)	179, 1766
BseSI	GKGCMC	1 cut(s)	1725
BsgI	GTGCAG	2 cut(s)	833, 1137
Bsh1236I	CGCG	4 cut(s)	16, 99, 183, 1652
Bsh1285I	CGRYCG	1 cut(s)	47
BshFI	GGCC	8 cut(s)	273, 293, 511, 564, 799, 806, 1461, 1723
BshNI	GGYRCC	1 cut(s)	101
BshTI	ACCGGT	1 cut(s)	658
BshVI	ATCGAT	1 cut(s)	169
BsiEI	CGRYCG	1 cut(s)	47
BsiHKAI	GWGCWC	3 cut(s)	1516, 1531, 1734
BsiSI	CCGG	7 cut(s)	43, 659, 909, 1002, 1172, 1639, 1790
BslFI	GGGAC	4 cut(s)	432, 584, 1026, 1719
BslI	CCNNNNNNNGG	3 cut(s)	280, 407, 1502
BsmAI	GTCTC	3 cut(s)	191, 332, 1748
BsmFI	GGGAC	4 cut(s)	432, 584, 1026, 1719
BsmI	GAATGC	3 cut(s)	1360, 1367, 1574
BsnI	GGCC	8 cut(s)	273, 293, 511, 564, 799, 806, 1461, 1723
Bso31I	GGTCTC	2 cut(s)	191, 332
Bsp120I	GGGCCC	1 cut(s)	1721
Bsp1286I	GDGCHC	4 cut(s)	1516, 1531, 1725, 1734
Bsp13I	TCCGGA	1 cut(s)	1638
Bsp1407I	TGTACA	1 cut(s)	1036
Bsp143I	GATC	4 cut(s)	118, 166, 309, 498
Bsp19I	CCATGG	2 cut(s)	122, 1146
BspACI	CCGC	9 cut(s)	24, 55, 75, 99, 183, 1010, 1650, 1652, 1675
BspANI	GGCC	8 cut(s)	273, 293, 511, 564, 799, 806, 1461, 1723
BspCNI	CTCAG	1 cut(s)	1535
BspDI	ATCGAT	1 cut(s)	169
BspEI	TCCGGA	1 cut(s)	1638
BspFNI	CGCG	4 cut(s)	16, 99, 183, 1652
BspHI	TCATGA	2 cut(s)	1279, 1734
BspLI	GGNNCC	5 cut(s)	103, 572, 1000, 1723, 1787
BspMAI	CTGCAG	1 cut(s)	631
BspMI	ACCTGC	1 cut(s)	1330
BspPI	GGATC	2 cut(s)	113, 317
BspT107I	GGYRCC	1 cut(s)	101
BspTNI	GGTCTC	2 cut(s)	191, 332
BsrDI	GCAATG	1 cut(s)	1302
BsrFI	RCCGGY	3 cut(s)	42, 658, 908
BsrGI	TGTACA	1 cut(s)	1036
BsrI	ACTGG	3 cut(s)	148, 512, 1701
BssAI	RCCGGY	3 cut(s)	42, 658, 908
BssMI	GATC	4 cut(s)	118, 166, 309, 498
BssNI	GRCGYC	2 cut(s)	102, 445
BssSI	CACGAG	1 cut(s)	198
BssT1I	CCWWGG	9 cut(s)	122, 294, 559, 565, 574, 731, 801, 1146, 1441
Bst2BI	CACGAG	1 cut(s)	198
Bst2UI	CCWGG	1 cut(s)	1497
Bst4CI	ACNGT	7 cut(s)	353, 406, 707, 940, 1067, 1712, 1759
Bst6I	CTCTTC	1 cut(s)	741
BstACI	GRCGYC	2 cut(s)	102, 445
BstAPI	GCANNNNNTGC	2 cut(s)	1160, 1685
BstAUI	TGTACA	1 cut(s)	1036
BstC8I	GCNNGC	4 cut(s)	275, 513, 1389, 1677
BstDEI	CTNAG	3 cut(s)	267, 1190, 1522
BstDSI	CCRYGG	4 cut(s)	122, 451, 1146, 1650
BstENI	CCTNNNNNAGG	1 cut(s)	405
BstF5I	GGATG	1 cut(s)	369
BstFNI	CGCG	4 cut(s)	16, 99, 183, 1652
BstH2I	RGCGCY	1 cut(s)	105
BstHHI	GCGC	3 cut(s)	16, 99, 104
BstKTI	GATC	4 cut(s)	121, 169, 312, 501
BstMAI	GTCTC	3 cut(s)	191, 332, 1748
BstMBI	GATC	4 cut(s)	118, 166, 309, 498
BstMCI	CGRYCG	1 cut(s)	47
BstNI	CCWGG	1 cut(s)	1497
BstNSI	RCATGY	5 cut(s)	1241, 1391, 1486, 1552, 1744
BstSCI	CCNGG	3 cut(s)	1171, 1495, 1788
BstSFI	CTRYAG	2 cut(s)	402, 627
BstSLI	GKGCMC	1 cut(s)	1725
BstUI	CGCG	4 cut(s)	16, 99, 183, 1652
BstV2I	GAAGAC	1 cut(s)	708
BstX2I	RGATCY	1 cut(s)	309
BstYI	RGATCY	1 cut(s)	309
Bsu15I	ATCGAT	1 cut(s)	169
BsuI	GTATCC	2 cut(s)	566, 1646
BsuRI	GGCC	8 cut(s)	273, 293, 511, 564, 799, 806, 1461, 1723
BsuTUI	ATCGAT	1 cut(s)	169
BtgI	CCRYGG	4 cut(s)	122, 451, 1146, 1650
BtsCI	GGATG	1 cut(s)	369
BtsI	GCAGTG	2 cut(s)	851, 1365
BtsIMutI	CAGTG	5 cut(s)	141, 472, 851, 1365, 1708
BveI	ACCTGC	1 cut(s)	1330
Cac8I	GCNNGC	4 cut(s)	275, 513, 1389, 1677
CaiI	CAGNNNCTG	1 cut(s)	1565
CciI	TCATGA	2 cut(s)	1279, 1734
CfoI	GCGC	3 cut(s)	16, 99, 104
Cfr10I	RCCGGY	3 cut(s)	42, 658, 908
Cfr13I	GGNCC	5 cut(s)	271, 571, 798, 1721, 1722
Cfr42I	CCGCGG	1 cut(s)	1653
ClaI	ATCGAT	1 cut(s)	169
Csp6I	GTAC	6 cut(s)	346, 678, 751, 1037, 1286, 1618
CspAI	ACCGGT	1 cut(s)	658
CspCI	CAANNNNNGTGG	2 cut(s)	818, 853
CviQI	GTAC	6 cut(s)	346, 678, 751, 1037, 1286, 1618
DdeI	CTNAG	3 cut(s)	267, 1190, 1522
DinI	GGCGCC	1 cut(s)	103
DpnI	GATC	4 cut(s)	120, 168, 311, 500
DpnII	GATC	4 cut(s)	118, 166, 309, 498
DraIII	CACNNNGTG	1 cut(s)	200
DrdI	GACNNNNNNGTC	2 cut(s)	183, 533
DseDI	GACNNNNNNGTC	2 cut(s)	183, 533
EaeI	YGGCCR	1 cut(s)	509
Eam1104I	CTCTTC	1 cut(s)	741
EarI	CTCTTC	1 cut(s)	741
Ecl136II	GAGCTC	2 cut(s)	1529, 1732
Eco130I	CCWWGG	9 cut(s)	122, 294, 559, 565, 574, 731, 801, 1146, 1441
Eco24I	GRGCYC	3 cut(s)	1531, 1725, 1734
Eco31I	GGTCTC	2 cut(s)	191, 332
Eco32I	GATATC	1 cut(s)	1600
Eco47I	GGWCC	1 cut(s)	571
Eco53kI	GAGCTC	2 cut(s)	1529, 1732
Eco57I	CTGAAG	1 cut(s)	1392
EcoICRI	GAGCTC	2 cut(s)	1529, 1732
EcoNI	CCTNNNNNAGG	1 cut(s)	405
EcoO109I	RGGNCCY	1 cut(s)	571
EcoRI	GAATTC	2 cut(s)	594, 770
EcoRII	CCWGG	1 cut(s)	1495
EcoRV	GATATC	1 cut(s)	1600
EcoT14I	CCWWGG	9 cut(s)	122, 294, 559, 565, 574, 731, 801, 1146, 1441
EcoT38I	GRGCYC	3 cut(s)	1531, 1725, 1734
EgeI	GGCGCC	1 cut(s)	103
EheI	GGCGCC	1 cut(s)	103
ErhI	CCWWGG	9 cut(s)	122, 294, 559, 565, 574, 731, 801, 1146, 1441
FalI	AAGNNNNNCTT	4 cut(s)	693, 725, 1598, 1630
FaqI	GGGAC	4 cut(s)	432, 584, 1026, 1719
FauI	CCCGC	3 cut(s)	82, 1003, 1668
FblI	GTMKAC	4 cut(s)	47, 525, 955, 1207
FokI	GGATG	1 cut(s)	356
FriOI	GRGCYC	3 cut(s)	1531, 1725, 1734
FspBI	CTAG	7 cut(s)	254, 566, 668, 732, 1115, 1203, 1805
GlaI	GCGC	3 cut(s)	15, 98, 103
GsuI	CTGGAG	1 cut(s)	1278
HaeII	RGCGCY	1 cut(s)	105
HaeIII	GGCC	8 cut(s)	273, 293, 511, 564, 799, 806, 1461, 1723
HapII	CCGG	7 cut(s)	43, 659, 909, 1002, 1172, 1639, 1790
HhaI	GCGC	3 cut(s)	16, 99, 104
Hin1I	GRCGYC	2 cut(s)	102, 445
Hin6I	GCGC	3 cut(s)	14, 97, 102
HinP1I	GCGC	3 cut(s)	14, 97, 102
HincII	GTYRAC	4 cut(s)	48, 156, 956, 1438
HindII	GTYRAC	4 cut(s)	48, 156, 956, 1438
HinfI	GANTC	6 cut(s)	18, 70, 239, 1304, 1310, 1771
HpaII	CCGG	7 cut(s)	43, 659, 909, 1002, 1172, 1639, 1790
HphI	GGTGA	2 cut(s)	1079, 1406
Hpy166II	GTNNAC	9 cut(s)	48, 91, 156, 526, 956, 1208, 1438, 1795, 1810
Hpy188I	TCNGA	3 cut(s)	1309, 1543, 1567
Hpy188III	TCNNGA	9 cut(s)	61, 1139, 1257, 1280, 1524, 1603, 1639, 1735, 1811
Hpy8I	GTNNAC	9 cut(s)	48, 91, 156, 526, 956, 1208, 1438, 1795, 1810
Hpy99I	CGWCG	2 cut(s)	52, 67
HpyAV	CCTTC	3 cut(s)	734, 1219, 1600
HpyCH4III	ACNGT	7 cut(s)	353, 406, 707, 940, 1067, 1712, 1759
HpyCH4IV	ACGT	3 cut(s)	445, 793, 881
HpyF3I	CTNAG	3 cut(s)	267, 1190, 1522
HpySE526I	ACGT	3 cut(s)	445, 793, 881
Hsp92I	GRCGYC	2 cut(s)	102, 445
HspAI	GCGC	3 cut(s)	14, 97, 102
KasI	GGCGCC	1 cut(s)	101
Kpn2I	TCCGGA	1 cut(s)	1638
KspI	CCGCGG	1 cut(s)	1653
Kzo9I	GATC	4 cut(s)	118, 166, 309, 498
LmnI	GCTCC	7 cut(s)	388, 998, 1175, 1199, 1511, 1526, 1661
LweI	GCATC	3 cut(s)	1366, 1410, 1620
MaeI	CTAG	7 cut(s)	254, 566, 668, 732, 1115, 1203, 1805
MaeII	ACGT	3 cut(s)	445, 793, 881
MaeIII	GTNAC	3 cut(s)	965, 1117, 1551
MalI	GATC	4 cut(s)	120, 168, 311, 500
MboI	GATC	4 cut(s)	118, 166, 309, 498
MboII	GAAGA	4 cut(s)	713, 758, 1385, 1703
MfeI	CAATTG	3 cut(s)	600, 759, 1179
MflI	RGATCY	1 cut(s)	309
MhlI	GDGCHC	4 cut(s)	1516, 1531, 1725, 1734
MlsI	TGGCCA	1 cut(s)	511
MluNI	TGGCCA	1 cut(s)	511
Mly113I	GGCGCC	1 cut(s)	102
MlyI	GAGTC	2 cut(s)	248, 1780
MmeI	TCCRAC	3 cut(s)	717, 1370, 1639
Mox20I	TGGCCA	1 cut(s)	511
MroI	TCCGGA	1 cut(s)	1638
MroXI	GAANNNNTTC	1 cut(s)	783
MscI	TGGCCA	1 cut(s)	511
MseI	TTAA	7 cut(s)	129, 314, 639, 1026, 1221, 1290, 1383
MslI	CAYNNNNRTG	2 cut(s)	1392, 1739
Msp20I	TGGCCA	1 cut(s)	511
MspA1I	CMGCKG	3 cut(s)	632, 1652, 1682
MspI	CCGG	7 cut(s)	43, 659, 909, 1002, 1172, 1639, 1790
MspR9I	CCNGG	3 cut(s)	1173, 1497, 1790
MteI	GCGCNGCGC	1 cut(s)	100
MunI	CAATTG	3 cut(s)	600, 759, 1179
Mva1269I	GAATGC	3 cut(s)	1360, 1367, 1574
MvaI	CCWGG	1 cut(s)	1497
MvnI	CGCG	4 cut(s)	16, 99, 183, 1652
NarI	GGCGCC	1 cut(s)	102
NciI	CCSGG	2 cut(s)	1173, 1790
NcoI	CCATGG	2 cut(s)	122, 1146
NdeII	GATC	4 cut(s)	118, 166, 309, 498
NlaIV	GGNNCC	5 cut(s)	103, 572, 1000, 1723, 1787
NmuCI	GTSAC	1 cut(s)	965
NspI	RCATGY	5 cut(s)	1241, 1391, 1486, 1552, 1744
PaeI	GCATGC	1 cut(s)	1391
PagI	TCATGA	2 cut(s)	1279, 1734
PciI	ACATGT	3 cut(s)	1237, 1482, 1740
PctI	GAATGC	3 cut(s)	1360, 1367, 1574
PdmI	GAANNNNTTC	1 cut(s)	783
PfeI	GAWTC	4 cut(s)	18, 70, 1304, 1310
PflMI	CCANNNNNTGG	1 cut(s)	1502
PinAI	ACCGGT	1 cut(s)	658
PleI	GAGTC	2 cut(s)	247, 1779
PluTI	GGCGCC	1 cut(s)	105
PpsI	GAGTC	2 cut(s)	247, 1779
PpuMI	RGGWCCY	1 cut(s)	571
PscI	ACATGT	3 cut(s)	1237, 1482, 1740
PshBI	ATTAAT	1 cut(s)	639
Psp124BI	GAGCTC	2 cut(s)	1531, 1734
Psp5II	RGGWCCY	1 cut(s)	571
Psp6I	CCWGG	1 cut(s)	1495
PspGI	CCWGG	1 cut(s)	1495
PspN4I	GGNNCC	5 cut(s)	103, 572, 1000, 1723, 1787
PspOMI	GGGCCC	1 cut(s)	1721
PspPI	GGNCC	5 cut(s)	271, 571, 798, 1721, 1722
PspPPI	RGGWCCY	1 cut(s)	571
PstI	CTGCAG	1 cut(s)	631
PstNI	CAGNNNCTG	1 cut(s)	1565
PsuI	RGATCY	1 cut(s)	309
PvuII	CAGCTG	2 cut(s)	632, 1682
RsaI	GTAC	6 cut(s)	347, 679, 752, 1038, 1287, 1619
RsaNI	GTAC	6 cut(s)	346, 678, 751, 1037, 1286, 1618
RseI	CAYNNNNRTG	2 cut(s)	1392, 1739
SacI	GAGCTC	2 cut(s)	1531, 1734
SacII	CCGCGG	1 cut(s)	1653
SalI	GTCGAC	2 cut(s)	46, 954
SaqAI	TTAA	7 cut(s)	129, 314, 639, 1026, 1221, 1290, 1383
Sau3AI	GATC	4 cut(s)	118, 166, 309, 498
Sau96I	GGNCC	5 cut(s)	271, 571, 798, 1721, 1722
SchI	GAGTC	2 cut(s)	248, 1780
ScrFI	CCNGG	3 cut(s)	1173, 1497, 1790
SduI	GDGCHC	4 cut(s)	1516, 1531, 1725, 1734
SfaNI	GCATC	3 cut(s)	1366, 1410, 1620
SfcI	CTRYAG	2 cut(s)	402, 627
SfoI	GGCGCC	1 cut(s)	103
Sfr303I	CCGCGG	1 cut(s)	1653
SgrBI	CCGCGG	1 cut(s)	1653
SinI	GGWCC	1 cut(s)	571
SmiMI	CAYNNNNRTG	2 cut(s)	1392, 1739
SphI	GCATGC	1 cut(s)	1391
SsiI	CCGC	9 cut(s)	24, 55, 75, 99, 183, 1010, 1650, 1652, 1675
SspDI	GGCGCC	1 cut(s)	101
SspMI	CTAG	7 cut(s)	254, 566, 668, 732, 1115, 1203, 1805
SstI	GAGCTC	2 cut(s)	1531, 1734
StyD4I	CCNGG	3 cut(s)	1171, 1495, 1788
StyI	CCWWGG	9 cut(s)	122, 294, 559, 565, 574, 731, 801, 1146, 1441
TaaI	ACNGT	7 cut(s)	353, 406, 707, 940, 1067, 1712, 1759
TaiI	ACGT	3 cut(s)	448, 796, 884
TaqI	TCGA	6 cut(s)	47, 62, 169, 213, 724, 955
TatI	WGTACW	3 cut(s)	345, 750, 1036
TauI	GCSGC	2 cut(s)	26, 102
TfiI	GAWTC	4 cut(s)	18, 70, 1304, 1310
Tru1I	TTAA	7 cut(s)	129, 314, 639, 1026, 1221, 1290, 1383
Tru9I	TTAA	7 cut(s)	129, 314, 639, 1026, 1221, 1290, 1383
TscAI	CASTG	5 cut(s)	148, 472, 858, 1372, 1715
TseFI	GTSAC	1 cut(s)	965
Tsp45I	GTSAC	1 cut(s)	965
TspDTI	ATGAA	8 cut(s)	223, 763, 776, 783, 848, 1367, 1569, 1751
TspGWI	ACGGA	2 cut(s)	141, 1464
TspRI	CASTG	5 cut(s)	148, 472, 858, 1372, 1715
Van91I	CCANNNNNTGG	1 cut(s)	1502
VpaK11BI	GGWCC	1 cut(s)	571
VspI	ATTAAT	1 cut(s)	639
XagI	CCTNNNNNAGG	1 cut(s)	405
XapI	RAATTY	2 cut(s)	594, 770
XceI	RCATGY	5 cut(s)	1241, 1391, 1486, 1552, 1744
XmaJI	CCTAGG	2 cut(s)	565, 731
XmiI	GTMKAC	4 cut(s)	47, 525, 955, 1207
XmnI	GAANNNNTTC	1 cut(s)	783
XspI	CTAG	7 cut(s)	254, 566, 668, 732, 1115, 1203, 1805
ZraI	GACGTC	1 cut(s)	446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.