AT4G02080
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017489
Physical Location & Seq
Forward (+)
921447 .. 922759
1313 bp
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UTR
Exon/CDS
Intron
AT4G02080.1

Sequence Viewer

Length: 582 bp
ATGTTCATGATCGATTGGTTCTATGGCGTTCTCGCTTCGTTAGGGTTATGGCAGAAAGAGGCTAAGATCTTGTTTCTGGGACTCGATAATGCTGGTAAAACCACTCTGCTTCACATGTTGAAAGACGAGAGATTGGTACAGCATCAGCCTACGCAGCATCCAACTTCTGAAGAACTCAGCATTGGGAAAATCAAGTTTAAGGCTTTTGATTTGGGTGGTCACCAGATTGCTCGCAGGGTCTGGAAGGATTACTATGCTAAGGTGGACGCTGTGGTCTACCTAGTTGATGCTTACGACAAAGAGAGATTTGCAGAATCAAAAAAGGAACTTGATGCACTTCTCTCAGACGAATCCCTAGCCAGCGTTCCATTCCTCATTCTAGGAAACAAGATAGACATACCGTATGCTGCATCAGAGGACGAGCTCCGTTACCATCTCGGCCTCTCCAACTTCACTACAGGAAAGGGTAAAGTGAATCTAACGGATTCGAACGTTAGGCCATTGGAGGTTTTCATGTGCAGCATTGTCAGGAAAATGGGTTACGGAGAAGGTTTCAAATGGGTTTCTCAATACATCAAGTAA

Protein Analysis

193

Amino Acids

22.03

Weight (kDa)

6.97

Isoelectric Point (pI)

25.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 6e-65 ADP-ribosylation factor family
SRPRB PF09439 19 - 144 3.8e-08 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 1.9e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 1e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 7.8e-07 Ras family
G-alpha PF00503 49 - 133 4.1e-09 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0017489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02080
fragaria_vesca FvH4_4g19410
malus_domestica MD16G1157800.v1.1
pyrus_communis pycom13g13440
rosa_chinensis RchiOBHm_Chr4g0424441
rosa_laevigata RLG00000007474
rosa_multiflora Rmu_sc0003567.1_g000010
rosa_roxburghii Rroxscaffold_5G00366380
rosa_samantha Rh4AG253900 Rh4BG259200 Rh4CG270500 Rh4DG253700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
AccI GTMKAC 1 cut(s) 276
AclI AACGTT 1 cut(s) 492
AcuI CTGAAG 1 cut(s) 189
AfaI GTAC 1 cut(s) 138
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 2 cut(s) 121, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 165, 197
AluBI AGCT 1 cut(s) 424
AluI AGCT 1 cut(s) 424
Alw21I GWGCWC 1 cut(s) 426
AlwNI CAGNNNCTG 1 cut(s) 240
AoxI GGCC 2 cut(s) 439, 497
ApeKI GCWGC 3 cut(s) 154, 407, 519
AsuHPI GGTGA 1 cut(s) 212
AsuII TTCGAA 1 cut(s) 488
BanII GRGCYC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 3 cut(s) 166, 394, 531
BccI CCATC 1 cut(s) 441
BfaI CTAG 3 cut(s) 281, 356, 380
BfmI CTRYAG 1 cut(s) 456
BglII AGATCT 1 cut(s) 66
BisI GCNGC 3 cut(s) 155, 408, 520
BlsI GCNGC 3 cut(s) 156, 409, 521
BmsI GCATC 5 cut(s) 151, 166, 277, 322, 419
Bpu10I CCTNAGC 1 cut(s) 258
Bpu14I TTCGAA 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 12
BseCI ATCGAT 1 cut(s) 12
BseGI GGATG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 190, 357
BseXI GCAGC 3 cut(s) 166, 394, 531
BsgI GTGCAG 1 cut(s) 538
BshFI GGCC 2 cut(s) 441, 499
BshVI ATCGAT 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 426
BslFI GGGAC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 93
BsnI GGCC 2 cut(s) 441, 499
Bsp119I TTCGAA 1 cut(s) 488
Bsp1286I GDGCHC 1 cut(s) 426
Bsp143I GATC 2 cut(s) 9, 66
BspANI GGCC 2 cut(s) 441, 499
BspCNI CTCAG 2 cut(s) 189, 356
BspDI ATCGAT 1 cut(s) 12
BspHI TCATGA 1 cut(s) 6
BspT104I TTCGAA 1 cut(s) 488
BssMI GATC 2 cut(s) 9, 66
Bst4CI ACNGT 1 cut(s) 402
BstBI TTCGAA 1 cut(s) 488
BstC8I GCNNGC 2 cut(s) 232, 361
BstDEI CTNAG 4 cut(s) 63, 176, 258, 343
BstEII GGTNACC 1 cut(s) 218
BstF5I GGATG 1 cut(s) 157
BstKTI GATC 2 cut(s) 12, 69
BstMBI GATC 2 cut(s) 9, 66
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstNSI RCATGY 1 cut(s) 118
BstPI GGTNACC 1 cut(s) 218
BstSFI CTRYAG 1 cut(s) 456
BstV1I GCAGC 3 cut(s) 166, 394, 531
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
Bsu15I ATCGAT 1 cut(s) 12
BsuRI GGCC 2 cut(s) 441, 499
BsuTUI ATCGAT 1 cut(s) 12
BtsCI GGATG 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 232, 361
CaiI CAGNNNCTG 1 cut(s) 240
CciI TCATGA 1 cut(s) 6
ClaI ATCGAT 1 cut(s) 12
CseI GACGC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 137
CviAII CATG 3 cut(s) 7, 115, 514
CviJI RGCY 7 cut(s) 62, 148, 203, 359, 424, 441, 499
CviKI_1 RGCY 7 cut(s) 62, 148, 203, 359, 424, 441, 499
CviQI GTAC 1 cut(s) 137
DdeI CTNAG 4 cut(s) 63, 176, 258, 343
DpnI GATC 2 cut(s) 11, 68
DpnII GATC 2 cut(s) 9, 66
DrdI GACNNNNNNGTC 1 cut(s) 272
DseDI GACNNNNNNGTC 1 cut(s) 272
Ecl136II GAGCTC 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 426
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 189
Eco91I GGTNACC 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 218
EcoT38I GRGCYC 1 cut(s) 426
FaeI CATG 3 cut(s) 10, 118, 517
FaiI YATR 8 cut(s) 8, 24, 49, 116, 255, 398, 405, 515
FaqI GGGAC 1 cut(s) 93
FatI CATG 3 cut(s) 6, 114, 513
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 3 cut(s) 155, 408, 520
FokI GGATG 1 cut(s) 144
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 3 cut(s) 155, 408, 520
FspBI CTAG 3 cut(s) 281, 356, 380
GluI GCNGC 3 cut(s) 155, 408, 520
HaeIII GGCC 2 cut(s) 441, 499
HgaI GACGC 1 cut(s) 275
Hin1II CATG 3 cut(s) 10, 118, 517
HinfI GANTC 5 cut(s) 81, 314, 350, 475, 485
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 2 cut(s) 265, 277
Hpy188I TCNGA 3 cut(s) 169, 346, 415
Hpy188III TCNNGA 3 cut(s) 7, 241, 529
Hpy8I GTNNAC 2 cut(s) 265, 277
HpyAV CCTTC 2 cut(s) 238, 542
HpyCH4III ACNGT 1 cut(s) 402
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 4 cut(s) 311, 335, 410, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
HpyF3I CTNAG 4 cut(s) 63, 176, 258, 343
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 3 cut(s) 10, 118, 517
Kzo9I GATC 2 cut(s) 9, 66
LmnI GCTCC 1 cut(s) 429
LpnPI CCDG 8 cut(s) 62, 78, 220, 226, 236, 373, 444, 514
Lsp1109I GCAGC 3 cut(s) 166, 394, 531
LweI GCATC 5 cut(s) 151, 166, 277, 322, 419
MaeI CTAG 3 cut(s) 281, 356, 380
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 3 cut(s) 218, 428, 539
MalI GATC 2 cut(s) 11, 68
MboI GATC 2 cut(s) 9, 66
MboII GAAGA 1 cut(s) 182
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 426
MlyI GAGTC 1 cut(s) 75
MmeI TCCRAC 2 cut(s) 185, 471
MnlI CCTC 5 cut(s) 52, 383, 409, 452, 499
MseI TTAA 1 cut(s) 198
MwoI GCNNNNNNNGC 1 cut(s) 154
NdeII GATC 2 cut(s) 9, 66
NlaIII CATG 3 cut(s) 10, 118, 517
NmeAIII GCCGAG 1 cut(s) 417
NmuCI GTSAC 1 cut(s) 218
NspI RCATGY 1 cut(s) 118
NspV TTCGAA 1 cut(s) 488
PagI TCATGA 1 cut(s) 6
PciI ACATGT 1 cut(s) 114
PfeI GAWTC 4 cut(s) 314, 350, 475, 485
PkrI GCNGC 3 cut(s) 156, 409, 521
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PscI ACATGT 1 cut(s) 114
Psp124BI GAGCTC 1 cut(s) 426
Psp1406I AACGTT 1 cut(s) 492
PspEI GGTNACC 1 cut(s) 218
PstNI CAGNNNCTG 1 cut(s) 240
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 138
RsaNI GTAC 1 cut(s) 137
SacI GAGCTC 1 cut(s) 426
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 3 cut(s) 155, 408, 520
Sau3AI GATC 2 cut(s) 9, 66
SchI GAGTC 1 cut(s) 75
SduI GDGCHC 1 cut(s) 426
SetI ASST 6 cut(s) 264, 282, 426, 495, 510, 553
SfaNI GCATC 5 cut(s) 151, 166, 277, 322, 419
SfcI CTRYAG 1 cut(s) 456
SfuI TTCGAA 1 cut(s) 488
SspMI CTAG 3 cut(s) 281, 356, 380
SstI GAGCTC 1 cut(s) 426
TaaI ACNGT 1 cut(s) 402
TaiI ACGT 1 cut(s) 495
TaqI TCGA 3 cut(s) 12, 84, 488
TfiI GAWTC 4 cut(s) 314, 350, 475, 485
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 3 cut(s) 154, 407, 519
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 1 cut(s) 502
TspGWI ACGGA 3 cut(s) 416, 497, 558
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 3 cut(s) 281, 356, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.