RchiOBHm_Chr4g0424441
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017489
Physical Location & Seq
Reverse (-)
50056722 .. 50057687
966 bp
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UTR
Exon/CDS
Intron
PRQ39371

Sequence Viewer

Length: 678 bp
ATGTTTCTTGTGGATTGGTTCTATGGAATTCTTGAATCGTTGGGGCTATGGCATAAAGACGCTAGGATCTGTTTCTTAGGTCTTGATAATGCTGGAAAGACCACTTTGCTTCACATGCTCAGATTTGGGGTACGTACTTCTTCTCTCTATCTTCATCAATCTCTAGTTTTCTGGGTCTCTTCTTTTGTTCTCTCTATTTTCATATCGATCATGTTCCACTTGCAGAGCTTGAAGCAAAGCGAACCAACTCAGCAACCAACCTCGGATGATTTTTCAATAGGGAGTGTCCACTTCACAGCCTTTGATTTGGGTGGGCATCAGACCGCTCGCAGAGTCTGGAAGGATTACTATGCCCAGCTGGATGCTGTAGTCTACGTAATTGATGCTCATGACAAGGAAAGATTTGCAGAGTCGAGAAGGGAGCTCGATTCTCTTCTCTCTGAAGAGTCTCTGGCAACAGTGCCGTTTCTTATTTTGGGAAACAAGATCGACTTGCCTTACTCTGCCTCAGAGGAGGAGCTGCGTGCTTCTCTCGGCCTGACCAACTTCACAACTGGCAAGGGTAAGGTCAAGATGTACCATTCCAATGTCCGCCCCATGGAGGTGTTCATGTGCAGCATTGCTCGCAAGATGGGTTATGGGGAAGGATTCAAGTGGCTTGCTCAGTATATCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

225

Amino Acids

25.76

Weight (kDa)

6.24

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 43 2.2e-08 ADP-ribosylation factor family
GTP_EFTU PF00009 23 - 176 6.6e-06 Elongation factor Tu GTP binding domain
Arf PF00025 71 - 224 8.8e-44 ADP-ribosylation factor family
G-alpha PF00503 81 - 165 1.5e-07 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02080
fragaria_vesca FvH4_4g19410
malus_domestica MD16G1157800.v1.1
pyrus_communis pycom13g13440
rosa_chinensis RchiOBHm_Chr4g0424441
rosa_laevigata RLG00000007474
rosa_multiflora Rmu_sc0003567.1_g000010
rosa_roxburghii Rroxscaffold_5G00366380
rosa_samantha Rh4AG253900 Rh4BG259200 Rh4CG270500 Rh4DG253700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 372
AciI CCGC 2 cut(s) 324, 592
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 1 cut(s) 27
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 3 cut(s) 132, 136, 578
AfiI CCNNNNNNNGG 2 cut(s) 598, 601
AgsI TTSAA 4 cut(s) 35, 232, 276, 652
AluBI AGCT 4 cut(s) 228, 358, 424, 520
AluI AGCT 4 cut(s) 228, 358, 424, 520
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 2 cut(s) 181, 453
AlwI GGATC 1 cut(s) 74
AlwNI CAGNNNCTG 1 cut(s) 336
AoxI GGCC 1 cut(s) 535
ApeKI GCWGC 2 cut(s) 520, 615
ApoI RAATTY 1 cut(s) 27
BanII GRGCYC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 2 cut(s) 507, 627
BccI CCATC 1 cut(s) 625
BceAI ACGGC 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 181, 453
BfaI CTAG 2 cut(s) 63, 164
BfmI CTRYAG 1 cut(s) 366
BisI GCNGC 2 cut(s) 521, 616
BlsI GCNGC 2 cut(s) 522, 617
BmsI GCATC 3 cut(s) 325, 352, 373
Bsa29I ATCGAT 1 cut(s) 206
BsaAI YACGTR 2 cut(s) 134, 376
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 2 cut(s) 261, 597
Bsc4I CCNNNNNNNGG 2 cut(s) 598, 601
Bse1I ACTGG 1 cut(s) 559
Bse3DI GCAATG 1 cut(s) 618
BseCI ATCGAT 1 cut(s) 206
BseDI CCNNGG 2 cut(s) 261, 597
BseGI GGATG 2 cut(s) 271, 367
BseLI CCNNNNNNNGG 2 cut(s) 598, 601
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 4 cut(s) 133, 263, 522, 677
BseNI ACTGG 1 cut(s) 559
BseRI GAGGAG 2 cut(s) 527, 530
BseXI GCAGC 2 cut(s) 507, 627
BseYI CCCAGC 1 cut(s) 354
BsgI GTGCAG 1 cut(s) 634
BshFI GGCC 1 cut(s) 537
BshVI ATCGAT 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 426
BslI CCNNNNNNNGG 2 cut(s) 598, 601
BsmAI GTCTC 2 cut(s) 181, 453
BsnI GGCC 1 cut(s) 537
Bso31I GGTCTC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 426
Bsp143I GATC 3 cut(s) 66, 207, 486
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 2 cut(s) 324, 592
BspANI GGCC 1 cut(s) 537
BspCNI CTCAG 4 cut(s) 132, 262, 521, 676
BspDI ATCGAT 1 cut(s) 206
BspHI TCATGA 1 cut(s) 388
BspPI GGATC 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 181
BsrBI CCGCTC 1 cut(s) 326
BsrDI GCAATG 1 cut(s) 618
BsrI ACTGG 1 cut(s) 559
BssECI CCNNGG 2 cut(s) 261, 597
BssMI GATC 3 cut(s) 66, 207, 486
BssT1I CCWWGG 1 cut(s) 597
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 3 cut(s) 184, 438, 438
BstBAI YACGTR 2 cut(s) 134, 376
BstC8I GCNNGC 4 cut(s) 328, 525, 625, 660
BstDEI CTNAG 5 cut(s) 76, 119, 249, 508, 663
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 2 cut(s) 271, 367
BstKTI GATC 3 cut(s) 69, 210, 489
BstMAI GTCTC 2 cut(s) 181, 453
BstMBI GATC 3 cut(s) 66, 207, 486
BstMWI GCNNNNNNNGC 2 cut(s) 115, 624
BstNSI RCATGY 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 366
BstSNI TACGTA 2 cut(s) 134, 376
BstV1I GCAGC 2 cut(s) 507, 627
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
Bsu15I ATCGAT 1 cut(s) 206
BsuRI GGCC 1 cut(s) 537
BsuTUI ATCGAT 1 cut(s) 206
BtgI CCRYGG 1 cut(s) 597
BtsCI GGATG 2 cut(s) 271, 367
BtsIMutI CAGTG 1 cut(s) 465
Cac8I GCNNGC 4 cut(s) 328, 525, 625, 660
CaiI CAGNNNCTG 1 cut(s) 336
CciI TCATGA 1 cut(s) 388
ClaI ATCGAT 1 cut(s) 206
CseI GACGC 1 cut(s) 68
Csp6I GTAC 3 cut(s) 131, 135, 577
CviAII CATG 5 cut(s) 115, 211, 389, 598, 610
CviJI RGCY 8 cut(s) 46, 228, 299, 358, 424, 520, 537, 658
CviKI_1 RGCY 8 cut(s) 46, 228, 299, 358, 424, 520, 537, 658
CviQI GTAC 3 cut(s) 131, 135, 577
DdeI CTNAG 5 cut(s) 76, 119, 249, 508, 663
DpnI GATC 3 cut(s) 68, 209, 488
DpnII GATC 3 cut(s) 66, 207, 486
Eam1104I CTCTTC 3 cut(s) 184, 438, 438
EarI CTCTTC 3 cut(s) 184, 438, 438
EciI GGCGGA 1 cut(s) 581
Ecl136II GAGCTC 1 cut(s) 424
Eco105I TACGTA 2 cut(s) 134, 376
Eco130I CCWWGG 1 cut(s) 597
Eco24I GRGCYC 1 cut(s) 426
Eco31I GGTCTC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 424
EcoRI GAATTC 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 597
EcoT38I GRGCYC 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 597
FaeI CATG 5 cut(s) 118, 214, 392, 601, 613
FalI AAGNNNNNCTT 2 cut(s) 476, 508
FatI CATG 5 cut(s) 114, 210, 388, 597, 609
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 2 cut(s) 521, 616
FokI GGATG 2 cut(s) 278, 374
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 2 cut(s) 521, 616
FspBI CTAG 2 cut(s) 63, 164
GluI GCNGC 2 cut(s) 521, 616
GsaI CCCAGC 1 cut(s) 358
HaeIII GGCC 1 cut(s) 537
HgaI GACGC 1 cut(s) 68
Hin1II CATG 5 cut(s) 118, 214, 392, 601, 613
HinfI GANTC 6 cut(s) 35, 333, 410, 428, 446, 648
Hpy166II GTNNAC 2 cut(s) 289, 373
Hpy188I TCNGA 5 cut(s) 122, 265, 321, 442, 511
Hpy188III TCNNGA 6 cut(s) 32, 83, 337, 389, 414, 571
Hpy8I GTNNAC 2 cut(s) 289, 373
HpyAV CCTTC 3 cut(s) 334, 411, 638
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 2 cut(s) 133, 375
HpyCH4V TGCA 3 cut(s) 223, 407, 615
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 624
HpyF3I CTNAG 5 cut(s) 76, 119, 249, 508, 663
HpySE526I ACGT 2 cut(s) 133, 375
Hsp92II CATG 5 cut(s) 118, 214, 392, 601, 613
Kzo9I GATC 3 cut(s) 66, 207, 486
LmnI GCTCC 2 cut(s) 421, 517
LpnPI CCDG 8 cut(s) 78, 157, 322, 344, 368, 437, 540, 551
Lsp1109I GCAGC 2 cut(s) 507, 627
LweI GCATC 3 cut(s) 325, 352, 373
MaeI CTAG 2 cut(s) 63, 164
MaeII ACGT 2 cut(s) 133, 375
MalI GATC 3 cut(s) 68, 209, 488
MbiI CCGCTC 1 cut(s) 326
MboI GATC 3 cut(s) 66, 207, 486
MboII GAAGA 5 cut(s) 132, 143, 171, 425, 455
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 426
MluCI AATT 2 cut(s) 27, 378
MlyI GAGTC 3 cut(s) 342, 419, 455
MnlI CCTC 5 cut(s) 271, 505, 508, 517, 595
MslI CAYNNNNRTG 2 cut(s) 585, 602
MspA1I CMGCKG 1 cut(s) 358
MwoI GCNNNNNNNGC 2 cut(s) 115, 624
NcoI CCATGG 1 cut(s) 597
NdeII GATC 3 cut(s) 66, 207, 486
NlaIII CATG 5 cut(s) 118, 214, 392, 601, 613
NmeAIII GCCGAG 1 cut(s) 513
NspI RCATGY 1 cut(s) 118
PagI TCATGA 1 cut(s) 388
PfeI GAWTC 3 cut(s) 35, 428, 648
PkrI GCNGC 2 cut(s) 522, 617
PleI GAGTC 3 cut(s) 341, 418, 454
PpsI GAGTC 3 cut(s) 341, 418, 454
Ppu21I YACGTR 2 cut(s) 134, 376
Psp124BI GAGCTC 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 354
PstNI CAGNNNCTG 1 cut(s) 336
PsuI RGATCY 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 358
RsaI GTAC 3 cut(s) 132, 136, 578
RsaNI GTAC 3 cut(s) 131, 135, 577
RseI CAYNNNNRTG 2 cut(s) 585, 602
SacI GAGCTC 1 cut(s) 426
SatI GCNGC 2 cut(s) 521, 616
Sau3AI GATC 3 cut(s) 66, 207, 486
SchI GAGTC 3 cut(s) 342, 419, 455
SduI GDGCHC 1 cut(s) 426
SfaNI GCATC 3 cut(s) 325, 352, 373
SfcI CTRYAG 1 cut(s) 366
SmiMI CAYNNNNRTG 2 cut(s) 585, 602
SnaBI TACGTA 2 cut(s) 134, 376
Sse9I AATT 2 cut(s) 27, 378
SsiI CCGC 2 cut(s) 324, 592
SspMI CTAG 2 cut(s) 63, 164
SstI GAGCTC 1 cut(s) 426
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 2 cut(s) 136, 378
TaqI TCGA 5 cut(s) 206, 413, 426, 489, 672
TasI AATT 2 cut(s) 27, 378
TfiI GAWTC 3 cut(s) 35, 428, 648
TscAI CASTG 1 cut(s) 465
TseI GCWGC 2 cut(s) 520, 615
TspDTI ATGAA 3 cut(s) 143, 190, 598
TspRI CASTG 1 cut(s) 465
XapI RAATTY 1 cut(s) 27
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 2 cut(s) 63, 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.