MD16G1157800.v1.1
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017489
Physical Location & Seq
Forward (+)
12637580 .. 12639236
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1157800.v1.1.491

Sequence Viewer

Length: 582 bp
ATGTTTCTGGTGGATTGGTTCTATGGAGTTCTGGCGTCGCTTGGGCTATGGCAGAAGGAGGCCAAGATCTTGTTCTTAGGGCTCGATAATGCCGGAAAGACCACCTTGCTTCACATGCTCAAAGACGAGAGATTAGTTCAGCACCAACCGACGCAGCATCCGACGTCGGAGGAGCTGAGTATTGGGAAGATTAAGTTCAAGGCCTTTGATTTGGGAGGGCATCAGGTCGCTCGCAGAGTTTGGAAGGACTATTACGCCAAGGTGGATTCTGTGGTCTACCTGGTTGATGCTTACGACAAGGAGAGATTTGCAGAGTCAAAGAAGGAGCTCGATGCTCTTCTCTCTGACGAGTCATTGGCAACTGCCCCGTTTCTTATCTTGGGAAACAAGATCGACATACCTTATGCTGCATCAGAGGATGAGCTGCGTTACCATTTGGGCCTGACTAACTTCACCACAGGCAAGGGGAAGGTCAATCTGACCGATTCCAATGTCCGCCCGCTTGAGGTGTTCATGTGCAGCATTGTCCGCAAGATGGGGTACGGTGAAGGCTTCAAGTGGCTTTCACAATACATAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

194

Amino Acids

22.01

Weight (kDa)

6.97

Isoelectric Point (pI)

25.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 6.5e-65 ADP-ribosylation factor family
SRPRB PF09439 19 - 144 1.2e-07 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 3.6e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 1.6e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 3.3e-06 Ras family
G-alpha PF00503 49 - 133 1.3e-08 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02080
fragaria_vesca FvH4_4g19410
malus_domestica MD16G1157800.v1.1
pyrus_communis pycom13g13440
rosa_chinensis RchiOBHm_Chr4g0424441
rosa_laevigata RLG00000007474
rosa_multiflora Rmu_sc0003567.1_g000010
rosa_roxburghii Rroxscaffold_5G00366380
rosa_samantha Rh4AG253900 Rh4BG259200 Rh4CG270500 Rh4DG253700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 276
AciI CCGC 3 cut(s) 496, 500, 529
AcyI GRCGYC 2 cut(s) 35, 164
AfaI GTAC 1 cut(s) 542
AfiI CCNNNNNNNGG 2 cut(s) 505, 535
AgsI TTSAA 2 cut(s) 199, 556
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 3 cut(s) 175, 328, 424
AluI AGCT 3 cut(s) 175, 328, 424
Alw21I GWGCWC 1 cut(s) 330
AoxI GGCC 3 cut(s) 60, 201, 439
ApeKI GCWGC 4 cut(s) 154, 407, 424, 519
AspS9I GGNCC 1 cut(s) 439
AsuHPI GGTGA 2 cut(s) 445, 557
BanII GRGCYC 2 cut(s) 84, 330
BarI GAAGNNNNNNTAC 2 cut(s) 236, 268
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 4 cut(s) 166, 394, 411, 531
BccI CCATC 1 cut(s) 529
BciT130I CCWGG 1 cut(s) 281
BglII AGATCT 1 cut(s) 66
BisI GCNGC 4 cut(s) 155, 408, 425, 520
BlsI GCNGC 4 cut(s) 156, 409, 426, 521
Bme1390I CCNGG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 5 cut(s) 166, 229, 277, 322, 419
BpuEI CTTGAG 1 cut(s) 524
BsaHI GRCGYC 2 cut(s) 35, 164
BsaJI CCNNGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 535
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 2 cut(s) 157, 424
BseLI CCNNNNNNNGG 2 cut(s) 505, 535
BseMII CTCAG 1 cut(s) 167
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 4 cut(s) 166, 394, 411, 531
BsgI GTGCAG 1 cut(s) 538
BshFI GGCC 3 cut(s) 62, 203, 441
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 2 cut(s) 505, 535
BsnI GGCC 3 cut(s) 62, 203, 441
Bsp1286I GDGCHC 2 cut(s) 84, 330
Bsp143I GATC 2 cut(s) 66, 390
BspACI CCGC 3 cut(s) 496, 500, 529
BspANI GGCC 3 cut(s) 62, 203, 441
BspCNI CTCAG 1 cut(s) 168
BspQI GCTCTTC 1 cut(s) 342
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 2 cut(s) 66, 390
BssNI GRCGYC 2 cut(s) 35, 164
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 545
Bst6I CTCTTC 1 cut(s) 342
BstACI GRCGYC 2 cut(s) 35, 164
BstC8I GCNNGC 2 cut(s) 232, 500
BstDEI CTNAG 2 cut(s) 76, 176
BstF5I GGATG 2 cut(s) 157, 424
BstKTI GATC 2 cut(s) 69, 393
BstMBI GATC 2 cut(s) 66, 390
BstMWI GCNNNNNNNGC 2 cut(s) 115, 528
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 279
BstV1I GCAGC 4 cut(s) 166, 394, 411, 531
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 3 cut(s) 62, 203, 441
BtsCI GGATG 2 cut(s) 157, 424
Cac8I GCNNGC 2 cut(s) 232, 500
Cfr13I GGNCC 1 cut(s) 439
CseI GACGC 2 cut(s) 24, 160
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 1 cut(s) 541
CviAII CATG 2 cut(s) 115, 514
CviQI GTAC 1 cut(s) 541
DdeI CTNAG 2 cut(s) 76, 176
DpnI GATC 2 cut(s) 68, 392
DpnII GATC 2 cut(s) 66, 390
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
EciI GGCGGA 1 cut(s) 485
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 258
Eco147I AGGCCT 1 cut(s) 203
Eco24I GRGCYC 2 cut(s) 84, 330
Eco53kI GAGCTC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 2 cut(s) 84, 330
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 2 cut(s) 118, 517
FaiI YATR 7 cut(s) 24, 49, 116, 398, 405, 515, 575
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FatI CATG 2 cut(s) 114, 513
FauI CCCGC 1 cut(s) 507
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 4 cut(s) 155, 408, 425, 520
FokI GGATG 2 cut(s) 144, 431
FriOI GRGCYC 2 cut(s) 84, 330
Fsp4HI GCNGC 4 cut(s) 155, 408, 425, 520
GluI GCNGC 4 cut(s) 155, 408, 425, 520
HaeIII GGCC 3 cut(s) 62, 203, 441
HapII CCGG 1 cut(s) 93
HgaI GACGC 2 cut(s) 24, 160
Hin1I GRCGYC 2 cut(s) 35, 164
Hin1II CATG 2 cut(s) 118, 517
HinfI GANTC 4 cut(s) 266, 314, 350, 485
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 445, 557
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 5 cut(s) 162, 169, 346, 415, 480
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 4 cut(s) 40, 154, 166, 169
HpyAV CCTTC 5 cut(s) 49, 238, 316, 463, 542
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 3 cut(s) 311, 410, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 528
HpyF3I CTNAG 2 cut(s) 76, 176
HpySE526I ACGT 1 cut(s) 164
Hsp92I GRCGYC 2 cut(s) 35, 164
Hsp92II CATG 2 cut(s) 118, 517
Kzo9I GATC 2 cut(s) 66, 390
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 2 cut(s) 172, 325
LpnPI CCDG 7 cut(s) 17, 106, 209, 266, 293, 444, 455
Lsp1109I GCAGC 4 cut(s) 166, 394, 411, 531
LweI GCATC 5 cut(s) 166, 229, 277, 322, 419
MabI ACCWGGT 1 cut(s) 279
MaeII ACGT 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 428
MalI GATC 2 cut(s) 68, 392
MboI GATC 2 cut(s) 66, 390
MboII GAAGA 2 cut(s) 199, 329
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 2 cut(s) 84, 330
MlyI GAGTC 2 cut(s) 323, 359
MmeI TCCRAC 2 cut(s) 147, 185
MnlI CCTC 5 cut(s) 52, 163, 209, 409, 499
MseI TTAA 1 cut(s) 192
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 2 cut(s) 115, 528
NdeII GATC 2 cut(s) 66, 390
NlaIII CATG 2 cut(s) 118, 517
NspI RCATGY 1 cut(s) 118
PceI AGGCCT 1 cut(s) 203
PciSI GCTCTTC 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PfeI GAWTC 2 cut(s) 266, 485
PkrI GCNGC 4 cut(s) 156, 409, 426, 521
PleI GAGTC 2 cut(s) 322, 358
PpsI GAGTC 2 cut(s) 322, 358
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspPI GGNCC 1 cut(s) 439
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 542
RsaNI GTAC 1 cut(s) 541
SacI GAGCTC 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 4 cut(s) 155, 408, 425, 520
Sau3AI GATC 2 cut(s) 66, 390
Sau96I GGNCC 1 cut(s) 439
SchI GAGTC 2 cut(s) 323, 359
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 2 cut(s) 84, 330
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 5 cut(s) 166, 229, 277, 322, 419
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 3 cut(s) 496, 500, 529
SstI GAGCTC 1 cut(s) 330
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 1 cut(s) 167
TaqI TCGA 3 cut(s) 84, 330, 393
TaqII GACCGA 1 cut(s) 497
TfiI GAWTC 2 cut(s) 266, 485
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 4 cut(s) 154, 407, 424, 519
TspDTI ATGAA 1 cut(s) 502
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 276
ZraI GACGTC 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.