Rh4CG270500
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0017489
Physical Location & Seq
Reverse (-)
52498539 .. 52499357
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG270500.1

Sequence Viewer

Length: 582 bp
ATGTTTCTTGTGGATTGGTTCTATGGAATTCTTGAATCGTTGGGGCTATGGCATAAAGACGCTAGGATCTGTTTCTTAGGTCTTGATAATGCTGGAAAGACCACTTTGCTTCACATGCTCAGATTTGGGAGCTTGAAGCAAAGCGAACCAACTCAGCAACCAACCTCGGATGATTTTTCAATAGGGAGTGTCCACTTCACAGCCTTTGATTTGGGTGGGCATCAGACCGCTCGCAGAGTCTGGAAGGATTACTATGCCCAGCTGGATGCTGTAGTCTACGTAATTGATGCTCATGACAAGGAAAGATTTGCAGAGTCGAGAAGGGAGCTCGATTCTCTTCTCTCTGAAGAGTCTCTGGCAACAGTGCCGTTTCTTATTTTGGGAAACAAGATCGACTTGCCTTATGCTGCCTCAGAGGAGGAGCTGCGTGCTTCTCTCGGCCTGACCAACTTCACAACTGGCAAGGGTAAGGTCAAGATGTACCATTCCAATGTCCGCCCCATGGAGGTGTTCATGTGCAGCATTGCCCGCAAGATGGGTTATGGAGAAGGATTCAAGTGGCTTGCTCAGTACATCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

193

Amino Acids

21.97

Weight (kDa)

5.88

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 1.6e-59 ADP-ribosylation factor family
SRPRB PF09439 20 - 146 9.2e-09 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 4.3e-07 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 2.5e-06 50S ribosome-binding GTPase
Ras PF00071 23 - 135 3.5e-06 Ras family
G-alpha PF00503 50 - 133 1.3e-07 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02080
fragaria_vesca FvH4_4g19410
malus_domestica MD16G1157800.v1.1
pyrus_communis pycom13g13440
rosa_chinensis RchiOBHm_Chr4g0424441
rosa_laevigata RLG00000007474
rosa_multiflora Rmu_sc0003567.1_g000010
rosa_roxburghii Rroxscaffold_5G00366380
rosa_samantha Rh4AG253900 Rh4BG259200 Rh4CG270500 Rh4DG253700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 230
AccI GTMKAC 1 cut(s) 276
AciI CCGC 3 cut(s) 228, 496, 529
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 1 cut(s) 27
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 2 cut(s) 482, 572
AfiI CCNNNNNNNGG 3 cut(s) 502, 505, 535
AgsI TTSAA 4 cut(s) 35, 136, 180, 556
AluBI AGCT 4 cut(s) 132, 262, 328, 424
AluI AGCT 4 cut(s) 132, 262, 328, 424
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 357
AlwI GGATC 1 cut(s) 74
AlwNI CAGNNNCTG 1 cut(s) 240
AoxI GGCC 1 cut(s) 439
ApeKI GCWGC 3 cut(s) 407, 424, 519
ApoI RAATTY 1 cut(s) 27
BanII GRGCYC 1 cut(s) 330
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 3 cut(s) 394, 411, 531
BccI CCATC 1 cut(s) 529
BceAI ACGGC 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 357
BfaI CTAG 1 cut(s) 63
BfmI CTRYAG 1 cut(s) 270
BisI GCNGC 3 cut(s) 408, 425, 520
BlsI GCNGC 3 cut(s) 409, 426, 521
BmsI GCATC 3 cut(s) 229, 256, 277
BsaAI YACGTR 1 cut(s) 280
BsaJI CCNNGG 2 cut(s) 165, 501
Bsc4I CCNNNNNNNGG 3 cut(s) 502, 505, 535
Bse1I ACTGG 1 cut(s) 463
Bse3DI GCAATG 1 cut(s) 522
BseDI CCNNGG 2 cut(s) 165, 501
BseGI GGATG 2 cut(s) 175, 271
BseLI CCNNNNNNNGG 3 cut(s) 502, 505, 535
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 4 cut(s) 133, 167, 426, 581
BseNI ACTGG 1 cut(s) 463
BseRI GAGGAG 2 cut(s) 431, 434
BseXI GCAGC 3 cut(s) 394, 411, 531
BseYI CCCAGC 1 cut(s) 258
BsgI GTGCAG 1 cut(s) 538
BshFI GGCC 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 330
BslI CCNNNNNNNGG 3 cut(s) 502, 505, 535
BsmAI GTCTC 1 cut(s) 357
BsnI GGCC 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 2 cut(s) 66, 390
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 3 cut(s) 228, 496, 529
BspANI GGCC 1 cut(s) 441
BspCNI CTCAG 4 cut(s) 132, 166, 425, 580
BspHI TCATGA 1 cut(s) 292
BspPI GGATC 1 cut(s) 74
BsrBI CCGCTC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 522
BsrI ACTGG 1 cut(s) 463
BssECI CCNNGG 2 cut(s) 165, 501
BssMI GATC 2 cut(s) 66, 390
BssT1I CCWWGG 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 2 cut(s) 342, 342
BstBAI YACGTR 1 cut(s) 280
BstC8I GCNNGC 4 cut(s) 232, 429, 529, 564
BstDEI CTNAG 5 cut(s) 76, 119, 153, 412, 567
BstDSI CCRYGG 1 cut(s) 501
BstF5I GGATG 2 cut(s) 175, 271
BstKTI GATC 2 cut(s) 69, 393
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 2 cut(s) 66, 390
BstMWI GCNNNNNNNGC 2 cut(s) 115, 528
BstNSI RCATGY 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 270
BstSNI TACGTA 1 cut(s) 280
BstV1I GCAGC 3 cut(s) 394, 411, 531
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 1 cut(s) 441
BtgI CCRYGG 1 cut(s) 501
BtsCI GGATG 2 cut(s) 175, 271
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 4 cut(s) 232, 429, 529, 564
CaiI CAGNNNCTG 1 cut(s) 240
CciI TCATGA 1 cut(s) 292
CseI GACGC 1 cut(s) 68
Csp6I GTAC 2 cut(s) 481, 571
CviAII CATG 4 cut(s) 115, 293, 502, 514
CviJI RGCY 8 cut(s) 46, 132, 203, 262, 328, 424, 441, 562
CviKI_1 RGCY 8 cut(s) 46, 132, 203, 262, 328, 424, 441, 562
CviQI GTAC 2 cut(s) 481, 571
DdeI CTNAG 5 cut(s) 76, 119, 153, 412, 567
DpnI GATC 2 cut(s) 68, 392
DpnII GATC 2 cut(s) 66, 390
Eam1104I CTCTTC 2 cut(s) 342, 342
EarI CTCTTC 2 cut(s) 342, 342
EciI GGCGGA 1 cut(s) 485
Ecl136II GAGCTC 1 cut(s) 328
Eco105I TACGTA 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 328
EcoRI GAATTC 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 4 cut(s) 118, 296, 505, 517
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FatI CATG 4 cut(s) 114, 292, 501, 513
FauI CCCGC 1 cut(s) 536
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 3 cut(s) 408, 425, 520
FokI GGATG 2 cut(s) 182, 278
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 3 cut(s) 408, 425, 520
FspBI CTAG 1 cut(s) 63
GluI GCNGC 3 cut(s) 408, 425, 520
GsaI CCCAGC 1 cut(s) 262
HaeIII GGCC 1 cut(s) 441
HgaI GACGC 1 cut(s) 68
Hin1II CATG 4 cut(s) 118, 296, 505, 517
HinfI GANTC 6 cut(s) 35, 237, 314, 332, 350, 552
Hpy166II GTNNAC 2 cut(s) 193, 277
Hpy188I TCNGA 5 cut(s) 122, 169, 225, 346, 415
Hpy188III TCNNGA 6 cut(s) 32, 83, 241, 293, 318, 475
Hpy8I GTNNAC 2 cut(s) 193, 277
HpyAV CCTTC 3 cut(s) 238, 315, 542
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 2 cut(s) 311, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 528
HpyF3I CTNAG 5 cut(s) 76, 119, 153, 412, 567
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 4 cut(s) 118, 296, 505, 517
Kzo9I GATC 2 cut(s) 66, 390
LmnI GCTCC 3 cut(s) 129, 325, 421
LpnPI CCDG 7 cut(s) 78, 226, 248, 272, 341, 444, 455
Lsp1109I GCAGC 3 cut(s) 394, 411, 531
LweI GCATC 3 cut(s) 229, 256, 277
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 279
MalI GATC 2 cut(s) 68, 392
MbiI CCGCTC 1 cut(s) 230
MboI GATC 2 cut(s) 66, 390
MboII GAAGA 2 cut(s) 329, 359
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 2 cut(s) 27, 282
MlyI GAGTC 3 cut(s) 246, 323, 359
MnlI CCTC 5 cut(s) 175, 409, 412, 421, 499
MslI CAYNNNNRTG 2 cut(s) 489, 506
MspA1I CMGCKG 1 cut(s) 262
MwoI GCNNNNNNNGC 2 cut(s) 115, 528
NcoI CCATGG 1 cut(s) 501
NdeII GATC 2 cut(s) 66, 390
NlaIII CATG 4 cut(s) 118, 296, 505, 517
NmeAIII GCCGAG 1 cut(s) 417
NspI RCATGY 1 cut(s) 118
PagI TCATGA 1 cut(s) 292
PfeI GAWTC 3 cut(s) 35, 332, 552
PkrI GCNGC 3 cut(s) 409, 426, 521
PleI GAGTC 3 cut(s) 245, 322, 358
PpsI GAGTC 3 cut(s) 245, 322, 358
Ppu21I YACGTR 1 cut(s) 280
Psp124BI GAGCTC 1 cut(s) 330
PspFI CCCAGC 1 cut(s) 258
PstNI CAGNNNCTG 1 cut(s) 240
PsuI RGATCY 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 262
RsaI GTAC 2 cut(s) 482, 572
RsaNI GTAC 2 cut(s) 481, 571
RseI CAYNNNNRTG 2 cut(s) 489, 506
SacI GAGCTC 1 cut(s) 330
SatI GCNGC 3 cut(s) 408, 425, 520
Sau3AI GATC 2 cut(s) 66, 390
SchI GAGTC 3 cut(s) 246, 323, 359
SduI GDGCHC 1 cut(s) 330
SetI ASST 9 cut(s) 82, 134, 167, 264, 282, 330, 426, 474, 510
SfaNI GCATC 3 cut(s) 229, 256, 277
SfcI CTRYAG 1 cut(s) 270
SmiMI CAYNNNNRTG 2 cut(s) 489, 506
SnaBI TACGTA 1 cut(s) 280
Sse9I AATT 2 cut(s) 27, 282
SsiI CCGC 3 cut(s) 228, 496, 529
SspMI CTAG 1 cut(s) 63
SstI GAGCTC 1 cut(s) 330
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 1 cut(s) 282
TaqI TCGA 4 cut(s) 317, 330, 393, 576
TasI AATT 2 cut(s) 27, 282
TatI WGTACW 1 cut(s) 570
TfiI GAWTC 3 cut(s) 35, 332, 552
TscAI CASTG 1 cut(s) 369
TseI GCWGC 3 cut(s) 407, 424, 519
TspDTI ATGAA 1 cut(s) 502
TspRI CASTG 1 cut(s) 369
XapI RAATTY 1 cut(s) 27
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.