AT4G02250

Invertase pectin methylesterase inhibitor family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015173
Physical Location & Seq
Forward (+)
983970 .. 984523
554 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02250.1

Sequence Viewer

Length: 483 bp
ATGAAAATGGCCGGAAGGATCTTTCTCCTTTTCCTCTCCGTCTACGTCACGGTGGCCATCGCCGACAAAGCGTTTTGCGTTGCAAGCCTAACGTCCCGTCCTGAGGCAGCAACCGCAACCGCACCAAAACTAGGCGTGATCGCGTTAAGCATCGCGTCCTCAAATGCTTCGGACACATCTTTCTACATCAAAGCCAAACTAAAGCAAAAGAACCTCGAGCCGGCTTTAGAAGACACCCTCGACGATTGCTCTAAGAATTACTTAGATGCGGTCGCGCAGCTCGACGATTCTCTAGCCGCTTTGATGCAAAACTCGTTCATCGACGTCGATATTTGGCTCAACACCGCGATAAGCGACGGTGAGGCTTGTGAAAACGCTTTGAACGATCGTGCCGGTAATGATGCTGAGCTTGCTCGTAGGAACACAAACTTGTTGAAGCTTTGCAAGGATGCTCTTCTCATTAACACTATTTTAACTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

160

Amino Acids

17.2

Weight (kDa)

4.67

Isoelectric Point (pI)

23.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 22 - 153 2.2e-21 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55770 AT4G02250
fragaria_vesca FvH4_2g25950
malus_domestica MD03G1290800.v1.1 MD11G1307600.v1.1
rosa_chinensis RchiOBHm_Chr6g0294271
rosa_laevigata RLG00000011882
rosa_multiflora Rmu_co8483625.1_g000001 Rmu_sc0003996.1_g000007
rosa_roxburghii Rroxscaffold_7G00173490
rosa_rugosa Rorug06G0243400
rosa_samantha Rh6AG355600 Rh6BG362500 Rh6CG369600 Rh6DG356400
rosa_wichuraiana Rw6G030980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 327
AccI GTMKAC 1 cut(s) 42
AccII CGCG 4 cut(s) 143, 155, 275, 347
AciI CCGC 5 cut(s) 114, 120, 269, 297, 345
AclWI GGATC 1 cut(s) 26
AcoI YGGCCR 2 cut(s) 9, 54
AcyI GRCGYC 1 cut(s) 324
AfiI CCNNNNNNNGG 3 cut(s) 103, 131, 220
AgsI TTSAA 2 cut(s) 382, 436
AluBI AGCT 3 cut(s) 280, 409, 439
AluI AGCT 3 cut(s) 280, 409, 439
AlwI GGATC 1 cut(s) 26
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 2 cut(s) 9, 54
ApeKI GCWGC 2 cut(s) 107, 277
AspLEI GCGC 1 cut(s) 277
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 215
AxyI CCTNAGG 1 cut(s) 102
BalI TGGCCA 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 119, 289
BccI CCATC 1 cut(s) 65
BfaI CTAG 2 cut(s) 131, 293
BisI GCNGC 3 cut(s) 108, 278, 297
BlpI GCTNAGC 1 cut(s) 405
BlsI GCNGC 3 cut(s) 109, 279, 298
BmeT110I CYCGRG 1 cut(s) 215
BmsI GCATC 5 cut(s) 159, 256, 294, 391, 439
BpiI GAAGAC 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 324
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 131, 220
Bse118I RCCGGY 2 cut(s) 220, 392
Bse21I CCTNAGG 1 cut(s) 102
BseGI GGATG 1 cut(s) 454
BseLI CCNNNNNNNGG 3 cut(s) 103, 131, 220
BseMII CTCAG 2 cut(s) 93, 396
BseXI GCAGC 2 cut(s) 119, 289
Bsh1236I CGCG 4 cut(s) 143, 155, 275, 347
Bsh1285I CGRYCG 2 cut(s) 273, 388
BshFI GGCC 2 cut(s) 11, 56
BsiEI CGRYCG 2 cut(s) 273, 388
BsiHKCI CYCGRG 1 cut(s) 215
BsiSI CCGG 3 cut(s) 12, 221, 393
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 3 cut(s) 103, 131, 220
BsmFI GGGAC 1 cut(s) 79
BsnI GGCC 2 cut(s) 11, 56
BsoBI CYCGRG 1 cut(s) 215
Bsp143I GATC 3 cut(s) 18, 138, 385
Bsp1720I GCTNAGC 1 cut(s) 405
BspACI CCGC 5 cut(s) 114, 120, 269, 297, 345
BspANI GGCC 2 cut(s) 11, 56
BspCNI CTCAG 2 cut(s) 94, 397
BspFNI CGCG 4 cut(s) 143, 155, 275, 347
BspPI GGATC 1 cut(s) 26
BspQI GCTCTTC 1 cut(s) 459
BsrFI RCCGGY 2 cut(s) 220, 392
BssAI RCCGGY 2 cut(s) 220, 392
BssMI GATC 3 cut(s) 18, 138, 385
BssNI GRCGYC 1 cut(s) 324
Bst4CI ACNGT 2 cut(s) 52, 359
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 1 cut(s) 324
BstC8I GCNNGC 3 cut(s) 85, 222, 411
BstDEI CTNAG 4 cut(s) 102, 252, 262, 405
BstF5I GGATG 1 cut(s) 454
BstFNI CGCG 4 cut(s) 143, 155, 275, 347
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 3 cut(s) 21, 141, 388
BstMBI GATC 3 cut(s) 18, 138, 385
BstMCI CGRYCG 2 cut(s) 273, 388
BstMWI GCNNNNNNNGC 4 cut(s) 68, 84, 113, 410
BstUI CGCG 4 cut(s) 143, 155, 275, 347
BstV1I GCAGC 2 cut(s) 119, 289
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
Bsu36I CCTNAGG 1 cut(s) 102
BsuRI GGCC 2 cut(s) 11, 56
BtgZI GCGATG 2 cut(s) 43, 136
BtsCI GGATG 1 cut(s) 454
Cac8I GCNNGC 3 cut(s) 85, 222, 411
CfoI GCGC 1 cut(s) 277
Cfr10I RCCGGY 2 cut(s) 220, 392
CseI GACGC 1 cut(s) 144
DdeI CTNAG 4 cut(s) 102, 252, 262, 405
DpnI GATC 3 cut(s) 20, 140, 387
DpnII GATC 3 cut(s) 18, 138, 385
EaeI YGGCCR 2 cut(s) 9, 54
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Eco81I CCTNAGG 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 215
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 79
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 108, 278, 297
FokI GGATG 1 cut(s) 461
Fsp4HI GCNGC 3 cut(s) 108, 278, 297
FspBI CTAG 2 cut(s) 131, 293
GlaI GCGC 1 cut(s) 276
GluI GCNGC 3 cut(s) 108, 278, 297
HaeIII GGCC 2 cut(s) 11, 56
HapII CCGG 3 cut(s) 12, 221, 393
HgaI GACGC 1 cut(s) 144
HhaI GCGC 1 cut(s) 277
Hin1I GRCGYC 1 cut(s) 324
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 1 cut(s) 287
HpaII CCGG 3 cut(s) 12, 221, 393
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 1 cut(s) 172
Hpy188III TCNNGA 1 cut(s) 101
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 5 cut(s) 245, 287, 326, 329, 359
HpyAV CCTTC 1 cut(s) 9
HpyCH4III ACNGT 2 cut(s) 52, 359
HpyCH4IV ACGT 3 cut(s) 45, 92, 324
HpyCH4V TGCA 3 cut(s) 83, 307, 444
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 84, 113, 410
HpyF3I CTNAG 4 cut(s) 102, 252, 262, 405
HpySE526I ACGT 3 cut(s) 45, 92, 324
Hsp92I GRCGYC 1 cut(s) 324
HspAI GCGC 1 cut(s) 275
KroI GCCGGC 1 cut(s) 220
KroNI GCCGGC 1 cut(s) 222
Kzo9I GATC 3 cut(s) 18, 138, 385
LguI GCTCTTC 1 cut(s) 459
LpnPI CCDG 4 cut(s) 25, 114, 234, 406
Lsp1109I GCAGC 2 cut(s) 119, 289
LweI GCATC 5 cut(s) 159, 256, 294, 391, 439
MaeI CTAG 2 cut(s) 131, 293
MaeII ACGT 3 cut(s) 45, 92, 324
MaeIII GTNAC 1 cut(s) 46
MalI GATC 3 cut(s) 20, 140, 387
MboI GATC 3 cut(s) 18, 138, 385
MboII GAAGA 2 cut(s) 242, 446
MflI RGATCY 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 1 cut(s) 256
MluNI TGGCCA 1 cut(s) 56
MnlI CCTC 6 cut(s) 44, 97, 169, 224, 248, 355
Mox20I TGGCCA 1 cut(s) 56
MroNI GCCGGC 1 cut(s) 220
MscI TGGCCA 1 cut(s) 56
MseI TTAA 3 cut(s) 146, 462, 473
Msp20I TGGCCA 1 cut(s) 56
MspI CCGG 3 cut(s) 12, 221, 393
MvnI CGCG 4 cut(s) 143, 155, 275, 347
MwoI GCNNNNNNNGC 4 cut(s) 68, 84, 113, 410
NaeI GCCGGC 1 cut(s) 222
NdeII GATC 3 cut(s) 18, 138, 385
NgoMIV GCCGGC 1 cut(s) 220
NmuCI GTSAC 1 cut(s) 46
PaeR7I CTCGAG 1 cut(s) 215
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 2 cut(s) 279, 381
PdiI GCCGGC 1 cut(s) 222
PfeI GAWTC 1 cut(s) 287
PkrI GCNGC 3 cut(s) 109, 279, 298
Ple19I CGATCG 1 cut(s) 388
PspXI VCTCGAGB 1 cut(s) 215
PsuI RGATCY 1 cut(s) 18
PvuI CGATCG 1 cut(s) 388
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 3 cut(s) 146, 462, 473
SatI GCNGC 3 cut(s) 108, 278, 297
Sau3AI GATC 3 cut(s) 18, 138, 385
SetI ASST 7 cut(s) 48, 95, 216, 282, 327, 411, 441
SfaNI GCATC 5 cut(s) 159, 256, 294, 391, 439
Sfr274I CTCGAG 1 cut(s) 215
SlaI CTCGAG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 1 cut(s) 256
SsiI CCGC 5 cut(s) 114, 120, 269, 297, 345
SspMI CTAG 2 cut(s) 131, 293
TaaI ACNGT 2 cut(s) 52, 359
TaiI ACGT 3 cut(s) 48, 95, 327
TaqI TCGA 5 cut(s) 216, 240, 282, 321, 327
TasI AATT 1 cut(s) 256
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 3 cut(s) 146, 462, 473
Tru9I TTAA 3 cut(s) 146, 462, 473
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 2 cut(s) 107, 277
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 2 cut(s) 17, 307
TspGWI ACGGA 1 cut(s) 28
XhoI CTCGAG 1 cut(s) 215
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 2 cut(s) 131, 293
ZraI GACGTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.